Pet halitosis-removing freeze-dried powder containing compound enzyme and preparation method of freeze-dried powder

Abstract

The invention provides a compound enzyme-containing freeze-dried powder for removing halitosis of pets and a preparation method of the freeze-dried powder. The compound enzyme-containing freeze-dried powder for removing halitosis of pets comprises the following components in parts by weight: 60-90 parts of chicken, 0.5-5 parts of an intestinal regulator, 0.1-5 parts of an anti-inflammatory antioxidant, 0.1-3 parts of compound enzyme and 0.1-3 parts of an additive, the compound enzyme comprises a combination of at least two of papain, egg white lysozyme, glucose oxidase or lactoperoxidase. The pet halitosis-removing freeze-dried powder containing the compound enzyme can cover local cleaning of the oral cavity, inflammation improvement and environmental regulation in the intestinal tract, namely, comprehensive halitosis removal can be achieved for oral cavity health and intestinal tract health, all the used components are natural components, health risks possibly brought by chemical components are avoided, long-term health of pets can be guaranteed, and the pet halitosis-removing freeze-dried powder containing the compound enzyme is safe and reliable. And the safety is high.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of pet oral health care, and particularly relates to a pet oral odor-removing freeze-dried product containing composite enzymes and a preparation method thereof. BACKGROUND

[0002] Due to the fact that pets rarely clean their oral cavities, a large amount of food residues in the oral cavities are accumulated for a long time, and are fermented by anaerobic bacteria to form dental plaque, which is calcified with saliva minerals to form dental calculus and produce oral odor; the flora in the intestines is disordered, leading to a large number of Helicobacter pylori to reproduce and produce oral odor. With the youth of pet owners, the importance of pet oral health is gradually increasing, but dogs and cats do not have the habit of brushing their teeth and resist tooth brushing, and it is easy to cause the gums of dogs and cats to become red and swollen, gingivitis and the like when brushing their teeth. Products related to pet oral care have been launched on the market, such as toothpicks, mouthwashes and sprays, to cope with pet oral health in various situations.

[0003] CN114208964A discloses a pet tooth cleaning bone with oral cleaning function, and the raw materials include: rice flour 25-45%, chicken 8-22%, sweet potato starch 10-20%, pea starch 5-20%, malt dextrin 4-10%, glycerol 4-15%, calcium carbonate 0.5-5%, vegetable oil 0.2-2%, casein phosphopeptide 0.1-2%, sodium hexametaphosphate 0.1-2%, baking soda 0.1-1%, chlorogenic acid 0.05-2%, tea polyphenol 0.05-2%, magnolol extract 0.05-2%, protease 0.05-1%, dextranase 0.05-1%, vitamin E 0.01-0.1%. The present scheme can solve the technical problems of the pet food in the prior art, such as unsatisfactory pet tooth cleaning effect and large side effects, and can be widely applied to the practical operation of pet feeding and oral cleaning.

[0004] CN112315842A discloses a dog and cat oral care spray containing lysozyme and a preparation method thereof. The dog and cat oral care spray contains the following raw materials in mass fractions: egg white lysozyme 0.35-0.75 parts, persimmon tannin 0.5-1 part, honeysuckle extract 8-15 parts, sophora 0.45-1 part, corallium 10-15 parts, licorice 10-15 parts, xylitol 1-3 parts, chitosan oligosaccharide 0.1-0.5 parts, polyphosphate 0.5-1 part, pet flavor enhancer 0.05-0.3 part, and water 30-50 parts. The lysozyme accounts for not more than 0.75% of the total mass of the oral care composition spray, and the persimmon tannin accounts for not more than 1% of the total mass of the spray.

[0005] However, current products often suffer from slow effectiveness and only address the symptoms, not the root cause. Furthermore, long-term use of most of these products may pose potential health risks to pets. Therefore, there is a need for a safe and effective product that can prevent or eliminate pet halitosis by addressing both oral and intestinal health to meet consumer demand. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the present invention aims to provide a freeze-dried pet product containing a complex enzyme for eliminating bad breath, and its preparation method. The freeze-dried pet product containing the complex enzyme for eliminating bad breath of the present invention can be fed directly without the need for brushing teeth, making it convenient and simple to operate. Furthermore, the freeze-dried product contains natural ingredients, is easy to feed, and avoids the risk of pet owners being scratched.

