Pharmaceutical composition for relieving physical fatigue and preparation method and application thereof

Abstract

The invention discloses a pharmaceutical composition for relieving physical fatigue as well as a preparation method and application thereof. The pharmaceutical composition is prepared from the following raw material medicines in parts by weight: 1-3 parts of cervus elaphus linnaeus, 1.6-3 parts of ginseng, 2-6 parts of fructus lycii, 2-6 parts of schisandra chinensis and 1-3 parts of tortoise shells. According to the preparation method disclosed by the invention, an extraction process of decocting the tortoise shells firstly and adding the ginseng and the like later is adopted, and a spray drying and 85% ethanol granulation technology is combined, so that active ingredients are effectively reserved. Pharmacodynamic experiment results show that the composition can significantly prolong the loaded swimming time of mice, improve the liver glycogen reserve and reduce the levels of blood lactic acid and serum urea nitrogen, and the effect is significantly superior to that of simple superposition of each single medicinal material. The traditional Chinese medicine composition is scientific in formula, remarkable in synergistic interaction, stable in process, high in safety and suitable for conditioning and relieving physical fatigue for a long time.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical technology, and in particular to a pharmaceutical composition for relieving physical fatigue and its application. Background Technology

[0002] With the accelerated pace of modern life and increasing work pressure, chronic fatigue syndrome and physical fatigue are becoming increasingly prevalent among younger people. Physical fatigue not only manifests as muscle soreness and energy depletion, but also involves multiple pathophysiological mechanisms such as mitochondrial dysfunction, oxidative stress damage, and neuroendocrine dysregulation. Currently, products for relieving physical fatigue are mainly divided into two categories: chemical drugs and traditional Chinese medicine or natural products.

[0003] While chemical drugs (such as amphetamines and modafinil) can temporarily increase central nervous system excitability, they are often accompanied by dependence, drug tolerance, and cardiovascular side effects, making them unsuitable for long-term or daily conditioning in sub-healthy individuals. Existing anti-fatigue products on the market, primarily based on traditional Chinese medicine or natural products, mostly follow the traditional approach of "tonifying qi and strengthening yang" or "nourishing yin and replenishing essence," resulting in relatively fixed and highly homogenized formulations. These products generally suffer from the following technical deficiencies: First, their mechanisms of action are relatively singular, mostly focusing only on replenishing energy substrates or clearing lactic acid, making it difficult to synergistically intervene on multiple targets related to "energy metabolism—oxidative stress—neuroprotection"; second, the bioavailability of active ingredients is low, as traditional decoction or crude extraction processes destroy the synergistic ratio of key active ingredients; and third, there is a lack of compatibility optimization based on modern evidence-based medicine, leading to slow onset of action and significant individual differences in efficacy.

[0004] With the development of modern nutrition and molecular pharmacology, the industry has gradually recognized that physical fatigue is the result of multi-system interactions. Traditional single-mode approaches relying solely on "tonification" or "stimulation" are insufficient to meet modern consumers' demand for precise, efficient, safe, and restorative anti-fatigue products. Therefore, based on modern fatigue mechanism research, developing a drug composition with clearly defined components, synergistic mechanisms, and comprehensive intervention across multiple dimensions—energy replenishment, mitochondrial function repair, antioxidant defense, and metabolic homeostasis regulation—is of significant clinical and market value in filling the technological gaps in the depth of efficacy and safety of existing products. Summary of the Invention

[0005] This invention provides a pharmaceutical composition for relieving physical fatigue with clearly defined components, scientific formulation, and synergistic effects, as well as a method for its preparation. Based on the principle of "tonifying both Yin and Yang and mutually generating essence and Qi," this composition significantly enhances its anti-fatigue effect by intervening in energy metabolism and oxidative stress at multiple targets.

[0006] In a first aspect, the present invention provides a pharmaceutical composition for relieving physical fatigue, comprising the following raw materials in the indicated weight ratios: 1-3 parts of deer antler, 1.6-3 parts of ginseng, 2-6 parts of wolfberry, 2-6 parts of schisandra, and 1-3 parts of tortoise shell.

[0007] Preferably, the above-mentioned pharmaceutical composition for relieving physical fatigue is composed of the following raw materials in the indicated weight ratios: 1 part deer antler, 1.6 parts ginseng, 4 parts wolfberry, 4 parts schisandra, and 3 parts tortoise shell.

