Microfluidic device

Abstract

In a microfluidic device, the main surface of the microfluidic device is protected. A microfluidic device (100) is provided with: a plate-like member (20); a protrusion (84) disposed on the plate-like member; and first protrusions (32, 34, 36) disposed on the plate-shaped member. The plate-like member has a substrate having a first surface and a second surface, and a plurality of microchannels (24) are formed on one of the surfaces. The protruding object and the first protruding part are arranged on the first surface of the substrate. A distance from the first surface to a farthest end of the first protrusion is greater than a distance from the first surface to a farthest end of the protrusion.

Description

Technical Field

[0001] This disclosure relates to plate-shaped microfluidic devices that can be stacked and stored. More specifically, it relates to techniques for protecting the main surface of microfluidic devices. Background Technology

[0002] Japanese Patent Application Publication No. 2023-54876 (Patent Document 1) discloses a microfluidic device with multiple microfluidic paths for testing the sensitivity of bacteria to antimicrobial drugs.

[0003] Existing technical documents Patent documents Patent Document 1: Japanese Patent Application Publication No. 2023-54876 Summary of the Invention The technical problem that the invention aims to solve Generally, when using microfluidic devices to test the susceptibility of bacteria to antimicrobial agents, a test fluid containing the sample is injected into the microfluidic path. After the sample interacts with the agent within the microfluidic path, the sample is observed through a microscope or similar means. Therefore, if the observed area on the main surface of the microfluidic device or functional components such as sensors are covered with scratches or dirt, the user may not be able to accurately observe the condition of the sample through a microscope or sensor.

[0004] However, when microfluidic devices are typically stacked for storage or distribution, the main surface of the microfluidic device, containing the observation area and functional components such as sensors, sometimes comes into direct contact with the microfluidic devices above or below it. This can lead to scratches or dirt adhering to the observation area on the main surface of the microfluidic device, potentially preventing users from accurately observing the condition of the subject using a microscope or similar instrument. Furthermore, scratches or dirt can also accumulate on the functional components such as sensors on the main surface of the microfluidic device, further hindering users from accurately assessing the condition of the subject through these sensors.

[0005] This disclosure was made to solve the above-mentioned problems, and its purpose is to protect the main surface of the microfluidic device in the microfluidic device.

[0006] Solution to the above technical problems A microfluidic device according to one aspect of this disclosure includes: a plate-shaped member; a protrusion disposed on the plate-shaped member; and a first protrusion disposed on the plate-shaped member. The plate-shaped member has a substrate having a first surface and a second surface, on which a plurality of microfluidic paths are formed. The protrusion and the first protrusion are disposed on the first surface of the substrate. The distance from the first surface to the farthest end of the first protrusion is greater than the distance from the first surface to the farthest end of the protrusion.

[0007] Invention Effects In the microfluidic device disclosed herein, a protrusion (rib) is disposed on the main surface of the microfluidic device. Therefore, it is possible to prevent the main surface of the microfluidic device from contacting other microfluidic devices, thereby protecting the main surface of the microfluidic device. Attached Figure Description

[0008] 【 Figure 1 [Image 1] is a diagram showing the overall structure of a test apparatus using a microfluidic device according to an embodiment.

[0009] 【 Figure 2 This is a schematic diagram showing an example of the hardware structure of the control unit.

[0010] 【 Figure 3 [Illustration 1] is a diagram showing the overall structure of the microflow device according to the embodiment.

[0011] 【 Figure 4 The diagram is used to illustrate the microflow device of Modified Example 1.

[0012] 【 Figure 5 The diagram is used to illustrate the microflow device of Modified Example 2.

[0013] 【 Figure 6 The diagram is used to illustrate the microflow device of variation example 3.

[0014] 【 Figure 7 The diagram is used to illustrate the microflow device in variation example 4.

[0015] 【 Figure 8 The diagram is used to illustrate the microflow device of variation 5.

[0016] 【 Figure 9 The diagram shown is a detailed representation of the housing section, which serves as an example of other equipment.

[0017] 【 Figure 10 [] is used for explanation Figure 9 A diagram showing the structure of the bottom surface 3Z of the containment section.

[0018] 【 Figure 11 [] is used for explanation Figure 9 A diagram showing the structure of the bottom surface 5Z of the containment section. Detailed Implementation

[0019] Hereinafter, embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. Furthermore, the same or equivalent parts in the drawings will be labeled with the same reference numerals, and their descriptions will be omitted.

