Construction method and application of high-sensitivity fluorescent probe for rapidly analyzing acrylamide in high-temperature processed food
By constructing NAS-CDs/Eu-MOFs fluorescent probes, the complexity and low efficiency of acrylamide detection in high-temperature processed foods were solved, achieving rapid, sensitive, and reliable detection results, suitable for smartphone detection platforms.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-31
- Publication Date
- 2026-04-10
AI Technical Summary
Existing methods for detecting acrylamide in high-temperature processed foods suffer from drawbacks such as expensive instruments, complex operation, long detection time, and low reproducibility, making it difficult to achieve rapid and accurate detection.
A highly sensitive fluorescent probe based on NAS-CDs/Eu-MOFs was constructed by grafting acrylate-N-succinimide onto carbon dots and growing it in situ into a europium-based fluorescent metal-organic framework to form a ratiometric fluorescent probe, which was then used for detection in conjunction with a smartphone.
This method enables rapid and sensitive detection of acrylamide in high-temperature processed foods, reduces interference from external factors, improves the reliability of analytical results, and enhances practical applicability through visualization.
Smart Images
Figure CN121825535A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the technical field of preparation and rapid detection of composite materials, and particularly relates to a high-sensitivity fluorescent probe construction method for rapid analysis of acrylamide in high-temperature processed food and application. BACKGROUND
[0002] High-temperature processed food refers to food prepared through cooking, baking, frying and other processing processes under high-temperature conditions. These processing processes can change the taste, aroma and appearance of food, but can also produce some potential hazards, among which acrylamide (AM) is one of the most concerned substances. Since it was first discovered in high-temperature processed food by Swedish scientists in 2002, its food safety problem has attracted global attention. This substance is mainly generated by Maillard reaction of asparagine and reducing sugar in food at a high temperature of more than 120℃, and is commonly found in bread, potato chips and biscuits. The harm of acrylamide to human health mainly lies in its neurotoxicity, genotoxicity and carcinogenicity. Animal experiments show that long-term high-dose exposure to acrylamide can cause damage to the nervous system and increase the risk of tumors in organs such as thyroid, adrenal gland and breast. Although there is no direct evidence of carcinogenicity in human epidemiological studies, the International Agency for Research on Cancer has listed it as a 2A class carcinogen (i.e. possibly carcinogenic to humans). Therefore, it is crucial to accurately and rapidly detect acrylamide in food.
[0003] Nowadays, the quantitative analysis methods of acrylamide in food include liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, surface-enhanced Raman spectroscopy, electrochemical method, etc. Although these acrylamide analysis methods have achieved satisfactory results, there are still some shortcomings such as expensive instruments, complex operation, long detection time and low reproducibility. In recent years, rapid detection methods for acrylamide have developed rapidly, among which the fluorescence analysis method has attracted much attention due to its high sensitivity, simple operation and fast detection speed.
[0004] In the field of instant detection, smartphones, as high-performance detection devices integrating all components required by general analytical laboratories, are well known to the public due to their simple operation, small size, fast processing speed and high resolution. Smartphones can detect and analyze signals through camera transduction and installed application programs, and display results on the screen, providing a cost-effective and portable sensing platform for on-site detection. SUMMARY
[0005] The present application provides a high-sensitivity fluorescent probe for rapid analysis of acrylamide in high-temperature processed food. In the present application, acrylamide-N-succinimidyl ester (NAS) is grafted on carbon dots (CDs) and grown in situ into europium-based fluorescent metal organic frameworks (Eu-MOF) to construct a ratio-type fluorescent probe for high-sensitivity detection.
[0006] The present application first provides a high-sensitivity fluorescent probe for rapid analysis of acrylamide in high-temperature processed food, which is based on the construction of NAS-CDs / Eu-MOFs.
[0007] A method for constructing a high-sensitivity fluorescent probe for rapid analysis of acrylamide in high-temperature processed food, comprising the following steps: S1: Preparation of NAS-CDs: Mix citric acid and ethylenediamine, then add ultrapure water, transfer the obtained mixed solution to a high-pressure reaction kettle for reaction, after the reaction is completed, wait for natural cooling to room temperature, then filter with a water-phase microporous filter membrane, dilute with PBS buffer solution, and add acrydite solution, and stir under light-proof conditions to obtain NAS-CDs. S2: Preparation of NAS-CDs / Eu-MOFs probe: Dissolve europium nitrate hexahydrate (Eu(NO3)3·6H2O) in ultrapure water to obtain a europium nitrate hexahydrate solution; dissolve trimesic acid (H3BTC) in methanol to obtain a trimesic acid solution; then mix the two solutions to form a mixed solution, and then add the NAS-CDs prepared in step S1, stir under light-proof conditions, and finally separate the product by centrifugation, wash with methanol and water in turn, and then dry to obtain a white precipitate, which is denoted as NAS-CDs / Eu-MOFs. Dissolve the NAS-CDs / Eu-MOFs in PBS buffer solution to obtain a NAS-CDs / Eu-MOFs solution, which is the NAS-CDs / Eu-MOFs fluorescent probe.
