Biological fermentation compound feed as well as preparation process and fermentation equipment thereof

Abstract

The present invention discloses a biological fermentation compound feed and a preparation process and fermentation equipment thereof, and belongs to the technical field of feeds, the feed comprises the following raw material components in parts by mass: 48-50 parts of corn, 12.5-13.5 parts of soybean meal, 7.8-8.2 parts of extruded soybeans, 9.7-10.3 parts of rice meal, 2.8-3.2 parts of soybean protein concentrate, 3.95-4.15 parts of tea seed meal, 0.96-1.04 parts of honeysuckle stem and leaf powder, and 4.85-5.25 parts of mung bean powder. The bacillus coagulans, the yeast fermentation bacterial liquid, the enterococcus faecalis and the bacillus subtilis are matched to generate a rich hydrolase system, cellulose with different plant sources, molecular weights and different carbon chain characteristics is degraded, cell walls of plant tissues are destroyed, and therefore exudation of intracellular nutrient substances such as protein, amino acid and nucleic acid is increased, and the nutrient content of plants is increased. The nutritional degree of the feed is improved, the feed conversion rate is increased, the intestinal flora balance can be promoted, the animal health is promoted, antibiotics can be replaced, and the defects of drug residues and antibiotic resistance are avoided.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of feed, in particular to a biological fermentation compound feed and a preparation process and fermentation equipment thereof. BACKGROUND

[0002] The fermented feed is a biological fermentation feed that uses the action of microorganisms to convert feed raw materials into microbial cell protein, bioactive small peptide amino acids, microorganism active probiotics and compound enzyme preparations. However, in the current feed production process, the addition of the fermented feed causes harm to the health of consumers, and the products are not differentiated and have poor quality, which seriously affects the healthy development of the animal husbandry.

[0003] Meanwhile, a fermentation device for processing pig feed production line is disclosed in Chinese Patent No. CN214270847U, which sets an electric telescopic rod, a connecting block, a movable rod, a lifting ring and an annular scraper. The electric telescopic rod drives the connecting block and the movable rod to move downward, and then the lifting ring drives the annular scraper to move downward, so as to scrape the material on the inner wall of the fermentation cylinder, thereby avoiding the waste of the material. However, when the feed is subjected to aerobic fermentation, the feed is prone to accumulate together due to gravity, which makes it difficult to fully contact with oxygen, thereby affecting the fermentation efficiency of the feed. Moreover, the fermentation liquid is deposited at the bottom of the fermentation tank due to gravity, and the material below is fermented faster and more mature, while the material on the upper layer is slower, which easily causes uneven fermentation of the upper and lower layers.

[0004] Therefore, the present application provides a biological fermentation compound feed and a preparation process and fermentation equipment thereof to solve the above problems. SUMMARY

[0005] In view of the above-mentioned shortcomings of the prior art, the present application provides a biological fermentation compound feed and a preparation process and fermentation equipment thereof.

[0006] To achieve the above object, the present application is implemented by the following technical solutions: A biological fermentation compound feed, the feed comprises the following components by mass fraction: corn 48-50 parts, soybean meal 12.5-13.5 parts, puffed soybean 7.8-8.2 parts, rice flour 9.7-10.3 parts, soybean protein concentrate 2.8-3.2 parts, tea seed meal 3.95-4.15 parts, honeysuckle stem and leaf powder 0.96-1.04 parts, mung bean powder 4.85-5.25 parts, choline chloride 0.96-1.05 parts, sodium chloride 0.23-0.26 parts, calcium hydrogen phosphate 0.77-0.84 parts, stone powder 0.45-0.55 parts, trace element premix 1.95-2.04 parts, compound vitamin 0.03-0.05 parts, lysine phosphate 0.62-0.68 parts, methionine 0.33-0.36 parts, threonine 0.24-0.26 parts, tryptophan 0.09-0.11 parts, compound enzyme 0.28-0.32 parts, bacillus coagulans 2.3-3.8 parts, yeast fermentation bacteria liquid 2-5 parts, enterococcus faecalis 3.5-5.5 parts and bacillus subtilis 4.5-5.5 parts.

[0007] Further, the compound enzyme comprises lipase and alkaline protease, wherein the addition amount of the lipase is 3 u / g, and the addition amount of the alkaline protease is 0.6 u / g.

[0008] Further, the yeast fermentation bacteria liquid is a mixed bacteria liquid of candida utilis and saccharomyces cerevisiae at a mass ratio of 1.2:1.

[0009] A preparation process of a biological fermentation compound feed, comprising the following steps: Step one: the tea seed meal, honeysuckle stem and leaf and mung bean powder are crushed, mixed with water, uniformly mixed and then sterilized; Step two: the pure culture of bacillus coagulans, yeast fermentation bacteria liquid, enterococcus faecalis, bacillus subtilis and compound enzyme is added into the sterilized material, and then the compound vitamin and trace element are added and mixed; Step three: the fermentation device is adjusted to 28-30 DEG C, and then the mixture is added into the fermentation device for aerobic culture for 36-48 h; Step four: after the aerobic stage is finished, the fermented feed is scattered, uniformly mixed, packed into a one-way exhaust sealed bag and placed at room temperature for anaerobic culture for 4-8 d.

[0010] A fermentation device for the preparation process of a biological fermentation compound feed, comprising a fermentation tank, and a stirring assembly for improving mixing degree connected to the top of the fermentation tank; An adjustable feeding assembly for uniform oxygen injection and fermentation liquid circulation is further connected to the top of the fermentation tank, and the adjustable feeding assembly is connected with the stirring assembly; The bottom of the fermentation tank is fixedly connected with an adjustable discharging assembly for filtering the fermentation liquid during fermentation and guiding the discharging during discharging, and the adjustable discharging assembly is connected with the adjustable feeding assembly, and the gas outlet end and the liquid outlet pipe of the adjustable feeding assembly are located at the stirring end of the stirring assembly. The lower end of the side wall of the fermentation tank is connected with a sampling assembly for sampling.

[0011] Further, the stirring assembly comprises a motor, a first bevel gear, a second bevel gear, a hollow shaft and stirring rods, the motor is fixedly installed on the top of the fermentation tank, the first bevel gear is fixedly installed on the driving end of the motor, the first bevel gear is meshingly connected with the second bevel gear, the second bevel gear is fixedly installed on the upper end of the hollow shaft, the top of the fermentation tank is rotationally connected with the hollow shaft through a bearing, a plurality of groups of stirring rods are fixedly installed on the part of the hollow shaft located in the fermentation tank, and the hollow shaft and the stirring rods are connected with the adjustable feeding assembly, and the gas outlet end and the liquid outlet pipe of the adjustable feeding assembly are located at the stirring rods.

