Rumex hanus lactobacillus fermentation equipment and method

Abstract

The invention belongs to the technical field of fermentation devices, and particularly relates to rumex hanus lactobacillus fermentation equipment. Comprising a fermentation tank; the stirring mechanism comprises a first stirring mechanism and a second stirring mechanism; the first stirring mechanism comprises a first connecting rod, a first stirring structure and a first transmission structure, the first connecting rod is vertically arranged, and the first transmission structure is arranged on the first connecting rod; the second stirring mechanism comprises a second transmission structure and a second stirring structure; the first transmission mechanism is in transmission connection with the second transmission structure; and the driving mechanism is used for driving the first connecting rod to rotate. Therefore, by arranging the first stirring structure and the second stirring structure, the fermentation liquor can be rotationally stirred, the fermentation liquor at the lower part and the fermentation liquor at the upper part can be mixed, and caking and sinking in the fermentation liquor are prevented, so that uniform mixing of the fermentation liquor is ensured.

Description

Technical Field

[0001] This invention belongs to the field of fermentation equipment technology, and in particular relates to a fermentation equipment and method for leafy grass lactic acid bacteria. Background Technology

[0002] Lactic acid bacteria (LAB) is a collective term for a group of bacteria that can ferment carbohydrates to produce large amounts of lactic acid. Lactic acid bacteria can regulate the normal flora of the gastrointestinal tract, maintain microecological balance, and improve food digestibility and biological value. They are widely used in many industries, including light industry, food, medicine, and feed. For example, they can be formulated into lactic acid bacteria products for direct consumption.

[0003] However, in the process of preparing lactic acid bacteria products, it is necessary to first obtain a fermentation broth by mixing the bacteria and the culture medium, and to take samples of the fermentation broth during the fermentation process to test its pH value, titration acidity, etc. When the fermentation broth is not uniform during the fermentation process, if the test results after sampling are qualified, it may affect the staff's misjudgment that the fermentation broth has been completed, which in turn affects the quality of the lactic acid bacteria products obtained after freeze-drying the fermentation broth. Summary of the Invention

[0004] This invention addresses the problem of uneven fermentation broth within existing fermentation equipment by proposing the following technical solution:

[0005] A fermentation device for leafy grass lactic acid bacteria includes:

[0006] A fermenter, wherein the fermenter is used to store fermentation liquid;

[0007] A stirring mechanism is located inside the fermentation tank, and the stirring mechanism includes a first stirring mechanism and a second stirring mechanism.

[0008] The first stirring mechanism includes a first connecting rod, a first stirring structure, and a first transmission structure. The first connecting rod is vertically arranged, and the first transmission mechanism is arranged on the first connecting rod.

[0009] The second stirring mechanism includes a second transmission structure and a second stirring structure. The first transmission structure is connected to the second transmission structure, and the second stirring structure is connected to the second transmission structure and moves in the vertical direction.

[0010] A driving mechanism is provided to drive the first connecting rod to rotate about its own central axis.

[0011] As a preferred embodiment of the above technical solution, the first stirring structure is provided in multiple sets, and the multiple first stirring structures are distributed on the first connecting rod along the axial direction of the first connecting rod.

[0012] As a preferred embodiment of the above technical solution, the first stirring structure includes a plurality of first rotating blades, which are circumferentially distributed around the periphery of the first connecting rod.

[0013] As a preferred embodiment of the above technical solution, the second stirring mechanism further includes a first transmission wheel, a second transmission wheel, a second connecting rod, a third connecting rod, and a belt. The third connecting rod and the second connecting rod are located on the upper and lower sides of the fermentation tank, respectively, and are rotatably connected to the fermentation tank. The axial directions of the third connecting rod and the second connecting rod are both directed towards the radial direction of the fermentation tank. The second transmission wheel is sleeved on the third connecting rod, the first transmission wheel is sleeved on the second connecting rod, and the belt is sleeved on the first transmission wheel and the second transmission wheel. The second transmission structure is connected to the first transmission wheel or the second transmission wheel, and the second stirring structure is disposed on the belt.

[0014] As a preferred embodiment of the above technical solution, the second stirring structure includes a plurality of second rotating blades, which are circumferentially distributed on the outer periphery of the belt.

