Skin anti-aging composition and application thereof

Abstract

The invention provides a skin anti-aging composition and application thereof, and belongs to the technical field of cosmetics. The composition comprises an effective amount of at least two algae extracts selected from spirulina, porphyridium, euglena, phaeodactylum tricornutum and brown algae, and optionally comprises other synergistic anti-aging active ingredients. The skin anti-aging composition prepared by the invention systematically acts on five key dimensions of cell nucleus, mitochondria, extracellular matrix, cell surface communication and cell life cycle, and takes regulation and control of self functions of cells as a core, so that a natural active ingredient composition for actively repairing and reversing age feeling and application thereof are realized.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a skin anti-aging composition and its application. Background Technology

[0002] Skin aging is a complex biological process driven by multiple factors both inside and outside the cell. The concept of anti-aging skincare has evolved from early "defensive" strategies such as anti-oxidation, anti-photoaging, and anti-glycation to the current era of "Cellular Anti-Aging 4.0." The core of this new era lies in moving from addressing macroscopic symptoms to actively regulating microscopic cellular function, aiming to achieve a leap from "delaying aging" to "active repair" and even "reversing the appearance of age" by intervening in the root mechanisms of cellular aging.

[0003] Cellular senescence involves dysregulation at multiple levels, including but not limited to: At the nuclear level: telomere shortening, DNA damage accumulation, and decreased repair capacity determine the cell's replication limit and genome stability.

[0004] At the mitochondrial level: energy metabolism disorders, excessive production of reactive oxygen species, and imbalances in mitochondrial quality control are drivers of cellular aging.

[0005] At the extracellular matrix (ECM) level: Decreased fibroblast activity and excessive activity of matrix metalloproteinases lead to an imbalance in ECM synthesis and degradation, which directly manifests as wrinkles and sagging.

[0006] At the cell surface and communication level: weakened growth factor signaling response and abnormal chronic low-grade inflammation (Inflamm-Aging) signaling disrupt cell homeostasis and repair capabilities.

[0007] At the cellular life cycle level: The accumulation of senescent cells (“zombie cells”) and their harmful senescence-associated secretory phenotype (SASP) pollute the microenvironment, accelerating overall tissue aging.

[0008] Existing anti-aging products mostly focus on a single or a few dimensions (such as simply supplementing collagen or providing basic antioxidants), lacking a systematic solution for the overall "intelligent upgrade" of the cellular factory. Therefore, there is an urgent need to develop a natural and highly effective anti-aging composition that can integrate the above five dimensions, synergistically regulate cellular function, and meet the needs of the Anti-Aging 4.0 era. Summary of the Invention

[0009] The purpose of this invention is to propose a skin anti-aging composition and its application, which systematically acts on five key dimensions of cell nucleus, mitochondria, extracellular matrix, cell surface communication and cell life cycle, with the core of regulating cell self-function, to achieve active repair and reversal of aging. This is a natural active ingredient composition and its application.

[0010] The technical solution of this invention is implemented as follows: The present invention provides a skin anti-aging composition comprising an effective amount of at least two algal extracts selected from Spirulina, Porphyra yezoensis, Euglena, Phaeodactylum tricornutum and brown algae, and optionally containing other synergistic anti-aging active ingredients.

[0011] The composition, through the synergistic effect of its active ingredients, can simultaneously act on the following five dimensions related to cellular aging: (A) Nucleus dimension: Protecting and stabilizing genetic material; (B) Mitochondrial dimension: Improving cellular energy metabolism and quality control; (C) Extracellular matrix dimension: promoting ECM homeostasis and structural remodeling; (D) Cell surface and communication dimensions: regulating cell signaling and inflammatory responses; (E) Cell life cycle dimension: The harmful effects of intervening in senescent cells.

[0012] The following are the specific mechanisms of action of this composition in five dimensions: First Dimension: Cell Nucleus – Guardian of Genes and Blueprints Key targets: telomere length maintenance and DNA damage repair capabilities.

