Composition with thermal protection effect as well as preparation method and application thereof

Abstract

The invention relates to the technical field of functional raw materials of cosmetics, in particular to a composition with a thermal protection effect and a preparation method and application thereof. On one hand, the composition with the thermal protection effect is provided, the cactus polysaccharide, the glucan, the Ectoin and the carnosine are combined according to a specific proportion, skin temperature rise and skin sensitivity and redness caused by thermal damage can be effectively improved, and the good thermal protection effect is achieved. The preparation method of the composition is simple in process and suitable for industrial production, and has a wide application prospect in cosmetics and / or pharmaceuticals with thermal aging resistance requirements.

Description

Technical Field

[0001] This invention relates to the field of cosmetic efficacy ingredients technology, and in particular to a composition with heat protection function, its preparation method and application. Background Technology

[0002] With increasing exposure to high-temperature environments, the demand for heat-protective ingredients in the cosmetics industry is growing. Heat can activate TRPV1 channels on cell membranes, triggering Ca²⁺ influx, activating the protein kinase C (PKC) pathway, and simultaneously inducing ROS generation. Both of these factors jointly activate the MAPK family and further upregulate MMP expression, degrading collagen and elastin in the skin, ultimately leading to premature skin aging. Furthermore, heat damage can increase the expression of angiogenesis factor VEGF and decrease the expression of anti-angiogenesis factors, causing skin redness and inflammation.

[0003] Current research on skin heat protection primarily focuses on single active ingredients or simple compound systems, such as using polysaccharides, amino acids, or small molecule protectants alone. While these methods can alleviate localized heat damage symptoms to some extent, their overall efficacy is significantly limited. Some compound systems exhibit weak heat damage mitigation effects in practical applications due to interactions between components, potentially requiring additional modification of the relevant ingredients and failing to meet the actual heat protection needs of cosmetics. Furthermore, commercially available polysaccharide raw materials often suffer from poor stability, incompatible compatibility with cosmetic systems, and insufficient efficacy, further restricting the industrial application of heat protection ingredients.

[0004] Therefore, developing a multi-component synergistic composition that can improve skin problems caused by heat damage in multiple ways, and meet the practical application needs of cosmetics for anti-heat aging and soothing heat stimulation, has become a research focus and development direction in this field.

[0005] In view of this, the present invention is hereby proposed. Summary of the Invention

[0006] One object of the present invention is to provide a composition with thermal protection effect, a method for preparing the composition and its application, which has excellent thermal protection effect.

[0007] Another object of the present invention is to provide a method for preparing the composition with the above-mentioned thermal protection effect, which is simple in process and suitable for industrial production.

[0008] A third objective of this invention is to provide an application of the composition with the above-mentioned heat protection effect in the preparation of cosmetics and / or pharmaceuticals, which has excellent heat protection effect and has broad application prospects in cosmetics and / or pharmaceuticals with anti-heat aging requirements.

[0009] To achieve the above-mentioned objectives of the present invention, one aspect of the present invention provides a composition with thermal protection effect, comprising, by weight parts, the following components:

[0010] 50-70 parts cactus polysaccharide, 25-45 parts glucan, 1-4 parts ectoine, and 1 part carnosine.

[0011] The composition with heat protection efficacy includes a specific ratio of cactus polysaccharide, glucan, ectoine, and carnosine, which can effectively improve skin temperature rise and skin sensitivity and redness caused by heat damage, and has a good heat protection effect.

[0012] Another aspect of the present invention provides a method for preparing a composition with heat protection effect, comprising: mixing cactus polysaccharide, dextran, ectoine and carnosine evenly. This method is simple and suitable for industrial production.

[0013] Another aspect of the present invention provides the use of the composition with the above-mentioned heat protection effect in the preparation of cosmetics and / or pharmaceuticals.

[0014] The above-mentioned oily skin care composition has excellent heat protection effect and has broad application prospects in cosmetics and / or pharmaceuticals with anti-heat aging requirements.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0016] This invention utilizes a specific ratio of cactus polysaccharides, dextran, ectoine, and carnosine to effectively improve skin temperature rise and skin sensitivity and redness caused by heat damage, demonstrating excellent heat protection. The preparation method of the above composition is simple and suitable for industrial production, showing broad application prospects in cosmetics and / or pharmaceuticals requiring anti-heat aging properties. Attached Figure Description

[0017] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0018] Figure 1 The figures show the composition demonstrating thermal protective efficacy in a specific embodiment of the present invention and the test results of comparative inhibition of MMP-1 expression.

[0019] Figure 2 The figures show the composition demonstrating thermal protective efficacy in a specific embodiment of the present invention and the test results of inhibiting TRPV1 expression in a comparative example.

[0020] Figure 3 This is a graph showing the skin temperature change results of a human thermal protection test on the composition demonstrating thermal protection efficacy in a specific embodiment of the present invention.

[0021] Figure 4 This is a graph showing the changes in skin sensitivity during a human thermal protection test of the composition demonstrating thermal protection efficacy in a specific embodiment of the present invention. Detailed Implementation

[0022] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings and specific embodiments. However, those skilled in the art will understand that the embodiments described below are some embodiments of the present invention, but not all embodiments, and are only used to illustrate the present invention, and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0023] The present invention provides a composition with heat protection effect, comprising, by weight parts:

[0024] 50-70 parts cactus polysaccharide, 25-45 parts oat β-glucan, 1-4 parts ectoine, and 1 part carnosine.

[0025] The composition with heat protection efficacy includes a specific ratio of cactus polysaccharide, oat β-glucan, ectoine, and carnosine, which can effectively improve skin temperature rise and skin sensitivity and redness caused by heat damage, and has a good heat protection effect.

[0026] In one specific embodiment of the present invention, the cactus polysaccharide can be commercially available cactus polysaccharide, and can be prepared using various processes including water extraction and enzymatic hydrolysis. Preferably, it can be prepared using the method described in patent application 202511721078.7.