[0007] To achieve this objective, the present invention employs the following technical solution:

[0008] In a first aspect, the present invention provides a pet halitosis-removing freeze-dried product containing a complex enzyme, wherein the pet halitosis-removing freeze-dried product containing a complex enzyme comprises the following components by weight: 60-90 parts chicken meat, 0.5-5 parts intestinal regulator, 0.1-5 parts anti-inflammatory and antioxidant agent, and 0.1-3 parts complex enzyme;

[0009] The complex enzyme comprises a combination of at least two of papain, egg white lysozyme, glucose oxidase, or lactoperoxidase.

[0010] The freeze-dried pet product containing compound enzymes of this invention can decompose the core substances in the oral cavity that cause halitosis, including food debris, shed epithelial cells, and bacterial metabolites, reducing the nutrient source for bacterial growth and cleaning hard-to-reach areas such as between teeth and gingival sulcus. Anti-inflammatory and antioxidant agents can improve oral inflammation and oxidative stress, alleviating inflammatory responses such as gingival redness and swelling and periodontitis, which are important causes of halitosis. They also clear free radicals in the oral cavity, reducing oxidative damage to the gums and oral mucosa, and maintaining a healthy oral environment. Intestinal regulators can regulate the balance of intestinal flora, reduce the proliferation of harmful bacteria in the intestines, improve the pet's digestive and absorptive functions, reduce the fermentation of undigested food in the intestines, and prevent odor from refluxing through the respiratory tract and causing halitosis. The combination of these three components covers local oral cleaning, inflammation improvement, and intestinal environment regulation, thus achieving comprehensive halitosis elimination targeting both oral and intestinal health.

[0011] The amount of chicken added in the freeze-dried pet breath deodorizer containing compound enzymes of the present invention can be 62 parts, 65 parts, 68 parts, 70 parts, 72 parts, 75 parts, 78 parts, 80 parts, 82 parts, 85 parts, or 88 parts, etc.

[0012] The amount of the intestinal regulator can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, or 4.5 parts, etc.;

[0013] The amount of the anti-inflammatory and antioxidant agent added can be 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, or 4.5 parts, etc.;

[0014] The amount of the compound enzyme added can be 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, or 2.8 parts, etc.

[0015] Preferably, the chicken meat includes chicken breast and / or chicken thigh meat.

[0016] Preferably, the intestinal regulator comprises any one or a combination of at least two of spinach extract, mulberry leaf extract, yucca extract, or purslane extract.

[0017] Preferably, the intestinal regulator is a combination of spinach extract, mulberry leaf extract, yucca extract and purslane extract.

[0018] Preferably, the mass ratio of the spinach extract, mulberry leaf extract, yucca extract and purslane extract is (1-3):(0.1-1):(0.5-1.5):(1-2).

[0019] The values ​​“1-3” can be 1.1, 1.3, 1.5, 1.8, 2, 2.2, 2.5, 2.7, or 2.9, etc.

[0020] "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, or 0.9, etc.;

[0021] "0.5-1.5" can be 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3 or 1.4, etc.;

[0022] “1-2” can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.

[0023] Preferably, the anti-inflammatory and antioxidant agent includes any one or a combination of at least two of the following: spirulina extract, centella asiatica extract, or wild chrysanthemum extract.

[0024] Preferably, the anti-inflammatory and antioxidant agent is a combination of spirulina extract, centella asiatica extract and wild chrysanthemum extract.

[0025] Preferably, the mass ratio of the spirulina extract, centella asiatica extract and wild chrysanthemum extract is (1-2):(3-5):(2-3).

[0026] "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, or 1.9, etc.

