Preparation method of polycarbonate plastic glass slide

Abstract

The invention discloses a preparation method of a polycarbonate plastic glass slide, which comprises the following preparation steps: S1, selecting polycarbonate glass slides with different sizes and specifications, soaking the polycarbonate glass slides in deionized water before an experiment, cleaning the polycarbonate glass slides for a certain time by an ultrasonic cleaner, and wiping off water stains on the cleaned polycarbonate glass slides by using anti-static dust-free cloth; s2, the polycarbonate glass slide is placed in a constant-temperature drying oven to be dried for a certain time at the constant temperature and then taken out. The polycarbonate plastic glass slide produced by adopting the preparation method has the characteristics of being colorless, transparent, low in cost, simple in production process method, high in production efficiency and the like, and meanwhile, the surface hydrophilic performance of the produced polycarbonate plastic glass slide is controllable; the plastic glass slide effectively solves the problems that the cover glass is broken or the tissue specimen is scratched and falls off due to strong adhesive force between the glass glass slide and the aqueous sample, and the plastic glass slide has the advantages of being simple to recycle and dispose after being used, small in environmental pollution, not easy to damage and the like.

Description

Technical Field

[0001] This invention belongs to the field of glass slide preparation technology, specifically a method for preparing polycarbonate plastic glass slides. Background Technology

[0002] A glass slide is a glass or quartz slide used to hold specimens when observing them under a microscope. During sample preparation, cell or tissue sections are placed on the slide, and a coverslip is placed on top for observation. Currently, glass slides are primarily made of glass. After use, glass slides are classified as hazardous medical waste and require separate recycling and disposal, making them difficult to dispose of collectively with other plastic medical waste. The post-use disposal of glass slides involves several complex processes, including biological disinfection and chemical treatment. The management procedures and disposal standards at each stage are extremely stringent. Glass slides cannot be reused and must be disposed of as medical waste, resulting in high disposal costs and significant environmental harm. This not only pollutes the environment but also causes serious waste of resources. Furthermore, glass slides are highly hydrophilic, creating strong adhesion between them and the sample being examined. Especially after the coverslip is placed on, the presence of liquid sample on the contact surface leads to strong adhesion, making it difficult to remove and prone to breakage or scratching and detachment of the tissue specimen, directly affecting the observation results. Researchers have considered using transparent plastic as a slide material to address the problems of difficult disposal, high cost, and significant environmental pollution associated with glass slides after use, and have conducted extensive research on the use of plastic slides. Studies have found that polycarbonate plastic has low surface energy, high inertness, and poor hydrophilicity, resulting in weak interfacial adhesion between plastic slides and aqueous samples, making it difficult to wet and adhere to aqueous samples. If polycarbonate plastic is used directly as a slide for aqueous samples, the poor interfacial adhesion leads to poor observation results or even renders the samples unusable, greatly limiting the use of plastic slides. To date, commercially available slides are primarily made of glass, and plastic slides, especially polycarbonate, are not commonly found. Therefore, we propose a method for preparing polycarbonate plastic slides. Summary of the Invention

[0003] To address the shortcomings of existing technologies, this invention provides a method for preparing polycarbonate plastic glass slides to solve the above-mentioned technical problems.