[0013] Core components and mechanisms: Activating Telomerase Activity / Stabilizing Telomeres: The brown algae extract used in this invention (mainly derived from brown algae and *Phaeodactylum tricornutum*) has been found to potentially activate the expression of telomerase reverse transcriptase (TERT) through its unique bioactivity, or indirectly reduce oxidative stress and inflammation-induced damage to telomeres through its powerful antioxidant and anti-inflammatory properties, thereby helping to slow down the rate of telomere shortening. Simultaneously, small-molecule algal peptides extracted from algal proteins such as *Spirulina* and *Plasmodium purpureus* through enzymatic hydrolysis may act as cell messengers, penetrating the skin barrier and upregulating the expression of genes related to telomere maintenance in skin cells.

[0014] Enhancing DNA Repair: Algal polyphenols (such as specific phenolic substances derived from brown algae and *Phaeodactylum tricornutum*) have the potential to activate Sirtuins family proteins (such as SIRT6), a key regulator of DNA damage repair. Components such as β-1,3-glucan (Euglena polysaccharide) from *Euglena* can indirectly increase intracellular NAD+ levels by activating the AMPK pathway, providing sufficient energy for NAD+-dependent repair enzymes such as SIRT6, and enhancing the cell's genome maintenance capacity.

[0015] The second dimension: Mitochondria—the cell's energy engine Key targets: mitochondrial biosynthesis, mitophagy, and mitochondrial oxidative stress.

[0016] Core components and mechanisms: Promoting mitochondrial biogenesis: The phycocyanin and specific algal bioactive peptides in Spirulina extract may activate pathways such as PGC-1α, transducing signals to the cell nucleus and promoting the expression of genes encoding mitochondrial proteins, thereby stimulating the generation of new, healthy mitochondria.

[0017] Inducing mitophagy: The active ingredients contained in the composition of the present invention may promote the selective clearance of dysfunctional senescent mitochondria by regulating mitochondrial membrane potential or related autophagy signaling pathways (such as the PINK1 / Parkin pathway), thereby making room for new mitochondria and optimizing the quality of the mitochondrial network.

[0018] Targeted mitochondrial antioxidant activity: *Phaeodactylum tricornutum* is one of the rich sources of fucoxanthin. Fucoxanthin has a unique mitochondrial-targeted transport mechanism, which can penetrate deep into the mitochondrial matrix, efficiently scavenge free radicals generated within the mitochondria, provide direct and efficient antioxidant protection, and maintain the stability of the mitochondrial electron transport chain.

[0019] The third dimension: the extracellular matrix—the cell's "home" and "support network". Core targets: fibroblast activity and matrix metalloproteinase activity.

[0020] Core components and mechanisms: Empowering fibroblasts: The algal signal peptides contained in this invention (such as those obtained by enzymatic hydrolysis from Spirulina and Chlorella proteins) can mimic endogenous growth factor signals, bind to receptors on the surface of fibroblasts, activate the signaling pathways for the synthesis of collagen, elastin and hyaluronic acid, enhance their synthetic activity, and provide "production instructions".

[0021] Providing raw materials and an optimal environment for synthesis: Spirulina and Euglena are rich in a comprehensive range of amino acids, trace elements, and vitamins (such as vitamin C precursors), providing direct raw materials for the synthesis of the extracellular matrix (ECM). At the same time, macromolecular polysaccharides such as fucoidan and Euglena polysaccharides can form a good hydration network on the skin surface and in the cellular microenvironment, providing an ideal moisture environment for fibroblast activity and ECM assembly.

[0022] Inhibiting ECM degradation: Fucoxanthin in *Phaeodactylum tricornutum* is a potent natural matrix metalloproteinase (MMP) inhibitor that can significantly inhibit the activity of collagenase, elastase, etc., and reduce the excessive breakdown of ECM.