[0027] “3-5” can be 3.1, 3.3, 3.5, 3.8, 4, 4.1, 4.3, 4.5, 4.7 or 4.9, etc.;

[0028] “2-3” can be 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8 or 2.9, etc.

[0029] Preferably, the complex enzyme is a combination of papain, egg white lysozyme, glucose oxidase and lactoperoxidase.

[0030] Preferably, the mass ratio of papain, egg white lysozyme, glucose oxidase and lactoperoxidase is (2-5):(1-2):(2-3):(1-2).

[0031] Where “2-5” can be 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5 or 4.8, etc.;

[0032] The first "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, or 1.9, etc.;

[0033] “2-3” can be 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, or 2.9, etc.;

[0034] The second "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.

[0035] Preferably, the freeze-dried pet deodorant containing compound enzymes further includes 0.1-3 parts by weight of additives, such as 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, or 2.8 parts, etc.

[0036] Preferably, the additive includes any one or a combination of at least two of the following: tea polyphenols, fructooligosaccharides, mannose, vitamin E, vitamin B2, vitamin B1, vitamin B6, or vitamin B12.

[0037] Preferably, the spinach extract, mulberry leaf extract, yucca extract, purslane extract, spirulina extract, centella asiatica extract, and wild chrysanthemum extract used in this invention can all be extracted using conventional water extraction or alcohol extraction methods.

[0038] An exemplary method for extracting spinach extract includes:

[0039] After drying and pulverizing the spinach, heat it to boiling using a 60-70% (v / v) ethanol aqueous solution, reflux and extract the extract, concentrate and dry it to obtain the final product.

[0040] The extraction methods for the mulberry leaf extract, yucca extract, purslane extract, spirulina extract, centella asiatica extract, and wild chrysanthemum extract can be performed in accordance with the methods described above.

[0041] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0042] In a second aspect, the present invention provides a method for preparing a pet halitosis-removing freeze-dried product containing a complex enzyme according to the first aspect, the method comprising pulverizing chicken meat and mixing it with an intestinal regulator, an anti-inflammatory antioxidant, and a complex enzyme, and then freeze-drying it to obtain the pet halitosis-removing freeze-dried product containing the complex enzyme.

[0043] Preferably, the preparation method includes:

[0044] (1) After the chicken is cooked, it is shredded and then heated at a constant temperature in a constant temperature water bath.

[0045] A mixture is obtained by combining anti-inflammatory and antioxidant agents, intestinal regulators, and complex enzymes.

[0046] (2) Add the mixture to a constant temperature water bath and mix it with the chicken to obtain a mixed meat paste;

[0047] (3) The mixed meat paste was freeze-dried to obtain the pet deodorizing freeze-dried product containing the complex enzyme.

[0048] Preferably, the preparation of the mixture in step (1) further includes the addition of additives.

[0049] Preferably, the temperature of the constant temperature heating in step (1) is 35-40℃ (for example, it can be 36℃, 37℃, 38℃ or 39℃, etc.), and the time is 1.5-3h (for example, it can be 1.6h, 1.8h, 2h, 2.2h, 2.4h, 2.6h or 2.8h, etc.).

[0050] Preferably, the freeze-drying step (3) is as follows: pre-freezing at -55~-65℃ (e.g., -56℃, -58℃, -60℃, -62℃ or -64℃, etc.) for 7-10 hours (e.g., 7.5 hours, 8 hours, 8.5 hours, 9 hours or 9.5 hours, etc.), and freeze-drying at a vacuum of 90-100Pa (e.g., 91 Pa, 93 Pa, 95 Pa, 97 Pa or 99 Pa, etc.) at -5~-10℃ (e.g., -6℃, -7℃, -8℃ or -9℃, etc.) for 20-30 hours (e.g., 21 hours, 23 hours, 25 hours, 27 hours or 29 hours, etc.).