[0008] Preferably, the above-mentioned pharmaceutical composition is an oral solid dosage form.

[0009] Preferably, the above-mentioned oral solid dosage form is a tablet, granule, or capsule.

[0010] Secondly, the present invention provides a method for preparing the above-mentioned pharmaceutical composition for relieving physical fatigue, comprising the following steps: S1. Powder and sterilize the elk antler, and set aside. S2. Take the tortoise shell, add 6-10 times the amount of water as the medicinal material, and decoct for 1-2 hours to obtain the tortoise shell decoction; S3. Add ginseng, wolfberry and schisandra to the tortoise shell decoction in step (2), and continue to add 6-10 times the amount of water as the medicinal materials. Extract three times, each time for 1-2 hours, filter, and combine the filtrates. S4. Concentrate the filtrate from step S3 to a relative density of 1.05-1.10, and spray dry to obtain a dry powder. S5. The dry powder from step S4 is added to fine powder of deer antler, mixed well, and pharmaceutically acceptable excipients are added to prepare a pharmaceutical composition.

[0011] Preferably, step S5 specifically includes: mixing fine powder of deer antler and dry extract powder with white sugar powder, using 85% edible alcohol as a wetting agent to make a soft material, sieving it into particles through a 16-mesh sieve, and drying it at 50~60℃ to obtain a powder with a moisture content ≤7.0%.

[0012] Further, take the above-mentioned dried powder with a moisture content ≤7.0%, add magnesium stearate, mix well, compress into tablets to obtain tablets.

[0013] Alternatively, take the above-mentioned powder with a moisture content of ≤7.0% after drying, fill it into capsules, and obtain capsules.

[0014] Thirdly, the present invention provides the use of the above-described pharmaceutical composition for relieving physical fatigue or the pharmaceutical composition for relieving physical fatigue obtained by the above-described method in the preparation of products for relieving physical fatigue.

[0015] The pharmaceutical composition for relieving physical fatigue provided by this invention is a pure traditional Chinese medicine preparation. It is formulated to address the core pathogenesis of physical fatigue in modern people caused by overwork and depletion of vital energy—"deficiency of kidney essence as the root cause, and deficiency of both qi and yin as the manifestation." Traditional Chinese medicine believes that "the kidney stores essence, governs bones, produces marrow, and is the organ of strength," meaning that physical strength is closely related to the abundance or deficiency of kidney essence. If kidney essence is insufficient, the marrow sea is empty, leading to soreness and weakness in the lower back and knees, and an inability to endure exertion; if vital energy is deficient, there is a lack of propulsion, and general fatigue. This invention follows the principle of "essence and blood sharing the same origin, and yin and yang mutually rooted," using the treatment method of tonifying the kidney and replenishing essence, nourishing qi and yin, and harmonizing the heart and kidneys.

[0016] The pharmaceutical composition for relieving physical fatigue provided by this invention has the function of relieving physical fatigue. It is based on the classic prescription "Gui Lu Er Xian Jiao" recorded in "Yi Bian" written by Wang Sancai in the Ming Dynasty. Through screening of traditional Chinese medicine prescriptions and commonly used Chinese medicines, combined with scientific experiments on clinical medication rules, Chinese medicine compatibility, health care functions and safety, the composition of the formula was determined. It uses deer antler combined with tortoise shell, ginseng and other medicinal materials to achieve the effect of relieving physical fatigue.

[0017] The principal ingredients in this formula are deer antler and ginseng. Deer antler is sweet, salty, and warm in nature; it enters the liver and kidney meridians. It can tonify kidney yang, replenish essence and blood, and strengthen tendons and bones. The *Compendium of Materia Medica* states that it "generates essence and replenishes marrow, nourishes blood and benefits yin, strengthens tendons and bones, and treats all kinds of deficiency, tinnitus, blurred vision, dizziness, and chronic diarrhea." It is effective in nourishing blood, replenishing essence and marrow, tonifying the kidneys and strengthening tendons, and is particularly beneficial for treating deficiency syndromes such as insufficient essence and blood, weakness of tendons and bones, and slow walking. Ginseng is sweet and slightly bitter, and enters the spleen, lung, heart, and kidney meridians. It has the effects of greatly replenishing vital energy, replenishing qi and generating fluids, replenishing qi and generating blood, calming the mind and improving intelligence, and prolonging life. When these two are combined, kidney essence is replenished and vital energy is supplemented, serving as the principal ingredients to address the root causes of physical weakness from the two fundamental levels of "essence" and "qi."

