Preparation method and application of hydrogel loaded with PRP and MXene

Abstract

The invention discloses a preparation method and application of hydrogel loaded with PRP and MXene, and belongs to the technical field of new material preparation. The preparation method comprises the following steps: S1, preparing a hydrogel mold, mixing polyvinyl alcohol with PRP to obtain microneedle glue, and mixing polyvinyl alcohol with MXene to obtain slow-release glue; s2, injecting microneedle glue into the microneedle holes, and covering the groove with polyvinyl alcohol and MXene; s3, standing and forming to obtain the hydrogel. The PRP is stably loaded in a hydrogel network with high crosslinking density, so that the release time of the PRP on the local part of the wound surface is remarkably prolonged, and the oxidation resistance, the antibacterial property and the cell viability regulation and control capability of the gel are enhanced under the synergistic effect of MXene. More importantly, the high hydrophilicity of the MXene promotes rapid gelling of the hydrogel, meanwhile, the mechanical strength and tissue adhesion of the hydrogel are improved, and good adaptive support is provided for complex wounds.

Description

Technical Field

[0001] This invention belongs to the field of new material preparation technology, specifically relating to a method for preparing and applying hydrogels loaded with PRP and MXene. Background Technology

[0002] Skin damage caused by severe trauma, burns, or chronic diseases (such as diabetes) is often accompanied by complex inflammatory responses, restricted angiogenesis, and delayed tissue remodeling, which seriously threatens the patient's quality of life.

[0003] Therefore, developing a multifunctional, bio-friendly material system that can synergistically promote the entire wound healing process has become a research hotspot in the fields of tissue engineering and regenerative medicine.

[0004] Researchers are actively exploring various technologies to enhance wound healing and prevent associated diseases. Recent advancements in biomedical engineering and materials science have led to innovative wound healing methods, such as the utilization of biomaterials and drug delivery systems. Notably, hydrogels and wound dressings are examples of biomaterials that can accelerate tissue regeneration and wound healing. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide a method for preparing and applying a hydrogel loaded with PRP and MXene.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a method for preparing a hydrogel loaded with PRP and MXene, comprising the following steps, S1. Prepare a hydrogel mold, and mix polyvinyl alcohol with PRP to obtain microneedle gel, and mix polyvinyl alcohol with MXene to obtain a slow-release gel; S2. Inject the microneedle adhesive into the microneedle hole, and cover the groove with polyvinyl alcohol and MXene; S3. The hydrogel can be obtained by standing and allowing it to set.

[0007] Furthermore, the volume ratio of PRP to polyvinyl alcohol is 1:1; the volume ratio of MXene to polyvinyl alcohol is 1:100.

[0008] Furthermore, the PRP is prepared by a two-step centrifugation method to obtain the supernatant from plasma.

[0009] Furthermore, the hydrogel is used to promote wound healing.

[0010] Furthermore, the hydrogel exhibits antibacterial properties upon irradiation with near-infrared light.

[0011] The beneficial effects of this invention are as follows: This invention is the first to construct a hydrogel loaded with PRP and MXene, aiming to achieve a multimodal synergistic wound repair strategy. The hydrogel of this invention features zoned loading and sequential action: the upper layer is responsible for surface antibacterial treatment, while the lower layer penetrates deep into the dermis to release healing-promoting factors, achieving precise spatial and temporal separation and synergy of therapeutic functions. The intelligent delivery of the microneedle adhesive: the microneedle array can penetrate necrotic tissue or eschar, delivering therapeutic components directly and painlessly to the dermal layer rich in blood vessels and cells. This is an intelligent solution to the problem of traditional dressings failing to effectively adhere or penetrate moist wounds.

[0012] This invention, by stably loading PRP into a highly cross-linked hydrogel network, not only significantly prolongs its release time at the wound site but also enhances the gel's antioxidant, antibacterial, and cell activity regulation capabilities through the synergistic effect of MXene. More importantly, the high hydrophilicity of MXene promotes rapid gelation of the hydrogel while improving its mechanical strength and tissue adhesion, providing excellent adaptive support for complex wounds.

[0013] Other advantages, objectives, and features of the invention will be set forth in the following description and will be apparent to those skilled in the art in some respects, or may be learned by practice of the invention. The objectives and other advantages of the invention can be realized and obtained through the following description. Attached Figure Description

[0014] To make the objectives, technical solutions, and beneficial effects of this invention clearer, the following figures are provided for illustration: Figure 1 This is a schematic diagram illustrating the hydrogel action of the present invention; Figure 2 A, 2B, and 2C are the PRP sampling flowcharts of this invention; Figure 3 HE staining image from in vivo experiments of this invention; Figure 4 This is a trend diagram of the sustained-release effect of the hydrogel in this invention; Figure 5 A, 5B: Escherichia coli non-toxicity test diagram of the present invention; Figure 6 A, 6B: Non-toxic test diagrams of Staphylococcus aureus in this invention; Figure 7 This is a schematic diagram of a hydrogel mold; Figure 8 This is a schematic diagram of microneedle electron scanning.

[0015] The following are marked in the attached diagram: 1. Base 1; 2. Groove 2; 3. Micro-pinhole 3. Detailed Implementation

[0016] like Figures 1-8 As shown, this invention discloses a method for preparing and applying a hydrogel loaded with PRP and MXene. The hydrogel mold used in this invention is as follows... Figure 7 As shown, it includes a disc-shaped base 1, a cube-shaped groove 2 in the center of the base 1, and several cone-shaped micro-pinholes 3 in the groove 2.

