Cuttage method for disporum cantoniense
By treating the root segments of *Bambusa textilis* with magnetic treatment agents and bud-promoting gels, combined with magnetic adsorption technology, the problem of difficult budding of *Bambusa textilis* root segments was solved, achieving efficient propagation and high survival rate.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- XIAN BOTANICAL GARDEN SHAANXI PROV
- Filing Date
- 2025-12-31
- Publication Date
- 2026-05-01
AI Technical Summary
Bamboo root cuttings have difficulty sprouting, resulting in low propagation efficiency.
The root segments of *Bambusa textilis* were treated with a magnetic treatment agent and a bud-promoting gel, and then magnetic adsorption technology was used to promote the sprouting of *Bambusa textilis* root cuttings.
This improved the propagation efficiency and survival rate of bamboo cuttings, resulting in cuttings with well-developed root systems.
Abstract
Description
A method for propagating mahogany by cuttings Technical Field
[0001] This invention relates to the field of cutting propagation technology for Bambusa textilis, and more particularly to a method for cutting propagation of Bambusa textilis. Background Technology
[0002] Disporum cantoniense, a perennial herbaceous plant belonging to the genus Disporum in the family Liliaceae, grows upright and is commonly found in forest edges, undergrowth, and grassy slopes. It flowers in spring, from May to August, with flower colors ranging from white, pale green, and yellow to purple, exhibiting a wide color spectrum. Its elegant form makes it suitable as a flowering ground cover or border plant. Its roots and rhizomes also have medicinal uses. Wild Disporum cantoniense has a low natural propagation rate, and with increasing market demand for medicinal and landscaping applications, its resources are gradually decreasing. Therefore, there is an urgent need for the introduction, cultivation, and standardized propagation of Disporum cantoniense.
[0003] Research on tissue culture of *Dracaena sanderiana* is limited; propagation is mainly based on seed and cuttings. Seed propagation is simple, produces seedlings with well-developed root systems and robust growth, but the germination rate of *Dracaena sanderiana* seeds is low, resulting in slow propagation. Cutting propagation is simple, fast, and maintains the plant's excellent quality. *Dracaena sanderiana* is generally propagated by stem cuttings, requiring the selection of current-year stem segments with nodes. However, *Dracaena sanderiana* has a relatively small above-ground biomass, resulting in a limited number of cuttings per plant. Furthermore, the root systems of cuttings are underdeveloped, leading to low transplant survival rates and overall low propagation efficiency. Root cuttings, on the other hand, use the plant's root system as cuttings. Root segments have a strong rooting ability, producing well-developed root systems after cutting, thus improving transplant survival rates. Since *Dracaena sanderiana* has a well-developed root system, a larger number of cuttings can be obtained per plant. Therefore, root cuttings can significantly improve the propagation efficiency of *Dracaena sanderiana*. However, root segments of plants lack buds, making it difficult for cuttings to sprout. Furthermore, the strong rooting ability of root segments leads to a significant depletion of nutrients in the cuttings during the rooting process, further inhibiting sprouting. This results in root segment cuttings often producing only roots without sprouting, failing to yield viable seedlings. Therefore, a new method for propagating *Dracaena sanderiana* by cuttings is urgently needed to address the problem of low propagation efficiency. Summary of the Invention
[0004] Therefore, the purpose of this invention is to provide a cutting propagation method for *Bambusa textilis*, which solves the problem of difficult sprouting from root cuttings and improves the propagation efficiency of *Bambusa textilis*.
[0005] The present invention solves the above-mentioned technical problems through the following technical means:
[0006] A method for propagating Bamboo Sapium sebiferum by cuttings includes the following steps:
[0007] (1) Pretreatment of plants: Select healthy 2-year-old bamboo plants as mother plants, apply magnetic treatment agent to the soil around the roots of the mother plants and let them grow naturally for 2-3 days to complete the pretreatment of plants.
[0008] (2) Taking cuttings: Dig up the pretreated mother plant, wash the roots, and cut the root segments with a diameter of ≥3mm as cuttings. The length of the cuttings should be 6-7cm. When cutting, cut the upper end flat and the lower end at an angle.
[0009] (3) Pretreatment of cuttings: First soak the cuttings in 0.2wt% potassium permanganate for 10-15 minutes, then soak them in magnetic treatment agent for 5 minutes, then suspend the magnet at 50cm above the cuttings for magnetic attraction treatment for 20-30 minutes, and after treatment, evenly apply a layer of bud-promoting gel to the top of the cuttings to obtain the treated cuttings.
[0010] (4) Cutting and management: Prepare a substrate by mixing perlite, vermiculite and river sand. After disinfecting the substrate, water it thoroughly. Then insert the lower end of the treated cuttings into the substrate to complete the cutting. After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃. For the first 1-7 days of cutting, use a magnet to magnetically attract the cuttings for 2 hours every day. After 7 days, stop the magnetic attraction treatment.
[0011] (5) Transplanting: Transplant to the field 58-60 days after cutting.
[0012] Furthermore, in step (1), the amount of magnetic treatment agent applied is 50-60g / plant.
