Fluazinam-containing pesticide composition as well as seed treatment suspending agent, preparation method and application thereof

Abstract

The invention relates to a fluazinam-containing pesticide composition, a seed treatment suspending agent as well as a preparation method and application of the seed treatment suspending agent. The invention provides a fluazinam-containing pesticide composition. The active ingredients of the fluazinam-containing pesticide composition comprise fluazinam, fludioxonil and clothianidin, the mass ratio of the clothianidin to the fluazinam to the fludioxonil is (6-7): (0.4-0.8): (0.2-0.6), and the composition has an excellent synergistic effect. The suspending agent for seed treatment is compounded with a film-forming agent composed of acrylate and polydimethylsiloxane according to a specific proportion, it can be ensured that seed coats are not prone to falling off after being coated, meanwhile, adjustment can be conducted according to environmental adaptability, a coating layer is evenly mixed with a pesticide and pigment, the release time of effective components of pesticides is prolonged, and the pesticide effect is improved. The problem of seed coating fading is effectively solved, and the pesticide composition has an excellent effect in prevention and treatment of agricultural diseases such as peanut root rot, grub, wheat stem rot and aphid.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of pesticides, and discloses a pesticide composition containing fluazinam, a seed treatment suspension concentrate of the pesticide composition, a preparation method and application. BACKGROUND

[0002] Fluazinam is a new pyridine amide broad-spectrum fungicide with protective, curative and eradicative effects. Its mechanism of action is to inhibit complex III (cytochrome bcl complex) on the inner membrane of pathogen mitochondria, block the oxidative phosphorylation process to destroy the energy metabolism of the pathogen. However, single use of fluazinam is prone to cause decline in control effect due to accumulation of pathogen resistance, limited action spectrum or environmental factors (such as rainwater washing and temperature fluctuation), and therefore needs to be scientifically compounded with other components to form a pesticide composition with synergistic effect, so as to maximize the control effect. In the currently reported pesticide compositions of fluazinam, there are few synergistic effects, and most of them are additive effects. Therefore, in order to meet the agricultural needs, there is an urgent need for a pesticide composition synergistic with fluazinam.

[0003] In agricultural production, seeds are the starting point of crop growth, and the health conditions such as seed germination and bud robustness are related to the entire growth cycle and final yield of crops. In order to promote seed germination and growth, reduce the occurrence of diseases and pests, and promote the healthy breeding of crops, seed treatment agents have emerged as the times require. As a kind of chemical preparation commonly used for seed treatment in agricultural production, seed treatment suspension concentrate is the most popular dosage form at present, which can provide necessary disease and pest control for seed germination and early growth, and is equivalent to wrapping a layer of defense barrier for seeds, so as to promote the smooth growth of crops and even achieve high yield.

[0004] However, the core technical difficulty of seed treatment suspension concentrate lies in the selection and performance regulation of film-forming agent. The ideal film-forming agent needs to form a uniform and firm functional drug film on the surface of the seed, which is like a protective "clothing" for the seed. This film must achieve multiple balances: accurate control of film thickness: too thick film will hinder seed respiration and inhibit germination; too thin film will lead to premature release of the drug, which cannot provide sustained protection for seed germination and seedling growth in the critical period, and makes the seed vulnerable to diseases and pests; strong environmental adaptability of film-forming property: the film-forming property needs to be stable and not easily affected by environmental factors (such as low temperature) to cause poor film-forming or coating shedding, so as to ensure consistent performance under different climates and field conditions; effective synergy with functional components: the film-forming agent needs to be uniformly combined with the drug, warning color and other components to form a dense and stable composite film layer. This not only can regulate the release speed of the drug and prolong the effective period, but also can avoid the fading or premature shedding of the warning color when meeting water, so as to ensure the safety and recognizability of sowing. Therefore, the selection of film-forming agent is a complex system engineering involving material science, plant physiology and preparation technology, which directly determines the final effect and reliability of the seed treatment agent. The current film-forming agent cannot meet the above requirements.

[0005] Polydimethylsiloxane is a high molecular material, which is cured by cross-linking of active groups at the molecular chain end to form a high molecular elastomer film material with a large intermolecular gap. Compared with traditional polyester film, it has 50 times better air permeability, excellent weather resistance and temperature resistance (negative 60-200°C), higher elasticity and resilience, biocompatibility, etc. By special process, it is prepared into a thin film material, which is widely used in cell culture, medical wound dressing film, gene detection and other fields.

[0006] Currently, the prior art does not report the application of polydimethylsiloxane to seed treatment suspending agent film forming agent. SUMMARY

[0007] Invention objectives To solve the problems in the prior art that the pesticide composition containing fluazinam has poor treatment effect, when it is used as an active ingredient for seed treatment suspending agent, the film forming agent is easily shed by the influence of air temperature, and the mutual fusion effect with the pesticide and warning color is poor, which leads to the problems of inability to prolong the efficacy, discoloration, etc., a pesticide composition containing fluazinam, a seed treatment suspending agent, and a preparation method and application thereof are provided. The pesticide composition has excellent synergistic effect.

[0008] When it is used for seed treatment suspending agent, by compounding with the film forming agent composed of a specific proportion of acrylate and polydimethylsiloxane, it can ensure that the seed coat film is not easy to fall off, and can be adjusted according to the environmental adaptability at the same time, the film layer is mixed uniformly with the pesticide and pigment, the release time of the effective component of the pesticide is prolonged, the problem of seed coating discoloration is effectively solved, and it has excellent effect in preventing and treating peanut root rot, grub, wheat stem base rot and aphid and other agricultural diseases.

[0009] Solution To achieve the purpose of the present application, in a first aspect, the present application provides a pesticide composition containing fluazinam, wherein the active ingredient thereof is composed of fluazinam, fludioxonil and thiamethoxam. The mass ratio of thiamethoxam to fluazinam and fludioxonil is (6-7):(0.4-0.8):(0.2-0.6), and optionally (6-7):0.6:0.4.

[0010] Further, the dosage form of the pesticide composition is one or several of seed treatment suspending agent, water suspending agent, water dispersible granule, wettable powder, dry suspending agent, microcapsule suspending-suspending agent, granule, emulsion oil, and water emulsion.