[0020] [Implementation Method] <Structure of the experimental apparatus> Figure 1This is a diagram showing the overall structure of a test apparatus 200 using the microfluidic device 100 according to an embodiment. The test apparatus 200 is used, for example, for pharmaceutical sensitivity testing.

[0021] The experimental setup 200 takes pictures of each of the multiple observation points set on the microfluidic device 100. Each observation point contains an object obtained by contacting a test solution containing bacteria as biological agents with a reagent.

[0022] The experimental apparatus 200 includes: a control unit 120, a microscope camera 140, a stage 160, a reading unit 180, an incubator 50, a receiving unit 101, an injection unit 103, a waste disposal unit 190, and a transport unit 500.

[0023] The stage 160 includes a field-of-view alteration mechanism 162 and an illumination device 164. A microfluidic device 100 is mounted on the stage 160. The illumination device 164 is a transmitted illumination device that shines light onto the microfluidic device 100 on the stage 160 for observation.

[0024] In the following description, the normal direction of the stage 160 on which the microfluidic device 100 is placed is referred to as the Z-axis direction. Furthermore, any direction perpendicular to the Z-axis direction is referred to as the X-axis direction. Additionally, the direction perpendicular to both the Z-axis and Y-axis directions and parallel to the main surface of the stage 160 on which the microfluidic device 100 is placed is referred to as the Y-axis direction.

[0025] Viewed from above along the positive Z-axis, the microfluidic device 100 is, for example, a generally rectangular plate extending in the XY plane, and also has height in the Z-axis direction. The microfluidic device 100 is disposed on the stage 160 with its long side extending along the X-axis and its short side extending along the Y-axis. Furthermore, the microfluidic device 100 can also be other shapes such as circular, elliptical, or polygonal.

[0026] The field-of-view alteration mechanism 162 alters the field of view of the microscope camera 140. The field-of-view alteration mechanism 162 includes an X-axis movement mechanism 162X and a Y-axis movement mechanism 162Y. The X-axis movement mechanism 162X moves the microfluidic device 100 mounted on the stage 160 in the X-axis direction. The Y-axis movement mechanism 162Y moves the microfluidic device 100 mounted on the stage 160 in the Y-axis direction.

[0027] The microscope camera 140 includes an objective lens 142, a focus adjustment mechanism 144, and an image sensor 146. The objective lens 142 magnifies a portion of the microflow device 100 disposed on the stage 160. The objective lens 142 can be selected at any magnification depending on the object being observed.

[0028] The focus changing mechanism 144 changes the focus of the microscope camera 140. For example, the focus changing mechanism 144 changes the focus of the microscope camera 140 by changing the position of the objective lens 142 in the direction of the optical axis of the objective lens 142.

[0029] Image sensor 146 is a detector used to capture an image of the observed object magnified by objective lens 142. Image sensor 146 is, for example, a CCD (Charge Coupled Device) image sensor and a CMOS (Complementary Metal Oxide Semiconductor) image sensor.

[0030] The reading unit 180 reads the identification information of the microflow path device 100. The reading unit 180 is, for example, a barcode reader, a QR code (registered trademark) reader, or a reader that can read RF (Radio Frequency) tags, depending on the type of identification code assigned to the microflow path device 100. The reading unit 180 sends the read identification information to the control unit 120.

[0031] The incubator 50 cultivates bacteria within the microfluidic device 100, which is housed within a container filled with test solution and kept at a suitable temperature for bacterial cultivation. The suitable temperature for bacterial cultivation is, for example, approximately 37 degrees Celsius.

[0032] The containment section 101 contains the brand-new microfluidic device 100 before the test fluid is injected. The injection section 103 injects the test fluid into the brand-new microfluidic device 100 before the test fluid is injected. The disposal section 190 contains the microfluidic device 100 that is no longer needed after observation and is considered as waste.

[0033] The conveying unit 500 includes: a fork (not shown) for placing and moving the microfluidic device 100; and a drive unit (not shown) for driving the fork. The fork constitutes an example of a holding part of a holding plate. In the conveying unit 500, the microfluidic device 100 can be moved towards the receiving part 101, the injection part 103, the incubator 50, the stage 160, and the waste part 190 by driving the fork via the drive unit. Furthermore, the microfluidic device 100 can be repeatedly moved between the incubator 50 and the stage 160 to observe the culture status of the bacteria over time. The drive unit is controlled by the control unit 120.

[0034] Based on the information read by the reading unit 180, the control unit 120 controls the microscope camera 140 and the stage 160 to capture images of the observation points of the microfluidic device 100. Furthermore, the control unit 120 controls the transport unit 500 to move the microfluidic device 100.

