Double-module oscillation type resuscitation device for high-capacity frozen biological agent

Abstract

The invention belongs to the technical field of biological sample preservation and resuscitation, and provides a double-module oscillation type resuscitation device for a high-capacity frozen biological agent, the double-module oscillation type resuscitation device comprises a fixed base, the top of the fixed base is movably connected with an oscillation shell box, and one side of the fixed base is fixedly connected with a console; an upper heating module is arranged in the oscillation shell box, and the bottom of the upper heating module is connected with a lower heating module through a hinge; the upper heating module and the lower heating module are semi-cylindrical and are made of Peltier constant-temperature metal blocks, and temperature sensors are fixedly mounted on the inner walls of the upper heating module and the lower heating module; the inner wall of the bottom of the fixed base is fixedly connected with a fixed plate. The upper heating module and the lower heating module are both semi-cylindrical Peltier constant-temperature metal blocks, 360-degree omnibearing tight wrapping can be formed on a columnar sample (such as a 50mL injector) after the upper heating module and the lower heating module are closed, and a great contact area and a high-efficiency heat conduction path which are far better than those of traditional water bath or plane heating are provided.

Description

Technical Field

[0001] This invention belongs to the field of biological sample preservation and resuscitation technology, and specifically relates to a dual-module oscillating resuscitation device for large-volume frozen biological agents. Background Technology

[0002] In the medical field, intestinal microbiota transplantation (IVPP) technology is becoming increasingly widespread, bringing new hope and pathways to the treatment of numerous diseases. As a key carrier in this technology, the preservation and resuscitation quality of the intestinal microbiota directly affects the effectiveness and safety of the treatment. Typically, the intestinal microbiota is preserved in 50mL syringes or centrifuge tubes and frozen at -80°C to maximize the activity and stability of the microbiota.

[0003] The resuscitation process is crucial in the use of intestinal flora solutions. To protect the activity of intestinal flora, following a "rapid dissolution" strategy is key, requiring the sample to be completely and uniformly dissolved within 1 to 2 minutes. If the resuscitation time exceeds this range, the survival rate of intestinal flora will decrease significantly, thereby affecting the activity and structure of the flora, ultimately adversely impacting the effectiveness and safety of the treatment.

[0004] Currently, water baths are commonly used in laboratories for the resuscitation of intestinal bacterial solutions. However, this method has several significant drawbacks. First, the thawing speed is extremely slow, resulting in low resuscitation efficiency. It typically takes 15-30 minutes to thaw completely from -80°C. This lengthy thawing process causes a large number of intestinal bacteria to die due to ice crystal formation from prolonged exposure to low temperatures, directly leading to reduced intestinal bacterial activity, altered bacterial community structure, and severely impacting subsequent treatment outcomes. Second, temperature control is not precise enough. During operation, the water temperature in the water bath is prone to significant fluctuations. Since intestinal bacteria are extremely sensitive to temperature, instability can easily lead to the inactivation of some temperature-sensitive bacteria, further weakening the overall quality of the intestinal bacterial solution. Third, uneven heating is a prominent issue. For large-volume samples (e.g., 50 mL), there is a significant temperature difference between the sample core and the outside, making it difficult to thaw synchronously. This uneven heating significantly reduces the activity of intestinal bacteria inside the frozen sample, greatly diminishing the resuscitation effect.

[0005] To overcome the contamination problems associated with water bath resuscitation, dry-type incubators have been developed. While dry-type incubators avoid the potential contamination caused by water baths to some extent, they still suffer from uneven heating when processing large volumes of samples. Because they use static heating, they cannot achieve uniform heating of the samples, lack a shaking mixing function, and cannot provide 360° all-around heating of the samples. This results in uneven heating, low resuscitation efficiency, and a significant impact on bacterial activity, making it difficult to meet the high resuscitation quality requirements of intestinal microbiota transplantation technology.