[0011] Optionally, the effective component accounts for 8-24% of the weight of the composition, and optionally 10-15%.

[0012] In a second aspect, the present application provides a seed treatment suspension concentrate, comprising the following raw materials in parts by weight: 1-50 parts of a pesticide active ingredient, 1-15 parts of a dispersing agent, 0.1-5 parts of a synergist, 1-10 parts of a film-forming agent, 0.1-5 parts of a thickening agent, 0-10 parts of a warning color, 1-50 parts of a solvent, and other adjuvants acceptable for the pesticide seed treatment suspension concentrate; The pesticide component consists of fluazinam, fludioxonil and thiamethoxam, and optionally the mass ratio of the thiamethoxam to the fluazinam and fludioxonil is (6-7):0.6:0.4. The film-forming agent is selected from one or more of acrylate and polydimethylsiloxane. The synergist is a plant oligopeptide.

[0013] Further, the seed treatment suspension concentrate comprises the following raw materials in parts by weight: 8-24 parts of a pesticide active ingredient, 0.5-3 parts of a synergist, 3-8 parts of a film-forming agent, 3-15 parts of a dispersing agent, 0.5-3 parts of a thickening agent, 3-10 parts of a warning color, 1-50 parts of a solvent, and other adjuvants acceptable for the pesticide seed treatment suspension concentrate.

[0014] Optionally, the seed treatment suspension concentrate comprises the following raw materials in parts by weight: 8-15 parts of a pesticide active ingredient, 0.5-3 parts of a synergist, 3-8 parts of a film-forming agent, 3-15 parts of a dispersing agent, 0.5-3 parts of a thickening agent, 3-10 parts of a warning color, 1-50 parts of a solvent, and other adjuvants acceptable for the pesticide seed treatment suspension concentrate.

[0015] Optionally, the film-forming agent is a combination of acrylate and polydimethylsiloxane.

[0016] In a certain aspect, the mass ratio of the acrylate to the polydimethylsiloxane in the film-forming agent is 19:1-1:2, optionally 19:1 or 9:1-1:2, optionally 19:1 or 9:1-5:1, and optionally 19:1 or 9:1.

[0017] Optionally, the ratio of the sum of the weight of the synergist and the film-forming agent to the weight of the active ingredient is 1:(1-5); preferably, the ratio is 1:(1-3).

[0018] Optionally, the weight percentage of the synergist does not exceed 5% of the total dose of the seed treatment suspension concentrate.

[0019] Optionally, the weight percentage of the film-forming agent does not exceed 10% of the total dose of the seed treatment suspension concentrate.

[0020] Further, the thickening agent is selected from one or more of magnesium aluminum silicate, white carbon black, and xanthan gum; and optionally, the thickening agent is a combination of magnesium aluminum silicate, white carbon black, and xanthan gum.

[0021] Optionally, the dispersing agent is selected from at least one of nonylphenol polyoxyethylene ether, sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, sodium stearate, sodium lignosulfonate, methyl amyl alcohol and glycerol monostearate, sodium polycarboxylate and sodium triphenyl ethylene phenol polyoxyethylene ether phosphate ester; preferably a combination of sodium polycarboxylate and sodium triphenyl ethylene phenol polyoxyethylene ether phosphate ester.

[0022] Optionally, the warning color is selected from one or more of fast red FR, rose bengal, capsicum red, carmine, congo red, and brilliant blue; optionally, the warning color is fast red F2R.

[0023] Optionally, the solvent is water; optionally, the solvent is deionized water.

[0024] Optionally, the other adjuvants acceptable to the pesticide seed treatment suspension include one or more of antifoaming agents, preservatives, and antifreezing agents.

[0025] In one aspect, the antifoaming agent is silicone; for example, silicone 1860.

[0026] In one aspect, the preservative is sodium benzoate.

[0027] In one aspect, the antifreezing agent is ethylene glycol.

[0028] Optionally, the other adjuvants acceptable to the pesticide seed treatment suspension include the following ingredients in parts by weight: 0-1% antifoaming agent, 0-0.2% preservative, and 0-10% antifreezing agent.

[0029] Preferably, the other adjuvants acceptable to the pesticide seed treatment suspension include the following ingredients in parts by weight: 0.1-1% antifoaming agent, 0.01-0.2% preservative, and 2-10% antifreezing agent.

[0030] Further, the raw materials include the following ingredients in parts by weight: 8-24% pesticide ingredient, 0.5-3% synergist, 3-8% film former, 1-15% dispersing agent, 0.5-3% thickening agent, 3-10% warning color, 5-30% other adjuvants, and the balance water.

[0031] Optionally, the seed treatment suspension includes the following ingredients in parts by weight: 8-24% pesticide ingredient (e.g., 12%, 14%), 0.5-3% synergist (e.g., 1%), 3-8% film former (e.g., 6%), 0.5-3% thickening agent (e.g., 1.22%), 3-10% warning color (e.g., 3%), 3-15% dispersing agent (e.g., 7%), 0.1-1% antifoaming agent (e.g., 0.5%), 0.01-0.2% preservative (e.g., 0.1%), 2-10% antifreezing agent (e.g., 3%), and the balance water.

[0032] Preferably, the raw materials include the following weight percentages: 5-15% thiamethoxam, 1-5% fluazinam, 1-3% fludioxonil, 1-5% sodium styrylphenol polyoxyethylene ether phosphate, 3-5% sodium carboxylate, 0.5-2% plant oligopeptides, 2-6% acrylate, 0.3-4% polydimethylsiloxane, 0.5-1% magnesium aluminum silicate, 0.5-1% silica, 0.5-1% xanthan gum, 0.1-1% organosilicon 1860, 3-10% permanent red F2R, 0.01-0.2% sodium benzoate, and 2-10% ethylene glycol.