[0027] In one specific embodiment of the present invention, the cactus polysaccharide includes cactus polysaccharide A, cactus polysaccharide B, and cactus polysaccharide C;

[0028] Among them, the number average molecular weight of cactus polysaccharide A is above 100,000 Da, the number average molecular weight of cactus polysaccharide B is 10,000-100,000 Da, and the number average molecular weight of cactus polysaccharide C is below 10,000 Da.

[0029] The mass ratio of cactus polysaccharide A, cactus polysaccharide B and cactus polysaccharide C in the cactus polysaccharide is cactus polysaccharide A: cactus polysaccharide B: cactus polysaccharide C = (5-15): (50-70): (10-30).

[0030] The described cactus polysaccharide, composed of cactus polysaccharides of specific number-average molecular weights in a specific ratio, exhibits more comprehensive anti-irritant effects, along with high stability, superior skin feel, and better solubility. This cactus polysaccharide demonstrates a multi-dimensional anti-irritant mechanism, exhibiting a uniform spherical structure under microscopic observation (electron microscopy). The cactus polysaccharides of different number-average molecular weights are recombine rather than existing independently, thus demonstrating both the individual characteristics of each number-average molecular weight cactus polysaccharide and their synergistic effects in terms of anti-irritant efficacy.

[0031] In one specific embodiment of the present invention, the mass ratio of cactus polysaccharide A, cactus polysaccharide B, and cactus polysaccharide C in the prickly pear polysaccharide is cactus polysaccharide A: cactus polysaccharide B: cactus polysaccharide C = (5-15):(50-70):(10-30), which can be any numerical ratio within this range, such as 5:70:10, 10:60:20, 15:50:30, etc. The mass fraction of cactus polysaccharide A in this mass ratio can be any mass fraction from 5 to 15 parts, such as 5 parts, 6 parts, 7.5 parts, 9 parts, 10 parts, 11.4 parts, 12.8 parts, 14 parts, 14.3 parts, or 15 parts. The mass fraction of cactus polysaccharide B can be any mass fraction from 50 to 70 parts, such as 50 parts, 52.6 parts, 55 parts, 60 parts, 63 parts, 65 parts, 67.4 parts, 69 parts, or 70 parts. The mass fraction of cactus polysaccharide C can be any mass fraction between 10 and 30, such as 10, 12.1, 15, 16, 20, 24.7, 25, 27, or 30 parts.

[0032] In one specific embodiment of the present invention, the cactus polysaccharide A, cactus polysaccharide B and cactus polysaccharide C are prepared by enzymatic hydrolysis of cactus water extract with amylase.

[0033] In one specific embodiment of the present invention, the method for preparing the cactus polysaccharide includes enzymatically hydrolyzing the water extract of cactus with amylase, and then screening out cactus polysaccharide A with a number average molecular weight of more than 100,000 Da, cactus polysaccharide B with a number average molecular weight of 10,000-100,000 Da and cactus polysaccharide C with a number average molecular weight of less than 10,000 Da, and mixing cactus polysaccharide A, cactus polysaccharide B and cactus polysaccharide C evenly in a mass ratio of cactus polysaccharide A: cactus polysaccharide B: cactus polysaccharide C = (5-15):(50-70):(10-30).

[0034] This method is simple and does not require the introduction of exogenous components. After enzymatically hydrolyzing the cactus water extract with amylase, cactus polysaccharides with specific number-average molecular weights are screened and combined in a specific ratio to achieve the desired anti-irritant efficacy with superior safety. The applicant discovered that after hydrolyzing the cactus water extract with amylase, the resulting cactus polysaccharide materials with different number-average molecular weights exhibit excellent compatibility, facilitating the recombination of cactus polysaccharides with different number-average molecular weights into a homogeneous whole.

[0035] In one specific embodiment of the present invention, the mass ratio of cactus polysaccharide A, cactus polysaccharide B, and cactus polysaccharide C in the cactus polysaccharide is cactus polysaccharide A: cactus polysaccharide B: cactus polysaccharide C = (5-15):(50-70):(10-30), which can be any numerical ratio within this range, such as 5:70:10, 10:60:20, 15:50:30, etc. The mass fraction of cactus polysaccharide A in this mass ratio can be any mass fraction between 5 and 15 parts, such as 5 parts, 6 parts, 7.5 parts, 9 parts, 10 parts, 11.4 parts, 12.8 parts, 14 parts, 14.3 parts, or 15 parts. The mass fraction of cactus polysaccharide B can be any number of parts between 50 and 70, such as 50, 52.6, 55, 60, 63, 65, 67.4, 69, or 70 parts. The mass fraction of cactus polysaccharide C can be any number of parts between 10 and 30, such as 10, 12.1, 15, 16, 20, 24.7, 25, 27, or 30 parts.

[0036] In one specific embodiment of the present invention, the method for preparing the cactus water extract includes extracting the cactus stem with water at 40-70℃ for 1-3 hours.

[0037] In one specific embodiment of the present invention, the cactus stem can be first crushed and then extracted with water. The extraction temperature can be selected between 40-70℃, such as 40℃, 42℃, 48℃, 50℃, 55℃, 60℃, 63.6℃, ​​67.2℃ or 70℃. The extraction time can be 1-3 hours, such as 1 hour, 1.2 hours, 1.5 hours, 2 hours, 2.3 hours, 2.8 hours or 3 hours, depending on the extraction temperature, to ensure sufficient extraction.

[0038] In one specific embodiment of the present invention, the mass ratio of the cactus stem to water is cactus:water = 1:(10-40), such as 1:10, 1:12, 1:15, 1:20, 1:23, 1:26.5, 1:30, 1:31, 1:35 or 1:40, and the amount of water used is sufficient to fully extract the cactus stem.

