Anti-alcoholism composition and application thereof

Abstract

The invention provides an anti-alcoholism composition and application thereof, the anti-alcoholism composition comprises an anti-alcoholism enzyme preparation and a bioactive preparation, the anti-alcoholism enzyme preparation comprises at least one of ethanol dehydrogenase and acetaldehyde dehydrogenase, the bioactive preparation comprises a product obtained by enzymolysis of protein contained in micrococcus, and the product comprises polypeptide substances. According to the disclosure, the invention can provide the anti-alcohol composition which can effectively relieve the burden caused by the entry of ethanol or metabolites thereof into the liver and has less side effects and the application of the anti-alcohol composition.

Description

Technical Field

[0001] This disclosure relates to the biopharmaceutical industry, specifically to a hangover remedy composition and its application. Background Technology

[0002] Alcohol is a common beverage in daily social and lifestyle settings. After ingestion, it typically undergoes the following metabolic process: first, it is converted into toxic acetaldehyde under the catalysis of alcohol dehydrogenase (ADH), then further broken down into acetic acid under the catalysis of aldehyde dehydrogenase (ALDH), and finally excreted as carbon dioxide and water. When alcohol intake is excessive or the body's own ADH and ALDH activity is insufficient, acetaldehyde tends to accumulate in the body, causing symptoms of intoxication such as headache, nausea, and fatigue. Long-term excessive drinking further increases the metabolic burden on the liver and may even induce liver damage. Therefore, developing safe and effective hangover remedies has become a key demand for mitigating the harms of alcohol consumption.

[0003] Currently, common methods for relieving hangovers include dietary therapy, physical methods, and medications. Dietary therapy includes honey water: fructose promotes alcohol dehydrogenase activity, accelerating ethanol metabolism, but a large intake of fructose is required for it to be effective; kudzu root extract: puerarin increases alcohol dehydrogenase activity by dilating liver blood vessels, thus increasing the rate of ethanol metabolism; dairy products: whey protein in milk forms a protective layer on the gastric mucosa, slowing down ethanol absorption. Physical methods include: acupressure to improve post-drinking discomfort (such as vomiting); warm water baths to promote ethanol excretion through the skin, etc. Medications include chemical drugs such as naloxone and metadoxine, and biological agents such as B vitamins.

[0004] However, among the methods mentioned above, dietary therapy and hangover remedies usually require the active substances in the hangover remedies to be absorbed by the body before they can take effect, making it difficult to significantly reduce the burden caused by ethanol or its metabolites entering the liver; while hangover remedies, such as chemical drugs, may have side effects or cause gastrointestinal discomfort, increased liver load, etc. Summary of the Invention

[0005] This disclosure was made in view of the above-mentioned state of the prior art, and its purpose is to provide a hangover relief composition and its application that can effectively reduce the burden caused by ethanol or its metabolites entering the liver and has fewer side effects.

[0006] Therefore, the first aspect of this disclosure provides a hangover remedy composition, comprising a hangover-degrading enzyme preparation and a bioactive preparation. The hangover-degrading enzyme preparation includes at least one of alcohol dehydrogenase and aldehyde dehydrogenase, and the bioactive preparation includes a product obtained by enzymatic hydrolysis of proteins contained in *Micrococcus pluvialis*, the product comprising polypeptides. In the hangover remedy composition of this disclosure, an exogenous hangover-degrading enzyme preparation is added. When taken orally, the hangover-degrading enzyme preparation can break down ethanol in the digestive tract (gastrointestinal tract); while the bioactive preparation can enhance the activity of the hangover-degrading enzyme (alcohol dehydrogenase or aldehyde dehydrogenase) in the digestive tract, further promoting the breakdown of ethanol. Thus, by metabolizing ethanol in the digestive tract, the amount of ethanol entering the liver can be reduced, alleviating the burden on the liver and achieving the hangover remedy effect. Furthermore, the polypeptides have a small molecular weight and can enter the bloodstream, further enhancing the activity of the hangover-degrading enzymes in the blood and promoting the breakdown of ethanol. Moreover, the bioactive preparation is derived from natural biological raw materials, has good biocompatibility, and few side effects. It should be noted that *Nannochloropsisgaditana* has received considerable attention in recent years due to its rich bioactive components (unsaturated fatty acids, antioxidants, polysaccharides, etc.). The applicant discovered that the product obtained by enzymatic hydrolysis of proteins extracted from *Nannochloropsisgaditana* can effectively enhance the activity of alcohol-degrading enzymes through a specific mechanism of action, thereby accelerating the metabolism of ethanol.

[0007] In the hangover relief composition disclosed herein, optionally, the hangover-relieving enzyme preparation is fermented vinegar powder, and the mass ratio of the fermented vinegar powder to the bioactive preparation in the hangover relief composition is 1:1 to 3:1. This further improves the activity of the hangover-relieving enzyme and enhances its ability to break down ethanol.

[0008] In the hangover relief composition disclosed herein, optionally, the ratio of alcohol dehydrogenase activity units (IU) to acetaldehyde dehydrogenase activity units (IU) in the fermented vinegar powder is 1:1 to 1:3. In this case, it is beneficial to balance the decomposition capabilities of ethanol and acetaldehyde, thus promoting detoxification.

[0009] In the hangover relief composition disclosed herein, optionally, the microorganisms include at least one of *Acetobacter pastoris*, lactic acid bacteria, and *Bacillus subtilis*, wherein the lactic acid bacteria include at least one of *Lactobacillus helveticus*, *Lactobacillus plantarum*, and *Streptococcus thermophilus*. Thus, fermented vinegar powder containing well-active alcohol dehydrogenase and acetaldehyde dehydrogenase can be obtained.

[0010] In the hangover-relieving composition disclosed herein, optionally, the enzyme used for enzymatic hydrolysis includes at least one of human or animal-derived proteases, plant proteases, and microbial proteases. The human or animal-derived proteases include at least one of salivary protease, trypsin, pepsin, and chymotrypsin. The plant proteases include at least one of papain and bromelain, and the microbial proteases include at least one of subtilisin and Aspergillus protease. This further enhances the effect of polypeptides on the activity of hangover-relieving enzymes.

[0011] Optionally, in the hangover relief composition disclosed herein, the total peptide content of the bioactive preparation exceeds 60%. In this case, the higher proportion of polypeptides and the lower proportion of impurities facilitate the interaction of more effective peptides with the hangover-degrading enzymes, thereby enhancing the activity of the hangover-degrading enzymes and reducing the side effects or metabolic burden caused by impurities on the human body.

[0012] Optionally, in the hangover relief compositions disclosed herein, the hangover relief composition meets at least one of the following conditions: the hangover relief composition promotes the breakdown of ethanol in the digestive tract, blood, or tissues; the hangover relief composition is taken orally 5 to 30 minutes before or after drinking alcohol; each dose of the hangover relief composition contains 0.1 g to 1 g of the bioactive preparation; the hangover relief composition is in the form of at least one of compressed candy, solid beverage, liquid beverage, and yogurt. In this case, by metabolizing ethanol in the digestive tract, the amount of ethanol entering the liver can be reduced, alleviating the burden on the liver and achieving the effect of relieving hangovers.