[0035] <Structure of the Control Department> Figure 2 This is a schematic diagram illustrating an example of the hardware structure of the control unit 120. The control unit 120 is configured, for example, according to a general computer architecture.

[0036] The control unit 120, as a main structural element, includes a processor 122, a memory 124, and an input / output interface (I / F) 126. These units are communicatively connected to each other via a bus 128.

[0037] The memory 124 is implemented using non-volatile memory such as RAM (Random Access Memory), ROM (Read Only Memory), and flash memory, or storage devices such as disks. The memory 124 stores programs executed by the processor 122, or data used by the processor 122.

[0038] Specifically, memory 124 stores identification information of the microfluidic device 100 obtained from the identification code assigned to the microfluidic device 100 and read from the reading unit 180. The identification information includes imaging conditions for photographing each observation point on the microfluidic device 100. The imaging conditions include: observation point information showing the position of the observation point as the subject of the photograph; and a focus position that aligns the focus of the microscope camera 140 with the observation point as the subject of the photograph.

[0039] The input / output I / F126 is an interface for exchanging various data between the focus changing mechanism 144, the image sensor 146, the shooting field of view changing mechanism 162, the reading unit 180, the conveying unit 500, and the control unit 120.

[0040] Specifically, the input / output I / F 126 receives identification information from the microfluidic device 100 from the readout unit 180. The input / output I / F 126 outputs observation point information, including the position of the observation point as the subject of the photograph, contained in the input identification information, to the field-of-view adjustment mechanism 162 of the stage 160. Furthermore, the input / output I / F 126 outputs the focus position corresponding to the observation point as the subject of the photograph, contained in the input identification information, to the focus adjustment mechanism 144 of the microscope camera 140.

[0041] Furthermore, the I / O input 1 / F 126 outputs a shooting instruction to the image sensor 146 of the microscope camera 140. Additionally, the I / O input 1 / F 126 receives image data from the image sensor 146 of the microscope camera 140. Furthermore, the I / O input 1 / F 126 outputs a transport instruction to the transport unit 500.

[0042] The processor 122 is typically an arithmetic processing unit such as a CPU (Central Processing Unit) or an MPU (Microprocessor). The processor 122 controls the operation of various parts of the experimental apparatus 200 by reading and executing programs stored in the memory 124.

[0043] Specifically, in order to include the observation point within the field of view, the processor 122 drives the field-of-view changing mechanism 162 to move the stage 160 according to the observation point information indicating the position of the observation point as the subject of the photograph. Furthermore, the processor 122 drives the focus changing mechanism 144 of the microscope camera 140 to move the microscope camera 140 to the focusing position.

[0044] Furthermore, the processor 122 uses the image sensor 146 of the microscope camera 140 to capture images of the observation points through which the microscope camera 140 is the subject. Then, the processor 122 drives the transport unit 500 to transport the microfluidic device 100 from the injection unit 103 or the incubator 50 to the stage 160 before observation. Then, the processor 122 drives the transport unit 500 to transport the microfluidic device 100 from the stage 160 to the waste unit 190 or the incubator 50 after observation. And, in Figure 2 In the example, a single processor is shown, but the control unit 120 can also be configured to have multiple processors.

[0045] <Structure of Microflow Devices> Figure 3 This is a diagram showing the overall structure of the microflow device 100 according to the embodiment. Figure 3 A perspective view of the microflow device 100 is shown. (See figure.) Figure 3 As shown, the microfluidic device 100 includes a plate-shaped component 20. The plate-shaped component 20 may be generally rectangular, or its edges and corners may be partially cut out. The plate-shaped component 20 includes a substrate 18 and a thin film 19. The substrate 18 has a flow path structure on its surface in the negative Z-axis direction. The thin film 19 covers the flow path structure formed on the negative Z-axis surface of the substrate 18 from the negative Z-axis direction.

[0046] The flow path structure includes: an opening 22, a main flow path 23, a microflow path 24, a storage section 25, an opening 26, a gas permeable membrane 27, a recovery section 28, and an opening 29. Furthermore, the microflow path device 100 may also have a structure without the opening 26.

