A method for detecting misoprostol acid concentration in blood by liquid chromatography tandem mass spectrometry

Abstract

The application belongs to the technical field of pharmaceutical analysis, and particularly relates to a liquid chromatography tandem mass spectrometry detection method for misoprostol acid concentration in blood. The pretreatment method of the application does not need any special solid phase extraction (SPE) equipment or consumables, and can be completed only by using a conventional pipette and a centrifuge, greatly simplifying the operation, shortening the pretreatment time, and significantly reducing the single sample analysis cost. In addition, compared with the existing liquid-liquid extraction (LLE) method, the minimum quantification limit (LLOQ) is pushed from 10 pg / mL to 2.5 pg / mL, and the sensitivity is increased by 4 times, which makes the method of the application meet the demand of blood drug concentration monitoring of misoprostol acid in the low-dose oral scheme in the clinic. The formic acid-tert-butyl methyl ether LLE extraction system is selected, and the use of dichloromethane with high toxicity is avoided. Therefore, the detection method of the application has important clinical significance and broad market application prospect.

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