[0023] Fourth Dimension: Cell Surface and Communication – Information Reception and Transmission Key targets: inflammatory signaling pathways and growth factor receptor signaling.

[0024] Core components and mechanisms: Inhibiting chronic inflammation (NF-κB pathway): Fucoxanthin (Phaeodactylum tricornutum) and algal polyphenols are potent inhibitors of the NF-κB pathway. They can prevent the nuclear translocation of the pro-inflammatory transcription factor NF-κB, thereby downregulating the expression of various pro-inflammatory cytokines and chemokines, effectively combating "inflammatory senescence".

[0025] Intelligent immune regulation and signal mimicry: Algal polysaccharides such as fucoidan and Euglena polysaccharides possess "intelligent" immunomodulatory properties, capable of both suppressing excessive inflammatory responses and supporting immune defense when necessary. They can also mimic or optimize the extracellular environment, indirectly influencing signal reception by cell surface receptors.

[0026] Fifth Dimension: Cell Life Cycle – Eliminating “Zombie Cells” Core target: Senescent cells and their SASPs.

[0027] Core components and mechanisms: Inhibition of SASP secretion: Fucoxanthin, with its strong NF-κB inhibitory ability, can effectively inhibit the secretion of harmful SASP in senescent cells, reduce its "contamination" of surrounding healthy cells and its aging-promoting effects, which is in line with the "aging phenotype regulator" strategy.

[0028] Regulating the aging microenvironment: Algal polysaccharides such as fucoidan and Euglena polysaccharide do not directly eliminate cells, but as "microenvironment guardians," they systematically improve the tissue microenvironment in which cells exist through their anti-inflammatory, antioxidant, and moisturizing properties, reduce the overall negative impact of aging cell populations, and may create more favorable conditions for the survival and function of healthy cells.

[0029] As a further improvement of the present invention, the preparation method of the algae extract is as follows: S1. Add algal powder to water, repeatedly freeze and thaw, filter, retain the solid, freeze-dry the filtrate to obtain phycobiliprotein extract; the freeze-thaw method can retain the tetrapyrrole structure of phycobiliprotein to the greatest extent and avoid chromophore degradation and protein denaturation caused by high temperature; S2. Add the solid from step S1 to water, heat to boiling to extract, filter, add ethanol to the filtrate to precipitate, filter, wash, and dry to obtain algal polysaccharide extract; after freeze-thaw extraction of phycobiliprotein, the solid residue can be used for polysaccharide extraction to avoid resource waste. S3. Add the algal polysaccharide extract to N,N-dimethylformamide, add salicylic acid and p-toluenesulfonic acid, heat under reflux and stir to react, remove the solvent under reduced pressure, add the product to ethanol, filter, wash and dry to obtain the algal polysaccharide / salicylic acid complex; using p-toluenesulfonic acid as a catalyst, selective esterification of the hydroxyl groups of algal polysaccharide and the carboxyl groups of salicylic acid is achieved, introducing phenolic hydroxyl groups and benzene ring structures, which significantly improves antioxidant properties; salicylic acid is covalently bound to the polysaccharide backbone through ester bonds, avoiding direct contact of free acid with the skin, making it suitable for sensitive skin.

[0030] S4. Mix the algal polysaccharide / salicylic acid complex and phycobiliprotein extract evenly to obtain the algal extract.

[0031] As a further improvement of the present invention, the repeated freeze-thaw method in step S1 involves freezing the mixture at -20 to -30°C, thawing it at room temperature, and repeating the operation 2-3 times.

[0032] As a further improvement of the present invention, the mass ratio of algae powder to water in step S1 is 1:10-20.

[0033] As a further improvement of the present invention, the mass ratio of the solid to water in step S2 is 1:5-10.

[0034] As a further improvement of the present invention, the mass ratio of the algal polysaccharide extract, salicylic acid and p-toluenesulfonic acid in step S3 is 10:2-3:0.05-0.1.

