Nano suspension containing vitamin minerals and preparation method thereof

Abstract

The invention provides a vitamin mineral nano suspension, which is prepared from the following raw materials in parts by weight: 10 to 50 parts of nutrient supplement, 0.1 to 2 parts of thickening agent, 1 to 6 parts of emulsifier, 0.1 to 5 parts of charge stabilizer and the balance of water through high-speed shearing and high-pressure homogenization on the basis of 100 parts of nano suspension, the charge stabilizer comprises at least one of tremella polysaccharide, zymosan and sodium chondroitin sulfate. The vitamin mineral nano suspension provided by the invention is subjected to high-pressure homogenization, so that the particle size of insoluble mineral components in the suspension can be remarkably reduced, the aggregation phenomenon of mineral particles treated by a high-pressure homogenization process is remarkably improved, the dispersion uniformity of the mineral particles is improved, and the stability of a product is enhanced while the palatability is improved. After the polysaccharide raw material is introduced, the aggregation phenomenon of the suspension after high-pressure homogenization can be further improved. In addition, by detecting and comparing the content change of vitamins and minerals, the nano suspension nutrient has high stability.

Description

Technical Field

[0001] This invention belongs to the field of suspension preparation technology, specifically relating to a nano-suspension containing vitamins and minerals and its preparation method. Background Technology

[0002] Currently, oral vitamin and mineral supplements are mainly available in tablet, powder, and capsule forms. However, with increasing health awareness and emphasis on nutritional supplementation, the market for liquid oral preparations is gradually expanding. Liquid formulations typically have a better taste and are easier to swallow, making them particularly suitable for children, the elderly, and patients with swallowing difficulties. This improves patient compliance and ensures adequate nutrient intake. Most vitamin and mineral products on the market are often bulky, causing chewing difficulties, swallowing problems, and difficulty in consumption for the elderly and children, often accompanied by unpleasant taste. Calcium supplements are a prime example, with calcium sources often primarily being calcium carbonate, which has low bioavailability and is not gentle enough on the stomach. Liquid vitamin and mineral preparations not only solve the problems of inconvenience and swallowing difficulties but also have good dispersibility in the gastrointestinal tract, a large surface area, and can quickly dissolve and release nutrients, making them easier for the intestines to absorb. They offer advantages such as high bioavailability, rapid absorption, and good taste.

[0003] However, some minerals have low solubility in water, and fat-soluble vitamins are insoluble in water, which can affect product quality and the absorption and utilization of nutrients. Quality control of liquid suspensions is also relatively complex. Currently, most liquid formulations on the market use thickeners combined with high-speed shearing to suspend insoluble mineral raw materials in water to ensure they do not settle or separate during shelf life. However, this method produces larger mineral particles, with particle sizes generally exceeding 1μm, resulting in poor dispersion uniformity and poor palatability when consumed. Fat-soluble vitamins, on the other hand, use emulsifiers to solubilize them and ensure the stability of vitamins in liquid formulations. However, emulsion droplets are easily affected by various factors and can break down, causing a significant decrease in vitamin content.

[0004] Therefore, providing a nano-suspension containing vitamins and minerals with small particle size, high dispersion uniformity, good stability of active ingredients, and good palatability has become a problem that needs to be solved. Summary of the Invention

[0005] In view of this, the technical problem to be solved by the present invention is to provide a nano-suspension containing vitamins and minerals and a method for preparing the same. The nano-suspension containing vitamins and minerals provided by the present invention has small particle size, high dispersion uniformity, good stability of active ingredients, and good palatability.

[0006] This invention provides a vitamin and mineral nano-suspension, which, based on 100 parts by weight of nano-suspension, is obtained by high-speed shearing and high-pressure homogenization of the following raw materials:

[0007] 10-50 parts nutrient supplement, 0.1-2 parts thickener, 1-6 parts emulsifier, 0.1-5 parts charge stabilizer, and the remainder is water;

[0008] The charge stabilizer includes at least one of Tremella polysaccharide, yeast polysaccharide, and sodium chondroitin sulfate.

[0009] Preferably, the nutrient supplement includes one or more of the following: vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, vitamin K, calcium salts, magnesium salts, zinc salts, iron salts, and selenium-enriched yeast.

[0010] Preferably, the thickener includes at least one of xanthan gum, carrageenan, pectin, guar gum, locust bean gum, and sodium carboxymethyl cellulose.

[0011] Preferably, the emulsifier includes at least one of gum arabic, mono- and diglycerides of fatty acids, phospholipids, monoglycerides of succinate, and sucrose fatty acid esters.

