Sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition and preparation method thereof

Abstract

The invention discloses a sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition and a preparation method thereof, the sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition is of a core-shell double-phase structure, and the sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition comprises an aqueous phase core, an aqueous phase core and a composite material, the active ingredient of the aqueous phase core is a covalent bond of carboxymethylated recombinant human collagen III and eclipta oligopeptide; the oil-phase layer wraps the water-phase core and comprises a sebum-imitating three-phase film as well as an antioxidant active matter and a slow-release carrier which are dispersed in the sebum-imitating three-phase film; wherein the volume ratio of the water-phase core to the oil-phase layer is 1: 2-1: 4, a core-shell double-phase structure with the carboxymethylated recombinant collagen-eclipta oligopeptide covalent bond as the water-phase core and the sebum-imitating three-phase membrane functional carrier as the oil-phase layer is constructed, and the limitation that active ingredients in a traditional formula are simply mixed is broken through. According to the structure, the stability of the water phase core is ensured through a chemical covalent bond, and the percutaneous permeability and biocompatibility of the composition are remarkably improved by utilizing the high similarity of a sebum-imitating film and skin lipid.

Description

Technical Field

[0001] This invention belongs to the field of cosmetics and skin care products technology, and particularly relates to a sebum-inspired recombinant collagen antioxidant, anti-aging, and brightening composition and its preparation method. Background Technology

[0002] With the increasing demand for anti-aging and brightening skin care, the application of bioactive ingredients in cosmetics and skincare products has become a research focus. Recombinant human collagen, due to its excellent biocompatibility, low immunogenicity, and well-defined repair functions, is considered an ideal alternative to traditional animal-derived collagen. It is often compounded with various plant extracts (such as polyphenols and flavonoids) to achieve multiple effects such as anti-oxidation and promoting collagen regeneration. To improve the stability and transdermal efficiency of active ingredients, delivery system technologies are constantly evolving, such as using carriers like liposomes and polymer microspheres for encapsulation. Core-shell structures have attracted attention due to their ability to achieve physical isolation and controlled release of contents. Simultaneously, biomimetic liposomes that mimic the natural lipid composition of the skin (such as systems containing ceramides and sterols) have also been developed to improve the skin affinity and barrier repair function of formulations. Existing technologies utilize various physical mixing, encapsulation, or simple chemical modification methods to synergistically address complex issues such as skin aging, pigmentation, and oxidative stress.

[0003] However, existing technologies still have systemic shortcomings. Most compositions merely physically mix or simply encapsulate recombinant collagen with plant active ingredients, lacking stable chemical bonds. This leads to the active components easily dissociating and becoming inactive during storage or delivery, and makes it difficult to achieve synergistic effects. Common delivery carriers (such as ordinary liposomes or emulsions) have weak responsiveness to the skin microenvironment, failing to achieve intelligent sustained release of active ingredients at the site of action, often resulting in rapid release but short duration of action. Furthermore, traditional biomimetic lipid systems mostly focus on moisturizing and barrier repair, with insufficient functional integration with core anti-aging active ingredients, making it difficult to simultaneously achieve a comprehensive effect of long-lasting anti-oxidation, deep collagen promotion, and immediate brightening. At the process level, existing methods for modifying collagen are often random and uncontrollable, making it difficult to precisely introduce specific functional groups to optimize its interaction with other ingredients and the stability of the final formulation. Therefore, developing an integrated core-shell structure system that can achieve stable covalent binding of active ingredients, has environmentally responsive sustained-release capabilities, and exhibits high synergy between the biomimetic carrier and the active ingredients has become a key challenge for improving the overall performance of products. Summary of the Invention

[0004] In order to overcome the above-mentioned defects of the prior art, the present invention provides a sebum-inspired recombinant collagen antioxidant, anti-aging and brightening composition and preparation method, which solves the problems in the prior art of achieving stable covalent bonding of active ingredients, intelligent sustained release and functional synergy with biomimetic carriers.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A sebum-inspired recombinant collagen antioxidant, anti-aging, and brightening composition and its preparation method, exhibiting a core-shell biphasic structure, comprising: The aqueous core contains a covalently bonded compound of carboxymethylated recombinant human type III collagen and Eclipta prostrata oligopeptide. The oil phase layer encapsulates the aqueous core, containing a sebum-mimicking triphase membrane and dispersed antioxidants and sustained-release carriers therein; The volume ratio of the aqueous phase core to the oil phase layer is 1:2 to 1:4.