[0013] The second aspect of this disclosure provides the use of the hangover relief composition as provided in the first aspect of this application in relieving hangovers and / or preparing hangover relief products.

[0014] According to this disclosure, a hangover relief composition and its application can be provided that can effectively reduce the burden on the liver caused by ethanol or its metabolites entering the liver, and has fewer side effects. Attached Figure Description

[0015] Figure 1 This is a flowchart illustrating a method for preparing a bioactive formulation according to an example of this disclosure.

[0016] Figure 2 This is a flowchart illustrating a method for preparing a bioactive formulation according to another example of this disclosure.

[0017] Figure 3 This is a graph showing the experimental results of the hangover relief effect of the compound enzyme powder involved in this disclosure.

[0018] Figure 4This is a graph showing the results of an experiment on the hangover relief effect of the hangover relief composition of Example 1, which is an example of this disclosure.

[0019] Figure 5 This is a graph showing the results of an experiment on the hangover relief effect of the hangover relief composition of Example 2, which is an example of this disclosure.

[0020] Figure 6 This is a graph showing the results of ethanol metabolism rate tests for different products involved in the examples of this disclosure.

[0021] Figure 7 This is a graph showing the results of a test of the perceived effectiveness of the hangover relief composition of Example 1, which is an example of this disclosure.

[0022] Figure 8 This is a diagram showing the results of a breathalyzer test after using the hangover relief composition of Example 1, which is an example of this disclosure. Detailed Implementation

[0023] All references cited in this disclosure are incorporated herein by reference in their entirety, as fully illustrated. Unless otherwise defined, the technical and scientific terms used in this disclosure have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure pertains.

[0024] Hereinafter, preferred embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. In the following description, the same reference numerals will be used for the same components, and repeated descriptions will be omitted. Furthermore, the drawings are merely schematic diagrams, and the proportions of the components or the shapes of the components may differ from actual figures.

[0025] In related technologies, the bioactive substances contained in hangover remedies need to be absorbed by the body before they can promote the production or activity of alcohol dehydrogenase or aldehyde dehydrogenase, making it difficult to minimize the burden on the liver caused by ethanol or its metabolites. These products are designed to protect the liver and are unlikely to directly metabolize ethanol before it enters the liver. This application provides a hangover remedy composition, a bioactive preparation, and its application. The hangover remedy composition of this application can break down ethanol or its metabolites before they enter the liver, thereby effectively reducing the burden on the liver caused by ethanol and its metabolites. It should be noted that "hangover remedy" can include at least one of the following: reducing or delaying the absorption of ethanol in the body; increasing the content or activity of alcohol dehydrogenase or aldehyde dehydrogenase to accelerate the ethanol metabolism process; and promoting the decomposition and excretion of alcohol (ethanol) and its toxic metabolite acetaldehyde.

[0026] This embodiment provides a hangover remedy composition.

[0027] In some examples, the hangover remedy composition may include a hangover-degrading enzyme preparation and a bioactive preparation. The hangover-degrading enzyme preparation promotes the metabolism of ethanol, while the bioactive preparation enhances the activity of the hangover-degrading enzyme.

[0028] In some examples, the alcohol-degrading enzyme preparation may include at least one of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). ADH catalyzes the conversion of ethanol to acetaldehyde. ALDH catalyzes the conversion of acetaldehyde to acetic acid. Acetaldehyde is a harmful substance, and ALDH can promote its detoxification. The bioactive preparation of this application, by enhancing the activity of alcohol-degrading enzymes, can facilitate the acceleration of the ethanol metabolic chain, reduce the accumulation of ethanol or acetaldehyde in the body, thereby achieving the effect of detoxification. In some examples, the alcohol-degrading enzyme preparation may include alcohol dehydrogenase and aldehyde dehydrogenase. Therefore, it is beneficial to simultaneously promote the conversion of ethanol and acetaldehyde.

[0029] In some examples, the alcohol-degrading enzyme preparation can be fermented vinegar powder. Fermented vinegar powder can be a fermentation product produced by microorganisms capable of metabolizing ethanol using ethanol as a substrate. The microorganisms contain alcohol dehydrogenase and acetaldehyde dehydrogenase. The microorganisms can include at least one of *Acetobacter pastoris*, lactic acid bacteria, and *Bacillus subtilis*. The lactic acid bacteria can include at least one of *Lactobacillus helveticus*, *Lactobacillus plantarum*, and *Streptococcus thermophilus*.

[0030] In some examples, the mass ratio of the alcohol-degrading enzyme preparation to the bioactive preparation in the hangover-relieving composition can be 1 to 3:1. For example, the mass ratio of the alcohol-degrading enzyme preparation to the bioactive preparation in the hangover-relieving composition can be 1:1, 1.5:1, 2:1, 2.5:1, or 3:1. This can further improve the activity of the alcohol-degrading enzyme and enhance its ability to break down ethanol. In some examples, the mass ratio of the fermented vinegar powder to the bioactive preparation can be 1 to 3:1. For example, the mass ratio of the fermented vinegar powder to the bioactive preparation in the hangover-relieving composition can be 1:1, 1.5:1, 2:1, 2.5:1, or 3:1.

[0031] In some examples, the ratio of alcohol dehydrogenase activity units (IU) to acetaldehyde dehydrogenase activity units (IU) in alcohol-degrading enzyme preparations can be 1:1 to 3. For example, the ratio of alcohol dehydrogenase activity units to acetaldehyde dehydrogenase activity units can be 1:1, 1:1.5, 1:2, 1:2.5, or 1:3. This helps to balance the ability to break down ethanol and acetaldehyde, thus promoting detoxification.

[0032] In some examples, the bioactive preparation may include products obtained by enzymatic hydrolysis of proteins contained in *Chlorella pseudocaryophyllum*. It should be noted that the applicant has discovered that products obtained by enzymatic hydrolysis of proteins from *Chlorella pseudocaryophyllum* can effectively enhance the activity of alcohol-degrading enzymes through a specific mechanism of action, thereby accelerating ethanol metabolism. In some examples, the bioactive preparation may include products obtained by enzymatic hydrolysis of culture media containing *Chlorella pseudocaryophyllum*, *Chlorella pseudocaryophyllum* powder, or proteins contained in *Chlorella pseudocaryophyllum*. In other words, enzymatic hydrolysis can be performed on culture media containing *Chlorella pseudocaryophyllum*, *Chlorella pseudocaryophyllum* powder, or proteins contained in *Chlorella pseudocaryophyllum* to obtain enzymatic hydrolysates, and the bioactive preparation includes these enzymatic hydrolysates.

[0033] In some examples, the enzymes used for enzymatic hydrolysis may include at least one of human or animal-derived proteases, plant proteases, and microbial proteases. Human or animal-derived proteases may include at least one of salivary proteases, trypsin, pepsin, and chymotrypsin. Plant proteases may include at least one of papain and bromelain. Microbial proteases may include at least one of subtilisin and Aspergillusin. Preferably, the enzymes used for enzymatic hydrolysis include at least one of alkaline proteases and papain. This further facilitates the obtaining of small molecule peptides with good hangover-relieving properties. In some examples, enzymatic hydrolysis under appropriate conditions can hydrolyze large molecule proteins in *Micrococcus pluvialis* into water-soluble small molecule peptides.

