Feed additive composition and preparation method thereof

Abstract

The invention relates to the technical field of feed additives, in particular to a feed additive composition and a preparation method thereof, and the feed additive composition comprises the following components in parts by mass: 15-20 parts of a plant extract, 0.1-5 parts of a compound microbial agent, 2-8 parts of a rosemary extract, 1-10 parts of phellinus igniarius polysaccharide, 0.1-3 parts of citric acid, 0.1-2 parts of amino acid, 0.1-2 parts of tween-80, 0.1-1 part of trace elements and 5-30 parts of lecithin. By adopting the components, the phellinus igniarius polysaccharide and the fructus lycii extract cooperate to reduce the morbidity of piglet diarrhea, the oxidative degradation rate of the plant extract is reduced through lipidosome coating, the survival rate of the compound microbial agent in the intestinal tract is improved, and enzyme secreted by the compound microbial agent cooperates with the plant extract in the lipidosome to improve the feed conversion efficiency.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of feed additives, in particular to a feed additive composition and a preparation method thereof. BACKGROUND

[0002] The current livestock breeding industry is facing two major challenges. One is the problem of drug resistance and food safety caused by the abuse of antibiotics. Traditional feed relies on antibiotics to prevent intestinal diseases and promote growth, but long-term use will cause drug residues in the animal body. The second is the problem of low utilization rate and poor stability of feed nutrients. Plant-derived active ingredients (such as flavonoids and polysaccharides) are easily oxidized and degraded by temperature and oxygen during storage and processing. At the same time, the existing plant-derived feed additives have obvious limitations. Single plant extracts have single function (such as focusing only on antioxidant or digestion promotion), and lack of multi-function synergy. Microbial agents are mostly directly added without protection measures, and are easily destroyed by gastric acid and bile salts after entering the animal intestine, making it difficult to colonize and play a role. SUMMARY

[0003] The purpose of the present application is to provide a feed additive composition and a preparation method thereof. Liposome coating reduces the oxidation and degradation rate of plant extracts, and the survival rate of complex microbial agents in the intestine is improved.

[0004] To achieve the above-mentioned purpose, the present application provides a feed additive composition, which comprises plant extracts 15-20 parts, complex microbial agents 0.1-5 parts, rosemary extracts 2-8 parts, Sanghuang polysaccharides 1-10 parts, citric acid 0.1-3 parts, amino acids 0.1-2 parts, Tween-80 0.1-2 parts, trace elements 0.1-1 parts and lecithin 5-30 parts by mass fraction.

[0005] Preferably, the plant extracts include one or more of Toona sinensis extract, Eucommia ulmoides leaf extract, Portulaca oleracea extract, Pericarpium Citri Reticulatae extract, Atractylodes macrocephala Koidz extract, glycerol and Lycium barbarum extract.

[0006] Preferably, the complex microbial agents include one or more of acidophilic lactic acid bacteria, Lactobacillus plantarum, Bifidobacterium, Bacillus coagulans, Clostridium butyricum and Bacillus subtilis.

[0007] Preferably, the trace elements include one or more of zinc, calcium, iron, manganese, copper and selenium.

[0008] Preferably, the amino acids include one or more of hydroxyproline, glutamic acid, glycine and taurine.

[0009] A preparation method of a feed additive composition, which prepares the above-mentioned feed additive composition, includes the following steps, S1, plant extract, rosemary extract, phellinus baumii polysaccharide, amino acid, citric acid and trace element mixture are taken by mass fraction to obtain a mixture; S2, the mixture in S1 is put into an airflow type ultrafine grinder to be crushed, and sieving is performed to obtain an ultrafine powder mixture; S3, 10-30% of Tween-80 and physiological saline are sequentially added to the ultrafine powder mixture obtained in S2 to be uniformly mixed to obtain a core mixture; S4, lecithin is dissolved in the remaining Tween-80, and physiological saline is added to be uniformly stirred to obtain a shell solution; S5, the core mixture in S3 is mixed with the shell solution in S4, and high-pressure homogenization is performed, sample detection is performed, and the lipid suspension is obtained after quality inspection; S6, the compound microbial agent is dissolved in physiological saline to obtain a compound microbial agent solution, the compound microbial agent solution is slowly added to the lipid suspension obtained in S5 to be uniformly stirred to obtain a compound microbial-liposome suspension; S7, a freeze-drying protective agent is added to the compound microbial-liposome suspension, the pH is adjusted, vacuum freeze-drying is performed, and sieving is performed after crushing to obtain a feed additive composition.