[0051] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0052] Compared with the prior art, the present invention has the following beneficial effects:

[0053] (1) The compound enzymes in the freeze-dried pet deodorant of the present invention can decompose the core substances in the oral cavity that cause halitosis, including food residue, exfoliated epithelial cells and bacterial metabolites, reducing the nutrient source for bacterial growth, and cleaning dead corners such as tooth gaps and gingival sulci; the anti-inflammatory and antioxidant agents can improve oral inflammation and oxidative stress, relieve inflammatory reactions such as gingival redness and swelling and periodontitis, and inflammation is an important cause of halitosis. At the same time, they can remove free radicals in the oral cavity, reduce oxidative damage to the gums and oral mucosa, and maintain a healthy oral environment; the intestinal regulator can regulate the balance of intestinal flora, reduce the proliferation of harmful bacteria in the intestine, improve the pet's digestive and absorptive functions, reduce the fermentation of undigested food in the intestine, and prevent odor from refluxing through the respiratory tract to form halitosis. The combination of the three can cover local oral cleaning, inflammation improvement and intestinal environment regulation, that is, it can achieve all-round halitosis removal for both oral health and intestinal health.

[0054] (2) The intestinal regulators with natural antibacterial and protective properties of the intestinal mucosa added in this invention, namely spinach extract, mulberry leaf extract, yucca extract and purslane extract, can protect the intestinal mucosal barrier, regulate the intestinal flora structure and reduce the risk of intestinal inflammation.

[0055] (3) The anti-inflammatory and antioxidant agents with natural anti-inflammatory properties added in this invention, namely Spirulina extract, Centella asiatica extract, and Chrysanthemum indicum extract, can regulate the immune system, inhibit the release of inflammatory factors, and enhance the cellular antioxidant defense system.

[0056] (4) The glucose oxidase and lactoperoxidase added in this invention can work together to inhibit oral pathogens, reduce dental plaque and halitosis; the added egg white lysozyme can destroy the bacterial cell wall and dissolve dental plaque; the added papain can decompose oral debris and reduce the substrate for bacterial odor production. The four work together to enhance the effect of the product in eliminating halitosis.

[0057] (5) The pet deodorizing freeze-dried pet containing compound enzymes prepared by this invention is based on natural ingredients, avoiding the health risks that chemical ingredients may bring, and can ensure the long-term health of pets with high safety. Detailed Implementation

[0058] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0059] The sources of some of the raw materials used in the embodiments and comparative examples of this invention are as follows:

[0060]

[0061] All other raw materials can be used as long as they are purchased from authorized distributors.

[0062] Example 1

[0063] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme, wherein the pet halitosis-removing freeze-dried product containing the compound enzyme comprises, by weight, 79 parts chicken breast, 3 parts intestinal regulator, 3 parts anti-inflammatory and antioxidant, 2 parts compound enzyme, and 2 parts additives;

[0064] The anti-inflammatory and antioxidant agent mentioned above is a combination of Spirulina extract, Centella asiatica extract and Chrysanthemum indicum extract in a mass ratio of 1.5:4:2.5;

[0065] The intestinal regulator is a combination of spinach extract, mulberry leaf extract, yucca extract and purslane extract in a mass ratio of 2:0.5:1:1.5.

[0066] The complex enzyme is a combination of papain, egg white lysozyme, glucose oxidase and lactoperoxidase in a mass ratio of 4:1.5:2.5:1.5.

[0067] The additives are fructooligosaccharides, vitamin E, and vitamin B2 in a mass ratio of 1:1:1.

[0068] The preparation method of the freeze-dried pet product containing compound enzymes to eliminate bad breath is as follows:

[0069] (1) Cook the chicken breast in boiling water until cooked through, let it cool naturally to room temperature, grind it into a uniform powder in a meat grinder, add water and place it in a constant temperature water bath. Set the temperature of the constant temperature water bath to 37°C and bathe in the constant temperature water bath for 2 hours.

[0070] (2) Add the anti-inflammatory antioxidant, intestinal regulator, additive, and compound enzyme to an empty beaker, stir well, then add the additive and stir until completely dissolved to obtain a mixture;

[0071] (3) Add the mixture in small amounts several times to the constant temperature water bath in step (1) and stir evenly to obtain mixed meat paste;

[0072] (4) Pour the mixed meat paste into the mold, pre-freeze it at -60℃ for 8 hours, and then freeze-dry it in a freeze dryer at a vacuum of 95Pa and a temperature of -10℃ for 24 hours. After demolding, you will get the pet breath freshener freeze-dried product containing compound enzymes.

