Identification method of target component of QingNing powder

Abstract

The invention discloses a method for identifying target components of QingNing powder. The method comprises the following steps: step 1, ensuring that a finished product contains three medicinal materials including honey white mulberry root-bark, wine-fried red poria cocos and fried semen lepidii from the level of microscopic identification; 2, 1-2 g of the product is taken, an extraction solvent is added, a corresponding extraction method is selected, filtration is performed, filtrate is evaporated to dryness, 1-2 ml of a dissolving agent is added to residues to dissolve the residues to serve as a test solution, reference medicinal materials are taken and prepared into a reference medicinal material solution with the method the same as that in the step 2, and 1-5 microliters of the test solution and 1-5 microliters of the reference medicinal material solution are sucked according to a thin-layer chromatography test; according to the method for identifying the target component of the Qingning powder, identification is carried out through thin-layer chromatography, complex data processing is not needed, existence of one or more characteristic components can be visually and specifically confirmed, interference caused by mixture signal superposition in a spectrum method is avoided, and different components in a complex sample can be clearly distinguished.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical identification technology, specifically a method for identifying the target components of Qingning Powder. Background Technology

[0002] Qingning Powder is a type of traditional Chinese medicine compound preparation (the formula varies slightly in different literature, but common ingredients include rhubarb, scutellaria, immature bitter orange, magnolia bark, and tangerine peel). The identification of the target components of Qingning Powder involves using modern analytical techniques to confirm the presence and content of characteristic components in the compound, thereby ensuring the authenticity, purity, and efficacy stability of the preparation. The existing technology is authorized under publication number CN113010613. Patent A discloses a multi-dimensional intelligent identification system and method for inspecting food and drugs, including a terminal system, a communication system, and a cloud computing service system. The terminal system mainly includes a high-definition camera and an infrared spectrometer, used to scan images of the biometric features and component spectra of the inspected food and drugs. While the device can detect components in food or drugs through the infrared spectrometer via the communication system and cloud computing service system, the infrared spectrometer obtains vibrational absorption spectra of molecular chemical bonds and functional groups. For complex mixtures (such as traditional Chinese medicine and compound foods), the spectrum is a superposition of signals from all components, which can affect the identification effect. Furthermore, the intensity of the infrared signal is related to the component content; if the content of a certain characteristic component is very low (<1%), its signal may be completely overwhelmed by the strong signal of the main components (such as starch and excipients), leading to missed detection. Therefore, we propose a method for identifying the target components of Qingning Powder. Summary of the Invention

[0003] The technical problem to be solved by this invention is to overcome the shortcomings of existing methods and provide a method for identifying the target components of Qingning Powder. The method uses thin-layer chromatography for identification, which does not require complex data processing. It allows for direct visual comparison of spots of the same position and color / fluorescence as the test sample and the reference sample. It can very intuitively and specifically confirm the presence of one or more characteristic components. Moreover, the physical separation of components is achieved first in the identification process, avoiding the interference of mixed signal superposition in spectroscopic methods. It can clearly distinguish different components in complex samples and effectively solve the problems in the background technology.

[0004] To achieve the above objectives, the present invention provides the following technical solution: a method for identifying the target components of Qingning Powder, comprising the following steps;

[0005] Step 1: From a microscopic identification perspective, ensure that the finished product contains three medicinal materials: honey-processed mulberry bark, wine-fried red poria cocos, and fried lepidium seed.

[0006] Step 2: Take 1-2g of this product, add extraction solvent, select the corresponding extraction method, filter, evaporate the filtrate to dryness, dissolve the residue in 1-2ml of solvent to prepare the test solution. Take the reference medicinal material separately and prepare the reference medicinal material solution using the same method as in Step 2. Perform the test according to the thin-layer chromatography method. Take 1-5ul of the test solution and 1-5ul of the reference medicinal material solution, spot them separately on the same stationary phase, then select the corresponding developing solvent, develop, remove, air dry, spray with positioning reagent, heat at 105℃ until the spots are clearly visible, and then examine according to the corresponding detection method. In the chromatogram of the test sample, a main spot of the same color appears at the corresponding position as in the chromatogram of the reference medicinal material. Identification is performed by thin-layer chromatography, which does not require complex data processing. The test sample and the reference sample can be directly visually compared with the spots of the same position and the same color / fluorescence. It can very intuitively and specifically confirm the presence of one or more characteristic components. Moreover, the physical separation of components is achieved first in the identification process, avoiding the interference of mixed signal superposition in the spectroscopic method, and can clearly distinguish different components in complex samples.

