Modified bioactive bamboo charcoal filler for sewage treatment and preparation method thereof

Abstract

The invention relates to the technical field of sewage treatment, in particular to a modified bioactive bamboo charcoal filler for sewage treatment and a preparation method thereof.The preparation method of the modified bioactive bamboo charcoal filler comprises the following steps that S1, nutrient powder is added into modified bamboo charcoal in a fluidization mode, and after fluidization is completed, the modified bamboo charcoal loaded with the nutrient powder is obtained; s2, a mixed enzyme agent is added into the modified bamboo charcoal loaded with the nutrition powder, vacuum pumping and drying are performed, and modified bioactive bamboo charcoal filler is obtained; a preparation method of the modified bamboo charcoal comprises the following steps: taking raw moso bamboos, cutting the moso bamboos into bamboo tubes with the length of 5-30cm, and performing high-temperature carbonization to obtain bamboo charcoal; spraying a hydrogen peroxide solution on the surface of the bamboo charcoal, and performing ultraviolet irradiation, washing and drying to obtain modified bamboo charcoal; or soaking the bamboo charcoal in an acid solution or an alkali solution, washing and drying to obtain modified bamboo charcoal; the method has the advantages that the sewage treatment efficiency is improved, and the service life of the filler is prolonged.

Description

Technical Field

[0001] This application relates to the technical field of wastewater treatment, and in particular to a modified biological activated bamboo charcoal packing material for wastewater treatment and its preparation method. Background Technology

[0002] In recent years, tower biofilters, rotating biological discs, biological contact oxidation, and biological fluidized beds, all belonging to the biofilm process, have received considerable research and application. The essence of the biofilm process is to fill a biochemical reaction tank with packing material, on the surface and inside of which a biofilm forms. This biofilm is an ecosystem composed of highly dense anaerobic bacteria, aerobic bacteria, facultative bacteria, fungi, protozoa, and algae. Through the metabolic activity of the microorganisms within the biofilm and the hierarchical predation of the food chain formed between the packing materials, organic pollutants in wastewater are removed, thus achieving wastewater purification. Therefore, the formation, shedding, reproduction rate, and quality of the biofilm on and inside the packing material directly affect the wastewater treatment efficiency of the biofilm process. Thus, developing packing materials with high biomass quantity and variety, biological activity, long service life, and renewability is key to the widespread application of high-biofilm processes. Summary of the Invention

[0003] In order to improve the efficiency of wastewater treatment and extend the service life of the packing material, this application provides a modified biological activated bamboo charcoal packing material for wastewater treatment and its preparation method.

[0004] In a first aspect, this application provides a method for preparing modified biological activated bamboo charcoal packing material for wastewater treatment, employing the following technical solution: A method for preparing modified biological activated bamboo charcoal packing material for wastewater treatment, comprising the following steps: S1. Nutrient powder is added to modified bamboo charcoal by fluidization. The amount of nutrient powder added is 0.5-1.5% of the mass of modified bamboo charcoal. After fluidization, modified bamboo charcoal loaded with nutrient powder is obtained. S2. Add mixed enzyme to modified bamboo charcoal loaded with nutrient powder. The mass ratio of mixed enzyme to modified bamboo charcoal is 1:(1000-3000). After spraying, vacuum press and then dry to obtain modified bioactive bamboo charcoal filler. The modified bamboo charcoal is prepared as follows: S1. Take raw bamboo, cut it into bamboo tubes 5-30cm long, and carbonize it at high temperature to obtain bamboo charcoal; S2. Spray hydrogen peroxide solution onto the surface of bamboo charcoal, then irradiate it under a UV lamp with a wavelength of 180-330nm for 15-24 hours, wash and dry, repeat 2-3 times to obtain modified bamboo charcoal. Alternatively, bamboo charcoal can be soaked in an acidic or alkaline solution for 48-120 hours, washed, and dried to obtain modified bamboo charcoal.

