Method for synergistically regulating autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation

Abstract

The invention discloses a method for cooperatively regulating and controlling autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation. The method comprises the following steps: constructing a composite biological system containing autotrophic anaerobic ammonia oxidation granular sludge and a heterotrophic nitrobenzene degrading bacterium Bacillus pumilus NJUST51 under an anaerobic condition; adding wastewater containing ammonia nitrogen and nitrite nitrogen into the composite biological system; nitrobenzene is added into the composite biological system, and an external organic carbon source is added; by regulating the concentration of an external organic carbon source to be 50-300 mg COD L <-1 > and regulating the mass ratio of anaerobic ammonium oxidation granular sludge to heterotrophic nitrobenzene degrading bacteria Bacillus pumilus NJUST51 to be (1: 3)-(3: 1), the nitrobenzene degradation process and the anaerobic ammonium oxidation denitrification process are synchronously carried out, so that the synergistic enhancement of nitrogen removal and nitrobenzene removal is realized.

Description

Technical Field

[0001] This invention relates to the fields of environmental protection and wastewater treatment, specifically to a synergistic microbial system and application method for nitrobenzene degradation and nitrogen removal in nitrobenzene-containing wastewater, and in particular to a technology based on the co-cultivation of anaerobic ammonia-oxidizing bacteria and anaerobic sludge to synergistically achieve nitrobenzene degradation and denitrification. Background Technology

[0002] With the rapid development of industries such as pharmaceuticals, dyes, pesticides, and fine chemicals, the discharge of high-nitrogen wastewater containing nitro aromatic compounds continues to increase. This type of wastewater typically exhibits a complex characteristic of high ammonia nitrogen, high salinity, and the coexistence of recalcitrant organic pollutants. Among these, nitro aromatic compounds, due to the strong electron-withdrawing conjugation effect between the benzene ring and the nitro group, have a stable structure, strong environmental persistence, and degrade slowly under natural conditions, while also exhibiting significant toxic and inhibitory effects on microorganisms. At the same time, the high-salt environment easily causes osmotic pressure imbalance in microbial cells and reduces the activity of key enzymes, further weakening the stability and treatment efficiency of biological treatment systems.

[0003] Against this backdrop, anammox (anammox) technology is considered an important technological direction for treating high-nitrogen wastewater due to its advantages of not requiring external organic carbon sources, high denitrification efficiency, and low sludge production. However, existing anammox systems are mainly suitable for low organic load conditions. When wastewater contains recalcitrant organic matter such as nitrobenzene, its autotrophic microbial community is easily suppressed, denitrification performance declines, and the granular sludge structure may also become unstable.

[0004] Patent specification CN114703090A discloses a moderately halophilic Bacillus strain capable of degrading nitrobenzene. Molecular biological identification confirms it as… Bacillus Named Bacillus pumilus NJUST51, with accession number CCTCC NO: M2022199, is a moderately halophilic Bacillus that can be cultured and grown in a high-salt environment (8.5%~9.5% w / v NaCl) while simultaneously achieving efficient degradation of nitrobenzene.

[0005] However, the Anammox autotrophic denitrification process and the heterotrophic nitrobenzene degradation process often exhibit inherent contradictions within the same system. The bioreduction of nitrobenzene relies on heterotrophic bacteria utilizing organic carbon as electron donors to complete the stepwise reduction of nitro groups. Anaerobic ammonia oxidizing bacteria, being obligate autotrophic microorganisms, are highly sensitive to changes in organic matter concentration. When the proportion of organic carbon in the system is too high, heterotrophic bacteria easily compete for key electron acceptors such as nitrite, weakening the anaerobic ammonia oxidation reaction rate and disrupting particle structure. Conversely, when the proportion of organic carbon is too low, it is difficult to maintain sufficient reduction of nitrobenzene, and the accumulation of toxic intermediates, in turn, inhibits the activity of autotrophic bacteria.