[0006] Preferably, the degree of modification of carboxymethylated recombinant human type III collagen is 0.8 to 1.2 mmol / g, and the mass ratio of carboxymethylated recombinant human type III collagen to Eclipta prostrata oligopeptide in the covalent compound is 5:1 to 10:1.

[0007] Preferably, the pseudo-sebum triphase membrane is composed of soybean sterol, linoleic acid and ceramide 3B in a weight ratio of 3-4:2-3:1.

[0008] Preferably, the antioxidant active ingredients include winged fruit oil tree seed polyphenols and sea buckthorn seed shell flavonoids, and the sustained-release carrier includes poly-β-amino esters; based on the total weight of the oil phase layer, the content of winged fruit oil tree seed polyphenols is 0.5% to 2.0%, the content of sea buckthorn seed shell flavonoids is 0.05% to 0.1%, and the content of poly-β-amino esters is 0.1% to 0.3%.

[0009] Preferably, the oil phase layer further contains magnesium aluminum silicate, the content of which is 0.2% to 0.4% of the total weight of the oil phase layer; and the polyβ-amino ester degrades in an environment of pH 4.5-5.5, so that the composition has the active ingredient sustained-release characteristics for 8-12 hours.

[0010] Preferably, it also includes a moisturizing agent, which is composed of sodium hyaluronate and panthenol in a mass ratio of 1-2:1.

[0011] Preferably, a method for preparing a sebum-inspired recombinant collagen antioxidant, anti-aging, and brightening composition includes the following steps: (1) Preparation of aqueous core: Recombinant human type III collagen was modified by carboxymethylation and then covalently linked with Eclipta prostrata oligopeptide to obtain aqueous core; (2) Preparation of oil phase layer: The components of the imitation sebum three-phase membrane are melt-mixed, and then antioxidant actives and sustained-release carriers are added and dispersed evenly to obtain the oil phase layer; (3) Formation of core-shell structure: Under stirring conditions, the aqueous core is added to the oil phase layer to form a core-shell biphase structure composition.

[0012] Preferably, in step (1), the carboxymethylation modification is carried out by reacting with sodium chloroacetate in a phosphate buffer system at pH 7.2 to 7.5 and temperature 35 to 40°C for 4 to 6 hours under the catalysis of papain, and the amount of papain used is 0.5% to 1.0% of the weight of recombinant human type III collagen.

[0013] Preferably, in step (1), the covalent bonding reaction is carried out at pH 6.8 to 7.0 and temperature 25 to 30°C for 2 to 3 hours.

[0014] Preferably, in step (3), the stirring conditions are a temperature of 35 to 40°C, a rotation speed of 500 to 800 r / min, and a stirring time of 30 to 40 minutes.

[0015] The technical effects and advantages of the present invention, which describes a sebum-inspired recombinant collagen antioxidant, anti-aging, and brightening composition and its preparation method, are as follows: 1. This invention overcomes the limitations of simple mixing of active ingredients in traditional formulations by constructing a core-shell biphase structure with a "carboxymethylated recombinant collagen-Eclipta prostrata oligopeptide covalently bonded compound" as the aqueous core and a "sebum-mimicking triphase membrane functional carrier" as the oil phase. This structure not only ensures the stability of the aqueous core through chemical covalent bonds, but also significantly improves the transdermal permeability and biocompatibility of the composition by utilizing the high similarity between the sebum-mimicking membrane (soybean sterol / linoleic acid / ceramide 3B) and skin lipids. Specific antioxidants dispersed in the oil phase (Pterocarpus santalinus seed polyphenols, Hippophae rhamnoides seed shell flavonoids) and the pH-responsive sustained-release carrier (poly-β-amino ester) work synergistically to achieve efficient inhibition of multiple targets such as free radicals and tyrosinase, while ensuring the continuous and controllable release of active ingredients in a weakly acidic skin environment (up to 8-12 hours), thus achieving a unified effect of immediate and long-lasting anti-oxidation, anti-aging, and skin brightening efficacy.