[0035] As a further improvement of the present invention, the mass ratio of the algal polysaccharide / salicylic acid complex and the phycobiliprotein extract in step S5 is 10:2-3.

[0036] As a further improvement of the present invention, the active ingredients include thickeners, squalane, shea butter, emulsifiers, preservatives, penetration enhancers, ceramides, pH adjusters, and glycerin.

[0037] This invention further protects the use of the above-mentioned skin anti-aging composition in the preparation of cosmetics with antioxidant, whitening, anti-wrinkle, and anti-aging properties.

[0038] The present invention has the following beneficial effects: This invention constructs a three-dimensional, interconnected cellular anti-aging network through five dimensions. For example, protecting the cell nucleus and mitochondria ensures the "fundamental" health and vitality of the cell; healthy cells can more effectively synthesize ECM and respond to normal signals; simultaneously, inhibiting SASP and improving the microenvironment relieves the inhibitory effect on aging cells, creating conditions for the rejuvenation of the entire cell community. The pleiotropic properties of the selected algal components make this network tightly connected and exhibit significant synergistic effects. Detailed Implementation

[0039] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] Preparation Example 1: Algal Extract The preparation method is as follows: S1. Add 10g of algal powder to 100mL of water, freeze the mixture at -20℃, thaw at room temperature, repeat the operation twice, filter, keep the solid, freeze-dry the filtrate to obtain phycobiliprotein extract; The algae powder includes Euglena, Porphyra yezoensis, and Spirulina, in a mass ratio of 2:0.5:1.5; S2. Add 10g of the solid from step S1 to 50mL of water, heat to boiling and extract for 4h, filter, add ethanol to the filtrate until the ethanol content of the system is 80wt%, precipitate for 1h, filter, wash, dry, and obtain algae polysaccharide extract. S3. Add 1g of algal polysaccharide extract to 50mL of N,N-dimethylformamide, add 0.2g of salicylic acid and 0.005g of p-toluenesulfonic acid, heat under reflux and stir for 5h, remove the solvent under reduced pressure, add the product to ethanol, filter, wash and dry to obtain algal polysaccharide / salicylic acid complex. S4. Mix 1g of algal polysaccharide complex and 0.2g of phycobiliprotein extract evenly to obtain algal extract.

[0041] Preparation Example 2: Algal Extract The preparation method is as follows: S1. Add 10g of algal powder to 200mL of water, freeze the mixture at -30℃, thaw at room temperature, repeat the operation 3 times, filter, keep the solid, freeze-dry the filtrate to obtain phycobiliprotein extract; The algae powder includes Euglena, Porphyra yezoensis, and Spirulina, in a mass ratio of 2:0.5:1.5; S2. Add 10g of the solid from step S1 to 100mL of water, heat to boiling and extract for 4h, filter, add ethanol to the filtrate until the ethanol content of the system is 80wt%, precipitate for 1h, filter, wash, dry, and obtain algae polysaccharide extract. S3. Add 1g of algal polysaccharide extract to 50mL of N,N-dimethylformamide, add 0.3g of salicylic acid and 0.01g of p-toluenesulfonic acid, heat under reflux and stir for 5h, remove the solvent under reduced pressure, add the product to ethanol, filter, wash and dry to obtain algal polysaccharide / salicylic acid complex. S4. Mix 1g of algal polysaccharide / salicylic acid complex and 0.3g of phycobiliprotein extract evenly to obtain algal extract.