[0005] By adopting the above technical solution, this application uses bamboo charcoal as the filler matrix. The naturally hollow and porous structure of bamboo charcoal provides a large specific surface area, porosity, and mechanical strength, offering ample space for microbial attachment while resisting water erosion, ensuring service life and water treatment efficiency. Furthermore, this application modifies the bamboo charcoal through hydrogen peroxide / ultraviolet light treatment, or treatment with acid or alkali solutions, effectively removing the microcrystalline structures formed after sintering, thus expanding the pores and better utilizing the porous structure of bamboo charcoal to improve biofilm formation rate. Additionally, loading nutrient powder onto the surface of the bamboo charcoal provides the initial nutrients needed for microbial growth, accelerating and improving biofilm formation efficiency. Therefore, by modifying and expanding the pores of bamboo charcoal, increasing its specific surface area and biofilm formation rate, the treatment efficiency for wastewater can be improved.

[0006] Preferably, the nutritional powder comprises the following raw materials in the following weight percentages: 10-20% brown sugar, 10-20% peptone powder, 5-15% wheat starch, 0.1-0.5% sodium chloride, 0.1-0.5% K2HPO4, 0.01-0.3% Ca(NO3)2, 0.03-0.07% biotin, 3-5% yeast, 7-11% diatomaceous earth, and the balance being corn starch.

[0007] By employing the above technical solutions, this formula constructs a complete microbial growth culture medium. Brown sugar and starch provide energy and are the main raw materials for synthesizing the cytoskeleton. Peptone provides organic nitrogen and amino acids; yeast is not only an excellent source of nitrogen and vitamins, but it is also a living microbial seed, providing the initial inoculum for biofilm formation. Diatomaceous earth itself has a porous structure, which can adsorb and slowly release nutrients; corn starch serves as a filler and a slow-release carbon source. The combined effect of these two elements allows the nutrient powder to be released slowly and continuously within the bamboo charcoal, rather than being dissolved all at once, which is the core guarantee for the long-term effectiveness of the filler. K₂HPO₄ provides phosphorus and potassium; Ca(NO₃)₂ provides calcium and nitrogen; NaCl provides sodium and chloride ions. Furthermore, the inventors of this application unexpectedly discovered that the addition of sodium chloride can accelerate the biofilm formation rate, shortening it by about half, thus greatly improving wastewater treatment efficiency. Analysis shows that sodium chloride can provide potential and electrolytes for various bacterial species, accelerate nutrient uptake, provide suitable ionic strength, help stabilize the microbial cell membrane structure, promote transmembrane transport of nutrients, thereby enhancing the structural stability and metabolic activity of the biofilm and improving biofilm formation efficiency.

[0008] Preferably, the pH of the acid solution is 5.2-5.8.

[0009] Preferably, the pH value of the alkaline solution is 7.2-7.8.

[0010] By adopting the above technical solutions, both weak acid and weak alkaline conditions can effectively destroy the crystals formed during the sintering of bamboo charcoal, thereby expanding the pores and improving the enrichment and adhesion of various microorganisms.

[0011] Preferably, the nutritional powder also includes a penetrant at a mass percentage of 0.1-0.5%.

[0012] By adopting the above technical solution, the penetrant can improve the wettability between the fungal agent and bamboo charcoal, which is conducive to complete biofilm formation.

[0013] Preferably, the penetrant is a mixture of polysorbate 80 and alkyl glycoside APG-0810.

[0014] By employing the above technical solutions, both polysorbate 80 (Tween 80) and APG-0810 are highly efficient nonionic surfactants that can significantly reduce the surface tension of the mixed enzyme aqueous solution, making it easier to wet the bamboo charcoal surface. This is especially true for modified bamboo charcoal with complex surface chemistry; the compounded penetrant solution exhibits superior wettability and spreadability, enabling capillary action to propel the subsequently sprayed mixed enzyme to penetrate deeper and more uniformly into the micropores and mesopores of the bamboo charcoal, rather than merely remaining on the surface. This ensures the depth and uniformity of enzyme loading, resulting in higher and more sustained overall catalytic activity of the filler. Furthermore, a synergistic effect was observed; the two surfactant molecules co-adsorb and align at the solution surface and the liquid / solid (bamboo charcoal) interface. APG, due to its rapid adsorption characteristics, first reduces interfacial tension, creating conditions for the adsorption and alignment of Tween 80. The two molecules form a tighter and more stable mixed adsorption film through intermolecular forces. This makes the critical micelle concentration (CMC) of the mixed solution lower than the CMC of either molecule when used alone. This means that the best surface tension reduction effect can be achieved with a lower concentration, resulting in a significant synergistic effect.