[0016] 2. The unique core-shell structure and the addition of components such as magnesium aluminum silicate endow the composition with excellent physical and chemical stability, effectively preventing common problems such as delamination and inactivation during storage. The biomimetic sebum membrane system itself has the function of simulating and supplementing intercellular lipids. In synergy with optional moisturizing aids (sodium hyaluronate / panthenol), it can further strengthen the skin's moisturizing barrier function, promoting skin health while exerting the core effects of anti-aging and brightening. In addition, the preparation method protected by the claims (such as papain-directed carboxymethylation, core-shell formation under specific temperature and stirring conditions, etc.) has clear, controllable, and reproducible process conditions, providing a reliable path for large-scale production and ensuring the consistency and effectiveness of the final product performance. Attached Figure Description

[0017] Figure 1 This is a flowchart illustrating the preparation process of a sebum-inspired recombinant collagen antioxidant, anti-aging, and brightening composition and its preparation method, as proposed in this invention. Detailed Implementation

[0018] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0019] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include," "contain," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "includes..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0020] refer to Figure 1 This invention provides a sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition and its preparation method. The composition has a core-shell biphase structure. Its aqueous core is a covalently bonded compound of carboxymethylated recombinant human type III collagen and Eclipta prostrata oligopeptide. The oil phase layer contains a sebum-mimicking triphase membrane and antioxidant actives and sustained-release carriers dispersed therein. The volume ratio of the aqueous core to the oil phase layer is 1:2 to 1:4. The composition is prepared by the following method: (1) Preparation of the aqueous core: Carboxymethylation modification of recombinant human type III collagen, followed by covalent linkage with Eclipta prostrata oligopeptide; (2) Preparation of the oil phase layer: Melting and mixing the sebum-mimicking triphase membrane components, adding antioxidant actives and sustained-release carriers and dispersing them evenly; (3) Formation of the core-shell structure: Adding the aqueous core into the oil phase layer under stirring conditions to form a core-shell structure. This invention achieves stable binding, long-lasting sustained release, and synergistic effects of active ingredients through chemical modification, covalent bonding, and biomimetic membrane encapsulation technology. The product has excellent antioxidant, anti-aging, skin brightening, and skin barrier repair functions, and the process is controllable and has good stability.

[0021] Example 1 Standard process and formulation composition: Purpose of implementation: This invention demonstrates the complete process and comprehensive effects of preparing a composition with a core-shell dual-phase structure and possessing highly efficient antioxidant, anti-aging, brightening, and sustained-release functions under the preferred process parameters and component ratios of this invention.

[0022] Ingredients and dosage: Aqueous core raw materials: 10.0 g recombinant human type III collagen, 7.5 g sodium chloroacetate, 0.08 g papain, and 0.25 g Eclipta prostrata oligopeptide.

[0023] Oil phase raw materials: 4.0 g soybean sterol, 3.0 g linoleic acid, 1.0 g ceramide 3B, 0.1 g winged fruit oil tree seed polyphenols, 0.01 g sea buckthorn seed shell flavonoids, 0.02 g poly-β-amino ester, 0.03 g magnesium aluminum silicate.

[0024] Other: 500 mL of phosphate-buffered saline (PBS, 0.1 M, pH 7.3) and 200 mL of PBS at pH 6.9.

[0025] Implementation steps: (1) Preparation of aqueous core: a. Carboxymethylation modification: 10.0 g of recombinant human type III collagen was dissolved in 500 mL of phosphate buffer (pH 7.3). 7.5 g of sodium chloroacetate and 0.08 g of papain were added, and the mixture was placed in a constant-temperature reactor at 37 °C and gently stirred for 5 hours. After the reaction, the mixture was transferred to a dialysis bag with a molecular weight cutoff of 10 kDa and purified by dialyzing with deionized water for 48 hours. The purified solid was then freeze-dried to obtain a white flocculent solid. The degree of carboxymethylation modification was determined to be 1.05 mmol / g collagen.