[0013] Furthermore, the preparation method of the magnetic treatment agent is as follows:
[0014] S1: Add nano-iron oxide and polyvinylpyrrolidone to phosphate buffer solution with pH=7-7.2, and sonicate in an ice-water bath for 5 min to obtain a magnetic solution;
[0015] S2: Soybean lecithin and cholesterol are dissolved in ethanol and evaporated on a rotary evaporator at 45-50℃ until the solvent is completely evaporated to form a thin film. Then, a magnetic solution is added and the mixture is placed in an ice-water bath and ultrasonically treated for 25-30 minutes. The supernatant is removed by centrifugation to obtain a liposome suspension. Mannitol is added to the liposome suspension and mixed evenly. The mixture is then freeze-dried to obtain magnetic nanoliposomes.
[0016] S3: Mix magnetic nanoliposomes with water at a mass ratio of 1:1000 and stir until homogeneous to obtain a magnetic treatment agent.
[0017] Furthermore, in step S1, the mass ratio of nano-ferric oxide, polyvinylpyrrolidone, and phosphate buffer is (0.2-0.3):1:50.
[0018] Furthermore, the particle size of the nano-iron oxide is 20 nm.
[0019] Furthermore, in step S2, the mass ratio of soybean lecithin, cholesterol, and magnetic solution is (3-4):1:50.
[0020] Furthermore, the centrifugation speed is 10000-12000 rpm, and the centrifugation time is 5 min; the freeze-drying temperature is -20℃, and the freeze-drying time is 6-8 h.
[0021] Furthermore, the magnetic nanoliposomes have a particle size of 50 nm.
[0022] Furthermore, the magnets used in step (3) are ferrite magnets with a magnetic field strength of 600-800 Gs.
[0023] Furthermore, the magnets used in step (4) are all ferrite magnets, wherein the magnetic field strength of the magnet used in the 1st-2nd days is 600Gs, the magnetic field strength of the magnet used in the 3rd-5th days is 400Gs, and the magnetic field strength of the magnet used in the 6th-7th days is 200Gs.
[0024] Furthermore, the preparation method of the sprouting gel is as follows:
[0025] Add 6-BA and IBA to a 3-3.5 wt% sodium alginate solution and stir until homogeneous to obtain a germination-promoting gel.
[0026] Furthermore, the concentration of 6-BA in the bud-promoting gel is 1 g / kg, and the concentration of IBA is 0.5 g / kg.
[0027] Furthermore, the amount of the sprouting gel used is 0.1g / plant.
[0028] Furthermore, in step (4), the cutting depth is 3-4cm and the plant spacing is 5cm×5cm.
[0029] Plant roots have a strong rooting ability. Using root segments of *Bambusa textilis* for propagation can increase the number of cuttings and produce well-developed root systems. However, *Bambusa textilis* root segments lack buds, making sprouting difficult. Therefore, this invention treats *Bambusa textilis* to improve the sprouting rate of root segment cuttings. This invention treats the roots of *Bambusa textilis* with a magnetic treatment agent and a sprouting gel, and magnetically treats the cut cuttings to promote sprouting from the root segment cuttings.
[0030] 6-BA and IBA are plant growth regulators that can regulate plant cell proliferation and differentiation. High concentrations of 6-BA and low concentrations of IBA can be used together to promote the germination of root segments of *Bambusa textilis*. In this invention, 6-BA and IBA are added to sodium alginate to prepare a germination-promoting gel to promote germination at the upper end of the cuttings. However, after the *Bambusa textilis* root segments are removed from the plant, they do not have an upward transpiration pull. 6-BA and IBA enter the cutting and diffuse towards the lower end of the cutting under the action of gravity. They cannot remain at the upper end of the cutting to continuously stimulate and promote germination. Instead, they promote the production and growth of new roots at the lower end of the cutting and competitively consume the nutrients stored in the root segments, further reducing the germination rate. Therefore, this invention prepares a magnetic treatment agent, the main components of which are magnetic nanoliposomes prepared from lecithin, cholesterol, nano-ferric oxide, and polyvinylpyrrolidone. The liposomes formed from lecithin and cholesterol have a phospholipid bilayer structure similar to that of plant cell membranes, enabling them to move within the plant. Simultaneously, they provide a stable environment for the magnetic nano-ferric oxide, preventing it from prematurely losing its magnetism due to the acidic environment of the soil and the roots of *Bambusa textilis*. Polyvinylpyrrolidone can chelate plant growth regulators, preventing their loss. This invention first applies the magnetic treatment agent to the root soil of the *Bambusa textilis* mother plant. Utilizing the active absorption and transport mechanism of the intact *Bambusa textilis* root system, the magnetic treatment agent is effectively accumulated within the mother plant's root system, ensuring that the subsequently cut root segments have the necessary material basis. After cutting the root segments into cuttings, a magnet is used to magnetically attract the cuttings. The magnetic attraction of the nano-ferric oxide by the magnet promotes the directional enrichment of the magnetic treatment agent dispersed within the cuttings at the upper end of the cuttings. After the application of the bud-promoting gel, the polyvinylpyrrolidone in the magnetic treatment agent chelates and enriches 6-BA and IBA at the upper end of the cuttings, providing a continuous and sufficient amount of hormone signals to the root cuttings without buds, thereby effectively inducing bud differentiation and germination, solving the problem of difficult bud sprouting in root cuttings. In addition, after the magnetic treatment agent has been acting in the root cuttings of Thymocarpus for a long time, the nano-iron oxide in it reacts into iron ions in the acidic environment inside the plant and is absorbed by the root cuttings, promoting the growth of Thymocarpus buds and leaves and improving the success rate of Thymocarpus root cuttings.