[0015] As a preferred embodiment of the above technical solution, the first transmission structure is a first bevel gear, the second transmission structure is a second bevel gear, the first bevel gear is sleeved on the first connecting rod, the second bevel gear is sleeved on the second connecting rod, and the first bevel gear meshes with the second bevel gear.

[0016] As a preferred embodiment of the above technical solution, multiple second stirring mechanisms are provided, and the multiple second stirring mechanisms are arranged circumferentially around the periphery of the first connecting rod.

[0017] As a preferred embodiment of the above technical solution, the leafy grass lactic acid bacteria fermentation equipment further includes a fermentation cover, which is disposed at the upper end of the fermentation tank, and the driving mechanism is disposed inside the fermentation cover.

[0018] As a preferred embodiment of the above technical solution, the leafy grass lactic acid bacteria fermentation equipment further includes a positioning component, which is located at the center of the bottom surface of the fermentation tank. A positioning groove is provided at the center of the upper end of the positioning component, and the lower end of the first connecting rod is used to insert into the positioning groove.

[0019] A method for fermenting leafy grass with lactic acid bacteria, using the leafy grass lactic acid bacteria fermentation equipment as described in any of the above claims, comprising:

[0020] Add pure water to the fermentation tank and sterilize the pure water in the fermentation tank. The sterilization temperature is maintained between 90-95℃ and the sterilization time is 15 minutes. Add edible grass powder to the fermentation tank to obtain a culture medium. Stir the culture medium while adding it. Maintain the temperature of the culture medium between 80-85℃. After stirring evenly, cool the culture medium. The proportions of pure water and edible grass powder are 87.5% and 12.5%, respectively.

[0021] A seed culture was prepared by mixing Lactobacillus plantarum LP-C with sterile physiological saline at a ratio of 1:10. When the culture medium was cooled to 40°C, the seed culture was used to inoculate the culture medium to form a fermentation broth. The initial viable count of Lactobacillus plantarum LP-C in the culture medium was 1×10⁷ CFU / g.

[0022] Once the culture medium has cooled to 37°C, maintain the temperature of the culture medium at 37°C, start the fermenter and stir the fermentation broth at a speed of 50 rpm / min. Fermentation is carried out in cycles of 1 hour on and 3 hours off. Before each stop of stirring the fermentation broth, take a sample of the fermentation broth to measure the pH and titratable acidity and record the results. Fermentation is terminated when the pH reaches 3.7–4.0 and the titratable acidity reaches 140–160°C.

[0023] After fermentation is terminated, the fermentation liquid is placed in a freeze dryer for vacuum freeze drying. The volume of each batch of liquid is controlled at about 1L. During the freezing stage, the temperature is controlled at -35℃ and maintained for 3-4 hours. During the sublimation stage, the temperature is controlled at -15℃ and maintained for 24 hours. The final drying temperature is 30℃ and maintained for 2-3 hours. After freeze drying, the moisture content is controlled below 8% to obtain lactic acid bacteria freeze-dried powder.

[0024] The lyophilized lactic acid bacteria powder was collected, mixed evenly, and packaged in aseptic bags at a specification of 10 kg / bag. After sampling and testing, the lyophilized lactic acid bacteria powder was stored in a cold storage. The qualified test standards were as follows: the lyophilized lactic acid bacteria powder was light blue or greenish-yellow in color, had a uniform powder texture without lumps, and had a unique odor. At the same time, it was also required to test for coliform bacteria according to GB 4789.3 standard, i.e., coliform bacteria ≤3 MPN / g, and for mold and yeast according to GB4789.15 standard, i.e., mold and yeast ≤50 CFU / g.

[0025] The beneficial effects of this invention are as follows:

[0026] (1) By setting a first connecting rod, a first stirring structure, and a driving mechanism inside the fermentation tank, the driving mechanism drives the first connecting rod to rotate, thereby driving the first stirring structure to rotate inside the fermentation tank, which can mix the fermentation liquid at the same height in the fermentation tank evenly. At the same time, the second stirring structure is connected to the first connecting rod through a second transmission structure and a first transmission structure. When the first connecting rod rotates, it can drive the second stirring structure to move in the vertical direction, which can mix the fermentation liquid at the top and bottom of the fermentation tank evenly. Thus, by setting the first stirring structure and the second stirring structure, the fermentation liquid can be rotated and stirred, and the fermentation liquid at the bottom and top can be mixed, preventing lumps from settling in the fermentation liquid, thereby ensuring that the fermentation liquid is mixed evenly.