[0026] b. Covalent bonding: 2.0 g of the modified collagen solid was dissolved in 200 mL of phosphate buffer (pH 6.9). 0.25 g of high-purity Eclipta prostrata oligopeptide was added, and the mixture was stirred continuously at 28 °C for 2.5 hours. The reaction solution was concentrated by ultrafiltration (using a 10 kDa membrane) and then freeze-dried to obtain the aqueous core active complex.

[0027] (2) Preparation of the oil phase layer: a. Place 4.0 g of soybean sterol, 3.0 g of linoleic acid and 1.0 g of ceramide 3B (purity ≥96%) in a container and heat in a 70°C water bath to melt them, forming a homogeneous and transparent imitation sebum base solution.

[0028] b. Maintaining the system temperature at 60°C, while stirring at a constant speed, add 0.1 g of *Hippophae rhamnoides* seed polyphenols, 0.01 g of *Hippophae rhamnoides* seed flavonoids, 0.02 g of poly-β-amino ester, and 0.03 g of magnesium aluminum silicate to the base solution sequentially. Continue stirring until all additives are uniformly dispersed to obtain a clear oil phase system, then cool to 38°C for later use.

[0029] (3) Construction of core-shell dual-phase structure: 1.0 g of the aqueous core-shell composite obtained in step (1) was dispersed in 3.0 mL of deionized water to form a homogeneous aqueous phase. Under stirring conditions of 38 °C and 600 rpm, this aqueous phase was slowly added dropwise to 20 mL of the oil phase layer prepared in step (2) using a constant flow pump. After the addition was complete, stirring was continued for 35 minutes under the same conditions. Heating was then stopped, and the system was allowed to cool naturally to room temperature, ultimately yielding a milky white, homogeneous core-shell biphase structure composition.

[0030] Implementation results: The prepared composition was tested and found to have a DPPH free radical scavenging rate of 92.5%, demonstrating strong in vitro antioxidant capacity; its tyrosinase inhibition rate was 68.3%, indicating significant brightening potential. In a release medium simulating the slightly acidic environment of skin (pH 5.0), the time required for 80% cumulative release of the active ingredients was 10.5 hours, exhibiting excellent sustained-release properties. In a human dermal fibroblast oxidative damage model, the composition increased cell survival rate by 85.3% and promoted a 55.6% increase in collagen I secretion. After 30 days of accelerated stability testing at 40°C, the product remained stable, with the main active ingredients retaining over 95% of their original value.

[0031] Example 2 Key parameter lower limit composition: Purpose of implementation: The study verified that the composition could still achieve the expected basic function when the key process parameters and component contents were at the lower limit of the range described in this invention.

[0032] Adjustments to ingredients and dosages will be implemented. The amount of papain used was adjusted to 0.5% of the weight of recombinant human type III collagen, and the modification reaction time was shortened to 4 hours.

[0033] The mass ratio of carboxymethylated collagen to Eclipta prostrata oligopeptide was 5:1, and the covalent reaction time was 2 hours.

[0034] In the oil phase, the amounts of winged tamarisk seed polyphenols, sea buckthorn seed shell flavonoids, poly-β-amino esters, and magnesium aluminum silicate were adjusted to 0.5%, 0.05%, 0.1%, and 0.2% of the total weight of the oil phase, respectively.

[0035] When constructing the core-shell structure, the volume ratio of the aqueous phase to the oil phase was adjusted to 1:4.

[0036] Implementation steps: The basic steps are the same as in Example 1, except that the parameters were adjusted accordingly. The final carboxymethylated collagen modification degree was 0.82 mmol / g.

[0037] Implementation results: The resulting composition exhibited a DPPH radical scavenging rate of 88.3%, a tyrosinase inhibition rate of 62.1%, and a sustained-release time (cumulative release of 80%) of 8.8 hours. In cell experiments, the protective effect against oxidative damage (80.1% increase in cell survival) and the collagen secretion-promoting effect (48.9% increase) remained evident. These results demonstrate that even using the lower limit of the range parameters, the composition of this invention maintains significantly superior overall performance compared to conventional products.