[0008] Further, in step S1, the amount ratio of citric acid, ethylenediamine and ultrapure water is 0.2 g ~5 g: 0.5 mL~5 mL: 20 mL~60 mL.
[0009] Further, in step S1, the temperature of the reaction in the high-pressure reaction kettle is 100℃~300℃, and the time is 5 min ~60 min.
[0010] Further, in step S1, the pore size of the water-phase microporous filter membrane is 0.22 μm, and the stirring time under light-proof conditions is 2~2.5 h; the amount ratio of the filtrate, PBS buffer solution and acrydite solution is 10~45 mL: 20~60 mL: 2~10 mL, and the mass concentration of the acrydite solution is 8.5‰.
[0011] Furthermore, in step S2, the ratio of Eu(NO3)3·6H2O to ultrapure water is 10 mg ~ 100 mg : 2 mL ~ 30 mL; the ratio of H3BTC to methanol is 10 mg ~ 150 mg : 2 mL ~ 50 mL. The ratio of europium nitrate hexahydrate to NAS-CDs in the mixed solution is 10 mg ~ 100 mg : 10 mL. When the europium nitrate hexahydrate solution and the trimellitic acid solution are mixed, the ratio of europium nitrate hexahydrate in the europium nitrate hexahydrate solution to trimellitic acid in the trimellitic acid solution is 10 mg ~ 100 mg : 10 mg ~ 150 mg. Furthermore, in step S2, the stirring time in the dark is 0.5 h to 5 h; the drying temperature is 45 to 60 °C and the time is 6 to 12 h; the ratio of NAS-CDs / Eu-MOFs to PBS buffer is 8 to 10 mg: 15 ml.
[0012] This invention also provides the application of the above-mentioned NAS-CDs / Eu-MOFs-based fluorescent probes for acrylamide detection, specifically including the following steps: (1) Construction of the standard curve: Ammonium persulfate (AP) solution and acrylamide standard solution were added to the NAS-CDs / Eu-MOFs solution, and polymerization was carried out under heating. After polymerization for a certain period of time, the reaction was blocked by an ice-water bath. The fluorescence intensity values at 445 nm and 620 nm produced by the fluorescence spectrophotometer at different concentrations of acrylamide were recorded under 365 nm excitation. The fluorescence intensity values were then determined based on the concentration of acrylamide standard and I... 445 / I 620 A standard curve for detecting acrylamide was established by establishing the relationship between the fluorescence intensity ratios; color images of acrylamide standards after polymerization at different concentrations were obtained, and their RGB values were analyzed. Finally, a standard curve was established by establishing the relationship between different concentrations of acrylamide standards and their color R / B ratios. (2) Actual sample testing: Obtain the sample to be tested and replace it with acrylamide standard solution; then further obtain I according to the operation in step (1). 445 / I 620 The fluorescence intensity ratio and color R / B ratio can be substituted into the standard curve to detect acrylamide in the sample.
[0013] Furthermore, in step (1), the concentration of the acrylamide standard solution is 0.01 μmol / L ~ 100 μmol / L, and the volume relationship of the NAS-CDs / Eu-MOFs solution, the acrylamide standard solution and the ammonium persulfate solution is 1.5 mL ~ 2.5 mL: 0.5 mL: 0.05 mL, wherein the concentration of the NAS-CDs / Eu-MOFs solution is 0.2 mg / mL ~ 5 mg / mL, and the mass concentration of the ammonium persulfate solution is 4‰.
[0014] Furthermore, in step (1), the heating temperature of the polymerization reaction is 10℃ ~ 100℃, and the time is 5 min ~ 120 min. Beneficial effects:
[0015] The fluorescent probe constructed in this invention has the advantages of higher sensitivity and faster detection speed compared with traditional techniques such as liquid chromatography and enzyme-linked immunosorbent assay (ELISA) for detecting acrylamide in high-temperature processed foods.