[0012] Further, the adjustable discharging assembly comprises a connecting plate, sliding plates, protective covers, a first cylinder, a straight pipe, a gate plate and a filter plate, the straight pipe is fixedly installed on the bottom of the fermentation tank, two groups of protective covers are fixedly installed on the outer wall of the straight pipe in communication, the first cylinder is fixedly connected with the protective covers, the connecting plate is fixedly installed on the driving end of the first cylinder, two groups of sliding plates are fixedly installed on the connecting plate, the two groups of sliding plates are respectively fixedly connected with the gate plate and the filter plate, the gate plate and the filter plate are respectively slidingly connected with the inner wall of the protective covers, the gate plate and the filter plate are respectively slidingly connected with the transverse sliding holes of the straight pipe, the filter plate is located above the gate plate, the adjustable feeding assembly is fixedly connected with the straight pipe in communication, and the connection part of the adjustable feeding assembly and the straight pipe is located between the gate plate and the filter plate. Further, the adjustable feeding assembly comprises an air inlet assembly, a liquid adding assembly, a follow-up type guide assembly and an adjusting assembly, the adjusting assembly is fixedly connected with the fermentation tank, the adjusting assembly, the air inlet assembly and the liquid adding assembly are connected with the hollow shaft, the follow-up type guide assembly is connected with the stirring rods, and the follow-up type guide assembly is connected with the adjusting assembly.

[0013] Further, the liquid adding assembly comprises a liquid pump, a liquid suction pipe and a liquid guide sliding ring, the outer ring of the liquid pump and the liquid guide sliding ring is fixedly connected with the top of the fermentation tank, the inner ring of the liquid guide sliding ring is fixedly connected with the hollow shaft, the liquid guide sliding ring communicates with the hollow shaft, the outer ring of the liquid guide sliding ring is fixedly connected with the output end of the liquid pump through a pipeline, the input end of the liquid pump is fixedly connected with the liquid suction pipe, the liquid suction pipe is fixedly connected with the straight pipe, and the connection part of the liquid suction pipe and the straight pipe is located between the gate plate and the filter plate.

[0014] Further, the air inlet assembly comprises an air inlet pipe and an air guide sliding ring, the outer ring of the air guide sliding ring is fixedly connected with the outer ring of the liquid guide sliding ring, and the inner ring of the air guide sliding ring is fixedly connected with the hollow shaft, and the air guide sliding ring communicates with the hollow shaft.

[0015] Beneficial effects: The present invention utilizes Bacillus coagulans, yeast fermentation liquid, Enterococcus faecalis, and Bacillus subtilis to produce a rich hydrolytic enzyme system, which degrades cellulose from different plant sources, with different molecular weights and different carbon chain characteristics, destroys the cell walls of plant tissues, thereby increasing the exudation of intracellular nutrients such as proteins, amino acids, and nucleic acids, improving the nutritional value of feed, increasing feed conversion rate, promoting intestinal flora balance, promoting animal health, and can replace antibiotics, avoiding the drawbacks of drug residues and antibiotic resistance.

[0016] In this invention, the mixture is added to the fermentation tank through the inlet. When mixing is required, the stirring component rotates to agitate the mixture. When oxygen needs to be added, the adjustable feed component introduces gas, which is then sprayed out from the stirring end of the stirring component, ensuring uniform oxygen addition and guaranteeing the uniformity of aerobic fermentation. When fermentation broth circulation is required, the fermentation broth in the fermentation tank enters the adjustable discharge component. The stirring component rotates to agitate the mixture, and the adjustable feed component draws the fermentation broth from the adjustable discharge component, spraying it out from the stirring end of the stirring component. This ensures the fermentation broth circulates and prevents it from remaining completely at the bottom of the fermentation tank, promoting even fermentation and preventing the bottom material from fermenting better than the top material. Simultaneously, the sampling component facilitates sampling within the fermentation tank, aiding in understanding the fermentation process. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0018] Figure 1 The three-dimensional fermentation equipment for the preparation process of bio-fermented compound feed according to the present invention. Figure 1 ; Figure 2 This is a front view of the fermentation equipment used in the preparation process of a bio-fermented compound feed according to the present invention. Figure 3 This is a left view of the fermentation equipment used in the preparation process of a bio-fermented compound feed according to the present invention. Figure 4 The three-dimensional fermentation equipment for the preparation process of bio-fermented compound feed according to the present invention. Figure 2 ; Figure 5 The three-dimensional fermentation equipment for the preparation process of bio-fermented compound feed according to the present invention. Figure 3 ; Figure 6 For alongFigure 3 A sectional view along the AA direction; Figure 7 For along Figure 3 BB direction sectional view; Figure 8 for Figure 6 Enlarged view of the structure at point C; Figure 9 Explosion of sampling component Figure 1 ; Figure 10 For the sampling component to explode Figure 2 .

[0019] The labels in the diagram represent: 1. Fermentation tank; 2. Sampling assembly; 21. Horizontal cylinder; 22. Horizontal plate; 23. First spring; 24. Locking rod; 25. Arc-shaped limiting block; 26. Horizontal hole; 27. Sampling groove; 28. Sampling rod; 29. ​​C-shaped groove; 210. Connecting rod; 211. Handle; 212. Horizontal movable groove; 213. Discharge pipe; 214. Locking hole; 3. Stirring assembly; 31. Motor; 32. First bevel gear; 33. Second bevel gear; 34. Hollow shaft; 35. Stirring rod; 4. Adjustable discharge assembly; 41. Connecting plate; 42. Slide plate; 43. Protective cover; 44. First cylinder; 45. Straight 46. ​​Pipe; 47. Gate; 5. Filter plate; 6. Adjustable feed assembly; 7. Support frame; 8. Second cylinder; 9. Liquid pump; 10. Liquid extraction pipe; 11. Air inlet pipe; 12. Air guide slip ring; 13. Liquid guide slip ring; 14. Pressure plate; 15. Second spring; 16. Square rod; 17. First piston; 18. Second piston; 19. First channel; 20. Third piston; 21. Second channel; 32. Third spring; 43. Movable horizontal plate; 54. First through hole; 55. Conical seat; 66. Horizontal slide groove; 77. Second through hole; 88. Inclined surface. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0021] The present invention will be further described below with reference to embodiments.