[0073] Example 2

[0074] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme, which, by weight, comprises 84 parts chicken leg meat, 1 part intestinal regulator, 2 parts anti-inflammatory and antioxidant agent, 1 part compound enzyme, and 2 parts additives (vitamin E and vitamin B2).

[0075] The anti-inflammatory and antioxidant agent mentioned therein is a combination of Spirulina extract, Centella asiatica extract and Chrysanthemum indicum extract in a mass ratio of 2:3:3;

[0076] The intestinal regulator is a combination of spinach extract, mulberry leaf extract, yucca extract and purslane extract in a mass ratio of 1:1:0.5:2.

[0077] The complex enzyme is a combination of papain, egg white lysozyme, glucose oxidase and lactoperoxidase in a mass ratio of 5:1:3:1.

[0078] The additives are mannose, vitamin E, and vitamin B2 in a mass ratio of 1:1:1.

[0079] The preparation method of the freeze-dried pet product containing compound enzymes to eliminate bad breath is as follows:

[0080] (1) Cook the chicken breast in boiling water until cooked through, let it cool naturally to room temperature, grind it into a uniform powder in a meat grinder, add water and place it in a constant temperature water bath. Set the temperature of the constant temperature water bath to 40°C and bathe in the constant temperature water bath for 1.5 hours.

[0081] (2) Add the anti-inflammatory antioxidant, intestinal regulator, additive, and compound enzyme to an empty beaker, stir well, then add the additive and stir until completely dissolved to obtain a mixture;

[0082] (3) Add the mixture in small amounts several times to the constant temperature water bath in step (1) and stir evenly to obtain mixed meat paste;

[0083] (4) Pour the mixed meat paste into the mold, pre-freeze it at -65℃ for 7 hours, then place it in a freeze dryer and freeze dry it at a vacuum of 90Pa and a temperature of -5℃ for 30 hours. After demolding, you will get the pet breath deodorant freeze-dried product containing compound enzymes.

[0084] Example 3

[0085] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme, wherein the pet halitosis-removing freeze-dried product containing the compound enzyme comprises, by weight, 75 parts chicken breast, 5 parts intestinal regulator, 4 parts anti-inflammatory and antioxidant, 3 parts compound enzyme, and 3 parts additives.

[0086] The anti-inflammatory and antioxidant agent mentioned above is a combination of Spirulina extract, Centella asiatica extract and Chrysanthemum indicum extract in a mass ratio of 1:5:2;

[0087] The intestinal regulator is a combination of spinach extract, mulberry leaf extract, yucca extract and purslane extract in a mass ratio of 3:0.3:1.5:1.

[0088] The complex enzyme is a combination of papain, egg white lysozyme, glucose oxidase and lactoperoxidase in a mass ratio of 1:1:1:1.

[0089] The additives are tea polyphenols, vitamin E, and vitamin B2 in a mass ratio of 1:1:1.

[0090] The preparation method of the freeze-dried pet product containing compound enzymes to eliminate bad breath is as follows:

[0091] (1) Cook the chicken breast in boiling water until cooked through, let it cool naturally to room temperature, grind it into a uniform powder in a meat grinder, add water and place it in a constant temperature water bath. Set the temperature of the constant temperature water bath to 35°C and bathe in the constant temperature water bath for 3 hours.

[0092] (2) Add the anti-inflammatory antioxidant, intestinal regulator, additive, and compound enzyme to an empty beaker, stir well, then add the additive and stir until completely dissolved to obtain a mixture;

[0093] (3) Add the mixture in small amounts several times to the constant temperature water bath in step (1) and stir evenly to obtain mixed meat paste;

[0094] (4) Pour the mixed meat paste into the mold, pre-freeze it at -55℃ for 10 hours, then place it in a freeze dryer and freeze dry it at a vacuum of 100Pa and a temperature of -8℃ for 20 hours. After demolding, you will get the pet breath freshener containing compound enzymes.

