Dosing method for degrading quinclorac residues in tobacco and rice rotation field
By using potassium persulfate for chemical oxidation and degradation in tobacco seedling transplanting holes, the problem of remediation of dichloroquinoline acid residues in tobacco-rice rotation fields was solved, achieving rapid, economical, and environmentally friendly soil remediation effects, and avoiding the shortcomings of traditional methods.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- FUJIAN TOBACCO
- Filing Date
- 2026-03-16
- Publication Date
- 2026-04-21
AI Technical Summary
Existing technologies are insufficient to effectively, quickly, and economically remediate dichloroquinoline residues in rice-tobacco rotation fields, leading to soil pollution and tobacco pesticide damage. Furthermore, traditional methods may cause secondary pollution or disrupt the ecological balance.
Potassium persulfate was used as the root-setting water to degrade the residual dichloroquinoline acid in the transplanting hole and surrounding soil of tobacco seedlings through in-situ chemical oxidation. The chemical oxidation remediation was achieved by using a mass ratio of potassium persulfate to dichloroquinoline acid of (400~4000):1, a concentration of 0.01%, and a dosage of 500 mL/plant.
It significantly degrades dichloroquinoline acid, restores soil ecological balance, avoids the need for whole-field application to reduce costs, provides rapid and effective soil remediation, prevents tobacco pesticide damage, and has universal applicability and environmental friendliness.
Smart Images
Figure CN121892497A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of soil remediation technology, and more specifically to an administration method for degrading dichloroquinoline acid residues in rice-tobacco rotation fields. Background Technology
[0002] Tobacco-rice rotation is an agricultural farming model that alternates the planting of flue-cured tobacco and rice within a year or season. Through "water-dry rotation", it achieves efficient land use, reduced pests and diseases, improved soil fertility, and increased farmers' income. It can effectively improve the soil ecology, enhance the quality and efficiency of tobacco and grain production, and promote agricultural efficiency and farmers' income.
[0003] In rice-tobacco rotation areas, due to labor shortages in rural areas, herbicides, with their advantages of high efficiency, low cost, and convenience, are playing an increasingly important role in weed management in paddy fields. However, with the increasing scope and amount of herbicide use, the pollution of surface and groundwater caused by herbicides is becoming increasingly serious, which has attracted widespread attention. Besides polluting water bodies through migration, herbicides can also easily cause direct or indirect phytotoxicity to crops. For tobacco, a crop that relies on vegetative organs for harvesting, the impact is even more significant.
[0004] Currently, soil remediation technologies for herbicide residues are mainly divided into physical remediation, bioremediation, and chemical remediation. Among them, physical remediation mainly involves methods such as soil turning and replacement. Although it can temporarily reduce the concentration of herbicides in the soil of a specific area, its essence is the transfer of pollutants rather than their elimination. This method not only fails to fundamentally reduce the total amount of pollution, but may also lead to the spread of pollutants during the operation, causing the risk of secondary pollution and resulting in poor environmental sustainability.
[0005] Bioremediation utilizes microorganisms and plants to absorb, degrade, and transform pollutants in soil, and is considered an environmentally friendly approach. However, the remediation effectiveness of this technology is highly dependent on a variety of complex and difficult-to-control external factors, such as climate and geographical conditions, the chemical structure and form of pollutants (e.g., quinclorac), and the degree of soil pollution. The uncontrollability of these factors leads to unstable efficiency and potentially long cycles in the bioremediation process, making it difficult to meet the needs of intensive agricultural production such as tobacco-rice rotation for rapid and reliable remediation.
[0006] Chemical remediation involves applying chemical amendments to the soil. This method alters the internal environment of herbicide-contaminated soil, accelerating the degradation of herbicide residues. It offers a direct, rapid, and highly effective remediation of herbicide-induced plant damage. When remediating soil contaminated with herbicides (organic pollution), it is particularly effective in removing organic pollutants. Furthermore, these surfactants are more easily degraded, offering advantages such as direct action, rapid results, and significant removal of organic pollutants, thus demonstrating a promising future. It is worth noting that while using chemical reagents to alter the physicochemical properties of herbicide-contaminated soil is a direct and rapid solution, improper handling (such as unscientific reagent selection, dosage, or application methods) can introduce chemicals that may disrupt the soil's ecological balance or introduce new environmental pollution. Therefore, maximizing the high efficiency of chemical remediation while minimizing its potential negative effects is a key challenge in its application.