[0012] Preferably, it also includes an acidity regulator;

[0013] The acidity regulator includes at least one of citric acid, DL-malic acid, fumaric acid, sodium citrate, potassium citrate, lactic acid, and sodium bicarbonate.

[0014] Based on 100 parts by weight of nano suspension, it includes 0.1-3 parts of acidity regulator.

[0015] Preferably, it also includes sweeteners;

[0016] The sweetener is selected from at least one of sorbitol, sorbitol liquid, xylitol, erythritol, maltitol, maltitol liquid, isomaltitol, isomaltulose, mannitol, sucrose, trehalose, glucose, mogrosides, sucralose, and steviol glycosides.

[0017] Based on 100 parts by weight of nano suspension, it includes 5-20 parts of sweetener.

[0018] Preferred ingredients also include prebiotics;

[0019] The prebiotic is selected from at least one of fructooligosaccharides, galactooligosaccharides, inulin, resistant dextrin, polydextrose, and isomaltooligosaccharides;

[0020] Based on 100 parts by weight of nano-suspension, it includes 2-20 parts of prebiotics.

[0021] Preferably, it also includes other synergistic ingredients, which are selected from at least one of yeast β-glucan, glucosamine, zinc-enriched edible yeast powder, yeast extract, and glutathione yeast powder;

[0022] Based on 100 parts by weight of nano-suspension, it includes 0.1-10 parts of other synergistic ingredients.

[0023] This invention also provides a method for preparing the above-mentioned vitamin and mineral nanosuspension, comprising the following steps:

[0024] A) Mix the thickener, emulsifier, and water, heat and stir to obtain a mixed solution;

[0025] B) After cooling the mixed solution, add the nutrient supplement and perform high-speed shearing, then add the charge stabilizer, mix and stir, and filter to obtain the mixture.

[0026] C) The mixture is homogenized under high pressure, then filled and sterilized to obtain a vitamin and mineral nano suspension.

[0027] Preferably, in step A), the stirring speed is 500-1000 rpm;

[0028] In step B), the high-speed shearing speed is 2000-9000 rpm, and the time is 5-20 min;

[0029] In step C), the pressure of the high-pressure homogenization is 40~70MPa, the number of cycles is 2-5, and the temperature during the high-pressure homogenization process is always controlled to be below 40℃.

[0030] Compared with existing technologies, this invention provides a vitamin and mineral nano-suspension, which, based on 100 parts by weight of the nano-suspension, is obtained by high-speed shearing and high-pressure homogenization of the following raw materials: 10-50 parts of nutrient supplement, 0.1-2 parts of thickener, 1-6 parts of emulsifier, 0.1-5 parts of charge stabilizer, and the remainder being water; the charge stabilizer includes at least one of Tremella fuciformis polysaccharide, yeast polysaccharide, and sodium chondroitin sulfate. The vitamin and mineral nano-suspension provided by this invention, after high-pressure homogenization, can significantly reduce the particle size of insoluble mineral components in the suspension, significantly improve the aggregation phenomenon of mineral particles after high-pressure homogenization, improve its dispersion uniformity, enhance palatability, and strengthen product stability. The introduction of polysaccharide raw materials can further improve the aggregation phenomenon of the suspension after high-pressure homogenization. Furthermore, by detecting and comparing changes in vitamin and mineral content, this nano-suspension exhibits high nutrient stability. Attached Figure Description

[0031] Figure 1 This refers to the microscopic state of the test substance. Detailed Implementation

[0032] This invention provides a vitamin and mineral nano-suspension, which, based on 100 parts by weight of nano-suspension, is obtained by high-speed shearing and high-pressure homogenization of the following raw materials:

[0033] 10-50 parts nutrient supplement, 0.1-2 parts thickener, 1-6 parts emulsifier, 0.1-5 parts charge stabilizer, and the remainder is water;

[0034] The charge stabilizer includes at least one of Tremella polysaccharide, yeast polysaccharide, and sodium chondroitin sulfate.

[0035] The raw materials for preparation, based on 100 parts by weight of nano-suspension, include 10-50 parts of nutrient supplements, which can be any value between 10, 15, 20, 25, 30, 35, 40, 45, 50, or 10-50 parts of nutrient supplements. The nutrient supplements include one or more of the following: vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, vitamin K, calcium salts, magnesium salts, zinc salts, iron salts, and selenium-enriched yeast.