[0010] Preferably, in S2, nitrogen is introduced during ultrafine crushing, and the crushing temperature is maintained at 20-25 DEG C.

[0011] Preferably, in S5, high-pressure homogenization is performed at 10-30 MPa for 2-3 times and at 50-80 MPa for 5-6 times, and the homogenization temperature is 25-35 DEG C.

[0012] Preferably, in S6, the temperature of slow stirring is 10-25 DEG C, and the speed of slow stirring is 60-80 r / min.

[0013] Preferably, in S7, the pH is 5-7, the freeze-drying temperature is -30 to -20 DEG C, and the freeze-drying time is 10-24 h.

[0014] Therefore, the feed additive composition and the preparation method thereof have the following beneficial effects: 1, the feed additive composition provided by the present application does not add antibiotics, and relies on the natural antibacterial property of plant extract (Chinese toon, eucommia etc.) and the intestinal regulation function of microbial agent to prevent diseases; 2, the enzyme secreted by the compound microbial agent in the feed additive composition provided by the present application cooperates with the plant extract in the liposome to improve the feed conversion efficiency; 3. The feed additive composition provided by this invention has the synergistic effect of Phellinus linteus polysaccharide and Lycium barbarum extract, which reduces the incidence of diarrhea in piglets. The liposome encapsulation reduces the oxidative degradation rate of plant extracts, improves the survival rate of compound microbial agents in the intestine, and the product reconstitutes quickly after freeze-drying.

[0015] The technical solution of the present invention will be further described in detail below through embodiments. Detailed Implementation

[0016] The present invention will be further described below with reference to embodiments. Unless otherwise defined, the technical or scientific terms used in this invention should have the ordinary meaning understood by one of ordinary skill in the art. The features mentioned above or in the specific examples mentioned in this invention can be combined arbitrarily, and these specific embodiments are only used to illustrate the invention and are not intended to limit the scope of the invention.

[0017] This invention provides a feed additive composition comprising, by weight, 15-20 parts of plant extract, 0.1-5 parts of compound microbial agent, 2-8 parts of rosemary extract, 1-10 parts of Phellinus linteus polysaccharide, 0.1-3 parts of citric acid, 0.1-2 parts of amino acids, 0.1-2 parts of Tween-80, 0.1-1 parts of trace elements, and 5-30 parts of lecithin.

[0018] In some embodiments of the present invention, the plant extracts include one or more of the following: Toona sinensis extract, Eucommia ulmoides leaf extract, Portulaca oleracea extract, Citrus reticulata peel extract, Atractylodes macrocephala extract, glycoterpenes, and Lycium barbarum extract. Toona sinensis extract contains toona sinensis, which has antibacterial and digestive-promoting properties; Eucommia ulmoides leaf extract contains chlorogenic acid, which has antioxidant and cholesterol-lowering properties; Portulaca oleracea extract contains Portulaca oleracea polysaccharide, which has anti-inflammatory and antidiarrheal properties; Citrus reticulata peel extract has qi-regulating and spleen-strengthening properties; Atractylodes macrocephala extract contains atractylodes lactone, which regulates intestinal peristalsis; and Lycium barbarum extract contains betaine, which promotes fat metabolism. Glycoterpenes improve intestinal health and digestion and absorption, and significantly enhance the neuroendocrine and immune functions and disease resistance and stress resistance of animals. The combined use of these plant extracts addresses the problem of diarrhea and weak immunity in young animals (such as piglets and chicks) by reducing morbidity through multi-target action.