[0095] Example 4

[0096] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the compound enzyme is a combination of papain, egg white lysozyme, and glucose oxidase in a mass ratio of 4:1.5:2.5, and the amount of the compound enzyme remains unchanged. The other components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0097] Example 5

[0098] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the compound enzyme is a combination of papain, egg white lysozyme, and lactoperoxidase in a mass ratio of 4:1.5:1.5, and the amount of the compound enzyme remains unchanged. The other components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0099] Example 6

[0100] This embodiment provides a pet halitosis-removing freeze-dried product containing a complex enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the complex enzyme is a combination of papain, glucose oxidase and lactoperoxidase in a mass ratio of 4:2.5:1.5, and the amount of the complex enzyme remains unchanged. The other components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0101] Example 7

[0102] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the compound enzyme is a combination of egg white lysozyme, glucose oxidase and lactoperoxidase in a mass ratio of 3:5:3, and the amount of the compound enzyme remains unchanged. The other components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0103] Example 8

[0104] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the compound enzyme is a combination of papain, egg white lysozyme, glucose oxidase and lactoperoxidase in a mass ratio of 6:0.5:4:0.5, and the amount of the compound enzyme remains unchanged. The other components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0105] Example 9

[0106] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between the pet halitosis-removing freeze-dried product containing the compound enzyme and that in Example 1 is that the compound enzyme is a combination of papain, egg white lysozyme, glucose oxidase and lactoperoxidase in a mass ratio of 1:3:1:3, and the amount of the compound enzyme remains unchanged. The other components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0107] Example 10

[0108] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the anti-inflammatory and antioxidant agent is a combination of spirulina extract and centella asiatica extract in a mass ratio of 1.5:4, and the amount of anti-inflammatory and antioxidant agent remains unchanged. The other components and their amounts remain unchanged, and the preparation method is the same as in Example 1.

[0109] Example 11

[0110] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the anti-inflammatory and antioxidant agent is a combination of spirulina extract and wild chrysanthemum extract in a mass ratio of 1.5:2.5, and the amount of anti-inflammatory and antioxidant agent remains unchanged. The other components and their amounts remain unchanged, and the preparation method is the same as in Example 1.

[0111] Example 12

[0112] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the anti-inflammatory and antioxidant agent is a combination of Centella asiatica extract and Chrysanthemum indicum extract in a mass ratio of 4:2.5, and the amount of anti-inflammatory and antioxidant agent remains unchanged. The other components and their amounts remain unchanged, and the preparation method is the same as in Example 1.

[0113] Example 13

[0114] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the intestinal regulator is a combination of spinach extract, mulberry leaf extract and yucca extract in a mass ratio of 2:0.5:1, and the amount of the intestinal regulator remains unchanged. The other components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0115] Example 14

[0116] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the intestinal regulator is a combination of spinach extract, mulberry leaf extract and purslane extract in a mass ratio of 2:0.5:1.5, and the amount of the intestinal regulator remains unchanged. The other components and their amounts remain unchanged, and the preparation method is the same as in Example 1.

[0117] Example 15

[0118] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the intestinal regulator is a combination of spinach extract, yucca extract and purslane extract in a mass ratio of 2:1:1.5, and the amount of the intestinal regulator remains unchanged. The other components and their amounts remain unchanged, and the preparation method is the same as in Example 1.

[0119] Example 16

[0120] This embodiment provides a pet halitosis-removing freeze-dried product containing a compound enzyme. The only difference between this pet halitosis-removing freeze-dried product and that in Example 1 is that the intestinal regulator is a combination of mulberry leaf extract, yucca extract, and purslane extract in a mass ratio of 0.5:1:1.5, and the amount of the intestinal regulator remains unchanged. The other components and their amounts remain unchanged, and the preparation method is the same as in Example 1.