[0036] Based on 100 parts by weight of the nano-suspension, the raw materials also include 0.1-2 parts of a thickener, which can be any value between 0.1, 0.3, 0.5, 0.7, 0.9, 1.0, 1.1, 1.3, 1.5, 1.7, 1.9, 2.0, or 0.1-2 parts. The thickener includes at least one selected from xanthan gum, carrageenan, pectin, guar gum, locust bean gum, and sodium carboxymethyl cellulose.

[0037] Based on 100 parts by weight of the nano-suspension, the raw materials also include 1-6 parts of emulsifier, which can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, or any value between 1 and 6 parts. The emulsifier includes at least one selected from gum arabic, mono- and diglycerides of fatty acids, phospholipids, monoglycerides of succinate, and sucrose fatty acid esters.

[0038] Based on 100 parts by weight of the nano-suspension, the raw materials also include 0.1-5 parts of a charge stabilizer, which can be any value between 0.1, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, or 0.1-5 parts. The charge stabilizer includes at least one of Tremella fuciformis polysaccharide, yeast polysaccharide, and sodium chondroitin sulfate.

[0039] During high-pressure homogenization, mineral particles are crushed, resulting in a large number of fine mineral particles. The original suspension system cannot provide sufficient repulsive force to resist the attraction between particles, causing the increased fine particles after high-pressure homogenization to gradually aggregate and form a precipitate. This process becomes apparent over time. Adding a certain amount of polysaccharide raw materials imparts a higher charge density to the system through the groups on the sugar chains. By forming sufficient electrostatic repulsion and a certain steric hindrance effect, the system can regain stability.

[0040] In some preferred embodiments of the present invention, the raw materials for preparation, based on 100 parts by weight of the nano-suspension, further include 0.1-3 parts of an acidity regulator, which can be any value between 0.1, 0.5, 1, 1.5, 2, 2.5, 3, or 0.1-3 parts. The acidity regulator includes at least one of citric acid, DL-malic acid, fumaric acid, sodium citrate, potassium citrate, lactic acid, and sodium bicarbonate.

[0041] In some preferred embodiments of the present invention, the raw materials for preparation, based on 100 parts by weight of the nano-suspension, further include 5-20 parts of a sweetener, which can be 5, 7, 10, 12, 15, 17, 20, or any value between 5 and 20 parts. The sweetener is selected from at least one of sorbitol, sorbitol liquid, xylitol, erythritol, maltitol, maltitol liquid, isomaltitol, isomaltulose, mannitol, sucrose, trehalose, glucose, mogrosides, sucralose, and steviol glycosides.

[0042] In some preferred embodiments of the present invention, the raw materials for preparation, based on 100 parts by weight of nano-suspension, further include 2-20 parts of prebiotics, which can be 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, or any value between 2 and 20 parts. The prebiotics are selected from at least one of fructooligosaccharides, galactooligosaccharides, inulin, resistant dextrin, polydextrose, and isomaltooligosaccharides.

[0043] In some preferred embodiments of the present invention, the raw materials for preparation, based on 100 parts by weight of nano-suspension, further include 0.1-10 parts of other synergistic ingredients, which can be 0.1, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or any value between 0.1 and 10 parts. The other synergistic ingredients are selected from at least one of yeast β-glucan, glucosamine, zinc-enriched edible yeast powder, yeast extract, and glutathione yeast powder.

[0044] This invention also provides a method for preparing the above-mentioned vitamin and mineral nanosuspension, comprising the following steps:

[0045] A) Mix the thickener, emulsifier, and water, heat and stir to obtain a mixed solution;

[0046] B) After cooling the mixed solution, add the nutrient supplement and perform high-speed shearing, then add the charge stabilizer, mix and stir, and filter to obtain the mixture.

[0047] C) The mixture is homogenized under high pressure, then filled and sterilized to obtain a vitamin and mineral nano suspension.

[0048] Specifically, the present invention first prepares a mixed solution, wherein the thickener, emulsifier, and water are heated, mixed, and stirred together; the heating temperature is 60~80℃, and can be any value between 60, 65, 70, 75, 80, or 60~80℃. The stirring speed is 500-1000 rpm, and can be any value between 500, 600, 700, 800, 900, 1000, or 500-1000 rpm.

[0049] Alternatively, you can heat the water, add the thickener, start stirring to completely dissolve the thickener, and then add the emulsifier and stir.

[0050] The heating temperature is 60~80℃, which can be any value between 60, 65, 70, 75, 80, or 60~80℃.

[0051] The stirring speed is 500-1000 rpm, which can be any value between 500, 600, 700, 800, 900, 1000, or 500-1000 rpm.