[0033] One formulation includes the following raw materials by weight: 7-12% thiamethoxam (e.g., 10.5%, 12%), 0.5-1.2% fluazinam (e.g., 0.9%, 1.2%), 0.5-1% fludioxonil (e.g., 0.6%, 0.8%), 1-5% sodium styrylphenol polyoxyethylene ether phosphate (e.g., 2%), 1-5% sodium carboxylate (e.g., 5%), 0.5-3% plant oligopeptides (e.g., 1%), and 2-6% acrylates (e.g., 5.7%, 5.6%, 5.4%, 5%, 4%, 2%, or any two of these values). The range includes: 0.1-6% polydimethylsiloxane (e.g., 0.3%, 0.4%, 0.6%, 1%, 2%, 4% or any combination of two values), 0.5-1% magnesium aluminum silicate (e.g., 0.5%), 0.5-1% silica (e.g., 0.6%), 0.5-1% xanthan gum (e.g., 0.12%), 0.1-1% organosilicon 1860 (e.g., 0.5%), 3-10% permanent red F2R (e.g., 3%), 0.01-0.2% sodium benzoate (e.g., 0.1%), and 2-10% ethylene glycol (e.g., 3%).

[0034] The content mentioned is a percentage by mass.

[0035] Thirdly, the present invention provides a method for preparing the seed treatment suspension as described in the second aspect, comprising the following steps: S1: Mix and shear pesticide components, synergists, thickeners, and other adjuvants acceptable to pesticide seed treatment suspensions to obtain coarse slurry; S2: Mix the coarse slurry with thickener, film-forming agent and warning color evenly to obtain seed treatment suspension.

[0036] Optionally, the pesticide ingredients, the defoamer, the dispersant, the thickener, the film-forming agent, the warning color, and the water are as described above.

[0037] Optionally, the mixing temperature is 5~40°C.

[0038] Optionally, the mixing time is 5-20 minutes, preferably 10-20 minutes, for example 15 minutes.

[0039] Optionally, the coarse slurry needs to be sand-milled before the mixing and shearing, preferably at a speed of 1000-2000 rpm, for example at 1800 rpm.

[0040] In one embodiment, the grinding process is based on a particle size D90 ≤ 6.0 μm.

[0041] In one embodiment, the coarse slurry is prepared by shearing with a shearing machine, wherein the shearing time is preferably 1-10 minutes, for example, 5 minutes.

[0042] Fourthly, the present invention provides the application of the pesticide composition as described in the first aspect, the seed treatment suspension as described in the second aspect, or the seed treatment suspension prepared by the preparation method described in the third aspect in the prevention and control of agricultural diseases.

[0043] In one embodiment, the agricultural diseases are peanut root rot, grubs, and wheat stem base rot or aphids.

[0044] Advantages (1) In the embodiments of the present invention, a pesticide composition composed of specific fluazinam, fludioxonil and thiamethoxam exhibits a significant synergistic effect against Fusarium graminearum.

[0045] (2) In the embodiments of the present invention, the pesticide composition consisting of fluazinam, fludioxonil and thiamethoxam is applied in the seed treatment suspension. By combining it with a film-forming agent consisting of acrylate and polydimethylsiloxane, the pesticide solution can be evenly distributed on the seed surface to form a film of uniform thickness, which has the effects of low shedding rate (low shedding rate to below 5%) and not easy to fade.

[0046] (3) The seed treatment suspension of the present invention, through the synergistic effect of adding specific synergists and film-forming agents, increases the root system and tillering of peanuts and wheat, resulting in a significant increase in yield. The seed germination rate can reach 90% or more.

[0047] (4) The seed treatment suspension of the present invention mixes more uniformly with the pesticide components, which can effectively prolong the release time of the active ingredients of the pesticide, thereby prolonging the pesticide's residual effect, effectively controlling the damage of peanuts and wheat to pests and diseases, and increasing crop yield. Detailed Implementation

[0048] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Unless otherwise expressly stated, throughout the specification and claims, the term "comprising" or its variations such as "including" or "comprising of," etc., will be understood to include the stated elements or components, and does not exclude other elements or other components.

[0049] Furthermore, to better illustrate the present invention, numerous specific details are provided in the following detailed embodiments. Those skilled in the art should understand that the present invention can be practiced without certain specific details. In some embodiments, materials, elements, methods, and means well known to those skilled in the art are not described in detail in order to highlight the spirit of the invention.

[0050] 1. Indoor activity test Test Basis: The test was conducted in accordance with NY / T 1156.2-2006 "Guidelines for Indoor Bioassay Testing of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Mycelial Growth of Pathogenic Fungi". Test reagents: 95% fluazinam technical grade, purchased from Zhejiang Heben Technology Co., Ltd.; 98% fludioxonil technical grade, purchased from Zhejiang Yulong Pharmaceutical Co., Ltd.; thiamethoxam technical grade, purchased from Jiangsu Zhongqi Technology Co., Ltd. Test strain: The strain used in this experiment was *Fusarium graminearum*, which was collected by the applicant in 2022 from wheat plants infected with wheat stem rot in Shangqiu, Henan Province. After isolation, purification, and identification, it was stored in the laboratory. Before the experiment, the strain was activated and stored in a freezer at 4°C for later use. The culture medium used was potato dextrose agar (PDA) (this *Fusarium graminearum* is a common type of *Fusarium graminearum*, which can be isolated or commercially available).

[0051] Other reagents: acetone (analytical grade), Tween 80 (analytical grade).

[0052] Drug preparation: Under aseptic conditions, the technical grade fluazinam and fludioxonil were dissolved in acetone to prepare a 10 g / L stock solution. Then, fluazinam and fludioxonil were mixed in mass ratios of 8:2, 7:3, 6:4, 5:5, 4:6, and 3:7 to prepare 6 groups of mixed solutions. Then, 1 ml each of the fluazinam stock solution, fludioxonil stock solution, and the above 6 mixed solutions were added to 9 ml of PDA medium to prepare drug-containing plates of different concentrations. Each treatment consisted of 3 plates, with 3 replicates, and was designated as the treatment group. The blank control group was prepared by mixing 1 ml of acetone solvent with 9 ml of PDA medium. Finally, the cultured *Fusarium graminearum* strain was used to make mycelial cakes with a diameter of 6 mm using a sterile punch. The mycelial cakes were then transferred to the center of the drug-containing PDA plates (mycelial side down) and incubated at 24°C for 3 days.

