Automatic sampling device for embryo implantation screening

Abstract

The invention relates to the technical field of sampling devices, in particular to an automatic sampling device for embryo implantation screening, which comprises a treatment table, a treatment mechanism is mounted on the treatment table, the treatment mechanism comprises a shading cover moving on the treatment table, a sampling mechanism is integrated on the shading cover, an exhaust pipe is mounted in the shading cover, and an air outlet is formed in the shading cover. The device comprises a light shielding cover, a treatment table is arranged on the light shielding cover, a culture dish is mounted on the treatment table, an exhaust mechanism is movably arranged on one side of the light shielding cover, and the exhaust mechanism comprises a gas storage tank mounted on one side of the treatment table. When the exhaust plate moves above the first exhaust pipe, gas in the gas storage tank is conveyed into the exhaust pipe through the first exhaust pipe and the hose, so that low-oxygen mixed gas is sprayed out synchronously in the descending process, the gas environment of a sampling area is replaced before sealing, and the influence of the environment during sampling is reduced.

Description

Technical Field

[0001] This invention relates to the field of sampling device technology, specifically to an automated sampling device for embryo implantation screening. Background Technology

[0002] Preimplantation genetic screening (PGS) is a crucial step in assisted reproductive technology. By biopsiing and analyzing trophoblast cells, embryos with normal chromosomes or without specific genetic diseases can be selected for transfer, which can significantly improve clinical pregnancy rates and reduce miscarriage rates and the risk of birth defects. Currently, embryo biopsies mainly rely on embryologists to perform manual operations under an inverted microscope. The standard procedure usually includes: transferring the embryo from the incubator to the micromanipulation table, making an opening in the zona pellucida using an acidic liquid or laser, and then aspirating several trophoblast cells with a microinjection needle or biopsy needle. When using existing sampling devices, after the embryo leaves the incubator containing a low-oxygen mixture, it is exposed to laboratory air. The oxygen partial pressure in the air is much higher than that in the physiological environment, and the carbon dioxide concentration is insufficient, causing the pH of the culture medium to rise rapidly. At the same time, the temperature drops and the liquid evaporates. This environmental fluctuation will cause oxidative stress and metabolic interference to the embryo, which will seriously affect its developmental potential and survival rate after biopsy. Summary of the Invention

[0003] The purpose of this invention is to provide an automatic sampling device for embryo implantation screening. During the downward pressing of the light-shielding cover, the connecting plate synchronously drives the exhaust plate to move inside the gas storage box. When the exhaust plate moves above the first exhaust pipe, it transports the gas inside the gas storage box to the exhaust pipe through the first exhaust pipe and the hose, thereby simultaneously spraying out a low-oxygen mixture during the descent. Before sealing, the gas environment of the sampling area is replaced, reducing the impact of the environment during sampling.

[0004] To achieve the above objectives, the present invention provides the following technical solution: an automatic sampling device for embryo implantation screening, comprising a processing table, wherein a processing mechanism is installed on the processing table; The processing mechanism includes a light-shielding cover movable on the processing table, a sampling mechanism integrated on the light-shielding cover, an exhaust pipe installed inside the light-shielding cover, a petri dish installed on the processing table, and an exhaust mechanism movable on one side of the light-shielding cover. The exhaust mechanism includes an air storage box installed on one side of the processing table, an exhaust plate that slides inside the air storage box, a first exhaust pipe installed at one end of the air storage box, one end of the first exhaust pipe connected to an exhaust pipe, a second exhaust pipe installed at the bottom of the first exhaust pipe, and one end of the second exhaust pipe connected to a cleaning mechanism. The cleaning mechanism includes a working chamber located inside the processing table. A piston moves inside the working chamber, and a liquid storage cylinder is sleeved on the outside of the piston. One end of the working chamber is connected to a third exhaust pipe, and one side of the liquid storage cylinder is connected to a drain pipe. One end of the third exhaust pipe is connected to the drain pipe, and one end of the drain pipe is connected to the sampling mechanism.

[0005] Preferably, an electric telescopic rod is installed on the processing table, the output end of the electric telescopic rod is connected to the light-shielding cover, and a sealing groove is provided inside the processing table, the size of which is adapted to the light-shielding cover.

