Method for promoting emergence rate and seedling stage growth of cotton under salt stress based on Mg / N NPs coating agent
By treating cotton seeds with Mg/N NPs coating agent, the problem of low germination rate under salt stress was solved, and the growth and stress resistance of cotton seedlings were significantly improved, the ROS content was reduced, and starch accumulation was promoted.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- INST OF COTTON RES CHINESE ACAD OF AGRI SCI
- Filing Date
- 2025-12-29
- Publication Date
- 2026-04-17
AI Technical Summary
Existing technologies result in low germination rates for cotton seeds under salt stress and are easily affected by the timing of field irrigation, making it difficult to effectively promote seedling growth.
Cotton seeds were treated with a Mg/N NPs coating agent, which consisted of a Mg/N NPs solution and a base solution, including chitosan powder, sodium dodecyl sulfate, 1-hexadecyl alcohol, and other components. The coating process improved the salt tolerance of the seeds.
It significantly improved the emergence rate and seedling growth of cotton under salt stress, enhanced the antioxidant capacity of seedlings, reduced ROS content, promoted starch accumulation, and improved the stress resistance of cotton.
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Figure CN121866928A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of cotton planting technology, specifically to a method for promoting cotton emergence rate and seedling growth under salt stress based on Mg / N NPs coating agent. Background Technology
[0002] Cotton growth is hampered by soil salinization, which damages seed cell structure, significantly reducing intracellular protein and fat content, thus leading to lower germination rates. Soil salinization also affects the growth of cotton. + When the concentration of solutes such as sodium is higher than that of root cell cytoplasm, an osmotic potential difference is created, leading to water loss in the plant and causing osmotic stress. This also forces the plant roots to absorb more sodium. + Osmotic and ion stress can disrupt ion balance and cause ion stress. Furthermore, osmotic and ion stress can lead to cell death, which generates and accumulates reactive oxygen species (ROS). Excessive ROS accumulation hinders the synthesis of phytochromes, reducing photosynthetic performance; it can also cause oxidative stress, malondialdehyde (MDA) accumulation, and membrane damage, potentially leading to electrolyte leakage and cell death in severe cases. Therefore, exploring ways to improve cotton emergence rate in saline-alkali soils is crucial for ensuring cotton production.
[0003] Nanobiotechnology offers new insights for sustainable agricultural development. Nanomaterials are defined as materials with at least one dimension between 1 and 100 nm in three-dimensional space. Research on nanomaterials improving crop germination rates under salt stress has been widely reported. Previous studies have shown that most nanoparticles (NPs) enhance seed germination rates and promote seedling growth through seed initiation, scavenging excessive ROS accumulation under salt stress, and increasing antioxidant enzyme activity. For example, seed initiation with SiO2 NPs, CeO2 NPs, and Ag NPs significantly improves the germination rates of wheat, rapeseed, and rice under salt stress, promoting root elongation and seedling growth. Biosynthesized Se NPs also regulate rapeseed's response to Na+. + and K + It increases the uptake of nutrients and the accumulation of osmotic substances, thereby significantly improving the germination rate and seedling growth of rapeseed.
[0004] Although numerous studies have explored using nanomaterials to improve seed salt tolerance through pretreatment, most have focused on soaking. This method, however, can lead to seed dehydration during the subsequent drying process. Furthermore, in field application, insufficient irrigation during emergence may inhibit seed germination. Therefore, how to pretreat cotton seeds with nanomaterials, ensuring that the treated seeds are unaffected by irrigation timing and improving emergence and seedling growth rates under salt stress, is a pressing issue for those skilled in the art. Summary of the Invention
[0005] To address the above problems, this invention provides a method for promoting cotton emergence rate and seedling growth under salt stress using a Mg / N NPs coating agent. The Mg / N NPs coating agent comprises a 1 g / L Mg / N NPs solution and a base solution in a ratio of (25-400) mL:1 L. The base solution comprises chitosan powder, sodium dodecyl sulfate, 1-hexadecyl alcohol, polyvinylpyrrolidone, glycerol, sodium benzoate, basic rose extract, hydrochloric acid solution, anhydrous ethanol, and water in a ratio of 0.5 g:0.5 g:0.025 g:1.25 g:0.25 g:0.05 g:0.25 g:10 mL:5 mL:25 mL.
[0006] Preferably, the hydrochloric acid solution is a hydrochloric acid solution with a volume fraction of 0.5%.
[0007] Preferably, the Mg / N NPs solution is prepared by mixing cotton leaf powder, urea, magnesium acetate tetrahydrate and water in a ratio of 0.242g:0.03g:0.214g:12.5mL, stirring at 500r / min for 30min, reacting at 180℃ for 12h, centrifuging at 8000r / min for 15min, and dialyzing through a MWCO1000 dialysis bag for 24h to obtain the Mg / N NPs solution.