[0015] Preferably, the mixed enzyme preparation comprises the following raw materials in the following mass percentages: protease 3-7%, lipase 4-8%, amylase 17-23%, phospholipase 2-4%, α-amylase 13-17%, carboxymethyl cellulose 0.5-1.5%, with the balance being water.

[0016] By employing the above-mentioned technical solutions, proteases hydrolyze protein-based pollutants; lipases decompose oily pollutants; and amylases and α-amylases synergistically degrade starch and polysaccharide pollutants. This almost completely covers the main organic components in domestic sewage and various food and livestock wastewater. Phospholipases help decompose microbial cell membrane fragments (from dead cells), promoting further recycling of organic matter; catalase (though not listed in this claim, it is included in the original document) can decompose substances such as hydrogen peroxide that are toxic to microorganisms, protecting the biofilm. These enzymes form a "biocatalytic layer" on the surface and inside the packing material, which can pre-decompose complex macromolecular organic matter in sewage into smaller molecular organic matter (such as amino acids, fatty acids, and monosaccharides). This produces dual benefits: firstly, it significantly reduces the difficulty and energy consumption of subsequent microbial metabolism, accelerating the growth, reproduction, and biofilm formation of microorganisms; secondly, it improves the overall degradation efficiency and rate of pollutants, especially during system startup or when subjected to high load shocks, where this "enzymatic pretreatment" effect is crucial.

[0017] Secondly, this application provides a modified biological activated bamboo charcoal packing material for wastewater treatment, employing the following technical solution: A modified biologically activated bamboo charcoal packing material for wastewater treatment is prepared by the aforementioned method for preparing a modified biologically activated bamboo charcoal packing material for wastewater treatment.

[0018] In summary, this application includes at least one of the following beneficial technical effects: 1. This application uses bamboo charcoal as the filler matrix. The naturally hollow and porous structure of bamboo charcoal provides a large specific surface area, porosity, and mechanical strength, offering ample space for microbial attachment while resisting water erosion, ensuring service life and water treatment efficiency. Furthermore, this application modifies the bamboo charcoal through hydrogen peroxide / ultraviolet light treatment, or acid or alkaline treatment, effectively removing the microcrystalline structures formed after sintering, thus expanding the pores and better utilizing the porous structure of bamboo charcoal to improve biofilm formation rate. Additionally, loading nutrient powder onto the bamboo charcoal surface provides the initial nutrients needed for microbial growth, accelerating and improving biofilm formation efficiency. Therefore, by modifying and expanding the pores of bamboo charcoal, increasing its specific surface area and biofilm formation rate, the treatment efficiency for wastewater can be improved.

[0019] 2. Both polysorbate 80 (Tween 80) and APG-0810 are highly efficient nonionic surfactants that significantly reduce the surface tension of the mixed enzyme aqueous solution, making it easier to wet the bamboo charcoal surface, especially for modified bamboo charcoal with complex surface chemistry. The compounded penetrant solution exhibits superior wettability and spreadability, enabling capillary action to propel the subsequently sprayed mixed enzyme to penetrate deeper and more uniformly into the micropores and mesopores of the bamboo charcoal, rather than merely remaining on the surface. This ensures the depth and uniformity of enzyme loading, resulting in higher and more sustained overall catalytic activity of the filler. Furthermore, a synergistic effect was observed, with both surfactant molecules co-adsorbing and aligning at the solution surface and the liquid / solid (bamboo charcoal) interface. APG, due to its rapid adsorption characteristics, first reduces interfacial tension, creating conditions for the adsorption and alignment of Tween 80. The two molecules form a tighter and more stable mixed adsorption film through intermolecular forces. This makes the critical micelle concentration (CMC) of the mixed solution lower than the CMC of either molecule when used alone. This means that the best surface tension reduction effect can be achieved with a lower concentration, resulting in a significant synergistic effect. Detailed Implementation

[0020] The following provides a more detailed description of this application in conjunction with specific details.