[0016] The NAS-CDs / Eu-MOFs synthesized in this invention have the advantages of thermal stability and simple preparation procedure, which can realize the rapid detection of the target analyte acrylamide.
[0017] Compared with single-fluorescence emission detection of fluorescent materials such as acrylamide, the NAS-CDs / Eu-MOFs dual-emission ratio fluorescent probe constructed in this invention can weaken the interference of external factors, has the advantage of strong anti-interference ability, and can improve the reliability of analysis results.
[0018] Compared with traditional fluorescence detection methods, this invention uses a visualization method that combines colorimetry with smartphones, making it more accessible to the public and thus improving its practical applicability. Attached Figure Description
[0019] Figure 1 This is a diagram showing the optimal reaction temperature for NAS-CDs / Eu-MOFs.
[0020] Figure 2 This is a selectivity diagram of NAS-CDs / Eu-MOFs for acrylamide and its structural analogues.
[0021] Figure 3 These are the fluorescence response curves of NAS-CDs / Eu-MOFs to different concentrations of acrylamide.
[0022] Figure 4 It is an acrylamide standard curve established based on the relationship between acrylamide concentration and fluorescence intensity.
[0023] Figure 5It is an acrylamide standard curve established based on the relationship between acrylamide concentration and color R / B value readings. Detailed Implementation
[0024] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments and accompanying drawings. It should be understood that the specific embodiments described herein are for illustrative purposes only and do not constitute the sole limitation on the scope of protection of this invention.
[0025] In this invention, unless otherwise stated, any numerical range disclosed should be understood to include every intermediate value between the upper and lower limits of that range, and all subranges formed by any combination of these intermediate values. All technical and scientific terms used herein have the meanings commonly understood by one of ordinary skill in the art to which this invention pertains.
[0026] Although preferred embodiments and materials are described herein, those skilled in the art can make obvious modifications, equivalent substitutions, or improvements to the described methods, steps, materials, or parameters without departing from the spirit and essence of the invention, and all such modifications and substitutions should be included within the scope of protection of the invention. This specification and embodiments are merely illustrative and should not be construed as an exhaustive enumeration or undue limitation of the scope of protection of the invention. Example 1:
[0027] S1. Preparation of NAS-CDs: 5 g of anhydrous citric acid was mixed with 2.5 mL of ethylenediamine, and then 40 mL of ultrapure water was quickly added. The mixture was transferred to a high-pressure reactor, and the reaction temperature was set to 180 °C for a fixed reaction time of 45 min. After the reaction, the reactor was allowed to cool naturally to room temperature, and then filtered through a 0.22 μm aqueous microporous membrane. The filtrate was diluted with PBS buffer, and then 8.5‰ NAS solution (dimethyl sulfoxide as solvent) was added. The mixture was stirred for 2.5 h in the dark to prepare NAS-CDs. The volume ratio of filtrate, PBS buffer, and N-succinimide acrylate solution was 45 mL: 50 mL: 5 mL.
[0028] S2. Preparation of NAS-CDs / Eu-MOFs probes 92 mg of Eu(NO3)3·6H2O was dissolved in 20 mL of ultrapure water, and 63 mg of H3BTC was dissolved in 40 mL of methanol, yielding europium nitrate hexahydrate and trimesic acid solutions, respectively. After thoroughly mixing the two solutions, 10 mL of NAS-CDs was added, and the resulting mixture was stirred in the dark at room temperature for 3 h. Finally, the products were separated by centrifugation, washed six times with methanol and six times with ultrapure water, and then dried in a vacuum oven at 45 °C for 12 h to obtain NAS-CDs / Eu-MOFs. Example 2:
[0029] The temperature of the polymerization reaction affects the NAS-CDs / Eu-MOFs detection system.
[0030] To determine the optimal thermal polymerization temperature, different thermal polymerization temperatures were selected to optimize the reaction temperature of the system. The specific steps are as follows: 8 mg of NAS-CDs / Eu-MOFs were added to 15 mL of PBS buffer and stirred thoroughly for 45 min to mix them evenly, thus obtaining the NAS-CDs / Eu-MOFs solution, which is the NAS-CDs / Eu-MOFs fluorescent probe.
