Transfer device based on strain preservation

Abstract

The transfer device comprises a test tube and a cover body, the cover body can cover the open end of the test tube, the lower end of the cover body is provided with a supporting rod, the lower end of the supporting rod is connected with a placing shell, the placing shell is in a state that the front end is open, and the bottom in the placing shell is in a horizontal state; a fixing assembly used for fixing the fungus blocks is arranged in the containing shell. When the device is used, a fungus block is placed in the placing shell, the fungus block is fixed by using the fixing assembly, then the fungus block is placed in a test tube to be stored, the placing shell is directly taken out when the fungus block is taken out, placing and taking are very convenient, contact with the wall of the test tube can be avoided, and the possibility of pollution is reduced.

Description

Technical Field

[0001] This invention relates to the field of microbial preservation technology, and in particular to a transfer device based on strain preservation. Background Technology

[0002] In microbiological research, industrial fermentation, and clinical testing, the long-term stable preservation of microbial strains is crucial for ensuring experimental reproducibility, production continuity, and the safety of biological resources. Currently, commonly used methods for strain preservation include slant culture cryopreservation, glycerol cryopreservation, freeze-drying, and liquid nitrogen cryopreservation. Among these, removing the bacterial blocks from solid culture media and transferring them to cryovials for preservation at -80°C or in liquid nitrogen is a simple and widely applicable method.

[0003] In this process, the mycelium block is typically transferred to the cryovial using an inoculation spatula (or inoculation loop, inoculation needle). However, in practice, this transfer step has significant technical drawbacks: due to the adhesiveness and moisture content of the mycelium block, it easily adheres to the surface of the metal or plastic inoculation spatula, making it difficult for it to slide naturally to the bottom of the cryovial under gravity. Operators often need to press the inoculation spatula firmly against the inner wall of the cryovial and repeatedly scrape or shake it to dislodge the mycelium block, making insertion and removal very troublesome and affecting work efficiency. Summary of the Invention

[0004] The purpose of this invention is to provide a transfer device based on bacterial culture preservation, which involves placing bacterial blocks inside a placement shell, fixing them with a fixing component, and then placing them in a test tube for preservation.

[0005] The above-mentioned technical objective of the present invention is achieved through the following technical solution: A transfer device based on microbial culture preservation includes a test tube and a cap. The cap can be placed on the open end of the test tube. The lower end of the cap is provided with a support rod. The lower end of the support rod is connected to a placement shell. The placement shell is open at the front end. The bottom of the placement shell is horizontal. The placement shell is provided with a fixing component for fixing the microbial block.

[0006] By adopting the above technical solution, the bacterial block is placed in the placement shell, fixed with the fixing component, and then placed in the test tube for storage. When taking it out, the placement shell can be taken out directly. The placement and use are very convenient, and it can also avoid contact with the test tube wall, reducing the possibility of contamination.

[0007] A further configuration of the present invention is as follows: the upper end of the support rod is rotatably connected to the middle part of the cover, the support rod can rotate along its own axis, the upper half of the support rod has a fixing hole, the lower half of the support rod has a sliding hole, the lower end of the fixing hole is connected to the upper end of the sliding hole, the fixing assembly includes a pressing plate, a sliding rod and a screw, the screw is inserted into the fixing hole, the screw and the fixing hole form a rotational fit, the upper end of the screw is connected to the cover, the sliding rod is inserted into the sliding hole, the sliding rod and the sliding hole form a sliding fit, the lower end of the screw is inserted into the upper end of the sliding rod, the screw is threadedly connected to the cover, the pressing plate is disposed in the placement shell, the pressing plate is slidably connected to the placement shell, and the lower end of the sliding rod is rotatably connected to the upper end of the pressing plate.

[0008] By adopting the above technical solution, the shell is fixed in place during use. Rotating the shell can drive the screw to rotate, which in turn drives the slide bar to move downward, thereby pushing the pressing plate to press the bacterial block downward.

[0009] A further feature of the present invention is that a first flexible pad is provided at the bottom of the placement shell, and the upper end of the first flexible pad is in a continuous wavy shape.

[0010] By adopting the above technical solution, the protection of the bacterial block is guaranteed when pressing.

[0011] A further feature of the present invention is that the lower end of the pressing plate is provided with a second flexible pad, and the lower end of the second flexible pad is in a continuous wavy shape.

[0012] By adopting the above technical solution, the protection of the bacterial block is guaranteed when pressing.