[0031] In addition, the present invention can also activate the physiological activity of cutting cells by utilizing the magnetic field effect of the magnet during the magnetic treatment process, thereby enhancing their stress resistance and overall survival rate after cutting.
[0032] Beneficial effects:
[0033] This invention uses root segments of *Bambusa textilis* as cuttings, utilizing the strong rooting ability of *Bambusa textilis* roots to obtain cuttings with well-developed root systems. Simultaneously, by using a magnetic treatment agent and a bud-promoting gel, combined with magnetic attraction, the plant growth regulators in the bud-promoting gel are concentrated at the upper end of the cuttings, promoting budding and resulting in cuttings with well-developed roots and buds, thus improving the efficiency of *Bambusa textilis* propagation by cuttings. Detailed Implementation
[0034] The present invention will be described in detail below with reference to specific embodiments:
[0035] Example 1: Preparation of the treatment agent
[0036] Preparation of magnetic treatment agent:
[0037] S1: Add 0.2g of 20nm nano-iron oxide and 1g of polyvinylpyrrolidone to 50g of pH=7 phosphate buffer solution, and sonicate in an ice-water bath at 150W for 5min to obtain a magnetic solution.
[0038] S2: Weigh 4g of soybean lecithin and 1g of cholesterol and dissolve them in 10g of anhydrous ethanol. Evaporate the solution in a rotary evaporator at 45℃ until the solvent is completely evaporated to form a thin film. Then add 50g of magnetic solution and place the film in an ice-water bath. Sonicate the film at 150W for 25min. Centrifuge the film at 12000rpm for 5min to remove the supernatant and obtain a liposome suspension. Add 0.5g of mannitol to the liposome suspension and mix well. Freeze-dry the film at -20℃ for 6h to obtain magnetic nanoliposomes with a particle size of 50nm.
[0039] S3: Mix magnetic nanoliposomes with water at a mass ratio of 1:1000 and stir until homogeneous to obtain a magnetic treatment agent.
[0040] Preparation of sprouting gel:
[0041] 6-BA and IBA were added to a 3.5 wt% sodium alginate solution and stirred until homogeneous to obtain a germination-promoting gel with a 6-BA concentration of 1 g / Kg and an IBA concentration of 0.5 g / Kg.
[0042] Example 2: Preparation of the treatment agent
[0043] Preparation of magnetic treatment agent:
[0044] S1: Add 0.3g of 20nm nano-iron oxide and 1g of polyvinylpyrrolidone to 50g of phosphate buffer solution with pH=7.2, and sonicate in an ice-water bath at 150W for 5min to obtain a magnetic solution.
[0045] S2: Weigh 3g of soybean lecithin and 1g of cholesterol and dissolve them in 10g of anhydrous ethanol. Evaporate the solution in a rotary evaporator at 50℃ until the solvent is completely evaporated to form a thin film. Then add 50g of magnetic solution and place the film in an ice-water bath. Sonicate the film at 150W for 30min. Centrifuge the film at 10000rpm for 5min to remove the supernatant and obtain a liposome suspension. Add 0.5g of mannitol to the liposome suspension and mix well. Freeze-dry the film at -20℃ for 8h to obtain magnetic nanoliposomes with a particle size of 50nm.
[0046] S3: Mix magnetic nanoliposomes with water at a mass ratio of 1:1000 and stir until homogeneous to obtain a magnetic treatment agent.
[0047] Preparation of sprouting gel:
[0048] 6-BA and IBA were added to a 3 wt% sodium alginate solution and stirred until homogeneous to obtain a germination-promoting gel with a 6-BA concentration of 1 g / Kg and an IBA concentration of 0.5 g / Kg.
[0049] Comparative Example 1:
[0050] Compared with the magnetic treatment agent of Example 1, the only difference is that the treatment agent prepared in Comparative Example 1 does not contain nano-iron oxide. The specific preparation method is as follows:
[0051] Preparation of the treatment agent:
[0052] S1: Add 1g of polyvinylpyrrolidone to 50g of phosphate buffer solution with pH=7, and sonicate in an ice-water bath at 150W for 5min to obtain a mixed solution.
[0053] S1: Weigh 4g of soybean lecithin and 1g of cholesterol and dissolve them in 10g of anhydrous ethanol. Evaporate the solution in a rotary evaporator at 45℃ until the solvent is completely evaporated to form a thin film. Add 50g of the mixed solution and place it in an ice-water bath. Sonicate the solution at 150W for 25min. Centrifuge at 12000rpm for 5min to remove the supernatant and obtain a liposome suspension. Add 0.5g of mannitol to the liposome suspension and mix well. Freeze-dry the solution at -20℃ for 6h to obtain nanoliposomes with a particle size of 50nm.