[0018] Tortoise shell is used as an auxiliary ingredient; its taste is salty and sweet, and it is slightly cold. It enters the liver, kidney, and heart meridians; it has the effects of nourishing yin and suppressing yang, tonifying the kidneys and strengthening bones, and nourishing blood and replenishing the heart. It is used for symptoms such as yin deficiency with tidal fever, night sweats, dizziness, and forgetfulness due to heart deficiency. Its cold nature can counteract the warming and drying properties of deer antler and ginseng, making the entire formula warm without being drying and nourishing without being hot; at the same time, it works synergistically with wolfberry to enhance the effect of nourishing kidney yin, achieving the principle of "seeking yang within yin and yin within yang" in the formula.

[0019] Goji berries and schisandra berries are used as adjuvant herbs. Goji berries are sweet and neutral in nature, entering the liver and kidney meridians; they nourish the liver and kidneys, benefit essence and improve eyesight. They are used for symptoms such as weakness and deficiency of essence, soreness and weakness of the lower back and knees, dizziness and tinnitus. Schisandra berries have astringent and consolidating effects, replenish qi and generate fluids, tonify the kidneys and calm the mind. They are used for chronic cough and asthma, nocturnal emission, frequent urination, chronic diarrhea, spontaneous sweating and night sweats, thirst due to fluid depletion, internal heat and thirst, palpitations and insomnia. Schisandra berries are sour and sweet, warm in nature, and enter the lung, heart, and kidney meridians. The Tang Dynasty's *Xinxiu Bencao* records: "The skin and flesh of schisandra are sweet and sour, the kernel is bitter and pungent, and has a salty taste," hence the name "five-flavor berry." It was first listed as a superior-grade Chinese medicine in the *Shennong Bencao Jing*, possessing nourishing and strengthening power, with high medicinal value and effects of strengthening the body. Both are used as adjuvant herbs, which can enhance the tonifying effect of the principal herb, and also prevent the dissipation of essence and qi through the astringent and consolidating properties of acid, while also taking into account symptoms such as insomnia and night sweats that accompany fatigue.

[0020] The above five herbs, with deer antler and tortoise shell representing yin and yang respectively, one active and one passive, work together to regulate the fundamental balance of yin and yang; ginseng greatly replenishes vital energy, schisandra fruit astringes dissipated energy, and wolfberry nourishes tangible essence. The combined effects of these herbs are mutually beneficial, replenishing both yin and yang, generating essence and qi, being warming without being drying, and astringent while replenishing, thus achieving the effects of replenishing essence and marrow, nourishing qi and yin, and strengthening muscles and bones, fundamentally relieving physical fatigue.

[0021] This invention is not a simple combination of medicinal ingredients, but rather, based on the molecular mechanisms of modern fatigue, it constructs a multi-target synergistic intervention network encompassing "energy metabolism—mitochondrial function—oxidative stress—neuroendocrine": 1. Synergistically regulate energy metabolism and replenish ATP reserves Red deer antler is rich in amino acids, polypeptides and growth factors, which can activate the AMPK (adenosine monophosphate activated protein kinase) signaling pathway, promote glucose uptake and fatty acid oxidation, and enhance the efficiency of ATP synthesis in skeletal muscle mitochondria.

[0022] Ginsenosides Rb1 and Rg1 in ginseng can upregulate the expression of PGC-1α (peroxisome proliferator-activated receptor gamma coactivator 1α), promote mitochondrial biosynthesis, and inhibit lactate dehydrogenase activity, thereby reducing lactate accumulation.

[0023] Lycium barbarum polysaccharides in wolfberries can enhance succinate dehydrogenase activity and improve the efficiency of the tricarboxylic acid cycle.

[0024] Synergistic effect: The four components work together to form a positive regulation of the entire chain of "substrate supply - mitochondrial oxidation - energy output", which is significantly better than that of a single component.

[0025] 2. Multi-target antioxidant effect, reducing oxidative stress damage Physical fatigue is accompanied by the generation of large amounts of reactive oxygen species (ROS), leading to cell membrane lipid peroxidation and mitochondrial dysfunction.

[0026] The lignans in Schisandra chinensis (such as schisandrin B) are potent antioxidants that can activate the Nrf2 (nuclear factor E2-related factor 2) pathway and upregulate the activity of endogenous antioxidant enzymes such as SOD (superoxide dismutase) and GSH-Px (glutathione peroxidase).