[0004] To achieve the above objectives, the present invention provides the following technical solution: a method for preparing a polycarbonate plastic glass slide, the preparation steps of which include: S1. Select polycarbonate slides of different sizes and specifications. Before the experiment, soak the polycarbonate slides in deionized water and clean them with an ultrasonic cleaner for a certain period of time. Wipe the cleaned polycarbonate slides with an anti-static lint-free cloth to remove water stains. S2. After drying the polycarbonate slides in a constant temperature oven for a certain period of time, remove them and pick out any unqualified slides with rough surfaces, cracks, damage, or air bubbles. Qualified slides are ready for use. S3. Select self-made positive molds of different shapes and place them on the hot press. Place the dried, flat and transparent polycarbonate glass slide between the positive mold and the upper platen of the hot press. Put in the hot press device and heating module. When the temperature of the hot press is set to the glass transition temperature, pressurize until the pressure rises to the set value and is maintained for a certain period of time. Then, cool the upper and lower heating blocks to near room temperature by passing tap water through the cooling block. Lift the hot press and demold to obtain a polycarbonate substrate with different microchannel structures. S4. Place the polycarbonate substrate under an irradiation source to irradiate the polycarbonate substrate, and process the sample by controlling the energy density and irradiation time; S5. Drop water onto the surface of the treated polycarbonate substrate, observe the morphology of the water droplets, and use a water contact angle tester to test the hydrophilic properties of the polycarbonate glass slide surface to achieve performance testing and evaluation.

[0005] Preferably, in step S1, the thickness of the polycarbonate slide is between 0.5 and 1.5 mm, and the cleaning time is 5 to 10 minutes.

[0006] Prior to step S1, the antistatic cleanroom cloth is made of double-woven polyester fiber. The wiping method is to wipe it up and down 3 times and left and right 3 times. Observe whether there are water stains on the outer surface of the polycarbonate slide. If water stains still exist, continue wiping.

[0007] Preferably, the temperature for constant temperature drying in step S2 is 105-130℃, and the drying time is 4-24 hours.

[0008] In step S2, defective slides are picked out by visual inspection.

[0009] Preferably, in step S3, the hot press is a constant temperature hot press, the number of hot press plates is 6 sets, and the spacing between the hot press plates is 100mm.

[0010] Preferably, in step S3, the set temperature of the hot press is 148-151℃, the set pressure of the hot press is 0.5-1.5MPa, and the set pressure holding time of the hot press is 2-10min.

[0011] Preferably, in step S4, the irradiation source is plasma and X-ray, the plasma power is 100-1000W, the irradiation time is 1-10 min, the absorbed dose of X-rays is 10-1000 Gy, and the dose rate is 1-10 Gyh. −1 .

[0012] Prior to this, the steps for using the water contact angle tester in step S5 are as follows: A1. Place a drop of the liquid to be tested onto a glass slide using an injection needle; A2. Measure hydrophilic properties with a single button press.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows: The polycarbonate plastic slides produced by this invention are colorless and transparent, low in cost, simple in production process, and high in production efficiency. Simultaneously, the surface hydrophilicity of the produced polycarbonate plastic slides is controllable, effectively solving problems such as coverslip breakage or tissue specimen scratches and detachment caused by strong adhesion between glass slides and aqueous samples. These plastic slides also offer advantages such as simple post-use recycling and disposal, minimal environmental pollution, and resistance to damage. Detailed Implementation

[0014] Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example 1

[0015] A method for preparing a polycarbonate plastic glass slide, comprising the following steps: S1. Select a polycarbonate glass slide with dimensions of 25.0mm×75.0mm×0.5mm, immerse it in deionized water, and clean it with an ultrasonic cleaner for 10 minutes. Wipe the cleaned polycarbonate glass slide with an antistatic lint-free cloth to remove water stains. S2. After drying the polycarbonate slides in a constant temperature oven at 105°C for 24 hours, remove them and pick out any unqualified slides with rough surfaces, cracks, damage, or air bubbles. Qualified slides are ready for use. S3. Select a male mold with an upper bottom width of 120μm, a lower bottom width of 80μm, and a depth of 50μm and place it on a hot press. Place a dried, flat, and transparent polycarbonate glass slide between the male mold and the upper platen of the hot press. Put in the hot press device and heating module, set the temperature of the hot press to 150°C and maintain this temperature stably. Press the hot press to a pressure of 0.5MPa and maintain the pressure at 0.5MPa for 10 minutes. After the specified time, cool the upper and lower heating blocks to near room temperature through tap water in the cooling block. Lift the hot press and demold to form a polycarbonate substrate with an average depth of 50μm microchannel structure. S4. Place it under plasma to irradiate the polycarbonate substrate. Adjust the heating power to 200W and the irradiation time to 5 minutes to complete the processing of the polycarbonate glass slide. S5. Drop water onto the surface of the treated polycarbonate substrate, observe the morphology of the water droplets, and use a water contact angle tester to test the hydrophilic properties of the polycarbonate glass slide surface to achieve performance testing and evaluation. Example 2