[0053] Experimental investigation: When the colony diameter of the blank control reaches about 90% of the diameter of the petri dish, the colony diameter is measured using the cross-cross method, and the mycelial growth inhibition rate is calculated.

[0054] Inhibition rate calculation formula: (1) Inhibition rate (%) = (Coronavirus diameter of control group - Coronavirus diameter of treatment group) / (Coronavirus diameter of control group - Diameter of mycelial cake) × 100 The co-toxicity coefficient (CTC) of the indoor activity test data was calculated using the Sun Yunpei method, and the effects of mixed use were evaluated. (Fluorazide was used as the standard reagent, with a toxicity index of 100.) Toxicity index TI(B) = EC of standard reagent A 50 EC of Agent B 50 ×100 Actual toxicity index ATI (AB) = EC of A 50 ÷AB of EC 50 ×100 Theoretical toxicity index TTI(AB) = TI(A) × percentage of A in the mixture + TI(B) × percentage of B in the mixture Actual Toxicity Index (ATI) = (Standard reagent EC) 50 ÷ Test reagent EC 50 )×100 Theoretical Toxicity Index (TTI) = Toxicity Index of Agent A × Percentage of A in the Mixture + Toxicity Index of Agent B × Percentage of B in the Mixture Co-toxicity coefficient (CTC) = [Actual toxicity index (ATI) of the mixture ÷ Theoretical toxicity index (TTI) of the mixture] × 100 The evaluation criteria are as follows: a co-toxicity coefficient ≥ 120 indicates a synergistic effect; a co-toxicity coefficient ≤ 80 indicates an antagonistic effect; and 80 < co-toxicity coefficient < 120 indicates an additive effect.

[0055] The results are shown in Table 1.

[0056] Table 1. Virulence assay results of fluazinam and fludioxonil combined with Fusarium graminearum.

[0057] As shown in Table 1 of the experimental results, the co-toxicity coefficient of fluazinam and fludioxonil in the ratio range of 8:2-4:6 against wheat stem rot is above 120, demonstrating a significant synergistic effect. In particular, the co-toxicity coefficient of the 6:4 ratio reaches over 150, showing an even more significant synergistic effect.

[0058] 2. Toxicity effect test of the three-component mixture The optimal ratio of fluazinam to fludioxonil was determined to be 6:4 based on the above-mentioned indoor activity tests, with a co-toxicity coefficient of 178.43. Furthermore, the 6:4 mixture of fluazinam and fludioxonil (denoted as component C) was used as a fungicide composition and mixed with thiamethoxam (component D) at different mass ratios. The toxicity of the ternary mixture was evaluated using the same testing method described in section 1.2 above, and the results are shown in Table 2.

[0059] Table 2. Virulence assay results of thiamethoxam mixed with fluazinam + fludioxonil (6:4) against Fusarium graminearum.

[0060] The above experiments showed that thiamethoxam alone had no significant activity against Fusarium graminearum. However, when thiamethoxam was prepared into a ternary mixed fungicide with fluazinam and fludioxonil (6:4) in a mass ratio of 7:1 (i.e., the ratio of the three components thiamethoxam: fluazinam: fludioxonil was 7:0.6:0.4), it showed a significant synergistic effect against Fusarium graminearum, with a co-toxicity coefficient (CTC) of 130.19.

[0061] The seed treatment suspension is prepared as follows in the following examples: (1) Add half of the defoamer, dispersant, antifreeze and 20% of the total amount of deionized water to the container in proportion. After stirring evenly, add fluazinam powder, fludioxonil powder, thiamethoxam powder, synergist and thickener (silica and magnesium aluminum silicate), stir for 2 minutes, and shear for 5 minutes to obtain a viscous and easy-to-flow coarse slurry.

[0062] (2) The coarse slurry prepared in step (1) is sand-milled at a speed of 1800 rpm, and the particle size D90≤6.0μm is controlled. After feeding, the remaining defoamer (0.25%), thickener (xanthan gum curing mother liquor) are added, followed by film-forming agent and warning color paste. Stir for about 15 minutes until the thickener is evenly dispersed to obtain the sample, which is a red viscous and easily flowing liquid.

[0063] Unless otherwise stated, all raw materials used in this invention are commercially available.

[0064] Plant oligopeptide adjuvant, purchased from Jiaxing Zhuoan Biotechnology Co., Ltd., product model: ZA-100-1P.

[0065] Sodium polycarboxylate, CAS No. 62601-60-9, was purchased from Jiangsu Qingyu Chemical Technology Co., Ltd.; polydimethylsiloxane, CAS No. 9006-65-9, was purchased from Guangzhou Xike Chemical Technology Co., Ltd.

[0066] Example 1 Thiamethoxam 10.5%, Fluazinam 0.9%, Fludioxonil 0.6%, Sodium Tristyrylphenol Polyoxyethylene Ether Phosphate (Dispersant) 2%, Sodium Polycarboxylate (Dispersant) 5%, Plant Oligopeptide (Synthetic Agent) 1%, Acrylate (Film-forming Agent) 5.7%, Polydimethylsiloxane (Film-forming Agent) 0.3%, Magnesium Aluminum Silicate (Thickener) 0.5%, Silica (Thickener) 0.6%, Xanthan Gum (Thickener) 0.12%, Ethylene Glycol (Antifreeze Agent) 3%, Sodium Benzoate (Preservative) 0.1%, Organosilicon 1860 (Defoamer) 0.5%, Permanent Red F2R (Warning Color) 3%, Water to make up to 100%.

[0067] Example 2 Thiamethoxam 10.5%, Fluazinam 0.9%, Fludioxonil 0.6%, Sodium Tristyrylphenol Polyoxyethylene Ether Phosphate (Dispersant) 2%, Sodium Polycarboxylate (Dispersant) 5%, Plant Oligopeptide (Synthetic Agent) 1%, Acrylate (Film-forming Agent) 5.4%, Polydimethylsiloxane (Film-forming Agent) 0.6%, Magnesium Aluminum Silicate (Thickener) 0.5%, Silica (Thickener) 0.6%, Xanthan Gum (Thickener) 0.12%, Ethylene Glycol (Antifreeze Agent) 3%, Sodium Benzoate (Preservative) 0.1%, Organosilicon 1860 (Defoamer) 0.5%, Permanent Red F2R (Warning Color) 3%, Water to make up to 100%.