[0034] In some examples, the products of enzymatic hydrolysis may include polypeptides. In other words, bioactive preparations may include polypeptides. In some examples, polypeptides may also be called "microalgae bioactive peptides". Polypeptides are small molecules formed by 2 to 50 amino acids linked by peptide bonds. Polypeptides have no complex spatial folding (or only simple folding) and have a small molecular weight (usually below 10 kDa). As a component of hangover relief compositions, polypeptides have the following advantages: (1) Easy absorption: Due to their small molecular weight, they do not need to be completely digested and broken down by the gastrointestinal tract like proteins. They can be directly absorbed into the bloodstream by the intestines and exert biological activity quickly, which is suitable for the need for rapid hangover relief after drinking. (2) Specific activity: Polypeptides with specific amino acid sequences can specifically bind to biological targets (such as the active center of ADH enzyme or ALDH enzyme in this application), activate enzyme activity, and have few side effects and good biocompatibility. (3) Good stability: Compared with amino acids, peptide bond structure is more stable and is not easily degraded during storage or digestion; compared with proteins, it will not lose activity due to complex spatial structure, and is suitable for the development of multiple dosage forms.

[0035] In some examples, peptides can enhance the activity of alcohol-degrading enzymes through specific amino acid arrangements and spatial structures. For instance, the hydrophobic-neutral-hydrophilic complex structure of "leucine-alanine-glutamic acid" or "valine-isoleucine-glutamic acid" allows the hydrophobic end to bind to the hydrophobic region of the active site of the alcohol-degrading enzyme, stabilizing the enzyme conformation; the hydrophilic end binds to the substrate / coenzyme through hydrogen bonds / electrostatic interactions, which can accelerate the catalytic reaction; and the neutral region provides structural flexibility, making the peptide more adaptable to the conformation of the active site of the alcohol-degrading enzyme. In some examples, peptides can enhance the activity of alcohol-degrading enzymes through metabolic synergy. For example, the metabolic processes of some peptides can generate ATP to provide energy for the catalytic reaction of alcohol-degrading enzymes. In some examples, peptides can enhance the activity of alcohol-degrading enzymes through specific amino acid compositions, such as branched-chain amino acids—leucine, isoleucine, and valine, or alanine and glutamic acid. In some examples, peptides can enhance the activity of alcohol-degrading enzymes through specific amino acid ratios.

[0036] In some examples, the molecular weight of the peptide can be between 0.5 kDa and 3 kDa. For example, the molecular weight of the peptide can be 0.5 kDa, 1 kDa, 1.5 kDa, 2 kDa, 2.5 kDa, or 3 kDa. In some examples, the peptide can be 2 to 10 peptides. In some examples, the molecular weight of the peptide can be between 1 kDa and 2 kDa. In these cases, the smaller molecular weight of the peptide allows it to enter the bloodstream, increasing the activity of endogenous alcohol-degrading enzymes (alcohol dehydrogenase, aldehyde dehydrogenase), continuing to metabolize alcohol in the blood and tissues, reducing the amount of ethanol and acetaldehyde entering the liver, and mitigating the damage caused by ethanol and acetaldehyde to the liver.

[0037] In some examples, the polypeptide may include, based on its amino acid composition: 6%–7% L-aspartic acid, 3%–3.5% L-threonine, 2.5%–3% L-serine, 8.5%–9.5% L-glutamic acid, 2.5%–3.5% L-proline, 3.5%–4.5% glycine, 4.5%–5.5% L-alanine, 3.5%–4.5% L-valine, 1%–1.5% L-methionine, 2.5%–3.5% L-isoleucine, 5.5%–6.5% L-leucine, 2%–2.5% L-tyrosine, 3%–3.5% L-phenylalanine, 4%–5% L-lysine, 0.5%–1.5% L-histidine, and 3.5%–4.5% L-arginine.

[0038] In some examples, the product may also include free amino acids. In other words, the bioactive formulation may also include free amino acids. In some examples, free amino acids may have the effect of enhancing the activity of alcohol-degrading enzymes. In some examples, the product may include more than 70% polypeptides and less than 20% free amino acids.

[0039] In some examples, *Micrococcus pseudochlorella* or bioactive preparations can be treated to remove impurities. This reduces impurities in the bioactive preparations, increases the proportion of peptides, facilitates the interaction of more effective peptides with alcohol-degrading enzymes to enhance enzyme activity, and reduces the side effects or metabolic burden caused by impurities on the human body.

[0040] In some examples, the purification process may include at least one of desalting, defatting, nucleic acid removal, polysaccharide removal, heavy metal removal, and sterilization.

[0041] In some examples, *Chlorella vulgaris* can be desalted first to obtain polypeptides. This reduces the inorganic salt content in bioactive preparations, increases the proportion of polypeptides, facilitates the interaction of more effective peptides with alcohol-degrading enzymes, enhances enzyme activity, and reduces the side effects or metabolic burden caused by impurities.

[0042] In some examples, *Micrococcus pseudochlorella* can be degreased first to obtain polypeptides. This reduces the oil content in bioactive preparations, increases the proportion of polypeptides, facilitates the interaction of more effective peptides with alcohol-degrading enzymes, enhances enzyme activity, and reduces the side effects or metabolic burden caused by impurities.

[0043] In some examples, the total protein content (by mass) in the bioactive formulation exceeds 70%. For instance, the total protein content in the bioactive formulation can be 70%, 75%, 80%, 85%, 90%, or 95%. In some examples, the total protein content in the bioactive formulation can exceed 85%. This ensures a sufficient amount of peptides.

[0044] It should be noted that, unless otherwise specified, the content in this application refers to the percentage by mass.

[0045] In some examples, the total peptide content (by mass) in the bioactive formulation can exceed 60%. For instance, the total peptide content in the bioactive formulation can be 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%. In some examples, the total peptide content in the bioactive formulation can exceed 70%. This allows for a higher content of polypeptides to ensure an effective enhancement of the activity of alcohol-degrading enzymes.

[0046] In some examples, the total peptide content in the bioactive formulation can exceed 70%, while the total protein content does not exceed 90%. This ensures a sufficient amount of polypeptides and a small amount of undigested protein, giving the bioactive formulation good absorption and hangover-relieving activity. In this application, the bioactive formulation may also be referred to as peptide powder or *Micrococcus pseudocarpa* peptide powder.

[0047] In some examples, the polysaccharide content (mass percentage) in the bioactive preparation can be less than 10%. For example, the polysaccharide content in the bioactive preparation can be 0%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%. In some examples, the polysaccharide content in the bioactive preparation can be less than 8%. In some examples, the polysaccharide content in the bioactive preparation can be less than 6%. It should be noted that polysaccharides are natural macromolecules in *Micrococcus pluvialis*, including algal polysaccharides, cellulose, etc. Polysaccharides may have the following effects: (1) Water solubility and hygroscopicity: Most polysaccharides are highly water-soluble and hygroscopic. Excessive content will cause the polypeptide product to easily absorb moisture and clump, affecting the product performance. (2) Interference effect: The macromolecular structure of polysaccharides may encapsulate polypeptides or compete with polypeptides for the solubility environment, affecting the binding of polypeptides with key enzymes for detoxification, reducing enzyme activation efficiency, and thus weakening the detoxification effect. Therefore, controlling the polysaccharide content is beneficial to ensuring the detoxification efficacy of the bioactive preparation.