[0021] raw material All raw materials used in the preparation examples and embodiments of this application are commercially available products. Among them, brown sugar, peptone powder, wheat starch, corn starch, carboxymethyl cellulose, and biotin are all food grade; the particle size of diatomaceous earth is 400 mesh; the protease is a neutral protease (50,000 u / g), purchased from Henan Anrui Biotechnology Co., Ltd.; the lipase is industrial grade 100,000 u / g, the amylase is food grade glucosylamylase 260,000 u / mL, the α-amylase is food grade 120,000 u / mL, and the phospholipase is food grade 3,000 u / g, all purchased from Ningxia Xiasheng Industrial Group Co., Ltd.

[0022] Preparation Example Preparation Example 1 A modified bamboo charcoal, the preparation method of which is as follows: S1. Take 2.5-5 year old moso bamboo, cut it into 5cm long bamboo tubes, and carbonize it completely at 780℃ to obtain bamboo charcoal. S2. Spray a 12.5% ​​hydrogen peroxide solution onto the surface of bamboo charcoal to wet it. The amount of hydrogen peroxide solution used is about 70% of the mass of bamboo charcoal. Then, irradiate it under a 220nm ultraviolet lamp for 20 hours. After washing with water, dry it. Repeat the whole process 2-3 times to obtain modified bamboo charcoal.

[0023] Preparation Example 2 A modified bamboo charcoal, the preparation method of which is as follows: S1. Take 2.5-5 year old moso bamboo, cut it into 30cm long bamboo tubes, and carbonize it completely at 1100℃ to obtain bamboo charcoal. S2. The bamboo charcoal is completely immersed in hydrochloric acid solution with pH controlled at 5.2-5.8 for 80 hours. After washing with clean water, it is dried to obtain modified bamboo charcoal.

[0024] Preparation Example 3 A modified bamboo charcoal, the preparation method of which is as follows: S1. Take 2.5-5 year old moso bamboo, cut it into 20cm long bamboo tubes, and carbonize it completely at 1100℃ to obtain bamboo charcoal. S2. The bamboo charcoal is completely soaked in a sodium hydroxide solution with the pH controlled at 7.2-7.8 for 60 hours. After washing with clean water, it is dried to obtain modified bamboo charcoal. Example

[0025] Example 1 A modified biological activated bamboo charcoal packing material for wastewater treatment is prepared as follows: S1. Take the modified bamboo charcoal of Preparation Example 1 into a fluidization tower, add nutrient powder in a fluidization manner, the amount of nutrient powder added is 1% of the mass of the modified bamboo charcoal, the fluidization time in the fluidization tower is 30 minutes, after the fluidization is completed, take out the modified bamboo charcoal loaded with nutrient powder, and mark the fluidization direction. S2. Add mixed enzymes to modified bamboo charcoal loaded with nutrient powder by spraying. The mass ratio of mixed enzymes to modified bamboo charcoal is 1:1500. After spraying, vacuum (50 kPa negative pressure) is applied for 70 minutes, and then dried at 37°C for 36 hours to obtain modified bioactive bamboo charcoal filler.

[0026] The nutrient powder, by weight percentage, comprises the following ingredients: 15% brown sugar, 15% peptone dry powder, 10% wheat starch, 0.5% sodium chloride, 0.3% K2HPO4, 0.1% Ca(NO3)2, 0.05% biotin, 4% yeast (purchased from Angel Yeast Co., Ltd., packaged as "High-Activity Dry Yeast," with a net content of 500g / bag, low-sugar type), 9% diatomaceous earth, and the remainder being corn starch. The nutrient powder provides a nutritional basis for the growth of microorganisms generated in the initial stage of wastewater treatment. The mixed enzyme preparation comprises the following raw materials by mass percentage: 5% protease, 6% lipase, 20% amylase, 2% phospholipase, 15% α-amylase, 1% carboxymethyl cellulose, and the remainder is water; the raw materials of the mixed enzyme preparation are stirred at 500 r / h at room temperature for 30 minutes to obtain the preparation.