[0007] Furthermore, 1g of this product is taken, 25ml of methanol is added, and the mixture is sonicated for 30 minutes. The mixture is then filtered, the filtrate is evaporated to dryness, and the residue is dissolved in 2ml of methanol to prepare the test solution. Separately, 1g of mulberry bark reference material is taken and a reference material solution is prepared using the same method. The test is performed by thin-layer chromatography. 4ul of each of the above two solutions is applied separately to the same silica gel G thin-layer plate. Petroleum ether (60-90℃)-toluene-chloroform (2:1:2) is used as the developing solvent. The plate is developed, removed, and dried. It is then sprayed with 5% vanillin-sulfuric acid solution and heated at 105℃ until the spots are clearly visible. In the chromatogram of the test sample, a main spot of the same color appears at the corresponding position as in the chromatogram of the reference material, thus enabling identification using mulberry bark as a reference.

[0008] Furthermore, 1g of the sample is taken, 20ml of 70% methanol is added, the mixture is heated under reflux for 1 hour, filtered, the filtrate is evaporated to dryness, and the residue is dissolved in 2ml of methanol to prepare the test solution. Quercetin-3-OBD-glucose-7-O-β-D-gentiopicroside reference standard is prepared by adding 70% methanol to a solution containing 90μg per ml, which is used as the reference solution. Thin-layer chromatography is performed by applying 1μl of each of the two solutions to the same polyamide film, using ethyl acetate-methanol-water (7:4:1) as the developing solvent. After development, the film is removed, dried, and sprayed with 2% aluminum trichloride ethanol solution. The fluorescent spots are identical when heated under hot air. The heating temperature range during reflux is 80-100℃. This method allows for identification using quercetin-3-OBD-glucose-7-O-β-D-gentiopicroside as a reference standard.

[0009] Furthermore, 1g of the sample is taken, 50ml of ether is added, and the mixture is sonicated for 10 minutes. The mixture is then filtered, the filtrate is evaporated to dryness, and the residue is dissolved in 1ml of methanol to prepare the test solution. 1g of Poria cocos reference material is also taken and prepared as a reference solution using the same method. Thin-layer chromatography is performed, with 4ul of the test solution and 3μl of the reference solution applied separately to the same silica gel G thin-layer plate. The plate is developed using toluene-ethyl acetate-formic acid (20:5:0.5) as the developing solvent. After development, the plate is removed, dried, and sprayed with a 2% vanillin-sulfuric acid solution-ethanol (4:1) mixture. The plate is then heated at 105°C until the spots are clearly visible. In the chromatogram of the test sample, spots of the same color appear at the corresponding positions as in the chromatogram of the reference material, thus enabling identification using Poria cocos as a reference.

[0010] Furthermore, the stationary phase can be a silica gel GF1sa thin-layer plate or a silica gel G thin-layer plate prepared with 1% sodium hydroxide solution, which can separate the mixed target components from impurities through the difference in interaction with different components, providing a basis for subsequent qualitative identification and quantitative determination.

[0011] Furthermore, the developing solvent can be a lower layer solution of toluene-ethyl acetate-formic acid (20:5:0.5), ethyl acetate-methanol-formic acid-water (18:2:15:1), or chloroform-methanol-water (13:7:2) as the mobile phase. This mobile phase can carry the components in the sample spot upward on the stationary phase and achieve separation based on the difference in their affinity with the stationary phase and the mobile phase.

[0012] Furthermore, the detection method can be selected to examine under ultraviolet light (254nm) and under sunlight and ultraviolet light (365nm). It can accurately identify the target component spots by observing the differences in the color development characteristics of the components under different wavelength light sources, eliminate impurity interference, and ensure the accuracy and specificity of the identification results.