[0006] Therefore, developing a device capable of rapidly, uniformly, and effectively thawing large volumes of frozen biological agents is of great significance for improving the therapeutic efficacy and safety of intestinal microbiota transplantation (IVPP) technology. This invention aims to provide a dual-module oscillating thawing device for large volumes of frozen biological agents, thereby addressing the problems of biological sample preservation and thawing mentioned in the background art. Summary of the Invention

[0007] To address the above-mentioned technical problems, this invention proposes a dual-module oscillating resuscitation device for large-volume frozen biological preparations, thereby resolving the issues of biological sample preservation and resuscitation mentioned in the background art.

[0008] The technical solution of this invention is: This invention proposes a dual-module oscillating resuscitation device for large-volume frozen biological agents, including a fixed base, an oscillating outer shell movably connected to the top of the fixed base, and a control console fixedly connected to one side of the fixed base. The oscillating housing contains an upper heating module, and the bottom of the upper heating module is connected to a lower heating module via a hinge. Both the upper heating module and the lower heating module are semi-cylindrical and made of Peltier thermostatic metal blocks. Temperature sensors are fixedly installed on the inner walls of both modules. A fixing plate is fixedly connected to the bottom inner wall of the fixed base, and a first servo motor is fixedly connected to one side of the fixing plate. The movable end of the first servo motor passes through the fixing plate and is fixedly connected to a drive turntable. A centrifugal rod is fixedly connected to one side of the drive turntable, a swing rod is movably connected to one end of the centrifugal rod, a half gear is fixedly connected to one end of the swing rod, and a moving rod is meshed with one side of the half gear. Both ends of the moving rod are fixedly connected to connecting plates, and the tops of the two connecting plates are fixedly connected to the bottom of the oscillating housing. The oscillating housing is equipped with a shaking device for making the heating module swing in a fan shape.

[0009] Preferably, the shaking device includes a second servo motor, a linkage rod, a sliding rail, a rack, a drive gear, a rotating rod, and a placement block; The movable end of the second servo motor is fixedly connected to a linkage rod through the oscillating housing, and one end of the linkage rod is movably connected to a sliding rail. A rack is fixedly connected to one side of the sliding track via a fixing block. A drive gear is meshed with the top of the rack, and a rotating rod is fixedly connected to the center of the drive gear. One end of the rotating rod passes through the partition plate and is fixedly connected to a placement block.

[0010] Preferably, temperature sensors are fixedly installed on the inner walls of both the upper heating module and the lower heating module to precisely control the heating temperature.

[0011] Preferably, a slider is fixedly connected to the top of the fixing plate, and a limit rod is fixedly connected to one side of the fixing plate; The bottom of the oscillating housing is provided with an elongated groove, and the top of the slider is slidably connected to the inside of the elongated groove; Two limiting plates are fixedly connected to one side of the fixed plate above the limiting rod.

[0012] Preferably, a sliding groove is provided on one side of the swing rod, and one end of the centrifugal rod is movably connected inside the sliding groove; One end of the limit rod is rotatably connected to one side of the swing rod.

[0013] Preferably, the movable rod is movably connected between the two limiting plates, and both ends of the movable rod pass through the two limiting plates respectively for extension; Two oscillating springs are fixedly connected between the two ends of the two moving rods and the two limiting plates.

[0014] Preferably, the interior of the oscillating housing is divided into a placement space and a driving space by a partition plate; The shaking device is located inside the drive space, and the upper heating module and the lower heating module are located inside the placement space.

[0015] Preferably, one end of the linkage rod is movably connected to a linkage slider, and one end of the linkage slider is slidably connected to the inner wall of the sliding track.

[0016] Preferably, the rack and sliding track are arranged in a cross shape, and a connecting groove is provided on one side of the partition plate; One side of the rack is slidably connected inside the connecting groove.

[0017] Preferably, the placement block is semi-cylindrical, and a threaded push rod is threadedly connected to one side of the placement block; One end of the threaded push rod passes through the placement block and is rotatably connected to the push block.