[0008] Preferably, the preparation method of the base solution is as follows: Hydrochloric acid solution is heated to 65°C in a water bath, then chitosan powder is added and stirred at 500 r / min until dissolved to obtain solution A; 3 / 5 of the total volume of water is mixed with sodium dodecyl sulfate at 2000 r / min and shaken for 5 min to obtain solution B; 1-hexadecyl alcohol and anhydrous ethanol are mixed to obtain solution C; 2 / 5 of the total volume of water, polyvinylpyrrolidone, glycerol, sodium benzoate, and basic rose extract are taken, shaken to dissolve, and then solution D is obtained; solutions A, B, C, and D are completely mixed and stirred at 500 r / min for 30 min to obtain the base solution.
[0009] The method for promoting cotton emergence rate and seedling growth under salt stress based on Mg / N NPs coating agent is as follows: cotton seeds are mixed with 1% sodium hypochlorite solution at a volume fraction of 1g:5mL and soaked for 10min. After rinsing with water and drying, the cotton seeds are then coated with Mg / N NPs coating agent at a volume fraction of 100g:5mL before sowing.
[0010] Preferably, the cotton variety is one or more of Zhongmian 113, Zhongmian Institute 88, and Yuanmian 8.
[0011] The present invention has the following advantages:
[0012] (1) In the indoor experiment, after 15-21 days of salt stress, the seedling emergence rate of the treatment containing Mg / N NPs was the highest and significantly higher than that of the control group. Compared with the control group, after 21 days of salt stress, the Mg / N NPs coating agents all increased the fresh weight and dry weight of cotton seedlings, but the 5 mg / L Mg / N NPs treatment showed the greatest increase, which significantly promoted the growth of cotton seedlings under salt stress. In addition, compared with the control group, the 5 mg / L Mg / N NPs coating agent significantly reduced the H2O2 content and O2 content in the cotyledons after 21 days of salt stress. •- The Mg / N NPs coating significantly increased the activities of POD and CAT, but had no significant effect on SOD activity. Measurements of the osmotic regulator content in cotyledons showed that, compared to the Control group, the 5 mg / L Mg / N NPs coating significantly increased the starch content in the cotyledons.
[0013] (2) In saline-alkali cotton fields, compared with the control group, Mg / N NPs coating agent significantly improved the early and final emergence rates of saline-alkali land (salt content 6.2‰, pH 8.0) in Yuli County and saline-alkali land (salt content 6.1‰, pH 7.9) in Awati Township, Kashgar City after rehydration, and could significantly improve the plant height, fresh weight and dry weight of seedlings, and promote the growth and development of cotton seedlings.
[0014] (3) Mg / N NPs coating agent can improve the emergence rate, seedling height, fresh weight and dry weight of cotton under high salt stress, significantly increase the activity of POD and CAT in cotyledons, reduce ROS content, promote starch accumulation, alleviate the inhibition of cotton growth by salt stress, and improve the stress resistance of cotton under salt stress. Attached Figure Description
[0015] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.
[0016] Figure 1 Characterization of magnesium nitrogen-doped nanoparticles (Mg / N NPs).
[0017] Figure 2 The germination rate of cotton under salt stress in an indoor experiment.
[0018] Figure 3 This study investigated the effects of coating agents on cotyledon ROS content and antioxidant enzyme activity under salt stress in an indoor experiment.
[0019] Figure 4 This study investigated the effect of coating agents on the content of osmotic substances in cotyledons under salt stress in an indoor experiment.
[0020] Figure 5 The effect of Mg / N NPs coating agent on seedling emergence rate and seedling growth in saline-alkali cotton fields in Yuli County;
[0021] Figure 6 The effects of Mg / N NPs coating agent on seedling emergence rate and seedling growth in saline-alkali cotton fields in Awati Township. Detailed Implementation
[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0023] Example 1
[0024] Preparation of substrate solution:
[0025] 10 mL of 0.5% hydrochloric acid solution was heated to 65°C in a water bath, and then 0.5 g of chitosan powder was added. The mixture was stirred at 500 rpm until dissolved to obtain solution A. 15 mL of pure water and 0.5 g of sodium dodecyl sulfate were mixed and shaken at 2000 rpm for 5 min to obtain solution B. 0.025 g of 1-hexadecyl alcohol and 5 mL of anhydrous ethanol were mixed to obtain solution C. 10 mL of distilled water, 1.25 g of polyvinylpyrrolidone, 0.25 g of glycerol, 0.05 g of sodium benzoate, and 0.25 g of basic rose extract were shaken to dissolve the mixture to obtain solution D. Solutions A, B, C, and D were completely mixed and stirred at 500 rpm for 30 min to obtain the base solution.
