Flow cytometer convenient for fluid infusion

Abstract

The invention relates to the technical field of flow cytometers, and discloses a flow cytometer convenient for liquid supplementation, which comprises a body, the side surface of the body is fixedly connected with a liquid adding box body, the right side of the liquid adding box body is communicated with a liquid inlet pipe, the back of the liquid adding box body is provided with a first conveying pipe, the body is internally provided with a shaking mechanism, and the shaking mechanism is internally provided with a second conveying pipe. The uniform shaking mechanism comprises a motor, the motor is fixedly connected to the bottom of the body, and intermittent rotation of a placing disc can be realized through transmission of a notch disc, a fixed rod and a rectangular disc, so that starting of next-time uniform mixing is realized, and a shearing force is suddenly applied from a static state. The instant impact from static to dynamic is more effective than continuous mild vortexes for cell agglomerates which are scattered at the bottom of the tube and just start to gather or adhere to the wall. The settling process is equivalent to resetting the settling process again and again, and the settling process is collapsed with the maximum force during resetting each time.

Description

Technical Field

[0001] This invention relates to the field of flow cytometry technology, specifically to a flow cytometer that facilitates fluid resuscitation. Background Technology

[0002] A flow cytometer is a device for automated analysis and sorting of cells. It can rapidly measure, store, and display a range of important biophysical and chemophysical parameters of dispersed cells suspended in a liquid, and can sort specific cell subpopulations from the liquid based on a pre-selected range of parameters.

[0003] For example, CN219455874U discloses a flow cytometer that facilitates fluid replenishment, relating to the field of flow cytometry technology. It includes a flow cytometer body, with a sample feeder and a sheath fluid feeder on the side of the body. An inlet tube is located on the top of the inner wall of the body. An adjustment device is installed inside the flow cytometer body, including a motor. A connecting column is located on the top of the motor, a main gear is located in the middle of the connecting column, and multiple secondary gears are located on the side of the main gear. An outer ring is located outside the secondary gears. When the motor is turned on, the motor shaft drives a drive rod to rotate via the connecting column. The drive rod drives the secondary gears to rotate around the outer surface of the main gear via a short column. The secondary gears drive a tray to rotate around the main gear via the short column. This design allows operators to quickly remove samples from the flow cytometer body, reducing the degree of sample contamination by airborne bacteria and oxidation by oxygen, thus improving sample detection accuracy.

[0004] When using a container to hold a large number of samples, cells will settle during the settling process, resulting in inconsistent cell concentrations in the early and later stages of sample loading, which in turn affects the detection results. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a flow cytometer that facilitates fluid resuscitation, addressing the shortcomings of the prior art.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is: a flow cytometer for easy fluid replenishment, comprising a main body, a liquid addition tank fixedly connected to the side of the main body, an inlet pipe connected to the right side of the liquid addition tank, a first delivery pipe provided at the back of the liquid addition tank, a shaking mechanism provided inside the main body, the shaking mechanism including a motor, the motor fixedly connected to the bottom of the main body, the output end of the motor fixedly connected to a first rotating rod via a coupling, the first rotating rod movably penetrating the main body and extending into it, and a notched disc fixedly connected to the top of the first rotating rod. A crossbar is fixedly connected to the outer wall of the notched disc, and a first fixed rod is fixedly connected to the top of the crossbar. A second rotating rod is rotatably connected to the inside of the main body, and a rectangular disc is fixedly connected to the top of the second rotating rod. The rectangular disc has equidistant connecting holes circumferentially distributed inside, and these connecting holes are fitted onto the outer wall of the first fixed rod. A rotating shaft is fixedly connected to the top of the rectangular disc, and a connecting sleeve is movably fitted onto the outer wall of the rotating shaft. A placement disc is fixedly connected to the top of the connecting sleeve. When the motor is started, the motor's output shaft drives the first rotating rod to rotate, which in turn drives the notched disc at its top to rotate. The crossbar fixed to the outer wall of the notched disc then rotates in a circular motion, causing the first fixed rod to move synchronously. The first fixed rod engages with and drives the connecting holes on the rectangular disc, causing the rectangular disc on the second rotating rod to rotate. The rotating shaft fixed to the rectangular disc rotates accordingly, thus achieving intermittent rotation, and through the connecting sleeve, driving the placement disc at the top to rotate horizontally.