[0039] In one specific embodiment of the present invention, the material is left to stand for 12-18 hours after extraction to allow the extract residue to precipitate.

[0040] In one specific embodiment of the present invention, the cactus water extract is decolorized and filtered before being enzymatically hydrolyzed.

[0041] In one specific embodiment of the present invention, the cactus water extract is decolorized using activated carbon. The amount of activated carbon can be determined according to the general decolorization process. For example, the amount of activated carbon is 0.5-2.0% of the material mass, such as 0.5%, 0.6%, 0.74%, 0.9%, 1.0%, 1.2%, 1.25%, 1.4% or 1.5% of the material mass.

[0042] In one specific embodiment of the present invention, after decolorization, filtration is performed, for example, by using a paperboard filter to remove activated carbon and some impurities.

[0043] In one specific embodiment of the present invention, the amylase includes α-amylase.

[0044] In one specific embodiment of the present invention, the amount of amylase used is preferably sufficient to fully decompose the starch in the material. For example, the amount of amylase used in the enzymatic hydrolysis is 0.005%-0.02% of the material mass, such as 0.005%, 0.007%, 0.01%, 0.012%, 0.014%, 0.015%, 0.016%, 0.018%, 0.019%, or 0.02%.

[0045] In one specific embodiment of the present invention, the enzymatic hydrolysis temperature is preferably such that the amylase activity is ensured and the enzymatic hydrolysis reaction is fully carried out, for example, the enzymatic hydrolysis temperature is 50-55℃, such as 50℃, 51℃, 51.4℃, 52℃, 53℃, 54℃ or 55℃.

[0046] The enzymatic hydrolysis time is based on the standard that the enzymatic hydrolysis reaction is fully completed. The completion of the enzymatic hydrolysis reaction can be determined by sampling and detecting the starch content. For example, under the above conditions, the enzymatic hydrolysis time can be more than 30 minutes.

[0047] In one specific embodiment of the present invention, after enzymatic hydrolysis, the obtained material is desalted and then screened to obtain cactus polysaccharide A with a number average molecular weight of more than 100,000 Da, cactus polysaccharide B with a number average molecular weight of 10,000-100,000 Da, and cactus polysaccharide C with a number average molecular weight of less than 10,000 Da.

[0048] In one specific embodiment of the present invention, the desalination is carried out using cationic resin and anionic resin. The material can be desalinated by sequentially passing through cationic resin and anionic resin.

[0049] In one specific embodiment of the present invention, the screening is performed using membrane separation. Materials with a number-average molecular weight of 100,000 Da or higher are selected as cactus polysaccharide A, materials with a number-average molecular weight of 10,000-100,000 Da are selected as cactus polysaccharide B, and materials with a number-average molecular weight of less than 10,000 Da are selected as cactus polysaccharide C. Filter membranes with a number-average molecular weight of 100,000 Da and 10,000 Da can be selected. Materials collected on the 100,000 Da filter membrane are collected as cactus polysaccharide A, materials between the 100,000 Da and 10,000 Da filter membranes are collected as cactus polysaccharide B, and materials passing through the 10,000 Da filter membrane are collected as cactus polysaccharide C.

[0050] In one specific embodiment of the present invention, after mixing evenly, an adsorbent is added for filtration, and the fine filtrate is collected. Impurities contained therein can be further removed by the adsorbent, and the turbidity of the polysaccharide solution can be reduced as needed. Since polysaccharides have good solubility, the adsorbent is adsorbed and then the adsorbent is washed, which generally does not cause the loss of polysaccharides.

[0051] In one specific embodiment of the present invention, the adsorbent may be diatomaceous earth.

[0052] In one specific embodiment of the present invention, the mixture is further compounded after being thoroughly mixed.

[0053] In one specific embodiment of the present invention, glycerol is selected for compounding, and glycerol-water is used as the solvent system, which is more conducive to ensuring the stability of the material and can promote the recombination of cactus polysaccharides with different number average molecular weights. The mass ratio of material to glycerol is material:glycerol = 1:(0.5-1.5), such as 1:0.5, 1:0.6, 1:0.72, 1:0.9, 1:1, 1:1.1, 1:1.3 or 1:1.5.

[0054] In one specific embodiment of the present invention, the materials may be appropriately concentrated before compounding to reduce the amount of glycerin used; for example, concentrated to 1 / 2 of the original mass.

[0055] In one specific embodiment of the present invention, a preservative is added to the compounded material. The amount of the preservative should be determined according to the type of preservative and relevant national regulations and standards. For example, the amount of preservative is 1%-3% of the total mass of the material, such as 1%, 1.2%, 1.8%, 2%, 2.25%, 2.5%, 2.7% or 3%.

[0056] In one specific embodiment of the present invention, the preservative includes pentylene glycol / hexanediol.

[0057] In one specific embodiment of the present invention, the final material is sterilized to obtain cactus polysaccharide.

[0058] In one specific embodiment of the present invention, the sterilization includes sterilization at 95-100°C for more than 40 minutes.

[0059] In one specific embodiment of the present invention, the method for preparing the dextran includes: enzymatically hydrolyzing a material containing dextran using an endonuclease that specifically treats 1,4-glycosidic bonds to obtain a dextran product, wherein the ratio of 1,4-glycosidic bonds to 1,3-glycosidic bonds in the dextran product is 1.8 or less. Preferably, it can be prepared using the method described in patent application 202510766508.0.

[0060] The present invention uses an endonuclease that specifically treats 1,4-glycosidic bonds to prepare dextran, which helps to improve intramolecular and intermolecular aggregation of dextran molecules and increase its room temperature stability. The resulting dextran product can be added in large quantities to cosmetics and has a stronger anti-inflammatory effect. When added to cosmetics, it promotes a significant skin-soothing effect.

[0061] In one specific embodiment of the present invention, the material containing dextran is preferably a material from which cellulose has been removed. In the presence of cellulose, cellulase will preferentially react with cellulose, thereby affecting the action of cellulase on dextran.