[0026] Preparation of Mg / N NPs solution:
[0027] 0.242 g of dried cotton leaf powder, 0.03 g of urea, 0.214 g of magnesium acetate tetrahydrate, and 12.5 mL of ultrapure water were mixed, stirred at 500 rpm for 30 min, reacted at 180 °C for 12 h, centrifuged at 8000 rpm for 15 min, and dialyzed through a MWCO1000 dialysis bag for 24 h to obtain a Mg / N NPs solution, which was stored at 4 °C for later use. Characterization is shown in the figure. Figure 1 .Depend on Figure 1 It can be seen that Mg / N NPs are observed to be granular under a transmission electron microscope. Figure 1 The dark black substance in A was statistically analyzed and found to have a TEM particle size of 2.70±0.09nm. After measuring the potential, it was found that the surface of the material carried a negative charge, and the Zeta potential was 10.17±0.64mV.
[0028] Preparation of Mg / N NPs coating agent:
[0029] A 1 g / L Mg / N NPs solution was mixed with a base solution to achieve Mg / N NPs concentrations of 0, 2.5, 5, 10, 20, and 40 mg / L.
[0030] Experimental Example 1 (Indoor Experiment)
[0031] Cotton seeds of Zhongmian 113 were soaked in a 1% sodium hypochlorite solution for 10 minutes, rinsed with pure water, and dried. The seeds were then coated with the coating agent prepared in Example 1 (Mg / N NPs concentrations of 0, 2.5, 5, 10, 20, and 40 mg / L, respectively) (5 mL of coating agent per 100g of seeds). The coated cotton seeds were then dried for later use. Soil (nutrient soil: vermiculite = 1:1) was placed in germination boxes (19×13×12 cm), with 15 cotton seeds in each box. The boxes were then irrigated with NaCl solution to achieve a final NaCl content of approximately 7.5‰. The germination rate was assessed every other day, and samples were taken on day 21 to measure relevant indicators. On day 21, the seedlings in each box were cleaned, dried, and weighed fresh. They were then blanched at 105℃ for 30 minutes and dried at 80℃ to constant weight. The dry weight of the seedlings was recorded.
[0032] This experiment included two treatments: a coating agent without Mg / N NPs (Control) and a coating agent containing 5 mg / L Mg / N NPs (Mg / N NPs). Each treatment was repeated six times. The results are shown in [Figure Number]. Figure 2-4 .
[0033] Depend on Figure 2-4 It can be seen that coating agents containing Mg / N NPs can uniformly coat the surface of cotton seeds. Figure 2 A). Concentration screening experiments showed that after 15-21 days of salt stress, the seedling emergence rate was highest in the 5 mg / L Mg / N NPs coating treatment, and significantly higher than that in the Control treatment. Figure 2 B and 2E). Compared with the control group, after 21 days of salt stress, different concentrations of Mg / N NPs coating agents all increased the fresh weight (11.11%-23.03%) and dry weight (18.00%-38.00%) of cotton seedlings, but the 5 mg / L Mg / N NPs treatment showed the greatest increase, with increases of 23.03% and 38.00% in fresh weight and dry weight, respectively. This treatment significantly promoted the growth of cotton seedlings under salt stress. Figure 2 (C, 2D and 2F).
[0034] After 21 days of salt stress, compared with the control group, the 5 mg / L Mg / N NPs coating agent significantly reduced the H2O2 content (14.10%) and O2 content in cotyledons. •-The content of Mg / N NPs coating agent was significantly increased (26.92%), significantly improving the activities of POD (425.49%) and CAT (423.56%), but had no significant effect on SOD activity. The determination of osmotic regulators in cotyledons showed that, compared with the Control, 5 mg / L Mg / N NPs coating agent significantly increased the starch content in cotyledons (17.06%), but had no significant effect on protein concentration, malondialdehyde content, or soluble sugar content.
[0035] In summary, the 5 mg / L Mg / N NPs coating agent showed the greatest improvement in emergence rate, fresh weight, and dry weight compared to the control group. Furthermore, this treatment significantly increased the activities of POD and CAT in cotton cotyledons, enhanced the cells' ability to scavenge ROS, and reduced the excessive accumulation of H2O2 and O2 under salt stress. •- The content promotes starch accumulation, thereby improving the salt tolerance of cotton and promoting seedling growth.
[0036] Experimental Example 2 (Field Experiment)
[0037] Field experiment in Yuli County (salt content 6.2‰, pH 8.0): Included two treatments: uncoated and treated with 5 mg / L Mg / N NPs coating agent (Mg / N NPs), each treatment covering 0.5 mu (approximately 0.067 hectares). Each treatment was replicated five times during the survey. Sowing was on April 9th, and re-irrigation was on April 16th. A machine-harvested cotton planting pattern of six rows per film (66+10 cm) was adopted. Fertilization, irrigation, and other field management measures were carried out according to local cotton field management requirements.