[0027] (2) At the same time, the second stirring structure and the first stirring structure are connected by the first transmission structure and the second transmission structure, which means that only one driving mechanism can be set to drive the first stirring structure and the second stirring structure at the same time, which is beneficial to reduce the structure of the leafy grass lactic acid bacteria fermentation equipment.

[0028] (3) Since the first transmission wheel or the second transmission wheel is connected to the first connecting rod through the second transmission structure and the first transmission structure, the first connecting rod drives the first transmission structure, the second transmission structure, the first transmission wheel and the second transmission wheel in sequence during the rotation of the first connecting rod. The first transmission wheel and the second transmission wheel are respectively sleeved on the second connecting rod and the third connecting rod, and belts are sleeved on the first transmission wheel and the second transmission wheel, which can drive the belt to rotate. The second stirring structure is set on the belt. During the rotation of the belt, the second stirring structure can be driven to move back and forth in the vertical direction, thereby realizing the stirring and mixing of the upper fermentation liquid and the lower fermentation liquid, thus ensuring the function of the second stirring mechanism.

[0029] (4) The first transmission structure is configured as a first bevel gear, which is sleeved on the first connecting rod. Rotation of the first connecting rod drives the first bevel gear to rotate. The second transmission structure is configured as a second bevel gear, with the first and second bevel gear meshing. Rotation of the first bevel gear drives the second bevel gear to rotate, thereby driving the first transmission wheel, the second transmission wheel, and the belt to rotate. This allows multiple second rotating blades on the belt to act simultaneously on the fermentation liquid, ensuring uniform mixing. This guarantees the effectiveness of both the first and second transmission structures. Attached Figure Description

[0030] Figure 1 The diagram shown is a structural schematic of the leafy green lactic acid bacteria device in the embodiment;

[0031] Figure 2 The diagram shown is a schematic representation of the internal structure of the leafy green lactic acid bacteria device in the embodiment.

[0032] In the diagram: 1. Fermentation tank; 21. First connecting rod; 22. First stirring structure; 23. First transmission structure; 31. Second transmission structure; 32. Second stirring structure; 331. First transmission wheel; 332. Second transmission wheel; 333. Second connecting rod; 334. Third connecting rod; 335. Belt; 4. Drive mechanism; 5. Fermentation cover; 6. Positioning component. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments.

[0034] Based on existing technologies, fermentation equipment that only rotates and mixes the fermentation broth without mixing the fermentation broth vertically within the fermenter 1, such as... Figure 1 and Figure 2 As shown, an embodiment of the present invention provides a fermentation device for leafy green lactic acid bacteria, comprising:

[0035] Fermentation tank 1 is used to store fermentation liquid;

[0036] A stirring mechanism is located inside the fermentation tank 1, and the stirring mechanism includes a first stirring mechanism and a second stirring mechanism.

[0037] The first stirring mechanism includes a first connecting rod 21, a first stirring structure 22, and a first transmission structure 23. The first connecting rod 21 is vertically arranged, and the first transmission mechanism is arranged on the first connecting rod 21.

[0038] The second stirring mechanism includes a second transmission structure 31 and a second stirring structure 32. The first transmission mechanism is connected to the second transmission structure 31, and the second stirring structure 32 is connected to the second transmission structure 31 and moves in the vertical direction.

[0039] Drive mechanism 4 is used to drive the first connecting rod 21 to rotate around its own central axis.

[0040] In this embodiment, the fermentation broth formed from leafy greens and lactic acid bacteria is placed inside the fermentation tank 1. By activating the drive mechanism 4, the first connecting rod 21 can be rotated. Since the first stirring structure 22 is mounted on the first connecting rod 21, the rotation of the first connecting rod 21 simultaneously drives the first stirring structure 22 to rotate within the fermentation tank 1, thereby stirring the fermentation broth using the first stirring structure 22. Simultaneously, the first transmission mechanism is mounted on the first connecting rod 21, and the second stirring structure 32 is hinged to the first transmission mechanism via the second transmission mechanism. This allows the second stirring structure 32 to rotate synchronously along the vertical direction of the fermentation tank 1, thereby stirring the fermentation broth using the second stirring structure 32.