[0062] In one specific embodiment of the present invention, the dextran includes β-glucan, a natural product that is a polysaccharide composed of linked glucose molecules and is found in various plants and fungi. β-glucan possesses many important biological activities and pharmacological effects and is widely used in medicine, health products, food, and other fields.

[0063] In one specific embodiment of the present invention, the dextran suitable for use in the present invention can be derived from different sources, including cereal β-glucan. All cereal β-glucans are unbranched, linear, non-starch polysaccharides composed of glucose linked by β-1,3 and β-1,4 glycosidic bonds. Therefore, different cereal β-glucans have similar properties, namely, they all exhibit instability due to hydrogen bonding.

[0064] In one specific embodiment of the present invention, the dextran includes one or more of oat β-glucan, barley β-glucan, and barley β-glucan.

[0065] In one specific embodiment of the present invention, the endonuclease specifically treating 1,4 glycosidic bonds refers to an endonuclease that has enzymatic activity only on 1,4 glycosidic bonds and no enzymatic activity on other types of glycosidic bonds in dextran (such as 1,3 glycosidic bonds, etc.). Commercially available endonucleases that meet the above requirements can be used, including one or more of cellulases (such as Novozymes complex cellulase, C0057 cellulase, etc.), mannanase, and dextranase.

[0066] In one specific embodiment of the present invention, the enzymatic hydrolysis time is 30-60 min, and the enzymatic hydrolysis time is preferably sufficient to allow the enzymatic hydrolysis reaction to proceed. For example, it can be exemplarily 30 min, 35 min, 40 min, 44 min, 47 min, 50 min, 56 min, or 60 min.

[0067] In one specific embodiment of the present invention, the enzymatic hydrolysis temperature is 50-60℃, preferably the suitable activity temperature of the cellulase, such as 50℃, 52℃, 55℃, 56℃, 58℃, or 60℃.

[0068] In one specific embodiment of the present invention, the material containing dextran includes a dextran extract. The dextran can be obtained from various sources through conventional extraction methods, or it can be purchased directly.

[0069] In one specific embodiment of the present invention, the material containing dextran is obtained by enzymatic extraction of plant raw materials containing dextran, or by dissolving raw materials containing dextran.

[0070] In one specific embodiment of the present invention, the material containing glucan is obtained by enzymatic extraction of plant raw materials containing glucan. One or more combinations of various enzymes disclosed in the prior art for enzymatic hydrolysis of plant raw materials containing glucan can be used, such as alkaline protease, etc. Cellulase can also be used in combination to decompose the cellulose contained in the plant raw materials.

[0071] In one specific embodiment of the present invention, during the enzymatic extraction, the mass ratio of the plant material containing dextran to water is 1:(10-20), preferably 1:15.

[0072] Depending on the actual equipment and the required concentration of the target product, different mass ratios of plant raw materials containing dextran to water can be used. Within the aforementioned mass ratio range of dextran-containing materials to water, the requirements for the enzymatic hydrolysis reaction of dextran can be met. For example, in different embodiments, the mass ratio of dextran-containing materials to water can be 1:10, 1:12.5, 1:14, 1:15, 1:16, 1:18, 1:20, etc.

[0073] In one specific embodiment of the present invention, when dissolving the raw material containing dextran, the mass ratio of the raw material containing dextran to water is 1:(12-70), preferably 1:14.

[0074] Depending on the actual equipment and the required concentration of the target product, the subsequent enzymatic hydrolysis of dextran can be satisfied within the above-mentioned mass ratio range of raw materials containing dextran to water. For example, in different embodiments, the mass ratio of raw materials containing dextran to water can be 1:12, 1:12.5, 1:14, 1:18, 1:20, 1:26, 1:34, 1:37, 1:42, 1:50, 1:53, 1:62, 1:69, 1:70, etc.

[0075] In one specific embodiment of the present invention, the temperature of the enzymatic hydrolysis is 70-90℃, preferably 80-85℃; the optimal activity temperature of the selected enzyme, such as alkaline protease, is preferred, for example, 70-73℃, 74-76℃, 78-80℃, 81-83℃, 88-90℃, etc.

[0076] In one specific embodiment of the present invention, the pH of the enzymatic extraction is preferably the pH suitable for the activity of the selected enzyme, such as alkaline protease.

[0077] In one specific embodiment of the present invention, the temperature for dissolving the raw material containing dextran is 70-90°C, preferably 80-85°C; it is preferable to facilitate the dissolution of the raw material containing dextran, and there is no strict temperature requirement. For example, it can be 70-73°C, 74-76°C, 78-80°C, 81-83°C, 88-90°C, etc.

[0078] In one specific embodiment of the present invention, the plant raw material containing glucan includes plants with a high glucan content, such as grains, preferably one or more of oats, barley and barley.

[0079] In one specific embodiment of the present invention, the plant raw material containing dextran includes the portion containing a large amount of dextran, such as plant bran.

[0080] In one specific embodiment of the present invention, the preparation method further includes at least one of decolorization, desalting, and protein removal. Other appropriate processing methods can be selected according to actual needs, such as the requirements of subsequent formulation applications.

[0081] In one specific embodiment of the present invention, the decolorization is performed 1-3 times, preferably 2 times.

[0082] In one specific embodiment of the present invention, the total amount of decolorizing agent added is 2%-5% of the total mass of the material to be decolorized, and more preferably 2-3%.

[0083] The total amount of decolorizing agent added can be determined according to the color of the obtained product and the decolorization requirements. The use of conventional decolorizing agents does not affect the properties of dextran. For example, in different embodiments, the total amount of decolorizing agent added can be 2%, 2.1%, 2.4%, 2.9%, 3.15%, 3.6%, 4.2%, 4.74%, 5% of the total mass of the material to be decolorized.

[0084] In one specific embodiment of the present invention, the decolorizing agent includes activated carbon.

