Method for preparing high-purity pinocembrin through high-speed counter-current chromatography separation

Abstract

The invention relates to a method for preparing high-purity pinocembrin by high-speed counter-current chromatography separation, which comprises the following steps: taking propolis extract as a raw material, fully shaking a solvent system, standing for phase separation, and separately collecting an upper phase and a lower phase; the propolis extract is dissolved in an upper phase, the upper phase serves as a stationary phase, a lower phase serves as a mobile phase, high-speed counter-current chromatography is used for separation and purification, a mixed solution of the pinobufagin and the mobile phase is obtained, the mobile phase is removed, and the pinobufagin is obtained. According to the method, the high-speed counter-current chromatography technology is utilized for the first time to separate and purify the pinocembrin with the purity larger than 98% from the propolis, and the method has the advantages of no sample loss, no pollution, high efficiency, large-scale preparation, solvent recycling and the like; reagents of a solvent system adopted by the method can be recycled and reused, so that the method has a good environment-friendly function.