[0022] Example 1 This embodiment provides a bio-fermented compound feed, which comprises the following components by weight: 48 parts corn, 13.1 parts soybean meal, 7.8 parts extruded soybeans, 10.3 parts rice flour, 2.9 parts soybean protein concentrate, 3.95 parts tea seed meal, 0.96 parts honeysuckle stem and leaf powder, 5.25 parts mung bean powder, 0.96 parts choline chloride, 0.23 parts sodium chloride, 0.84 parts dicalcium phosphate, 0.49 parts limestone powder, 1.95 parts trace element premix, 0.04 parts compound vitamins, 0.65 parts lysine phosphate, 0.34 parts methionine, 0.25 parts threonine, 0.09 parts tryptophan, 0.31 parts compound enzyme, 2.6 parts Bacillus coagulans, 3 parts yeast fermentation liquid, 4.1 parts Enterococcus faecalis, and 4.5 parts Bacillus subtilis. The multivitamin contains vitamins A, D3, E, K3, B1, B2, B6, B12, pantothenic acid, niacin, folic acid, and biotin. The multivitamin provides the following per kilogram of feed: Vitamin A 5512 IU, Vitamin D3 2250 IU, Vitamin E 24 mg, Vitamin K3 3 mg, Vitamin B1 2 mg, Vitamin B2 6 mg, Vitamin B6 3 mg, Vitamin B12 24 μg, Pantothenic acid 15 mg, Niacin 14 mg, Folic acid 1.2 mg, and Biotin 150 μg.

[0023] The micronutrient premix contains any of the following: glycine iron complex, ferrous fumarate, ferrous sulfate, zinc lactate, zinc methionine complex, zinc sulfate, manganese methionine complex, manganese sulfate, copper citrate, copper sulfate, basic copper chloride, yeast selenium, sodium selenite, and carrier bran. The micronutrient premix provides the following per kilogram of feed: Fe 100mg, Zn 80mg, Cu 20mg, Mn 25mg, I 0.14mg, Se 0.3mg. The complex enzyme includes lipase and alkaline protease, with lipase added at 3 u / g and alkaline protease added at 0.6 u / g.

[0024] The yeast fermentation broth is a mixed broth of Candida utilis and Saccharomyces cerevisiae in a mass ratio of 1.2:1.

[0025] A process for preparing a bio-fermented compound feed includes the following steps: Step 1: Grind tea seed meal, honeysuckle stems and leaves, and mung bean powder, mix with water, and then sterilize. Step 2: Add pure cultures of Bacillus coagulans, yeast fermentation liquid, Enterococcus faecalis, Bacillus subtilis, and compound enzymes to the sterilized material, then add compound vitamins and trace elements and mix. Step 3: Adjust the fermentation device to 30℃, then add the mixture into the fermentation device and incubate aerobically for 48 hours; Step 4: After the aerobic stage is completed, break up and mix the fermented feed, put it into a one-way venting sealed bag, and place it at room temperature for anaerobic culture for 7 days.

[0026] Example 2 This embodiment provides a bio-fermented compound feed, which comprises the following components by weight: 50 parts corn, 12.5 parts soybean meal, 9.1 parts extruded soybeans, 9.7 parts rice flour, 2.8 parts soybean protein concentrate, 4.15 parts tea seed meal, 1.04 parts honeysuckle stem and leaf powder, 4.85 parts mung bean powder, 1.05 parts choline chloride, 0.25 parts sodium chloride, 0.77 parts dicalcium phosphate, 0.55 parts limestone powder, 2.04 parts trace element premix, 0.03 parts compound vitamins, 0.62 parts lysine phosphate, 0.33 parts methionine, 0.24 parts threonine, 0.11 parts tryptophan, 0.32 parts compound enzyme, 2.3 parts Bacillus coagulans, 2 parts yeast fermentation liquid, 3.5 parts Enterococcus faecalis, and 5.5 parts Bacillus subtilis. The multivitamin contains vitamins A, D3, E, K3, B1, B2, B6, B12, pantothenic acid, niacin, folic acid, and biotin. The multivitamin provides the following per kilogram of feed: Vitamin A 5512 IU, Vitamin D3 2250 IU, Vitamin E 24 mg, Vitamin K3 3 mg, Vitamin B1 2 mg, Vitamin B2 6 mg, Vitamin B6 3 mg, Vitamin B12 24 μg, Pantothenic acid 15 mg, Niacin 14 mg, Folic acid 1.2 mg, and Biotin 150 μg.

[0027] The micronutrient premix contains any of the following: glycine iron complex, ferrous fumarate, ferrous sulfate, zinc lactate, zinc methionine complex, zinc sulfate, manganese methionine complex, manganese sulfate, copper citrate, copper sulfate, basic copper chloride, yeast selenium, sodium selenite, and carrier bran. The micronutrient premix provides the following per kilogram of feed: Fe 100mg, Zn 80mg, Cu 20mg, Mn 25mg, I 0.14mg, Se 0.3mg. The complex enzyme includes lipase and alkaline protease, with lipase added at 3 u / g and alkaline protease added at 0.6 u / g.

[0028] The yeast fermentation broth is a mixed broth of Candida utilis and Saccharomyces cerevisiae in a mass ratio of 1.2:1.

[0029] A process for preparing a bio-fermented compound feed includes the following steps: Step 1: Grind tea seed meal, honeysuckle stems and leaves, and mung bean powder, mix with water, and then sterilize. Step 2: Add pure cultures of Bacillus coagulans, yeast fermentation liquid, Enterococcus faecalis, Bacillus subtilis, and compound enzymes to the sterilized material, then add compound vitamins and trace elements and mix. Step 3: Adjust the fermentation device to 28℃, then add the mixture into the fermentation device and incubate aerobically for 36 hours; Step 4: After the aerobic stage is completed, break up and mix the fermented feed, put it into a one-way venting sealed bag, and place it at room temperature for anaerobic culture for 4 days.

[0030] Example 3 This embodiment provides a bio-fermented compound feed, which comprises the following components by weight: 49 parts corn, 13.5 parts soybean meal, 8.2 parts extruded soybeans, 10.1 parts rice flour, 3.2 parts soybean protein concentrate, 4.02 parts tea seed meal, 0.99 parts honeysuckle stem and leaf powder, 5.11 parts mung bean powder, 1.01 parts choline chloride, 0.26 parts sodium chloride, 0.81 parts dicalcium phosphate, 0.45 parts limestone powder, 2.01 parts trace element premix, 0.05 parts compound vitamins, 0.68 parts lysine phosphate, 0.36 parts methionine, 0.26 parts threonine, 0.1 parts tryptophan, 0.28 parts compound enzyme, 3.8 parts Bacillus coagulans, 5 parts yeast fermentation liquid, 5.5 parts Enterococcus faecalis, and 4.9 parts Bacillus subtilis. The multivitamin contains vitamins A, D3, E, K3, B1, B2, B6, B12, pantothenic acid, niacin, folic acid, and biotin. The multivitamin provides the following per kilogram of feed: Vitamin A 5512 IU, Vitamin D3 2250 IU, Vitamin E 24 mg, Vitamin K3 3 mg, Vitamin B1 2 mg, Vitamin B2 6 mg, Vitamin B6 3 mg, Vitamin B12 24 μg, Pantothenic acid 15 mg, Niacin 14 mg, Folic acid 1.2 mg, and Biotin 150 μg.