[0007] Preferably, a spring is movably sleeved on the outer wall of the rotating shaft, and the spring is disposed between the connecting sleeve and the rectangular disk.

[0008] Preferably, a contact plate is fixedly connected to the bottom of the placement tray, and a corrugated plate is fixedly connected to the inner bottom side of the main body. The corrugated plate contacts the contact plate, and the contact plate fixed to the bottom of the placement tray periodically contacts and disengages from the corrugated plate fixed to the bottom of the main body as the tray revolves. Under the buffering and restoring action of the spring, this process forces the entire placement tray to generate periodic longitudinal turbulent motion while revolving horizontally, thereby achieving basic, three-dimensional mixing of all samples in the collection bottles without dead angles.

[0009] Preferably, an auxiliary mechanism is provided on the top of the placement tray. The auxiliary mechanism includes a reciprocating lead screw, which is fixedly connected to the top of the placement tray. A threaded sleeve is threaded to the outer wall of the reciprocating lead screw. A limit rod is fixedly connected to the inner wall of the top of the main body, and the threaded sleeve is movably sleeved on the outer wall of the limit rod.

[0010] Preferably, a fixed sleeve is rotatably connected to the outer wall of the threaded sleeve, a track is fixedly connected to the top of the placement tray, a sliding sleeve is slidably connected to the outer wall of the track, a first connecting rod is hinged between the sliding sleeve and the fixed sleeve, a third rotating rod is rotatably connected to the top of the sliding sleeve, a turntable is fixedly connected to the top of the third rotating rod, and a liquid dispensing bottle is fixedly connected to the top of the turntable. Driven by the revolution of the placement tray, the reciprocating screw fixed to its top rotates synchronously. The threaded sleeve, which is threadedly engaged with the reciprocating screw, cannot rotate under the restriction of the limiting rod, and can only perform precise vertical up-and-down reciprocating motion. The vertical motion of the threaded sleeve is converted into a force that drives the sliding sleeve to perform horizontal radial reciprocating motion along the track through the fixed sleeve and the first connecting rod rotatably connected to it.

[0011] Preferably, a stop bar is fixedly connected to the outer wall of the turntable, and a vertical sleeve is fixedly connected to the top of the placement tray. The vertical sleeve is fitted onto the outer wall of the stop bar. The radial movement of the sliding sleeve further drives the turntable and the liquid collection bottle at its top to move radially through the third rotating rod, so that each liquid collection bottle can receive personalized mixing with adjustable intensity according to its different radial position, especially for enhanced treatment of easily sedimented samples. When the turntable moves radially, it drives the stop bar to move. The stop bar rotates under the restriction of the vertical sleeve, thereby driving the liquid collection bottle at the top of the turntable to rotate through the third rotating rod.

[0012] Preferably, the inside of the liquid filling tank is provided with an automatic liquid replenishment mechanism, which includes a second delivery pipe. The second delivery pipe is fixedly connected to the inner wall of the liquid filling tank and is connected to a first delivery pipe. A third delivery pipe is connected to the bottom of the second delivery pipe.