[0121] Comparative Example 1

[0122] This comparative example provides a pet halitosis-removing freeze-dried product. The only difference between the pet halitosis-removing freeze-dried product containing compound enzymes and Example 1 is that it does not contain compound enzymes. The reduction in the amount of compound enzymes is made up by intestinal regulators and anti-inflammatory and antioxidant agents in proportion to the parts. The remaining components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0123] Comparative Example 2

[0124] This comparative example provides a pet halitosis-removing freeze-dried product containing a complex enzyme. The only difference between the pet halitosis-removing freeze-dried product containing the complex enzyme and Example 1 is that it does not include an intestinal regulator. The reduction in this amount is made up by the complex enzyme and anti-inflammatory and antioxidant agents in proportion to the parts. The remaining components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0125] Comparative Example 3

[0126] This comparative example provides a pet halitosis-removing freeze-dried product containing a complex enzyme. The only difference between this pet halitosis-removing freeze-dried product and Example 1 is that it does not contain anti-inflammatory and antioxidant agents. The reduction in these agents is made up by the complex enzyme and intestinal regulator in proportion to the parts. The remaining components and their amounts remain unchanged. The preparation method is the same as in Example 1.

[0127] Test case

[0128] Experimental Methods: One hundred cats were divided into groups of five according to the experimental design. Each group was fed the products of Examples 1-16 and Comparative Examples 1-3, as well as chicken pellets (blank control), and was independently housed and managed. Adequate water access was ensured for all animals during the experiment, and feeding was performed according to the experimental design. The housing environment was kept clean and hygienic. Breath odor, dental plaque, and antibacterial effects in saliva were measured in each group on day 0 (before the experiment) and day 14.

[0129] (1) Determination of volatile sulfur compounds (breath value) in the oral cavity

[0130] The oral cavity of the test pet was kept closed, and gas was collected from the oral cavity using an airtight syringe. The gas was then injected into the breathalyzer at a constant rate to measure the concentration of volatile sulfur compounds (VSCs) in the breath. Each test cat was measured three times and the average value was taken.

[0131] (2) Dental plaque measurement

[0132] For each test cat, three teeth with the most severe plaque were examined, and the plaque size was recorded. The changes and proportions of plaque size before and after the test were compared between the test groups.

[0133] (3) Determination of the antibacterial effect of saliva

[0134] Saliva was collected from a fixed area inside the mouth of the test cats using a sterile cotton swab. The sample was then placed in a sterile centrifuge tube containing 3 ml of sterile physiological saline, shaken thoroughly, and used to soak sterile filter paper. An appropriate amount of Streptococcus mutans working bacterial suspension was evenly spread on the surface of BHI medium. The filter paper containing saliva was then placed on the surface of the medium inoculated with Streptococcus mutans. The plate was inverted and incubated in a 37°C anaerobic incubator for 18 hours. After incubation, the formation of a sterile inhibition zone around the filter paper was observed, and the diameter of the inhibition zone was measured.

[0135] The results are shown in Table 1.

[0136] Table 1

[0137]

[0138] Note: Saliva antibacterial enhancement rate = (14d inhibition zone diameter - 0d inhibition zone diameter) / 0d inhibition zone diameter

[0139] Results Analysis: As shown in Table 1, compared with the blank control, the pet halitosis-reducing freeze-dried products containing compound enzymes in Examples 1-3 of this invention can effectively reduce the halitosis value of pets, with a reduction rate of more than 55%, a plaque reduction rate of more than 12%, and a saliva antibacterial enhancement rate of more than 35%, indicating that the products of this invention have a significant effect on eliminating halitosis in pets.

[0140] As can be seen from Examples 4-9, the compound enzyme combination selected in this invention has a synergistic effect in reducing dental plaque and halitosis, and improving the antibacterial rate of saliva. All four are indispensable, and the product's halitosis-eliminating effect can only be more significant when the ratio of the four is within the limits of this invention.