[0027] Example 2 A modified biological activated bamboo charcoal packing material for wastewater treatment is prepared as follows: S1. Take the modified bamboo charcoal of Preparation Example 2 into a fluidization tower and add nutrient powder in a fluidized manner. The amount of nutrient powder added is 1% of the mass of the modified bamboo charcoal. The fluidization time in the fluidization tower is 40 minutes. After the fluidization is completed, take out the modified bamboo charcoal loaded with nutrient powder and mark the fluidization direction. S2. Add mixed enzymes to modified bamboo charcoal loaded with nutrient powder by spraying. The mass ratio of mixed enzymes to modified bamboo charcoal is 1:1500. After spraying, vacuum (50 kPa negative pressure) is applied for 50 minutes, and then dried at 37°C for 24 hours to obtain modified bioactive bamboo charcoal filler.

[0028] The nutrient powder, by weight percentage, comprises the following ingredients: 10% brown sugar, 20% peptone dry powder, 15% wheat starch, 0.5% sodium chloride, 0.1% K2HPO4, 0.3% Ca(NO3)2, 0.03% biotin, 5% yeast (purchased from Angel Yeast Co., Ltd., packaged as "High-Activity Dry Yeast," with a net content of 500g / bag, low-sugar type), 7% diatomaceous earth, and the remainder being corn starch. The nutrient powder provides a nutritional basis for the growth of microorganisms generated in the initial stage of wastewater treatment. The mixed enzyme preparation comprises the following raw materials by mass percentage: 3% protease, 8% lipase, 17% amylase, 4% phospholipase, 13% α-amylase, 0.5% carboxymethyl cellulose, and the remainder is water; the raw materials of the mixed enzyme preparation are stirred at 500 r / h at room temperature for 30 minutes to obtain the preparation.

[0029] Example 3 A modified biological activated bamboo charcoal packing material for wastewater treatment is prepared as follows: S1. Take the modified bamboo charcoal of Preparation Example 3 into a fluidization tower and add nutrient powder in a fluidization manner. The amount of nutrient powder added is 1% of the mass of the modified bamboo charcoal. The fluidization time in the fluidization tower is 40 minutes. After the fluidization is completed, take out the modified bamboo charcoal loaded with nutrient powder and mark the fluidization direction. S2. Add mixed enzymes to modified bamboo charcoal loaded with nutrient powder by spraying. The mass ratio of mixed enzymes to modified bamboo charcoal is 1:1000. After spraying, vacuum (50 kPa negative pressure) is applied for 50 minutes, and then dried at 37°C for 24 hours to obtain modified bioactive bamboo charcoal filler.

[0030] The nutrient powder, by weight percentage, comprises the following ingredients: 20% brown sugar, 10% peptone dry powder, 5% wheat starch, 0.5% sodium chloride, 0.5% K2HPO4, 0.01% Ca(NO3)2, 0.07% biotin, 3% yeast (purchased from Angel Yeast Co., Ltd., packaged as "High-Activity Dry Yeast," with a net content of 500g / bag, low-sugar type), 11% diatomaceous earth, and the remainder being corn starch. The nutrient powder provides a nutritional basis for the growth of microorganisms generated in the initial stage of wastewater treatment. The mixed enzyme preparation comprises the following raw materials by mass percentage: 3% protease, 8% lipase, 17% amylase, 4% phospholipase, 13% α-amylase, 0.5% carboxymethyl cellulose, and the remainder is water; the raw materials of the mixed enzyme preparation are stirred at 500 r / h at room temperature for 30 minutes to obtain the preparation.

[0031] Example 4 A modified biological activated bamboo charcoal packing material for wastewater treatment differs from Example 1 in that the mass percentage of sodium chloride added is 0.1%, while the remaining steps are the same as in Example 1.

[0032] Example 5 A modified biological activated bamboo charcoal packing material for wastewater treatment differs from Example 1 in that the mass percentage of sodium chloride added is 0.3%, while the remaining steps are the same as in Example 1.

[0033] Example 6 A modified biologically activated bamboo charcoal filler for wastewater treatment differs from Example 5 in that its nutrient powder further includes 0.1% by mass of a penetrant, which is a mixture of polysorbate 80 and alkyl glycoside APG-0810 in a mass ratio of 3:1. The remaining steps are the same as in Example 5.