[0048] In some examples, the nucleic acid content (mass percentage) in the bioactive formulation may be less than 2%. For example, the nucleic acid content in the bioactive formulation may be 0%, 0.5%, 1%, 1.5% or 2%. In some examples, the nucleic acid content in the bioactive formulation may be less than 1.5%. It should be noted that nucleic acids may include at least one of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Nucleic acids may have the following effects: (1) High water solubility: Nucleic acids have good water solubility, similar to polysaccharides, which will increase the hygroscopicity of peptide powder, aggravate the problems of aggregation and clumping, and affect the storage stability of the product. (2) Interference with product performance: The macromolecular structure of nucleic acids may form complexes with peptides or occupy the active binding sites of enzymes, reduce the activation efficiency of peptides on key enzymes for detoxification, and indirectly weaken the detoxification effect; at the same time, nucleic acid residues may also increase the ash-related risks of the product and affect the quality.

[0049] In some examples, the ash content (mass percentage) in bioactive preparations can be less than 10%. For example, the ash content in bioactive preparations can be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%. In some examples, the ash content in bioactive preparations can be less than 7%. It should be noted that ash content is an important indicator for measuring product purity. It refers to the percentage of the mass of non-flammable impurities such as inorganic minerals and metal oxides remaining after the sample has been burned and oxidized at high temperature (usually 550℃~800℃) to the total mass of the original sample, reflecting the amount of "non-organic impurities" in the product. In this application, ash content may have the following effects: (1) Affecting product quality: Excessive ash content will cause the peptide powder to have a dark color and a rough taste, which does not meet the purity requirements of food / health products and reduces the market acceptance of the product. (2) Increasing the burden on the body: The residual inorganic impurities need to be metabolized and excreted by the liver, which will increase the burden on the liver after drinking alcohol and partially offset the liver protection effect of peptides. (3) Impact on stability: Some inorganic impurities may interact with peptides or accelerate the absorption of moisture and aggregation of peptide powder, indirectly affecting the stability of the peptides' hangover-relieving activity. Therefore, controlling the ash content is beneficial to ensuring the hangover-relieving efficacy of bioactive preparations.

[0050] In some examples, at least one of the hangover-relieving enzyme preparation and the bioactive preparation in the hangover-relieving composition can be coated. The coating can include at least one of the following: gastric-soluble coating and enteric-soluble coating. The gastric-soluble coating is configured to dissolve rapidly in the acidic environment of the stomach (pH 1-3) without affecting the release of the contents within the stomach. The material of the gastric-soluble coating can include at least one of hydroxypropyl methylcellulose and povidone. The enteric-soluble coating is configured to dissolve and release the contents in the intestinal environment (pH 5.5 or higher). The enteric-soluble coating can include at least one of cellulose acetate phthalate and hydroxypropyl methylcellulose phthalate. Thus, the active ingredients of the hangover-relieving composition can be protected by coating, and the coating also provides certain functions such as light protection, moisture protection, and coloring.

[0051] In some examples, the hangover relief composition may also include excipients. In some examples, excipients may include at least one of diluents, excipients, binders, fillers, solubilizers, sustained-release agents, flavoring agents, and sweeteners. Thus, excipients can play roles such as diluting the active ingredient, excipients, binders, fillers, solubilizers, sustained-release agents, flavoring agents, or sweeteners, which can help improve the performance of the hangover relief composition and make it better meet user needs.

[0052] In some examples, the dosage form of the hangover remedy can be selected from one of the following: lozenges, powders, capsules, tablets, suspensions, emulsions, solutions, and syrups. This allows for the selection of different dosage forms according to actual needs.

[0053] In some examples, the hangover relief composition may be in the form of at least one of compressed candy, solid beverage, liquid beverage, and yogurt.

[0054] In some examples, the hangover relief composition promotes the breakdown of ethanol within the digestive tract.

[0055] In some examples, the hangover remedy can be taken orally.

[0056] In some examples, the hangover remedy can be taken before, during, or after drinking alcohol. In other examples, it can be taken 5 to 30 minutes before drinking alcohol. This allows the hangover remedy to first enter the gastrointestinal tract orally and then exert its effect there.

[0057] In some examples, the dosage of the hangover remedy composition can be 0.5g to 5g per dose. In other examples, the dosage can be 0.1g to 1g of a bioactive agent. For example, the dosage can be 0.1g, 0.2g, 0.3g, 0.4g, 0.5g, 0.6g, 0.7g, 0.8g, 0.9g, or 1g of a bioactive agent. In some examples, the dosage can be 0.2g to 2g of a hangover-degrading enzyme. For example, the dosage can be 0.2g, 0.4g, 0.6g, 0.8g, 1g, 1.2g, 1.4g, 1.6g, 1.8g, or 2g of a hangover-degrading enzyme. This further helps to ensure a good hangover-reducing effect.

[0058] This embodiment also provides a bioactive preparation. The bioactive preparation includes polypeptides, which are obtained by enzymatic hydrolysis of proteins extracted from *Micrococcus pseudocarpa*. The components of *Micrococcus pseudocarpa* can be referred to the description in the aforementioned hangover remedy composition, and will not be repeated here.

[0059] This embodiment also provides a method for preparing a hangover relief composition. The method for preparing the hangover relief composition includes: mixing a hangover-relieving enzyme preparation and a bioactive preparation in a predetermined ratio to obtain the hangover relief composition.

[0060] This embodiment also provides a method for preparing a bioactive preparation.

[0061] Figure 1 This is a flowchart illustrating a method for preparing a bioactive formulation according to an example of this disclosure.

[0062] In some examples, the preparation method of the bioactive agent may include: defatting treatment (step S10), enzymatic hydrolysis treatment (step S20) and purification treatment (step S30).

[0063] In some examples, step S10 may include: using *Nannochloropsis gaditana* as raw material, extracting algal oil with an extractant to obtain defatted *Nannochloropsis gaditana*. It should be noted that defatting can improve the efficiency of subsequent protein recovery.

[0064] In some examples, step S10 may include: soaking dried *Micrococcus pseudocarpa* algae in an extractant for a first preset time at a first preset temperature to obtain an extract and a residue, wherein the residue is defatted *Micrococcus pseudocarpa*.