[0027] The collagen peptides and trace elements (selenium and zinc) in deer antler and tortoise shell can synergistically scavenge free radicals and protect the integrity of skeletal muscle cell membranes.

[0028] Synergistic effect: Schisandra chinensis provides "initial" antioxidant defense, while ginseng, wolfberry, and deer antler provide "continuous" free radical scavenging, forming an antioxidant cascade effect.

[0029] 3. Regulates the hypothalamus-pituitary-adrenal axis and improves the central mechanism of fatigue. Chronic fatigue often involves HPA axis dysfunction, manifested as abnormal cortisol rhythm.

[0030] Ginsenosides have a bidirectional regulatory effect on the HPA axis, which can reduce abnormally elevated cortisol levels under stress and enhance adrenal cortex reserve function.

[0031] Schisandra chinensis can enhance the inhibitory process of the central nervous system, improve sleep quality, and promote fatigue recovery.

[0032] Synergistic effect: Through the combination of "ginseng invigorating the central nervous system + schisandra calming and astringing + tortoise shell nourishing yin and suppressing yang", homeostatic regulation of the neuroendocrine system is achieved.

[0033] 4. Improve bioavailability and process synergy The preparation process of this invention (decocting tortoise shell first, adding ginseng and other ingredients later, spray drying, and granulation with 85% alcohol) enhances the synergistic effect in the following ways: First decoct the tortoise shell: fully extract the colloids, amino acids, and calcium to form a colloidal protective system, which helps to encapsulate the active ingredients of other herbs and reduce the damage caused by stomach acid.

[0034] Add ginseng and other ingredients later: This avoids prolonged high-temperature hydrolysis of heat-sensitive components such as ginsenosides, thus preserving their activity.

[0035] Spray drying: Instant drying technology preserves volatile and heat-sensitive components, and the resulting dry powder has good dispersibility.

[0036] Granulation with 85% alcohol: selectively enriches isopolar saponins, flavonoids and lignans, improving the dissolution rate of active ingredients.

[0037] Compared with the prior art, the technical effects of the present invention are as follows: 1. Scientific formulation: Following the TCM theory of "supplementing Yin and Yang and generating essence and Qi", the formula is rigorous, warm but not drying, and suitable for long-term conditioning.

[0038] 2. Synergistic effect: Modern pharmacological verification shows that each component has a multi-target synergistic effect in energy metabolism, anti-oxidation and neuroendocrine regulation, and the anti-fatigue effect is significantly better than that of a single component or simple superposition.

[0039] 3. Process optimization: By controlling key processes such as "decocting before adding, spray drying, and granulation with specific concentrations of alcohol", the active ingredients are preserved to the maximum extent, improving product stability and bioavailability.

[0040] 4. High safety: The entire formula is made from food and medicine or traditional tonics, without chemical stimulants, and has no dependence or obvious toxic side effects. Attached Figure Description

[0041] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0042] Figure 1 The production process flow diagram of the pharmaceutical composition product for relieving physical fatigue provided by the present invention is shown. Detailed Implementation

[0043] To enable those skilled in the art to better understand the technical solutions of the present invention, the present invention will be further described in detail below with reference to embodiments and accompanying drawings. Obviously, the described embodiments are merely some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0044] Unless otherwise specified, all experimental materials used in the following examples were purchased from conventional biochemical reagent stores. Unless otherwise specified, all experimental methods used in the following examples are conventional methods.

[0045] Example 1: Preparation of a pharmaceutical composition for relieving physical fatigue 1. Weigh the following raw materials: 1g deer antler, 1.6g ginseng, 4g wolfberry, 4g schisandra, and 3g tortoise shell.

[0046] 2. Powder and sterilize the antler powder (5kGy 60Co sterilization) for later use; take the tortoise shell, add 8 times the amount of water as the medicinal material, and decoct for 1.5 hours; then add ginseng, wolfberry, and schisandra, and continue to add 8 times the amount of water as the medicinal material, extract three times, 1.5 hours each time, filter, combine the filtrates, concentrate the decoction filtrate to a relative density of 1.05-1.10 (measured at 60℃), spray dry (inlet air temperature 150~165℃, outlet air temperature 80~95℃), add the fine powder of antler powder and white sugar powder, mix well, add 85% edible alcohol to make a soft material, granulate through a 16-mesh sieve, dry at 50~60℃ (moisture content ≤7.0%), granulate through an 18-mesh sieve, add magnesium stearate, mix well, and compress into tablets (0.8g / tablet). Example

[0047] 1. Weigh the following raw materials: 3g deer antler, 3g ginseng, 6g wolfberry, 6g schisandra, and 3g tortoise shell.