[0031] The micronutrient premix contains any of the following: glycine iron complex, ferrous fumarate, ferrous sulfate, zinc lactate, zinc methionine complex, zinc sulfate, manganese methionine complex, manganese sulfate, copper citrate, copper sulfate, basic copper chloride, yeast selenium, sodium selenite, and carrier bran. The micronutrient premix provides the following per kilogram of feed: Fe 100mg, Zn 80mg, Cu 20mg, Mn 25mg, I 0.14mg, Se 0.3mg. The complex enzyme includes lipase and alkaline protease, with lipase added at 3 u / g and alkaline protease added at 0.6 u / g.

[0032] The yeast fermentation broth is a mixed broth of Candida utilis and Saccharomyces cerevisiae in a mass ratio of 1.2:1.

[0033] A process for preparing a bio-fermented compound feed includes the following steps: Step 1: Grind tea seed meal, honeysuckle stems and leaves, and mung bean powder, mix with water, and then sterilize. Step 2: Add pure cultures of Bacillus coagulans, yeast fermentation liquid, Enterococcus faecalis, Bacillus subtilis, and compound enzymes to the sterilized material, then add compound vitamins and trace elements and mix. Step 3: Adjust the fermentation device to 29℃, then add the mixture into the fermentation device and incubate aerobically for 42 hours; Step 4: After the aerobic stage is completed, break up and mix the fermented feed, put it into a one-way venting sealed bag, and place it at room temperature for anaerobic culture for 8 days.

[0034] Experimental process and results One hundred and fourteen 28-day-old weaned piglets with an initial weight of 7.02 ± 0.45 kg were selected. The experiment employed a completely randomized block design, with piglets divided into six treatment groups: control group (unfermented basal diet), Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group (honeysuckle stem and leaf powder removed), and Comparative Example 2 group (mung bean powder removed). The experimental period was 28 days.

[0035] Feeding was conducted four times daily at 08:00, 12:00, 16:00, and 20:00. A principle of small, frequent feedings was adopted, adjusting the amount of feed given each time to ensure that there was always a slight residue in the feed trough, guaranteeing that the piglets received sufficient feed. Feeding and management conditions were kept consistent across all treatment groups. The pigsties were fully enclosed, equipped with heat lamps to maintain a constant temperature, and the environment was well-ventilated. Daily cleaning and disinfection were conducted to maintain cleanliness, hygiene, and dryness.

[0036] At the beginning and end of the experiment, each piglet was weighed after a 12-hour fast. During the experiment, the daily feed input and uneaten feed (uneaten feed + waste), and actual feed intake (feed input - uneaten feed) were accurately recorded per pen. The feed conversion ratio (F / G) was calculated based on average daily gain (ADG) and average daily feed intake (ADFI). A digestion test was conducted from day 25 to day 28 of the experiment. Rectal fecal samples from weaned piglets were collected continuously for 4 days using an endogenous indicator fecal collection method. The homogenized fecal samples were mixed with 10% sulfuric acid and an appropriate amount of toluene for preservation and nitrogen content stabilization. Then, the samples were dried at 65°C until the mass remained constant, pulverized, and sieved through a 40-mesh sieve to determine the content of crude protein (CP), crude fat (EE), dry matter (DM), and crude ash (ASH). At the end of day 28 of the experiment, one piglet from each treatment group with a weight close to the average was selected. After fasting, 10 mL of blood was collected from the anterior vena cava. The sample was allowed to stand for 30 minutes, then centrifuged for 10 minutes (1350×g) to separate the serum. The serum was then stored at -20°C for the detection of serum immune indicators. After blood collection, the piglets were anesthetized by injection of sodium pentobarbital and euthanized by exsanguination through the anterior vena cava. The abdominal cavity was then quickly opened, and jejunal mucosal samples were scraped from sterile glass slides, collected in sterile cryovials, and stored at -20°C for analysis of digestive enzyme (lactase, maltase, and sucrase) activities. Colonic contents were collected for microbial count analysis.

[0037] Experimental data were initially processed using Excel 2016, and analysis of variance (ANOVA) was performed using SAS 9.2 statistical software. Duncan's method was used for multiple comparisons to determine statistical significance, with P < 0.05 as the criterion for significance, and 0.05 ≤ P < 0.10 considered a trend. The experimental results are shown in the table below.

[0038] As shown in Table 1, compared with the control group, the use of Example 1, Example 2, and Example 3 of the present invention significantly reduced the feed conversion ratio (F / G) and improved the feed conversion rate in weaned piglets (P<0.05). Compared with the control group, the use of Example 1, Example 2, and Example 3 of the present invention showed a trend of increasing adventitious growth factor (ADG) (P=0.085).

[0039] Table 1. Effects of fermented feed on growth performance of piglets As shown in Table 2, compared with the control group, the digestibility of DM in weaned piglets using Example 1 of the present invention was significantly improved (P<0.05). Compared with the control group, the digestibility of ASH and CP in weaned piglets using Example 3 of the present invention was significantly improved (P<0.05). Compared with the control group, the digestibility of EE in weaned piglets using Example 1, Example 2, and Example 3 of the present invention was significantly improved (P<0.05). The EE digestibility of piglets in Example 1 and Example 3 was significantly higher than that in Comparative Example 1 (P<0.05).

[0040] Table 2. Effects of fermented feed on nutrient digestibility in piglets As shown in Table 3, compared with the control group, the use of Example 1, Example 2, and Example 3 of this invention significantly increased the jejunal mucosal maltase activity in weaned piglets (P<0.05). Compared with the control group, the jejunal mucosal maltase activity in the other groups of weaned piglets was significantly increased (P<0.05).

[0041] Table 3. Effects of fermented feed on the activity of digestive enzymes in the jejunal mucosa of piglets. As shown in Table 4, compared with the control group, the use of Example 1, Example 2, Example 3, and Comparative Example 2 significantly increased the serum IgA content of weaned piglets (P<0.05). Compared with the control group, the serum IgG content of the other groups of weaned piglets was significantly increased (P<0.05). The serum IgA content of piglets in Example 1 was significantly higher than that in Comparative Example 1 and Comparative Example 2 (P<0.05). The serum IgG content of piglets in Example 1, Example 2, and Example 3 was significantly higher than that in Comparative Example 1 (P<0.05).