[0047] An opening 22 is formed on one end of the main flow path 23 on the surface of the substrate 18 in the positive Z-axis direction and communicates with the main flow path 23. Test liquid is forced from the opening 22 into the main flow path 23 using fluid pressure. A hole is formed in the portion of the opening 22 protruding from the plate-shaped member 20 for injecting the test liquid into the microfluidic device 100 using a syringe or the like. The test liquid forced into the main flow path 23 is then forced into the microflow path 24 disposed in the Y-axis direction. In this embodiment, air pressure is used as the fluid pressure. The hole in the protruding portion of the opening 22 is, for example, formed in a circular shape. The diameter of the hole in the protruding portion of the opening 22 is, for example, 5 μm to 5 mm. In this embodiment, the opening 22 is formed at one end of a main flow path 23. The main flow path 23 is disposed at a position surrounding the outer side of multiple microflow paths 24.

[0048] The main flow path 23 has an inlet-side end 23A with an opening 22, and an outlet-side end 23B located on the opposite side from the inlet-side end 23A. Extending from the opening 22, the main flow path 23 branches into multiple microflow paths 24. The main flow path 23 is connected to the multiple microflow paths 24 in a manner that allows the test liquid to flow. The test liquid flowing in from the opening 22 passes through the main flow path 23 and flows into the branching microflow paths 24. The main flow path 23 and the microflow paths 24 have rectangular cross-sections, and their widths are, for example, 1 μm to 1 mm.

[0049] However, the dimensions of the main flow path 23 and the microflow path 24 in the Z-axis direction are different. For example, the dimension of the main flow path 23 in the Z-axis direction is 0.5 mm, while the dimension of the microflow path 24 in the Z-axis direction is as small as 0.025 mm. That is, the length of the Z-axis direction from the junction of the thin film 19 and the substrate 18 to the void at the bottom of the flow path is different in the main flow path 23 and the microflow path 24.

[0050] Therefore, the flow resistance of the microflow path 24 is greater than that of the main flow path 23. By making the flow resistance of the microflow path 24 greater than that of the main flow path 23, as described below, the test liquid flowing in from the opening 22 can almost simultaneously flow into multiple microflow paths 24 once it fills the main flow path 23.

[0051] In this embodiment, the 32 microflow paths 24 arranged side-by-side in the X-axis direction are designated as one group, and two groups are arranged side-by-side in the Y-axis direction. That is, the microflow path device 100 has a group 24P in the positive Y-axis direction and a group 24N in the negative Y-axis direction. Each of the multiple microflow paths 24 has: a first side end 24A communicating with the main flow path 23; and a second side end 24B located on the opposite side relative to the first side end 24A.

[0052] Each of the multiple microflow paths 24 included in group 24P is connected to the main flow path 23 arranged in the positive Y-axis direction of the microflow path device 100. Therefore, the test liquid branching off from the main flow path 23 flows in in the negative Y-axis direction.

[0053] On the other hand, each of the multiple microflow paths 24 included in group 24N is connected to the main flow path 23 disposed in the negative Y-axis direction of the microflow path device 100. Therefore, the multiple microflow paths 24 included in group 24N are disposed in the Y-axis direction, and the test liquid branching from the main flow path 23 flows in in the positive Y-axis direction.

[0054] A roughly rectangular storage section 25 is provided in the middle of each microfluidic path 24. Therefore, the test liquid flowing in from the opening 22 flows through the main flow path 23 and the first half 24F of the microfluidic path 24 to each storage section 25.

[0055] The storage section 25 is connected to the opening 22 via the main flow path 23, and stores the test solution flowing in from the opening 22. A reagent is pre-prepared in the storage section 25. That is, the reagent is placed in the storage section 25 before the test solution flows into it. The test solution reacts with the reagent in the storage section 25. The reagent is, for example, an antibacterial agent. The reagent can be solid or liquid.

[0056] exist Figure 3 In this example, 60 (30 x 2) storage compartments 25 are formed on the plate-shaped component 20. The volume of the test solution stored in each of the 60 storage compartments 25 is the same. On the other hand, the type and amount of the drug placed in the 60 storage compartments 25 may be the same or different from each other.

[0057] A rear half 24S of the microfluidic path is disposed between the storage section 25 and the opening 26. The rear half 24S of the microfluidic path is disposed along the Y-axis direction, with one end connected to the storage section 25 and the other end (second side end 24B) forming the opening 26. In the rear half 24S of the microfluidic path, the test liquid flowing into the storage section 25 flows to the opening 26.

[0058] An opening 26 is formed for discharging air from the main flow path 23 and the microflow path 24 to the outside of the microflow device 100 and for filling the second end 24B of the microflow path with test liquid. The opening 26 is, for example, circular when viewed from the positive Z-axis direction. The diameter of the opening 26 is, for example, 5 μm to 5 mm. The opening 26 is covered by a gas-permeable membrane 27. Specifically, in Figure 3 In this configuration, 30 openings 26 connected to multiple microflow paths 24 included in group 24P and 30 openings 26 connected to multiple microflow paths 24 included in group 24N are arranged in a mutually opposing manner.