[0041] In summary, by installing a first connecting rod 21, a first stirring structure 22, and a driving mechanism 4 inside the fermentation tank 1, the driving mechanism 4 drives the first connecting rod 21 to rotate, thereby causing the first stirring structure 22 to rotate within the fermentation tank 1. This allows for uniform mixing of the fermentation liquid at the same height within the fermentation tank 1. Simultaneously, the second stirring structure 32 is connected to the first connecting rod 21 via a second transmission structure 31 and a first transmission structure 23. When the first connecting rod 21 rotates, it drives the second stirring structure 32 to move vertically, thus uniformly mixing the fermentation liquid at the top and bottom of the fermentation tank 1. Therefore, by installing the first stirring structure 22 and the second stirring structure 32, both the fermentation liquid can be rotated and stirred, and the fermentation liquid at the bottom and top can be mixed, preventing clumps from settling and ensuring uniform mixing of the fermentation liquid. Meanwhile, the second stirring structure 32 and the first stirring structure 22 are connected by the first transmission structure 23 and the second transmission structure 31, which means that only one drive mechanism 4 is needed to drive the first stirring structure 22 and the second stirring structure 32 at the same time, which helps to reduce the structure of the leafy grass lactic acid bacteria fermentation equipment.

[0042] Optionally, such as Figure 1 and Figure 2 As shown, multiple sets of the first stirring structure 22 are provided, and the multiple first stirring structures 22 are distributed on the first connecting rod 21 along the axial direction of the first connecting rod 21.

[0043] In this embodiment, multiple sets of first stirring structures 22 are provided, and the multiple first stirring structures 22 are distributed along the axial direction of the first connecting rod 21. The multiple first stirring structures 22 can thoroughly stir the fermentation liquid in the fermentation tank 1, which is beneficial to further improve the uniformity of stirring the fermentation liquid.

[0044] Optionally, such as Figure 1 and Figure 2 As shown, the first stirring structure 22 includes a plurality of first rotating blades, which are distributed circumferentially around the first connecting rod 21.

[0045] Specifically, multiple first rotating blades are evenly distributed around the periphery of the first connecting rod 21.

[0046] In this embodiment, the first stirring structure 22 is configured as a plurality of first rotating blades, and the plurality of first rotating blades are distributed circumferentially around the first connecting rod 21. When the first connecting rod 21 rotates, the plurality of first rotating blades can act on the fermentation liquid simultaneously, so that the fermentation liquid is mixed evenly.

[0047] Optionally, such as Figure 1 and Figure 2As shown, the second stirring mechanism further includes a first transmission wheel 331, a second transmission wheel 332, a second connecting rod 333, a third connecting rod 334, and a belt 335. The third connecting rod 334 and the second connecting rod 333 are respectively located on the upper and lower sides of the fermentation tank 1, and are both rotatably connected to the fermentation tank 1. The axial directions of the third connecting rod 334 and the second connecting rod 333 both face the radial direction of the fermentation tank 1. The second transmission wheel 332 is sleeved on the third connecting rod 334, the first transmission wheel 331 is sleeved on the second connecting rod 333, the belt 335 is sleeved on the first transmission wheel 331 and the second transmission wheel 332, the second transmission structure 31 is in transmission connection with the first transmission wheel 331 or the second transmission wheel 332, and the second stirring structure 32 is arranged on the belt 335.

[0048] In this embodiment, since the first transmission wheel 331 or the second transmission wheel 332 is in transmission connection with the first connecting rod 21 through the second transmission structure 31 and the first transmission structure 23, when the first connecting rod 21 rotates, it sequentially drives the first transmission structure 23, the second transmission structure 31, and the first transmission wheel 331 and the second transmission wheel 332. The first transmission wheel 331 and the second transmission wheel 332 are respectively sleeved on the second connecting rod 333 and the third connecting rod 334, and the belt 335 is sleeved on the first transmission wheel 331 and the second transmission wheel 332, so that the belt 335 can be driven to rotate. And since the second stirring structure 32 is arranged on the belt 335, the belt 335 can drive the second stirring structure 32 to reciprocate vertically during rotation, so as to realize the stirring and mixing of the upper fermentation liquid and the lower fermentation liquid, thus ensuring the function of the second stirring mechanism.