[0054] S3: Mix nanoliposomes and water at a mass ratio of 1:1000 and stir until homogeneous to obtain a magnetic treatment agent.
[0055] Comparative Example 2:
[0056] Compared with Example 1, the only difference is that the magnetic treatment agent prepared in Comparative Example 2 does not contain polyvinylpyrrolidone. The specific preparation method is as follows:
[0057] Preparation of magnetic treatment agent:
[0058] S1: Add 0.2g of 20nm nano-iron oxide to 50g of pH=7 phosphate buffer solution, and sonicate in an ice-water bath at 150W for 5min to obtain a magnetic solution.
[0059] S2: Weigh 4g of soybean lecithin and 1g of cholesterol and dissolve them in 10g of anhydrous ethanol. Evaporate the solution in a rotary evaporator at 45℃ until the solvent is completely evaporated to form a thin film. Then add 50g of magnetic solution and place the film in an ice-water bath. Sonicate the film at 150W for 25min. Centrifuge the film at 12000rpm for 5min to remove the supernatant and obtain a liposome suspension. Add 0.5g of mannitol to the liposome suspension and mix well. Freeze-dry the film at -20℃ for 6h to obtain magnetic nanoliposomes with a particle size of 50nm.
[0060] S3: Mix magnetic nanoliposomes with water at a mass ratio of 1:1000 and stir until homogeneous to obtain a magnetic treatment agent.
[0061] Example 3: Bamboo cuttings
[0062] (1) Pretreatment of plants: Select healthy 2-year-old bamboo plants as mother plants, apply magnetic treatment agent to the soil around the roots of the mother plants at a dosage of 50g / plant, and let them grow naturally for 3 days to complete the pretreatment of plants.
[0063] (2) Taking cuttings: Dig up the pretreated mother plant, wash the roots, and cut the root segments with a diameter of ≥3mm as cuttings. The cuttings should be 7cm long, with the upper end cut flat and the lower end cut at an angle.
[0064] (3) Pretreatment of cuttings: First, soak the cuttings in 0.2wt% potassium permanganate for 15 minutes, then soak them in magnetic treatment agent for 5 minutes, and then use a magnet with a magnetic field strength of 800Gs to suspend the cuttings at 50cm above the cuttings for magnetic attraction treatment for 20 minutes. After the treatment, apply 0.1g of bud-promoting gel evenly to the top of the cuttings to obtain the treated cuttings.
[0065] (4) Cutting and management: Prepare a substrate by mixing perlite, vermiculite and river sand in a volume ratio of 1:1:2. After disinfecting the substrate, water it thoroughly. Then, insert the lower end of the treated cuttings into the substrate at a depth of 4cm and a spacing of 5cm×5cm to complete the cutting. After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃. For the first 1-7 days of cutting, use a magnet to magnetically attract the cuttings for 2 hours every day. The magnetic field strength of the magnet used on the first 1-2 days is 600Gs, the magnetic field strength of the magnet used on the 3-5 days is 400Gs, and the magnetic field strength of the magnet used on the 6-7 days is 200Gs. After 7 days, no more magnetic attraction is required.
[0066] (5) Transplanting: Transplant to the field 60 days after cutting.
[0067] Comparative Example 3:
[0068] Compared with Example 3, the only difference is that Example 3 did not treat the Bambusa textilis plants. The specific operation is as follows:
[0069] (1) Taking cuttings: Select a healthy 2-year-old Chinese bamboo as the mother plant. Dig up the Chinese bamboo mother plant, wash the roots, and cut root segments with a diameter of ≥3mm as cuttings. The cuttings should be 7cm long. When cutting, make a flat cut at the top and a slanted cut at the bottom.
[0070] (2) Pretreatment of cuttings: First, soak the cuttings in 0.2wt% potassium permanganate for 15 minutes, then soak them in magnetic treatment agent for 5 minutes, and then use a magnet with a magnetic field strength of 800Gs to suspend the cuttings at 50cm above the cuttings for magnetic attraction treatment for 20 minutes. After the treatment, apply 0.1g of bud-promoting gel evenly to the top of the cuttings to obtain the treated cuttings.
[0071] (3) Cutting and management: Prepare a substrate by mixing perlite, vermiculite and river sand in a volume ratio of 1:1:2. After disinfecting the substrate, water it thoroughly. Then, insert the lower end of the treated cuttings into the substrate at a depth of 4cm and a spacing of 5cm×5cm to complete the cutting. After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃. For the first 1-7 days of cutting, use a magnet to magnetically attract the cuttings for 2 hours every day. The magnetic field strength of the magnet used on the first 1-2 days is 600Gs, the magnetic field strength of the magnet used on the 3-5 days is 400Gs, and the magnetic field strength of the magnet used on the 6-7 days is 200Gs. After 7 days, no more magnetic attraction is required.
[0072] (4) Transplanting: Transplant to the field 60 days after cutting.