[0013] A further feature of the present invention is that a baffle is provided at the front end of the pressing plate.

[0014] By adopting the above technical solution, the movement of the mycelium blocks is restricted, preventing them from falling out of the container.

[0015] A further feature of the present invention is that two symmetrical limiting plates are provided inside the test tube near the opening end, which can restrict the rotation of the placement shell when the placement shell is inserted between the two limiting plates.

[0016] By adopting the above technical solution, after the mycelium block is placed in the placement shell, the placement shell is placed between two limiting plates. By rotating the shell, the pressing plate presses the mycelium block. The same operation can be used to remove it, and the pressing plate presses the mycelium block.

[0017] In summary, the present invention has the following beneficial effects: Firstly, when using this invention, the bacterial block is placed inside the placement shell, fixed with a fixing component, and then placed into a test tube for storage. When taking it out, the placement shell can be removed directly. Placement and retrieval are very convenient, and it can also avoid contact with the test tube wall, reducing the possibility of contamination.

[0018] Secondly, the fixing component in this invention can fix the placement shell and rotate the cover to move the pressing plate downward, thereby clamping and fixing the bacterial block. The method of fixing the placement shell can be carried out with the help of the limiting plate inside the test tube. After the placement shell is placed in the two limiting plates, the rotation of the placement shell can be restricted, thus completing the fixing and loosening of the bacterial block and avoiding contamination caused by the use of other tools. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a cross-sectional view of the present invention; Figure 3 This is the present invention. Figure 2 Enlarged view of point A in the middle; Figure 4 This is a cross-sectional view of the fixing component in this invention.

[0020] In the diagram: 1. Test tube; 2. Cover; 3. Support rod; 31. Fixing hole; 32. Sliding hole; 4. Placement shell; 5. Fixing component; 51. Pressing plate; 52. Sliding rod; 53. Screw; 54. Baffle; 61. First flexible pad; 62. Second flexible pad; 7. Limiting plate. Detailed Implementation

[0021] The present invention will be further described in detail below with reference to the accompanying drawings.

[0022] In the description of this invention, it should be understood that the terms "upper", "lower", "left", "right", "front", "rear", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0023] Furthermore, terms such as "horizontal" and "vertical" do not imply that components must be absolutely horizontal or suspended, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," not that the structure must be completely horizontal, but can be slightly tilted.

[0024] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, a direct connection, or an indirect connection through an intermediate medium; or they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0025] Example: A transfer device based on strain preservation, such as... Figure 1 As shown, it includes a test tube 1 and a lid 2. The lid 2 can be placed on the open end of the test tube 1. The test tube 1 is made of a transparent material to facilitate observation of its internal contents. Figure 2 and Figure 3 As shown, a support rod 3 is provided at the lower end of the cover 2, and a placement shell 4 is connected to the lower end of the support rod 3. The placement shell 4 is open at the front end, and the bottom of the placement shell 4 is horizontal. A fixing component 5 for fixing the mycelium block is provided inside the placement shell 4. In use, the mycelium block can be placed into the placement shell 4, then fixed with the fixing component 5, and finally placed into the test tube 1. The cover 2 is then used to seal the test tube 1, and the test tube is placed in an incubator for storage.

[0026] The upper end of the support rod 3 is rotatably connected to the middle of the cover 2. The support rod 3 can rotate along its own axis. The upper half of the support rod 3 has a fixing hole 31, and the lower half of the support rod 3 has a sliding hole 32. The lower end of the fixing hole 31 is connected to the upper end of the sliding hole 32. The fixing component 5 includes a pressing plate 51, a sliding rod 52, and a screw 53. The screw 53 is inserted into the fixing hole 31, and the screw 53 and the fixing hole 31 form a rotational fit. The upper end of the screw 53 is connected to the cover 2. The sliding rod 52 is inserted into the sliding hole 32, and the sliding rod 52 and the sliding hole 32 form a sliding fit. The lower end of the screw 53 is inserted into the upper end of the sliding rod 52. The screw 53 is threadedly connected to the upper end of the pressing plate 51, which is located inside the placement shell 4. The pressing plate 51 is slidably connected to the placement shell 4, and the lower end of the sliding rod 52 is rotatably connected to the upper end of the pressing plate 51. When in use, first fix the placement shell 4 to prevent it from rotating, then rotate the cover 2 to make the screw 53 rotate, thereby driving the slide bar 52 to slide up and down, so that the pressing plate 51 presses and fixes the bacterial block inside the placement shell 4.