[0019] In some embodiments of the present invention, the compound microbial agent includes one or more of the following: Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium, Bacillus coagulans, Clostridium butyricum, and Bacillus subtilis. Bacillus subtilis and Bacillus coagulans can form spores and are resistant to high temperatures during feed processing; Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium are native intestinal bacteria, easily colonize, and produce lactic acid to regulate intestinal pH. Clostridium butyricum promotes the proliferation and development of beneficial intestinal flora (Bifidobacterium and Lactobacillus), inhibits the growth and reproduction of harmful and putrefactive bacteria in the intestine, corrects intestinal flora imbalance, reduces the occurrence of enterotoxins, and its main metabolite, butyric acid, is a major nutrient for the regeneration and repair of intestinal epithelial cells.

[0020] In some embodiments of the present application, the trace elements include one or more of zinc, calcium, iron, manganese, copper and selenium. Zinc promotes enzyme activity, iron synthesizes hemoglobin, manganese promotes bone development, copper promotes melanin synthesis, and selenium is an antioxidant enzyme coenzyme essential for animals.

[0021] In some embodiments of the present application, the amino acids include one or more of hydroxyproline, glutamic acid, glycine and taurine. Hydroxyproline is a precursor of collagen synthesis, which enhances the integrity of the intestinal mucosa. Glutamic acid improves palatability and promotes feed intake, while also serving as a neurotransmitter to regulate intestinal peristalsis. Glycine is an antioxidant that synergistically scavenges free radicals with rosemary extract. Taurine regulates lipid metabolism and improves meat tenderness.

[0022] A method for preparing a feed additive composition, the method for preparing the feed additive composition described above, comprising the steps of, S1. Plant extract, rosemary extract, Sanghuang polysaccharide, amino acid, citric acid, trace elements are weighed by mass fraction to obtain a mixture; S2. The mixture in S1 is put into an airflow type ultrafine pulverizer for pulverization, and sieving is performed to obtain an ultrafine powder mixture; S3. 10-30% of Tween-80 and physiological saline are sequentially added to the ultrafine powder mixture obtained in S2 and mixed uniformly to obtain a core mixture; S4. Lecithin is dissolved in the remaining Tween-80, and physiological saline is added and stirred uniformly to obtain a shell solution; S5. The core mixture in S3 is mixed with the shell solution in S4, and high-pressure homogenization is performed, and sampling and quality inspection are performed to obtain a liposome suspension after quality inspection is passed; S6. The compound microbial agent is dissolved in physiological saline to obtain a compound microbial agent solution, and the compound microbial agent solution is slowly added to the liposome suspension obtained in S5, and is stirred uniformly to obtain a compound microbial-liposome suspension; S7. A freeze-drying protective agent is added to the compound microbial-liposome suspension, the pH is adjusted, vacuum freeze-drying is performed, and sieving is performed after pulverization to obtain a feed additive composition.

[0023] In some embodiments of the present application, in S2, nitrogen is introduced during the ultrafine grinding, and the grinding temperature is maintained at 20-25 DEG C. The nitrogen can isolate oxygen, and the low-temperature nitrogen (20-25 DEG C) can carry away the heat generated during the grinding, thereby preventing the polyphenols in the rosemary extract and the flavones in the plant extract from being oxidized and browned due to high temperature and oxygen, preventing the amino acids from being denatured due to high temperature, and ensuring the activity retention rate of the components after the grinding. The particle size of the ultrafine powder mixture is 1-10 microns, which facilitates the formation of a mild mixing system with the microbial agent in the subsequent step, avoids the inactivation of the microbial agent, and enables the fine particles to be uniformly dispersed in the freeze-drying protective agent during the subsequent freeze-drying, thereby further protecting the activity of the microbial agent.

[0024] In some embodiments of the present application, in S5, the high-pressure homogenization is performed at 10-30 MPa for 2-3 times and at 50-80 MPa for 5-6 times, and the homogenization temperature is 25-35 DEG C. The low-pressure preliminary emulsification forms a stable emulsion of the core and the shell. The high-pressure fine lipid particle size ensures the encapsulation efficiency and the dispersibility. The homogenization temperature of 25-35 DEG C avoids the excessive oxidation or coagulation of lecithin, and prevents the degradation of the chamonix polysaccharide in the core due to high temperature.