[0007] Therefore, developing novel chemical remediation technologies to efficiently degrade herbicide residues is one of the important research topics in the current management of herbicide residue damage in tobacco-rice rotation fields. Summary of the Invention
[0008] In view of this, the purpose of the present invention is to provide an administration method for degrading dichloroquinoline acid residues in rice-tobacco rotation fields, so as to overcome the shortcomings of the prior art.
[0009] To achieve the above objectives, the present invention adopts the following technical solution:
[0010] A method for administering medication to degrade dichloroquinoline acid residues in tobacco-rice rotation fields, specifically including the following steps: (1) Make holes in the tobacco ridges and transplant the tobacco seedlings into the holes for planting; (2) On the day of or the day after the tobacco seedlings are transplanted, potassium persulfate is used as the root-setting water for irrigation. The residual dichloroquinoline acid in the hole and surrounding soil is oxidized and degraded through in-situ chemical oxidation.
[0011] Quinclorac is a hormone-type herbicide; even extremely low residues can cause deformities and even crop failure in tobacco plants. In hydroponic experiments, the inventors found that at a concentration of 0.025 mg / L quinclorac, the herbicide effects on tobacco seedlings were already quite evident, with new leaves showing obvious curling. At this point, the addition of 0.01% potassium persulfate resulted in better seedling development, with no deformities in the new leaves, demonstrating that potassium persulfate oxidized and degraded quinclorac. However, the addition of lower concentrations of potassium persulfate (0.001% and 0.0001%) caused varying degrees of herbicide damage in the seedlings. Based on this, this invention changes the traditional control method, providing a method for preventing herbicide damage. This method involves applying potassium persulfate directly to the transplanting hole, using in-situ chemical oxidation to degrade quinclorac residues in the soil, creating a clean soil environment free of organic pollutants. This purifies the quinclorac residues, eradicates the herbicide-induced damage to tobacco, and thus achieves the effect of relieving tobacco herbicide damage and restoring the soil.
[0012] Furthermore, in step (2) above, the mass ratio of potassium persulfate to dichloroquinoline acid is (400~4000):1.
[0013] The further beneficial effect of the above-mentioned method is that the present invention applies the oxidant potassium persulfate to the soil contaminated with dichloroquinoline acid to ensure that the agent can fully contact the pollutants, thereby achieving an effective remediation effect.
[0014] Furthermore, in step (2) above, the concentration of potassium persulfate as the root-setting water is 0.01%.
[0015] Furthermore, in step (2) above, the amount of potassium persulfate used as root-setting water is 500 mL / plant.
[0016] As can be seen from the above technical solution, compared with the prior art, the beneficial effects of the present invention are as follows: (1) Using in-situ chemical oxidation technology to remediate herbicide residues in tobacco-rice rotation areas Tobacco is highly sensitive to quinclorac residues in soil, and herbicide damage to tobacco leaves is severe in tobacco-rice rotation areas. In-situ chemical oxidation remediation is a widely applicable technique for remediation under subsurface conditions, but its application in remediating herbicide residues in soil has not been reported. This invention targets quinclorac, a commonly used tobacco herbicide, and develops a new in-situ chemical oxidation remediation technology for quinclorac residues in soil. This technology can also be extended to other common tobacco herbicides such as acetochlor, butachlor, and bensulfuron-methyl.
[0017] (2) The research on the in-situ chemical oxidation method for remediating herbicide residues in tobacco fields has universal significance. This invention not only provides a theoretical basis for mitigating quinclorac phytotoxicity, but also offers theoretical and technical support for achieving integrated green pest control in agricultural practices and environmental protection. More importantly, this invention has significant exemplary value, serving as a model for the remediation of other types of herbicide pollution.
[0018] (3) Simple and effective Chemical oxidation repair using specific agents is simple to operate and has obvious effects.