[0085] In one specific embodiment of the present invention, the decolorization time for each decolorization is 30-60 minutes.

[0086] In different implementations, the time for each decolorization cycle can be exemplarily 30 min, 34 min, 39 min, 43 min, 46 min, 52 min, 57 min, 60 min, etc.

[0087] In one specific embodiment of the present invention, the temperature for each decolorization is 75-85°C.

[0088] The decolorization temperature can be set according to the requirements of equipment, production line, decolorization efficiency, etc. The decolorization temperature does not affect the properties of dextran. For example, in different embodiments, the decolorization temperature for each decolorization can be 75-77℃, 76-78℃, 78-80℃, 78.5-81℃, 83-85℃, etc.

[0089] In one specific embodiment of the present invention, the decolorization is performed twice. The first time, 3% of the total mass of the material to be decolorized is added as a decolorizing agent, and the decolorization is carried out at 80-85°C for 30 minutes. The second time, 2% of the total mass of the material to be decolorized is added as a decolorizing agent, activated carbon, and the decolorization is carried out at 80-85°C for 30 minutes.

[0090] In one specific embodiment of the present invention, anion and cation exchange resins are used for desalination.

[0091] In one specific embodiment of the present invention, the material flow rate during the desalination process is 1-3 t / h, and more preferably, the material flow rate during the desalination process is 2-3 t / h.

[0092] The material flow rate during the desalination process can be set according to the requirements of equipment, production line, desalination efficiency, etc. The material flow rate does not affect the properties of dextran. For example, in different embodiments, the material flow rate during the desalination process can be 1-1.3 t / h, 1.2-1.6 t / h, 1.9-2.4 t / h, 2-2.5 t / h, 2.3-2.7 t / h, 2.8-3 t / h, etc.

[0093] By combining isoelectric point treatment with heat treatment, trace amounts of protein in the sample can be destroyed, denatured, and precipitated, then removed by subsequent filtration steps, thereby improving the appearance and stability of the product as needed. This invention can also use other protein removal processes as alternatives, such as the Sevage method, TCA method, and protease method, depending on product and process requirements.

[0094] In one specific embodiment of the present invention, the obtained product is filtered and then compounded to obtain a dextran product.

[0095] In one specific embodiment of the present invention, one or more of hexanediol, pentanediol, glycerol and PHEG are used as compounding solvents for compounding.

[0096] In one specific embodiment of the present invention, the amount of the compound solvent can be adjusted as needed. In a preferred specific embodiment of the present invention, the obtained dextran is added to glycerol, hexanediol and pentanediol, and the glycerol content in the obtained product is 10% of the total mass, the hexanediol content is 2% of the total mass, and the pentanediol content is 2% of the total mass.

[0097] In one specific embodiment of the present invention, the compounding process is followed by sterilization.

[0098] Another aspect of the present invention provides a method for preparing a composition with heat protection effect, comprising: mixing cactus polysaccharide, dextran, ectoine and carnosine evenly. This method is simple and suitable for industrial production.

[0099] In one specific embodiment of the present invention, cactus polysaccharide and dextran are mixed and heated to 40-60°C, such as 40°C, 42°C, 43°C, 45°C, 47°C, 50°C, 53°C, 55°C, 58°C, 60°C, etc., and stirred for 0.5-1h, such as 0.5h, 0.6h, 0.7h, 0.8h, 0.9h, 1h, etc.; then ectoine and carnosine are added to the cactus polysaccharide and dextran mixture, and stirring is continued for 0.5-1h, such as 0.5h, 0.6h, 0.7h, 0.8h, 0.9h, 1h, etc., until the mixture is homogeneous.

[0100] In one specific embodiment of the present invention, the mixture is thoroughly mixed, filtered, and the filtrate is collected and sterilized.

[0101] In one specific embodiment of the present invention, the sterilization temperature is 80-90℃, such as 80℃, 81℃, 82℃, 83℃, 84℃, 85℃, 86℃, 87℃, 88℃, 89℃, 90℃, etc., and the sterilization time is 30-50min, such as 30min, 32min, 35min, 38min, 40min, 41min, 43min, 45min, 47min, 49min, 50min, etc.

[0102] Another aspect of the present invention provides the use of the composition with the above-mentioned heat protection effect in the preparation of cosmetics and / or pharmaceuticals.

[0103] The above-mentioned oily skin care composition has excellent heat protection effect and has broad application prospects in cosmetics and / or pharmaceuticals with anti-heat aging requirements.

[0104] Example 1

[0105] Compositions with thermal protection properties include:

[0106] Take 60g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared using the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights) and 35g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0) and mix them. Heat to 50℃ and stir for 1 hour. Add 4g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture and continue stirring for 1 hour until it is evenly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40 minutes.

[0107] Example 2

[0108] Compositions with thermal protection properties include:

[0109] Take 50g of cactus polysaccharide (prepared using the method described in Example 1 of patent application 202511721078.7) and 45g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0) and mix them together. Heat the mixture to 50℃ and stir for 1 hour. Add 4g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture and continue stirring for 1 hour until it is evenly dissolved. Filter the mixture using H70 paperboard, collect the filtrate, and sterilize it at 85℃ for 40 minutes.

[0110] Example 3

[0111] Compositions with thermal protection properties include:

[0112] Take 50g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared using the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights) and 45g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0) and mix them together. Heat to 50℃ and stir for 1 hour. Add 1g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture and continue stirring for 1 hour until it is evenly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40 minutes.

[0113] Example 4

[0114] Compositions with thermal protection properties include:

[0115] Take 70g of cactus polysaccharide (prepared using the method described in Example 1 of patent application 202511721078.7) and 25g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0) and mix them together. Heat the mixture to 50℃ and stir for 1 hour. Add 4g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture and continue stirring for 1 hour until it is evenly dissolved. Filter the mixture using H70 paperboard, collect the filtrate, and sterilize it at 85℃ for 40 minutes.