[0048] 2. Powder and sterilize the antler powder (5kGy 60Co sterilization) for later use; take the tortoise shell, add 6 times the amount of water as the medicinal material, and decoct for 1 hour; then add ginseng, wolfberry, and schisandra, and continue to add 6 times the amount of water as the medicinal material, extract three times, 1 hour each time, filter, combine the filtrates, concentrate the decoction filtrate to a relative density of 1.05-1.10 (measured at 60℃), spray dry (inlet air temperature 150~165℃, outlet air temperature 80~95℃), add the fine powder of antler powder and white sugar powder, mix well, add 85% edible alcohol to make a soft material, granulate through a 16-mesh sieve, dry at 50~60℃ (moisture content ≤7.0%), granulate through an 18-mesh sieve, add magnesium stearate, mix well, and compress into tablets (0.8g / tablet). Example

[0049] 1. Weigh the following raw materials: 2g deer antler, 2g ginseng, 2g wolfberry, 2g schisandra, and 1g tortoise shell.

[0050] 2. Powder and sterilize the antler powder (5kGy 60Co sterilization) for later use; take the tortoise shell, add 10 times the amount of water as the medicinal material, and decoct for 2 hours; then add ginseng, wolfberry, and schisandra, and continue to add 10 times the amount of water as the medicinal material, extract three times, 2 hours each time, filter, combine the filtrates, concentrate the decoction filtrate to a relative density of 1.05-1.10 (measured at 60℃), spray dry (inlet air temperature 150~165℃, outlet air temperature 80~95℃), add the fine powder of antler powder and white sugar powder, mix well, add 85% edible alcohol to make a soft material, granulate through a 16-mesh sieve, dry at 50~60℃ (moisture content ≤7.0%), granulate through an 18-mesh sieve, and fill into capsules to obtain capsules.

[0051] Experiment Example 1: Weighted Swimming Experiment 1. Testing Principle The improvement of exercise endurance is the most direct manifestation of enhanced anti-fatigue ability. The length of swimming time can reflect the degree of exercise fatigue in animals.

[0052] 2. Instruments and Equipment Swimming tank (about 50 cm × 50 cm × 40 cm in size), electronic balance, lead sheet.

[0053] 3. Experimental Methods 3.1 The experimental animals used purebred mice, adult mice, with a body weight of 18 - 22 g.

[0054] 3.2 Dose Grouping and Administration Time of Test Samples The experiment was set up with three dose groups and one negative control group. One of the dose groups was 10 times the recommended human dose, and the other two dose groups were set. The administration time of the test sample was 30 days.

[0055] 3.3 Experimental Procedures 30 minutes after the last administration of the test sample, mice with a 5% body weight lead sheet loaded at the root of the tail were placed in the swimming tank to swim. The water depth was not less than 30 cm, the water temperature was 25°C ± 1.0°C, and the time from the start of swimming until the death of the mice was recorded, that is, the weight-bearing swimming time of the mice.

[0056] 4. Data Processing and Result Judgment The swimming time was measurement data, and analysis of variance was used. First, a homogeneity of variance test was performed according to the procedure of analysis of variance. If the variances were homogeneous, the F value was calculated. If the F value < F0.05, the conclusion was that there was no significant difference between the means of each group; if the F value ≥ F0.05 and P ≤ 0.05, pairwise comparisons of the means between multiple experimental groups and one control group were performed for statistics; for non-normal or non-homogeneous variance data, appropriate variable transformations were carried out. After meeting the requirements of normality or homogeneity of variance, the transformed data were used for statistics; if the purpose of normality or homogeneity of variance was still not achieved after variable transformation, the rank sum test was used for statistics. If the weight-bearing swimming time of the test sample group was significantly longer than that of the control group and the difference was significant, the experimental result could be judged as positive.