[0006] Therefore, how to achieve the synchronous and stable operation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation processes under conditions of high salt and coexistence of nitroaromatic compounds, rationally control the ratio of organic carbon to nitrogen load, optimize the quantity ratio and spatial structure of autotrophic and heterotrophic bacteria, avoid electron acceptor competition and metabolic pathway conflicts, and thus construct a metabolically complementary and synergistically enhanced composite microbial system has become a key problem that current technology has not yet effectively solved, and it is also a core technological bottleneck that urgently needs to be overcome in this field. Summary of the Invention

[0007] To address the aforementioned technical problems and shortcomings in the field, this invention provides a method for the synergistic regulation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation. This method can simultaneously enhance the autotrophic anaerobic ammonia oxidation process and the heterotrophic nitrobenzene degradation process, solving the problem in the prior art where the imbalance of organic carbon ratio leads to the inhibition of anaerobic ammonia oxidation or insufficient nitrobenzene degradation. This allows for the construction of a composite biological system in which autotrophic and heterotrophic bacteria have complementary metabolisms and operate stably in a synergistic manner.

[0008] The specific technical solution is as follows: A method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation includes: Constructing a system containing autotrophic anammox granular sludge and heterotrophic nitrobenzene-degrading bacteria under anaerobic conditions Bacillus pumilus NJUST51's composite biological system; Wastewater containing ammonia nitrogen and nitrite nitrogen was added to the complex biological system; Nitrobenzene was added to the complex biological system, along with an external organic carbon source; By adjusting the concentration of the added organic carbon source to 50-300 mg COD L... -1 (e.g., 150 mg COD L) -1 (etc.), and regulate the interaction between anaerobic ammonia oxidation granular sludge and heterotrophic nitrobenzene degrading bacteria. Bacillus pumilus The mass ratio of NJUST51 is (1:3) to (3:1) (e.g., 1:1), which allows the nitrobenzene degradation process to proceed simultaneously with the anaerobic ammonia oxidation denitrification process, thereby achieving synergistic enhancement of nitrogen removal and nitrobenzene removal.

[0009] The heterotrophic nitrobenzene-degrading bacteria described in this invention Bacillus pumilus NJUST51 is prior art; see the patent specification with publication number CN114703090A. This strain is deposited at the China Center for Type Culture Collection, with accession number CCTCC NO: M2022199.

[0010] In this invention, anaerobic ammonia oxidation granular sludge and heterotrophic nitrobenzene-degrading bacteria are used. Bacilluspumilus The quality of NJUST51 can be determined in the following ways: Determine the concentration of volatile suspended solids in anaerobic ammonia oxidation granular sludge; Measurement Bacillus pumilus Concentration of volatile suspended solids in NJUST51 bacterial culture (e.g., OD600 of 1, etc.); When constructing a composite biological system, the mass of the anammox granular sludge is the product of the volume of the added anammox granular sludge and the concentration of volatile suspended solids in the anammox granular sludge. Bacillus pumilus NJUST51 bacterial culture volume and Bacillus pumilus The product of the volatile suspended solids concentrations of NJUST51 bacterial culture is... Bacillus pumilus The quality of NJUST51.

[0011] In some preferred embodiments, the method for synergistic regulation of autotrophic anammox and heterotrophic nitrobenzene degradation, when constructing the composite biological system, involves adding anammox granular sludge with a volatile suspended solids concentration of 12.8–15.6 g / L. -1 .

[0012] In some preferred embodiments, the method for synergistic regulation of autotrophic anammox and heterotrophic nitrobenzene degradation, wherein the anammox granular sludge in the composite biological system and Bacillus pumilus The total volatile suspended solids concentration of NJUST51 was controlled at 1.55 ± 0.72 g / L. -1 .

[0013] In some preferred embodiments, the method for synergistic regulation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation is wherein the molar ratio of ammonia nitrogen to nitrite nitrogen in the wastewater is (0.85~1.15):(0.85~1.15), and more preferably 1:1.