[0065] In some examples, in step S10, the extractant can be an ethanol solution with a mass percentage of 95% to 100%. For example, the extractant can be 95% ethanol, 96% ethanol, 97% ethanol, 98% ethanol, 99% ethanol, or pure ethanol. In some examples, in step S10, the first preset temperature can be 65℃ to 80℃. For example, the first preset temperature can be 65℃, 68℃, 70℃, 72℃, 74℃, 76℃, 78℃, or 80℃. In some examples, in step S10, the first preset duration can be 0.5 hours to 2 hours. For example, the first preset duration can be 0.5 hours, 0.6 hours, 0.7 hours, 0.8 hours, 0.9 hours, 1 hour, 1.1 hours, 1.2 hours, 1.3 hours, 1.4 hours, 1.5 hours, 1.6 hours, 1.7 hours, 1.8 hours, 1.9 hours, or 2 hours. In some examples, in step S10, the amount of extractant used can be 5 to 12 times the mass of the dried *Micrococcus pseudocarpa*. For example, in step S10, the amount of extractant used can be 5, 6, 7, 8, 9, 10, 11, or 12 times the mass of the dried *Micrococcus pseudocarpa*.

[0066] In some examples, step S10 may include: subjecting the Chlorella pseudochlorella culture medium to desalination to obtain desalted Chlorella pseudochlorella; and drying the desalted Chlorella pseudochlorella to obtain dried Chlorella pseudochlorella algae.

[0067] In some examples, desalination of the *Chlorella pseudocarpa* culture medium may include: concentrating the *Chlorella pseudocarpa* culture medium by centrifugation or membrane filtration to obtain a concentrate with a dry weight percentage of 5% to 15%; and repeatedly washing and centrifuging the concentrate to obtain *Chlorella pseudocarpa* with a salinity of 0‰ to 10‰, i.e., desalinated *Chlorella pseudocarpa*. It should be noted that salinity refers to the proportion of dissolved solids (various salts) in water to the total mass of water; a salinity of 1‰ means that each liter of water contains 1g of dissolved salts.

[0068] In some examples, drying the desalinated *Micrococcus pluvialis* may include spray drying. Spray drying parameters include an inlet air temperature of 180–220°C and an outlet air temperature of 85–100°C. Spray drying yields dried algae with a water content of less than 5 wt%.

[0069] In some examples, step S20 may include enzymatic hydrolysis of defatted *Micrococcus pseudocarpa* with a specific enzyme at a second preset temperature for a second preset time.

[0070] In some examples, the second preset temperature in step S20 can be 50℃~60℃. For example, the preset temperature can be 50℃, 52℃, 54℃, 56℃, 58℃ or 60℃.

[0071] In some examples, in step S20, the specific enzyme may include at least one of alkaline protease and papain.

[0072] In some examples, in step S20, the second preset duration can be 5 to 10 hours. For example, the preset duration can be 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, or 10 hours.

[0073] In some examples, in step S20, the amount of enzyme used is 0.5% to 5% of the mass of the defatted *Micrococcus pluvialis*. For example, in step S20, the amount of enzyme used is 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5% of the mass of the defatted *Micrococcus pluvialis*. In some examples, in step S20, the amount of enzyme used is 1% to 3% of the mass of the defatted *Micrococcus pluvialis*.

[0074] In some examples, step S20 may include an enzyme inactivation process after the enzymatic hydrolysis. The enzyme inactivation process may include heating to a third preset temperature and maintaining this temperature for a third preset duration. The third preset temperature may be 90℃ to 98℃. The third preset duration may be 10 minutes to 20 minutes.

[0075] In some examples, step S20, after enzymatic hydrolysis, also includes precipitation. The reaction solution after enzymatic hydrolysis is allowed to stand and precipitate. The precipitation time can be 0.3 hours to 1 hour. The supernatant obtained after precipitation is used for subsequent operations.

[0076] In some examples, step S30, the purification process may include centrifugation, concentration, and drying. Centrifugation can remove insoluble algal residues. Concentration and drying can produce a powdered bioactive preparation.

[0077] In some examples, step S30 may include centrifuging the supernatant obtained after precipitation. Centrifugation parameters include: a rotation speed of 6000 rpm to 7000 rpm and a centrifugation time of 1 to 10 minutes. The supernatant may be retained after centrifugation to obtain the centrifuged supernatant.

[0078] In some examples, in step S30, concentration may include: heating the supernatant after centrifugation to a fourth preset temperature under negative pressure. The fourth preset temperature may be 65℃~75℃. After concentration, the volume of the supernatant after centrifugation may be reduced to 1 / 4~1 / 3. In some examples, in step S30, drying may include: spray drying the concentrated supernatant to obtain a bioactive formulation. The inlet air temperature of the spray may be 180℃~190℃, the atomizer frequency may be 170Hz~185Hz, and the outlet air temperature may be 85℃~95℃.

[0079] In some examples, step S30 may further include at least one of decolorization and deodorization. This reduces pigments such as chlorophyll and algal off-odors, improving the flavor and color of the bioactive preparation.

[0080] Figure 2 This is a flowchart illustrating a method for preparing a bioactive formulation according to another example of this disclosure.

[0081] like Figure 2 As shown, in some examples, the preparation method of the bioactive agent may further include a water extraction step (step 15). The water extraction step may be located between the defatting step and the enzymatic hydrolysis step. That is, in some examples, the preparation method of the bioactive agent may include: defatting (step S10), water extraction (step S15), enzymatic hydrolysis (step S20), and purification (step S30). Water extraction is beneficial for removing water-soluble impurities, including nucleic acids, polysaccharides, and inorganic salts.

[0082] In some examples, step S15 may include: taking defatted *Micrococcus pseudocarpa*, adding 8 to 12 times the volume of water, maintaining a fifth preset temperature for a fifth preset time, and continuously stirring; cooling to below 75°C and stopping stirring; allowing the mixture to stand and separate into layers, separating the supernatant and the bottom residue, wherein the bottom residue is the water-extracted *Micrococcus pseudocarpa*.

[0083] In some examples, the fifth preset temperature can be 90℃~95℃. For example, the fifth preset temperature can be 90℃, 91℃, 92℃, 93℃, 94℃, or 95℃. In this case, the high temperature can cause the cell wall to swell and the cell membrane to become more fluid. Combined with the mechanical force of stirring, this can facilitate the rapid dissolution of substances such as nucleic acids and polysaccharides. In addition, the high temperature can also improve the solubility of polysaccharides, nucleic acids, and salts. Specifically, the high temperature can reduce the hydrogen bonding between polysaccharide molecules, reduce polymerization and precipitation, and improve their solubility in water. Stirring can also prevent local aggregation of polysaccharides, forming a uniformly dispersed aqueous solution. The high temperature can cause the double strands of nucleic acids to unwind into single strands, and the single strands are more hydrophilic. The high temperature can further improve the dissolution rate of salts. At the same time, at the fifth preset temperature, the proteins in *Micrococcus pluvialis* will denature, destroying the secondary and tertiary structures of the proteins, but not the peptide bonds. The denatured protein molecules are easier to aggregate, which is beneficial for separation through subsequent processes and reduces the adsorption interference of proteins on nucleic acids and polysaccharides, thereby improving the purity of the target substances.

[0084] In some examples, the fifth preset duration can be 2 to 4 hours. For example, the fifth preset duration can be 2 hours, 2.5 hours, 3 hours, 3.5 hours, or 4 hours. This facilitates the full dissolution of water-soluble impurities such as nucleic acids, polysaccharides, and inorganic salts.