[0034] Example 7 A modified biological activated bamboo charcoal packing material for wastewater treatment differs from Example 6 in that the mass percentage of the permeabilizer added is 0.5%, while the remaining steps are the same as in Example 6.

[0035] Example 8 A modified biologically activated bamboo charcoal packing material for wastewater treatment differs from Example 6 in that its penetrant is polysorbate 80, while the remaining steps are the same as in Example 6.

[0036] Example 9 A modified biological activated bamboo charcoal packing material for wastewater treatment differs from Example 6 in that its penetrant is alkyl glycoside APG-0810, while the remaining steps are the same as in Example 6.

[0037] Comparative Example Comparative Example 1 A modified biological activated bamboo charcoal filler for wastewater treatment differs from Example 1 in that its nutrient powder does not contain added sodium chloride, while the remaining steps are the same as in Example 1.

[0038] Comparative Example 2 A modified biological activated bamboo charcoal packing material for sewage treatment differs from Example 1 in that the modified bamboo charcoal is replaced with unmodified bamboo charcoal, while the remaining steps are the same as in Example 1.

[0039] Performance testing Detection methods / test methods Modified bioactive bamboo charcoal fillers were prepared according to the preparation methods of Examples 1-8 and Comparative Examples 1-2, and then tested according to the following detection methods. The test results are shown in Table 1.

[0040] Biofilm attachment operation steps: Each sample of modified biological activated bamboo charcoal packing was placed in a contact oxidation tank, with the amount distributed at 20% of the tank capacity. Wastewater to be treated was injected into the tank. The wastewater was domestic sewage from the Zhangzhou Qibin Glass Co., Ltd. The raw water influent indicators were: COD = 160 mg / L, NH3-N = 36 mg / L, and dissolved oxygen concentration was maintained at 2-4 mg / L. The daily influent volume was controlled at 150 tons. The number of days required for biofilm formation was observed.

[0041] Wastewater treatment: Modified bio-activated bamboo charcoal packing material with biofilm attached was used for treating domestic wastewater from the Zhangzhou Qibin Glass Co., Ltd. The influent raw water parameters were: COD = 160 mg / L, NH3-N = 36 mg / L. The treatment process was a biological contact oxidation process: contact anaerobic tank + contact facultative anaerobic tank + primary sedimentation tank + contact oxidation tank + secondary sedimentation tank. The modified bio-activated bamboo charcoal packing material with biofilm attached was distributed at 20% of the tank capacity in the contact oxidation tank, with a daily treatment capacity of 150 tons. The effluent water quality parameters were: COD = 12.40 mg / L, NH3-N = 0.46 mg / L, meeting the urban greening reuse water standard, with removal rates of 92.25% and 98.72%, respectively. The wastewater treatment performance of each modified bio-activated bamboo charcoal packing material was tested.

[0042] Table 1 Test Results

[0043] As can be seen from the test data in Table 1, the biofilm of this application has excellent efficiency. The modified biological activated bamboo charcoal packing material prepared has excellent treatment effect on sewage after biofilm formation. With a daily treatment capacity of 150 tons, its effluent can meet the standards for urban greening reuse water.

[0044] In combination with Example 1 and Comparative Example 1, the addition of sodium chloride can accelerate the biofilm formation rate, shortening it by about half, and greatly improving the wastewater treatment efficiency.

[0045] In conjunction with Example 1 and Comparative Example 2, this application modifies bamboo charcoal by treating it with hydrogen peroxide / ultraviolet light, acid, or alkali. These methods effectively remove the micro-crystals formed after bamboo charcoal sintering, thereby expanding the pores, better utilizing the porous structure of bamboo charcoal, increasing the biofilm formation rate, and improving the wastewater treatment effect.