[0019] (4) Good economic efficiency The agent has a relatively low cost, significant remediation effect, and good economic efficiency. Using the method of applying the agent within the transplanting hole restores the soil environment within and around the hole, maximizing the benefits of localized remediation, avoiding application across the entire field, and significantly reducing usage costs.
[0020] (5) Restoration of soil ecological balance and health Chemical oxidative remediation can alter the chemical properties of the soil, accelerate the degradation of herbicide quinclorac residues, and restore the ecological balance and health of the soil.
[0021] (6) Source control approach This approach changes the traditional method of controlling herbicide residues by using fallow land and activated carbon adsorption. Instead, it uses intra-planting herbicide application to achieve source control of herbicide residues in tobacco fields, providing a theoretical basis and technical support for precise prevention and control of herbicide residues in tobacco fields. Attached Figure Description
[0022] Figure 1 The standard curve for dichloroquinoline acid solution; Figure 2 A kinetic model for the degradation of the herbicide dichloroquinoline acid by the oxidant potassium persulfate. Detailed Implementation
[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0024] Example 1 The method of administration for degrading dichloroquinoline acid residues in tobacco-rice rotation fields specifically includes the following steps: (1) Make holes in the tobacco ridges and transplant the tobacco seedlings into the holes for planting; (2) On the day of transplanting the tobacco seedlings, 0.01% potassium persulfate was used as the root-setting water, and 500 mL was applied per plant for root irrigation. The residual dichloroquinoline acid in the hole and surrounding soil was oxidized and degraded through in-situ chemical oxidation.
[0025] Example 2 The method of administration for degrading dichloroquinoline acid residues in tobacco-rice rotation fields specifically includes the following steps: (1) Make holes in the tobacco ridges and transplant the tobacco seedlings into the holes for planting; (2) On the day after the tobacco seedlings are transplanted, 0.01% potassium persulfate is used as the root-setting water, and 500 mL is applied per plant for root irrigation. The residual dichloroquinoline acid in the hole and surrounding soil is oxidized and degraded through in-situ chemical oxidation.
[0026] Performance testing 1. Plotting the standard curve Existing literature indicates that the harmful concentration of dichloroquinoline acid in tobacco is 0.1 mg / kg. Typical symptoms of phytotoxicity include leaf tip and margin curling towards the back of the leaf, thickening and narrowing of the leaves, and in severe cases, linear leaf shapes. Furthermore, phytotoxicity significantly reduces tobacco leaf quality, decreasing total sugar, reducing sugar, and potassium content, while significantly increasing nicotine and starch content. Therefore, a series of dichloroquinoline acid standard working solutions with concentrations of 0.025, 0.05, 0.1, 0.2, 0.3, 0.4, 0.6, and 0.8 mg / L were prepared. The standard curve was obtained by measuring the peak area of each standard solution (Table 1): y = 238.61x + 3.2212 (R²). 2 =0.996)( Figure 1 ).
[0027] Table 1. Plotting the standard curve of dichloroquinoline acid solution
[0028] 2. Degradation kinetic model Based on the harmful concentration of quinclorac acid in tobacco, its lowest detectable concentration in HPLC, and previous experiments showing that tobacco cannot grow normally in soil containing more than 0.01% potassium persulfate, an initial concentration of the herbicide quinclorac acid and the oxidant potassium persulfate was designed at 0.3 mg / L and 0.1% (1000 mg / L) (10 times the actual dose), respectively. A degradation kinetic model of quinclorac acid in potassium persulfate solution was analyzed. Specifically, a 25 mL mixed reaction solution containing 0.3 mg / L quinclorac acid and 0.1% potassium persulfate was prepared, and the residual amount of quinclorac acid was measured at time intervals, with three biological replicates. The kinetic model of the degradation of the herbicide quinclorac acid by potassium persulfate was finally obtained.
[0029] According to the model, ( Figure 2After reacting 0.3 mg / L dichloroquinoline acid with 0.1% potassium persulfate for 48 hours, almost no dichloroquinoline acid residue remained. Meanwhile, the control solution (0.3 mg / L dichloroquinoline acid solution) without added oxidant showed no significant change in dichloroquinoline acid content after 48 hours, indicating that potassium persulfate has a good degradation effect on dichloroquinoline acid.