[0006] Preferably, the sampling mechanism includes a sampling tube installed on a light-shielding cover, one end of the drain pipe is connected to the sampling tube, a pressure valve is installed at the connection between the drain pipe and the sampling tube, an adsorption tube is installed on one side of the sampling tube, and a laser system and a microscopic imaging auxiliary component are integrated on the sampling mechanism.

[0007] Preferably, a connecting plate is fixed to one side of the light-shielding cover, one end of the connecting plate passes through the air storage box and is connected to the exhaust plate, and an air inlet pipe is opened at one end of the air storage box. The air inlet pipe is connected to an external air storage device, and a first one-way valve is installed inside the air inlet pipe.

[0008] Preferably, a second one-way valve is installed inside the first exhaust pipe, and a flexible hose is connected to one end of the first exhaust pipe, with one end of the flexible hose passing through a light-shielding cover and communicating with the exhaust pipe.

[0009] Preferably, the second exhaust pipe is located inside the air storage box and is positioned lower than the first exhaust pipe, with one end of the second exhaust pipe passing through the processing table and communicating with the working chamber.

[0010] Preferably, a spring is installed inside the liquid storage cylinder, one end of the spring is connected to the piston, the other end of the spring is connected to the inner wall of the liquid storage cylinder, and a feeding pipe is connected to one end of the liquid storage cylinder, with a third one-way valve installed inside the feeding pipe.

[0011] Preferably, a pressure valve is installed inside the third exhaust pipe, and a fourth one-way valve is installed at the connection between the drain pipe and the storage tank.

[0012] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention utilizes the process of the light-shielding cover being pressed down to synchronously drive the exhaust plate to move inside the gas storage box via the connecting plate. When the exhaust plate moves above the first exhaust pipe, it transports the gas inside the gas storage box to the exhaust pipe through the first exhaust pipe and the hose, thereby simultaneously spraying out a low-oxygen mixture during the descent. This replaces the gas environment of the sampling area before sealing, reducing the impact of the environment during sampling.

[0013] 2. This invention increases the air pressure inside the working chamber as the downward pressure distance increases, thereby driving the piston to compress the spring and discharge the sterile culture medium inside the spring through the drain pipe into the sampling tube. This cleans the inside of the sampling tube, diluting and removing any trace impurities, metabolic waste, or debris generated during previous operations that may be present in the sampling area. This further reduces the risk of any non-target substances being accidentally inhaled into the sampling tube, ensuring sample purity.

[0014] 3. In this invention, when the internal air pressure of the working chamber exceeds the threshold of the second one-way valve inside the third exhaust pipe, the low-oxygen mixed gas inside the working chamber is discharged through the third exhaust pipe, pushing the residual sterile culture medium inside the drain pipe through the sampling tube and spraying it into the culture dish, forming a turbulent gas-liquid mixed flow. This physically scrubs the inner wall of the sampling tube, improving the treatment effect of the sterile culture medium. At the same time, the sterile culture medium sprayed into the culture dish allows fresh, warm, and gas-saturated culture medium to be directly added before the sealing operation, immediately diluting metabolic waste, replenishing nutrients, and stabilizing pH and osmotic pressure. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a partial structural diagram of the processing mechanism of the present invention; Figure 3 This is a second schematic diagram of the processing mechanism of the present invention; Figure 4 This is one of the structural schematic diagrams of the exhaust mechanism of the present invention; Figure 5 This is a second schematic diagram of the exhaust mechanism of the present invention; Figure 6 For the present invention Figure 5 Enlarged view of section A in the middle; Figure 7 This is a third schematic diagram of the processing mechanism of the present invention.