[0013] Furthermore, the product is prepared by mixing and pulverizing 222.42g of honey-processed mulberry bark, 222.42g of wine-fried red poria cocos, 110.32g of fried licorice root, 222.42g of fried plantain seed, and 222.42g of fried plantago seed into the finest powder, sieving, mixing, and then sterilizing at high temperature for 7-10 seconds at 160-180℃. After sieving and mixing, the desired product is obtained.

[0014] Furthermore, the product is a light brown to brown powder with a slightly fragrant odor and a slightly bitter and slightly sweet taste, and can be preliminarily identified through its physical appearance and sensory characteristics.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: The method for identifying the target components of Qingning Powder has the following advantages:

[0016] Identification by thin-layer chromatography requires no complex data processing. The test sample and the reference sample can be directly compared visually at the same position and with the same color / fluorescence. This method can very intuitively and specifically confirm the presence of one or more characteristic components. Moreover, the physical separation of components is achieved first during the identification process, avoiding the interference of mixed signal superposition in spectroscopic methods. It can clearly distinguish different components in complex samples. Attached Figure Description

[0017] Figure 1 This is a flowchart of a method for identifying the target components of Qingning Powder according to the present invention;

[0018] Figure 2 This is a partial flowchart of a method for identifying the target components of Qingning Powder according to the present invention. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Please see Figure 1-2 The present invention provides the following technical solutions:

[0021] Example 1: A method for identifying the target component of Qingning Powder, comprising the following steps:

[0022] Step 1: Scattered thick-walled cell groups containing stone cells, the cell lumens mostly contain square crystals (Mulberry bark); hyphae colorless or light brown, slender, slightly curved, branched, 3-8 μm in diameter, a few up to 16 μm (Stir-fried Poria cocos); seed coat outer epidermal cells are mucilage cells, square in cross-section, with thickened inner walls extending outward into cellulose columns, 8-18 μm long, with blunt, oblique, or truncate apexes, surrounded by mucilage textures. Seed coat inner epidermal cells are yellow, rectangular polygonal in surface view, 15-42 μm in diameter, with walls 5-8 μm thick (Stir-fried Job's tears).

[0023] Step 2: Take 1g of this product, add 25ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 2ml of methanol to prepare the test solution. Separately, take 1g of mulberry bark reference material and prepare a reference material solution using the same method. Perform thin-layer chromatography (Chinese Pharmacopoeia 2020 Edition, General Chapter 0502). Apply 4ul of each of the above two solutions to the same silica gel G thin-layer plate. Develop using petroleum ether (60-90℃)-toluene-chloroform (2:1:2) as the developing solvent. Remove the plate, air dry, spray with 5% vanillin-sulfuric acid solution, and heat at 105℃ until the spots are clearly visible. In the chromatogram of the test sample, a main spot of the same color appears at the corresponding position as in the chromatogram of the reference medicinal material. Ultrasonic mechanical treatment can be performed using an AS20500BD ultrasonic cleaner. Identification is performed by thin-layer chromatography, which does not require complex data processing. Spots of the same position and color / fluorescence of the test sample and the reference sample can be directly compared visually. This allows for very intuitive and specific confirmation of the presence of one or more characteristic components. Moreover, the physical separation of components is achieved first during the identification process, avoiding interference from the superposition of mixed signals in spectroscopic methods. It can clearly distinguish different components in complex samples.

[0024] This product is made by mixing and pulverizing 222.42g of honey-processed mulberry bark, 222.42g of wine-fried red poria cocos, 110.32g of fried licorice root, 222.42g of fried plantain seed, and 222.42g of fried plantago seed into the finest powder, sieving, mixing, and then sterilizing at high temperature (160-180℃) for 7-10 seconds. The steps of mixing, pulverizing, sieving, and mixing ensure a high degree of consistency in the composition of every component in every part of the product. Pulverizing to the finest powder greatly increases the contact area between the medicinal materials and the extraction solvent, ensuring that the target components can be quickly and completely dissolved during ultrasonic treatment or reflux heating. High-temperature instantaneous sterilization kills microorganisms, making the product meet hygiene standards.

[0025] The product is described as a light brown to brown powder with a slightly fragrant odor and a slightly bitter and slightly sweet taste. This is the legal description of the product's physical appearance and sensory characteristics, and it is the first and fastest quality inspection checkpoint. If the product is of normal quality, subsequent identification work will be carried out.