[0013] Preferably, a piston block is movably connected inside the second delivery pipe. A second fixed rod is fixedly connected to the front of the piston block. A second connecting rod is sleeved on the outer wall of the second fixed rod. The second connecting rod is rotatably connected to the second delivery pipe. A third connecting rod is fixedly connected to the side of the second connecting rod. A float is fixedly connected to the end of the third connecting rod away from the second connecting rod. When the level of sheath fluid in the filling tank drops due to consumption, the float sinks with the fluid level. The sinking of the float pulls the second fixed rod through the third and second connecting rods, causing the piston block to move inside the second delivery pipe, thereby opening the communication channel between the second and third delivery pipes. Under the drive of gravity or system pressure, the external sheath fluid source flows into the bottom of the filling tank sequentially through the inlet pipe, the first delivery pipe, the second delivery pipe, and the third delivery pipe for replenishment. As replenishment proceeds, the liquid level in the filling tank gradually rises, causing the float to float upward. Through the reverse transmission of the linkage mechanism, the piston block is finally pushed to reset, re-closing the connection channel between the second and third delivery pipes, and the replenishment process automatically stops. This purely mechanical feedback control ensures stable liquid level maintenance and avoids pressure surges in the detection liquid path caused by liquid replenishment.

[0014] The present invention, by adopting the above technical solution, can bring the following beneficial effects:

[0015] 1. This flow cytometer, designed for easy fluid replenishment, utilizes a transmission system of a notched disc, a fixed rod, and a rectangular disc to enable intermittent rotation of the placement disc. This allows for the initiation of the next mixing cycle by suddenly applying a shear force from a static state. This instantaneous impact, transitioning from stillness to motion, is often more effective than continuous, gentle vortexing in breaking up cell clumps that are just beginning to aggregate or adhere to the tube walls. It essentially "resets" the sedimentation process repeatedly, dismantling the clumps with maximum force each time. Furthermore, the horizontal rotation, combined with the springs and wave plates, creates periodic longitudinal turbulence of the placement disc, causing the sampling bottle to shake up and down. This generates strong liquid vortices and impacts, thoroughly breaking up cell clumps at the bottom of the tube. This effectively eliminates dead zones present in traditional mixing methods, ensuring that samples, especially large-volume and easily sedimenting samples, maintain a high degree of homogeneity throughout long-term analysis.

[0016] 2. This flow cytometer, designed for easy fluid replenishment, utilizes a reciprocating lead screw that rotates under the drive of the placement disc's revolution. This rotation forces the threaded sleeve to move precisely and periodically in the vertical direction. This linear motion is immediately converted into a force via the first connecting rod, driving the sliding sleeve to move radially along a track. This type of motion enables the sampling bottle to move radially, improving mixing efficiency. Furthermore, the overall motion of the placement disc is combined with the independent mixing action for each sampling bottle. The engagement of the stop lever and the vertical sleeve drives each sampling bottle to rotate independently and powerfully on top of its revolution, further enhancing mixing. This completely disrupts any stable flow field that might form within the liquid, creating a strong "scraping" and "impact" effect on sediment on the tube walls and bottom edges, resulting in a mixing thoroughness far exceeding any single form of mixing.

[0017] 3. This flow cytometer, designed for easy fluid replenishment, employs a float as the liquid level sensing element. A sophisticated linkage mechanism directly converts the drop in liquid level into linear motion of the piston, thus opening the replenishment channel. This purely mechanical feedback control offers high sensitivity and reliability, avoiding potential malfunctions associated with electronic sensors. The replenishment process is continuous and stable, effectively preventing shocks and interference to the core detection fluid flow caused by sudden changes in replenishment pressure, providing a stable fluid environment for obtaining high-precision data. Attached Figure Description

[0018] Figure 1 This is a front view of the structure of the present invention;

[0019] Figure 2 This is a rear view of the structure of the present invention;

[0020] Figure 3 This is a cross-sectional view of the structure of the present invention;

[0021] Figure 4 This is an exploded view of the shaking mechanism of the present invention;

[0022] Figure 5 This is a partial view of the auxiliary structure of the present invention;

[0023] Figure 6 This is an exploded view of the structural auxiliary mechanism of the present invention;

[0024] Figure 7 This is a cross-sectional view of the automatic fluid replenishment mechanism of the present invention;

[0025] Figure 8 This is a partial view of the automatic liquid replenishment mechanism of the present invention.