[0116] Example 5

[0117] Compositions with thermal protection properties include:

[0118] Take 70g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared using the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights) and 25g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0) and mix them. Heat to 50℃ and stir for 1h. Add 1g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture and continue stirring for 1h until it is evenly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40min.

[0119] Example 6

[0120] Take 55g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared using the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights) and 30g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0) and mix them. Heat to 50℃ and stir for 1 hour. Add 2g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture and continue stirring for 1 hour until it is evenly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40 minutes.

[0121] Comparative Example 1

[0122] Take 60g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared by the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights) and 40g of pure water, mix them, heat to 50℃, stir for 1h, and sterilize at 95℃ for 40min.

[0123] Comparative Example 2

[0124] Take 35g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared by the method described in patent application 202510766508.0) and 65g of pure water, mix them, heat to 50℃, stir for 1h, and sterilize at 95℃ for 40min.

[0125] Comparative Example 3

[0126] Take 4g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 96g of pure water, mix them, heat to 50℃, stir for 1h, filter using H70 paperboard, collect the filtrate, and sterilize at 95℃ for 40min.

[0127] Comparative Example 4

[0128] Take 1 part of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) and 99 parts of pure water, mix them, heat to 50℃, stir for 1 hour, filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40 minutes.

[0129] Comparative Example 5

[0130] Take 60g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared using the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights) and 35g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0) and mix them. Heat to 50℃ and stir for 1 hour. Add 4g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) to the mixture and continue stirring for 1 hour until it is evenly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40 minutes.

[0131] Comparative Example 6

[0132] Take 60g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared using the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights) and 35g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0) and mix them. Heat to 50℃ and stir for 1 hour. Add 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture and continue stirring for 1 hour until it is evenly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40 minutes.

[0133] Comparative Example 7

[0134] Take 60g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared using the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights) and 35g of pure water, mix them, heat to 50℃, and stir for 1h. Add 4g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture, and continue stirring for 1h until it is evenly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40min.

[0135] Comparative Example 8

[0136] Take 35g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared according to the method described in patent application 202510766508.0) and 60g of pure water, mix them, heat to 50℃, and stir for 1h. Add 4g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture, and continue stirring for 1h until it is evenly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40min.

[0137] Comparative Example 9

[0138] Take 40g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared using the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights) and 45g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0), heat to 50℃, and stir for 1h. Add 4g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture, and continue stirring for 1h until uniformly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40min.

[0139] Comparative Example 10

[0140] Take 70g of cactus polysaccharide (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Xianzhidun, prepared using the method described in patent application 202511721078.7, obtained by combining cactus polysaccharides of different molecular weights), and 20g of oat β-glucan (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Maishutong, prepared using the method described in patent application 202510766508.0), heat to 50℃, and stir for 1h. Add 4g of ectoine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 96702-03-3) and 1g of carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) to the mixture, and continue stirring for 1h until uniformly dissolved. Filter using H70 paperboard, collect the filtrate, and sterilize at 85℃ for 40min.

[0141] Comparative Example 11

[0142] The same formulation and steps as in Example 1 were used, the only difference being that the cactus polysaccharide extract was obtained by direct extraction of polysaccharides, without the combination of polysaccharides of different molecular weights. The extraction process of the cactus polysaccharide extract is as follows:

[0143] Weigh out cactus stem powder and add it to purified water at a material-to-liquid ratio (m / m) of 1:20; heat to 50℃, stir and extract for 2 hours, then let stand for 16 hours. Extract the supernatant, add 1.0% activated carbon (by weight of the material), keep warm at 80℃ for 1 hour, filter, and collect the filtrate; add 0.01% α-amylase (by weight of the filtrate), keep warm at 50℃ for 30 minutes; pass the enzymatic hydrolysate through cation exchange resin 001*7 and anion exchange resin D309 to obtain a desalted solution; concentrate the desalted solution to obtain a concentrate with a mass of 1 / 2 the mass of the aforementioned desalted solution; mix the concentrate and glycerol at a mass ratio of 1:1, add 2% hexanediol (by weight of the total product), mix well, and sterilize at 100℃ for 30 minutes to obtain the cactus extract.

[0144] Comparative Example 12:

[0145] The same proportions and steps as in Example 1 were used, except that the oat β-glucan extract (prepared using the method of Comparative Example 1 in patent application 202510766508.0) was not subjected to endonuclease hydrolysis of 1,4-glycosidic bonds.

[0146] Experimental Example 1: MMP-1 Inhibition Test

[0147] Test method:

[0148] (1) Inoculation: Inoculate cells into 24-well plates and incubate at 37°C in a 5% CO2 incubator for 18-24 hours.

[0149] (2) Sample feeding: After the cells in the 24-well plate have grown for 18-24 hours, samples are fed to the groups, with 3 replicates per group. Cell culture medium is added to the blank control and negative control groups, while cell culture medium containing 0.5 wt% of the samples from each example and comparative example is added to the sample groups. The samples are then cultured at 37°C in a 5% CO2 incubator for 18-24 hours.

[0150] (3) Thermal damage induction: According to the experimental group, the blank control group continued to be cultured at 37℃; the negative control group and the sample group were induced at 43℃ for 2h, and then cultured at 37℃ in a 5% CO2 incubator for 18-24h.

[0151] (4) MMP-1 content detection: Take the supernatant and use an ELISA kit for detection.

[0152] Experimental results:

[0153] Test results are as follows Figure 1 As stated above. By Figure 1 It is understood that the thermal protection composition of the present invention has a more significant inhibitory effect on MMP-1 compared with each comparative example. That is, the cactus polysaccharide, glucan, ectoine and carnosine combined in a specific ratio have a significant synergistic effect in inhibiting MMP-1. At the same time, compared with commercially available ordinary cactus polysaccharide and oat β-glucan, the specific cactus polysaccharide (combination of polysaccharides with different molecular weights) and oat β-glucan (flexible β-glucan) of this application have a more significant inhibitory effect on MMP-1, and the synergistic effect is more significant.