[0038] Field experiment in Awat Township, Kashgar City (salt content 6.1‰, pH 7.9): Two treatments were included: uncoated and coated with 5 mg / L Mg / N NPs (Mg / N NPs). Each treatment covered 1.0 mu (approximately 0.067 hectares), and each treatment was replicated four times during the investigation. Sowing was on April 15th, and re-irrigation was on April 16th. A machine-harvested cotton planting pattern of four rows per film (76+10+66) cm was adopted. Fertilization, irrigation, and other field management measures were carried out according to local cotton field management requirements.
[0039] In Yuli County, the field emergence rate of saline-alkali land was investigated at 10 and 35 days after re-watering; in Awat Township, Kashgar City, the field emergence rate was investigated at 12 and 34 days after re-watering. The results are shown below. Figure 5-6 .
[0040] Depend on Figure 5-6 It was found that, compared with the control group, the 5 mg / L Mg / N NPs coating agent significantly improved the seedling emergence rate of saline-alkali land in Yuli County 10 days and 35 days after re-watering, increasing it by 24.03% and 15.37%, respectively. Figure 5A). Furthermore, compared to the control group, the coating agent containing Mg / N NPs promoted the growth and development of cotton seedlings, specifically by significantly increasing seedling height, true leaf number, fresh weight, and dry weight by 17.63%, 12.70%, 21.33%, and 15.38%, respectively. Figure 5 (D, E, and F).
[0041] In saline-alkali cotton fields in Awat Township, Kashgar, compared with the control group, the 5 mg / L Mg / N NPs coating agent significantly increased the emergence rate at 12 days and 34 days after rehydration, by 41.18% and 9.37%, respectively. Figure 6 A). Furthermore, compared to the control group, the coating agent containing Mg / N NPs promoted the growth and development of cotton seedlings, specifically by significantly increasing seedling height, fresh weight, and dry weight by 25.90%, 20.65%, and 19.18%, respectively. Figure 6 D and F), but had no significant effect on the number of true leaves during the seedling stage ( Figure 6 E).
[0042] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A Mg / N NPs coating agent characterized in that, The mixture includes a 1 g / L Mg / N NPs solution and a base solution, such that the Mg / N NPs concentration is 2.5-40 mg / L. The base solution comprises chitosan powder, sodium dodecyl sulfate, 1-hexadecyl alcohol, polyvinylpyrrolidone, glycerol, sodium benzoate, basic rose extract, hydrochloric acid solution, anhydrous ethanol, and water in a ratio of 0.5 g: 0.5 g: 0.025 g: 1.25 g: 0.25 g: 0.05 g: 0.25 g: 10 mL: 5 mL: 25 mL.
2. The Mg / N NPs coating agent according to claim 1, characterized in that, The hydrochloric acid solution has a volume fraction of 0.5%.
3. The Mg / N NPs coating agent according to claim 1, characterized in that, The Mg / N NPs solution was prepared by mixing cotton leaf powder, urea, magnesium acetate tetrahydrate and water in a ratio of 0.242g:0.03g:0.214g:12.5mL, stirring at 500r / min for 30min, reacting at 180℃ for 12h, centrifuging at 8000r / min for 15min, and dialyzing at 1000Da for 24h to obtain the Mg / N NPs solution.
4. The Mg / N NPs coating agent according to claim 1, characterized in that, The method for preparing the base solution is as follows: heating the hydrochloric acid solution to 65°C in a water bath, then adding chitosan powder, and stirring at 500 r / min until dissolved to obtain solution A; Take 3 / 5 of the total volume of water and mix with sodium dodecyl sulfate at 2000 r / min and shake for 5 min to obtain solution B; mix 1-hexadecyl alcohol and anhydrous ethanol to obtain solution C; take 2 / 5 of the total volume of water, polyvinylpyrrolidone, glycerol, sodium benzoate and basic rose essence, shake to dissolve and obtain solution D. Mix solutions A, B, C, and D thoroughly and stir at 500 rpm for 30 minutes to obtain the base solution.
5. A method of promoting seedling emergence rate and seedling growth under salt stress in cotton using Mg / N NPs coating agent as claimed in any one of claims 1-4, characterized in that, Cotton seeds were coated with Mg / N NPs coating agent at a ratio of 100g:5mL before sowing.
6. The method according to claim 5, characterized in that, The volume fraction of the sodium hypochlorite solution is 1%.
7. The method according to claim 5, characterized in that, The cotton varieties mentioned are one or more of Zhongmian 113, Zhongmian Institute No. 88, and Yuanmian No. 8.