[0042] Preparation Example 3: Algal Extract The preparation method is as follows: S1. Add 10g of algal powder to 150mL of water, freeze the mixture at -25℃, thaw at room temperature, repeat the operation 3 times, filter, keep the solid, freeze-dry the filtrate to obtain phycobiliprotein extract. The algae powder includes Euglena, Porphyra yezoensis, and Spirulina, in a mass ratio of 2:0.5:1.5; S2. Add 10g of the solid from step S1 to 80mL of water, heat to boiling and extract for 4h, filter, add ethanol to the filtrate until the ethanol content of the system is 80wt%, precipitate for 1h, filter, wash, dry, and obtain algae polysaccharide extract. S3. Add 1g of algal polysaccharide extract to 50mL of N,N-dimethylformamide, add 0.25g of salicylic acid and 0.007g of p-toluenesulfonic acid, heat under reflux and stir for 5h, remove the solvent under reduced pressure, add the product to ethanol, filter, wash and dry to obtain algal polysaccharide / salicylic acid complex. S4. Mix 1g of algal polysaccharide / salicylic acid complex and 0.25g of phycobiliprotein extract evenly to obtain algal extract.

[0043] Comparative preparation example 1: The difference from preparation example 3 is that step S3 was not performed.

[0044] The preparation method is as follows: S1. Add 10g of algal powder to 150mL of water, freeze the mixture at -25℃, thaw at room temperature, repeat the operation 3 times, filter, keep the solid, freeze-dry the filtrate to obtain phycobiliprotein extract. The algae powder includes Euglena, Porphyra yezoensis, and Spirulina, in a mass ratio of 2:0.5:1.5; S2. Add 10g of the solid from step S1 to 80mL of water, heat to boiling and extract for 4h, filter, add ethanol to the filtrate until the ethanol content of the system is 80wt%, precipitate for 1h, filter, wash, dry, and obtain algae polysaccharide extract. S3. Mix 1g of algal polysaccharide extract and 0.25g of phycobiliprotein extract evenly to obtain algal extract.

[0045] Comparative preparation example 2: The difference compared to Preparation Example 3 is that no phycobiliprotein extract was added.

[0046] The preparation method is as follows: S1. Add 10g of algae powder to 150mL of water, freeze the mixture at -25℃, thaw at room temperature, repeat the operation 3 times, filter, and keep the solid for later use; the algae powder includes Euglena, Porphyra yezoensis, and Spirulina in a mass ratio of 2:0.5:1.5; S2. Add 10g of the solid from step S1 to 80mL of water, heat to boiling and extract for 4h, filter, add ethanol to the filtrate until the ethanol content of the system is 80wt%, precipitate for 1h, filter, wash, dry, and obtain algae polysaccharide extract. S3. Add 1g of algal polysaccharide extract to 50mL of N,N-dimethylformamide, add 0.25g of salicylic acid and 0.007g of p-toluenesulfonic acid, heat under reflux and stir for 5h, remove the solvent under reduced pressure, add the product to ethanol, filter, wash and dry to obtain the algal polysaccharide / salicylic acid complex, which is the algal extract.

[0047] Comparative preparation example 3: The difference compared to Preparation Example 3 is that no algal polysaccharide / salicylic acid complex was added.

[0048] The preparation method is as follows: S1. Add 10g of algae powder to 150mL of water, freeze the mixture at -25℃, thaw at room temperature, repeat the operation 3 times, filter, keep the solid, freeze-dry the filtrate to obtain phycobiliprotein extract, which is algae extract.

[0049] Test Example 1: Antioxidant Efficacy Test The algal extracts prepared in Preparation Examples 1-3 and Comparative Preparation Examples 1-3 were determined by their DPPH free radical scavenging ability.

[0050] Test solution: 1 mg / mL solution of algal extract prepared in Preparation Examples 1-3 and Comparative Preparation Examples 1-3 using deionized water; Positive control: Control VC.

[0051] Accurately weigh 0.02604 g of DPPH (96% purity), dissolve it in 95% ethanol and bring the volume to 100 mL. The DPPH concentration is 0.25 g·L⁻¹. -1 Store for later use. For experiments, take 12 mL of 0.25 g·L⁻¹. -1 The DPPH solution was diluted to 100 mL with 95% ethanol, resulting in a DPPH concentration of 0.03 g·L⁻¹. -1 Using 95% ethanol as a reference solution, the baseline was scanned. The result was 0.03 g·L⁻¹. -1 The DPPH solution showed a maximum absorption wavelength of 518 nm when scanned at 400-600 nm.