[0031] Add 2.45 mL of the prepared NAS-CDs / Eu-MOFs solution, 500 µL of acrylamide standard solution (0.5 μmol / L), and 50 µL of ammonium persulfate solution (4‰) to sterile centrifuge tubes, respectively. Mix thoroughly using a vortex mixer and then place in water baths at temperatures of 30℃, 40℃, 50℃, 60℃, 70℃, and 80℃ for thermal polymerization. When the reaction time reaches 50 min, immediately insert an ice-water bath to stop the reaction. Using a fluorescence spectrophotometer under 365 nm excitation, record the fluorescence intensity values at 445 nm and 620 nm for different concentrations of acrylamide.
[0032] like Figure 1 As shown, as the temperature increases from 30℃ to 80℃, the fluorescence intensity I at 620 nm of the NAS-CDs / Eu-MOFs detection system decreases. 620 The fluorescence intensity I at 445 nm does not change with temperature. 445 The effect gradually increases with increasing temperature, reaching its maximum at a reaction temperature of 60℃. Further increases in temperature result in... 445 The temperature then showed a downward trend. Therefore, 60℃ was chosen as the optimal reaction temperature. Example 3:
[0033] In this embodiment, a substance with a similar structure to acrylamide was selected to evaluate the specificity of the method.
[0034] First, it includes substances with similar structures to acrylamide, such as 6-aminohexanoic acid, butyric acid, propionic acid, and acetic acid, as well as acrylamide precursors such as glycine, L-aspartic acid, and sucrose.
[0035] The specific procedure for selective testing is as follows: A standard solution of 5 μmol / L acrylamide and its structural analogues is accurately prepared. 500 µL of the standard solution, 2.45 mL of the NAS-CDs / Eu-MOFs solution prepared in Example 2, and 50 µL of ammonium persulfate solution are mixed evenly. After thermal polymerization in a water bath at 60 °C for 50 min, the reaction is immediately blocked by an ice-water bath. Subsequently, fluorescence is measured using a fluorescence spectrophotometer. Under excitation at 365 nm, the fluorescence intensity values at 445 nm and 620 nm are recorded, respectively.
[0036] The results are as follows Figure 2 As shown, the ratio I of the fluorescence intensity at 445 nm and 620 nm of the NAS-CDs / Eu-MOFs detection system after the addition of interfering substances is... 445 / I 620 The fluorescence intensity values were all lower than those when acrylamide was added, indicating that the detection method has high selectivity for acrylamide. Example 4:
[0037] In this embodiment, a standard curve for detecting acrylamide was established based on the relationship between the concentration of acrylamide standard and the reading of a fluorescence spectrophotometer, and it was used to detect acrylamide in high-temperature processed foods.
[0038] Establishment of the fluorescence standard curve: Acrylamide standard solutions with concentrations of 0.05 μmol / L, 5 μmol / L, 20 μmol / L, 30 μmol / L, 40 μmol / L, and 50 μmol / L were prepared. 2.45 mL of the NAS-CDs / Eu-MOFs solution prepared in Example 2, 500 µL of the acrylamide standard solution, and 50 µL of ammonium persulfate solution with a mass concentration of 4‰ were added to sterile centrifuge tubes, respectively. The mixtures were vortexed and then placed in a 60°C water bath for thermal polymerization. When the reaction time reached 50 min, the reaction was immediately stopped by placing the tubes in an ice-water bath. Subsequently, using a fluorescence spectrophotometer under 365 nm excitation, the fluorescence intensity values at 445 nm and 620 nm for different concentrations of acrylamide were recorded. The results were then analyzed based on the acrylamide standard concentration and I... 445 / I 620 A standard curve for the detection of acrylamide was established by establishing the relationship between the ratio of fluorescence intensity.
[0039] Figure 3 The fluorescence spectra of NAS-CDs / Eu-MOFs as acrylamide concentration change show that the fluorescence intensity of NAS-CDs / Eu-MOFs at 445 nm gradually increases with the change of acrylamide concentration, while the fluorescence intensity at 620 nm remains unchanged. Figure 4 This figure shows a standard curve for detecting acrylamide in a real sample, constructed using the relationship between fluorescence spectrophotometer readings and acrylamide concentration. The equation of the standard curve is: y = 0.927x + 0.018 (R² - R²) 2 =0.997). The visible fluorescence intensity ratio I... 445 / I 620 It has a good correlation with acrylamide content and can accurately detect acrylamide content. Example 5:
[0040] Establishing the R / B standard curve: Acrylamide standard solutions with concentrations of 0.05 μmol / L, 5 μmol / L, 20 μmol / L, 30 μmol / L, 40 μmol / L, and 50 μmol / L were prepared. 2.45 mL of the NAS-CDs / Eu-MOFs solution prepared in Example 2, 500 µL of the acrylamide standard solution, and 50 µL of the AP solution were added to sterile centrifuge tubes, respectively. The mixtures were vortexed until homogeneous and then placed in a 60°C water bath for thermal polymerization. When the reaction time reached 50 min, the reaction was immediately stopped using an ice-water bath. The colors of the acrylamide standards at different concentrations after the reaction were photographed using the built-in camera of an Apple mobile phone. The RGB values were analyzed using a colorimeter, and a standard curve was established to establish the relationship between the acrylamide standards at different concentrations and their color R / B ratios.