[0025] In some embodiments of the present application, the average particle size of the liposome obtained in S5 is controlled to be 80-150 nm (PDI≤0.3), which can pass through the intercellular space of the intestinal epithelial cells and avoid the decrease in the absorption efficiency caused by the aggregation.

[0026] In some embodiments of the present application, in S6, the temperature of the slow stirring is 10-25 DEG C, and the speed of the slow stirring is 60-80 r / min. The slow stirring ensures the suitable temperature of the microorganism and avoids the damage to the cell wall of the microorganism during the stirring.

[0027] In some embodiments of the present application, in S7, the pH is 5-7, the freeze-drying temperature is -30 to -20 DEG C, and the freeze-drying time is 10-24 h. The slow freezing of the material avoids the formation of large ice crystals, and the pH of 5-7 is suitable for the stable interval of the microorganism and the liposome.

[0028] Example 1 S1, the plant extract 18 parts (the mixture of the citrus peel extract, the white atractylodes rhizome extract and the medlar extract at a mass ratio of 1:1:2), the rosemary extract 5 parts, the chamonix polysaccharide 8 parts, the amino acid 1 part (the mixture of glycine and taurine at a mass ratio of 1:1), the citric acid 1 part and the trace elements 0.5 parts (the mixture of calcium, iron and manganese at a mass ratio of 1:1:1) are weighed according to the mass fraction, and then mixed to obtain a mixture.

[0029] S2, the mixture in S1 is put into an airflow type ultrafine grinder, nitrogen is introduced during the ultrafine grinding, the grinding temperature is maintained at 25 DEG C, and an ultrafine powder mixture is obtained by passing through a 200-mesh sieve.

[0030] S3, add 0.2 parts of Tween-80 and 150 parts of physiological saline to the ultra-fine powder mixture obtained in S2 in turn, mix uniformly, filter with a 0.22 μm microporous filter, and obtain a core mixture.

[0031] S4, dissolve 30 parts of lecithin in the remaining 0.8 parts of Tween-80, add 220 parts of physiological saline, stir uniformly at 50°C, and obtain a shell solution.

[0032] S5, mix the core mixture in S3 with the shell solution in S4 at a volume ratio of 1:2, and then high-pressure homogenize, first homogenize 3 times at 20 MPa, and then homogenize 6 times at 60 MPa, with a homogenization temperature of 25°C. Sampling detection shows that the average particle size is 100 nm, the encapsulation rate is greater than 85%, and the quality inspection is qualified, thereby obtaining a liposome suspension.

[0033] S6, dissolve 3 parts of a composite microbial agent (mass-mixed acidophilic lactic acid bacteria, lactobacillus plantarum, bifidobacterium, bacillus coagulans, clostridium butyricum, and bacillus subtilis) in physiological saline to obtain a composite microbial agent solution, and activate for 30 min at 37°C. Slowly add 10 parts of the composite microbial agent solution to the liposome suspension obtained in S5 at 25°C, and stir uniformly at 70 r / min to obtain a composite microbial-liposome suspension.

[0034] S7, add a freeze-drying protective agent (sucrose and mannitol at a mass ratio of 3:1) in an amount of 10% of the mass of the composite microbial-liposome suspension to the composite microbial-liposome suspension, and adjust the pH to 5.5. Vacuum freeze-dry, with a freeze-drying temperature of -25°C, a freeze-drying time of 18 h, and a constant-temperature desorption drying time of 8 h at 30°C. After crushing and passing through an 80-mesh sieve, a feed additive composition is obtained, which is immediately vacuum-packed and stored.

[0035] Example 2 S1, mix 15 parts of a solid plant extract (mass-mixed tospinasterone, eucommia leaf extract, and glycerol at a mass ratio of 1:0.5:1), 2 parts of rosemary extract, 2 parts of sanghuang polysaccharide, 0.1 parts of amino acids (mass-mixed hydroxyproline and glutamic acid at a ratio of 1:1), 0.2 parts of citric acid, and 0.1 parts of trace elements (mass-mixed calcium, iron, and manganese at a mass ratio of 1:1:1) to obtain a mixture.