[0049] At the same time, the first connecting rod 21 can be set as a horizontally placed "convex" - shaped structure. Among them, the first connecting rod 21 includes a first vertical rod, a first horizontal rod, and a second vertical rod. The first vertical rod, the first horizontal rod, and the second vertical rod are connected end to end in sequence. The first transmission structure 23 can be set as a sleeve coaxially arranged with the second vertical rod, the second transmission structure 31 can be set as a connecting rod, and the second stirring mechanism is slidably connected to the fermentation tank 1 in the vertical direction. One end of the connecting rod is hinged to the sleeve, and the other end is hinged to the second stirring mechanism.

[0050] In this embodiment, when the driving mechanism 4 drives the first connecting rod 21 to rotate, when the sleeve is driven away from the second stirring mechanism, the second stirring mechanism moves downward. Similarly, when the sleeve is driven close to the second stirring mechanism, the second stirring mechanism moves upward. Using the second stirring mechanism to mix the fermentation liquid in the up - down direction can also ensure the effect of the second stirring mechanism.

[0051] Optionally, as Figure 1 and Figure 2As shown, the second stirring structure 32 includes a plurality of second rotating blades, which are distributed circumferentially on the outer periphery of the belt 335.

[0052] Specifically, multiple second rotating blades are evenly distributed around the outer periphery of the belt 335.

[0053] In this embodiment, the second stirring structure 32 is configured as multiple second rotating blades, and the multiple second rotating blades are distributed circumferentially on the outer periphery of the belt 335. When the belt 335 rotates, the multiple second rotating blades can act on the fermentation liquid simultaneously, so that the fermentation liquid is mixed evenly.

[0054] Optionally, such as Figure 1 and Figure 2 As shown, the first transmission structure 23 is a first bevel gear, and the second transmission structure 31 is a second bevel gear. The first bevel gear is sleeved on the first connecting rod 21, and the second bevel gear is sleeved on the second connecting rod 333. The first bevel gear and the second bevel gear mesh.

[0055] In this embodiment, the first transmission structure 23 is configured as a first bevel gear, which is sleeved on the first connecting rod 21. Rotation of the first connecting rod 21 drives the first bevel gear to rotate. The second transmission structure 31 is configured as a second bevel gear, and the first and second bevel gears mesh. Rotation of the first bevel gear drives the second bevel gear to rotate, thereby driving the first transmission wheel 331, the second transmission wheel 332, and the belt 335 to rotate. This allows multiple second rotating blades on the belt 335 to simultaneously act on the fermentation liquid, ensuring uniform mixing. This guarantees the effective operation of the first transmission structure 23 and the second transmission structure 31.

[0056] Optionally, such as Figure 1 and Figure 2 As shown, multiple second stirring mechanisms are provided, and the multiple second stirring mechanisms are arranged circumferentially around the first connecting rod 21.

[0057] In this embodiment, multiple second stirring mechanisms can be provided, which can facilitate the uniform stirring of the fermentation liquid in the fermentation tank 1 in the vertical direction.

[0058] Optionally, such as Figure 1 and Figure 2 As shown, the leafy grass lactic acid bacteria fermentation equipment also includes a fermentation cover 5, which is set at the upper end of the fermentation tank 1, and a drive mechanism 4 is set inside the fermentation cover 5.

[0059] Specifically, the drive mechanism 4 is a motor, wherein the output shaft of the motor faces downward and is connected to the first connecting rod 21.

[0060] In this embodiment, by setting a fermentation cover 5 and placing the drive mechanism 4 inside the fermentation cover 5, it is possible to prevent the drive mechanism 4 from malfunctioning when exposed to water, reduce unexpected situations during the fermentation process, and ensure the smooth progress of the fermentation process.

[0061] Optionally, such as Figure 1 As shown, the leafy grass lactic acid bacteria fermentation equipment also includes a positioning component 6, which is set at the center of the bottom surface of the fermentation tank 1. A positioning groove is opened at the center of the upper end of the positioning component 6, and the lower end of the first connecting rod 21 is used to insert into the positioning groove.

[0062] In this embodiment, by setting a positioning member 6 at the center of the fermentation tank 1 and opening a positioning groove on the positioning member 6, the positioning groove is located at the center of the positioning member 6. When the lower end of the first connecting rod 21 is inserted into the positioning groove, it can be ensured that the central axis of the first connecting rod 21 coincides with the central axis of the fermentation tank 1.