[0018] The present invention has the following advantages and effects compared with the prior art: (1) Both the upper and lower heating modules are semi-cylindrical Peltier thermostatic metal blocks. When closed, they can form a 360° all-round tight wrap around the cylindrical sample (such as a 50mL syringe), providing a much larger contact area and a more efficient heat conduction path than traditional water baths or planar heating. At the same time, the horizontal swing mechanism (drive turntable, swing rod, half gear and moving rod) driven by the first servo motor makes the entire oscillating outer shell swing back and forth; the shaking device (linkage rod, rack and drive gear) driven by the second servo motor makes the sample carrier block (placement block) swing independently in a fan shape up and down. The combination of these two orthogonal oscillating motions creates intense and uniform eddies and shear forces within the sample, instantly breaking down melting ice crystals and rapidly homogenizing the temperature and composition of all parts of the sample. This design fundamentally solves the core problem of large temperature differences and uneven heating between the core and the outside when thawing large-volume samples, enabling the sample to reach a completely and uniformly thawed state in a very short time of 1-2 minutes, thus maximizing the protection of the activity and integrity of the bacterial community structure of frozen biological agents (such as enteric bacterial solutions).

[0019] (2) The integrated temperature sensor in the heating module, combined with Peltier technology, enables millisecond-level monitoring and high-precision closed-loop control of the heating surface temperature, ensuring that the heating temperature remains strictly stable at the set value (e.g., 37°C), thus avoiding the damage to heat-sensitive biological samples caused by temperature fluctuations in traditional water baths. The control console has a preset intelligent resuscitation program: after the rapid heating and thawing phase, it can automatically instruct the system to quickly lower the temperature to and maintain it at a constant temperature of 37°C, suitable for biological activity, and continue to perform heat preservation and oscillation until the set time. The entire process is completed automatically without manual intervention or timing, eliminating human error, ensuring high consistency of resuscitation conditions and reproducibility of results for each resuscitation, and significantly improving the standardization level of the experiment and the safety of the treatment.

[0020] (3) The horizontal oscillation mechanism uses an eccentric turntable and a swing rod to convert rotational motion into reciprocating oscillation, and achieves force transmission and reversal through the meshing of a half gear and a moving rod. The structure is compact and the operation is stable. The shaking device adopts a combination of connecting rod and gear rack to convert rotational motion into linear motion of the rack and then drive the gear to rotate reciprocally, thereby realizing the fan-shaped oscillation of the sample. The motion conversion is efficient and reliable. The addition of the oscillation spring plays a role in buffering and assisting, making the oscillation process gentler and more energy-efficient. In addition, the heating module is connected by a hinge, which is convenient to open and close and facilitates the handling of samples. The threaded push rod and push block design on the side of the placement block can easily secure the heating module and prevent slippage during oscillation. The overall device integrates the drive system, oscillation system and heating system into a fixed base and oscillation housing. The layout is reasonable and the appearance is neat. The operation can be completed with one button on the control panel, which greatly improves the user experience and laboratory work efficiency. Attached Figure Description

[0021] Figure 1 This is an overall diagram of the present invention; Figure 2 This is a side view of the present invention; Figure 3 This is an internal view of the fixing base of the present invention; Figure 4 This is a schematic diagram of the structure on the fixing plate of the present invention; Figure 5 This is an internal view of the oscillating housing of the present invention; Figure 6 This is a schematic diagram of the placement block structure of the present invention; Figure 7 This is a schematic diagram of the shaking device of the present invention.

[0022] Figure label: 1. Fixed base; 2. Vibration housing; 3. Control console; 4. Upper heating module; 5. Heating lower module; 6. Fixing plate; 7. First servo motor; 8. Drive turntable; 9. Centrifugal rod; 10. Swing rod; 11. Limiting rod; 12. Half gear; 13. Limiting plate; 14. Moving rod; 15. Oscillating spring; 16. Connecting plate; 17. Slider; 18. Second servo motor; 19. Linkage rod; 20. Sliding rail; 21. Rack; 22. Drive gear; 23. Rotating rod; 24. Placement block; 25. Threaded push rod; 26. Push block. Detailed Implementation

[0023] To enable those skilled in the art to better understand the present invention, the invention will now be further described in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are for illustrative and explanatory purposes only and are not intended to limit the invention.