[0016] In the diagram: 1. Processing table; 2. Processing mechanism; 21. Light shield; 22. Electric telescopic rod; 23. Sampling mechanism; 231. Sampling tube; 232. Adsorption tube; 24. Exhaust pipe; 25. Petri dish; 3. Exhaust mechanism; 31. Gas storage tank; 32. Connecting plate; 33. Air inlet pipe; 34. Second exhaust pipe; 35. First exhaust pipe; 36. Exhaust plate; 37. Hose; 4. Cleaning mechanism; 41. Working chamber; 42. Piston; 43. Liquid storage cylinder; 44. Feeding pipe; 45. Spring; 46. Third exhaust pipe; 47. Drain pipe. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0018] Figures 1 to 7 As shown, the present invention provides an automatic sampling device for embryo implantation screening, including a processing table 1, on which a processing mechanism 2 is installed; The processing mechanism 2 includes a light-shielding cover 21 that moves on the processing table 1, a sampling mechanism 23 integrated on the light-shielding cover 21, an exhaust pipe 24 installed inside the light-shielding cover 21, a petri dish 25 installed on the processing table 1, and an exhaust mechanism 3 that moves on one side of the light-shielding cover 21. The exhaust mechanism 3 includes an air storage box 31 installed on one side of the processing table 1. An exhaust plate 36 slides inside the air storage box 31. A first exhaust pipe 35 is installed at one end of the air storage box 31. One end of the first exhaust pipe 35 is connected to the exhaust pipe 24. A second exhaust pipe 34 is installed at the bottom of the first exhaust pipe 35. One end of the second exhaust pipe 34 is connected to the cleaning mechanism 4. The cleaning mechanism 4 includes a working chamber 41 located inside the processing table 1. A piston 42 moves inside the working chamber 41. A liquid storage cylinder 43 is sleeved on the outside of the piston 42. One end of the working chamber 41 is connected to a third exhaust pipe 46. One side of the liquid storage cylinder 43 is connected to a drain pipe 47. One end of the third exhaust pipe 46 is connected to the drain pipe 47. One end of the drain pipe 47 is connected to the sampling mechanism 23.

[0019] Before starting the work, the cultured embryos are placed inside the culture dish 25. At the same time, the electric telescopic rod 22 is activated to drive the light-shielding cover 21 to descend, so that the light-shielding cover 21 is inserted into the sealing groove inside the processing table 1, thereby sealing the sampling area and preventing the external environment from affecting the sampling. At the same time, the light-shielding cover 21 is designed with light-shielding material, thereby shielding the sampling area from light and improving the sampling accuracy.

[0020] Simultaneously, during the downward pressing of the light-shielding cover 21, the exhaust plate 36 is moved inside the gas storage box 31 through the connecting plate 32. When the exhaust plate 36 moves above the first exhaust pipe 35, it transports the gas inside the gas storage box 31 to the exhaust pipe 24 through the first exhaust pipe 35 and the hose 37, thereby simultaneously spraying out low-oxygen mixed gas during the descent, replacing the gas environment of the sampling area before sealing, and reducing the impact of the environment during sampling.

[0021] Simultaneously, after the light-shielding cover 21 is inserted into the sealing groove, the exhaust plate 36 moves to below the first exhaust pipe 35. At the same time, the electric telescopic rod 22 drives the light-shielding cover 21 to continue moving, and the low-oxygen mixture remaining in the gas storage box 31 is discharged into the working chamber 41 through the second exhaust pipe 34. It accumulates inside the working chamber 41. At the same time, as the downward pressure distance increases, the air pressure inside the working chamber 41 increases, thereby driving the piston 42 to compress the spring 45 and move it. The sterile culture medium inside the spring 45 is discharged into the sampling tube 231 through the drain pipe 47. The inside of the sampling tube 231 is cleaned, diluted and removed, and any trace impurities, metabolic waste or debris generated in the sampling area are removed. This further reduces the risk of any non-target substances being accidentally inhaled into the sampling tube and ensures the purity of the sample.

[0022] Simultaneously, as the piston 42 compresses the spring 45, the air pressure inside the working chamber 41 gradually increases and remains in balance with the elastic force of the spring 45. When the air pressure inside the working chamber 41 exceeds the threshold of the second one-way valve inside the third exhaust pipe 46, the low-oxygen mixture inside the working chamber 41 is discharged through the third exhaust pipe 46, pushing the residual sterile culture medium inside the drain pipe 47 through the sampling tube 231 and spraying it into the culture dish 25, forming a turbulent gas-liquid mixture flow. This physically scrubs the inner wall of the sampling tube 231, improving the treatment effect of the sterile culture medium. At the same time, the sterile culture medium sprayed into the culture dish 25 allows fresh, warm, and gas-saturated culture medium to be directly added before the sealing operation, immediately diluting metabolic waste, replenishing nutrients, stabilizing pH and osmotic pressure, improving the survival rate of embryos after sampling, and providing better buffering and protection for the embryos, reducing physical and thermal stress.