[0014] In some preferred embodiments, in the method for synergistic regulation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation, the mass concentrations of ammonia nitrogen and nitrite nitrogen in the wastewater are selected from 40-60 mg / L, respectively. -1 For example, 50 mg L -1 wait.

[0015] In some preferred embodiments, the method for synergistic regulation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation further includes the addition of inorganic salts, trace element solution I, and trace element solution II to the wastewater to meet the needs of microbial growth.

[0016] Furthermore, in the method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation, the addition of inorganic salts results in the following final concentration composition of the wastewater: NaHCO3 0.84 g / L. -1 NaH2PO4 0.01 g L -1 MgSO4·7H2O 0.0568 g L -1 CaCl2 0.0735 g L -1 .

[0017] In some preferred embodiments, the method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation involves adding trace element solution I to the wastewater at a ratio of 1 mL / L. -1 ; Trace element solution I comprises the following components: EDTA 5.00 g / L -1 FeSO4 9.14 g L -1 ; The solvent for trace element solution I is distilled water.

[0018] In some preferred embodiments, the method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation involves adding trace element solution II to the influent at a ratio of 1 mL / L. -1 ; Trace element solution II comprises the following components: EDTA 15.0 g / L -1 ZnSO4·7H2O 0.430 g L -1 CoCl2·6H2O 0.240 g L -1 MnCl2·4H2O 0.990 g L -1 CuSO4·5H2O 0.250 g L -1 NaMoO4·2H2O 0.220 g L -1 NiCl2·6H2O 0.210 g L -1 H3BO4 0.014 g L -1 ; The solvent for Trace Element Solution II is distilled water.

[0019] In some preferred embodiments, the method for synergistic regulation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation includes glucose as the external organic carbon source. Further, the glucose concentration in the wastewater can be 46.7~280.4 mg / L. -1 (Corresponding to an external organic carbon source concentration of 50~300 mg COD L) -1 For example, 140.2 mg L -1(Corresponding to an external organic carbon source concentration of 150 mg COD L) -1 )wait.

[0020] This invention can monitor NH4 + -N, NO2 - -N, NO3 - The nitrogen conversion pathway and organic matter conversion efficiency were calculated based on the changes in the concentrations of -N, total nitrogen, nitrobenzene, and aniline, and the collaborative operation window was determined.

[0021] This invention achieves synchronized and stable operation of the autotrophic denitrification process and the heterotrophic organic matter reduction process within the system by adjusting the concentration of the added organic carbon source and the ratio of the two types of functional bacteria. In some preferred embodiments, the method for synergistic regulation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation involves adjusting the concentration of the added organic carbon source to 150 mg COD L. -1 Furthermore, it regulates the interaction between anaerobic ammonia oxidation granular sludge and heterotrophic nitrobenzene-degrading bacteria. Bacillus pumilus The NJUST51 mass ratio is 1:1, which can achieve the optimal state of simultaneous nitrogen removal and nitrobenzene removal.

[0022] In some preferred embodiments, the method for synergistic regulation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation is used to operate the composite biological system under conditions of light avoidance and 35±1℃.

[0023] In some preferred embodiments, the method for synergistic regulation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation is used to control the pH of the wastewater at 7.2 to 7.4.

[0024] In some preferred embodiments, the method for synergistic regulation of autotrophic anammox and heterotrophic nitrobenzene degradation employs a batch reaction system.

[0025] In some preferred embodiments, the method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation involves adding an initial concentration of nitrobenzene to the wastewater of no more than 10 mg / L. -1 .

[0026] This invention establishes a rational distribution mechanism of metabolic electron flow by regulating the intensity of the added organic carbon source and the ratio of autotrophic to heterotrophic bacteria, thereby enabling heterotrophic bacteria to... Bacillus pumilus NJUST51 utilizes glucose as an electron donor to complete the stepwise reduction of nitrobenzene, while avoiding nitrite competition and anaerobic ammonia oxidation activity inhibition caused by excessive organic carbon, thus achieving simultaneous enhancement of organic matter degradation and autotrophic denitrification processes.