[0059] Therefore, 60 (30 x 2) openings 26 are formed along the X-axis in the central portion of the microfluidic device 100. These 60 openings 26 are covered by a single gas-permeable membrane 27. Furthermore, the gas-permeable membrane 27 is not limited to covering all 60 openings 26 with a single membrane; it can also be divided into two membranes covering 30 openings 26 in group 24P and 30 openings 26 in group 24N. Additionally, the gas-permeable membrane 27 can cover at least one of the 60 openings 26.

[0060] The gas-permeable membrane 27 has the function of allowing gas to pass through but preventing liquid from passing through. Examples of materials for the gas-permeable membrane 27 include polytetrafluoroethylene (PTFE). The gas-permeable membrane 27 is preferably hydrophobic. The thickness of the gas-permeable membrane 27 is 1 mm or less. The gas-permeable membrane 27 is fixed to the plate-shaped component 20 by bonding with an adhesive, ultrasonic welding, or the like. Examples of adhesives include photocurable resins, thermocurable resins, and pressure-sensitive resins. By configuring the gas-permeable membrane 27, air present in the main flow path 23 and the microflow path 24 before the test can be released from the opening 26 to the outside of the microflow device 100, thus filling the main flow path 23 and the microflow path 24 with the test liquid, while preventing the test liquid from flowing out of the microflow device 100.

[0061] The main flow path 23, connected to the opening 22, is configured to surround the outside of the microflow path 24 and is connected to the recovery section 28. The recovery section 28 is located at the outlet end 23B of the main flow path 23. The recovery section 28 recovers a portion of the test fluid flowing into the main flow path 23 from the opening 22.

[0062] The recovery section 28 is formed in a cuboid shape. The length of one side of the recovery section 28 is, for example, 10 μm to 10 mm. A component for absorbing moisture, such as a sponge, may also be provided in the recovery section 28. This prevents backflow from the recovery section 28 to the main flow path 23 and also prevents the test solution from evaporating from the main flow path 23.

[0063] An opening 29 is formed on the surface of the substrate 18 in the positive Z-axis direction and is connected to the end of the recovery section 28. The test liquid can flow from the opening 22 to the opening 29 through the main flow path 23 and the recovery section 28. An opening 29 is formed for discharging the test liquid in the main flow path 23 and the recovery section 28 to the outside of the microflow device 100.

[0064] The opening 29 can be switched open or closed via a switch (not shown) on the test apparatus 200. By closing the opening 29, the test liquid flowing into the main flow path 23 can be prevented from being discharged to the recovery section 28 and the opening 29. On the other hand, by opening the opening 29, the test liquid remaining in the main flow path 23 can be discharged to the recovery section 28 for recovery.

[0065] Generally, when using microfluidic devices to test the sensitivity of bacteria to antimicrobial agents, a test fluid containing the sample is injected into the microfluidic path. After the sample interacts with the agent within the microfluidic path, the sample is observed through a microscope or similar means. Therefore, if the observed area on the main surface of the microfluidic device or functional components such as sensors are covered with scratches or dirt, the user may not be able to accurately assess the condition of the sample through the microscope or sensors.

[0066] However, when microfluidic devices are stacked on top of each other for storage or distribution, the main surface of the microfluidic device, which contains functional components such as the observed area and sensors, may sometimes come into direct contact with the microfluidic devices above or below it.

[0067] Therefore, the microfluidic device 100 of this disclosure also includes ribs 32, 34, and 36 protruding from the main surface. Rib 32 is disposed at the end of the plate-like member 20 in the negative X-axis direction, extending along the Y-axis direction. Figure 3 In the example, three ribs 32 are arranged on the same straight line in the Y-axis direction.

[0068] Furthermore, the rib 34 is configured at the end of the plate-shaped member 20 in the positive X-axis direction to extend along the Y-axis direction. Figure 3 In the example, three ribs 34 are arranged on the same straight line in the Y-axis direction. Ribs 32 and 34 are preferably at the same height in the Z-axis direction.

[0069] By configuring ribs 32 and 34, when multiple microfluidic devices 100 are stacked, only the surfaces of ribs 32 and 34 on the negative Z-axis side of other microfluidic devices 100 are in contact. Therefore, it is possible to prevent the main surface of microfluidic device 100 from contacting other microfluidic devices 100, and to protect the main surface of microfluidic device 100.

