Application of lycopene in preparation of functional feed

Abstract

The invention discloses application of lycopene in preparation of functional feed, and relates to the technical field of livestock breeding and feed development. According to the invention, when the effective addition concentration of lycopene to silkworms is 5mg / kg / day, the organism immunity of the silkworms can be effectively improved, meanwhile, the reasonable growth of the body weight of the silkworms is promoted, the athletic ability of the silkworms is remarkably enhanced, and the hatching rate and the yield of silkworm eggs are remarkably improved; therefore, the lycopene has the important potential of being used as an auxiliary functional component for preparing the livestock and poultry functional feed, has the advantages of being low in addition amount, good in palatability and free of residues, and provides a new direction for expansion and application of the lycopene in the livestock and poultry breeding field. And coping strategies and solutions can be provided for effectively solving the problems of product effective hidden dangers caused by single function and antibiotic abuse of current livestock and poultry feeds, insufficient vitality of livestock and poultry animals, low reproductive efficiency and the like.

Description

Technical Field

[0001] This invention relates to the field of livestock and poultry breeding and feed development technology, specifically to the application of lycopene in the preparation of functional feed. Background Technology

[0002] In the development of the livestock and poultry breeding industry, the functional characteristics of feed directly affect the growth, health, and final product quality of livestock and poultry, and are key factors influencing the economic benefits of the breeding industry. Currently, most livestock and poultry feeds on the market have relatively limited functions, and most can only meet the basic nutritional needs of livestock and poultry, making it difficult to play an effective role in key production indicators such as strengthening immunity, improving mobility, and optimizing reproductive efficiency.

[0003] To achieve the goals of disease prevention and promoting livestock and poultry growth, excessive amounts of antibiotics are sometimes added to feeds. This not only leads to antibiotic residues in livestock and poultry products, posing a serious threat to food safety, but also may disrupt the microecological balance within livestock and poultry, hindering the sustainable development of the livestock industry. Furthermore, livestock and poultry generally suffer from insufficient vitality and low reproductive efficiency, further compressing the profit margins of the livestock industry. Therefore, developing a safe, reliable, effective, and versatile functional feed adjuvant has become an urgent need in the current livestock and poultry farming sector.

[0004] Lycopene is a naturally occurring carotenoid widely distributed in various fruits and vegetables such as tomatoes, watermelons, and grapefruits. It possesses excellent antioxidant, anti-inflammatory, and immune-boosting physiological activities. As a natural active ingredient, lycopene has significant advantages such as high safety, good palatability, and no residue, and has been widely used in various fields such as food and health products.

[0005] However, to date, there have been no reports on the use of lycopene in the preparation of functional feeds for livestock and poultry. Its application potential in the field of livestock and poultry farming has not been fully explored, and it cannot provide new technical solutions to the technical defects of existing livestock and poultry feeds.

[0006] Therefore, a new solution is needed to address the above problems. Summary of the Invention

[0007] The purpose of this invention is to provide an application of lycopene in the preparation of functional feed, so as to solve the technical problems mentioned in the background art.

[0008] To achieve the above objectives, the present invention provides the following technical solution: an application of lycopene in the preparation of functional feed, wherein lycopene is used as an auxiliary functional component in the preparation of functional feed, and the functional feed is used at least for livestock and poultry animals.

[0009] Furthermore, the effective addition concentration of lycopene in functional feed is 5 mg / kg / day.

[0010] Furthermore, the auxiliary functions include at least enhancing the immunity of livestock and poultry, promoting reasonable weight gain in livestock and poultry, improving the mobility of livestock and poultry, and increasing the reproductive efficiency of livestock and poultry.

[0011] Furthermore, the functional feed prepared from the lycopene can be applied to silkworm farming.

[0012] Compared with the prior art, the beneficial effects of the present invention are:

[0013] 1. This invention is the first to explore the application value of lycopene in the preparation of functional feeds for livestock and poultry, as well as silkworms, and clarifies that 5 mg / kg / day is its effective addition concentration. At this concentration, lycopene not only has strong physiological activity, but also requires a small amount, which can reduce breeding costs and open up a new path for the large-scale application of lycopene in the field of livestock and poultry breeding.

[0014] 2. This invention discovers that lycopene within its effective concentration range can significantly enhance the immunity of livestock and poultry, improve their resistance to diseases, and reduce the probability of disease occurrence during the breeding process. At the same time, it can also promote reasonable weight gain in livestock and poultry, ensure animal growth performance, and lay a solid foundation for improving breeding efficiency.