[0141] As can be seen from Examples 10-16, the selection of the anti-inflammatory antioxidant and intestinal regulator components also affects the product's halitosis-eliminating effect;

[0142] As shown in Comparative Example 1, when any one of the compound enzyme, anti-inflammatory antioxidant, and intestinal regulator is missing, the plaque and bad breath values ​​of the kittens after consuming the product of this invention are significantly increased, and the antibacterial rate of saliva is significantly reduced. This indicates that the three also have a synergistic effect on the above-mentioned effects, and the combination of the three can make the product more effective.

[0143] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0144] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0145] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A freeze-dried pet product containing a complex enzyme to eliminate bad breath, characterized in that, The freeze-dried pet product containing compound enzymes for eliminating bad breath comprises the following components by weight: 60-90 parts chicken, 0.5-5 parts intestinal regulator, 0.1-5 parts anti-inflammatory and antioxidant, and 0.1-3 parts compound enzymes; The complex enzyme comprises a combination of at least two of papain, egg white lysozyme, glucose oxidase, or lactoperoxidase.

2. The freeze-dried pet deodorant containing compound enzymes according to claim 1, characterized in that, The chicken meat includes chicken breast and / or chicken thigh meat; Preferably, the intestinal regulator comprises any one or a combination of at least two of spinach extract, mulberry leaf extract, yucca extract, or purslane extract.

3. The pet halitosis deodorizing freeze-dried product containing compound enzymes according to claim 2, characterized in that, The intestinal regulator is a combination of spinach extract, mulberry leaf extract, yucca extract and purslane extract.

4. The freeze-dried pet deodorant containing a complex enzyme according to any one of claims 1-3, characterized in that, The anti-inflammatory and antioxidant agents include any one or a combination of at least two of the following: spirulina extract, centella asiatica extract, or wild chrysanthemum extract.

5. The pet halitosis-eliminating freeze-dried product containing compound enzymes according to claim 4, characterized in that, The anti-inflammatory and antioxidant agent is a combination of spirulina extract, centella asiatica extract, and wild chrysanthemum extract.

6. The freeze-dried pet deodorant containing a complex enzyme according to any one of claims 1-5, characterized in that, The complex enzyme is a combination of papain, egg white lysozyme, glucose oxidase and lactoperoxidase.

7. The pet halitosis-eliminating freeze-dried product containing a complex enzyme according to claim 6, characterized in that, The mass ratio of papain, egg white lysozyme, glucose oxidase and lactoperoxidase is (2-5):(1-2):(2-3):(1-2).

8. The freeze-dried pet deodorant containing a complex enzyme according to any one of claims 1-7, characterized in that, The freeze-dried pet product containing compound enzymes for eliminating bad breath also includes 0.1-3 parts by weight of additives; Preferably, the additive includes any one or a combination of at least two of the following: tea polyphenols, fructooligosaccharides, mannose, vitamin E, vitamin B2, vitamin B1, vitamin B6, or vitamin B12.

9. A method for preparing a pet deodorizing freeze-dried product containing a complex enzyme according to any one of claims 1-8, characterized in that, The preparation method includes pulverizing chicken meat and mixing it with intestinal regulators, anti-inflammatory and antioxidant agents, and compound enzymes, followed by freeze-drying to obtain the freeze-dried pet odor-eliminating compound enzyme.

10. The method for preparing freeze-dried pet deodorant containing a complex enzyme according to claim 9, characterized in that, The preparation method includes: (1) After the chicken is cooked, it is shredded and then heated at a constant temperature in a constant temperature water bath. A mixture is obtained by combining anti-inflammatory and antioxidant agents, intestinal regulators, and complex enzymes. (2) Add the mixture to a constant temperature water bath and mix it with the chicken to obtain a mixed meat paste; (3) The mixed meat paste was freeze-dried to obtain the pet deodorizing freeze-dried product containing the complex enzyme; Preferably, the preparation of the mixture in step (1) further includes the addition of additives; Preferably, the constant temperature heating in step (1) is 35-40℃ and the time is 1.5-3h; Preferably, the freeze-drying step (3) is as follows: pre-freezing at -55~-65℃ for 7-10h, and freeze-drying at -5~-10℃ under a vacuum of 90-100Pa for 20-30h.

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