[0024] Example 1: like Figures 1-7 As shown, the present invention provides a dual-module oscillating resuscitation device for large-volume frozen biological agents, including a fixed base 1, an oscillating outer shell 2 movably connected to the top of the fixed base, and a control console 3 fixedly connected to one side of the fixed base; The oscillating housing 2 is equipped with an upper heating module 4, and the bottom of the upper heating module 4 is connected to a lower heating module 5 via a hinge. Both the upper heating module 4 and the lower heating module 5 are semi-cylindrical and made of Peltier thermostatic metal blocks. Temperature sensors are fixedly installed on the inner walls of both modules. A fixing plate 6 is fixedly connected to the bottom inner wall of the fixed base 1. A first servo motor 7 is fixedly connected to one side of the fixing plate 6. The movable end of the first servo motor 7 passes through the fixing plate 6 and is fixedly connected to a drive turntable 8. A centrifugal rod 9 is fixedly connected to one side of the drive turntable 8. A swing rod 10 is movably connected to one end of the centrifugal rod 9. A half gear 12 is fixedly connected to one end of the swing rod 10. A moving rod 14 is meshed with one side of the half gear 12. Both ends of the moving rod 14 are fixedly connected to connecting plates 16, and the top ends of the two connecting plates 16 are fixedly connected to the bottom of the oscillating housing 2. The oscillating housing 2 is equipped with a shaking device for making the heating lower module 5 swing in a fan shape.

[0025] The shaking device includes a second servo motor 18, a linkage rod 19, a sliding rail 20, a rack 21, a drive gear 22, a rotating rod 23, and a placement block 24; The movable end of the second servo motor 18 passes through the oscillating housing 2 and is fixedly connected to a linkage rod 19. One end of the linkage rod 19 is movably connected to a sliding rail 20. A rack 21 is fixedly connected to one side of the sliding track 20 via a fixing block. A drive gear 22 is meshed with the top of the rack 21. A rotating rod 23 is fixedly connected to the center of the drive gear 22. One end of the rotating rod 23 passes through the partition plate and is fixedly connected to the placement block 24.

[0026] The second servo motor 18 is started by the control console 3, causing the movable end of the second servo motor 18 to drive the linkage rod 19 to rotate (one end of the linkage rod 19 moves in a circle around the movable end of the second servo motor 18). This causes one end of the linkage rod 19 to drive the rack 21 to move left and right on the partition plate through the sliding rail 20. Then, through the meshing connection between the rack 21 and the drive gear 22, the drive gear 22 rotates left and right, thereby driving the rotating rod 23 to rotate. This causes the rotating rod 23 to drive the two ends of the placement block 24 to swing up and down in a fan shape, so that the templates inside the upper heating module 4 and the lower heating module 5 can shake up and down, making the shaking of the templates more uniform.

[0027] Temperature sensors are fixedly installed on the inner walls of both the upper heating module 4 and the lower heating module 5 to precisely control the heating temperature.

[0028] A slider 17 is fixedly connected to the top of the fixed plate 6, and a limit rod 11 is fixedly connected to one side of the fixed plate 6. The bottom of the oscillating housing 2 is provided with an elongated groove, and the top of the slider 17 is slidably connected to the inside of the elongated groove; Two limiting plates 13 are fixedly connected to one side of the fixed plate 6 above the limiting rod 11.

[0029] A sliding groove is provided on one side of the swing rod 10, and one end of the centrifugal rod 9 is movably connected inside the sliding groove; One end of the limiting rod 11 is rotatably connected to one side of the swing rod 10.

[0030] The movable rod 14 is movably connected between the two limiting plates 13, and both ends of the movable rod 14 extend through the two limiting plates 13 respectively. Two oscillating springs 15 are fixedly connected between the two ends of the two moving rods 14 and the two limiting plates 13.

[0031] The interior of the oscillating housing 2 is divided into a placement space and a driving space by a partition plate; The shaking device is located inside the drive space, and the upper heating module 4 and the lower heating module 5 are located inside the placement space.