[0068] Example 3 Thiamethoxam 10.5%, Fluazinam 0.9%, Fludioxonil 0.6%, Sodium Tristyrylphenol Polyoxyethylene Ether Phosphate (Dispersant) 2%, Sodium Polycarboxylate (Dispersant) 5%, Plant Oligopeptide (Synthetic Agent) 1%, Acrylate (Film-forming Agent) 5%, Polydimethylsiloxane (Film-forming Agent) 1%, Magnesium Aluminum Silicate (Thickener) 0.5%, Silica (Thickener) 0.6%, Xanthan Gum (Thickener) 0.12%, Ethylene Glycol (Antifreeze Agent) 3%, Sodium Benzoate (Preservative) 0.1%, Organosilicon 1860 (Defoamer) 0.5%, Permanent Red F2R (Warning Color) 3%, Water to make up to 100%.

[0069] Example 4 Thiamethoxam 10.5%, Fluazinam 0.9%, Fludioxonil 0.6%, Sodium Tristyrylphenol Polyoxyethylene Ether Phosphate (Dispersant) 2%, Sodium Polycarboxylate (Dispersant) 5%, Plant Oligopeptide (Synthetic Agent) 1%, Acrylate (Film-forming Agent) 4%, Polydimethylsiloxane (Film-forming Agent) 2%, Magnesium Aluminum Silicate (Thickener) 0.5%, Silica (Thickener) 0.6%, Xanthan Gum (Thickener) 0.12%, Ethylene Glycol (Antifreeze Agent) 3%, Sodium Benzoate (Preservative) 0.1%, Organosilicon 1860 (Defoamer) 0.5%, Permanent Red F2R (Warning Color) 3%, Water to make up to 100%.

[0070] Example 5 Thiamethoxam 10.5%, Fluazinam 0.9%, Fludioxonil 0.6%, Sodium Tristyrylphenol Polyoxyethylene Ether Phosphate (Dispersant) 2%, Sodium Polycarboxylate (Dispersant) 5%, Plant Oligopeptide (Synthetic Agent) 1%, Acrylate (Film-forming Agent) 2%, Polydimethylsiloxane (Film-forming Agent) 4%, Magnesium Aluminum Silicate (Thickener) 0.5%, Silica (Thickener) 0.6%, Xanthan Gum (Thickener) 0.12%, Ethylene Glycol (Antifreeze Agent) 3%, Sodium Benzoate (Preservative) 0.1%, Organosilicon 1860 (Defoamer) 0.5%, Permanent Red F2R (Warning Color) 3%, Water to make up to 100%.

[0071] Example 6 Thiamethoxam 10.5%, Fluazinam 0.9%, Fludioxonil 0.6%, Sodium Tristyrylphenol Polyoxyethylene Ether Phosphate (Dispersant) 2%, Sodium Polycarboxylate (Dispersant) 5%, Plant Oligopeptide (Synthetic Agent) 1%, Acrylate (Film-forming Agent) 5.6%, Polydimethylsiloxane (Film-forming Agent) 0.4%, Magnesium Aluminum Silicate (Thickener) 0.5%, Silica (Thickener) 0.6%, Xanthan Gum (Thickener) 0.12%, Ethylene Glycol (Antifreeze Agent) 3%, Sodium Benzoate (Preservative) 0.1%, Organosilicon 1860 (Defoamer) 0.5%, Permanent Red F2R (Warning Color) 3%, Water to make up to 100%.

[0072] Example 7 Thiamethoxam 12%, Fluazinam 1.2%, Fludioxonil 0.8%, Sodium tristyrene-phenol polyoxyethylene ether phosphate (dispersant) 2%, Sodium polycarboxylate (dispersant) 5%, Plant oligopeptide (synergist) 1%, Acrylate (film-forming agent) 5%, Polydimethylsiloxane (film-forming agent) 1%, Magnesium aluminum silicate (thickener) 0.5%, Silica (thickener) 0.6%, Xanthan gum (thickener) 0.12%, Ethylene glycol (antifreeze agent) 3%, Sodium benzoate (preservative) 0.1%, Organosilicon 1860 (defoamer) 0.5%, Permanent Red F2R (warning color) 3%, Water to make up to 100%.

[0073] Comparative Example 1.1 Thiamethoxam 10.5%, Fluazinam 0.9%, Fludioxonil 0.6%, Sodium Tristyrylphenol Polyoxyethylene Ether Phosphate (Dispersant) 2%, Sodium Polycarboxylate (Dispersant) 5%, Plant Oligopeptide (Synthetic Agent) 1%, Acrylate (Film Forming Agent) 6%, Magnesium Aluminum Silicate (Thickener) 0.5%, Silica (Thickener) 0.6%, Xanthan Gum (Thickener) 0.12%, Ethylene Glycol (Antifreeze Agent) 3%, Sodium Benzoate (Preservative) 0.1%, Organosilicon 1860 (Defoamer) 0.5%, Permanent Red F2R (Warning Color) 3%, Water to make up to 100%.

[0074] Comparative Example 1.2 Thiamethoxam 10.5%, Fluazinam 0.9%, Fludioxonil 0.6%, Sodium Tristyrylphenol Polyoxyethylene Ether Phosphate (Dispersant) 2%, Sodium Polycarboxylate (Dispersant) 5%, Plant Oligopeptide (Synthetic Agent) 1%, Polydimethylsiloxane (Film Forming Agent) 6%, Magnesium Aluminum Silicate (Thickener) 0.5%, Silica (Thickener) 0.6%, Xanthan Gum (Thickener) 0.12%, Ethylene Glycol (Antifreeze Agent) 3%, Sodium Benzoate (Preservative) 0.1%, Organosilicon 1860 (Defoamer) 0.5%, Permanent Red F2R (Warning Color) 3%, Water to make up to 100%.