[0057] 5. Results Table 1 Influence of the composition prepared in Example 1 on the weight-bearing swimming time of mice (X ± SD)

[0058] *: Significantly different compared with the 0 g / kgBW group Table 2 Influence of the composition prepared in Example 2 on the weight-bearing swimming time of mice (X ± SD)

[0059] Table 3. Effect of the composition prepared in Example 3 on the weight-bearing swimming time of mice (X±SD)

[0060] As shown in Tables 1-3, after oral administration of different doses of this product to mice for 31 days, the composition prepared in Example 1 showed significant differences. Compared with the 0 g / kg BW group, the 2.4 g / kg BW group showed a significant difference in the duration of weight-bearing swimming (P < 0.05), making it the optimal combination.

[0061] Experiment Example 2: Validation of Synergistic Effect To verify the synergistic effect of the present invention, the following comparative experiment was conducted: Experimental groups: model group (blank control), deer antler single-ingredient group, ginseng single-ingredient group, and the composition of the present invention group (prepared in Example 1).

[0062] Experimental methods: Mouse weighted swimming experiment, measuring swimming time, serum urea nitrogen, and liver glycogen content.

[0063] The experimental results are shown in Table 4.

[0064] Table 4

[0065] Note: Compared with the single-ingredient groups, P<0.01.

[0066] Experimental results show that, under the same amount of raw medicinal materials, the anti-fatigue effect of the composition of the present invention is significantly better than that of the simple addition of individual medicinal materials, demonstrating a good synergistic effect.

[0067] The embodiments described above are merely specific implementations of this application, used to illustrate the technical solutions of this application, and not to limit them. The protection scope of this application is not limited thereto. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that any person skilled in the art can still modify or easily conceive of changes to the technical solutions described in the foregoing embodiments within the scope of the technology disclosed in this application, or make equivalent substitutions for some of the specific technologies; and these modifications, changes, or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application. All should be covered within the protection scope of this application. Therefore, the protection scope of this application should be determined by the protection scope of the claims.

Claims

1. A pharmaceutical composition for relieving physical fatigue, characterized in that, It is composed of the following raw materials in the indicated weight ratios: 1-3 parts deer antler, 1.6-3 parts ginseng, 2-6 parts wolfberry, 2-6 parts schisandra, and 1-3 parts tortoise shell.

2. The pharmaceutical composition for relieving physical fatigue as described in claim 1, characterized in that, It is composed of the following raw materials in the indicated weight ratios: 1 part deer antler, 1.6 parts ginseng, 4 parts wolfberry, 4 parts schisandra, and 3 parts tortoise shell.

3. The pharmaceutical composition according to claim 1 or 2, characterized in that, The pharmaceutical composition is an oral solid dosage form.

4. The pharmaceutical composition according to claim 3, characterized in that, The oral solid dosage form is a tablet, granule, or capsule.

5. The method for preparing the pharmaceutical composition for relieving physical fatigue as described in claim 1 or 2, characterized in that, Includes the following steps: S1. Powder and sterilize the elk antler, and set aside. S2. Take the tortoise shell, add 6-10 times the amount of water as the medicinal material, and decoct for 1-2 hours to obtain the tortoise shell decoction; S3. Add ginseng, wolfberry and schisandra to the tortoise shell decoction in step (2), and continue to add 6-10 times the amount of water as the medicinal materials. Extract three times, each time for 1-2 hours, filter, and combine the filtrates. S4. Concentrate the filtrate from step S3 to a relative density of 1.05-1.10, and spray dry to obtain a dry powder. S5. The dry powder from step S4 is added to fine powder of deer antler, mixed well, and pharmaceutically acceptable excipients are added to prepare a pharmaceutical composition.

6. The method for preparing the pharmaceutical composition for relieving physical fatigue as described in claim 5, characterized in that, Step S5 specifically includes: mixing fine powder of deer antler and dry extract powder with white sugar powder, using 85% edible alcohol as a wetting agent to make a soft material, sieving it into particles through a 16-mesh sieve, and drying it at 50~60℃ to obtain a powder with a moisture content ≤7.0%.

7. The method for preparing the pharmaceutical composition for relieving physical fatigue as described in claim 6, characterized in that, Take the dried powder with a moisture content ≤7.0%, add magnesium stearate, mix well, compress into tablets to obtain tablets.

8. The method for preparing the pharmaceutical composition for relieving physical fatigue as described in claim 6, characterized in that, Take the dried powder with a moisture content ≤7.0%, fill it into capsules, and obtain capsules.

9. The use of the pharmaceutical composition for relieving physical fatigue as described in any one of claims 1-4, or the pharmaceutical composition for relieving physical fatigue obtained by the method described in any one of claims 5-8, in the preparation of a product for relieving physical fatigue.

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