[0052] In some specific embodiments of the present invention, if the ingredients include prebiotics and sweeteners, they can be added to the solution together with the emulsifier and stirred.

[0053] After stirring, a mixed solution is obtained. This solution is then cooled to room temperature, and the nutrient supplement is added and subjected to high-speed shearing to ensure uniform dispersion. The high-speed shearing speed is 2000-9000 rpm, which can be any value between 2000, 3000, 4000, 5000, 6000, 7000, 8000, or 9000 rpm, and the time is 5-20 minutes, which can be any value between 5, 10, 15, or 20 minutes.

[0054] In some specific embodiments of the present invention, if the ingredients include an acidity regulator, it can be subjected to high-speed shearing with nutrient supplements.

[0055] Next, a charge stabilizer is added, and the mixture is stirred and then filtered to obtain a final volumetric mixture. After stirring until dissolved, the mixture is brought to the required weight.

[0056] The material after being brought to a constant volume is passed through a 20-mesh filter to obtain a mixture.

[0057] The mixture is subjected to high-pressure homogenization. The pressure of the high-pressure homogenization is 40~70MPa, which can be any value between 40, 45, 50, 55, 60, 65, 70, or 40~70MPa. The number of cycles is 2-5, which can be 2, 3, 4, 5, or any value between 2 and 5. The temperature during the high-pressure homogenization process is controlled to always be below 40℃.

[0058] Finally, sterilization is performed after filling. This invention does not impose any special limitations on the sterilization method; any sterilization method known to those skilled in the art is acceptable.

[0059] This invention employs high-pressure homogenization technology combined with high-speed shearing process, which can further reduce the particle size of minerals in the suspension to the nanoscale. The fat-soluble vitamin emulsion droplets are also finer and more uniform, and the improved dispersion uniformity further enhances the product stability. The gritty feeling of large particles when drinking is also improved, and the palatability is significantly enhanced.

[0060] This invention has been experimentally tested. The nano-suspension prepared by high-pressure homogenization was analyzed using a laser particle size analyzer. The results showed that the particle size and dispersion uniformity of the product were significantly improved. The introduction of polysaccharide raw materials also significantly reduced the aggregation phenomenon of the suspension after high-pressure homogenization. Furthermore, by comparing changes in vitamin and mineral content, the nutrient stability of this nano-suspension was also found to be high.

[0061] To further understand the present invention, the following embodiments illustrate the vitamin and mineral-containing nano-suspension and its preparation method provided by the present invention. The scope of protection of the present invention is not limited by the following embodiments.

[0062] Comparative Example 1

[0063] The components are as follows: 15 parts calcium citrate, 6 parts magnesium gluconate, 0.5 parts zinc gluconate, 0.1 parts zinc-enriched edible yeast powder, 6 parts fructooligosaccharides, 0.035 parts vitamin D, 0.2 parts xanthan gum, 1 part gum arabic, 1 part citric acid, 10 parts xylitol, and 60 parts purified water. Accurately weigh xylitol, xanthan gum, gum arabic, and fructooligosaccharides and add them to purified water. Dissolve by stirring at 70°C and 600 rpm. After cooling to room temperature, add magnesium gluconate, zinc gluconate, citric acid, calcium citrate, and vitamin D. Shear at 5000 rpm for 5 minutes. Filter to obtain the final product.

[0064] Comparative Example 2

[0065] The components are as follows: 15 parts calcium citrate, 6 parts magnesium gluconate, 0.5 parts zinc gluconate, 0.1 parts zinc-enriched edible yeast powder, 6 parts fructooligosaccharides, 0.035 parts vitamin D, 0.2 parts xanthan gum, 1 part gum arabic, 1 part citric acid, 10 parts xylitol, and 60 parts purified water. After accurately weighing xylitol, xanthan gum, gum arabic, and fructooligosaccharides, add them to purified water and stir at 70℃ and 600 rpm to dissolve. After cooling to room temperature, grind the calcium citrate and add it to the mixture along with magnesium gluconate, zinc gluconate, citric acid, and vitamin D. Filter the sample and place it in a high-pressure homogenizer for homogenization at 30 MPa once.