[0032] One end of the linkage rod 19 is movably connected to a linkage slider, and one end of the linkage slider is slidably connected to the inner wall of the sliding track 20.

[0033] One end of the linkage rod 19 drives the linkage slider to slide on the inner wall of the sliding track 20, so that the linkage rod 19 pushes the rack 21 to move back and forth on the partition plate through the sliding track 20.

[0034] The rack 21 and the sliding track 20 are arranged in a cross shape, and a connecting groove is provided on one side of the partition plate; One side of the rack 21 is slidably connected inside the connecting groove.

[0035] The placement block 24 is semi-cylindrical, and a threaded push rod 25 is threadedly connected to one side of the placement block 24; One end of the threaded push rod 25 passes through the placement block 24 and is rotatably connected to the push block 26.

[0036] By placing the upper heating module 4 and the lower heating module 5 inside the placement block 24, and then through the threaded connection between the placement block 24 and the threaded push rod 25, one end of the threaded push rod 25 pushes the push block 26 to clamp and fix the lower heating module 5, thereby fixing the lower heating module 5 inside the placement block 24.

[0037] Working principle: The operator places the -80℃ frozen sample horizontally on the sample support frame inside the lower heating module 5, and then closes the upper heating module 4 and the lower heating module 5. This creates a cylindrical heating cavity when the upper heating module 4 and the lower heating module 5 are closed, thanks to their semi-cylindrical shape. This allows the sample to be tightly wrapped, and the upper heating module 4 and the lower heating module 5 to heat the sample uniformly in all directions at 360°. Then, the first servo motor 7 is started by the control console 3, so that the moving end of the first servo motor 7 drives the drive turntable 8 to rotate, so that the centrifugal rod 9 moves in a circle around the center of the drive turntable 8. At the same time, one end of the centrifugal rod 9 slides in the sliding groove of the swing rod 10, so that the swing rod 10 drives the half gear 12 to swing left and right around the limit rod 11. Furthermore, through the meshing connection between the half gear 12 and the moving rod 14 (the middle part of the moving rod 14 is toothed), the half gear 12 drives the moving rod 14 to swing left and right. The moving rod 14 drives the oscillating outer casing 2 to swing left and right through the two connecting plates 16, which causes the upper heating module 4 and the lower heating module 5 to drive the sample to swing left and right. In addition, the shaking device can make the sample swing up and down in a fan shape. This combination of orthogonal oscillating motion forms a violent and uniform vortex and shear force inside the sample, which can instantly break the melting ice crystals and promote the rapid uniformity of temperature and composition in all parts of the sample. This allows the ice crystals to melt rapidly and the contents to mix evenly under the accelerated heat conduction of the upper heating module 4 and the lower heating module 5. The device can then be set via the touch screen on the control panel 3 so that after 2 minutes of shaking in the outer shell, the device will quickly shake the outer shell and automatically cool it down to 37°C within 2 minutes. It will then maintain the temperature at 37°C for the set time, at which point the device will automatically stop and issue a prompt. The operator can then take out the resuscitated, appropriately sized, and evenly mixed sample for direct use.

[0038] The above are merely preferred embodiments of the present invention and do not limit the scope of the patent. All equivalent changes and modifications made within the scope of the present invention should still fall within the scope of the present invention.