[0154] Experimental Example 2: TRPV1 Inhibition Test

[0155] Test method:

[0156] (1) Inoculation: Inoculate cells into 24-well plates and incubate at 37°C in a 5% CO2 incubator for 18-24 hours.

[0157] (2) Capsaicin stimulation: After cells were cultured in 24-well plates for 18-24 hours, they were divided into groups and administered the drug. Each group had 3 replicates. Cell culture medium was added to the blank control group, and cell culture medium containing 0.01 wt% capsaicin was added to the negative control group, positive control group and sample group. The cells were then cultured at 37°C in a 5% CO2 incubator for 18-24 hours.

[0158] (3) Sample feeding: After the cells in the 24-well plates have grown for 18-24 hours, samples are fed to the groups, with 3 replicates per group. Cell culture medium is added to both the blank control and the negative control group. Cell culture medium containing 0.001 wt% 4-tert-butylcyclohexanol is added to the positive control group. Cell culture medium containing 0.5 wt% of the samples from each example and comparative example is added to the sample groups. The cells are then cultured at 37°C in a 5% CO2 incubator for 18-24 hours.

[0159] (4) TRPV1 detection: On the fourth day, immunofluorescence staining was performed. The sample was washed three times with PBS, fixed with PBS buffer containing 4 wt% paraformaldehyde for 30 min, washed three times with PBS, permeabilized with PBS buffer containing 0.5 wt% Triton X-100 for 20 min, washed three times with PBS, blocked with PBS buffer containing 5 wt% BSA at room temperature for 60 min, discarded after blocking, and incubated overnight at 4°C with the prepared primary antibody. On the fifth day, the sample was washed three times with PBS, incubated with the prepared secondary antibody at room temperature in the dark for 1 h, washed three times with PBS, and an anti-fluorescence quencher was added. The sample was then observed and photographed using a fluorescence microscope.

[0160] (5) Data processing: Perform fluorescence photo processing to calculate IOD value.

[0161] Experimental results:

[0162] Experimental results are as follows Figure 2 As shown, by Figure 2 It is understood that the thermal protection composition of the present invention has a more significant inhibitory effect on TRPV-1 expression compared with each comparative example. That is, the cactus polysaccharide, glucan, ectoin and carnosine combined in a specific ratio have a significant synergistic effect in inhibiting TRPV-1 expression. At the same time, compared with commercially available ordinary cactus polysaccharide and oat β-glucan, the specific cactus polysaccharide (combination of polysaccharides with different molecular weights) and oat β-glucan (flexible β-glucan) of this application have a more significant inhibitory effect on TRPV-1 expression, and the synergistic effect is more significant.

[0163] Experimental Example 3:

[0164] Infrared irradiation of the skin to a certain extent can cause skin heating and redness, and prolonged irradiation can lead to photoaging. This experiment used thermal infrared irradiation to damage the skin, causing heating and redness. After the samples were treated, a skin sensitivity tester (TiVi 700) was used to test and evaluate whether the samples had the effect of reducing skin temperature and skin sensitivity, thereby evaluating the thermal protection efficacy of the samples.

[0165] (1) First, use an infrared lamp to irradiate the arm for 4 minutes. After removing the lamp, quickly find a position with uniform redness, measure the skin temperature and take a picture. Record the initial values ​​of skin temperature and skin sensitivity index.

[0166] (2) Apply a mixture of 2wt% sample gel from each embodiment and a gel matrix control to the test area.

[0167] (3) Skin temperature was measured at 0.5 min, 1.5 min, 3 min, 5 min and 10 min after the sample was applied; the skin sensitivity tester was used to collect images of the skin under polarized light in the test area at 10 min, 15 min and 20 min after the sample was applied, and the average skin sensitivity was obtained by image analysis.

[0168] The experimental results are shown in Figure 3 and Figure 4 ,Depend on Figure 3 , Figure 4 It is known that the thermal protection composition of the present invention can effectively improve the skin temperature rise and skin sensitivity and redness caused by heat damage, and has a good thermal protection effect.

[0169] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition with thermal protection function, characterized in that, Based on parts by mass, it includes the following components: 50-70 parts cactus polysaccharide, 25-45 parts glucan, 1-4 parts ectoine, and 1 part carnosine.

2. The composition with thermal protection function according to claim 1, characterized in that, The cactus polysaccharides include cactus polysaccharide A, cactus polysaccharide B, and cactus polysaccharide C; Among them, the number average molecular weight of cactus polysaccharide A is above 100,000 Da, the number average molecular weight of cactus polysaccharide B is 10,000-100,000 Da, and the number average molecular weight of cactus polysaccharide C is below 10,000 Da. The mass ratio of cactus polysaccharide A, cactus polysaccharide B and cactus polysaccharide C in the cactus polysaccharide is cactus polysaccharide A: cactus polysaccharide B: cactus polysaccharide C = (5-15): (50-70): (10-30).

3. The composition with thermal protection function according to claim 2, characterized in that, The cactus polysaccharide A, cactus polysaccharide B, and cactus polysaccharide C were prepared by enzymatic hydrolysis of cactus water extract with amylase, respectively. Preferably, the method for preparing the cactus polysaccharide includes: enzymatically hydrolyzing the water extract of cactus with amylase, and then screening out cactus polysaccharide A with a number average molecular weight of more than 100,000 Da, cactus polysaccharide B with a number average molecular weight of 10,000-100,000 Da and cactus polysaccharide C with a number average molecular weight of less than 10,000 Da, and mixing cactus polysaccharide A, cactus polysaccharide B and cactus polysaccharide C evenly in a mass ratio of cactus polysaccharide A: cactus polysaccharide B: cactus polysaccharide C = (5-15):(50-70):(10-30).