[0026] Example 2:

[0027] The difference between this embodiment and Embodiment 1 is that:

[0028] In this embodiment, step two: Take 1g of this product, add 20ml of 70% methanol, heat under reflux for 1 hour, filter, evaporate the filtrate to dryness, dissolve the residue in 2ml of methanol to obtain the test solution. Separately, take quercetin-3-OBD-glucose-7-O-β-D-gentiopicroside reference standard, add 70% methanol to prepare a solution containing 90μg per ml, as the reference solution. Perform thin-layer chromatography (Chinese Pharmacopoeia 2020 Edition, General Chapter 0502), take 1μl of each of the above two solutions, spot them separately on the same polyamide film, use ethyl acetate-methanol-water (7:4:1) as the developing solvent, develop, remove, air dry, spray with 2% aluminum trichloride ethanol solution, and heat the same fluorescent spots with hot air. The heating temperature range during reflux is 80-100℃.

[0029] Example 3:

[0030] The difference between this embodiment and Embodiment 1 is that:

[0031] In this embodiment, step two: Take 1g of this product, add 50ml of ether, sonicate for 10 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 1ml of methanol to obtain the test solution. Separately, take 1g of Poria cocos reference material and prepare a reference material solution using the same method. Perform thin-layer chromatography (Chinese Pharmacopoeia 2020 Edition, General Chapter 0502), applying 4ul of the test solution and 3μl of the reference material solution separately to the same silica gel G thin-layer plate. Develop using toluene-ethyl acetate-formic acid (20:5:0.5), remove, air dry, spray with a 2% vanillin-sulfuric acid solution-ethanol (4:1) mixture, and heat at 105°C until the spots are clearly visible. In the chromatogram of the test sample, spots of the same color appear at the corresponding positions as in the chromatogram of the reference material.

[0032] Example 4:

[0033] The difference between this embodiment and Embodiment 1 is that:

[0034] In this embodiment, step two: Take the test solution prepared in Example two as the test solution. Separately, take 0.5g of Plantago asiatica (Plantago) reference material, add 20ml of 70% methanol, heat under reflux for 1 hour, filter, evaporate the filtrate to dryness, and dissolve the residue in 2ml of methanol to obtain the reference material solution. Separately, take genipin glycoside reference standard and verbascoside reference standard, and prepare solutions containing 1mg each per 1ml with methanol to obtain the reference standard solutions. Perform thin-layer chromatography (Chinese Pharmacopoeia 2020 Edition, General Chapter 0502), taking 5μl each of the test solution and reference material solution, and 2μl of the reference standard solution, and spotting them separately on the same silica gel GF254 thin-layer plate. Use ethyl acetate-methanol-formic acid-water (18:2:15:1) as the developing solvent, develop, remove, air dry, and examine under ultraviolet light (254mm). In the chromatogram of the test sample, spots of the same color appear at the corresponding positions as in the chromatograms of the reference medicinal material and the reference standard.

[0035] Example 5:

[0036] The difference between this embodiment and Embodiment 1 is that:

[0037] In this embodiment, step two: Take 2g of this product, add 20ml of saturated sodium carbonate solution, sonicate for 20 minutes, centrifuge, adjust the pH of the filtrate to 1-2 with dilute hydrochloric acid, let stand for 30 minutes, centrifuge, extract the filtrate twice with ethyl acetate, 10ml each time, combine the ethyl acetate extracts, evaporate to dryness, dissolve the residue in 1ml of methanol, and use as the test solution. Separately take 0.5g of licorice (Glycyrrhiza uralensis) reference material and prepare a reference material solution using the same method. Separately take licorice back reference standard, add methanol to prepare a solution containing 1mg per ml, and use as the reference solution. Perform thin-layer chromatography (General Chapter 0502, Chinese Pharmacopoeia 2020 Edition). Apply 3 μL of the test solution, 1 μL each of the reference medicinal material solution and the reference standard solution to the same silica gel G thin-layer plate prepared with 1% sodium hydroxide solution. Develop using the lower layer of a chloroform-methanol-water (13:7:2) solution as the developing solvent. Remove the plate, air dry, spray with 10% sulfuric acid ethanol solution, and heat at 105℃ until the spots are clearly visible. Examine under sunlight and ultraviolet light at 365 nm. In the chromatogram of the test sample, spots or fluorescent spots of the same color should appear at the corresponding positions as in the chromatograms of the reference medicinal material and the reference standard.