[0036] S2, place the mixture in S1 into an airflow type ultrafine grinder, and pulverize while introducing nitrogen, with a pulverization temperature of 25°C, and pass through a 200-mesh sieve to obtain an ultra-fine powder mixture.

[0037] S3, add 0.2 parts of Tween-80 and 150 parts of physiological saline to the ultra-fine powder mixture obtained in S2 in turn, mix uniformly, filter with a 0.22 μm microporous filter, and obtain a core mixture.

[0038] S4, 20 parts of lecithin were dissolved in the remaining 0.8 parts of Tween-80, 160 parts of normal saline were added, and the mixture was stirred uniformly at 50°C to obtain a shell solution.

[0039] S5, the inner core mixture in S3 was mixed with the shell solution in S4 at a volume ratio of 1:2, and then high-pressure homogenization was performed, first at 20 MPa for 3 times and then at 60 MPa for 6 times, with a homogenization temperature of 25°C. Sampling detection showed that the average particle size was 100 nm and the encapsulation rate was greater than 85%. After quality inspection, a liposome suspension was obtained.

[0040] S6, 3 parts of a complex microbial agent (mass mixture of acidophilic lactic acid bacteria, lactobacillus plantarum, bifidobacterium, bacillus coagulans, clostridium butyricum, and bacillus subtilis) were dissolved in 10 parts of normal saline to obtain a complex microbial agent solution, which was activated at 37°C for 30 min. The complex microbial agent solution was slowly added to the liposome suspension obtained in S5 at 25°C, and the mixture was stirred uniformly at 70 r / min to obtain a complex microbial-liposome suspension.

[0041] S7, 10% of a freeze-drying protectant (sucrose and mannitol in a mass ratio of 3:1) based on the mass of the complex microbial-liposome suspension was added to the complex microbial-liposome suspension, and the pH was adjusted to 5.5. Vacuum freeze-drying was performed at a freeze-drying temperature of -25°C for 18 h, and then the temperature was increased to 30°C for constant-temperature desiccation for 8 h. After being crushed and passed through an 80-mesh sieve, a feed additive composition was obtained, which was immediately vacuum-packed and stored.

[0042] Example 3 S1, 18 parts of a solid plant extract (a mixture of Chinese toon extract and medlar extract in a mass ratio of 1:1), 6 parts of rosemary extract, 5 parts of sanghuang polysaccharide, 0.2 parts of amino acid (a mixture of glycine and taurine in a mass ratio of 1:1), 0.1 parts of citric acid, and 0.8 parts of trace elements (a mixture of zinc, calcium, and iron in a mass ratio of 1:1:1) were weighed according to the mass fraction to obtain a mixture.

[0043] S2, the mixture in S1 was crushed in an airflow type ultrafine grinder, nitrogen was introduced during ultrafine grinding, the crushing temperature was maintained at 25°C, and an ultrafine powder mixture was obtained by passing through a 200-mesh sieve.

[0044] S3, 0.2 parts of Tween-80 and 150 parts of normal saline were sequentially added to the ultrafine powder mixture obtained in S2, the mixture was uniformly mixed, and then filtered through a 0.22 μm microporous filter to obtain an inner core mixture.

[0045] S4, 30 parts of lecithin were dissolved in the remaining 0.8 parts of Tween-80, 220 parts of normal saline were added, and the mixture was stirred uniformly at 50°C to obtain a shell solution.

[0046] S5, the core mixture in S3 is mixed with the shell solution in S4 at a volume ratio of 1:2, and then high-pressure homogenization is performed, first at 20 MPa for 3 times and then at 60 MPa for 6 times, with a homogenization temperature of 25°C. Sampling detection shows that the average particle size is 100 nm and the encapsulation rate is greater than 85%, and after quality inspection, a liposome suspension is obtained.

[0047] S6, 3 parts of the complex microbial agent (mass mixture of acidophilic lactic acid bacteria, lactobacillus plantarum, bifidobacterium, bacillus coagulans, clostridium butyricum, and bacillus subtilis) are dissolved in 10 parts of physiological saline to obtain a complex microbial agent solution, which is activated at 37°C for 30 min. The complex microbial agent solution is slowly added to the liposome suspension obtained in S5 at 25°C, and then slowly stirred at 70 r / min to obtain a complex microbial-liposome suspension.