[0066] Example 1

[0067] The components are as follows: 15 parts calcium citrate, 6 parts magnesium gluconate, 0.5 parts zinc gluconate, 0.1 parts zinc-enriched edible yeast powder, 6 parts fructooligosaccharides, 0.035 parts vitamin D, 0.2 parts xanthan gum, 1 part gum arabic, 1 part citric acid, 10 parts xylitol, and 60 parts purified water. After accurately weighing xylitol, xanthan gum, gum arabic, and fructooligosaccharides, add them to purified water at 70℃ and stir at 600 rpm to dissolve. After cooling to room temperature, add magnesium gluconate, zinc gluconate, citric acid, calcium citrate, and vitamin D. Shear the mixture at 5000 rpm for 5 minutes. Finally, filter the sheared sample and place it in a high-pressure homogenizer for homogenization at 40 MPa once.

[0068] Example 2

[0069] The components are as follows: 15 parts calcium citrate, 6 parts magnesium gluconate, 0.5 parts zinc gluconate, 0.1 parts zinc-enriched edible yeast powder, 6 parts fructooligosaccharides, 0.035 parts vitamin D, 0.2 parts xanthan gum, 1 part gum arabic, 1 part citric acid, 10 parts xylitol, and 60 parts purified water. After accurately weighing xylitol, xanthan gum, gum arabic, and fructooligosaccharides, add them to purified water at 70℃ and stir at 600 rpm to dissolve. After cooling to room temperature, add magnesium gluconate, zinc gluconate, citric acid, calcium citrate, and vitamin D. Shear the mixture at 5000 rpm for 5 minutes. Finally, filter the sheared sample and place it in a high-pressure homogenizer for homogenization at 70 MPa once.

[0070] Example 3

[0071] The components are as follows: 15 parts calcium citrate, 6 parts magnesium gluconate, 0.5 parts zinc gluconate, 0.1 parts zinc-enriched edible yeast powder, 6 parts fructooligosaccharides, 0.035 parts vitamin D, 0.2 parts xanthan gum, 1 part gum arabic, 1 part citric acid, 10 parts xylitol, and 60 parts purified water. After accurately weighing xylitol, xanthan gum, gum arabic, and fructooligosaccharides, add them to purified water at 70℃ and stir at 600 rpm to dissolve. After cooling to room temperature, add magnesium gluconate, zinc gluconate, citric acid, calcium citrate, and vitamin D. Shear the mixture at 5000 rpm for 5 minutes. Finally, filter the sheared sample and place it in a high-pressure homogenizer for homogenization at 70 MPa for two cycles to obtain the final product.

[0072] Example 4

[0073] The components are as follows: 15 parts calcium citrate, 6 parts magnesium gluconate, 0.5 parts zinc gluconate, 0.1 parts zinc-enriched edible yeast powder, 6 parts fructooligosaccharides, 0.035 parts vitamin D, 0.2 parts xanthan gum, 1 part gum arabic, 1 part citric acid, 10 parts xylitol, and 60 parts purified water. After accurately weighing xylitol, xanthan gum, gum arabic, and fructooligosaccharides, add them to purified water at 70℃ and stir at 600 rpm to dissolve. After cooling to room temperature, add magnesium gluconate, zinc gluconate, citric acid, calcium citrate, and vitamin D. Shear the mixture at 5000 rpm for 5 minutes. Finally, filter the sheared sample and place it in a high-pressure homogenizer, homogenizing it three times at 70 MPa.

[0074] Examples 5-14

[0075] The components are as follows: 15 parts calcium citrate, 6 parts magnesium gluconate, 0.5 parts zinc gluconate, 0.1 parts zinc-enriched edible yeast powder, 6 parts fructooligosaccharides, 0.035 parts vitamin D, 0.2 parts xanthan gum, 1 part gum arabic, 1 part citric acid, 10 parts xylitol, and 60 parts purified water. After accurately weighing xylitol, xanthan gum, gum arabic, and fructooligosaccharides, add them to purified water and stir at 70°C and 600 rpm to dissolve. After cooling to room temperature, add magnesium gluconate, zinc gluconate, citric acid, calcium citrate, and vitamin D. Shear at high speed for 5 minutes at 5000 rpm. Finally, add 0.5 parts of charge stabilizer (Tremella fuciformis polysaccharide (Example 5), yeast polysaccharide (Example 6), sodium chondroitin sulfate (Example 7), disodium hydrogen phosphate (Example 8), sodium tripolyphosphate (Example 9), sodium hexametaphosphate (Example 10), fucoidan (Example 11), citrus fiber (Example 12), sodium hyaluronate (Example 13), and chitosan (Example 14)) to the sheared sample, dissolve completely, filter, and place in a high-pressure homogenizer. Homogenize at 70 MPa for 3 cycles to obtain the final product.