[0070] Furthermore, the main surfaces of most microfluidic devices are flat. When microfluidic devices are stacked on top of each other for storage before and during use, the main surfaces of the overlapping microfluidic devices sometimes overlap almost without gaps. This makes it difficult to grasp the microfluidic device individually, and thus it may be difficult to extract a single microfluidic device from a stack of multiple microfluidic devices.

[0071] By configuring ribs 32 and 34, gaps are created between the main surfaces of the microfluidic devices 100 when multiple microfluidic devices 100 are stacked, thus making it easier to extract the microfluidic devices one by one. In order to protect the opening 22 protruding from the plate-shaped member 20 from damage such as the corners of the microfluidic devices above when extracting the microfluidic devices, the microfluidic device 100 also has ribs 36.

[0072] Rib 36 includes: an arcuate portion surrounding the opening 22 on the main road 23, excluding the opening; and a portion extending from the arcuate portion in the negative Y-axis direction. The length of rib 36 in the Z-axis direction may be the same as or less than that of ribs 32 and 34.

[0073] Thus, by configuring ribs 32, 34, or 36, it is possible to prevent the main surface of the microfluidic device 100 from contacting other microfluidic devices 100 when multiple microfluidic devices 100 are stacked. Therefore, the main surface of the microfluidic device 100 can be protected.

[0074] (Variation Example 1) Figure 4 This is a diagram used to illustrate the microflow device 100A of Modified Example 1. Figure 4 The diagram shows a stacked state in the Z-axis direction of a microfluidic device 100A, with ribs disposed on the surface 20AT of the plate-shaped component 20A on the positive Z-axis side, viewed from the negative Y-axis direction. The microfluidic device 100A, in addition to possessing… Figure 3 In addition to its structure, the microfluidic device 100 also includes functional components and protrusions 84. The protrusions 84 are, for example, openings 22. The surface 20AB on the negative Z-axis side of the plate-like component 20A of the microfluidic device 100A is flat.

[0075] The functional components include a sensor 82 and RFID (Radio Frequency Identification). The RFID stores identification information for each microfluidic device 100A. This identification information may include, for example, the identification number of the microfluidic device 100A, or the type of drug pre-configured within the microfluidic device 100A. The identification information is also used to determine the number of stacked microfluidic devices 100A. Alternatively, identification information can be stored in barcodes or QR codes (registered trademarks) instead of RFID.

[0076] Hereinafter, the distance in the Z-axis direction from the surface 20AT of the rib on which the plate-shaped member is disposed to the end of the rib in the Z-axis direction will be referred to as the height of the rib. Similarly, the distance in the Z-axis direction from the surface of the sensor on which the plate-shaped member is disposed to the end of the sensor in the Z-axis direction will be referred to as the height of the sensor. Likewise, the distance in the Z-axis direction from the surface of the protrusion on which the plate-shaped member is disposed to the end of the protrusion in the Z-axis direction will be referred to as the height of the protrusion. Furthermore, the term "sensor" in the embodiment corresponds to "functional component" in this disclosure.

[0077] In the microfluidic device 100A, the height of rib 32 corresponds to the distance between the plate-shaped components 20A. The heights of ribs 32 and 34 are set such that the distance between the plate-shaped components 20A is greater than the height of the sensor 82 and the height of the protrusion 84. By doing so, the main surface of the microfluidic device 100A, which contains functional components such as the observed area and the sensor, can be prevented from contacting other microfluidic devices. Therefore, the main surface of the microfluidic device 100A can be protected.

[0078] Furthermore, when the microfluidic devices 100A are stacked on top of each other, gaps are created between adjacent microfluidic devices 100A. By doing so, the boundaries of each overlapping microfluidic device can be identified by a sensor, thus enabling automatic handling of individual microfluidic devices and automatic determination of the number of stacked microfluidic devices.

[0079] (Variation Example 2) Figure 5 This is a diagram used to illustrate the microflow device 100B of Modified Example 2. Figure 5 The diagram shows a stacked microfluidic device 100B with recesses 42 and 44 formed on the surface 20BB of the plate-shaped member 20B in the negative Z-axis direction, viewed from the negative Y-axis direction. The microfluidic device 100B has the structure of the microfluidic device 100A, but also has recesses 42 and 44. The surface 20BT of the plate-shaped member 20B of the microfluidic device 100B in the positive Z-axis direction has the same structure as the microfluidic device 100A.