[0038] The present invention provides a method for identifying the target components of Qingning Powder as follows: Before identification, 222.42g of honey-processed mulberry bark, 222.42g of wine-fried red poria cocos, 110.32g of fried licorice root, 222.42g of fried plantain seed, and 222.42g of fried plantago seed are mixed and pulverized into the finest powder, sieved, mixed evenly, and sterilized at high temperature for 7-10 seconds. After sieving and mixing, the desired product is obtained. Scattered thick-walled cell groups containing stone cells, the cell cavities mostly contain square crystals (honey mulberry bark); the hyphae are colorless or light brown, slender, slightly curved, branched, 3-8 μm in diameter, a few up to 16 μm (wine-fried red poria); the outer epidermal cells of the seed coat are mucilage cells, square in cross-section, with thickened inner walls extending outward into cellulose columns, 8-18 μm long, with blunt, oblique or truncate tips, and mucilage textures visible around them. The inner epidermal cells of the seed coat are yellow, rectangular in shape with a diameter of 15-42 μm and a wall thickness of 5-8 μm (from stir-fried coix seed). This microscopic identification ensures that the finished product contains the three medicinal materials: honey-processed mulberry bark, wine-fried red poria cocos, and stir-fried lepidium seed. Take 1-2 g of this product, add extraction solvent, select the corresponding extraction method, filter, and evaporate the filtrate to dryness at a temperature of 50-105℃. The final state is considered as "forming a dry, non-flowing powder, crystals, or solid film without visible liquid sheen or moisture" (water content below approximately 8%). Dissolve the residue in 1-2 ml of solvent to prepare the test solution. Take another reference medicinal material and prepare a reference medicinal material solution using the same method as in step two. Perform thin-layer chromatography, taking 1-5 μl of the test solution and 1-5 μl of the reference medicinal material solution, spotting them separately on the same stationary phase, then selecting the corresponding developing solvent, developing, removing, air-drying, spraying with positioning reagent, and heating at 105℃. Once the spots are clearly visible, examine them according to the corresponding detection method. In the chromatogram of the test sample, a main spot of the same color appears at the corresponding position as in the chromatogram of the reference medicinal material. Through thin-layer chromatography, compare the chromatographic spots of the test sample solution and the reference medicinal material solution. The test sample solution usually refers to the sample you are testing, such as Chinese herbal extracts, compound preparations, or raw medicinal materials. The reference medicinal material solution usually refers to a standard medicinal material with known qualified components and a clear source, such as a pharmacopoeia standard or reference material. This proves that the test sample contains the same main characteristic components as the reference medicinal material, thereby verifying the authenticity, consistency of components, or quality qualification of the test sample. The corresponding position refers to the same relative migration rate (Rf value) of the spots on the thin-layer plate. Rf value = distance from the center of the spot to the spot point ÷ distance from the front of the developing solvent to the spot point (when the experimental conditions are fixed, the Rf value of the same component is a fixed constant). This indicates that the polarity and adsorption-desorption characteristics of the component in the test sample and the reference medicinal material are consistent.Main spots of the same color refer to spots whose colors (such as yellow, red, fluorescent blue, etc.) are completely consistent. Specific components will show a fixed color under specific detection conditions (such as UV light irradiation and color development by colorimetric reagents) (for example, flavonoids show blue fluorescence under UV 365nm, and alkaloids show orange-red color when developed with potassium bismuth iodide). Consistent colors further confirm that the components are the same. "Main spots" refer to the core component spots with high content and strong clarity (excluding interference from trace impurity spots).