[0052] Determination of absorbance of blank control: Take 5 mL of 0.03 g·L⁻¹ DPPH solution into a 10 mL EP tube, then add 0.1 mL of 95% ethanol solution and mix. Measure the absorbance at 518 nm and record it as A0.

[0053] Determination of absorbance of the test solution: Take 5 mL of 0.03 g·L⁻¹ solution. -1 Pour the DPPH solution into an EP tube, then add 0.1 mL of the test solution and mix well. Measure the absorbance at 518 nm and record it as A. i .

[0054] Data processing: The clearance rate E is calculated from the data obtained above using the following formula: E = 1 - A i / A0×100% The free radical scavenging rate results obtained are shown in Table 1: Table 1. Free radical scavenging rate of solution

[0055] As can be seen from the table above, the algal extracts prepared in Examples 1-3 of the present invention have good antioxidant effects.

[0056] Example 1: A Cellular Five-Dimensional Anti-Aging Essence Formulation composition (weight percentage): Fucoidan (derived from brown algae / Phaeodactylum tricornutum) 1.0%; Fucoxanthin (derived from *Phaeodactylum tricornutum*) 0.8%; Preparation Example 1 yielded 4% of the algal extract; Glycerin (humectant) 4.0%; 1,3-Propanediol (moisturizing and penetration enhancer) 3.0%; Xanthan gum (thickener) 0.1%; Phenoxyethanol / ethylhexylglycerin (preservative) 0.8%; Add deionized water to a concentration of 100%.

[0057] Preparation method: (1) Disperse xanthan gum in a portion of deionized water and stir until it is completely swollen.

[0058] (2) Dissolve glycerol, 1,3-propanediol, fucoidan and algae extract in the remaining water and mix well.

[0059] (3) After dispersing the fucoxanthin with a small amount of co-solvent, add it to the aqueous phase of step 2 and stir until homogeneous.

[0060] (4) Add the mixture from steps 2 and 3 to the colloid from step 1 and stir until homogeneous.

[0061] (5) Add preservatives, stir well, adjust pH to 5.5-6.0, and then fill.

[0062] Example 2: A Cellular Five-Dimensional Anti-Aging Essence The difference from Example 1 is that the algae extract was prepared in Preparation Example 2.

[0063] Example 3: A Cellular Five-Dimensional Anti-Aging Essence The difference from Example 1 is that the algae extract was prepared in Preparation Example 3.

[0064] Comparative Examples 1-4: The difference from Example 3 is that the algal extract was prepared from Comparative Preparation Examples 1-4.

[0065] Test Example 2: Human fibroblasts cultured in DMEM medium containing 12% FBS were seeded in 96-well plates. After cell attachment, the medium was exchanged with DMEM medium containing 0.2% FBS and 250 μM ascorbate magnesium phosphate. 0.1 mL of the cell five-dimensional anti-aging essence prepared in Examples 1-3 and Comparative Examples 1-4 was added, mixed well, and cultured for 72 h. The supernatant was collected, centrifuged, and the content of type III collagen in the supernatant was determined.

[0066] The content of procollagen type III-peptide (PⅢP) in the supernatant was determined using the RIA-gnost PⅢP (PⅢP) assay kit. The anti-wrinkle effect of these samples was then evaluated by the rate of promotion of type III collagen synthesis. The calculation formula is as follows: Promotion rate = (W1-W0) / W0*100% W1: Type III collagen production after adding the test sample; W0: Type III collagen production without the addition of test samples.

[0067] The results are shown in Table 2.

[0068] Table 2

[0069] As shown in the table above, the cell five-dimensional anti-aging essence prepared in Examples 1-4 of the present invention has a significant effect on promoting type III collagen, thickening the extracellular matrix, and thus achieving an anti-aging effect.