[0073] Comparative Example 4:
[0074] Compared with Example 3, the only difference in Comparative Example 4 is that the cuttings were not soaked in a magnetic treatment agent during pretreatment. The specific operation is as follows:
[0075] (1) Pretreatment of plants: Select healthy 2-year-old bamboo plants as mother plants, apply magnetic treatment agent to the soil around the roots of the mother plants at a dosage of 50g / plant, and let them grow naturally for 3 days to complete the pretreatment of plants.
[0076] (2) Taking cuttings: Dig up the pretreated mother plant, wash the roots, and cut the root segments with a diameter of ≥3mm as cuttings. The cuttings should be 7cm long, with the upper end cut flat and the lower end cut at an angle.
[0077] (3) Pretreatment of cuttings: First, soak the cuttings in 0.2wt% potassium permanganate for 15 minutes, then use a magnet with a magnetic field strength of 800Gs to suspend the cuttings at 50cm above the cuttings for 20 minutes. After treatment, apply 0.1g of bud-promoting gel evenly to the top of the cuttings to obtain the treated cuttings.
[0078] Steps (4) and (5) are the same as in Example 3.
[0079] Comparative Example 5:
[0080] Compared with Example 3, the only difference in Comparative Example 5 is that the amount of magnetic treatment agent used in the pretreatment of the plants was increased. The specific operation is as follows:
[0081] (1) Pretreatment of plants: Select healthy 2-year-old bamboo plants as mother plants, apply magnetic treatment agent to the soil around the roots of the mother plants at a dosage of 100g / plant, and let them grow naturally for 3 days to complete the pretreatment of plants.
[0082] Steps (2)-(5) are the same as in Example 3.
[0083] Comparative Example 6:
[0084] Compared with Example 3, the only difference is that Comparative Example 6 does not use magnetic attraction during the cutting and propagation process. The specific operation is as follows:
[0085] Steps (1)-(3) are the same as in Example 3;
[0086] (4) Cuttings and maintenance: Prepare a substrate by mixing perlite, vermiculite and river sand in a volume ratio of 1:1:2. After disinfecting the substrate, water it thoroughly. Then, insert the lower end of the treated cuttings into the substrate at a depth of 4cm and a spacing of 5cm×5cm to complete the cutting. After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃.
[0087] (5) Transplanting: Transplant to the field 60 days after cutting.
[0088] Comparative Example 7:
[0089] Compared with Example 3, the only difference is that the magnetic field strength of the magnet used in Comparative Example 7 for the magnetic attraction treatment during cutting propagation was 600 Gs. The specific operation is as follows:
[0090] Steps (1)-(3) are the same as in Example 3;
[0091] (4) Cuttings and care: Prepare a substrate by mixing perlite, vermiculite and river sand in a volume ratio of 1:1:2. After disinfecting the substrate, water it thoroughly. Then, insert the lower end of the treated cuttings into the substrate at a depth of 4cm and a spacing of 5cm×5cm to complete the cutting. After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃. For the first 1-7 days of cutting, use a magnet with a magnetic field strength of 600Gs to magnetically attract the cuttings for 2 hours every day. After 7 days, stop the magnetic attraction treatment.
[0092] (5) Transplanting: Transplant to the field 60 days after cutting.
[0093] Comparative Example 8:
[0094] Compared with Example 3, the only difference is that the magnetic field strength of the magnet used in Comparative Example 8 for the magnetic attraction treatment during cutting propagation was 400 Gs. The specific operation is as follows:
[0095] Steps (1)-(3) are the same as in Example 3;
[0096] (4) Cuttings and care: Prepare a substrate by mixing perlite, vermiculite and river sand in a volume ratio of 1:1:2. After disinfecting the substrate, water it thoroughly. Then, insert the lower end of the treated cuttings into the substrate at a depth of 4cm and a spacing of 5cm×5cm to complete the cutting. After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃. For the first 1-7 days of cutting, use a magnet with a magnetic field strength of 400Gs to magnetically attract the cuttings for 2 hours every day. After 7 days, stop the magnetic attraction treatment.
[0097] (5) Transplanting: Transplant to the field 60 days after cutting.
[0098] Comparative Example 9:
[0099] Compared with Example 3, the only difference is that the magnetic field strength of the magnet used in Comparative Example 9 for the magnetic attraction treatment during cutting propagation was 200 Gs. The specific operation is as follows:
[0100] Steps (1)-(3) are the same as in Example 3;
[0101] (4) Cuttings and care: Prepare a substrate by mixing perlite, vermiculite and river sand in a volume ratio of 1:1:2. After disinfecting the substrate, water it thoroughly. Then, insert the lower end of the treated cuttings into the substrate at a depth of 4cm and a spacing of 5cm×5cm to complete the cutting. After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃. For the first 1-7 days of cutting, use a magnet with a magnetic field strength of 200Gs to magnetically attract the cuttings for 2 hours every day. After 7 days, stop the magnetic attraction treatment.
[0102] (5) Transplanting: Transplant to the field 60 days after cutting.