[0038] Example 3 Key parameter upper limit composition: Purpose of implementation: Verify the performance and stability of the composition when the key process parameters and component contents are at the upper limit of the range described in this invention.

[0039] Adjustments to ingredients and dosages will be implemented. The amount of papain used was increased to 1.0% of the weight of recombinant human type III collagen, and the modification reaction time was extended to 6 hours.

[0040] The mass ratio of carboxymethylated collagen to Eclipta prostrata oligopeptide was 10:1, and the covalent reaction time was 3 hours.

[0041] In the oil phase, the amount of each functional component added was increased to the upper limit of the total weight of the oil phase: 2.0% of winged fruit oil tree seed polyphenols, 0.1% of sea buckthorn seed shell flavonoids, 0.3% of poly-β-amino esters, and 0.4% of magnesium aluminum silicate.

[0042] When constructing the core-shell structure, the volume ratio of the aqueous phase to the oil phase was adjusted to 1:2.

[0043] Implementation steps: The basic steps are the same as in Example 1, performed according to the above-mentioned upper limit parameters. The final carboxymethylated collagen modification degree is 1.18 mmol / g.

[0044] Implementation results: The resulting composition exhibits robust performance: a DPPH free radical scavenging rate of 90.7% and a tyrosinase inhibition rate of 65.5%. Due to the high poly-β-amino ester content, the sustained-release period is extended to 11.8 hours. Cell experiments showed that it increased cell viability by 83.5% and promoted a 52.3% increase in collagen I secretion. Accelerated stability testing demonstrated product stability.

[0045] Example 4 Imitation sebum film ratio adjustment composition: Purpose of implementation: The effect of varying the proportions of each component in the simulated sebum three-phase membrane on the final properties of the composition was investigated.

[0046] Adjustments to ingredients and dosages will be implemented. The weight ratio of soybean sterol, linoleic acid, and ceramide 3B in the simulated sebum three-phase membrane was adjusted to 3:2:1, while the dosage of other components and preparation steps were exactly the same as in Example 1.

[0047] Implementation results: The composition prepared using the adjusted ratio exhibited a DPPH free radical scavenging rate of 91.0%, a tyrosinase inhibition rate of 66.8%, a sustained-release time of 9.8 hours, and cell protection and collagen production promotion effects of 83.0% and 51.8%, respectively. These results demonstrate that adjusting the composition of the biomimetic sebum membrane within this ratio range still yields a composition with excellent performance, proving the rationality and feasibility of this ratio range.

[0048] Example 5 Complex moisturizing functional composition: Purpose of implementation: Based on the core-shell biphasic composition of this invention, moisturizing aids are added to achieve enhanced skin barrier repair and moisturizing effects.

[0049] Adjustments to ingredients and dosages will be implemented. Based on the composition obtained in Example 1, an additional moisturizing agent was added. The moisturizing agent consisted of sodium hyaluronate and panthenol in a mass ratio of 2:1, and its total addition amount accounted for 0.5% of the total weight of the final composition.

[0050] Implementation steps: After preparing the core-shell biphase composition according to the steps in Example 1 and cooling it to room temperature, slowly add the pre-prepared aqueous solution of the moisturizing agent while stirring gently until it is homogeneous.

[0051] Implementation results: This composition not only retains all the excellent properties of Example 1 (such as antioxidant, sustained release, and cell repair promotion), but its function of strengthening the skin barrier is particularly outstanding. In 3D skin model testing, after using this composition for 24 hours, the transepidermal water loss reduction rate reached 45.5%, which is significantly higher than that of Example 1 without added moisturizing agents (38.2%), demonstrating the synergistic effect brought about by the additional technical features.

[0052] Comparative Example 1 Traditional simple physical mixing emulsions: Purpose of implementation: By comparing this invention with traditional physical mixing methods that are completely different from the structural and chemical modifications of this invention, the technical advantages brought about by the core-shell structure, chemical modification, and system synergy of this invention are highlighted.