[0076] Examples 15-17

[0077] The components are as follows: 15 parts calcium citrate, 6 parts magnesium gluconate, 0.5 parts zinc gluconate, 0.1 parts zinc-enriched edible yeast powder, 6 parts fructooligosaccharides, 0.035 parts vitamin D, 0.2 parts xanthan gum, 1 part gum arabic, 1 part citric acid, 10 parts xylitol, and 55 parts purified water. After accurately weighing xylitol, xanthan gum, gum arabic, and fructooligosaccharides, they were added to purified water and stirred at 70°C and 600 rpm to dissolve. After cooling to room temperature, magnesium gluconate, zinc gluconate, citric acid, calcium citrate, and vitamin D were added. The mixture was sheared at 5000 rpm for 5 minutes. Finally, 5 parts of charge stabilizer (Tremella fuciformis polysaccharide (Example 15), yeast polysaccharide (Example 16), and chondroitin sulfate sodium (Example 17)) were added to the sheared sample, fully dissolved, filtered, and then placed in a high-pressure homogenizer. The homogenization cycle was repeated three times at 70 MPa to obtain the final product.

[0078] Test case

[0079] Test items:

[0080] 1. The above embodiments and comparative examples were tested by laser particle size analyzer and observed under an optical microscope.

[0081] 2. Zeta potentials were measured for the above embodiments and comparative examples.

[0082] 3. The contents of calcium, magnesium, zinc and vitamin D nutrients in the above examples and comparative examples were tested.

[0083] Test method:

[0084] 1.1 Test substance ratio

[0085] Table 1. Proportions of the test substances

[0086]

[0087] 1.2 Observation Indicators and Measurement Methods

[0088] Average particle size: The average particle size is the sum of the products of each particle size multiplied by its percentage in the particle size distribution, and then divided by 100.

[0089] D10 and D90: These represent the particle sizes corresponding to a cumulative particle size distribution percentage of 10% and 90%, respectively, reflecting the characteristics of smaller and larger particles.

[0090] Polydispersion uniformity (PI): Calculated by the ratio of the standard deviation of particle size (SD) to the average particle size (d). The PI value ranges from 0 to 1. The smaller the value, the more concentrated the particle size distribution and the more uniform the particle size. Conversely, if the PI value is close to 1, it means that the particle size distribution is too wide and the particle size difference is large.

[0091] Zeta potential: The zeta potential is a measure of the strength of the repulsive or attractive forces between particles. The higher the absolute value (positive or negative), the more stable the system, meaning that dissolution or dispersion can resist aggregation (generally, an absolute value above 30 is considered to represent high stability). Conversely, the lower the zeta potential (positive or negative), the more inclined the system is to condense or aggregate, meaning that the attractive force exceeds the repulsive force, and dispersion is disrupted, resulting in condensation or aggregation (it should be noted that the absolute value of the zeta potential represents its stability, and positive or negative indicates the type of charge carried by the particles).

[0092] 1.3 Main Instruments

[0093] PSS Z3000 laser particle size analyzer, from Particle Sizing Systems (PSS), USA;

[0094] 1.4 Data Statistical Methods

[0095] The results were statistically analyzed using Minitab Statistical Software.

[0096] Test results:

[0097] 2.1 Comparison of particle size and dispersion uniformity results of the test samples:

[0098] Table 2 Particle size and dispersion uniformity of the test samples

[0099]

[0100] Laser particle size analyzer results show that high-pressure homogenization can reduce the particle size of vitamin and mineral suspension from the micrometer level to below the micrometer level, reaching the nanometer level; it can also reduce the dispersion uniformity value. The lower the value, the more uniform the particle size of the system, which is more conducive to the stability of the system.

[0101] The experimental results above show that the process conditions of high-pressure homogenization cycling at 70 MPa for 3 cycles can reduce the particle size to below 1 μm, achieving the nanoscale overall. With increasing cycle number, the average particle size decreases significantly, and the particle size distribution uniformity is significantly improved. Since particle size and dispersion uniformity represent the instantaneous particle size distribution in the sample suspension state and cannot reflect the charge interaction between particles, while zeta potential is a measure of the strength of the mutual repulsion or attraction between particles, zeta potential is measured.

[0102] 2.2 Study on the effect of charge stabilizers on improving coagulation phenomenon after high-pressure homogenization:

[0103] Table 3 Zeta potentials of the test substances

[0104]

[0105] Note: * Compared with Comparative Example 1, p < 0.05; # Compared with Example 4, p < 0.05.