[0063] Another embodiment of the present invention provides a method for fermenting leafy grass lactic acid bacteria, characterized in that it uses the above-described leafy grass lactic acid bacteria fermentation equipment, including:

[0064] Add pure water to fermenter 1 and sterilize the pure water in fermenter 1. The sterilization temperature is maintained between 90-95℃ and the sterilization time is 15 minutes. Add edible grass powder to fermenter 1 to obtain culture medium. Stir the culture medium while adding it and maintain the temperature of the culture medium between 80-85℃. After stirring evenly, cool the culture medium. The proportions of pure water and edible grass powder are 87.5% and 12.5%, respectively.

[0065] A seed culture was prepared by mixing Lactobacillus plantarum LP-C with sterile physiological saline at a ratio of 1:10. When the culture medium was cooled to 40°C, the seed culture was used to inoculate the culture medium to form a fermentation broth. The initial viable count of Lactobacillus plantarum LP-C in the culture medium was 1×10⁷ CFU / g.

[0066] Once the culture medium has cooled to 37°C, maintain the temperature of the culture medium at 37°C, start fermenter 1 and stir the fermentation broth at a speed of 50 rpm / min. Fermentation is carried out in cycles of 1 hour on and 3 hours off. Before stopping the stirring of the fermentation broth each time, take a sample of the fermentation broth to measure the pH and titratable acidity and record the results. Terminate fermentation when the pH reaches 3.7-4.0 and the titratable acidity reaches 140-160°C.

[0067] After fermentation is terminated, the fermentation liquid is placed in a freeze dryer for vacuum freeze drying. The volume of each tray of liquid is controlled at about 1L. During the freezing stage, the temperature is controlled at -35℃ and maintained for 3-4 hours. During the sublimation stage, the temperature is controlled at -15℃ and maintained for 24 hours. The final drying temperature is 30℃ and maintained for 2-3 hours. After freeze drying, the moisture content is controlled below 8% to obtain lactic acid bacteria freeze-dried powder.

[0068] The lyophilized lactic acid bacteria powder was collected, mixed evenly, and packaged in aseptic bags at a specification of 10kg / bag. After sampling and testing, the lyophilized lactic acid bacteria powder was stored in a cold storage. The qualified standards for the lyophilized lactic acid bacteria powder are as follows: the color of the powder is light blue or greenish-yellow, the texture is uniform powder without lumps, and it has a unique odor. At the same time, the coliform bacteria were tested according to GB 4789.3 standard, i.e., coliform bacteria ≤3MPN / g, and the molds and yeasts were tested according to GB 4789.15 standard, i.e., molds and yeasts ≤50CFU / g.

[0069] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it.

Claims

1. A fermentation device for leafy green lactic acid bacteria, characterized in that, include: Fermentation tank (1), wherein the fermentation tank (1) is used to store fermentation liquid; A stirring mechanism is located inside the fermentation tank (1), and the stirring mechanism includes a first stirring mechanism and a second stirring mechanism; The first stirring mechanism includes a first connecting rod (21), a first stirring structure (22), and a first transmission structure (23). The first connecting rod (21) is vertically arranged, and the first transmission mechanism is arranged on the first connecting rod (21). The second stirring mechanism includes a second transmission structure (31) and a second stirring structure (32). The first transmission structure is connected to the second transmission structure (31), and the second stirring structure (32) is connected to the second transmission structure (31) and moves in the vertical direction. The driving mechanism (4) is used to drive the first connecting rod (21) to rotate around its own central axis.

2. The leafy grass lactic acid bacteria fermentation equipment according to claim 1, characterized in that, The first stirring structure (22) is provided in multiple sets, and multiple first stirring structures (22) are distributed on the first connecting rod (21) along the axial direction of the first connecting rod (21).

3. The leafy grass lactic acid bacteria fermentation equipment according to claim 2, characterized in that, The first stirring structure (22) includes a plurality of first rotating blades, which are distributed circumferentially around the first connecting rod (21).

4. The leafy grass lactic acid bacteria fermentation equipment according to claim 1, characterized in that, The second stirring mechanism further includes a first transmission wheel (331), a second transmission wheel (332), a second connecting rod (333), a third connecting rod (334), and a belt (335). The third connecting rod (334) and the second connecting rod (333) are located on the upper and lower sides of the fermentation tank (1) respectively, and are rotatably connected to the fermentation tank (1). The axial directions of the third connecting rod (334) and the second connecting rod (333) are both directed toward the radial direction of the fermentation tank (1). The second transmission wheel (332) is sleeved on the third connecting rod (334), the first transmission wheel (331) is sleeved on the second connecting rod (333), and the belt (335) is sleeved on the first transmission wheel (331) and the second transmission wheel (332). The second transmission structure (31) is connected to the first transmission wheel (331) or the second transmission wheel (332) for transmission. The second stirring structure (32) is disposed on the belt (335).