[0085] In some examples, the stirring rate can be from 10 rpm to 100 rpm. For example, the stirring rate can be 10 rpm, 20 rpm, 30 rpm, 40 rpm, 50 rpm, 60 rpm, 70 rpm, 80 rpm, 90 rpm, or 100 rpm. This facilitates the full dissolution of water-soluble impurities such as nucleic acids, polysaccharides, and inorganic salts.

[0086] In some examples, the hangover relief composition of this application can reduce the concentration of ethanol in human blood by more than 40%. In some examples, the hangover relief composition of this application can reduce the concentration of ethanol in human blood by 40% to 60%.

[0087] This embodiment also provides the application of a hangover relief composition or bioactive preparation in the preparation of hangover relief products.

[0088] In some examples, the dosage form of a hangover remedy can be selected from one of the following: lozenges, powders, capsules, tablets, suspensions, emulsions, solutions, and syrups. This allows for the selection of different dosage forms according to actual needs.

[0089] In some examples, hangover remedies may take the form of at least one of compressed candy, solid beverage, liquid beverage, and yogurt.

[0090] In some examples, hangover remedies promote the breakdown of ethanol within the digestive tract. For instance, hangover remedies can promote the breakdown of ethanol in the stomach and / or intestines.

[0091] In some examples, hangover remedies can be taken orally.

[0092] In some examples, hangover remedies can be taken before, during, or after drinking alcohol. In other examples, they can be taken 5 to 30 minutes before drinking. This allows the hangover remedy to first enter the gastrointestinal tract orally and then exert its effect there.

[0093] In some examples, the dosage of a hangover remedy product per use may be 0.1g to 1g of a bioactive agent. For example, the dosage may be 0.1g, 0.2g, 0.3g, 0.4g, 0.5g, 0.6g, 0.7g, 0.8g, 0.9g, or 1g of a bioactive agent. In other examples, the dosage may be 0.2g to 2g of a hangover-degrading enzyme preparation. For example, the dosage may be 0.2g, 0.4g, 0.6g, 0.8g, 1g, 1.2g, 1.4g, 1.6g, 1.8g, or 2g of a hangover-degrading enzyme preparation. This further helps to ensure a good hangover-reducing effect.

[0094] The following detailed explanation of the hangover relief composition, bioactive preparation, and application involved in this disclosure, in conjunction with the experimental process of specific embodiments, should not be construed as limiting the scope of protection of this disclosure. In this embodiment, unless otherwise specified, the materials, reagents, and instruments used are all commercially available, and the operating procedures are performed according to the instructions or general procedures of the reagents or instruments.

[0095] Example 1: I. Preparation of Bioactive Preparations (1) Preparation of defatted algae powder: Take the culture medium of *Nannochloropsis gaditana* (i.e., algal solution), and concentrate it by centrifugation to a dry weight of 5-15%. Repeat the washing and centrifugation of the concentrated algal solution until the salinity is 0-10‰, obtaining desalinated *Nannochloropsis gaditana*. Spray dry the desalinated *Nannochloropsis gaditana* at an inlet air temperature of 180-220℃ and an outlet air temperature of 85-100℃ to obtain dry algae with a final water content of less than 5wt%. Immerse the dry algae in 8 times its weight of ethanol at 75℃ for 75 minutes; the cell walls of *Nannochloropsis gaditana* rupture and release their contents (algal oil), thus obtaining the extract. Separate the extract from the microalgal residue; the resulting residue is a protein-rich microalgal meal, which, after drying, yields defatted algal powder.

[0096] (2) Water extraction treatment: Weigh 200 kg of defatted algae powder and add it to the reactor. Simultaneously, add 10 times the volume of room-temperature soft water to the reactor. Start stirring and heat to 90-95°C, maintain this temperature for 3 hours, then stop heating and begin cooling to 70°C. After cooling, stop stirring and allow the mixture to stand until it separates into layers. Collect the supernatant, centrifuge, concentrate, and spray-dry the supernatant to obtain the powdered product, which is the aqueous extract of *Micrococcus pseudocarpa*. After removing the supernatant, the remaining material proceeds to the next enzymatic hydrolysis step.

[0097] (3) Enzymatic hydrolysis: Turn on the cooling water in the jacket of the reactor to lower the temperature, and simultaneously add 10 times the volume of room temperature soft water into the reactor. Stop cooling after the material temperature reaches 50-55℃. Add saturated sodium hydroxide solution to adjust the pH of the material to 8.5-9.5. Add 1.5% of the feed amount of alkaline protease to the material to begin enzymatic hydrolysis. Turn on heating and maintain the temperature at 55±2℃ for 7 hours. Heat the solution to 95℃ and maintain for 15 minutes to inactivate the enzyme. After completion, continue stirring, turn on the cooling mode to lower the solution temperature to below 75℃, and then stop stirring to begin precipitation. After precipitation for 0.5 hours, extract the supernatant for centrifugation; stop when residue is seen. Add 5 times the volume of room temperature soft water for reconstitution, stir for 20 minutes, let stand for 30 minutes to precipitate, and then extract the supernatant for centrifugation.

[0098] (4) Separation and collection process steps (centrifugation, concentration, spraying, and collection): Centrifugation: Start the centrifuge feed. After the speed reaches a stable 6500 rpm, feed and circulate the material. Only after the outlet is visually clear and free of impurities can the transfer process begin. The supernatant obtained from centrifugation is concentrated using a single-effect evaporator.

[0099] Concentration: Turn on the single-effect evaporator and start the heating to maintain the temperature of the feed liquid at 70±5℃, continuously maintaining a vacuum. After concentrating the volume by 3-4 times, transfer the feed liquid to the spray drying process.

[0100] Spraying and collecting: After starting the spray, adjust the inlet air temperature to 183±3℃. Adjust the atomizer rate to 170~185Hz. After starting the feeding mode, adjust the feeding frequency to maintain the outlet air temperature at 90±1℃. Collect the powder every half hour using aluminum foil bags, and quickly exhaust and seal the bags after collection. After the batch production is completed, all products in this batch will be sieved (40 mesh screen). After sieving, they will be packaged in double-layer PE bags, 5Kg / bag. The bioactive preparation of Example 1 is obtained, which can also be called the pseudomicrococcus peptide powder of Example 1.

[0101] The above process was repeated for two batches. The first batch yielded 42 kg of *Pseudomonas aeruginosa* peptide powder, a pale yellow powder with no odor, and measured ash content of 6.3%, total protein content of 87.13%, total peptide content of 72.5%, polysaccharide content of 5.29%, and nucleic acid content of 1.40%. The second batch yielded 44 kg of *Pseudomonas aeruginosa* peptide powder, a pale yellow powder with no odor, and measured ash content of 6.76%, total protein content of 86.03%, total peptide content of 78.53%, and polysaccharide content of 5.54%.