[0075] Comparative Example 1.3 Thiamethoxam 10.5%, Fluazinam 0.9%, Fludioxonil 0.6%, Sodium Tristyrene-phenol Polyoxyethylene Ether Phosphate (Dispersant) 2%, Sodium Polycarboxylate (Dispersant) 5%, Acrylate (Film-forming Agent) 5.4%, Polydimethylsiloxane (Film-forming Agent) 0.6%, Magnesium Aluminum Silicate (Thickener) 0.5%, Silica (Thickener) 0.6%, Xanthan Gum (Thickener) 0.12%, Ethylene Glycol (Antifreeze Agent) 3%, Sodium Benzoate (Preservative) 0.1%, Organosilicon 1860 (Defoamer) 0.5%, Permanent Red F2R (Warning Color) 3%, Water to make up to 100%.

[0076] In this embodiment of the invention, the percentage content is a mass percentage content.

[0077] Coating steps: Take 1000g of seeds and pour them into a coating machine. Spray the prepared seed treatment suspension onto the seeds and stir for 5-10 minutes until each seed is evenly colored. The mass ratio of seed treatment suspension to seeds is 1:125. After coating, spread the seeds out in a ventilated place and let them stand for 2-4 hours until the film solidifies, thus obtaining the sample coated seeds.

[0078] Test Example 1: Detection of Film-Forming Properties and Suspension Rate Seed water resistance test: 100g of the above-mentioned coated seeds were placed in bottles containing 500ml of deionized water and soaked for 24 hours. The color of the seeds and the turbidity of the water were observed.

[0079] Determination of coating uniformity: The uniformity (X3) of the coated seeds of each sample was determined according to the method of coating uniformity in 4.10 of GB / T17768-1999.

[0080] Adhesion and shedding rate determination: Refer to GB / T 43174-2023 "Method for Determination of Adhesion of Pesticide Seed Treatment Formulations" and GB / T17768-1999 "Determination of Coating Shedding Rate".

[0081] Coating shedding rate (%) = (A0×m1-A1×m0)×100 / (A0×m1), and the calculation is repeated 3 times and the average value is taken.

[0082] Where: m0—the mass of the coated seeds used to prepare solution A; m1—The mass of the coated seeds taken for the preparation of solution B; A0—Absorbance of solution A; A1—Absorbance of solution B.

[0083] The results are shown in Table 3.

[0084] Table 3 Results of various test indicators for seed treatment suspension

[0085] As shown in Table 3, the seed treatment suspension of the present invention uses a composite coating of acrylate and polydimethylsiloxane. After the seeds are soaked in water for 15 days, no fading occurs, the water is not turbid, and the coating peeling rate is less than 5%.

[0086] Test Example 2: Safety test and field efficacy trial of seed treatment suspension on peanut seeds. 2.1 Experimental Site: A peanut field in Zhumadian, Henan Province. The soil is sandy, with good aeration and moderate fertility. Peanuts have been grown in the experimental field year-round, and root rot and grub infestations have been common problems in previous years. The peanut variety planted during the experiment was Yuhua 37. 2.2 Test reagents: Reagents prepared in Examples 1-7 and Comparative Examples 1.1-1.3 The control agent CK1 was a 9% thiamethoxam·fludioxonil·methoxam seed treatment suspension, with a ratio of thiamethoxam:fludioxonil:methoxam = 7.5:0.5:1. The registration certificate number was PD20243258, and the manufacturer was Dengfeng Jinbo Pesticide Chemical Co., Ltd. It was purchased from the market.

[0087] The control agent CK2 is a 7.5% pyraclostrobin·flufenican·thiamethoxam seed treatment suspension, in which the ratio of pyraclostrobin:flufenican:thiamethoxam = 1:0.5:6. Registration certificate number: PD20220332. Manufacturer: Shandong United Pesticide Industry Co., Ltd. Purchased from the market.

[0088] The blanks are from the same batch of uncoated naked seeds. 2.3 Test Method: First, dilute the weighed pesticides with water (pesticide to water ratio of 1:3). Then, slowly pour the solution onto 15 kg of prepared peanut seeds, stirring constantly to ensure even distribution of the solution on each seed and coat it completely. After drying, sow the seeds the following day (June 10, 2024). Sow manually, 2 seeds per hole, with a row spacing of 25-30 cm and a plant spacing of 18 cm. After sowing, cover with soil and then apply a pre-emergence herbicide to control weeds.

[0089] 2.4 Test Design: There are 11 treatments, each with 4 replicates, for a total of 44 cells. Each cell consists of 2 rows and 1 row is an isolation zone. The cells are arranged in a randomized block design.

[0090] 2.5 Survey Methods 2.5.1 Emergence status: On June 25, 2024 (15 days), the number of peanut seedlings in 40 holes of each treatment was investigated and the emergence rate was calculated.

[0091] Germination rate % = (Actual number of germinations / Total number of seeds sown) × 100% 2.5.2 Peanut index determination: During the flowering and pegging period of peanuts (July 15, 2024, 35 days), 5 points were investigated for each treatment, and 10 plants were investigated at each point. The disease severity of each plant was determined according to the severity of disease at the base of the stem and the main root. The peanut disease index and relative control efficacy were then calculated.

[0092] Peanut root rot disease grading standards: Grade 0: No lesions on the base of the stem and the main root; Grade 1: A few lesions are present at the base of the stem and on the main root; Grade 3: Numerous lesions on the base of the stem and the main root, with the lesion area accounting for less than 25% of the total area of ​​the stem and roots; Grade 5: Numerous and large lesions on the stem base and main root, covering 25-50% of the total area of ​​the stem and roots. Grade 7: The lesions on the base of the stem and the main root are confluent, and the lesion area accounts for 50% to 75% of the total area of ​​the stem and roots. Level 9: Root necrosis, withered or dead above-ground parts of the plant.

[0093] Calculation formula: Disease index = [∑(number of diseased plants at each level × relative disease severity value) / (total number of plants surveyed × highest disease severity value)] × 100; Prevention and control efficacy (%) = (Disease index of blank control - Disease index of drug treatment) / Disease index of blank control × 100%; 2.5.3 Survey of grub population density During the peanut harvest, a sampling survey was conducted using a 5-point sampling method, with each point 1 meter apart. 2 Dig to a depth of 30cm, record the number of grubs in the soil, compare with the number of uncoated gymnosperms (blank control), and calculate the insect-repellent effect. Insect control effect % = (Number of live insects in the blank control area - Number of live insects in the pesticide-treated area / Number of live insects in the blank control area) × 100%.