[0027] like Figure 4 As shown, a first flexible pad 61 is provided at the bottom inside the housing 4, and the upper end of the first flexible pad 61 is continuously wavy. A second flexible pad 62 is provided at the lower end of the pressing plate 51, and the lower end of the second flexible pad 62 is continuously wavy. The first flexible pad 61 and the second flexible pad 62 can protect the clamped mycelium block. The wavy shape of the first flexible pad 61 and the second flexible pad 62 can reduce contact with the mycelium block.

[0028] To prevent the bacterial block from falling off due to insufficient pressing pressure and tilting of the test tube 1, a baffle 54 is provided at the front end of the pressing plate 51 to improve the stability of the fixation.

[0029] Two symmetrical limiting plates 7 are provided inside the test tube 1 near the opening. When the placement shell 4 is inserted between the two limiting plates 7, it can restrict the rotation of the placement shell 4. The setting of the limiting plates 7 can avoid the use of other tools, thereby reducing the contamination of the bacterial block.

[0030] Instructions for use: After cutting the mycelium block using a cutting tool, place the mycelium block into the placement shell 4 using the cutting tool. Then, insert the placement shell 4 into the test tube 1, tilting the test tube 1 so that the open end of the placement shell 4 faces upwards. Secure the placement shell 4 between the two limiting plates 7. Rotate the cap 2 to allow the pressing plate 51 to gently press and fix the mycelium block. Then, continue inserting the placement shell 4 into the bottom of the test tube 1, making the test tube 1 vertical, and then rotate the cap 2 again to seal the test tube 1. To remove the mycelium block, rotate the cap 2 between the two limiting plates 7 to release the pressure of the fixing component 5 on the mycelium block, then remove the placement shell 4. Use a sterilized tool to remove the mycelium block from the placement shell 4.

[0031] This specific embodiment is merely an explanation of the present invention and is not intended to limit the invention. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A transfer device based on microbial strain preservation, comprising a test tube (1) and a cap (2), wherein the cap (2) can be placed on the open end of the test tube (1), characterized in that: The lower end of the cover (2) is provided with a support rod (3), and the lower end of the support rod (3) is connected to a placement shell (4). The placement shell (4) is open at the front end, and the bottom of the placement shell (4) is horizontal. The placement shell (4) is provided with a fixing component (5) for fixing the fungal block.

2. The transfer device based on strain preservation according to claim 1, characterized in that: The upper end of the support rod (3) is rotatably connected to the middle part of the cover (2). The support rod (3) can rotate along its own axis. The upper half of the support rod (3) is provided with a fixing hole (31), and the lower half of the support rod (3) is provided with a sliding hole (32). The lower end of the fixing hole (31) is connected to the upper end of the sliding hole (32). The fixing component (5) includes a pressing plate (51), a sliding rod (52), and a screw (53). The screw (53) is inserted into the fixing hole (31), and the screw (53) is connected to the fixing hole (2). 31) A rotating fit is formed, the upper end of the screw (53) is connected to the cover (2), the slide rod (52) is inserted into the slide hole (32), the slide rod (52) and the slide hole (32) form a sliding fit, the lower end of the screw (53) is inserted into the upper end of the slide rod (52), the screw (53) is threaded to the cover, the pressing plate (51) is provided in the placement shell (4), the pressing plate (51) is slidably connected to the placement shell (4), and the lower end of the slide rod (52) is rotatably connected to the upper end of the pressing plate (51).

3. The transfer device based on strain preservation according to claim 2, characterized in that: The bottom of the placement shell (4) is provided with a first flexible pad (61), and the upper end of the first flexible pad (61) is in a continuous wave shape.

4. The transfer device based on strain preservation according to claim 3, characterized in that: The lower end of the pressing plate (51) is provided with a second flexible pad (62), and the lower end of the second flexible pad (62) is in a continuous wave shape.

5. The transfer device based on strain preservation according to claim 4, characterized in that: The front end of the pressing plate (51) is provided with a baffle (54).

6. The transfer device based on strain preservation according to claim 5, characterized in that: The test tube (1) is provided with two symmetrical limiting plates (7) near the opening end. When the placement shell (4) is inserted between the two limiting plates (7), the rotation of the placement shell (4) can be restricted.

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