[0102] (5) Amino acid composition detection For one batch of the bioactive preparation in Example 1, the amino acid composition was determined according to GB 5009.124-2016 "National Food Safety Standard - Determination of Amino Acids in Food" using an automated amino acid analyzer. The results are shown in Table 1 below: Table 1

[0103] II. Preparation of the hangover remedy composition The prepared bioactive preparation and compound enzyme powder were mixed at a mass ratio of 1:2, and excipients (microcrystalline cellulose filler) were added and compressed into tablets. Each tablet weighed 1g and contained 0.2g of the bioactive preparation, 0.4g of the compound enzyme powder, and 0.4g of excipients. The compound enzyme powder contained alcohol-degrading enzymes—ethanol dehydrogenase and aldehyde dehydrogenase—from *Acetobacter bassides*. The compound enzyme powder was purchased from Amecogen, product name: CureZyme-ACE Compound Fermented Vinegar Powder. Thus, the hangover-relieving composition of Example 1 was prepared.

[0104] Example 2: The bioactive preparation in Example 2 differs from that in Example 1 in that the step of "(2) water extraction" was omitted, and the defatted algae powder was directly subjected to enzymatic hydrolysis. The remaining steps are the same as in Example 1. Two batches of bioactive preparations were also prepared in Example 2. The first batch yielded 52.9 kg of *Pseudomonas aeruginosa* peptide powder, which was a light yellow powder with no odor. The ash content was measured to be 7.75%, the total protein content to be 81.3%, the total peptide content to be 69.1%, the polysaccharide content to be 7.71%, and the nucleic acid content to be 1.79%. The second batch yielded 54 kg of *Pseudomonas aeruginosa* peptide powder, which was a light yellow powder with no odor. The ash content was measured to be 9.06%, the total protein content to be 79.27%, the total peptide content to be 65.86%, and the polysaccharide content to be 8%.

[0105] The comparison shows that the bioactive formulation in Example 1 contains fewer impurities such as polysaccharides, salts, and nucleic acids, resulting in improved peptide powder product quality. Specific results are shown in Table 2 below. Compared to the bioactive formulation in Example 2, the bioactive formulation in Example 1 generally exhibits a 22.3% decrease in ash content, a 7.8% increase in total protein content, an 11.9% increase in total peptide content, a 31% decrease in polysaccharide content, and a 27.9% decrease in nucleic acid content.

[0106] Table 2

[0107] Example 2 Hangover Relief Composition: Similarly, the bioactive preparation and compound enzyme powder from Example 2 were mixed at a mass ratio of 1:2, and excipients (microcrystalline cellulose filler) were added, then compressed into tablets. Each tablet weighed 1g and contained 0.2g of the bioactive preparation, 0.4g of the compound enzyme powder, and 0.4g of the excipients. The compound enzyme powder contained alcohol dehydrogenase and acetaldehyde dehydrogenase from *Acetobacter bassii*. The compound enzyme powder was purchased from Amecogen, product name: CureZyme-ACE Compound Fermented Vinegar Powder. Thus, the hangover relief composition of Example 2 was prepared.

[0108] Experimental results on the efficacy of hangover remedies: (1) Alcohol-degrading enzymes + bioactive agents have a better hangover-degrading effect. In 50 mL of 20% ethanol solution, 0.6 g of compound enzyme powder (Aimeikejian, CureZyme-ACE compound fermented vinegar powder) was added as experimental group A; in 50 mL of 20% ethanol solution, 0.4 g of compound enzyme powder + 0.2 g of the bioactive preparation from Example 1 was added as experimental group B; in 50 mL of 20% ethanol solution, 0.4 g of compound enzyme powder + 0.2 g of the bioactive preparation from Example 2 was added as experimental group C. The mixture was stirred in a 37°C water bath, and the initial pH value was measured. Then, the pH value of the reaction system was measured every 30 minutes. Results are shown below. Figure 3 , Figure 4 and Figure 5 , Figure 3 This diagram shows the experimental results of the hangover-relieving effect of the compound enzyme powder involved in this disclosure. Figure 4 This is a graph showing the experimental results of the hangover relief effect of the hangover relief composition of Example 1, which is an example of this disclosure. Figure 5 This is a graph showing the experimental results of the hangover relief effect of the hangover relief composition of Example 2, which is an example of this disclosure. Figures 3 to 5 In the graph, the horizontal axis represents time (in hours), and the vertical axis represents pH value.

[0109] Figure 3During the process, since the compound enzyme powder itself is acidic, the pH decreases after the compound enzyme powder is added (0~1.5h); then the pH rises (1.5h~2.0h) because some ethanol is degraded into acetaldehyde by alcohol dehydrogenase; then the pH stabilizes at about 4.8 (2.0h~3.0h) because the acetaldehyde produced is degraded into acetic acid by acetaldehyde dehydrogenase, and the generation and degradation of acetaldehyde reach equilibrium. Figure 4 As can be seen, the addition of the compound enzyme powder plus the bioactive agent of Example 1 caused the pH to drop rapidly at first (0~0.5h); then the rate of pH decrease slowed down significantly (0.5h~1.0h), because alcohol dehydrogenase degraded ethanol into acetaldehyde, indicating that the bioactive agent can promote the activity of acetaldehyde dehydrogenase and make it take effect quickly; then the rate of pH decrease slightly increased (1.0h~1.5h), indicating that the acetaldehyde produced was further degraded into acetic acid by acetaldehyde dehydrogenase and the acetic acid production efficiency was greater than that of acetaldehyde. This shows that compared with the compound enzyme powder alone, the addition of the bioactive agent increased the activity of acetaldehyde dehydrogenase and increased the degradation of acetaldehyde and the production of acetic acid. The subsequent pH rise (1.5h~2.0h) indicates that when acetic acid is produced rapidly, the production of acetaldehyde may not keep up with its degradation. Therefore, acetaldehyde dehydrogenase, lacking substrate, reduces acetaldehyde degradation during this process. As acetaldehyde levels gradually increase, acetaldehyde dehydrogenase obtains sufficient substrate and begins to degrade acetaldehyde to produce acetic acid again at a rate greater than acetaldehyde production, causing the pH to drop again (2.0h~2.5h). Subsequently, equilibrium is reached, and the pH remains stable (2.5h~3.0h). It should be noted that both alcohol dehydrogenase and acetaldehyde dehydrogenase require NAD+ for their reactions. + As an enzyme donor, and with the rate of the two-step reaction depending on the concentration of the substrate and the activity of the enzyme, it is difficult to achieve equilibrium in the two-step polarized reaction. Figure 5 In the middle, trends and Figure 4 The results were similar, but the variation was smaller, indicating that the bioactive formulation in Example 2 was slightly less effective.

[0110] In summary, in the experimental group using the compound enzyme powder + bioactive preparation, the pH value decreased rapidly, followed by a rapid increase. This indicates a faster degradation of ethanol into acetaldehyde and further into acetic acid, reducing the residence time of acetaldehyde in the system and minimizing its harmful effects on the body. This combination demonstrates a better hangover-relieving effect than compound enzyme powder alone. This suggests that the combined use of compound enzyme powder and bioactive preparation increases the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase, resulting in a better hangover-relieving effect.