[0042] Table 4. Effects of fermented feed on serum immune indicators in piglets (log(copy number / g)) As shown in Table 5, compared with the control group, the number of Bacillus oryzae and Lactobacillus in the coli of weaned piglets in the other groups increased significantly, the number of Escherichia coli decreased significantly (P<0.05), and there was a trend of increasing the number of Bifidobacterium (P=0.071).

[0043] Table 5. Effects of fermented feed on the gut microbiota of piglets This application utilizes Bacillus coagulans, yeast fermentation broth, Enterococcus faecalis, and Bacillus subtilis to produce a rich hydrolytic enzyme system. This system degrades cellulose from different plant sources, with varying molecular weights and carbon chain characteristics, thereby disrupting the cell walls of plant tissues. This increases the exudation of intracellular nutrients such as proteins, amino acids, and nucleic acids, enhancing the nutritional value of the feed, increasing feed conversion rate, promoting intestinal flora balance, and improving animal health. It can replace antibiotics, avoiding the drawbacks of drug residues and antibiotic resistance.

[0044] Example 4 Please refer to the instruction manual appendix. Figures 1-10 A fermentation device for preparing a bio-fermented compound feed includes a fermentation tank 1, and a stirring component 3 for improving mixing is connected to the top of the fermentation tank 1. The top of the fermenter 1 is also connected to an adjustable feed assembly 5 for uniform oxygen injection and fermentation broth circulation, and the adjustable feed assembly 5 is connected to the stirring assembly 3. The bottom of the fermenter 1 is fixedly connected to an adjustable discharge component 4 for filtering the fermentation liquid during fermentation and guiding the material during discharge. The adjustable discharge component 4 is connected to the adjustable feed component 5. The air outlet and liquid outlet pipe of the adjustable feed component 5 are both located at the stirring end of the stirring component 3. A sampling component 2 for sampling is connected to the lower end of the side wall of fermenter 1.

[0045] Fermentation tank 1 is fixedly equipped with a feed inlet (not shown in the figure); The mixture is added to fermenter 1 through the feed inlet. When mixing is required, the stirring component 3 rotates to agitate. When oxygen needs to be added, the regulating feed component 5 introduces gas, which is then sprayed out from the stirring end of the stirring component 3, ensuring uniform oxygen addition and guaranteeing the uniformity of aerobic fermentation. When fermentation broth circulation is required, the fermentation broth in fermenter 1 enters the regulating discharge component 4. The stirring component 3 rotates to agitate, and the regulating feed component 5 draws the fermentation broth from the regulating discharge component 4, spraying it out from the stirring end of the stirring component 3. This ensures the fermentation broth circulates and prevents it from remaining completely at the bottom of fermenter 1, promoting even fermentation and preventing the bottom material from fermenting better than the top material. Simultaneously, the sampling component 2 facilitates sampling within fermenter 1, providing insights into the fermentation process.

[0046] The stirring assembly 3 includes a motor 31, a first bevel gear 32, a second bevel gear 33, a hollow shaft 34, and stirring rods 35. The motor 31 is fixedly installed on the top of the fermenter 1. The first bevel gear 32 is fixedly installed on the drive end of the motor 31. The first bevel gear 32 and the second bevel gear 33 are meshed and connected. The second bevel gear 33 is fixedly installed on the upper end of the hollow shaft 34. The top of the fermenter 1 is rotatably connected to the hollow shaft 34 through a bearing. Multiple sets of stirring rods 35 are arrayed and fixedly installed on the part of the hollow shaft 34 located inside the fermenter 1. Both the hollow shaft 34 and the stirring rods 35 are connected to the adjustable feed assembly 5. The air outlet and liquid outlet of the adjustable feed assembly 5 are located on the stirring rods 35. The stirring rods 35 are divided into multiple layers at equal intervals, and each layer of stirring rods 35 is fixedly installed on the hollow shaft 34 at equal intervals along the circumference. When mixing is required, the motor 31 of the stirring assembly 3 drives the first bevel gear 32 to rotate, the first bevel gear 32 drives the second bevel gear 33 to rotate, the second bevel gear 33 drives the hollow shaft 34 to rotate, and the hollow shaft 34 drives the stirring rod 35 to rotate. The stirring rod 35 rotates to stir. When oxygen needs to be added, the adjustable feed assembly 5 introduces gas, which is sprayed out from the stirring rod 35, so that the rotation can evenly add oxygen, which helps to ensure the uniformity of aerobic fermentation. When the fermentation liquid needs to be circulated, the fermentation liquid in the fermentation tank 1 enters the adjustable discharge assembly 4. The stirring rod 35 rotates to stir, and the adjustable feed assembly 5 draws the fermentation liquid in the adjustable discharge assembly 4 and sprays it out from the stirring rod 35, so as to circulate the fermentation liquid and prevent the fermentation liquid from being completely retained at the bottom of the fermentation tank 1. This helps to promote the even fermentation of the material in the fermentation tank 1 and prevents the material at the bottom from fermenting better than the material at the top.