[0016] A method for preparing a polycarbonate plastic glass slide, comprising the following steps: S1. Select a polycarbonate glass slide with dimensions of 25.0mm×75.0mm×1.5mm, immerse it in deionized water, and clean it with an ultrasonic cleaner for 5 minutes. Wipe the cleaned polycarbonate glass slide with an antistatic lint-free cloth to remove water stains. S2. Place the polycarbonate slides in a 130°C constant temperature oven and dry them for 6 hours. Remove the slides and pick out any unqualified slides with rough surfaces, cracks, damage, or air bubbles. Set aside the qualified slides for use. S3. Select a male mold with an upper bottom width of 120μm, a lower bottom width of 80μm, and a depth of 50μm and place it on a hot press. Place a dried, flat, and transparent polycarbonate glass slide between the male mold and the upper platen of the hot press. Put in the hot press device and heating module, set the temperature of the hot press to 150°C and maintain this temperature stably. Press the hot press to a pressure of 0.5MPa and maintain the pressure at 1.0MPa for 10 minutes. After the specified time, cool the upper and lower heating blocks to near room temperature through the tap water in the cooling block. Lift the hot press and demold to form a polycarbonate substrate with an average depth of 50μm microchannel structure. S4. Place it under plasma to irradiate the polycarbonate substrate. Adjust the heating power to 100W and the irradiation time to 10min to complete the processing of the polycarbonate glass slide. S5. Drop water onto the surface of the treated polycarbonate substrate, observe the morphology of the water droplets, and use a water contact angle tester to test the hydrophilic properties of the polycarbonate glass slide surface to achieve performance testing and evaluation. Example 3

[0017] A method for preparing a polycarbonate plastic glass slide, comprising the following steps: S1. Select a polycarbonate glass slide with dimensions of 25.0mm×75.0mm×1.2mm, immerse it in deionized water, and clean it with an ultrasonic cleaner for 5 minutes. Wipe the cleaned polycarbonate glass slide with an antistatic lint-free cloth to remove water stains. S2. Place the polycarbonate slides in a 120°C constant temperature oven and dry them for 6 hours. Remove the slides and pick out any unqualified slides that have rough surfaces, cracks, damage, or air bubbles. Set aside the qualified slides. S3. Select a male mold with an upper bottom width of 120μm, a lower bottom width of 80μm, and a depth of 50μm and place it on a hot press. Place a dried, flat, and transparent polycarbonate glass slide between the male mold and the upper platen of the hot press. Put in the hot press device and heating module, set the temperature of the hot press to 150°C and maintain this temperature stably. Press the hot press to a pressure of 0.5MPa and maintain the pressure at 1.5MPa for 8 minutes. After the specified time, cool the upper and lower heating blocks to near room temperature through tap water in the cooling block. Lift the hot press and demold to form a polycarbonate substrate with an average depth of 50μm microchannel structure. S4. Irradiate the polycarbonate substrate under X-rays with an absorbed dose of 100 Gy and an absorbed dose rate of 5 Gyh. −1 To complete the processing of the polycarbonate glass slide; S5. Drop water onto the surface of the treated polycarbonate substrate, observe the morphology of the water droplets, and use a water contact angle tester to test the hydrophilic properties of the polycarbonate glass slide surface to achieve performance testing and evaluation. Example 4