[0111] (2) Human testing of the hangover relief composition ① Ethanol Metabolism Rate Test: Participants were recruited and divided into three groups. One group took two tablets of the hangover relief composition of Example 1 (each tablet containing 0.4g of compound enzyme powder + 0.2g of bioactive agent) 15 minutes before drinking alcohol. Another group took two tablets of the hangover relief composition of Example 2 (each tablet containing 0.4g of compound enzyme powder + 0.2g of bioactive agent) 15 minutes before drinking alcohol. The last group did not take any hangover relief composition. Each participant drank the same amount of alcohol (3 liang of 42-degree baijiu). Blood samples were collected at 0, 15, 30, 60, 90, 120, 180, 270, 360, and 450 minutes to measure the concentration of ethanol in the blood. The average value was taken, and the blood ethanol concentration at each time point was calculated for each group of participants. Figure 6 This is a graph showing the results of ethanol metabolism rate tests for different products involved in the examples of this disclosure. Figure 6 In the graph, the horizontal axis represents time (in minutes), and the vertical axis represents the blood ethanol content (in mg / 100mL). The top curve represents the ethanol metabolism rate test results for the group that did not take the hangover relief composition, the middle curve represents the ethanol metabolism rate test results for the group that took the hangover relief composition of Example 2, and the bottom curve represents the ethanol metabolism rate test results for the group that took the hangover relief composition of Example 1. Figure 6 As shown, the blood ethanol concentration of test subjects using the hangover relief composition of Example 1 was significantly lower than that of test subjects who did not use the composition, and it began to take effect within 15 minutes. The blood ethanol concentration reached its peak near 30 minutes and began to decline, while the blood ethanol concentration of test subjects who did not use the composition did not reach its peak until 60 minutes, and the peak value was more than twice that of the peak value. The blood ethanol concentration of test subjects using the hangover relief composition of Example 1 decreased rapidly within 180 minutes, and by 180 minutes, the blood ethanol concentration had decreased by approximately 90% from the peak value, while the blood ethanol concentration of test subjects who did not use the composition did not decrease to that value until 360 minutes. The hangover relief composition of Example 2 was slightly less effective than that of Example 1. In summary, the hangover relief compositions of Example 1 or Example 2 can significantly reduce the amount of ethanol entering the bloodstream and accelerate ethanol metabolism, causing the blood ethanol concentration to reach its peak value more quickly and then decline rapidly, thereby effectively shortening the retention time of ethanol in the body.

[0112] ② Subjective Experience Test: Participants were recruited to test various types of alcohol. They took two tablets of the hangover remedy composition from Example 1 (each tablet containing 0.4g of compound enzyme powder + 0.2g of bioactive agent) 15 minutes before drinking the alcohol and underwent a subjective experience test. Participants scored the product on a scale of 10, from ineffective to effective, based on the degree of relief of post-drinking discomfort (weight 40%), the speed of recovery to sobriety (weight 30%), and the level of subjective satisfaction with the effect (weight 30%). A final score higher than 6 points was considered effective. Figure 7This diagram illustrates the results of a test on the perceived effectiveness of the hangover remedy composition of Example 1, which is an example of this disclosure. The results show that the effectiveness rate reached 100% in the group that drank baijiu (5 effective participants); 100% in the group that drank red wine (9 effective participants); 75% in the group that drank beer (17 effective participants); 100% in the group that drank Moutai (2 effective participants); and 80% in the group that drank Shaoxing rice wine (12 effective participants). In summary, the hangover remedy composition is significantly effective for most types of alcohol, especially baijiu, red wine, and Moutai, achieving a 100% effectiveness rate.

[0113] ③ Breathalyzer Test: Each person ingested 200mL of 53% ABV liquor in a single serving. Fifteen minutes prior to ingestion, they took two tablets of the hangover remedy composition described in Example 1 (each tablet containing 0.4g of compound enzyme powder + 0.2g of bioactive agent), and then a breathalyzer test was conducted to determine the alcohol concentration. One of the breathalyzer test results is shown below. Figure 8 , Figure 8 This diagram illustrates the results of a breathalyzer test after using the hangover relief composition of Example 1, as described in the present disclosure. The results show that after using the hangover relief composition of Example 1, the amount of ethanol exhaled from the mouth decreased significantly over time, with rapid detoxification within 15 minutes, and the concentration of ethanol in the mouth decreasing to half; after 30 minutes, it decreased to below 70 mg / 100 mL.

[0114] While the present disclosure has been specifically described above in conjunction with the accompanying drawings and examples, it is to be understood that the foregoing description does not limit the present disclosure in any way. Those skilled in the art can make modifications and variations to the present disclosure as needed without departing from its essential spirit and scope, and all such modifications and variations shall fall within the scope of the present disclosure.

Claims

1. A hangover remedy composition, characterized in that, It includes alcohol-degrading enzyme preparations and bioactive preparations. The alcohol-degrading enzyme preparations include at least one of alcohol dehydrogenase and acetaldehyde dehydrogenase. The bioactive preparations include products obtained by enzymatic hydrolysis of proteins contained in Micrococcus pseudocarpa, and the products include polypeptide substances.

2. The hangover relief composition according to claim 1, characterized in that, The alcohol-degrading enzyme preparation includes alcohol dehydrogenase and acetaldehyde dehydrogenase.

3. The hangover relief composition according to claim 1 or 2, characterized in that, The alcohol-detoxifying enzyme preparation is fermented vinegar powder, and the mass ratio of the fermented vinegar powder to the bioactive preparation in the alcohol-detoxifying composition is 1:1 to 3:

1.

4. The hangover relief composition according to claim 3, characterized in that, The fermented vinegar powder is a fermentation product produced by microorganisms with ethanol metabolism capabilities using ethanol as a substrate. The microorganisms contain alcohol dehydrogenase and acetaldehyde dehydrogenase.

5. The hangover relief composition according to claim 4, characterized in that, In the fermented vinegar powder, the ratio of enzyme activity units of alcohol dehydrogenase to enzyme activity units of acetaldehyde dehydrogenase is 1:1 to 1:

3.

6. The hangover relief composition according to claim 4, characterized in that, The microorganisms include at least one of Pasteurella acetic acid bacteria, lactic acid bacteria and Bacillus subtilis, wherein the lactic acid bacteria include at least one of Lactobacillus helveticus, Lactobacillus plantarum and Streptococcus thermophilus.

7. The hangover relief composition according to claim 1, characterized in that, The enzymes used in the enzymatic hydrolysis include at least one of human or animal proteases, plant proteases, and microbial proteases. The human or animal proteases include at least one of salivary protease, trypsin, pepsin, and chymotrypsin. The plant proteases include at least one of papain and bromelain. The microbial proteases include at least one of subtilisin and Aspergillus protease.

8. The hangover relief composition according to claim 1, characterized in that, The total peptide content in the bioactive preparation exceeds 60%.

9. The hangover relief composition according to claim 1, characterized in that, The hangover relief composition meets at least one of the following conditions: The hangover relief composition promotes the breakdown of ethanol in the digestive tract, blood, or tissues; The hangover relief composition is taken orally 5 to 30 minutes before or after drinking alcohol. The dosage of the hangover relief composition per use is: 0.1g to 1g of the bioactive preparation; The hangover relief composition may be in the form of at least one of compressed candy, solid beverage, liquid beverage, and yogurt.

10. The use of a hangover relief composition as described in any one of claims 1 to 9 in the relief of hangovers and / or the preparation of hangover relief products.

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