[0027] Compared with the prior art, the beneficial effects of this invention are as follows: 1) By using a dual regulation strategy of carbon source gradient and microbial community ratio, the optimal window range for autotrophic-heterotrophic synergistic operation was determined.

[0028] 2) While ensuring efficient removal of nitrobenzene, maintain stable operation of anaerobic ammonia oxidation to avoid system imbalance caused by the enhancement of a single process.

[0029] 3) By revealing the interaction between carbon source intensity and microbial community structure through full factorial design, a theoretical basis is provided for the engineering application of high-salt, high-nitrogen, nitrate-containing aromatic wastewater.

[0030] 4) The constructed system operates under mild conditions and is easy to operate, with good potential for scalability. Attached Figure Description

[0031] Figure 1 The figure shows the dynamic response curves of different bacterial community ratios and the concentration of added organic carbon source to denitrification efficiency.

[0032] Figure 2 The dynamic response curves of different bacterial community ratios and added organic carbon source concentrations on the degradation efficiency of nitrobenzene are shown. Detailed Implementation

[0033] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention.

[0034] Example 1: Autotrophic anaerobic ammonia-oxidizing bacteria and heterotrophic bacteria Bacillus pumilus The construction of the NJUST51 collaborative system: In this embodiment, a batch anaerobic reaction system was constructed using 100 mL sealed serum bottles. Before the experiment, the system was purged with high-purity nitrogen for 30 min to ensure an anaerobic environment. After sealing, the system was placed in a constant-temperature shaking incubator. The system was operated at a temperature of 35±1℃, a shaking speed of 150 rpm, and incubated in the dark for a reaction period of 20 h.

[0035] Inoculated sludge includes anaerobic ammonia oxidation granular sludge and anaerobic functional bacteria. Bacillus pumilus NJUST51. Anaerobic ammonia oxidation granular sludge was taken from a stably operating upflow anaerobic sludge blanket reactor, with a volatile suspended solids (VSS) concentration of 12.8–15.6 g / L. -1 The anaerobic ammonia oxidation activity was 15.1~25.3 mg NO2. - -N g -1 VSS h -1 anaerobic bacteria Bacillus pumilusNJUST51 is a nitrobenzene-reducing bacterial community. The final VSS in each serum bottle was controlled at 1.55 ± 0.72 g / L. -1 .

[0036] The simulated wastewater composition includes: NaHCO3 0.84 g / L -1 NaH2PO4 0.01 g L -1 MgSO4·7H2O 0.0568g L -1 CaCl2 0.0735 g L -1 NH4Cl (provides ammonia nitrogen), NaNO2 (provides nitrite nitrogen), with an ammonia nitrogen to nitrite nitrogen molar ratio of 1:1 and a concentration of 50 mg / L for both. -1 Trace element solution I 1 mL·L -1 Trace element solution II 1 mL·L -1 The initial concentration of nitrobenzene was 10 mg / L. -1 The solvent is water, and the pH of the wastewater is adjusted to 7.2-7.4. Trace element solution I comprises the following components: EDTA 5.00 g / L. -1 FeSO4 9.14 g L -1 Trace element solution I is dissolved in distilled water. Trace element solution II consists of the following components: EDTA 15.0 g / L. -1 ZnSO4·7H2O 0.430 g L -1 CoCl2·6H2O 0.240 g L -1 MnCl2·4H2O 0.990 g L -1 CuSO4·5H2O 0.250 g L -1 NaMoO4·2H2O 0.220 g L -1 NiCl2·6H2O 0.210 g L -1 H3BO4 0.014 g L -1 The solvent for Trace Element Solution II is distilled water. The added organic carbon source is glucose, and three concentration gradients (based on COD) are set: 50 mg COD / L. -1 150 mg COD L -1 and 300 mg COD L -1 The corresponding glucose concentrations were 46.7 mg / L. -1 140.2 mg L -1 and 280.4 mg L -1 .