[0018] A method for preparing a polycarbonate plastic glass slide, comprising the following steps: S1. Select a polycarbonate glass slide with dimensions of 25.0mm×75.0mm×1.2mm, immerse it in deionized water, and clean it with an ultrasonic cleaner for 5 minutes. Wipe the cleaned polycarbonate glass slide with an antistatic lint-free cloth to remove water stains. S2. Place the polycarbonate slides in a 130°C constant temperature oven and dry them for 6 hours. Remove the slides and pick out any unqualified slides with rough surfaces, cracks, damage, or air bubbles. Set aside the qualified slides for use. S3. Select a male mold with an upper base width of 120μm, a lower base width of 80μm, and a depth of 50μm. Place it on a hot press. Place a dried, flat, and transparent polycarbonate glass slide between the male mold and the upper platen of the hot press. Insert the hot press device and heating module. Set the temperature of the hot press to 150°C and maintain this temperature stably. Pressurize the hot press to a pressure of 0.5MPa and maintain the pressure at 1.5MPa for 8 minutes. After the specified time, cool the upper and lower heating blocks to near room temperature using tap water in the cooling block. Raise the hot press and demold to form a polycarbonate substrate with a microchannel structure of an average depth of 50μm. S4. Irradiate the polycarbonate substrate under X-rays with an absorbed dose of 500 Gy to complete the processing of the polycarbonate glass slide. S5. Drop water onto the surface of the treated polycarbonate substrate, observe the morphology of the water droplets, and use a water contact angle tester to test the hydrophilic properties of the polycarbonate glass slide surface to achieve performance testing and evaluation. Example 5

[0019] A method for preparing a polycarbonate plastic glass slide, comprising the following steps: S1. Select a polycarbonate glass slide with dimensions of 25.0mm×75.0mm×1.0mm, immerse it in deionized water, and clean it with an ultrasonic cleaner for 7 minutes. Wipe the cleaned polycarbonate glass slide with an antistatic lint-free cloth to remove water stains. S2. After drying the polycarbonate slides in a 130°C constant temperature oven for 10 hours, remove them and pick out any unqualified slides with rough surfaces, cracks, damage, or air bubbles. Qualified slides are ready for use. S3. Select a male mold with an upper bottom width of 110μm, a lower bottom width of 76μm, and a depth of 40μm and place it on a hot press. Place a dried, flat, and transparent polycarbonate glass slide between the male mold and the upper platen of the hot press. Put in the hot press device and heating module, set the temperature of the hot press to 150°C and maintain this temperature stably. Press the hot press to a pressure of 0.5MPa and maintain the pressure at 1.3MPa for 9 minutes. After the specified time, cool the upper and lower heating blocks to near room temperature through the tap water in the cooling block. Lift the hot press and demold to form a polycarbonate substrate with a microchannel structure with an average depth of 50μm. S4. Irradiate the polycarbonate substrate under X-rays with an absorbed dose of 800 Gy to complete the processing of the polycarbonate glass slide. S5. Drop water onto the surface of the treated polycarbonate substrate, observe the morphology of the water droplets, and use a water contact angle tester to test the hydrophilic properties of the polycarbonate glass slide surface to achieve performance testing and evaluation.

[0020] Implementation Cases Appearance after dripping water Contact angle Untreated samples Non-wetting 83.5° Example 1 infiltration 63.2° Example 2 infiltration 55.7° Example 3 infiltration 70.3° Example 4 infiltration 66.8° Example 5 infiltration 58.5° The above description is merely a preferred embodiment of the present invention and is not intended to limit the scope of the present invention. Various changes can be made to the above embodiments of the present invention. All simple and equivalent changes and modifications made in accordance with the claims and description of the present invention fall within the scope of protection of the claims of the present invention. All technical contents not described in detail in the present invention are conventional technical contents.