[0094] The results are shown in Tables 4 and 5.

[0095] Table 4. Efficacy of seed treatment suspension in controlling peanut growth and root rot.

[0096] Note: The data for each treatment in the table are the average of 4 repetitions.

[0097] As shown in Table 4 above, the peanut seedling emergence rate, peanut root number, and root rot control efficacy of the seed treatment suspension of the present invention are all higher than those of comparative examples 1.1-1.3 and control agents CK1 and CK2. The number of peanut roots determines the number of pods and yield in the later stage of peanut production. This experiment proves that the seed treatment suspension of the present invention has excellent effects on peanut growth and root rot control.

[0098] Table 5. Effects of seed treatment suspension on insect control efficacy and yield of grubs.

[0099] Note: The data for each treatment in the table are the average of four replicates. As shown in Table 5, the seed treatment suspension of this invention uses a mixture of acrylate and polydimethylsiloxane for coating, and then adds plant oligopeptides as a synergist. This can slow down the release rate of thiamethoxam, significantly reduce the damage caused by grubs in the later stages of peanut growth, increase the fresh weight of peanuts, and significantly increase yield.

[0100] Test Example 3: Field efficacy trial of seed treatment suspension on wheat growth and stem rot. 3.1 Seed water tolerance test: 100g of coated wheat seeds were placed in bottles containing 500ml of deionized water and soaked for 24 hours. The color of the seeds and the turbidity of the water were observed.

[0101] 3.2 Field efficacy trial of pesticides for wheat stem base rot 3.2.1 Experimental Site: A wheat field in Yandian Township, Feixi County, Anhui Province. The experimental site was flat, and the wheat variety planted during the experiment was Zhongmai 578. 3.2.2 Test reagents: Reagents prepared in Examples 1-7 and Comparative Examples 1.1-1.3 Control agent CK3: 33% fludioxonil·thiamethoxam suspension seed coating agent, registration certificate number PD20183391, manufacturer: Xingnong Pharmaceutical (China) Co., Ltd., purchased from the market.

[0102] The blanks are from the same batch of uncoated naked seeds.

[0103] 3.2.3 Seed treatment: Before sowing, the seeds are coated. The seed rate for wheat is 300 kg / hm². 2 First, mix each agent with water to form a slurry according to the required dosage (Table 6) (the mass ratio of agent to water is 1:4). Then, slowly pour the solution into 15 kg of prepared seeds, stirring the seeds while pouring to ensure the solution is evenly distributed on the surface of each seed. Finally, place the seeds in a ventilated place to dry. Sow the seeds on the second day (November 9, 2024).

[0104] 3.2.4 Experimental Design The experiment consisted of 10 treatments, each with 4 replicates, arranged in a randomized block design, with a plot size of 30m². 2 Each plot was designated with a sowing area, clearly marked, measuring 100 cm in length and 40 cm in width, and sown with 200 wheat seeds to facilitate later monitoring of germination rates. Isolation zones were established between plots, and soil, water, and fertilizer management were identical across all treatments.

[0105] 3.2.5 Survey Methods In this experiment, a total of 3 surveys were conducted.

[0106] (1) The first survey was conducted 10 days after wheat sowing. The number of seedlings in each plot of quantitative sowing area was investigated, and the seedling emergence rate was calculated. The second survey was conducted 30 days after sowing. The number of wheat tillers was investigated at each of the 5 points. The number of tillers of 3 wheat plants was investigated at each point, and the average value was taken.

[0107] Germination rate % = (Actual number of germinations / Total number of seeds sown) × 100% (2) The third survey was conducted on the disease index of wheat during the greening and jointing stage (March 16, 2025). The diagonal 5-point sampling method was used, with 20 plants taken from each point area, for a total of 100 wheat plants. The disease rate of wheat during the greening and jointing stage was calculated.

[0108] Calculation formula: Disease index = (number of diseased plants / total number of plants surveyed) × 100%.

[0109] The results are shown in Table 6.

[0110] Table 6. Effects of seed treatment suspension on wheat growth and incidence of wheat stem rot.

[0111] Note: The data for each treatment in the table are the average of 4 repetitions.

[0112] Table 6 shows that uncoated wheat had 2.97 tillers per plant, an emergence rate of 80.25%, and a disease incidence rate of 24.50%. This indicates that seed coating improved the emergence rate and reduced the disease incidence rate. Furthermore, the addition of synergists to the seed treatment agent increased the number of tillers. Coated wheat exhibited better growth than uncoated wheat, primarily characterized by thicker, wider, darker green leaves, upright leaves, and a well-developed root system. Example 2, in particular, demonstrated an exceptionally high emergence rate of 4.42 tillers per plant and the lowest incidence of wheat stem rot. These results are of significant value for the development of control methods for wheat stem rot.

[0113] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A pesticide composition containing fluazinam, characterized in that, Its active ingredients consist of fluazinam, fludioxonil, and thiamethoxam; The mass ratio of thiamethoxam, fluazinam, and fludioxonil is (6~7):(0.4~0.8):(0.2~0.6).

2. The pesticide composition according to claim 1, characterized in that, The mass ratio of thiamethoxam, fluazinam, and fludioxonil is (6~7):0.6:0.4; Optionally, the pesticide composition is formulated as one or more of the following: seed treatment suspension, aqueous suspension, water-dispersible granules, wettable powder, dry suspension, microcapsule suspension-suspension, granules, emulsifiable concentrate, and water-in-oil emulsion. Optionally, the active ingredient accounts for 8-24% of the weight of the composition, and optionally 10-15%.

3. A seed treatment suspension containing fluazinam, characterized in that, The raw materials include the following parts by weight: 1-50 parts of pesticide active ingredient, 0.1-5 parts of synergist, 1-10 parts of film-forming agent, 1-15 parts of dispersant, 0.1-5 parts of thickener, 0-10 parts of warning color, 1-50 parts of solvent, and other adjuvants acceptable to pesticide seed treatment suspensions; The pesticide component is composed of fluazinam, fludioxonil, and thiamethoxam, and optionally the mass ratio of thiamethoxam to fluazinam and fludioxonil is (6~7):0.6:0.4; The film-forming agent is selected from one or more of acrylates and polydimethylsiloxane; The synergist is a plant oligopeptide.