[0037] Example 2: Experiments with different bacterial community ratios and gradient combinations of added organic carbon sources: Based on Example 1, anaerobic ammonia oxidation granular sludge and Bacillus pumilus NJUST51 was available in three mass ratios: 3:1, 1:1, and 1:3, and was mixed with three glucose concentrations (46.7 mg / L). -1 140.2 mg L -1 280.4 mg L -1 We conducted a full factorial combination to construct nine experimental systems, G1 to G9, to analyze the main effects and interactions between carbon source intensity and bacterial community ratio.

[0038] To verify the synergistic mechanism of the system and eliminate interfering factors, the following control group was set up: C1 biodegradation baseline group: anaerobic ammonia oxidation granular sludge and Bacillus pumilus NJUST51 has a mass ratio of 1:1, with no added glucose, only nitrobenzene added; C2 Carbon Source Impact Group: Anaerobic Ammonium Oxidation Granular Sludge and Bacillus pumilus NJUST51 was added at a mass ratio of 1:1, with an addition of 280.4 mg / L. -1 Glucose, without added nitrobenzene; C3 Non-biological Control Group: Inactivated sludge (anaerobic ammonium oxidation granular sludge and...) Bacillus pumilus NJUST51 (mass ratio 1:1), nitrobenzene and 280.4 mg L were added. -1 glucose; C4 methanol blank group; C5 is inoculated only with anaerobic ammonia oxidation sludge; C6 only vaccinated Bacillus pumilus NJUST51.

[0039] Each experimental group ran for 20 hours, and NH4 was measured periodically. + -N, NO2 - -N, NO3 - -N, total nitrogen, nitrobenzene and aniline concentrations were used to calculate the removal rate and conversion pathway.

[0040] See Figure 1 and Figure 2 Experimental results showed that when the glucose concentration was 150 mg COD L... -1 And anaerobic ammonia oxidation sludge and Bacillus pumilus When the mass ratio of NJUST51 is 1:1, the system achieves the optimal state of simultaneous nitrogen removal and nitrobenzene degradation, characterized by NH4+. + -N, NO2- The removal rates of -N and total nitrogen were high, while the removal rate of nitrobenzene was improved and the accumulation rate of aniline was low. When the carbon source concentration was too high or the bacterial community ratio was unbalanced, nitrite competition or insufficient degradation of organic matter occurred, leading to a decrease in the synergistic effect. Therefore, the optimal window range for autotrophic-heterotrophic synergistic operation was determined, providing a basis for constructing a stable coupled and enhanced system.

[0041] In summary, this invention constructs an autotrophic anammox granular sludge and heterotrophic nitrobenzene-degrading bacteria under anaerobic conditions. Bacillus pumilus The NJUST51 composite system achieves simultaneous and stable operation of the nitrobenzene reduction process and the anaerobic ammonia oxidation denitrification process by adjusting the concentration of the added organic carbon source and the ratio of the two types of functional bacteria. The added organic carbon source, taking glucose as an example, is set at 50-300 mg COD / L. -1 The anaerobic ammonia oxidation granular sludge and heterotrophic nitrobenzene-degrading bacteria Bacillus pumilus The mass ratio of NJUST51 is (1:3) to (3:1). Within this control range, it can avoid the competition for nitrite and the inhibition of anaerobic ammonia oxidation activity caused by excessive organic carbon, while ensuring the effective degradation of nitrobenzene, thus achieving synergistic enhancement of nitrogen removal and organic pollutant removal.

[0042] This invention constructs an autotrophic-heterotrophic metabolic complementarity mechanism, providing a technical basis for the efficient and stable treatment of high-nitrogen wastewater containing nitro aromatic compounds.

[0043] Furthermore, it should be understood that after reading the above description of the present invention, those skilled in the art can make various alterations or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims.