[0015] 3. This invention has found that lycopene can significantly enhance the movement ability of livestock and poultry, improve the condition of insufficient vitality in livestock and poultry; and can also improve the reproductive efficiency of livestock and poultry, such as significantly increasing the hatching rate and good egg rate of silkworm eggs, further improving the economic benefits of the breeding industry.

[0016] 4. The lycopene used in this invention is added to functional feed as a natural active ingredient. It has the advantages of good palatability and no residue. Its application in functional feed can replace the use of some antibiotics, fundamentally solving the product safety hazards caused by antibiotic overload. It is in line with the development trend of green farming and has broad market prospects and practical value. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 The effect of lycopene on the body weight of silkworms in their fifth instar.

[0019] Figure 2 The effect of lycopene on the motility of silkworms;

[0020] Figure 3 The effect of lycopene on the reproductive capacity of silkworms. Detailed Implementation

[0021] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0022] Example 1:

[0023] An application of lycopene in the preparation of functional feed, wherein lycopene is used as an auxiliary functional ingredient in the preparation of functional feed, and the functional feed is used for at least livestock and poultry animals.

[0024] The effective addition concentration of lycopene to functional feeds is 5 mg / kg / day.

[0025] The auxiliary functions include at least enhancing the immunity of livestock and poultry, promoting reasonable weight gain in livestock and poultry, enhancing the mobility of livestock and poultry, and improving the reproductive efficiency of livestock and poultry.

[0026] The functional feed prepared from the lycopene can be used in silkworm farming.

[0027] A type of lycopene has the characteristics of low addition amount, good palatability and no residue in the preparation of functional feed for livestock and poultry. It can be used to solve the problems of single function of livestock and poultry feed, product safety hazards caused by antibiotic abuse, and insufficient vitality and low reproductive efficiency of livestock and poultry.

[0028] Example 2:

[0029] Based on the functional feed prepared from lycopene proposed in Example 1, which can be applied to silkworm farming, this example proposes a functional feed prepared from lycopene for silkworm farming, namely, the preparation of mulberry leaves containing lycopene.

[0030] (1) Pretreatment of lycopene raw materials: Select natural lycopene crystals with a purity of ≥98%, prepare lycopene mother liquor with anhydrous ethanol as solvent, stir until completely dissolved, and set aside; at the same time, prepare an equal amount of anhydrous ethanol (for blank control of anhydrous ethanol group).

[0031] (2) Functional feed was formulated based on mulberry leaves, and two experimental feed groups were set up, as follows:

[0032] Control group: Regular mulberry leaves, with only anhydrous ethanol added in the same amount as the lycopene group (10 mL per 1 kg of feed), and no lycopene added;

[0033] 5mg / kg / day group: regular mulberry leaves, with lycopene stock solution added to a final concentration of 5mg / kg;

[0034] Mix each group of mulberry leaves thoroughly with the corresponding solution.

[0035] (3) Experimental grouping: Silkworm larvae of the same age and health status were randomly divided into experimental group and control group, with 60 larvae in each group and 3 replicates. The experimental group was fed functional feed with a final lycopene concentration of 5 mg / kg / day, while the control group was fed control feed.

[0036] (4) Feeding conditions: The feeding environment is controlled to the standard environment for silkworm rearing, ensuring that each silkworm consumes the same amount of food per day (about 0.5g / head / day), and promptly cleaning up residual leaves and feces.

[0037] (5) Index detection and recording, experimental data analysis was performed using GraphPad Prism 9 software for one-way ANOVA (ns, P>0.05; *, P≤0.05; **, P≤0.01; ***, P≤0.001; ****, P≤0.0001).

[0038] See Figure 1 - Figure 3 The experiment of feeding silkworms with mulberry leaves containing lycopene, as proposed in Example 2, is as follows:

[0039] Determination of the effect of lycopene functional feed on the growth performance of silkworms

[0040] Growth performance monitoring: At the peak of the 5th instar (3 days, 4 days, and 5 days), each group of silkworms was weighed using an electronic balance (accuracy 0.001g) and the average weight was calculated.

[0041] The results are as follows: the average weight of the experimental group at 5 years old for 3 days was 0.67g, and that of the control group was 0.70g; the average weight of the experimental group at 5 years old for 4 days was 0.98g, and that of the control group was 0.93g; the average weight of the experimental group at 5 years old for 5 days was 1.60g, and that of the control group was 1.74g.

[0042] The results showed that the average weight of silkworms in the experimental groups at 5 instar 3 days, 5 instar 4 days, and 5 instar 5 days was significantly higher than that in the control group.