[0023] In an optional embodiment, an electric telescopic rod 22 is installed on the processing table 1, the output end of the electric telescopic rod 22 is connected to the light shield 21, and a sealing groove is provided inside the processing table 1, the size of which is adapted to the light shield 21.

[0024] It should be noted that when the electric telescopic rod 22 is activated, the light-shielding cover 21 is driven to descend, so that the light-shielding cover 21 is inserted into the sealing groove inside the processing table 1, thereby sealing the sampling area and preventing the external environment from affecting the sampling. At the same time, the light-shielding cover 21 is designed with light-shielding material, thereby shielding the sampling area from light and improving the sampling accuracy.

[0025] In an optional embodiment, the sampling mechanism 23 includes a sampling tube 231 mounted on a light-shielding cover 21, one end of a drain pipe 47 connected to the sampling tube 231, a pressure valve installed at the connection between the drain pipe 47 and the sampling tube 231, an adsorption tube 232 installed on one side of the sampling tube 231, and a laser system and a microscopic imaging auxiliary component integrated on the sampling mechanism 23.

[0026] It should be noted that at the start of sampling, the embryo inside the sealed cavity is imaged in high definition by a microscopic imaging auxiliary component, which automatically identifies the embryo's outline, zona pellucida, and the location of the trophoblast cell cluster. Based on the image, the system software automatically calculates the optimal laser drilling point and the insertion path of the sampling tube 231. The entire sampling mechanism 23 is moved above the target embryo, aligning the laser focus with the tip of the sampling tube 231 and the adsorption tube 232 with the calculated coordinates. The integrated laser system is then activated, emitting several to dozens of laser pulses to precisely etch a circular opening on the zona pellucida of the embryo. After the laser stops, the sampling mechanism 23 is precisely moved along the predetermined path, adsorbing the embryo cells through the adsorption tube 232. Simultaneously, the sampling tube 231 and its tip are slowly and smoothly inserted into the embryo through the laser opening, approaching the target trophoblast cells, and a certain number of cells are drawn up through the sampling tube 231.

[0027] In an optional embodiment, a connecting plate 32 is fixed to one side of the light-shielding cover 21. One end of the connecting plate 32 passes through the air storage box 31 and is connected to the exhaust plate 36. An air inlet pipe 33 is provided at one end of the air storage box 31. The air inlet pipe 33 is connected to an external air storage device. A first one-way valve is installed inside the air inlet pipe 33.

[0028] It should be noted that when the light-shielding cover 21 moves, it drives the exhaust plate 36 to move inside the air storage box 31 through the connecting plate 32, and replenishes the low-oxygen mixture inside the air storage box 31 through the air intake pipe 33.

[0029] In an optional embodiment, a second one-way valve is installed inside the first exhaust pipe 35, and a hose 37 is connected to one end of the first exhaust pipe 35. The hose 37 passes through the light shield 21 and is connected to the exhaust pipe 24.

[0030] It should be noted that during the downward pressing of the light-shielding cover 21, the exhaust plate 36 is moved inside the gas storage box 31 synchronously through the connecting plate 32. When the exhaust plate 36 moves above the first exhaust pipe 35, it transports the gas inside the gas storage box 31 to the exhaust pipe 24 through the first exhaust pipe 35 and the hose 37, thereby simultaneously spraying out low-oxygen mixed gas during the descent. Before sealing, the gas environment of the sampling area is replaced, reducing the impact of the environment during sampling. When the light-shielding cover 21 moves, the hose 37 moves with the light-shielding cover 21.

[0031] In an optional embodiment, the second exhaust pipe 34 is opened inside the air storage box 31 and is positioned lower than the first exhaust pipe 35. One end of the second exhaust pipe 34 passes through the processing table 1 and communicates with the working chamber 41.

[0032] It should be noted that after the light-shielding cover 21 is inserted into the sealing groove, the exhaust plate 36 moves to below the first exhaust pipe 35. At the same time, the electric telescopic rod 22 drives the light-shielding cover 21 to continue moving, and discharges the low-oxygen mixture remaining inside the gas storage box 31 into the working chamber 41 through the second exhaust pipe 34, where it accumulates inside the working chamber 41.