[0047] The adjustable discharge assembly 4 includes a connecting plate 41, a sliding plate 42, a protective cover 43, a first cylinder 44, a straight pipe 45, a gate 46, and a filter plate 47. The straight pipe 45 is fixedly installed at the bottom of the fermenter 1. Two sets of protective covers 43 are fixedly installed on the outer wall of the straight pipe 45. The first cylinder 44 is fixedly connected to the protective cover 43. The connecting plate 41 is fixedly installed on the drive end of the first cylinder 44. Two sets of sliding plates 42 are fixedly installed on the connecting plate 41. The two sets of sliding plates 42 are fixedly connected to the gate 46 and the filter plate 47 respectively. The gate 46 and the filter plate 47 are slidably connected to the inner wall of the protective cover 43 respectively. The gate 46 and the filter plate 47 are slidably connected to the transverse sliding hole opened in the straight pipe 45. The filter plate 47 is located above the gate 46. The adjustable feed assembly 5 is fixedly connected to the straight pipe 45. The connection between the adjustable feed assembly 5 and the straight pipe 45 is located between the gate 46 and the filter plate 47. When the fermentation broth is circulating, the first cylinder 44 of the adjustable discharge assembly 4 drives the connecting plate 41 to move towards the straight pipe 45. The connecting plate 41 drives the slide plate 42 to move towards the straight pipe 45. The connecting plate 41 drives the filter plate 47 and the gate 46 to move into the straight pipe 45. The filter plate 47 performs filtration, and the gate 46 blocks the filtered fermentation broth. Then the adjustable feed assembly 5 extracts it again. When discharge is required, the first cylinder 44 of the adjustable discharge assembly 4 drives the connecting plate 41 to move away from the straight pipe 45. The connecting plate 41 drives the slide plate 42 to move away from the straight pipe 45. The connecting plate 41 drives the filter plate 47 and the gate 46 to move into the protective cover 43, so that the straight pipe 45 is fully open. The fermented material in the fermentation tank 1 is discharged out through the straight pipe 45. The adjustable feeding assembly 5 includes an air inlet assembly, a liquid addition assembly, a follow-up conductive assembly, and an adjustment assembly. The adjustment assembly is fixedly connected to the fermenter 1. The adjustment assembly, air inlet assembly, and liquid addition assembly are connected to the hollow shaft 34. The follow-up conductive assembly is connected to the stirring rod 35. The follow-up conductive assembly and the adjustment assembly are connected. The liquid addition assembly includes a liquid pump 53, a liquid extraction pipe 54, and a liquid guide slip ring 57. The outer rings of the liquid pump 53 and the liquid guide slip ring 57 are fixedly connected to the top of the fermenter 1. The inner ring of the liquid guide slip ring 57 is fixedly connected to the hollow shaft 34 and communicates with the hollow shaft 34. The outer ring of the liquid guide slip ring 57 is fixedly connected to the output end of the liquid pump 53 through a pipe. The input end of the liquid pump 53 is fixedly connected to the liquid extraction pipe 54. The liquid extraction pipe 54 is fixedly connected to the straight pipe 45, and the connection between the liquid extraction pipe 54 and the straight pipe 45 is located between the gate plate 46 and the filter plate 47. The air intake assembly includes an air intake pipe 55 and an air guide slip ring 56. The outer ring of the air guide slip ring 56 is fixedly connected to the outer ring of the liquid guide slip ring 57, and the inner ring of the air guide slip ring 56 is fixedly connected to the hollow shaft 34. The air guide slip ring 56 communicates with the hollow shaft 34. The air inlet pipe 55 is connected to an external air pump; The adjustment assembly includes a support frame 51, a second cylinder 52, a pressure plate 58, a second spring 59, a square rod 510, a first piston 511, a second piston 512, a third piston 514, and a conical seat 519. The support frame 51 is fixedly installed on the top of the fermenter 1, and the second cylinder 52 is fixedly installed on the top of the support frame 51. The pressure plate 58 is rotatably connected to the drive end of the second cylinder 52 via bearings. The upper and lower ends of the second spring 59 are fixedly connected to the top of the square rod 510 and the top of the hollow shaft 34. The top of the hollow shaft 34 is slidably connected to the square rod 510 through a square hole, and the pressure plate 58 is located on the top of the square rod 510. The first piston 511, the second piston 512, and the third piston 514 are also included. Fixedly installed on the square rod 510, the gas guide slip ring 56 is located between the first piston 511 and the second piston 512, and the liquid guide slip ring 57 is located between the second piston 512 and the third piston 514. The third piston 514 is always lower than the bottom of the uppermost stirring rod 35. The square rod 510 has a first channel 513. The upper end of the first channel 513 is located between the first piston 511 and the second piston 512, and the lower end of the first channel 513 is located below the third piston 514. Multiple sets of conical seats 519 are fixedly installed on the square rod 510, and the multiple sets of conical seats 519 are arranged one-to-one with the stirring rod 35 of each layer. Each set of conical seats 519 is movably connected to the follower-type conductive assembly of each layer. The follow-up conductive assembly includes a third spring 516 and a movable horizontal plate 517. A horizontal sliding groove 520 is opened in the stirring rod 35. The end of the horizontal sliding groove 520 away from the hollow shaft 34 is fixedly connected to one end of the third spring 516. The other end of the third spring 516 is fixedly connected to the movable horizontal plate 517. The movable horizontal plate 517 is slidably connected to the inner wall of the horizontal sliding groove 520. The end of the movable horizontal plate 517 away from the third spring 516 is provided with an inclined surface 522 that cooperates with the conical seat 519. A second channel 515 is opened in the movable horizontal plate 517. The second channel 515 communicates with the hollow shaft 34. The movable horizontal plate 517 is provided with second through holes 521 at equal intervals at the bottom of the second channel 515. The bottom of the hollow shaft 34 is provided with first through holes 518 at equal intervals that cooperate with the second through holes 521. When the lower end of the side wall of the tapered seat 519 contacts the top of the inclined surface 522 of the movable horizontal plate 517 at the corresponding position, the second through hole 521 and the first through hole 518 are misaligned; when the upper end of the side wall of the tapered seat 519 contacts the top of the inclined surface 522 of the movable horizontal plate 517 at the corresponding position, the second through hole 521 and the first through hole 518 are coincident. When oxygen is not added or the fermentation liquid is not circulated, the second cylinder 52 drives the pressure plate 58 to move upward, the second spring 59 drives the square rod 510 to move upward, the square rod 510 drives the conical seat 519 to move upward, and at the same time, the third spring 516 drives the movable horizontal plate 517 to move towards the stirring rod 35 until the lower end of the side wall of the conical seat 519 contacts the top of the inclined surface 522 opened by the movable horizontal plate 517 at the corresponding position. The second through hole 521 is misaligned with the first through hole 518 to block the first through hole 518, preventing liquid and material from entering the second through hole 521, the second channel 515, and the hollow shaft 34. When oxygen needs to be added, the second cylinder 52 of the adjustable feed assembly 5 moves the pressure plate 58 downward, the pressure plate 58 moves the square rod 510 downward, the square rod 510 moves the conical seat 519 downward, and the conical seat 519 moves downward. The conical seat 519, through the inclined surface 522, moves the movable horizontal plate 517 along the transverse slide groove 520 away from the stirring rod 35 until the upper end of the side wall of the conical seat 519 contacts the top of the inclined surface 522 of the movable horizontal plate 517 at the corresponding position. The second through hole 521 coincides with the first through hole 518, and the outside gas enters through the air intake. The tube 55 enters the gas guide slip ring 56, and then enters the hollow shaft 34. The first piston 511 and the second piston 512 cooperate to guide the gas into the first channel 513, and then spray it out from the first channel 513 to below the third piston 514. Then it enters the second channel 515 except for the uppermost layer, and then sprays it out from the first through hole 518 opened by the stirring rod 35. At the same time, the stirring rod 35 drives the first through hole 518 to rotate and connect, so as to realize the rotation of the first through hole 518 to inject oxygen. The rotation is used to inject oxygen evenly, which helps to add oxygen evenly and ensures the uniformity of aerobic fermentation. The fermentation broth needs to circulate. The second cylinder 52 of the adjustable feeding assembly 5 drives the pressure plate 58 to move downwards. The pressure plate 58 drives the square rod 510 to move downwards. The square rod 510 drives the conical seat 519 to move downwards. The conical seat 519 moves downwards, and through the inclined surface 522, it drives the movable horizontal plate 517 to move along the horizontal slide groove 520 away from the stirring rod 35 until the upper end of the side wall of the conical seat 519 contacts the top of the inclined surface 522 of the movable horizontal plate 517 at the corresponding position. The second through hole 521 coincides with the first through hole 518. External gas enters the gas guide ring 56 through the air inlet pipe 55, and then enters the hollow shaft 34. The first piston 511 and the second piston 512 cooperate to guide the gas into the first channel 513, and then spray it out from the first channel 513 to below the third piston 514, and then enters the... In addition to the second channel 515 at the top, the fermentation liquid is sprayed out from the first through hole 518 opened by the stirring rod 35. The liquid pump 53 draws the fermentation liquid in the straight pipe 45 through the liquid extraction pipe 54 into the liquid guiding slip ring 57. The fermentation liquid enters the space between the second piston 512 and the third piston 514 through the liquid guiding slip ring 57, and then enters the second channel 515 at the top. It is sprayed out from the first through hole 518 at the top. The stirring rod 35 drives the first through hole 518 at the top to spray the liquid, so that the fermentation liquid is evenly added to the top of the fermentation material. Then, it flows downward by its own gravity, so that all the materials can come into contact with the fermentation liquid, realize the circulation of the fermentation liquid, and avoid the fermentation liquid being completely retained at the bottom of the fermentation tank 1. This helps the materials in the fermentation tank 1 to ferment evenly and avoids the phenomenon that the materials at the bottom ferment better than the materials at the top.