[0080] In the microfluidic device 100B, ribs 32 are disposed on the surface 20BT of the plate-shaped member 20B, and recesses 42 are formed on the surface BB of the plate-shaped member 20B. Viewed from above along the Z-axis, the ribs 32 on the surface 20BT of the plate-shaped member 20B overlap with the recesses 42 on the surface 20BB of the plate-shaped member 20B. Similarly, corresponding to the ribs 34 disposed on the surface 20BT, recesses 44 are formed on the surface 20BB. By doing so, when the microfluidic device 100B is stacked, the ribs 32 are embedded in the recesses 42, and the ribs 34 are embedded in the recesses 44.

[0081] When the depth of the recess is defined as the distance in the Z-axis direction from the surface of the recess where the plate-shaped member is formed to the bottom of the recess in the Z-axis direction, in the microfluidic device 100B, the value of subtracting the depth of the recess 42 from the height of the rib 32 and the value of subtracting the depth of the recess 44 from the height of the rib 34 become the distance between the two stacked plate-shaped members 20. The heights of the ribs 32 and 34 and the depths of the recesses 42 and 44 are set such that the distance between the plate-shaped members 20 is greater than the height of the sensor 82 and the height of the protrusion 84.

[0082] By doing so, in the case of stacking multiple microflow devices 100B, it is possible to prevent the main surface of the microflow device 100B from contacting the main surface of the plate-shaped component in the adjacent microflow device 100B, thus protecting the main surface of the microflow device.

[0083] Furthermore, when the microfluidic devices 100B are stacked on top of each other, gaps are created between adjacent microfluidic devices 100B. By doing so, the boundaries of each overlapping microfluidic device can be identified by a sensor, thus enabling automatic handling of individual microfluidic devices and automatic determination of the number of stacked microfluidic devices.

[0084] (Variation Example 3) Figure 6 This is a diagram used to illustrate the microflow device 100C of Modified Example 3. Figure 6 The diagram shows a stacked microfluidic device 100C, viewed from the negative Y-axis direction, in which ribs 36 and 38 are arranged on the surface 20CB of the plate-shaped component 20C in the negative Z-axis direction, and recesses 46 and 48 are formed on the surface 20CT of the plate-shaped component 20 in the positive Z-axis direction. The surface of the plate-shaped component 20 in which the ribs and recesses are formed in the microfluidic device 100C is different from the surface of the plate-shaped component 20 in which the ribs and recesses are formed in the microfluidic device 100B.

[0085] The surface 20CT of the plate-shaped member 20C in the positive Z-axis direction of the microfluidic device 100C has the same structure as the surface 20CB of the plate-shaped member 20B in the negative Z-axis direction of the microfluidic device 100B. Furthermore, the surface 20CB of the plate-shaped member 20C in the negative Z-axis direction of the microfluidic device 100C has the same structure as the surface 20BT of the plate-shaped member 20B in the negative Z-axis direction of the microfluidic device 100B.

[0086] In the microfluidic device 100C, ribs 36 are disposed on the surface 20CB of the plate-shaped member 20C, and recesses 46 are formed on the surface 20CT of the plate-shaped member 20C. Viewed from above in the Z-axis direction, the ribs 36 on the surface 20CB of the plate-shaped member 20C overlap with the recesses 46 on the surface 20CT of the plate-shaped member 20C. Similarly, corresponding to the ribs 38 disposed on the surface 20CB, recesses 48 are formed on the surface 20CT. By doing so, when the microfluidic device 100C is stacked, the ribs 36 are embedded in the recesses 46, and the ribs 38 are embedded in the recesses 48.

[0087] In the microfluidic device 100C, the difference between the height of rib 36 and the depth of recess 46, and the difference between the height of rib 38 and the depth of recess 48, constitutes the distance between the two stacked plate-like components 20. The heights of ribs 36 and 38 and the depths of recesses 46 and 48 are set such that the distance between the plate-like components 20 is greater than the height of sensor 82 and the height of protrusion 84.

[0088] By doing so, in the case of stacking multiple microflow devices 100C, it is possible to prevent the main surface of the microflow device 100C from contacting the main surface of the plate-shaped component in the adjacent microflow device 100C, thus protecting the main surface of the microflow device.

[0089] Furthermore, ribs can be provided on both sides of the substrate in a single plate-shaped component. Similarly, recesses can also be formed on both sides of the substrate in a single plate-shaped component. By doing so, not only the main surface of the microfluidic device can be protected, but also the other surface.

[0090] Furthermore, when the microfluidic devices 100C are stacked on top of each other, gaps are created between adjacent microfluidic devices 100C. By doing so, the boundaries of each overlapping microfluidic device can be identified by a sensor, thus enabling automatic handling of individual microfluidic devices and automatic determination of the number of stacked microfluidic devices.