[0033] In an optional embodiment, a spring 45 is installed inside the liquid storage cylinder 43. One end of the spring 45 is connected to the piston 42, and the other end of the spring 45 is connected to the inner wall of the liquid storage cylinder 43. One end of the liquid storage cylinder 43 is connected to a feeding pipe 44, and a third one-way valve is installed inside the feeding pipe 44.

[0034] It should be noted that as the downward pressure increases, the air pressure inside the working chamber 41 increases, thereby driving the piston 42 to compress the spring 45 and move it. The sterile culture medium inside the spring 45 is discharged into the sampling tube 231 through the drain pipe 47, which cleans the inside of the sampling tube 231, dilutes and removes any trace impurities, metabolic waste or debris generated in the sampling area.

[0035] In an optional embodiment, a pressure valve is installed inside the third exhaust pipe 46, and a fourth check valve is installed at the connection between the drain pipe 47 and the storage tank 43.

[0036] It should be noted that as the piston 42 compresses the spring 45, the air pressure inside the working chamber 41 gradually increases and remains in balance with the elastic force of the spring 45. When the air pressure inside the working chamber 41 exceeds the threshold of the second one-way valve inside the third exhaust pipe 46, the low-oxygen mixture inside the working chamber 41 is discharged through the third exhaust pipe 46, pushing the residual sterile culture medium inside the drain pipe 47 through the sampling tube 231 and spraying it into the culture dish 25, forming a turbulent gas-liquid mixture flow. This physically scrubs the inner wall of the sampling tube 231, improving the treatment effect of the sterile culture medium. At the same time, the sterile culture medium sprayed into the culture dish 25 allows fresh, warm, and gas-saturated culture medium to be directly added before the sealing operation, immediately diluting metabolic waste and replenishing nutrients.

[0037] Working principle: Before starting the work, the cultured embryos are placed inside the culture dish 25. At the same time, the electric telescopic rod 22 is activated to drive the light-shielding cover 21 to descend, so that the light-shielding cover 21 is inserted into the sealing groove inside the processing table 1. The light-shielding cover 21 is designed with light-shielding material to shield the sampling area from light.

[0038] At the same time, during the downward pressing of the shading cover 21, the exhaust plate 36 is moved inside the gas storage box 31 through the connecting plate 32. When the exhaust plate 36 moves above the first exhaust pipe 35, it transports the gas inside the gas storage box 31 to the exhaust pipe 24 through the first exhaust pipe 35 and the hose 37, thereby simultaneously spraying out low-oxygen mixed gas during the descent.

[0039] Simultaneously, after the light-shielding cover 21 is inserted into the sealing groove, the exhaust plate 36 moves to below the first exhaust pipe 35. At the same time, the electric telescopic rod 22 drives the light-shielding cover 21 to continue moving, and the low-oxygen mixture remaining in the gas storage box 31 is discharged into the working chamber 41 through the second exhaust pipe 34, where it accumulates. As the downward pressure distance increases, the gas pressure inside the working chamber 41 increases, thereby driving the piston 42 to compress the spring 45 and move it. The sterile culture medium inside the spring 45 is discharged into the sampling tube 231 through the drain pipe 47, and the inside of the sampling tube 231 is cleaned.

[0040] Simultaneously, as the piston 42 compresses the spring 45, the air pressure inside the working chamber 41 gradually increases and remains in balance with the elastic force of the spring 45. When the air pressure inside the working chamber 41 exceeds the threshold of the second one-way valve inside the third exhaust pipe 46, the low-oxygen mixture inside the working chamber 41 is discharged through the third exhaust pipe 46, pushing the residual sterile culture medium inside the drain pipe 47 to be sprayed out through the sampling tube 231 into the culture dish 25, forming a turbulent gas-liquid mixture flow, which physically scrubs the inner wall of the sampling tube 231, improving the treatment effect of the sterile culture medium, while the sterile culture medium is sprayed out into the culture dish 25.

[0041] After sealing, the embryo inside the sealed cavity is imaged in high definition using a microscopic imaging auxiliary component. The embryo's outline, zona pellucida, and the location of the trophoblast cell cluster are automatically identified. Based on the image, the system software automatically calculates the optimal laser drilling point and the insertion path of the sampling tube 231. The entire sampling mechanism 23 is moved above the target embryo, aligning the laser focus, the sampling tube 231, and the tip of the adsorption tube 232 with the calculated coordinates. The integrated laser system is then activated, emitting several to dozens of laser pulses to precisely etch a circular opening on the zona pellucida of the embryo. After the laser stops, the sampling mechanism 23 is precisely moved along a predetermined path, adsorbing embryonic cells through the adsorption tube 232. Simultaneously, the sampling tube 231 and its tip are inserted slowly and smoothly into the embryo through the laser opening, approaching the target trophoblast cells. A certain number of cells are then drawn up through the sampling tube 231 for sampling.