4. The oily skin care composition according to claim 3, characterized in that, The method for preparing the cactus water extract includes extracting the cactus stem with water at 40-70℃ for 1-3 hours. Preferably, the mass ratio of the cactus stem to water is cactus:water = 1:(10-40). Preferably, the sample is left to stand for 12-18 hours after extraction; Preferably, the cactus water extract is decolorized and filtered before being enzymatically hydrolyzed; Preferably, the cactus water extract is decolorized using activated carbon at a dosage of 0.5-2.0% of the material mass. Preferably, the amylase includes α-amylase; Preferably, the amount of amylase used in the enzymatic hydrolysis is 0.005%-0.02% of the material mass; Preferably, the enzymatic hydrolysis temperature is 50-55℃; Preferably, after enzymatic hydrolysis, the obtained material is desalted and then screened to obtain cactus polysaccharide A with a number average molecular weight of more than 100,000 Da, cactus polysaccharide B with a number average molecular weight of 10,000-100,000 Da, and cactus polysaccharide C with a number average molecular weight of less than 10,000 Da. Preferably, the desalination is carried out using cationic resin and anionic resin; Preferably, the screening is carried out by membrane separation, and materials with a number average molecular weight of more than 100,000 Da are selected as cactus polysaccharide A, materials with a number average molecular weight of 10,000-100,000 Da are selected as cactus polysaccharide B, and materials with a number average molecular weight of less than 10,000 Da are selected as cactus polysaccharide C. Preferably, the mixture is compounded after being thoroughly mixed; Preferably, the compound is made of glycerol, and the mass ratio of the material to the amount of glycerol is material:glycerol = 1:(0.5-1.5). Preferably, a preservative is added after compounding, and the amount of preservative is 1%-3% of the total mass of the materials; Preferably, the preservative includes pentylene glycol / hexanediol. Preferably, the final material is sterilized to obtain cactus polysaccharide; Preferably, the sterilization includes sterilization at 95-100°C for more than 40 minutes.

5. The composition with thermal protection function according to claim 1, characterized in that, The method for preparing the dextran includes: A dextran product is obtained by enzymatic hydrolysis of materials containing dextran using an endonuclease that specifically treats 1,4-glycosidic bonds, wherein the ratio of 1,4-glycosidic bonds to 1,3-glycosidic bonds in the dextran product is less than 1.

8.

6. The composition with thermal protection function according to claim 5, characterized in that, The dextran includes β-glucan; Preferably, the dextran comprises cereal β-glucan; Preferably, the dextran includes one or more of oat β-glucan, barley β-glucan, and barley β-glucan; Preferably, the endonuclease that specifically treats 1,4-glycosidic bonds includes one or more of cellulase, mannanase, and dextranase. Preferably, the enzymatic hydrolysis time is 30-60 min; Preferably, the enzymatic hydrolysis temperature is 50-60℃; The dextran-containing material is obtained by enzymatic extraction of dextran-containing plant raw materials, or by dissolving dextran-containing raw materials. Preferably, the enzymatic extraction is performed using alkaline protease; Preferably, during the enzymatic extraction, the mass ratio of the plant material containing dextran to water is 1:(10-20), more preferably 1:15; Preferably, when dissolving the raw material containing dextran, the mass ratio of the raw material containing dextran to water is 1:(12-70), more preferably 1:14; Preferably, the enzymatic extraction temperature is 70-90℃, and more preferably 80-85℃; Preferably, the temperature for dissolving the raw material containing dextran is 70-90°C, and more preferably 80-85°C; The plant raw materials containing glucan include cereals, preferably one or more of oats, barley and barley; Preferably, the plant-based raw material containing dextran includes plant bran; The preparation method further includes at least one of decolorization, desalting, and protein removal; The decolorization process is performed 1-3 times, preferably 2 times; Preferably, the total amount of decolorizing agent added is 2%-5% of the total mass of the material to be decolorized, and more preferably 2-3%. Preferably, the decolorizing agent comprises activated carbon; Preferably, the decolorization time for each decolorization is 30-60 minutes; Preferably, the temperature for each decolorization step is 75-85℃; More preferably, the decolorization is performed twice. In the first step, 3% of the total mass of the material to be decolorized is added as a decolorizing agent, and the decolorization is carried out at 80-85℃ for 30 minutes. In the second step, 2% of the total mass of the material to be decolorized is added as a decolorizing agent (activated carbon), and the decolorization is carried out at 80-85℃ for 30 minutes. Preferably, anion and cation exchange resins are used for desalination; Preferably, the material flow rate during the desalination process is 1-3 t / h; more preferably, the material flow rate during the desalination process is 2-3 t / h. The obtained product was filtered and then compounded to obtain a dextran product. Preferably, one or more of hexanediol, pentanediol, glycerol, and PHEG are used as the compounding solvent; Preferably, the mixture is sterilized after compounding.

7. A method for preparing a composition with thermal protection effect as described in any one of claims 1-6, characterized in that, Mix cactus polysaccharide, glucan, ectoine and carnosine evenly.

8. The method for preparing a composition with thermal protection effect according to claim 1, characterized in that, include: Mix cactus polysaccharide and glucan, heat to 40-60℃, and stir for 0.5-1 hour; then add ectoine and carnosine to the cactus polysaccharide and glucan mixture, and continue stirring for 0.5-1 hour until the mixture is homogeneous. Preferably, the mixture is thoroughly mixed, filtered, and the filtrate is collected and sterilized. Preferably, the sterilization temperature is 80-90℃, and the sterilization time is 30-50 minutes.

9. The use of a composition with heat protection effect as described in any one of claims 1-6 or a composition with heat protection effect prepared by the method of claim 7 or 8 in the preparation of cosmetics and / or pharmaceuticals.

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