4. The seed treatment suspension as described in claim 3, characterized in that, The raw materials include the following weight fractions: 8-24 parts of pesticide active ingredient, 0.5-3 parts of synergist, 3-8 parts of film-forming agent, 3-15 parts of dispersant, 0.5-3 parts of thickener, 3-10 parts of warning color, 1-50 parts of solvent, and other adjuvants acceptable to pesticide seed treatment suspensions; Optionally, the raw materials include the following parts by weight: 8-15 parts of pesticide active ingredient, 0.5-3 parts of synergist, 3-8 parts of film-forming agent, 3-15 parts of dispersant, 0.5-3 parts of thickener, 3-10 parts of warning color, 1-50 parts of solvent, and other adjuvants acceptable to pesticide seed treatment suspensions; Optionally, the film-forming agent is a combination of acrylate and polydimethylsiloxane; Preferably, in the film-forming agent, the mass ratio of acrylate to polydimethylsiloxane is 19:1-1:2, optionally 19:1 or 9:1-1:2, optionally 19:1 or 9:1-5:1, optionally 19:1 or 9:1; Optionally, the ratio of the sum of the weights of the synergist and the film-forming agent to the weight of the active ingredient is 1:(1-5); preferably, the ratio is 1:(1-3). Optionally, the weight percentage of the synergist shall not exceed 5% of the total dose of the seed treatment suspension; Optionally, the film-forming agent accounts for no more than 10% of the total dose of the seed treatment suspension.

5. The seed treatment suspension according to claim 3 or 4, characterized in that, The thickener is selected from one or more of magnesium aluminum silicate, silica, and xanthan gum; optionally, the thickener is a combination of magnesium aluminum silicate, silica, and xanthan gum. And / or, the dispersant is selected from at least one of nonylphenol polyoxyethylene ether, sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, sodium stearate, sodium lignosulfonate, methylpentanol and glyceryl monostearate, sodium polycarboxylate and sodium tristyrylphenol polyoxyethylene ether phosphate; preferably a combination of sodium polycarboxylate and sodium tristyrylphenol polyoxyethylene ether phosphate. And / or, the warning color is selected from one or more of Permanent Red FR, Rose Essence, Chili Red, Carmine, Congo Red, and Brilliant Blue; optionally, the warning color is Permanent Red F2R; And / or, the solvent is water, and optionally, the solvent is deionized water.

6. The seed treatment suspension agent according to any one of claims 3-5, characterized in that, Other acceptable adjuvants for the pesticide seed treatment suspension include one or more of the following: defoamers, preservatives, and antifreeze agents; Preferably, the defoamer is an organosilicon; for example, organosilicon 1860; Preferably, the preservative is sodium benzoate; Preferably, the antifreeze is ethylene glycol; Optionally, other acceptable adjuvants for the pesticide seed treatment suspension include the following components in parts by weight: 0-1% defoamer, 0-0.2% preservative and 0-10% antifreeze; Preferably, other adjuvants acceptable to the pesticide seed treatment suspension include the following components in parts by weight: 0.1-1% defoamer, 0.01-0.2% preservative, and 2-10% antifreeze.

7. The seed treatment suspension agent according to any one of claims 3-6, characterized in that, The raw materials include the following weight percentages: 8-24% pesticide components, 1-15% dispersant, 0.5-3% synergist, 3-8% film-forming agent, 0.5-3% thickener, 3-10% warning color, 5-30% other adjuvants, and the balance being water; Optionally, the seed treatment suspension contains 8-24% pesticide components, 0.5-3% synergist, and 3-8% film-forming agent; 0.5-3% thickener, 3-10% warning color, 3-15% dispersant, 0.1-1% defoamer, 0.01-0.2% preservative, 2-10% antifreeze and the balance water; The content mentioned is a percentage by mass.

8. The seed treatment suspension according to any one of claims 3-7, characterized in that, The raw materials include the following weight percentages: 5-15% thiamethoxam, 1-5% fluazinam, 1-3% fludioxonil, 1-5% sodium styrylphenol polyoxyethylene ether phosphate, 3-5% sodium carboxylate, 0.5-2% plant oligopeptides, 2-6% acrylate, 0.3-4% polydimethylsiloxane, 0.5-1% magnesium aluminum silicate, 0.5-1% silica, 0.5-1% xanthan gum, 0.1-1% organosilicon 1860, 3-10% permanent red F2R, 0.01-0.2% sodium benzoate, and 2-10% ethylene glycol; Preferably, the raw materials include the following weight percentages: 7-12% thiamethoxam, 0.5-1.2% fluazinam, 0.5-1% fludioxonil, 1-5% sodium styrylphenol polyoxyethylene ether phosphate, 1-5% sodium carboxylate, 0.5-3% plant oligopeptides, 2-6% acrylate, 0.1-6% polydimethylsiloxane, 0.5-1% magnesium aluminum silicate, 0.5-1% silica, 0.5-1% xanthan gum, 0.1-1% organosilicon 1860, 3-10% permanent red F2R, 0.01-0.2% sodium benzoate, and 2-10% ethylene glycol; The content mentioned is a percentage by mass.

9. A method for preparing a seed treatment suspension as described in any one of claims 3-8, characterized in that, Includes the following steps: S1: Mix and shear pesticide components, synergists, thickeners, and other adjuvants acceptable to pesticide seed treatment suspensions to obtain coarse slurry; S2: Mix the coarse slurry with thickener, film-forming agent and warning color evenly to obtain seed treatment suspension.

10. The application of a pesticide composition as described in claim 1 or 2, a seed treatment suspension as described in any one of claims 3-8, or a seed treatment suspension prepared by the preparation method described in claim 9 in the prevention and control of agricultural diseases; Optionally, the agricultural diseases mentioned are peanut root rot, grubs, wheat stem base rot, or aphids.

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