[0048] S7, 10% of the complex microbial-liposome suspension by mass of freeze-drying protectant (sucrose and mannitol at a mass ratio of 3:1) is added to the complex microbial-liposome suspension, and the pH is adjusted to 5.5. Vacuum freeze-drying is performed at a freeze-drying temperature of -25°C for 18 h, and then the temperature is increased to 30°C, and constant-temperature desiccation is performed for 8 h. After crushing and passing through an 80-mesh sieve, a feed additive composition is obtained, which is immediately vacuum-packed and stored.

[0049] Example 4 S1, solid plant extracts 16 parts (a mixture of pericarpium citri reticulatae extract and atractylodes extract at a mass ratio of 1:1), rosemary extract 3 parts, morus albus polysaccharide 4 parts, amino acid 1 part (a mixture of hydroxyproline and taurine at a mass ratio of 1:1), citric acid 0.8 parts, and trace elements 0.4 parts (a mixture of manganese, copper, and selenium at a mass ratio of 1:1:1) are mixed to obtain a mixture.

[0050] S2, the mixture in S1 is crushed in an airflow type ultrafine grinder, nitrogen is introduced during ultrafine crushing, the crushing temperature is maintained at 25°C, and an ultrafine powder mixture is obtained by passing through a 200-mesh sieve.

[0051] S3, 0.2 parts of Tween-80 and 150 parts of physiological saline are sequentially added to the ultrafine powder mixture obtained in S2, mixed uniformly, filtered with a 0.22 μm microporous filter, and a core mixture is obtained.

[0052] S4, lecithin 30 parts is dissolved in the remaining 0.8 parts of Tween-80, 220 parts of physiological saline is added, and stirring is performed uniformly at 50°C to obtain a shell solution.

[0053] S5, the core mixture in S3 is mixed with the shell solution in S4 at a volume ratio of 1:2, and then high-pressure homogenization is performed, first at 20 MPa for 3 times and then at 60 MPa for 6 times, with a homogenization temperature of 25°C. Sampling detection shows that the average particle size is 100 nm and the encapsulation rate is greater than 85%, and after quality inspection, a liposome suspension is obtained.

[0054] S6, 3 parts of the composite microbial agent (mass mixture of acidophilic lactic acid bacteria, lactobacillus plantarum, bifidobacterium, bacillus coagulans, clostridium butyricum and bacillus subtilis) are dissolved in 10 parts of physiological saline to obtain a composite microbial agent solution, which is activated at 37°C for 30 min. The composite microbial agent solution is slowly added to the liposome suspension obtained in S5 at 25°C, and then uniformly stirred at 70 r / min to obtain a composite microbial-liposome suspension.

[0055] S7, 10% of the freeze-drying protective agent (mass ratio of sucrose to mannitol is 3:1) of the composite microbial-liposome suspension is added to the composite microbial-liposome suspension, and the pH is adjusted to 5.5. Vacuum freeze-drying is performed at a freeze-drying temperature of -25°C for 18 h, and then the temperature is increased to 30°C, and constant temperature desorption drying is performed for 8 h. After crushing and passing through an 80-mesh sieve, a feed additive composition is obtained, which is immediately vacuum packaged and stored.

[0056] Example 5 S1, solid plant extracts 19 parts (chinese toon extract and dried tangerine peel extract mixed at a mass ratio of 1:1), rosemary extract 7 parts, morus albus polysaccharide 8 parts, amino acid 1.2 parts (glutamic acid and glycine mixed at a mass ratio of 1:1), citric acid 1 part, and trace elements 0.7 parts (calcium, iron and selenium mixed at a mass ratio of 1:1:1) are weighed according to the mass fraction to obtain a mixture.

[0057] S2, the mixture in S1 is put into an airflow type ultrafine grinder, and nitrogen is introduced during ultrafine grinding, with a grinding temperature of 25°C. After passing through a 200-mesh sieve, an ultrafine powder mixture is obtained.