[0046] In conjunction with Examples 5-9, the penetrant improves the wettability between the microbial agent and bamboo charcoal, facilitating biofilm formation within the pores of the bamboo charcoal. Both polysorbate 80 (Tween 80) and APG-0810 are highly efficient nonionic surfactants that significantly reduce the surface tension of the mixed enzyme aqueous solution, making it easier to wet the bamboo charcoal surface, especially for modified bamboo charcoal with complex surface chemistry. The compounded penetrant solution exhibits superior wettability and spreadability, enabling capillary action to propel the subsequently sprayed mixed enzyme to penetrate deeper and more uniformly into the micropores and mesopores of the bamboo charcoal, rather than merely remaining on the surface. This ensures the depth and uniformity of enzyme loading, resulting in higher and more sustained overall catalytic activity of the filler. Furthermore, a synergistic effect was observed; the two surfactant molecules co-adsorb and align at the solution surface and the liquid / solid (bamboo charcoal) interface. APG, due to its rapid adsorption characteristics, first reduces interfacial tension, creating conditions for the adsorption and alignment of Tween 80. The two molecules form a tighter and more stable mixed adsorption film through intermolecular forces. This makes the critical micelle concentration (CMC) of the mixed solution lower than the CMC of either molecule when used alone. This means that the best surface tension reduction effect can be achieved with a lower concentration, resulting in a significant synergistic effect.

[0047] This specific embodiment is merely an explanation of the present invention and is not intended to limit the invention. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A method for preparing modified biological activated bamboo charcoal packing material for wastewater treatment, characterized in that: It includes the following steps: S1. Nutrient powder is added to modified bamboo charcoal by fluidization. The amount of nutrient powder added is 0.5-1.5% of the mass of modified bamboo charcoal. After fluidization, modified bamboo charcoal loaded with nutrient powder is obtained. S2. Add mixed enzyme to modified bamboo charcoal loaded with nutrient powder. The mass ratio of mixed enzyme to modified bamboo charcoal is 1:(1000-3000). After spraying, vacuum press and then dry to obtain modified bioactive bamboo charcoal filler. The modified bamboo charcoal is prepared as follows: S1. Take raw bamboo, cut it into bamboo tubes 5-30cm long, and carbonize it at high temperature to obtain bamboo charcoal; S2. Spray hydrogen peroxide solution onto the surface of bamboo charcoal, then irradiate it under a UV lamp with a wavelength of 180-330nm for 15-24 hours, wash and dry, repeat 2-3 times to obtain modified bamboo charcoal. Alternatively, bamboo charcoal can be soaked in an acidic or alkaline solution for 48-120 hours, washed, and dried to obtain modified bamboo charcoal.

2. The method for preparing modified biological activated bamboo charcoal packing material for wastewater treatment according to claim 1, characterized in that: The nutritional powder comprises the following raw materials in the following weight percentages: 10-20% brown sugar, 10-20% peptone powder, 5-15% wheat starch, 0.1-0.5% sodium chloride, 0.1-0.5% K2HPO4, 0.01-0.3% Ca(NO3)2, 0.03-0.07% biotin, 3-5% yeast, 7-11% diatomaceous earth, and the balance being corn starch.

3. The method for preparing a modified biological activated bamboo charcoal packing material for wastewater treatment according to claim 1, characterized in that: The pH of the acid solution is 5.2-5.

8.

4. The method for preparing a modified biological activated bamboo charcoal packing material for wastewater treatment according to claim 1, characterized in that: The pH value of the alkaline solution is 7.2-7.

8.

5. The method for preparing modified biological activated bamboo charcoal packing material for sewage treatment according to claim 2, characterized in that: The nutritional powder also includes a penetrant at a mass percentage of 0.1-0.5%.

6. The method for preparing a modified biological activated bamboo charcoal packing material for wastewater treatment according to claim 5, characterized in that: The penetrant is a mixture of polysorbate 80 and alkyl glycoside APG-0810.

7. The method for preparing a modified biological activated bamboo charcoal packing material for wastewater treatment according to claim 1, characterized in that: The mixed enzyme preparation comprises the following raw materials in the following mass percentages: protease 3-7%, lipase 4-8%, amylase 17-23%, phospholipase 2-4%, α-amylase 13-17%, carboxymethyl cellulose 0.5-1.5%, with the balance being water.

8. A modified biological activated bamboo charcoal packing material for wastewater treatment, characterized in that: It is prepared by the method described in any one of claims 1-7 for the preparation of a modified biological activated bamboo charcoal packing material for sewage treatment.

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