[0048] Sampling assembly 2 includes a horizontal cylinder 21, a horizontal plate 22, a first spring 23, a locking rod 24, an arc-shaped limiting block 25, a sampling rod 28, a connecting rod 210, a crank handle 211, and a discharge pipe 213. The horizontal cylinder 21 is fixedly connected to the fermentation tank 1. The inner wall of the horizontal cylinder 21 is slidably connected to the outer wall of the sampling rod 28. A sampling groove 27 is provided at the middle of the top of the sampling rod 28. The end of the sampling rod 28 away from the fermentation tank 1 is fixedly connected to the connecting rod 210. The end of the connecting rod 210 furthest from the fermenter 1 is fixedly connected to the crank handle 211. The bottom of the middle section of the horizontal cylinder 21 is fixedly connected to the discharge pipe 213. The horizontal cylinder 21 has a horizontal hole 26. The outer wall of the connecting rod 210 is slidably connected to the horizontal hole 26. The top of the horizontal hole 26 is fixedly connected to the arc-shaped limiting block 25. The connecting rod 210 has a horizontal movable groove 212 and a C-shaped groove 29. The horizontal movable groove 212 is between the C-shaped groove 29 and the sampling rod 28. Sampling slot 27 is connected to horizontal movable slot 212, and horizontal movable slot 212 or C-shaped slot 29 is slidably connected to arc-shaped limiting block 25. When horizontal movable slot 212 is slidably connected to arc-shaped limiting block 25, the opening of sampling slot 27 faces upward. When the end of C-shaped slot 29 away from horizontal movable slot 212 is slidably connected to arc-shaped limiting block 25, the opening of sampling slot 27 faces downward, and sampling slot 27 is located directly above discharge pipe 213. Horizontal plate 22 is fixedly installed. The first spring 23 is fixedly installed on the top of the horizontal plate 22 and the top of the first spring 23 is fixedly connected to the locking rod 24. The bottom of the locking rod 24 is in contact with the sliding hole opened in the horizontal plate 22 and is slidably connected. The connecting rod 210 has a locking hole 214 for inserting into the locking rod 24. When the end of the C-shaped groove 29 away from the horizontal movable groove 212 is in contact with the arc-shaped limiting block 25 and is slidably connected, the locking hole 214 is located directly below the locking rod 24. When the lock hole 214 is inserted into the lock rod 24, the end of the sampling rod 28 away from the crank handle 211 is flush with the inner wall of the fermentation tank 1. When sampling is required, pull the locking rod 24 upwards to separate it from the locking hole 214, turn the crank handle 211, and the crank handle 211 drives the connecting rod 210 to rotate. The connecting rod 210 drives the C-shaped groove 29 to rotate along the arc-shaped limiting block 25. When the connecting rod 210 drives the horizontal movable groove 212 to contact the arc-shaped limiting block 25, the sampling groove 27 will face upwards. Push the crank handle 211 to move towards the fermentation tank 1. The crank handle 211 drives the connecting rod 210 to move towards the fermentation tank 1. The connecting rod 210 drives the horizontal movable groove 212 to move along the arc-shaped limiting block 25. The connecting rod 210 drives the sampling rod 28 to move into the fermentation tank 1. The sampling rod 28 drives the sampling groove 27 to move into the fermentation tank 1. The material in the fermentation tank 1 enters the sampling groove 27. Then pull the crank handle 211 to move away from the fermentation tank 1. The crank handle 211 drives the connecting rod 210 to move away from the fermentation tank 1. The connecting rod 210 moves the horizontal movable groove 212 along the arc-shaped limiting block 25, and the connecting rod 210 moves the sampling rod 28 away from the fermentation tank 1. The sampling rod 28 moves the sampling groove 27 away from the fermentation tank 1. Then, the crank handle 211 is turned, and the crank handle 211 moves the C-shaped groove 29 along the arc-shaped limiting block 25 until the end of the C-shaped groove 29 away from the horizontal movable groove 212 is in sliding contact with the arc-shaped limiting block 25. At this time, the opening of the sampling groove 27 faces downward, and the material in the sampling groove 27 falls into the discharge pipe 213 by gravity and is discharged, realizing the sampling of the material in the fermentation tank 1. Then, the locking rod 24 is released, and the first spring 23 drives the locking rod 24 to be inserted into the locking hole 214, locking the connecting rod 210 and locking the sampling rod 28 to prevent the sampling rod 28 from moving and to prevent the sampling rod 28 from entering the fermentation tank 1.