[0058] S3, 0.2 parts of Tween-80 and 150 parts of physiological saline are added to the ultrafine powder mixture obtained in S2 in sequence, mixed uniformly, and filtered with a 0.22 μm microporous filter to obtain a core mixture.

[0059] S4, lecithin 30 parts is dissolved in the remaining 0.8 parts of Tween-80, 220 parts of physiological saline is added, and stirring is performed uniformly at 50°C to obtain a shell solution.

[0060] S5, the core mixture in S3 is mixed with the shell solution in S4 at a volume ratio of 1:2, and then high-pressure homogenization is performed, first at 20 MPa for 3 times and then at 60 MPa for 6 times, with a homogenization temperature of 25°C. Sampling detection shows that the average particle size is 100 nm and the encapsulation rate is greater than 85%, and after quality inspection, a liposome suspension is obtained.

[0061] S6, 3 parts of the composite microbial agent (mass-mixed acidophilic lactic acid bacteria, lactobacillus plantarum, bifidobacterium, bacillus coagulans, clostridium butyricum, and bacillus subtilis) are dissolved in 10 parts of physiological saline to obtain a composite microbial agent solution, which is activated at 37°C for 30 min. The composite microbial agent solution is slowly added to the liposome suspension obtained in S5 at 25°C, and then slowly stirred at 70 r / min to obtain a composite microbial-liposome suspension.

[0062] S7, 10% of the freeze-drying protective agent (sucrose and mannitol at a mass ratio of 3:1) based on the mass of the composite microbial-liposome suspension is added to the composite microbial-liposome suspension, and the pH is adjusted to 5.5. Vacuum freeze-drying is performed at a freeze-drying temperature of -25°C for 18 h, and then the temperature is increased to 30°C for constant-temperature desiccation for 8 h. After crushing and passing through an 80-mesh sieve, a feed additive composition is obtained, which is immediately vacuum-packed and stored.

[0063] Comparative Example 1 S1, solid plant extracts are weighed according to the mass fraction, 18 parts of Chinese toona extract, 6 parts of rosemary extract, 5 parts of phellinus igniarius polysaccharide, 0.2 parts of amino acid (glycine and taurine mixed at a mass ratio of 1:1), 0.1 parts of citric acid, and 0.8 parts of trace elements (zinc, calcium, and iron mixed at a mass ratio of 1:1:1) are mixed to obtain a mixture.

[0064] S2, the mixture in S1 is put into an airflow-type ultrafine grinder, and nitrogen is introduced during ultrafine grinding, with a grinding temperature of 25°C. After passing through a 200-mesh sieve, an ultrafine powder mixture is obtained.

[0065] S3, 3 parts of the composite microbial agent (mass-mixed acidophilic lactic acid bacteria, lactobacillus plantarum, bifidobacterium, bacillus coagulans, and bacillus subtilis) are dissolved in 100 parts of physiological saline to obtain a composite microbial agent solution, which is activated at 37°C for 30 min. The ultrafine powder mixture obtained in S2 is slowly added at 25°C, and then slowly stirred at 70 r / min to obtain a composite microbial mixture.

[0066] S4, to the composite microbial mixture, 10% of the mass of the composite microbial mixture of freeze-drying protective agent (sucrose and mannitol mass ratio 3:1) is added, and the pH is adjusted to 5.5. Vacuum freeze-drying, freeze-drying temperature is-25℃, freeze-drying time is 18h, continue to warm up to 30℃, constant temperature desorption drying for 8 hours, crush and pass through 80 mesh sieve, get the feed additive composition, immediately vacuum package and store.

[0067] Test example Select weaned piglets, growing pigs and fattening pigs with similar growth conditions and half male and half female, divide into eight groups, four groups as experimental groups add feed additive in example 3 in feed, another four groups as control group add feed additive in comparative example 1 in feed, the feed type, feed quality and feed additive addition quality of two groups are same. The daily feeding amount and frequency of each group are same, feed for 28 days, and the diarrhea rate, average daily weight gain and digestibility of the first day and the 28th day are measured respectively, and the results are shown in table 1.