[0026] In the diagram: 1. Main body; 2. Liquid filling tank; 3. Liquid inlet pipe; 4. First delivery pipe; 5. Shaking mechanism; 511. Motor; 512. First rotating rod; 513. Notched disc; 514. Crossbar; 515. First fixing rod; 516. Second rotating rod; 517. Rotating shaft; 518. Connecting sleeve; 519. Spring; 520. Wave plate; 521. Contact plate; 522. Rectangular disc; 523. Connecting hole; 6. Placement disc; 7. Auxiliary mechanism; 711. Reciprocating mechanism 712. Lead screw; 713. Lead sleeve; 714. Fixing sleeve; 715. Limiting rod; 716. Track; 717. Sliding sleeve; 718. First connecting rod; 719. Third rotating rod; 720. Turntable; 721. Liquid dispensing bottle; 722. Stop bar; 723. Vertical sleeve; 8. Automatic liquid replenishment mechanism; 812. Second delivery pipe; 813. Third delivery pipe; 814. Piston block; 815. Second fixing rod; 816. Second connecting rod; 817. Third connecting rod; 818. Float. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Please see Figure 1-8One embodiment of the present invention is as follows: a flow cytometer for easy fluid replenishment, comprising a body 1, a liquid filling tank 2 fixedly connected to the side of the body 1, an inlet pipe 3 connected to the right side of the liquid filling tank 2, a first delivery pipe 4 provided on the back of the liquid filling tank 2, a shaking mechanism 5 provided inside the body 1, the shaking mechanism 5 including a motor 511, the motor 511 fixedly connected to the bottom of the body 1, the output end of the motor 511 fixedly connected to a first rotating rod 512 via a coupling, the first rotating rod 512 movably passing through the body 1 and extending into it, a notched disc 513 fixedly connected to the top of the first rotating rod 512, and a crossbar 514 fixedly connected to the outer wall of the notched disc 513. A first fixed rod 515 is fixedly connected to the top of the crossbar 514. A second rotating rod 516 is rotatably connected inside the body 1. A rectangular disk 522 is fixedly connected to the top of the second rotating rod 516. The rectangular disk 522 has equidistant connecting holes 523 on its interior. The connecting holes 523 are fitted onto the outer wall of the first fixed rod 515. A rotating shaft 517 is fixedly connected to the top of the rectangular disk 522. A connecting sleeve 518 is movably fitted onto the outer wall of the rotating shaft 517. A placement disk 6 is fixedly connected to the top of the connecting sleeve 518. When the motor 511 is started, the output shaft of the motor 511 drives the first rotating rod 512 to rotate. The first rotating rod 512 drives the notched disk 513 on its top to rotate. The crossbar 514, fixed to the outer wall of the notched disk 513, moves in a circular motion, causing the first fixed rod 515 to move synchronously. The first fixed rod 515 is inserted into and drives the connecting holes 523 on the rectangular disk 522, causing the rectangular disk 522 on the second rotating rod 516 to rotate. The rotating shaft 517, fixed to the rectangular disk 522, rotates intermittently, driving the top placement disk 6 to rotate horizontally via the connecting sleeve 518. A spring 519 is movably sleeved on the outer wall of the rotating shaft 517, positioned between the connecting sleeve 518 and the rectangular disk 522. A contact plate 521 is fixedly connected to the bottom of the placement disk 6, and a corrugated plate 520 is fixedly connected to the inner bottom side of the main body 1. The corrugated plate 520 contacts the contact plate 521. As the placement disk 6 revolves, the contact plate 521 periodically contacts and disengages from the corrugated plate 520 fixed to the bottom of the main body 1. Under the buffering and resetting action of the spring 519, this process forces the entire placement disk 6 to generate periodic longitudinal turbulent motion while revolving horizontally, thereby achieving basic, three-dimensional mixing of samples in all sampling bottles 720 without dead angles.