[0106] Zeta potential measurements showed that the potential of Comparative Example 1 (without high-pressure homogenization) was higher than that of Example 4 (which underwent high-pressure homogenization). The addition of different types of charge stabilizers partially demonstrated better charge stabilization effects. Specifically, the phosphates (disodium hydrogen phosphate, sodium tripolyphosphate, and sodium hexametaphosphate) added in Examples 8, 9, and 10 had a relatively small impact on the overall charge. This is because the chelating ability of phosphate ions in solution increases with increasing chain length, and the polyanion effect strengthens with increasing phosphate content, thus gradually increasing the charge. However, since the pH of the samples in this experiment was less than 4, the acidic environment weakened the effect of phosphates to some extent, resulting in a weaker charge stabilization effect. Although Example 10 showed a significant difference compared to Example 4, the absolute potential value was still below 30 mV, indicating limited effectiveness.

[0107] Polysaccharides exhibit different charge stabilizing effects depending on their type. In Examples 5 and 6, the tremella polysaccharide and yeast polysaccharide, due to the negative charge conferred by the hydroxyl groups on their long sugar chains, not only stabilized the fine particles and droplets generated by high-pressure homogenization through steric hindrance, but also significantly increased the overall potential, reaching an absolute value higher than 30mV, indicating a relatively stable system state. Although the fucoidan added in Example 11 contains a small amount of sulfate groups and carries a negative charge, its adsorption onto the particle surface may have altered the surface charge distribution and reduced electrostatic repulsion, leading to a decrease in the zeta potential. In Example 12, the citrus fiber, whose main component cellulose is physically adsorbed onto the particle surface through hydrogen bonds and van der Waals forces, forms a coating layer on the particle surface. The charge density of this coating layer is lower than that of the original particle surface, thus affecting the zeta potential. Sodium hyaluronate added in Example 13, due to its long-chain linear macromolecular structure, has extremely strong hydration capacity and thickening effect, resulting in complete encapsulation of the particles. Under these circumstances, the measured Zeta potential remained essentially unchanged, but the system's physical stability was improved due to steric hindrance. Conversely, chondroitin sulfate added in Example 7, as a shorter-chain linear polysaccharide with a higher charge density, differed from the steric hindrance effect of sodium hyaluronate. After adsorbing onto the particle surface, it increased the overall potential value through electrostatic repulsion, becoming more stable. Chitosan added in Example 14, due to its positive charge, immediately caused flocculation and sedimentation upon addition; therefore, no further experimental operations or tests were conducted.

[0108] A comparison of the three groups (Examples 5, 6, and 7) exhibiting charge stabilizing effects revealed no significant difference between Examples 5 and 6, but both were superior to Example 7. By increasing the addition amount to 5 parts, Examples 15, 16, and 17 also demonstrated charge stabilizing effects with higher potential values. Again, there was no significant difference between Examples 15 and 16, and both were superior to Example 17. Therefore, for cost control and economic benefits, the technical solutions described in Examples 5 and 6 should be selected whenever possible.

[0109] Furthermore, after diluting Comparative Example 1 and Examples 4, 5, 6, and 7 by 100 times with deionized water, observation under a microscope at 400x magnification (objective lens × 40x, eyepiece × 10x) revealed that the suspension prepared solely by shearing in Comparative Example 1 contained a significant number of large particles, consistent with the particle size analysis results. While the sample prepared by high-pressure homogenization in Example 4 showed a significant reduction in particle size, with no large particles remaining, significant inter-particle aggregation was observed, with small particles clustering together. In Examples 5, 6, and 7, the aggregation of particles was significantly improved after the addition of charge stabilizers. (See also...) Figure 1 , Figure 1 This refers to the microscopic state of the test substance. Figure 1In the examples: a (Comparative Example 1), b (Example 4), c (Example 5), d (Example 6), e (Example 7)

[0110] Based on the above experimental results, while high-pressure homogenization reduces particle size, the introduction of charge stabilizers such as Tremella polysaccharide, yeast polysaccharide, or sodium chondroitin sulfate can improve the aggregation and sedimentation phenomenon caused by the decrease in potential to a certain extent and enhance the stability of the system.

[0111] 2.3 Nutrient content stability study:

[0112] Table 4 Nutrient attenuation of test substances

[0113]

[0114] Note: Attenuation rate = (accelerated experiment result - initial value) / initial value * 100%, negative value represents content attenuation.

