Method for rapidly detecting microorganisms based on third-generation sequencing

By constructing a pre-computational gene cluster framework and a direct mapping method, the problem of slow data analysis speed in third-generation sequencing was solved, enabling efficient and low-resource-consumption microbial species detection.

CN121963876APending Publication Date: 2026-05-01GUANGDONG HONG KONG MACAO GREATER BAY AREA PRECISION MEDICINE RESEARCH INSTITUTE (GUANGZHOU)
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGDONG HONG KONG MACAO GREATER BAY AREA PRECISION MEDICINE RESEARCH INSTITUTE (GUANGZHOU)
Filing Date
2025-12-22
Publication Date
2026-05-01

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Abstract

The invention provides a method for rapidly detecting microbial species, and particularly, the method comprises the following steps: constructing a double-level database and a corresponding gene cluster; constructing a reference matrix X based on the database and the gene cluster; comparing to-be-detected third-generation sequencing data with the gene cluster to generate equivalence classes, and constructing a counting matrix Y based on the equivalence classes and the gene cluster; based on the counting matrix Y and the reference matrix X, determining the gene abundance in the to-be-detected sample; and determining microbial species in the sample to be detected based on the gene abundance. The rapid pathogen detection method provided by the invention is high in analysis speed, fully utilizes the advantages of the three-generation sequencing data, and can more directly obtain the microorganism composition information of the sample; the method is low in computing resource demand, easy to deploy and high in precision detection capability.
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