[0103] Comparative Example 10:
[0104] Compared with Example 3, the only difference in Comparative Example 10 is that the magnetic treatment time during cutting propagation was extended until the cuttings sprouted. The specific operation is as follows:
[0105] Steps (1)-(3) are the same as in Example 3;
[0106] (4) Cutting and management: Prepare a substrate by mixing perlite, vermiculite and river sand in a volume ratio of 1:1:2. After disinfecting the substrate, water it thoroughly. Then, insert the lower end of the treated cuttings into the substrate at a depth of 4cm and a spacing of 5cm×5cm to complete the cutting. After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃. From the time the cuttings sprout, use a magnet to treat the cuttings for 2 hours every day. The magnetic field strength of the magnet used on the first and second days is 600Gs, and the magnetic field strength of the magnet used on the third to fifth days is 400Gs. From the sixth day onwards, use a magnet with a magnetic field strength of 200Gs for 2 hours every day until the cuttings sprout. After sprouting, stop the magnetic treatment.
[0107] (5) Transplanting: Transplant to the field 60 days after cutting.
[0108] Blank group:
[0109] (1) Taking cuttings: Select healthy 2-year-old Chinese bamboo as the mother plant, dig out and wash the roots, cut the root segments with a diameter ≥ 3mm as cuttings, the cutting length is 7cm, and when cutting, cut the upper end flat and the lower end obliquely.
[0110] (2) Pretreatment of cuttings: Soak cuttings in 0.2wt% potassium permanganate for 15 minutes, and apply 0.1g of bud-promoting gel evenly to the upper part of the cuttings to obtain the treated cuttings;
[0111] (3) Cuttings and maintenance: Prepare a substrate by mixing perlite, vermiculite and river sand in a volume ratio of 1:1:2. After disinfecting the substrate, water it thoroughly. Then, insert the lower end of the treated cuttings into the substrate at a depth of 4cm and a spacing of 5cm×5cm to complete the cutting. After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃.
[0112] (4) Transplanting: Transplant to the field 60 days after cutting.
[0113] experiment:
[0114] Following the above method, two-year-old Bambusa textilis plants with similar growth were selected from the same batch as the mother plants for the experiment, with 100 cuttings in each group.
[0115] Experiment on cuttings of Taraxacum mongolicum:
[0116] (1) Plant pretreatment: Apply magnetic treatment agent to the soil around the roots of the mother plant at a dosage of 50g / plant and let it grow naturally for 3 days to complete the plant pretreatment.
[0117] (2) Taking cuttings: Dig up the pretreated mother plant, wash the roots, and cut the root segments with a diameter of 4mm as cuttings. The cuttings should be 7cm long, with the upper end cut flat and the lower end cut at an angle.
[0118] (3) Pretreatment of cuttings: First, soak the cuttings in 0.2wt% potassium permanganate for 15 minutes, then soak them in magnetic treatment agent for 5 minutes, and then use a magnet with a magnetic field strength of 800Gs to suspend the cuttings at 50cm above the cuttings for magnetic attraction treatment for 20 minutes. After the treatment, apply 0.1g of bud-promoting gel evenly to the top of the cuttings to obtain the treated cuttings.
[0119] (4) Cuttings and management: Prepare a substrate by mixing perlite, vermiculite and river sand in a volume ratio of 1:1:2. After disinfecting the substrate, water it thoroughly. Then, insert the lower end of the treated cuttings into the substrate at a depth of 4cm and a spacing of 5cm×5cm to complete the cuttings. After cuttings, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃. For the first 1-7 days of cuttings, use a magnet to magnetically attract the cuttings for 2 hours every day. The magnetic field strength of the magnet used on the first 1-2 days is 600Gs, the magnetic field strength of the magnet used on the 3-5 days is 400Gs, and the magnetic field strength of the magnet used on the 6-7 days is 200Gs. After 7 days, no more magnetic attraction is required.
[0120] (5) Transplanting: Transplant to the field 60 days after cutting.
[0121] 1. Experimental group 1 and control groups 1-10 all used the sprouting gel prepared in Example 1.
[0122] 2. Experimental group 1 and control groups 3-10 all used the magnetic treatment agent prepared in Example 1; control group 1 used the treatment agent prepared in Comparative Example 1; control group 2 used the magnetic treatment agent prepared in Comparative Example 2.
[0123] 3. Experimental group 1 and control groups 1-2 were propagated by cuttings of *Dendrobium nobile* using the above method. Control group 1 was not subjected to magnetic attraction treatment. Control groups 3-10 were propagated by cuttings of *Dendrobium nobile* using the method described in control group 3-10.
[0124] 4. The blank group was propagated by cuttings of *Bambusa textilis* using the same method as the blank group, and the bud-promoting gel prepared in Example 1 was used.
[0125] After cutting, the time of first bud emergence (≥0.5cm) in each group of cuttings was recorded. On day 35 after cutting, 10 plants were randomly selected to count the average number of roots (counting the number of new roots ≥1cm) and the average root length (counting the root length of new roots ≥1cm). The results are shown in Table 1. On day 60 after cutting, the survival rate and average bud height (counting the height of buds ≥0.5cm) of each group of cuttings were counted. The results are shown in Table 1.