[0029] Working Principle: The motor 511 is started, and its output shaft drives the first rotating rod 512 to rotate. The first rotating rod 512 drives the notched disk 513 at its top to rotate. The crossbar 514 fixed to the outer wall of the notched disk 513 then rotates in a circular motion, driving the first fixed rod 515 to move synchronously. The first fixed rod 515 engages with and drives the connecting hole 523 on the rectangular disk 522, causing the rectangular disk 522 on the second rotating rod 516 to rotate. The rotating shaft 517 fixed to the rectangular disk 522 rotates accordingly, thus achieving intermittent rotation, and driving the placement disk 6 at the top to rotate horizontally through the connecting sleeve 518. Simultaneously, the contact plate 521 fixed to the bottom of the placement disk 6, as it revolves, periodically contacts and disengages from the wave plate 520 fixed to the bottom of the main body 1. Under the buffering and restoring action of the spring 519, this process forces the entire placement disk 6 to generate periodic longitudinal turbulent motion while revolving horizontally, thereby achieving basic, three-dimensional mixing of samples in all sampling bottles 720 without dead angles.

[0030] Please see Figure 1-8Based on the above embodiments, in another embodiment of the present invention, an auxiliary mechanism 7 is provided on the top of the placement tray 6. The auxiliary mechanism 7 includes a reciprocating screw 711, which is fixedly connected to the top of the placement tray 6. A threaded sleeve 712 is threadedly connected to the outer wall of the reciprocating screw 711. A limiting rod 714 is fixedly connected to the inner wall of the top of the main body 1. The threaded sleeve 712 is movably sleeved on the outer wall of the limiting rod 714. A fixed sleeve 713 is rotatably connected to the outer wall of the threaded sleeve 712. A track 715 is fixedly connected to the top of the placement tray 6. A sliding sleeve 716 is slidably connected to the outer wall of the track 715. A first connecting rod 717 is hinged between the sliding sleeve 716 and the fixed sleeve 713. A third rotating rod 718 is rotatably connected to the top of the sliding sleeve 716. A turntable 719 is fixedly connected to the top of the third rotating rod 718. A liquid dispensing bottle 720 is fixedly connected to the top of the turntable 719. Driven by the revolution of the placement tray 6, the reciprocating screw 711 fixed to its top rotates synchronously. The threaded sleeve 712, which is threadedly engaged with the reciprocating lead screw 711, cannot rotate under the restriction of the limiting rod 714, and can only perform precise vertical up-and-down reciprocating motion. The vertical movement of the threaded sleeve 712 is converted into a force that drives the sliding sleeve 716 to reciprocate horizontally along the track 715 via the fixed sleeve 713 and the first connecting rod 717 rotatably connected to it. A stop rod 721 is fixedly connected to the outer wall of the turntable 719, and a vertical sleeve 722 is fixedly connected to the top of the placement tray 6. The vertical sleeve 722 is fitted onto the outer wall of the stop rod 721. The radial movement of the sliding sleeve 716 further drives the turntable 719 and the liquid collection bottle 720 at its top to move radially via the third rotating rod 718, so that each liquid collection bottle can receive personalized mixing with adjustable intensity according to its radial position, especially for enhanced treatment of easily sedimented samples. When the turntable 719 moves radially, it drives the stop rod 721 to move. The stop rod 721 rotates under the restriction of the vertical sleeve 722, thereby driving the liquid collection bottle 720 at the top of the turntable 719 to rotate via the third rotating rod 718.

[0031] Working principle: Driven by the revolution of the placement disk 6, the reciprocating screw 711 fixed to its top rotates synchronously. The threaded sleeve 712, which is threadedly engaged with the reciprocating screw 711, cannot rotate under the restriction of the limiting rod 714, and can only perform precise vertical up-and-down reciprocating motion. The vertical motion of the threaded sleeve 712 is converted into a force that drives the sliding sleeve 716 to perform horizontal radial reciprocating motion along the track 715 through the fixed sleeve 713 and the first connecting rod 717 rotatably connected to it. The radial movement of the sliding sleeve 716 further drives the turntable 719 and the liquid collection bottle 720 at its top to move radially through the third rotating rod 718, so that each liquid collection bottle can receive personalized mixing with adjustable intensity according to its radial position, especially for enhanced treatment of easily sedimented samples. When the turntable 719 moves radially, it drives the stop rod 721 to move. The stop rod 721 rotates under the restriction of the vertical sleeve 722, thereby driving the liquid collection bottle 720 at the top of the turntable 719 to rotate through the third rotating rod 718.