Claims

1. A method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation, characterized in that, include: Constructing a system containing autotrophic anammox granular sludge and heterotrophic nitrobenzene-degrading bacteria under anaerobic conditions Bacillus pumilus NJUST51's composite biological system; Wastewater containing ammonia nitrogen and nitrite nitrogen was added to the complex biological system; Nitrobenzene was added to the complex biological system, along with an external organic carbon source; By adjusting the concentration of the added organic carbon source to 50-300 mg COD L... -1 Furthermore, it regulates the interaction between anaerobic ammonia oxidation granular sludge and heterotrophic nitrobenzene-degrading bacteria. Bacillus pumilus The mass ratio of NJUST51 is (1:3)~(3:1), which allows the nitrobenzene degradation process to proceed simultaneously with the anaerobic ammonia oxidation denitrification process, thereby achieving synergistic enhancement of nitrogen removal and nitrobenzene removal.

2. The method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation according to claim 1, characterized in that, The volatile suspended solids concentration of the anaerobic ammonia oxidation granular sludge added during the construction of the composite biological system was 12.8–15.6 g / L. -1 ; Anaerobic ammonia oxidation granular sludge in a composite biological system Bacillus pumilus The total volatile suspended solids concentration of NJUST51 was controlled at 1.55 ± 0.72 g / L. -1 .

3. The method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation according to claim 1, characterized in that, The molar ratio of ammonia nitrogen to nitrite nitrogen in the wastewater is (0.85~1.15):(0.85~1.15), and more specifically 1:1; The mass concentrations of ammonia nitrogen and nitrite nitrogen in the wastewater were selected from 40-60 mg / L, respectively. -1 .

4. The method for synergistic regulation of autotrophic anaerobic ammonia oxidation and heterotrophic nitrobenzene degradation according to claim 1, characterized in that, The wastewater also contains inorganic salts, trace element solution I, and trace element solution II to meet the needs of microbial growth; The addition of inorganic salts resulted in the following final concentration composition of the wastewater: NaHCO3 0.84 g / L -1 0.01 gL NaH2PO4 -1 MgSO4·7H2O 0.0568 g L -1 CaCl2 0.0735 g L -1 ; The addition ratio of trace element solution I in wastewater is 1 mL / L. -1 ; Trace element solution I comprises the following components: EDTA 5.00 g / L -1 FeSO4 9.14 g L -1 ; The solvent for trace element solution I is distilled water; The addition ratio of trace element solution II in wastewater is 1 mL / L. -1 ; Trace element solution II comprises the following components: EDTA 15.0 g / L -1 ZnSO4·7H2O 0.430 g L -1 CoCl2·6H2O 0.240 g L -1 MnCl2·4H2O 0.990 g L -1 CuSO4·5H2O 0.250 g L -1 NaMoO4·2H2O 0.220 g L -1 NiCl2·6H2O 0.210 g L -1 H3BO4 0.014 g L -1 ; The solvent for Trace Element Solution II is distilled water.

5. The method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation according to claim 1, characterized in that, The added organic carbon source includes glucose.

6. The method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation according to claim 1, characterized in that, The concentration of the added organic carbon source was adjusted to 150 mg COD L. -1 Furthermore, it regulates the interaction between anaerobic ammonia oxidation granular sludge and heterotrophic nitrobenzene-degrading bacteria. Bacillus pumilus The NJUST51 mass ratio is 1:1, achieving the optimal state of simultaneous nitrogen removal and nitrobenzene removal.

7. The method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation according to claim 1, characterized in that, The composite biological system operates under conditions of avoiding light and 35±1℃.

8. The method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation according to claim 1, characterized in that, The pH of the wastewater is 7.2~7.

4.

9. The method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation according to claim 1, characterized in that, The method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation employs a batch reaction system.

10. The method for synergistic regulation of autotrophic anaerobic ammonium oxidation and heterotrophic nitrobenzene degradation according to claim 1, characterized in that, The initial concentration of nitrobenzene added to the wastewater shall not exceed 10 mg / L. -1 .

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