[0126] Table 1
[0127] Sprouting Time | Average Number of Roots / cm | Average Root Length / cm | Average Bud Height / cm | Cutting Survival Rate / % | Experimental Group 1 | 12d | 5.8 | 6.2 | 5.6 | 9 | 3 | | Control Group 1 | 16d | 5.8 | 6.1 | 3.7 | 7 | | Control Group 2 | 19 | 5.8 | 6.2 | 3.2 | 6 | 9 | | Control Group 3 | 18d | 5.7 | 6.0 | 3.6 | 7 | | Control Group 4 | 15d | 5.8 | 6.1 | 4.2 | 8 | | Control Group 5 | 25d | 3.4 | 4.1 | 1.4 | 4 | | Control Group 6 | 17d | 5.6 | 6.3 | 3.8 | 7 | | Control Group 7 | 12d | 3.9 | 4.8 | 5.5 | 6 | | Control Group 8 | 13d | 4.8 | 5.7 | 4.9 | 8 | | Control Group 9 | 16d | 5.6 | 6.0 | 4.3 | 8 | | Control Group 10 | 12d | 4.4 | 3.2 | 5.6 | 7 | | Blank Group | 20d | 5.8 | 6.0 | 2.7 | 6 | 4 surface
[0128] Analysis of the data in Table 1 shows that:
[0129] 1. Experimental group 1 used the cutting method of the present invention to propagate bamboo cuttings. The root cuttings sprouted quickly, developed a well-developed root system, and thus had a high survival rate, resulting in a large number of effective seedlings and improving propagation efficiency. Moreover, the transplant survival rate was high, indicating that the cutting treatment method of the present invention does not affect the activity of the cuttings.
[0130] 2. Compared with experimental group 1, the treatment agent used in control group 1 did not contain nano-iron oxide. Therefore, the magnetic attraction of the magnet could not concentrate the treatment agent at the upper end of the cutting and distribute it evenly inside the cutting. During the cutting, only a small amount of 6-BA and IBA could be chelated and fixed at the upper end of the cutting, which could not provide sufficient hormone stimulation to the upper end of the cutting, resulting in slower budding time, poor bud growth, and low cutting survival rate. The magnetic treatment agent used in control group 2 did not contain polyvinylpyrrolidone, which could not chelate and fix 6-BA and IBA. After the cutting, the 6-BA and IBA in the bud-promoting gel continued to diffuse to the lower end of the cutting, which could not provide sufficient hormone stimulation to the upper end of the cutting, resulting in difficulty in budding and a decrease in cutting survival rate.
[0131] 3. Compared with experimental group 1, control group 3 did not treat the *Dracaena sanderiana* plants with magnetic treatment agents, but only treated the cuttings. The cuttings were detached root segments of *Dracaena sanderiana*, and their absorption capacity was significantly reduced compared to a growing, intact *Dracaena sanderiana* plant. Therefore, the content of magnetic treatment agents in the cuttings after soaking was low, resulting in a weaker chelation and enrichment effect on 6-BA and IBA in the bud-promoting gel. Consequently, 6-BA and IBA in the bud-promoting gel entered the upper part of the cutting and gradually diffused to the lower part, failing to maintain a high concentration of hormones at the upper part of the cutting to induce budding. This led to a longer budding time, lower budding height, and some cuttings dying before budding, resulting in a low survival rate. Control group 4 did not use magnetic treatment agents during the pretreatment of the cuttings. In the case of cuttings treated with magnetic sap, the magnetic treatment agent inside the cuttings was carried out by the sap during the cutting and disinfection process and could not be replenished, resulting in a decrease in the content of magnetic treatment agent inside the cuttings. Therefore, there was less magnetic treatment agent at the upper end of the cuttings after magnetic treatment, which reduced the chelation effect of 6-BA and IBA. The insufficient growth hormone led to a longer sprouting time and a lower survival rate of the cuttings. In control group 5, the amount of magnetic treatment agent used to treat the mother plants of *Dendrobium nobile* was increased. After the *Dendrobium nobile* root segments absorbed more magnetic treatment agent, the content of iron tetroxide and polyvinylpyrrolidone inside increased, which may have a certain toxic effect on the root and stem cells of *Dendrobium nobile*, resulting in reduced activity of the obtained cuttings, slower rooting and sprouting, and therefore a low survival rate of the cuttings.
[0132] 4. Compared with experimental group 1, control group 6 did not use magnetic attraction during the post-cutting management process. Although the magnetic treatment agent was concentrated at the upper part of the cuttings during pre-treatment, it gradually diffused to the lower part of the cuttings under gravity after the magnet was removed. This prevented the 6-BA and IBA in the bud-promoting gel from accumulating at the upper part of the cuttings, resulting in slower bud emergence and lower cutting survival rate. Control group 7 used a 600Gs magnet for magnetic attraction during cutting management, and control group 8 used a 400Gs magnet. The magnetic treatment agent accumulated in large quantities at the upper part of the cuttings for a long time. The polyvinylpyrrolidone in the magnetic treatment agent also had a negative effect on the rooting auxin. The chelating agent has a chelating effect, fixing auxin at the upper end of the cutting. Insufficient auxin at the lower end of the cutting affects rooting and leads to a decrease in the survival rate of the cuttings. In control group 9, a 200Gs magnet was used for magnetic attraction during the cutting management. The lower magnetic field strength resulted in a weaker magnetic attraction of the magnetic treatment agent, leading to less accumulation of the magnetic treatment agent at the upper end of the cutting. Consequently, it was impossible to chelate and fix a large amount of 6-BA and IBA at the upper end of the cutting, resulting in poor budding and a decrease in the survival rate of the cuttings. In control group 10, the magnetic attraction treatment time was extended. The continuous magnetic attraction treatment enriched the magnetic adsorbent at the upper end of the cutting for a long time. The auxin that promotes rooting was fixed at the upper end of the cutting for a long time, affecting the rooting of the cutting and leading to a decrease in the survival rate of the cuttings.