[0030] 3. Degradation effect test To verify the degradation effect of potassium persulfate on dichloroquinoline acid, hydroponic and soil cultivation experiments were conducted using Cuibi No. 1, the main tobacco variety cultivated in Fujian, as the research object.
[0031] In the hydroponic experiment, appropriate amounts of quinclorac acid and potassium persulfate were added to Hoagland broth to prepare solutions with gradient ratios. After the prepared solutions were left at room temperature for 24 hours, tobacco seedlings were planted, with three seedlings planted for each treatment. After 12 days of planting (as shown in Table 2): In the presence of 0.025 mg / L quinclorac acid, the herbicide effects on the seedlings were already very obvious (the seedlings in hydroponics were more sensitive to pesticides), with obvious curling of new leaves. Meanwhile, in the case of 0.025 mg / L quinclorac acid solution with the addition of 0.01% potassium persulfate, the seedlings developed better, and no deformities were observed in the new leaves, demonstrating that potassium persulfate oxidized and degraded quinclorac acid. However, when lower concentrations of potassium persulfate (0.001% and 0.0001%) were added to the 0.025 mg / L quinclorac acid solution, the seedlings showed varying degrees of herbicide damage.
[0032] Table 2. Hydroponic experiment on the degradation of the herbicide dichloroquinoline by potassium persulfate.
[0033] In the soil culture experiment, soil containing 0.04 mg / kg quinclorac was prepared, and a corresponding potassium persulfate solution was added. The soil was soaked in water and mixed thoroughly. After standing overnight, tobacco seedlings were planted, and their growth was observed regularly. The results showed (as shown in Table 3) that, compared with the hydroponic experiment, the soil-cultured tobacco seedlings took longer to show symptoms, with obvious phenotypes appearing approximately 30 days after planting. Compared with the blank control (CK), under the condition of 0.04 mg / kg quinclorac, the growth of tobacco seedlings without added oxidant was affected, and the seedlings were severely deformed, with new leaves curling inward severely, giving the leaves a linear shape. However, when 0.01% potassium persulfate was added, the phytotoxicity of quinclorac to the tobacco seedlings was significantly alleviated, and the new leaves curled inward slightly without affecting the overall leaf morphology. In addition, when an appropriate amount of potassium persulfate (0.01%) was added, the tobacco seedlings grew normally. Therefore, when the mass ratio of potassium persulfate to quinclorac acid is (400~4000):1, the quinclorac acid in the soil can be greatly reduced, the herbicide is degraded well, and the tobacco seedlings grow normally.
[0034] Table 3. Soil culture experiment on the degradation of the herbicide dichloroquinoline by potassium persulfate.
[0035] The above experiments demonstrate that potassium persulfate has a good degradation effect on dichloroquinoline acid and has the potential to remediate pesticide-contaminated soil, providing an important reference for further research and application of chemical oxidation remediation of pesticide-contaminated soil.
[0036] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A method for administering medication to degrade dichloroquinoline acid residues in tobacco-rice rotation fields, characterized in that, Specifically, the following steps are included: (1) Make holes in the tobacco ridges and transplant the tobacco seedlings into the holes for planting; (2) On the day of or the day after the tobacco seedlings are transplanted, potassium persulfate is used as the root-setting water for irrigation. The residual dichloroquinoline acid in the hole and surrounding soil is oxidized and degraded through in-situ chemical oxidation.
2. The method for administering medication to degrade dichloroquinoline acid residues in rice-tobacco rotation fields according to claim 1, characterized in that, In step (2), the mass ratio of potassium persulfate to dichloroquinoline acid is (400~4000):
1.
3. The method for administering medication to degrade dichloroquinoline acid residues in tobacco-rice rotation fields according to claim 1, characterized in that, In step (2), the concentration of potassium persulfate as the root-setting water is 0.01%.
4. The method for administering medication to degrade dichloroquinoline acid residues in tobacco-rice rotation fields according to claim 3, characterized in that, In step (2), the amount of potassium persulfate used as root-setting water is 500 mL / plant.