5. The leafy grass lactic acid bacteria fermentation equipment according to claim 4, characterized in that, The second stirring structure (32) includes a plurality of second rotating blades, which are circumferentially distributed on the outer periphery of the belt (335).

6. The leafy grass lactic acid bacteria fermentation equipment according to claim 5, characterized in that, The first transmission structure (23) is a first bevel gear, and the second transmission structure (31) is a second bevel gear. The first bevel gear is sleeved on the first connecting rod (21), and the second bevel gear is sleeved on the second connecting rod (333). The first bevel gear meshes with the second bevel gear.

7. The leafy grass lactic acid bacteria fermentation equipment according to claim 1, characterized in that, Multiple second stirring mechanisms are provided, and the multiple second stirring mechanisms are arranged circumferentially around the first connecting rod (21).

8. The leafy grass lactic acid bacteria fermentation equipment according to claim 1, characterized in that, It also includes a fermentation cover (5), which is disposed at the upper end of the fermentation tank (1), and the driving mechanism (4) is disposed inside the fermentation cover (5).

9. The leafy grass lactic acid bacteria fermentation equipment according to claim 1, characterized in that, It also includes a positioning component (6), which is located at the center of the bottom surface of the fermenter (1). A positioning groove is provided at the center of the upper end of the positioning component (6), and the lower end of the first connecting rod (21) is used to insert into the positioning groove.

10. A method for fermenting leafy green lactic acid bacteria, characterized in that, The leafy grass lactic acid bacteria fermentation equipment as described in any one of claims 1-9 includes: Add pure water to the fermentation tank (1) and sterilize the pure water in the fermentation tank (1). The sterilization temperature is maintained between 90-95℃ and the sterilization time is 15 minutes. Add leafy grass powder to the fermentation tank (1) to obtain a culture medium. Stir the culture medium while adding it. Maintain the temperature of the culture medium between 80-85℃. After stirring evenly, cool the culture medium. The proportions of pure water and leafy grass powder are 87.5% and 12.5%, respectively. A seed culture was prepared by mixing Lactobacillus plantarum LP-C with sterile physiological saline at a ratio of 1:

10. When the culture medium was cooled to 40°C, the seed culture was used to inoculate the culture medium to form a fermentation broth. The initial viable count of Lactobacillus plantarum LP-C in the culture medium was 1×10⁷ CFU / g. When the culture medium is cooled to 37°C, the temperature of the culture medium is maintained at 37°C. The fermentation tank (1) is started and the fermentation liquid is stirred at a speed of 50 rpm / min. The fermentation is carried out in a cycle of 1 hour and 3 hours. Before each stop of stirring the fermentation liquid, the pH and titration acidity of the fermentation liquid are measured and recorded. Fermentation is terminated when the pH reaches 3.7 to 4.0 and the titration acidity reaches 140 to 160°. After fermentation is terminated, the fermentation liquid is placed in a freeze dryer for vacuum freeze drying. The volume of each batch of liquid is controlled at about 1L. During the freezing stage, the temperature is controlled at -35℃ and maintained for 3-4 hours. During the sublimation stage, the temperature is controlled at -15℃ and maintained for 24 hours. The final drying temperature is 30℃ and maintained for 2-3 hours. After freeze drying, the moisture content is controlled below 8% to obtain lactic acid bacteria freeze-dried powder. The lyophilized lactic acid bacteria powder was collected, mixed evenly, and packaged in aseptic bags at a specification of 10 kg / bag. After sampling and testing, the lyophilized lactic acid bacteria powder was stored in a cold storage. The qualified test standards were as follows: the lyophilized lactic acid bacteria powder was light blue or greenish-yellow in color, had a uniform powder texture without lumps, and had a unique odor. At the same time, it was also required to test for coliform bacteria according to GB 4789.3 standard, i.e., coliform bacteria ≤3 MPN / g, and for mold and yeast according to GB4789.15 standard, i.e., mold and yeast ≤50 CFU / g.

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