[0017] As shown in 2A and 2B, a two-step centrifugation method was used to collect PRP from plasma, taking the supernatant from each step, and finally taking... Figure 2 The lower half of C obtains the final PCP. Includes the following steps: S1. Mix PRP and polyvinyl alcohol at a volume ratio of 1:1 to obtain microneedle adhesive. Then mix MXene and polyvinyl alcohol at a volume ratio of 1:100 to obtain slow-release adhesive. S2. Inject the microneedle adhesive into the microneedle hole 3 to fill the microneedle hole 3, and let it stand for 30 seconds before injecting the slow-release adhesive into the groove 2 to form the shape. S3. After standing and setting, a hydrogel loaded with PRP and MXene can be obtained.

[0018] like Figure 1 As shown, when the hydrogel prepared by this invention is applied to the wound surface, the microneedle array can penetrate necrotic tissue or scab and deliver the therapeutic components directly and painlessly to the dermal layer rich in blood vessels and cells. The upper layer is responsible for surface antibacterial treatment, while the lower layer penetrates deep into the dermis to release healing-promoting factors, thus achieving precise separation and synergy of therapeutic functions in space and time. test: ① HE staining.

[0019] Blank group, MXene group, PRP group and hydrogel group were prepared respectively; HE staining was performed on heart, liver, spleen, lung and kidney.

[0020] Figure 3 As shown, in both the normal and treatment groups, the HE staining results of the heart, liver, spleen, lungs and kidneys in each group showed normal structures with no specific structural damage or changes, proving that the composite hydrogel has good biocompatibility in animals and that the hydrogel is non-toxic.

[0021] Figure 4 This is a schematic diagram of the action time of the hydrogel of the present invention. After more than 15 days of action, its efficiency can still reach 90%, while traditional PRP needs to be replaced after only 24 hours when applied to the wound. ② Perform E. coli testing.

[0022] After co-culturing with untreated bacteria in a culture medium for a period of time, the bacteria were centrifuged, digested, and fixed with pentylene glycol. The morphology observed during transmission electron microscopy was then analyzed, as shown in the image. Figure 5As shown in Figure A, the morphology is short rod-shaped, with a continuous and intact cell membrane, uniform and dense cytoplasm, and a smooth and regular overall outline without structural damage.

[0023] An equal volume of bacterial culture medium containing MXene discrete solution was added, and the bacteria were co-cultured for a period of time. After centrifugation and digestion, the bacteria were fixed with pentylene glycol, and the morphology observed during transmission electron microscopy was analyzed. (The image shows...) Figure 5 As shown in B, the cell membrane in this morphology is discontinuous and partially dissolved, with leakage of cytoplasmic contents, and the overall outline is shrunken and distorted, with severe structural damage.

[0024] The hydrogel of this invention effectively promotes wound healing. ③ Conduct the golden grape ball test.

[0025] After co-culturing with untreated bacteria in a culture medium for a period of time, the bacteria were centrifuged, digested, and fixed with pentylene glycol. The morphology observed during transmission electron microscopy was then analyzed, as shown in the image. Figure 6 As shown in Figure A, the morphology is spherical, with a continuous and intact cell membrane, uniform and dense cytoplasm, and a smooth and regular overall outline without structural damage.

[0026] An equal volume of bacterial culture medium containing MXene discrete solution was added, and the bacteria were co-cultured for a period of time. After centrifugation and digestion, the bacteria were fixed with pentylene glycol, and the morphology observed during transmission electron microscopy was analyzed. (The image shows...) Figure 6 As shown in B, the cell membrane is discontinuous and partially dissolved, the cytoplasm is uneven, the contents are leaking out, the overall outline is wrinkled and distorted, the edges are regular, and the structure is severely damaged.

[0027] The hydrogel of this invention effectively promotes wound healing. In summary, the hydrogel loaded with PRP and MXene in this invention exhibits excellent angiogenesis promotion, inflammation regulation, and epithelial regeneration effects in a mouse full-thickness skin defect model, demonstrating its broad application prospects as a synergistic carrier of "bioactivity + intelligent response" in regenerative medicine. It provides a novel technical path for chronic wound repair and offers theoretical support and experimental basis for the translational application of MXene in tissue engineering. Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made to it in form and detail without departing from the scope defined by the claims of the present invention.

Claims

1. A method for preparing a hydrogel loaded with PRP and MXene, characterized in that: Includes the following steps, S1. Prepare a hydrogel mold, and mix polyvinyl alcohol with PRP to obtain microneedle gel, and mix polyvinyl alcohol with MXene to obtain a slow-release gel; S2. Inject the microneedle adhesive into the microneedle hole, and cover the groove with polyvinyl alcohol and MXene; S3. The hydrogel can be obtained by standing and allowing it to set.

2. The method for preparing a hydrogel loaded with PRP and MXene according to claim 1, characterized in that: The volume ratio of PRP to polyvinyl alcohol is 1:1; the volume ratio of MXene to polyvinyl alcohol is 1:

100.

3. The method for preparing a hydrogel loaded with PRP and MXene according to claim 1, characterized in that: The PRP is prepared by centrifuging plasma in two steps, and the supernatant is obtained from each step.

4. The hydrogel according to any one of claims 1-3, used to promote wound healing.

5. The hydrogel according to any one of claims 1-3, after being irradiated with near-infrared light, volatilizes its antibacterial properties.

Images