[0068] Table 1, data table of experimental group and control group before and after feeding

[0069] As can be seen from table 1, the effect of feed additive composition in example 3 in feed is better than that of feed additive composition in comparative example 1, which shows that the construction of liposome and the compounding of various plant extracts have excellent effect on the absorption and conversion of feed, and reduce the incidence of weaned piglets.

[0070] Therefore, the present application adopts the above-mentioned feed additive composition and its preparation method, the oxidation degradation rate of plant extract is reduced by liposome coating, and the survival rate of composite microbial agent in the intestinal tract is improved.

[0071] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application but not to limit it, although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can still be modified or replaced by equivalents, and these modifications or equivalent replacements cannot make the modified technical solutions deviate from the spirit and scope of the technical solutions of the present application.

Claims

1. A feed additive composition, characterized in that: The ingredients, by weight, include 15-20 parts plant extract, 0.1-5 parts compound microbial agent, 2-8 parts rosemary extract, 1-10 parts Phellinus linteus polysaccharide, 0.1-3 parts citric acid, 0.1-2 parts amino acids, 0.1-2 parts Tween-80, 0.1-1 parts trace elements, and 5-30 parts lecithin.

2. The feed additive composition according to claim 1, characterized in that: Plant extracts include one or more of the following: Toona sinensis extract, Eucommia ulmoides leaf extract, Portulaca oleracea extract, Citrus reticulata peel extract, Atractylodes macrocephala extract, glycoterpenoids, and Lycium barbarum extract.

3. The feed additive composition according to claim 1, characterized in that: The compound microbial agent includes one or more of the following: Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium, Bacillus coagulans, Clostridium butyricum, and Bacillus subtilis.

4. The feed additive composition according to claim 1, characterized in that: Trace elements include one or more of zinc, calcium, iron, manganese, copper, and selenium.

5. The feed additive composition according to claim 1, characterized in that: Amino acids include one or more of hydroxyproline, glutamic acid, glycine, and taurine.

6. A method for preparing a feed additive composition, characterized in that: The preparation of a feed additive composition according to any one of claims 1-5 comprises the following steps: S1. Weigh out the plant extract, rosemary extract, Phellinus linteus polysaccharide, amino acids, citric acid, and trace elements according to the mass fractions and mix them to obtain a mixture; S2. The mixture in S1 is placed into an airflow ultra-fine pulverizer and pulverized, and then sieved to obtain an ultra-fine powder mixture. S3. Add 10-30% Tween-80 and physiological saline to the ultrafine powder mixture obtained in S2 and mix evenly to obtain the core mixture. S4. Dissolve lecithin in the remaining Tween-80, add physiological saline and stir well to obtain the shell solution; S5. After mixing the core mixture in S3 with the shell solution in S4, the mixture is homogenized under high pressure, sampled and tested, and after passing the quality inspection, a liposome suspension is obtained. S6. Dissolve the compound microbial agent in physiological saline to obtain a compound microbial agent solution. Slowly add the compound microbial agent solution to the liposome suspension obtained in S5 and stir slowly until homogeneous to obtain a compound microbial-liposome suspension. S7. Add a freeze-drying protectant to the compound microbial-liposome suspension, adjust the pH, freeze-dry under vacuum, pulverize and sieve to obtain the feed additive composition.

7. The method for preparing a feed additive composition according to claim 6, characterized in that: In S2, nitrogen gas is introduced during ultrafine grinding to maintain the grinding temperature at 20-25℃.

8. The method for preparing a feed additive composition according to claim 6, characterized in that: In S5, high-pressure homogenization involves homogenizing 2-3 times at 10-30 MPa and then homogenizing 5-6 times at 50-80 MPa, with a homogenization temperature of 25-35℃.

9. The method for preparing a feed additive composition according to claim 6, characterized in that: In S6, the temperature for slow stirring is 10-25℃, and the stirring speed is 60-80 r / min.

10. A method for preparing a feed additive composition according to claim 6, characterized in that: In S7, the pH is 5-7, the freeze-drying temperature is -30 to -20℃, and the freeze-drying time is 10-24h.

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