[0115] Table 4 shows that the mineral content stability was high in all groups, and the accelerated 2-month degradation rate was low. Compared to the vitamin D degradation in Comparative Example 1, the vitamin D content degradation in Examples 4, 5, 6, 7, 15, and 16 was even lower, with an accelerated 2-month degradation rate of less than 10%, while the vitamin D degradation in Comparative Example 1 was nearly 30%. This data indicates that the vitamin D content in the nano-suspension remained relatively stable. The reason for this may be that high-pressure homogenization resulted in more thorough emulsification of vitamin D oils, further reducing the droplet size and making the distribution more uniform, thus improving the stability of the product during storage. In contrast, the comparative example only used a shearing process, which resulted in insufficient emulsification, leading to demulsification and oil floating during storage, ultimately causing vitamin D to degrade more easily and its stability to be lower than expected.

[0116] In summary, high-pressure homogenization can significantly reduce the particle size of suspensions to below 1 μm, reaching the nanoscale, and greatly improve dispersion uniformity. By introducing polysaccharide raw materials, the potential value is restored to a stable state, thus solving the risk of particle aggregation and sedimentation during storage caused by high-pressure homogenization and improving product stability. Furthermore, vitamins in nano-suspensions exhibit higher stability.

[0117] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A vitamin and mineral nanosuspension, characterized in that, Based on 100 parts by weight of nano-suspension, it is obtained from the following raw materials through high-speed shearing and high-pressure homogenization: 10-50 parts nutrient supplement, 0.1-2 parts thickener, 1-6 parts emulsifier, 0.1-5 parts charge stabilizer, and the remainder is water; The charge stabilizer includes at least one of Tremella polysaccharide, yeast polysaccharide, and sodium chondroitin sulfate.

2. The vitamin and mineral nanosuspension according to claim 1, characterized in that, The nutrient supplement includes one or more of the following: vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, vitamin K, calcium salts, magnesium salts, zinc salts, iron salts, and selenium-enriched yeast.

3. The vitamin and mineral nanosuspension according to claim 1, characterized in that, The thickener includes at least one of xanthan gum, carrageenan, pectin, guar gum, locust bean gum, and sodium carboxymethyl cellulose.

4. The vitamin and mineral nanosuspension according to claim 1, characterized in that, The emulsifier includes at least one of gum arabic, mono- and diglycerides of fatty acids, phospholipids, monoglycerides of succinate, and sucrose fatty acid esters.

5. The vitamin and mineral nano-suspension according to claim 1, characterized in that, It also includes acidity regulators; The acidity regulator includes at least one of citric acid, DL-malic acid, fumaric acid, sodium citrate, potassium citrate, lactic acid, and sodium bicarbonate. Based on 100 parts by weight of nano suspension, it includes 0.1-3 parts of acidity regulator.

6. The vitamin and mineral nanosuspension according to claim 1, characterized in that, It also includes sweeteners; The sweetener is selected from at least one of sorbitol, sorbitol liquid, xylitol, erythritol, maltitol, maltitol liquid, isomaltitol, isomaltulose, mannitol, sucrose, trehalose, glucose, mogrosides, sucralose, and steviol glycosides. Based on 100 parts by weight of nano suspension, it includes 5-20 parts of sweetener.

7. The vitamin and mineral nanosuspension according to claim 1, characterized in that, It also includes prebiotics; The prebiotic is selected from at least one of fructooligosaccharides, galactooligosaccharides, inulin, resistant dextrin, polydextrose, and isomaltooligosaccharides; Based on 100 parts by weight of nano-suspension, it includes 2-20 parts of prebiotics.

8. The vitamin and mineral nanosuspension according to claim 1, characterized in that, It also includes other synergistic ingredients, which are selected from at least one of yeast β-glucan, glucosamine, zinc-enriched edible yeast powder, yeast extract, and glutathione yeast powder; Based on 100 parts by weight of nano-suspension, it includes 0.1-10 parts of other synergistic ingredients.

9. A method for preparing a vitamin and mineral nanosuspension as described in any one of claims 1 to 8, characterized in that, Includes the following steps: A) Mix the thickener, emulsifier, and water, heat and stir to obtain a mixed solution; B) After cooling the mixed solution, add the nutrient supplement and perform high-speed shearing, then add the charge stabilizer, mix and stir, and filter to obtain the mixture. C) The mixture is homogenized under high pressure, then filled and sterilized to obtain a vitamin and mineral nano suspension.

10. The preparation method according to claim 9, characterized in that, In step A), the stirring speed is 500-1000 rpm; In step B), the high-speed shearing speed is 2000-9000 rpm, and the time is 5-20 min; In step C), the pressure of the high-pressure homogenization is 40~70MPa, the number of cycles is 2-5, and the temperature during the high-pressure homogenization process is always controlled to be below 40℃.

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