[0070] Example 4: A Cellular Five-Dimensional Anti-Aging Repair Cream Formulation composition (weight percentage): Preparation Example 3 yielded an algal extract of 9.0%; Ceramide NP (strengthens the skin barrier) 0.5%; Squalane (similar to sebum) 5.0%; Shea butter (moisturizing) 3.0%; Cetearyl alcohol olive oil ester (emulsifier) ​​3.5%; Glycerin 5.0%; Carbomer (thickener) 0.2%; Triethanolamine (pH adjuster), appropriate amount; Add deionized water to a concentration of 100%.

[0071] Preparation method: The conventional oil phase and aqueous phase are heated to 75-80℃ respectively. The oil phase is added to the aqueous phase for homogenization and emulsification. After cooling to below 45℃, algae extract is added, stirred evenly, and the pH is adjusted to 5.5-6 before filling.

[0072] Comparative preparation examples 4-6: The difference from Example 4 is that the algal extract was prepared from Comparative Preparation Examples 1-3.

[0073] Test Example 3: Skin texture was measured using the cell-based five-dimensional anti-aging repair creams prepared in Example 4 and Comparative Examples 4-6 of this invention. First, 50 healthy female subjects were selected. The left side of each subject's face was treated with the cell-based five-dimensional anti-aging repair cream prepared in this invention (experimental group), while the other side of the face remained untreated (blank group). The experiment lasted for 8 weeks. Skin analysis was performed on the subjects' skin using various instruments at weeks 1 and 8 to evaluate the effects of the toner on improving skin moisture content, skin elasticity, fine lines, and skin roughness. The average values ​​from the three tests at weeks 1 and 8 are shown in Table 3.

[0074] Table 3

[0075] As can be seen from the table above, the cell five-dimensional anti-aging repair cream prepared in Example 4 of the present invention has good effects in removing wrinkles, fading fine lines, and improving skin.

[0076] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A skin anti-aging composition, characterized in that, The composition comprises effective amounts of at least two algal extracts selected from Spirulina, Porphyra yezoensis, Euglena, Phaeodactylum tricornutum and brown algae, and optionally contains other synergistic anti-aging active ingredients.

2. The skin anti-aging composition according to claim 1, characterized in that, The preparation method of the algal extract is as follows: S1. Add algal powder to water, repeatedly freeze and thaw, filter, keep the solid, freeze-dry the filtrate to obtain phycobiliprotein extract; S2. Add the solid from step S1 to water, heat to boiling to extract, filter, add ethanol to the filtrate to precipitate, filter, wash, dry, and obtain algae polysaccharide extract; S3. Extract the polysaccharide from the algae; S4. Mix the algal polysaccharide / salicylic acid complex and phycobiliprotein extract evenly to obtain the algal extract.

3. The skin anti-aging composition according to claim 2, characterized in that, The repeated freeze-thaw method described in step S1 involves freezing the mixture at -20 to -30°C, thawing it at room temperature, and repeating the operation 2-3 times.

4. The skin anti-aging composition according to claim 2, characterized in that, The mass ratio of algae powder to water in step S1 is 1:10-20.

5. The skin anti-aging composition according to claim 2, characterized in that, The mass ratio of the solid to water in step S2 is 1:5-10.

6. The skin anti-aging composition according to claim 2, characterized in that, The mass ratio of the algal polysaccharide extract in step S3 is 10:2-3:0.05-0.

1.

7. The skin anti-aging composition according to claim 2, characterized in that, The mass ratio of the algal polysaccharide / salicylic acid complex and the phycobiliprotein extract in step S5 is 10:2-3.

8. The skin anti-aging composition according to claim 1, characterized in that, The active ingredients include thickeners, squalane, shea butter, emulsifiers, preservatives, penetration enhancers, ceramides, pH adjusters, and glycerin.

9. The use of a skin anti-aging composition as described in any one of claims 1-9 in the preparation of cosmetics with antioxidant, whitening, anti-wrinkle, and anti-aging properties.

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