[0053] Implementation ingredients and preparation: The same amount of each raw material component as in Example 1 was used, but the preparation method was completely different: a. Without carboxymethylation modification of collagen, ordinary recombinant human type III collagen and Eclipta prostrata oligopeptide are simply mixed and dispersed in the aqueous phase.

[0054] b. The oil phase components (soybean sterols, linoleic acid, ceramide 3B and all functional additives) are simply mixed after being heated and melted.

[0055] c. Emulsify the above aqueous phase and oil phase directly at room temperature using a high-speed homogenizer (10,000 rpm) for 5 minutes to prepare a common O / W type emulsion.

[0056] Implementation results: The comparative product performed significantly worse in multiple tests: its DPPH free radical scavenging rate was only 75.4%, and its tyrosinase inhibition rate was only 45.2%. In the release experiment, the active ingredient was released rapidly in the initial stage (significant burst release effect), with more than 80% released within 2.5 hours, failing to achieve long-term sustained release. Cell experiments showed limited effects in cell protection (62.7% increase in survival rate) and collagen production promotion (25.4% increase). Most importantly, the product exhibited poor physical stability, showing significant oil-water separation after 30 days of accelerated testing at 40°C, with the active ingredient retention rate below 80%. This comparative example fully demonstrates that without the core structural design and chemical modification of this invention, simply stacking components cannot achieve the high efficiency, long-term effectiveness, and stable comprehensive technical effects achieved by this invention.

[0057] Compared with Examples 1-5 and Comparative Example 1, through a systematic comparison of Examples 1-5 and Comparative Example 1, the technical solution provided by the present invention has a revolutionary advantage over the traditional simple mixing process in terms of core efficacy, mechanism of action and product stability. Its fundamental advantage lies in a series of innovative designs of structured and chemical modifications.

[0058] First, in terms of core efficacy and mechanism of action, the composition of this invention achieves a qualitative leap from "component stacking" to "system integration." Examples 1 to 5, through their precise "core-shell structure" formed by the aqueous core composed of carboxymethylated collagen-oligopeptide covalent bonds and the oil phase layer encapsulated by a sebum-mimicking three-phase film, synergistically exert effects far exceeding those of the simple physically mixed emulsion in Comparative Example 1. Specifically, in terms of antioxidant properties, the DPPH free radical scavenging rate of the examples (88.3%-92.5%) is significantly higher than that of the comparative example (75.4%); in terms of brightening potential, the inhibition rate of tyrosinase in the examples (62.1%-68.3%) far exceeds that of the comparative example (45.2%). Crucially, the unique structure of this invention enables intelligent sustained release; in the examples, 80% of the active ingredient is released within 8.8-11.8 hours, while the comparative example experiences rapid burst release within 2.5 hours, failing to achieve a long-lasting effect. At the cellular level, the demonstrated efficacy of the embodiment in protecting oxidatively damaged cells (increasing survival rate by 80.1%-85.3%) and promoting collagen regeneration (increasing secretion by 48.9%-55.6%) completely outperformed the comparative example (62.7% and 25.4%, respectively). The mediocre data of Comparative Example 1 prove that without targeted chemical modification, covalent bonding, and biomimetic membrane encapsulation, simply relying on the crude mixing of the same components cannot activate the synergistic effects between the components, resulting in low and short-lived efficacy.

[0059] Secondly, this invention also demonstrates superior characteristics in product stability, functional scalability, and robustness of the technical solution. All products in the embodiments maintained stable properties and an activity retention rate exceeding 92% after 30 days of accelerated testing at 40°C, while Comparative Example 1 showed severe oil-water separation and a loss of activity exceeding 20%, revealing the decisive role of the core-shell structure in the physical stability of the system. Furthermore, Example 5, through simple compounding of moisturizing agents, further enhanced the barrier repair function (reduction of transepidermal water loss to 45.5%) on top of its already excellent efficacy, demonstrating the good functional scalability of the invention platform. More importantly, Examples 2 to 4 show that even with moderate adjustments to the process parameters and component ratios protected by the claims of this invention, the overall performance of the resulting products remains high and significantly better than the comparative examples. This fully demonstrates the tolerance and feasibility of the technical solution of this invention, rather than an isolated "point" invention. In conclusion, the complete defeat of Comparative Example 1 powerfully demonstrates from the opposite perspective that the inventive core of this invention—the "core-shell biphase structure," "chemically modified covalent bonding," and "sebum-like synergistic system"—are indispensable and together constitute an efficient, long-lasting, stable, and flexibly controllable advanced delivery and efficacy system.