[0039] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for identifying the target components of Qingning Powder, characterized in that: Includes the following steps; Step 1: From a microscopic identification perspective, ensure that the finished product contains three medicinal materials: honey-processed mulberry bark, wine-fried red poria cocos, and fried lepidium seed. Step 2: Take 1-2g of this product, add extraction solvent, select the corresponding extraction method, filter, evaporate the filtrate to dryness, dissolve the residue in 1-2ml of solvent to prepare the test solution. Take the reference medicinal material separately and prepare the reference medicinal material solution using the same method as in Step 2. Perform the test according to the thin-layer chromatography method. Take 1-5ul of the test solution and 1-5ul of the reference medicinal material solution and spot them separately on the same stationary phase. Then select the corresponding developing solvent, develop, remove, air dry, spray with positioning reagent, and heat at 105℃ until the spots are clearly visible. Then examine according to the corresponding detection method. In the chromatogram of the test sample, a main spot of the same color appears at the corresponding position as in the chromatogram of the reference medicinal material.

2. The method for identifying the target component of Qingning Powder according to claim 1, characterized in that: Take 1g of this product, add 25ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 2ml of methanol to prepare the test solution. Take 1g of mulberry bark reference material and prepare a reference material solution using the same method. Perform thin-layer chromatography test, take 4ul of each of the above two solutions, and spot them separately on the same silica gel G thin-layer plate. Use petroleum ether (60-90℃)-toluene-chloroform (2:1:2) as the developing solvent, develop, remove, air dry, spray with 5% vanillin sulfuric acid solution, and heat at 105℃ until the spots are clearly visible. In the chromatogram of the test sample, a main spot of the same color appears at the corresponding position as in the chromatogram of the reference material.

3. The method for identifying the target component of Qingning Powder according to claim 1, characterized in that: Take 1g of this product, add 20ml of 70% methanol, heat under reflux for 1 hour, filter, evaporate the filtrate to dryness, dissolve the residue in 2ml of methanol to obtain the test solution. Separately, take quercetin-3-OBD-glucose-7-O-β-D-gentiopicroside reference standard, add 70% methanol to prepare a solution containing 90μg per ml to obtain the reference solution. Perform thin-layer chromatography test, take 1μl of each of the above two solutions, spot them separately on the same polyamide film, use ethyl acetate-methanol-water (7:4:1) as the developing solvent, develop, remove, air dry, spray with 2% aluminum trichloride ethanol solution, hot air to obtain the same fluorescent spots, the heating temperature range during reflux is 80-100℃.

4. The method for identifying the target component of Qingning Powder according to claim 1, characterized in that: Take 1g of this product, add 50ml of ether, sonicate for 10 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 1ml of methanol to prepare the test solution. Take 1g of Poria cocos reference material and prepare the reference material solution in the same way. Perform thin-layer chromatography test, take 4ul of the test solution and 3μl of the reference material solution, and spot them separately on the same silica gel G thin-layer plate. Use toluene-ethyl acetate-formic acid (20:5:0.5) as the developing solvent, develop, remove, dry, spray with 2% vanillin-sulfuric acid solution-ethanol (4:1) mixed solution, heat at 105°C until the spots are clearly visible. In the chromatogram of the test sample, spots of the same color appear at the corresponding positions as in the chromatogram of the reference material.

5. The method for identifying the target component of Qingning Powder according to claim 1, characterized in that: The stationary phase can be a silica gel GF1sa thin-layer plate or a silica gel G thin-layer plate prepared with 1% sodium hydroxide solution.

6. The method for identifying the target component of Qingning Powder according to claim 1, characterized in that: The developing solvent can be a lower layer solution of toluene-ethyl acetate-formic acid (20:5:0.5), ethyl acetate-methanol-formic acid-water (18:2:15:1), or chloroform-methanol-water (13:7:2).

7. The method for identifying the target component of Qingning Powder according to claim 1, characterized in that: The detection method can be selected from inspection under ultraviolet light (254nm) and inspection under sunlight and ultraviolet light (365nm).

8. The method for identifying the target component of Qingning Powder according to claim 1, characterized in that: The product is prepared by mixing and pulverizing 222.42g of honey-processed mulberry bark, 222.42g of wine-fried red poria cocos, 110.32g of fried licorice root, 222.42g of fried plantain seed, and 222.42g of fried plantago seed into the finest powder, sieving, mixing, and then sterilizing at high temperature for 7-10 seconds at 160-180℃.

9. The method for identifying the target component of Qingning Powder according to claim 1, characterized in that: The product is a light brown to brown powder with a slightly fragrant odor and a slightly bitter and slightly sweet taste.

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