[0042] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An automated sampling device for embryo implantation screening, comprising a processing table (1), characterized in that, The processing station (1) is equipped with a processing mechanism (2); The processing mechanism (2) includes a light-shielding cover (21) that moves on the processing table (1), a sampling mechanism (23) integrated on the light-shielding cover (21), an exhaust pipe (24) installed inside the light-shielding cover (21), a petri dish (25) installed on the processing table (1), and an exhaust mechanism (3) that moves on one side of the light-shielding cover (21). The exhaust mechanism (3) includes an air storage box (31) installed on one side of the processing table (1), an exhaust plate (36) sliding inside the air storage box (31), a first exhaust pipe (35) installed at one end of the air storage box (31), one end of the first exhaust pipe (35) being connected to an exhaust pipe (24), a second exhaust pipe (34) installed at the bottom of the first exhaust pipe (35), and one end of the second exhaust pipe (34) being connected to a cleaning mechanism (4). The cleaning mechanism (4) includes a working chamber (41) inside the processing table (1), a piston (42) moving inside the working chamber (41), a liquid storage cylinder (43) sleeved on the outside of the piston (42), a third exhaust pipe (46) connected to one end of the working chamber (41), a drain pipe (47) connected to one side of the liquid storage cylinder (43), a third exhaust pipe (46) connected to the drain pipe (47) at one end, and a drain pipe (47) connected to the sampling mechanism (23) at one end.

2. The automated sampling device for embryo implantation screening according to claim 1, characterized in that, An electric telescopic rod (22) is installed on the processing table (1). The output end of the electric telescopic rod (22) is connected to the light shield (21). A sealing groove is provided inside the processing table (1). The size of the sealing groove is adapted to the light shield (21).

3. The automated sampling device for embryo implantation screening according to claim 1, characterized in that, The sampling mechanism (23) includes a sampling tube (231) installed on a light-shielding cover (21), one end of a drain pipe (47) is connected to the sampling tube (231), a pressure valve is installed at the connection between the drain pipe (47) and the sampling tube (231), an adsorption tube (232) is installed on one side of the sampling tube (231), and a laser system and a microscopic imaging auxiliary component are integrated on the sampling mechanism (23).

4. The automated sampling device for embryo implantation screening according to claim 1, characterized in that, A connecting plate (32) is fixed on one side of the light-shielding cover (21). One end of the connecting plate (32) passes through the gas storage box (31) and is connected to the exhaust plate (36). An air inlet pipe (33) is opened at one end of the gas storage box (31). The air inlet pipe (33) is connected to an external gas storage device. A first one-way valve is installed inside the air inlet pipe (33).

5. An automated sampling device for embryo implantation screening according to claim 1, characterized in that, The first exhaust pipe (35) is equipped with a second one-way valve. One end of the first exhaust pipe (35) is connected to a hose (37). One end of the hose (37) passes through the light shield (21) and is connected to the exhaust pipe (24).

6. An automated sampling device for embryo implantation screening according to claim 5, characterized in that, The second exhaust pipe (34) is located inside the gas storage box (31) and is positioned lower than the first exhaust pipe (35). One end of the second exhaust pipe (34) passes through the processing table (1) and communicates with the working chamber (41).

7. An automated sampling device for embryo implantation screening according to claim 1, characterized in that, A spring (45) is installed inside the liquid storage cylinder (43). One end of the spring (45) is connected to the piston (42), and the other end of the spring (45) is connected to the inner wall of the liquid storage cylinder (43). One end of the liquid storage cylinder (43) is connected to a feeding pipe (44), and a third one-way valve is installed inside the feeding pipe (44).

8. An automated sampling device for embryo implantation screening according to claim 1, characterized in that, A pressure valve is installed inside the third exhaust pipe (46), and a fourth check valve is installed at the connection between the drain pipe (47) and the storage cylinder (43).

Images