[0060] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of protection of the claims.

[0061] In conclusion, the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition, characterized in that, It exhibits a core-shell dual-phase structure, including: The aqueous core contains a covalently bonded compound of carboxymethylated recombinant human type III collagen and Eclipta prostrata oligopeptide. The oil phase layer encapsulates the aqueous core, containing a sebum-mimicking triphase membrane and dispersed antioxidants and sustained-release carriers therein; The volume ratio of the aqueous phase core to the oil phase layer is 1:2 to 1:

4.

2. The sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition as described in claim 1, characterized in that, The degree of modification of carboxymethylated recombinant human type III collagen was 0.8 to 1.2 mmol / g, and the mass ratio of carboxymethylated recombinant human type III collagen to Eclipta prostrata oligopeptide in the covalent compound was 5:1 to 10:

1.

3. The sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition as described in claim 1, characterized in that, The pseudo-sebum triphase membrane is composed of soybean sterol, linoleic acid and ceramide 3B in a weight ratio of 3-4:2-3:

1.

4. The sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition as described in claim 1, characterized in that, The antioxidant active ingredients include winged fruit oil tree seed polyphenols and sea buckthorn seed shell flavonoids, and the sustained-release carrier includes poly-β-amino esters; based on the total weight of the oil phase, the content of winged fruit oil tree seed polyphenols is 0.5% to 2.0%, the content of sea buckthorn seed shell flavonoids is 0.05% to 0.1%, and the content of poly-β-amino esters is 0.1% to 0.3%.

5. The sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition as described in claim 4, characterized in that, The oil phase also contains magnesium aluminum silicate, which accounts for 0.2% to 0.4% of the total weight of the oil phase; and the poly-β-amino ester degrades in an environment of pH 4.5-5.5, giving the composition a sustained-release characteristic of active ingredients for 8-12 hours.

6. The sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition as described in claim 1, characterized in that, It also contains a moisturizing agent, which is composed of sodium hyaluronate and panthenol in a mass ratio of 1-2:

1.

7. A method for preparing a sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition as described in any one of claims 1 to 6, characterized in that, Includes the following steps: (1) Preparation of aqueous core: Recombinant human type III collagen was modified by carboxymethylation and then covalently linked with Eclipta prostrata oligopeptide to obtain aqueous core; (2) Preparation of oil phase layer: The components of the imitation sebum three-phase membrane are melt-mixed, and then antioxidant actives and sustained-release carriers are added and dispersed evenly to obtain the oil phase layer; (3) Formation of core-shell structure: Under stirring conditions, the aqueous core is added to the oil phase layer to form a core-shell biphase structure composition.

8. The method for preparing a sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition as described in claim 7, characterized in that, In step (1), the carboxymethylation modification is carried out by reacting with sodium chloroacetate in a phosphate buffer system at pH 7.2 to 7.5 and temperature 35 to 40°C for 4 to 6 hours under the catalysis of papain. The amount of papain used is 0.5% to 1.0% of the weight of recombinant human type III collagen.

9. The method for preparing a sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition as described in claim 7, characterized in that, In step (1), the covalent bonding reaction is carried out at pH 6.8 to 7.0 and temperature 25 to 30°C for 2 to 3 hours.

10. The method for preparing a sebum-mimicking recombinant collagen antioxidant, anti-aging, and brightening composition as described in claim 7, characterized in that, In step (3), the stirring conditions are a temperature of 35 to 40°C, a speed of 500 to 800 r / min, and a stirring time of 30 to 40 minutes.

Images