[0021] Among them, microfluidic technology is simple to control the surface morphology of polycarbonate, and the morphology is controllable. It can be flexibly controlled according to the needs of the application scenario. On the other hand, irradiation technology can improve the surface energy of polycarbonate under appropriate dosage conditions while ensuring the performance and strength of the glass slide itself. In the radiation treatment process, irradiation is carried out at room temperature. The method is simple and the process is controllable. The magnitude of surface hydrophilicity can be adjusted by changing the absorbed dose. The microfluidic technology combined with irradiation technology of this invention is an effective and simple method for surface modification of inorganic material interfaces and functional composite materials.

[0022] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0023] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a polycarbonate plastic glass slide, characterized in that, The preparation steps include: S1. Select polycarbonate substrates of different sizes and specifications. First, soak the polycarbonate substrates in deionized water and clean them with an ultrasonic cleaner for a certain period of time. Wipe the cleaned polycarbonate substrates with an anti-static lint-free cloth to remove water stains. S2. After drying the polycarbonate substrate in a constant temperature oven for a certain period of time, remove it and pick out the unqualified glass slides with uneven surfaces, cracks, damage, or air bubbles. Set aside the qualified polycarbonate substrate slides. S3. Select self-made male molds of different shapes and place them on the hot press. Place the dried, flat and transparent polycarbonate substrate between the male mold and the upper platen of the hot press. Put in the hot press device and heating module. Set the temperature of the hot press to the glass transition temperature. Pressurize until the pressure rises to the set value and is maintained for a certain period of time. Then, cool the upper and lower heating blocks to near room temperature by passing tap water through the cooling block. Lift the hot press and demold to obtain a polycarbonate substrate with different microchannel structures. S4. Place the polycarbonate substrate under an irradiation source to irradiate the polycarbonate substrate, and process the sample by controlling the energy density and irradiation time; S5. Drop water onto the surface of the treated polycarbonate substrate, observe the morphology of the water droplets, and use a water contact angle tester to test the hydrophilic properties of the polycarbonate glass slide surface to achieve performance testing and evaluation.

2. The method for preparing polycarbonate plastic glass slides according to claim 1, characterized in that: In step S1, the thickness of the polycarbonate slide is between 0.5-1.5 mm, and the cleaning time is 5-10 minutes.

3. The method for preparing polycarbonate plastic glass slides according to claim 1, characterized in that: In step S2, the constant temperature drying temperature is 105-130℃, and the constant temperature drying time is 4-24 hours.

4. The method for preparing polycarbonate plastic glass slides according to claim 1, characterized in that: In step S3, the set temperature of the hot press is 148-151℃, the set pressure of the hot press is 0.5-1.5MPa, and the set pressure holding time of the hot press is 2-10min.

5. The method for preparing polycarbonate plastic glass slides according to claim 1, characterized in that: In step S4, the irradiation source is plasma and X-rays. The plasma power is 100-1000W, the irradiation time is 1-10 min, the absorbed dose of X-rays is 10-1000 Gy, and the dose rate is 1-10 Gyh. −1 .

6. The method for preparing polycarbonate plastic glass slides according to claim 1, characterized in that: In step S1, the antistatic cleanroom cloth is made of double-woven polyester fiber. The wiping method is to wipe it up and down 3 times and left and right 3 times. Observe whether there are water stains on the outer surface of the polycarbonate glass slide. If water stains still exist, continue wiping.

7. The method for preparing polycarbonate plastic glass slides according to claim 1, characterized in that: In step S2, defective slides are picked out by visual inspection.

8. The method for preparing polycarbonate plastic glass slides according to claim 1, characterized in that: In step S3, the hot press is a constant temperature hot press, with 6 sets of hot press plates and a spacing of 100mm between the hot press plates.

9. The method for preparing polycarbonate plastic glass slides according to claim 1, characterized in that: The steps for using the water contact angle tester in step S5 are as follows: A1. Place a drop of the liquid to be tested onto a glass slide using an injection needle; A2. Measure hydrophilic properties with a single button press.