Claims

1. A dual-module oscillating resuscitation device for large-volume frozen biological agents, characterized in that: It includes a fixed base (1), the top of which is movably connected to an oscillating housing (2), and a control console (3) is fixedly connected to one side of the fixed base; The upper heating module (4) is installed inside the oscillating housing (2), and the lower heating module (5) is connected to the bottom of the upper heating module (4) by a hinge. Both the upper heating module (4) and the lower heating module (5) are semi-cylindrical and made of Peltier thermostatic metal blocks. Temperature sensors are fixedly installed on the inner walls of both modules. A fixed plate (6) is fixedly connected to the bottom inner wall of the fixed base (1), and a first servo motor (7) is fixedly connected to one side of the fixed plate (6). The movable end of the first servo motor (7) passes through the fixed plate (6) and is fixedly connected to a drive turntable (8). A centrifugal rod (9) is fixedly connected to one side of the drive turntable (8), and a swing rod (10) is movably connected to one end of the centrifugal rod (9). A half gear (12) is fixedly connected to one end of the swing rod (10), and a moving rod (14) is meshed with one side of the half gear (12). Both ends of the moving rod (14) are fixedly connected to connecting plates (16), and the tops of the two connecting plates (16) are fixedly connected to the bottom of the oscillating outer casing (2); The oscillating housing (2) is equipped with a shaking device for fan-shaped oscillation of the heating lower module (5).

2. The dual-module oscillating resuscitation device for large-volume frozen biological agents according to claim 1, characterized in that: The shaking device includes a second servo motor (18), a linkage rod (19), a sliding rail (20), a rack (21), a drive gear (22), a rotating rod (23), and a placement block (24). The movable end of the second servo motor (18) passes through the oscillating housing (2) and is fixedly connected to a linkage rod (19). One end of the linkage rod (19) is movably connected to a sliding rail (20). A rack (21) is fixedly connected to one side of the sliding track (20) via a fixing block. A drive gear (22) is meshed with the top of the rack (21). A rotating rod (23) is fixedly connected to the center of the drive gear (22). One end of the rotating rod (23) is fixedly connected to the placement block (24) through the partition plate.

3. The dual-module oscillating resuscitation device for large-volume frozen biological agents according to claim 1, characterized in that: Temperature sensors are fixedly installed on the inner walls of both the upper heating module (4) and the lower heating module (5) to precisely control the heating temperature.

4. The dual-module oscillating resuscitation device for large-volume frozen biological agents according to claim 1, characterized in that: A slider (17) is fixedly connected to the top of the fixed plate (6), and a limit rod (11) is fixedly connected to one side of the fixed plate (6). The bottom of the oscillating housing (2) is provided with an elongated groove, and the top of the slider (17) is slidably connected to the inside of the elongated groove; Two limiting plates (13) are fixedly connected to one side of the fixed plate (6) above the limiting rod (11).

5. A dual-module oscillating resuscitation device for large-volume frozen biological agents according to claim 1, characterized in that: A sliding groove is provided on one side of the swing rod (10), and one end of the centrifugal rod (9) is movably connected inside the sliding groove; One end of the limiting rod (11) is rotatably connected to one side of the swing rod (10).

6. A dual-module oscillating resuscitation device for large-volume frozen biological agents according to claim 1, characterized in that: The movable rod (14) is movably connected between the two limiting plates (13), and both ends of the movable rod (14) pass through the two limiting plates (13) respectively to extend; Two oscillating springs (15) are fixedly connected between the two ends of the two moving rods (14) and the two limiting plates (13).

7. A dual-module oscillating resuscitation device for large-volume frozen biological agents according to claim 1, characterized in that: The interior of the oscillating housing (2) is divided into a placement space and a driving space by a partition plate; The shaking device is located inside the drive space, and the upper heating module (4) and the lower heating module (5) are located inside the placement space.

8. A dual-module oscillating resuscitation device for large-volume frozen biological agents according to claim 2, characterized in that: One end of the linkage rod (19) is movably connected to the linkage slider, and one end of the linkage slider is slidably connected to the inner wall of the sliding track (20).

9. A dual-module oscillating resuscitation device for large-volume frozen biological agents according to claim 2, characterized in that: The rack (21) and the sliding track (20) are arranged in a cross shape, and a connecting groove is provided on one side of the partition plate; One side of the rack (21) is slidably connected inside the connecting groove.

10. A dual-module oscillating resuscitation device for large-volume frozen biological agents according to claim 2, characterized in that: The placement block (24) is semi-cylindrical, and a threaded push rod (25) is threadedly connected to one side of the placement block (24). One end of the threaded push rod (25) passes through the placement block (24) and is rotatably connected to the push block (26).

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