[0032] Please see Figure 1-8 Based on the above embodiments, in another embodiment of the present invention, an automatic replenishment mechanism 8 is provided inside the liquid filling tank 2. The automatic replenishment mechanism 8 includes a second delivery pipe 812, which is fixedly connected to the inner wall of the liquid filling tank 2. The second delivery pipe 812 is connected to the first delivery pipe 4. A third delivery pipe 813 is connected to the bottom of the second delivery pipe 812. A piston block 814 is movably connected inside the second delivery pipe 812. A second fixing rod 815 is fixedly connected to the front of the piston block 814. A second connecting rod 816 is sleeved on the outer wall of the second fixing rod 815. The second connecting rod 816 is rotatably connected to the second delivery pipe 812. A third connecting rod 817 is fixedly connected to the side of the second connecting rod 816. A float ball 818 is fixedly connected to the end of the third connecting rod 817 away from the second connecting rod 816. When the level of the sheath fluid in the liquid filling tank 2 drops due to consumption, the float ball 818 sinks with the fluid level. The sinking of the float 818 pulls the second fixed rod 815 via the third connecting rod 817 and the second connecting rod 816, causing the piston block 814 to move within the second delivery pipe 812, thereby opening the communication channel between the second delivery pipe 812 and the third delivery pipe 813. Driven by gravity or system pressure, the external sheath fluid source flows sequentially through the inlet pipe 3, the first delivery pipe 4, the second delivery pipe 812, and the third delivery pipe 813 into the bottom of the filling tank 2 for replenishment. As replenishment proceeds, the liquid level in the filling tank 2 gradually rises, causing the float 818 to float upwards. Through the reverse transmission of the linkage mechanism, the piston block 814 is finally pushed back to its original position, re-closing the connection channel between the second delivery pipe 812 and the third delivery pipe 813, and the replenishment process automatically stops. This purely mechanical feedback control ensures stable liquid level maintenance and avoids pressure shocks to the detection liquid path caused by replenishment.

[0033] Working Principle: When the sheath fluid level in the filling tank 2 drops due to consumption, the float 818 sinks with the fluid. The sinking of the float 818 pulls the second fixed rod 815 via the third connecting rod 817 and the second connecting rod 816, causing the piston block 814 to move within the second delivery pipe 812, thereby opening the connection channel between the second delivery pipe 812 and the third delivery pipe 813. Driven by gravity or system pressure, the external sheath fluid source flows sequentially into the bottom of the filling tank 2 through the inlet pipe 3, the first delivery pipe 4, the second delivery pipe 812, and the third delivery pipe 813 to replenish the fluid. As replenishment proceeds, the fluid level in the filling tank 2 gradually rises, causing the float 818 to float upwards. Through the reverse transmission of the linkage mechanism, the piston block 814 is finally pushed to reset, re-closing the connection channel between the second delivery pipe 812 and the third delivery pipe 813, and the replenishment process automatically stops. This purely mechanical feedback control ensures stable fluid level maintenance and avoids pressure shocks to the detection fluid path caused by replenishment.

[0034] This invention provides a flow cytometer that facilitates fluid resuscitation. Many methods and approaches exist for implementing this technical solution; the above description is merely a preferred embodiment of the invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this invention, and these improvements and modifications should also be considered within the scope of protection of this invention. All components not explicitly stated in this embodiment can be implemented using existing technologies.