[0133] 5. The blank group was treated with conventional cuttings. After the root cuttings of Phyllostachys edulis were taken, the buds sprouted slowly and the buds grew slowly. Many cuttings only grew roots and did not grow buds, and gradually died in the later stage of the cuttings, resulting in a low survival rate and low propagation efficiency.
[0134] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.
Claims
1. A method for propagating Bambusa textilis by cuttings, characterized in that, The method is as follows: (1) Plant pretreatment: Select a healthy 2-year-old Chinese tallow tree as the mother plant, apply a magnetic treatment agent to the soil around the roots of the mother plant and let it grow naturally for 2-3 days to complete the plant pretreatment; (2) Cuttings: Dig out the pretreated mother plant, wash the roots, and cut root segments with a diameter ≥3mm as cuttings. The length of the cuttings is 6-7cm. When cutting, cut the upper end flat and the lower end oblique; (3) Cuttings pretreatment: Soak the cuttings in 0.2wt% potassium permanganate for 10-15 minutes, then soak them in the magnetic treatment agent for 5 minutes, and then apply the magnetic treatment agent. (3) Attaching the iron to the upper end of the cutting at 50cm for 20-30 minutes, and then applying a layer of bud-promoting gel evenly to the upper end of the cutting to obtain the treated cutting; (4) Cutting and management: Prepare a substrate by mixing perlite, vermiculite and river sand. After disinfecting the substrate, water it thoroughly and then insert the lower end of the treated cutting into the substrate to complete the cutting; After cutting, maintain an ambient humidity of 75-80% and a temperature of 25℃±1℃; For the first 1-7 days of cutting, use a magnet to apply magnetic treatment to the cutting for 2 hours every day. After 7 days, stop applying magnetic treatment; (5) Transplanting: Transplant to the field 58-60 days after the cutting is completed.
2. The method for propagating *Dendrobium nobile* by cuttings as described in claim 1, characterized in that... In step (1), the amount of magnetic treatment agent applied is 50-60g / plant.
3. The method for propagating *Dendrobium nobile* by cuttings as described in claim 2, characterized in that... The preparation method of the magnetic treatment agent is as follows: S1: Add nano-iron oxide and polyvinylpyrrolidone to phosphate buffer solution with pH=7-7.2, and sonicate in an ice-water bath for 5 min to obtain a magnetic solution; S2: Dissolve soybean lecithin and cholesterol in ethanol, and evaporate on a rotary evaporator at 45-50℃ until the solvent is completely evaporated to form a thin film. Then add the magnetic solution, place in an ice-water bath and sonicate for 25-30 min. Centrifuge to remove the supernatant to obtain a liposome suspension. Add mannitol to the liposome suspension and mix evenly. Freeze-dry to obtain magnetic nanoliposomes; S3: Mix the magnetic nanoliposomes and water at a mass ratio of 1:1000 and stir evenly to obtain the magnetic treatment agent.
4. The method for propagating *Dendrobium nobile* by cuttings as described in claim 3, characterized in that... In step S1, the mass ratio of nano-ferric oxide, polyvinylpyrrolidone, and phosphate buffer is (0.2-0.3):1:
50.
5. The method for propagating *Dendrobium nobile* by cuttings as described in claim 4, characterized in that... In step S2, the mass ratio of soybean lecithin, cholesterol, and magnetic solution is (3-4):1:
50.
6. The method for propagating *Dendrobium nobile* by cuttings as described in claim 5, characterized in that... The magnets used in step (3) are ferrite magnets with a mean magnetic field strength of 600-800 Gs.
7. The method for propagating *Dendrobium nobile* by cuttings as described in claim 6, characterized in that... The magnets used in step (4) are all ferrite magnets. The magnetic field strength of the magnets used in the first 1-2 days is 600 Gs, the magnetic field strength of the magnets used in the third 3-5 days is 400 Gs, and the magnetic field strength of the magnets used in the sixth 6-7 days is 200 Gs.
8. The method for propagating *Dendrobium nobile* by cuttings as described in claim 7, characterized in that... The method for preparing the bud-promoting gel is as follows: 6-BA and IBA are added to a 3-3.5 wt% sodium alginate solution and stirred until homogeneous to obtain the bud-promoting gel.
9. The method for propagating *Dendrobium nobile* by cuttings as described in claim 8, characterized in that... The bud-promoting gel contains 1 g / kg of 6-BA and 0.5 g / kg of IBA.
10. The method for propagating *Dendrobium nobile* by cuttings as described in claim 9, characterized in that... In step (4), the cutting depth is 3-4cm and the spacing between plants is 5cm×5cm.