[0041] Figure 5 A standard curve for detecting acrylamide in a real sample was constructed using the R / B value of the reaction solution color and the acrylamide concentration. As shown in the figure, the equation of the standard curve is: y = 0.0142x + 0.424(R... 2 =0.994). It can be seen that there is a good linear relationship between the color R / B value of the reaction solution and the acrylamide content, which can accurately detect the acrylamide content. Example 6:
[0042] Detection of acrylamide in high-temperature processed foods: (1) The toast sample (5 g) was defatted with 10 mL of n-hexane, and the operation was repeated three times. Then, 15 mL of ultrapure water and 15 mL of acetonitrile were mixed evenly and added to the mixed solution. The mixture was then sonicated for 25 min. After that, the mixed solution was centrifuged at 10,000 rpm for 15 min to separate the acrylamide in the sample into the acetonitrile phase. The upper and lower layers were separated using a separatory funnel. QuEChERS buffer salt was added to the lower layer and the mixture was sonicated for 15 min. The purified solution was then centrifuged at 10,000 rpm for 15 min. Finally, the collected supernatant was diluted to 15 mL to obtain the toast crust sample treatment solution. The acrylamide in the obtained toast crust sample treatment solution was determined by high performance liquid chromatography.
[0043] (2) Following the method in Example 3, except that the standard solution was replaced with a toast sample, 500 µL of the toast sample was added to the NAS-CDs / Eu-MOFs solution prepared in Example 2. The fluorescence spectrophotometer reading of acrylamide in the high-temperature processed food was detected and substituted into the obtained standard curve to calculate that the acrylamide content in the toast crust sample was 25.1 μmol / L, thus achieving quantitative detection of acrylamide in high-temperature processed foods. The R / B value of the final reaction solution of the toast sample was analyzed using a color picker installed on a smartphone and substituted into the obtained standard curve to calculate that the acrylamide content in the high-temperature processed food was 25.7 μmol / L, thus achieving quantitative detection of acrylamide in high-temperature processed foods. The results were then compared with those obtained by high-performance liquid chromatography.
[0044] Table 1. Comparison of Fluorescence Ratio Method, Smartphone R / B Value Detection Method, and High Performance Liquid Chromatography
[0045] This table compares the fluorescence ratio method established in this invention with the high-performance liquid chromatography (HPLC) method. It shows that the fluorescent probe constructed based on NAS-CDs / Eu-MOFs in this invention has the advantages of high sensitivity and convenience, enabling quantitative detection of acrylamide content in high-temperature processed food samples. The RSD value of this detection method is 6.1%, indicating good accuracy. Combining it with a color picker installed in a smartphone to analyze the R / B value of the color after the reaction can also achieve quantitative detection of acrylamide content in the sample.
[0046] The embodiments described above are preferred embodiments of the present invention, but the present invention is not limited to the above content. Equivalent substitutions or modifications made by those skilled in the art without departing from the essential spirit of the present invention should fall within the protection scope of the present invention.
Claims
1. A method for constructing a high-sensitivity fluorescent probe for rapid analysis of acrylamide in high-temperature processed foods, characterized by, The method comprises the following steps: S1: Preparation of NAS-CDs: Citric acid and ethylenediamine are mixed, and ultrapure water is added. The obtained mixed solution is transferred to a high-pressure reaction kettle for reaction. After the reaction is completed, the reaction kettle is naturally cooled to room temperature. Then, the obtained filtrate is diluted by adding PBS buffer solution and adding an acrylic acid-N-succinimidyl ester solution. The mixture is stirred in the dark to obtain NAS-CDs. S2: Preparation of NAS-CDs / Eu-MOFs probe: Eu(NO3)3·6H2O is dissolved in ultrapure water to obtain a Eu(NO3)3·6H2O solution. Trimesic acid is dissolved in methanol to obtain a trimesic acid solution. Then, the two solutions are mixed to form a mixed solution, and the NAS-CDs prepared in step S1 are added. The mixture is stirred in the dark. Finally, the product is separated by centrifugation, washed with methanol and water in sequence, and then dried to obtain a white precipitate, which is denoted as NAS-CDs / Eu-MOFs. The NAS-CDs / Eu-MOFs are dissolved in PBS buffer solution to obtain a NAS-CDs / Eu-MOFs solution, which is the NAS-CDs / Eu-MOFs fluorescent probe.