[0049] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A bio-fermented compound feed, characterized in that, The feed, by weight, comprises the following components: 48-50 parts corn, 12.5-13.5 parts soybean meal, 7.8-8.2 parts extruded soybeans, 9.7-10.3 parts rice flour, 2.8-3.2 parts soybean protein concentrate, 3.95-4.15 parts tea seed meal, 0.96-1.04 parts honeysuckle stem and leaf powder, 4.85-5.25 parts mung bean powder, 0.96-1.05 parts choline chloride, 0.23-0.26 parts sodium chloride, 0.77-0.84 parts dicalcium phosphate, and stone... 0.45-0.55 parts powder, 1.95-2.04 parts trace element premix, 0.03-0.05 parts compound vitamins, 0.62-0.68 parts lysine phosphate, 0.33-0.36 parts methionine, 0.24-0.26 parts threonine, 0.09-0.11 parts tryptophan, 0.28-0.32 parts compound enzyme, 2.3-3.8 parts Bacillus coagulans, 2-5 parts yeast fermentation liquid, 3.5-5.5 parts Enterococcus faecalis and 4.5-5.5 parts Bacillus subtilis.

2. The preparation process according to claim 1, characterized in that, The complex enzyme includes lipase and alkaline protease, with lipase added at 3 u / g and alkaline protease added at 0.6 u / g.

3. The preparation process according to claim 1, characterized in that, The yeast fermentation broth is a mixed broth of Candida utilis and Saccharomyces cerevisiae in a mass ratio of 1.2:

1.

4. A preparation process for a bio-fermented compound feed according to claim 1, characterized in that, Includes the following steps: Step 1: Grind tea seed meal, honeysuckle stems and leaves, and mung bean powder, mix with water, and then sterilize. Step 2: Add pure cultures of Bacillus coagulans, yeast fermentation liquid, Enterococcus faecalis, Bacillus subtilis, and compound enzymes to the sterilized material, then add compound vitamins and trace elements and mix. Step 3: Adjust the fermentation device to 28-30℃, then add the mixture into the fermentation device and incubate aerobically for 36-48 hours; Step 4: After the aerobic stage is completed, break up and mix the fermented feed, put it into a one-way venting sealed bag, and place it at room temperature for anaerobic culture for 4-8 days.

5. A fermentation apparatus for the preparation process of the bio-fermented compound feed according to claim 4, characterized in that, It includes a fermenter (1), and the top of the fermenter (1) is connected to a stirring assembly (3) for improving the mixing degree. The top of the fermenter (1) is also connected to an adjustable feed assembly (5) for uniform oxygen injection and fermentation liquid circulation. The adjustable feed assembly (5) is connected to the stirring assembly (3). The bottom of the fermentation tank (1) is fixedly connected to an adjustable discharge assembly (4) for filtering the fermentation liquid during fermentation and guiding the material during discharge. The adjustable discharge assembly (4) is connected to the adjustable feed assembly (5). The air outlet and liquid outlet of the adjustable feed assembly (5) are both located at the stirring end of the stirring assembly (3). A sampling component (2) for sampling is connected to the lower end of the side wall of the fermenter (1).

6. The fermentation equipment according to claim 5, characterized in that, The stirring assembly (3) includes a motor (31), a first bevel gear (32), a second bevel gear (33), a hollow shaft (34), and stirring rods (35). The motor (31) is fixedly installed on the top of the fermenter (1). The first bevel gear (32) is fixedly installed on the drive end of the motor (31). The first bevel gear (32) meshes with the second bevel gear (33). The second bevel gear (33) is fixedly installed on the upper end of the hollow shaft (34). The top of the fermenter (1) is rotatably connected to the hollow shaft (34) through a bearing. Multiple sets of stirring rods (35) are fixedly installed on the part of the hollow shaft (34) located inside the fermenter (1). The hollow shaft (34) and the stirring rods (35) are both connected to the adjustable feed assembly (5). The gas outlet and liquid outlet of the adjustable feed assembly (5) are both located on the stirring rods (35).

7. The fermentation equipment according to claim 6, characterized in that, The adjustable discharge assembly (4) includes a connecting plate (41), a sliding plate (42), a protective cover (43), a first cylinder (44), a straight pipe (45), a gate (46), and a filter plate (47). The straight pipe (45) is fixedly installed at the bottom of the fermenter (1). Two sets of protective covers (43) are fixedly installed on the outer wall of the straight pipe (45). The first cylinder (44) is fixedly connected to the protective cover (43). The connecting plate (41) is fixedly installed at the drive end of the first cylinder (44). Two sets of sliding plates (42) are fixedly installed on the connecting plate (41). On the upper part, two sets of sliding plates (42) are fixedly connected to the gate (46) and the filter plate (47) respectively. The gate (46) and the filter plate (47) are slidably connected to the inner wall of the protective cover (43) respectively. The gate (46) and the filter plate (47) are slidably connected to the horizontal sliding hole opened in the straight pipe (45). The filter plate (47) is located above the gate (46). The adjustable feeding assembly (5) is fixedly connected to the straight pipe (45). The connection between the adjustable feeding assembly (5) and the straight pipe (45) is located between the gate (46) and the filter plate (47).

8. The fermentation equipment according to claim 7, characterized in that, The adjustable feed assembly (5) includes an air intake assembly, a liquid addition assembly, a follow-up conductive assembly and an adjustment assembly. The adjustment assembly is fixedly connected to the fermenter (1). The adjustment assembly, air intake assembly and liquid addition assembly are connected to the hollow shaft (34). The follow-up conductive assembly is connected to the stirring rod (35). The follow-up conductive assembly and the adjustment assembly are connected.

9. The fermentation equipment according to claim 8, characterized in that, The liquid addition assembly includes a liquid pump (53), a liquid extraction pipe (54), and a liquid guide slip ring (57). The outer rings of the liquid pump (53) and the liquid guide slip ring (57) are fixedly connected to the top of the fermenter (1). The inner ring of the liquid guide slip ring (57) is fixedly connected to the hollow shaft (34). The liquid guide slip ring (57) communicates with the hollow shaft (34). The outer ring of the liquid guide slip ring (57) is fixedly connected to the output end of the liquid pump (53) through a pipe. The input end of the liquid pump (53) is fixedly connected to the liquid extraction pipe (54). The liquid extraction pipe (54) is fixedly connected to the straight pipe (45), and the connection between the liquid extraction pipe (54) and the straight pipe (45) is located between the gate (46) and the filter plate (47).

10. The fermentation equipment according to claim 9, characterized in that, The air intake assembly includes an air intake pipe (55) and an air guide slip ring (56). The outer ring of the air guide slip ring (56) is fixedly connected to the outer ring of the liquid guide slip ring (57). The inner ring of the air guide slip ring (56) is fixedly connected to the hollow shaft (34). The air guide slip ring (56) is connected to the hollow shaft (34).

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