Claims

1. A flow cytometer for easy fluid resuscitation, comprising a main body (1), characterized in that: The main body (1) is fixedly connected to a liquid filling tank (2) on its side. A liquid inlet pipe (3) is connected to the right side of the liquid filling tank (2). A first delivery pipe (4) is provided on the back of the liquid filling tank (2). The body (1) is equipped with a shaking mechanism (5), which includes a motor (511). The motor (511) is fixedly connected to the bottom of the body (1). The output end of the motor (511) is fixedly connected to a first rotating rod (512) via a coupling. The first rotating rod (512) movably passes through the body (1) and extends into it. A notched disc (513) is fixedly connected to the top of the first rotating rod (512). A crossbar (514) is fixedly connected to the outer wall of the notched disc (513). A second crossbar (514) is fixedly connected to the top of the crossbar (514). A fixed rod (515) is rotatably connected to the inside of the main body (1). A rectangular disk (522) is fixedly connected to the top of the second rotating rod (516). A connecting hole (523) is equidistantly opened in the inside of the rectangular disk (522). The connecting hole (523) is sleeved on the outer wall of the first fixed rod (515). A rotating shaft (517) is fixedly connected to the top of the rectangular disk (522). A connecting sleeve (518) is movably sleeved on the outer wall of the rotating shaft (517). A placement disk (6) is fixedly connected to the top of the connecting sleeve (518).

2. The flow cytometer for easy fluid resuscitation according to claim 1, characterized in that: A spring (519) is movably sleeved on the outer wall of the rotating shaft (517), and the spring (519) is disposed between the connecting sleeve (518) and the rectangular disk (522).

3. A flow cytometer for easy fluid resuscitation according to claim 2, characterized in that: The bottom of the placement tray (6) is fixedly connected to an abutment plate (521), and the inner bottom side of the body (1) is fixedly connected to a wave plate (520), which is in contact with the abutment plate (521).

4. A flow cytometer for easy fluid resuscitation according to claim 3, characterized in that: An auxiliary mechanism (7) is provided on the top of the placement tray (6). The auxiliary mechanism (7) includes a reciprocating screw (711), which is fixedly connected to the top of the placement tray (6). A threaded sleeve (712) is threadedly connected to the outer wall of the reciprocating screw (711). A limiting rod (714) is fixedly connected to the top inner wall of the body (1), and the threaded sleeve (712) is movably sleeved on the outer wall of the limiting rod (714).

5. A flow cytometer for easy fluid resuscitation according to claim 4, characterized in that: The outer wall of the threaded sleeve (712) is rotatably connected to a fixed sleeve (713), the top of the placement tray (6) is fixedly connected to a track (715), the outer wall of the track (715) is slidably connected to a sliding sleeve (716), a first connecting rod (717) is hinged between the sliding sleeve (716) and the fixed sleeve (713), the top of the sliding sleeve (716) is rotatably connected to a third rotating rod (718), the top of the third rotating rod (718) is fixedly connected to a turntable (719), and the top of the turntable (719) is fixedly connected to a liquid collection bottle (720).

6. A flow cytometer for easy fluid resuscitation according to claim 5, characterized in that: A stop bar (721) is fixedly connected to the outer wall of the turntable (719), and a vertical sleeve (722) is fixedly connected to the top of the placement plate (6). The vertical sleeve (722) is fitted onto the outer wall of the stop bar (721).

7. A flow cytometer for easy fluid resuscitation according to claim 6, characterized in that: The liquid filling tank (2) is equipped with an automatic liquid replenishment mechanism (8). The automatic liquid replenishment mechanism (8) includes a second delivery pipe (812). The second delivery pipe (812) is fixedly connected to the inner wall of the liquid filling tank (2). The second delivery pipe (812) is connected to the first delivery pipe (4). The bottom of the second delivery pipe (812) is connected to a third delivery pipe (813).

8. A flow cytometer for easy fluid resuscitation according to claim 7, characterized in that: A piston block (814) is movably connected inside the second conveying pipe (812). A second fixing rod (815) is fixedly connected to the front of the piston block (814). A second connecting rod (816) is sleeved on the outer wall of the second fixing rod (815). The second connecting rod (816) is rotatably connected to the second conveying pipe (812). A third connecting rod (817) is fixedly connected to the side of the second connecting rod (816). A float (818) is fixedly connected to the end of the third connecting rod (817) away from the second connecting rod (816).

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