[0043] The effect of lycopene-based functional feed on the locomotion ability of silkworms

[0044] Silkworms were divided into two groups based on sex, in addition to the control and experimental groups, and their movement ability was measured.

[0045] (1) Determination of the ability to turn over: Turn the silkworm over and record the time it takes for the silkworm to recover its normal body position. Calculate the average time.

[0046] (2) Determination of grasping ability: Place the silkworm on the brush handle, record the time from when the silkworm is placed on it to when it falls off, and calculate the average time.

[0047] (3) The results are as follows: In the test of flipping ability, the average time for females in the experimental group was 9.77 seconds and the average time for males was 8.72 seconds. In the test of grasping ability, the average time for females in the experimental group was 1.91 seconds and the average time for males was 2.16 seconds. In the test of flipping ability, the average time for females in the control group was 5.38 seconds and the average time for males was 5.53 seconds. In the test of grasping ability, the average time for females in the control group was 4.12 seconds and the average time for males was 3.66 seconds.

[0048] (4) The results showed that the flipping ability of the female experimental group was significantly higher than that of the female control group, the flipping ability of the male experimental group was significantly higher than that of the male control group, the grasping ability of the female experimental group was significantly higher than that of the female control group, and the grasping ability of the male experimental group was significantly higher than that of the male control group.

[0049] Determination of the effects of lycopene-based functional feed on physiological indicators of silkworms

[0050] Reproductive-related indicators:

[0051] (1) Total number of eggs: After the silkworms pupated, the pupae were collected uniformly. After the adults emerged, 10 healthy male moths and 10 healthy female moths were selected from each replicate of each group and mated in a 1:1 ratio. The number of eggs laid by each pair of moths was recorded, and all silkworm eggs in the sample were counted (excluding eggs that were completely broken or had only empty shells). The results are as follows: the total number of eggs in the experimental group was 12,567, and the total number of eggs in the control group was 12,948.

[0052] (2) Good egg rate: Each defective egg (unfertilized egg, dead egg, deformed egg, contaminated egg) was picked out, and the good egg rate was calculated (good egg rate = (total number of eggs - number of defective eggs) / total number of eggs × 100%). The results are as follows: the average good egg rate of the experimental group was 97.2%, and the average good egg rate of the control group was 98.0%;

[0053] (3) Hatching rate: The silkworm eggs were placed at 25℃ and 70% humidity to induce hatching. After 7 days, the hatching rate was calculated (hatching rate = number of hatched silkworms / total number of eggs × 100%). The results are as follows: the average hatching rate of the experimental group was 96.8%, and the average hatching rate of the control group was 95.3%.

[0054] (4) The results showed that the total number of silkworm eggs in the experimental group was not significantly better than that in the control group, the hatching rate was significantly higher than that in the control group, and the rate of good eggs was significantly higher than that in the control group.

[0055] Based on the above indicators, a lycopene concentration of 5 mg / kg / day provides good palatability for silkworms and can significantly improve their growth performance, immunity, and reproductive efficiency.

[0056] In summary:

[0057] This invention reveals that when lycopene is added at an effective concentration of 5 mg / kg / day to silkworms, it can effectively enhance the silkworms' immunity, promote reasonable weight gain, significantly improve their mobility, and significantly increase the hatching rate and quality rate of silkworm eggs. Therefore, lycopene has significant potential as an auxiliary functional ingredient in the preparation of functional feeds for livestock and poultry. Its advantages of low addition amount, good palatability, and no residue provide a new direction for the expanded application of lycopene in the field of livestock and poultry farming. It can also provide strategies and solutions to effectively address current problems such as the single function of livestock and poultry feed, the potential for product effectiveness risks due to antibiotic abuse, and insufficient vitality and low reproductive efficiency in livestock and poultry.

[0058] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. An application of lycopene in the preparation of functional feed, characterized in that: Lycopene is used as an auxiliary functional ingredient in the preparation of functional feed, which is used at least in livestock and poultry animals.

2. The application of lycopene in the preparation of functional feed according to claim 1, characterized in that: The effective addition concentration of lycopene to functional feed is 5 mg / kg / day.

3. The application of lycopene in the preparation of functional feed according to claim 1, characterized in that: The auxiliary functions include at least enhancing the immunity of livestock and poultry, promoting reasonable weight gain in livestock and poultry, improving the mobility of livestock and poultry, and increasing the reproductive efficiency of livestock and poultry.

4. The application of lycopene in the preparation of functional feed according to claim 1, characterized in that: The functional feed prepared from the lycopene can be used in silkworm farming.

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