2. The method of claim 1, wherein, In step S1, the amount ratio of citric acid, ethylenediamine and ultrapure water is 0.2 g ~5 g:0.5 mL~5 mL:20 mL~60 mL.
3. The method of claim 1, wherein, In step S1, the temperature of the reaction in the high-pressure reaction kettle is 100℃~300℃, and the reaction time is 5 min ~60 min.
4. The method of claim 1, wherein, In step S1, the pore size of the water-phase microporous filter membrane is 0.22 μm, and the stirring time in the dark is 2~2.5 h. The amount ratio of the filtrate, PBS buffer solution and acrylic acid-N-succinimidyl ester solution is 10~45 mL:20~60 mL:2~10 mL, wherein the mass concentration of the acrylic acid-N-succinimidyl ester solution is 8.5‰.
5. The method of claim 1, wherein, In step S2, the amount ratio of Eu(NO3)3·6H2O and ultrapure water is 10 mg ~ 100 mg:2 mL ~30 mL, and the amount ratio of trimesic acid and methanol is 10 mg ~150mg:2 mL ~50mL.
6. The method of claim 1, wherein, In step S2, when the Eu(NO3)3·6H2O solution and the trimesic acid solution are mixed, the amount ratio of Eu(NO3)3·6H2O in the Eu(NO3)3·6H2O solution to trimesic acid in the trimesic acid solution is 10 mg ~ 100 mg:10 mg ~150 mg. The amount ratio of Eu(NO3)3·6H2O in the mixed solution to NAS-CDs is 10 mg ~ 100 mg:10 mL.
7. The method of claim 1, wherein, In step S2, the stirring time in the dark is 0.5 h~5 h. The drying temperature is 45~60℃, and the drying time is 6~12h. The amount ratio of NAS-CDs / Eu-MOFs to PBS buffer solution is 8~10 mg:15ml.
8. Use of the NAS-CDs / Eu-MOFs fluorescent probe prepared according to any one of claims 1-7 for detection of acrylamide, characterized in that, The method comprises the following steps: (1) Construction of standard curve: In the NAS-CDs / Eu-MOFs solution, add ammonium persulfate solution and acrylamide standard solution, heat polymerization, after a certain time, use ice water bath to stop the reaction, use fluorescence spectrophotometer to record the fluorescence intensity values I of 445 nm and 620 nm produced by fluorescence spectrophotometer to different concentrations of acrylamide under the excitation of 365 nm 445 and I 620 , according to the relationship between the concentration of acrylamide standard and the fluorescence intensity ratio of I 445 / I 620 , a standard curve for detecting acrylamide is established; and obtain the color image after polymerization of acrylamide standard of different concentrations, analyze the RGB value, and finally establish a standard curve between acrylamide standard of different concentrations and the color R / B ratio. (2) Actual sample detection: Obtain the sample to be detected, replace the acrylamide standard solution, and then further obtain I 445 / I 620 The fluorescence intensity ratio, color R / B ratio of the sample to be detected are substituted into the standard curve to realize the detection of acrylamide in the sample to be detected.
9. Use according to claim 8, characterized in that, In step (1), the concentration of the acrylamide standard solution is 0.01 μmol / L ~100 μmol / L, and the amount of the NAS-CDs / Eu-MOFs solution, the ammonium persulfate solution and the acrylamide standard solution is in the ratio of 1.5 mL ~2.5 mL:0.5 mL:0.05 mL, wherein the concentration of the NAS-CDs / Eu-MOFs solution is 0.2 mg / mL~5 mg / mL, and the mass concentration of the ammonium persulfate solution is 4 ‰.
10. Use according to claim 8, characterized in that, In step (1), the temperature of the heating polymerization reaction is 10℃~ 100℃, and the time is 5 min ~ 120min.