[0091] (Variation Example 4) In the housing 101 and incubator 50, the user can extract one microfluidic device from a stacked storage state for use. In Modification 4, an example of the arrangement of ribs and recesses suitable for extracting one microfluidic device from a stacked state will be described.

[0092] Figure 7 This is a diagram used to illustrate the microflow device of variation example 4. Figure 7 The structure of three microflow devices 100D to 100F suitable for extraction is shown in the figure. Figure 7Examples 1-3 correspond to microfluidic devices 100D-100F, respectively. In each microfluidic device, the upper diagram shows the plate-shaped component viewed from above along the positive Z-axis. The middle diagram shows the plate-shaped component viewed from above along the negative Y-axis. The lower diagram shows the plate-shaped component viewed from above along the negative Z-axis.

[0093] In the microfluidic device 100D of Example 1, ribs 31 extending along the Y-axis are arranged on the surface 20DT of the plate-shaped member 20D in the positive Z-axis direction at both ends. As shown in the figure above, when viewed from above in the positive Z-axis direction, the ribs 31 are rectangular with rounded corners.

[0094] As shown in the middle section diagram, rib 31 protrudes in the positive Z-axis direction and extends flatly in the Y-axis direction. Furthermore, as shown in the middle and lower sections, a recess 41 is formed on the surface 20DB of the plate-like member 20D in the negative Z-axis direction, at a position corresponding to rib 31. The length of rib 31 in the X-axis direction is shorter than the length of recess 41 in the X-axis direction. That is, when viewed from above in the Z-axis direction, rib 31 overlaps with recess 41. By doing so, when the plate-like members 20D are stacked in the Z-axis direction, rib 31 is embedded in recess 41.

[0095] As shown in the figure below, the recess 41 is formed on the surface 20DB of the plate-shaped member at both ends along the X-axis direction. The recess 41 is formed as a straight groove extending from one edge of the plate-shaped member 20D along the X-axis direction to the other edge. That is, the recess 41 is formed to extend linearly to the end of the substrate of the plate-shaped member 20D. Furthermore, the end of the recess 41 in the Y-axis direction may not be continuous.

[0096] By forming ribs and recesses in this way, when microfluidic devices 100D are stacked in the Z-axis direction, one microfluidic device 100D can be extracted from the stacked microfluidic devices 100D in the Y-axis direction. Therefore, it is possible to remove one microfluidic device from the stacked microfluidic devices while protecting the main surface of the microfluidic device.

[0097] Furthermore, the end of the rib 31 in the Y-axis direction can also be inclined. By forming the rib 31 in this way, when one microflow device 100D is extracted from the stacked multiple microflow devices 100D, there is no jamming, and it can be extracted more smoothly.

[0098] Furthermore, when the microfluidic devices 100D are stacked on top of each other, gaps are created between adjacent microfluidic devices 100D. By doing so, the boundaries of each overlapping microfluidic device can be identified by a sensor, thus enabling automatic handling of individual microfluidic devices and automatic determination of the number of stacked microfluidic devices.

[0099] In the microfluidic device 100E of Example 2, ribs 33 are arranged near the four corners of the plate-shaped member 20E on the surface 20ET in the positive Z-axis direction. As shown in the figure above, the ribs 33 are circular when viewed from above in the positive Z-axis direction. Furthermore, the ribs 33 can also be triangular, quadrilateral, or other polygonal shapes.

[0100] As shown in the middle section diagram, rib 33 protrudes in the positive Z-axis direction, and a recess 43 is formed on the surface 20EB of the plate-shaped member 20E in the negative Z-axis direction, at a position corresponding to the back of rib 33. Furthermore, as shown in the middle and lower sections, a recess 43 is formed on the surface 20EB of the plate-shaped member 20E in the negative Z-axis direction, at a position corresponding to rib 33. The length of rib 33 in the X-axis direction is smaller than the length of recess 43 in the X-axis direction. That is, when viewed from above in the Z-axis direction, rib 33 overlaps with recess 43. By doing so, when the plate-shaped members 20E are stacked in the Z-axis direction, rib 33 is embedded in recess 43.

[0101] As shown in the figure below, the recess 43 is formed on the surface 20EB of the plate-shaped member at both ends along the X-axis direction. The recess 43 is formed as a straight groove extending from one edge of the plate-shaped member 20E along the X-axis direction to the other edge. That is, the recess 43 is formed to extend linearly to the end of the substrate of the plate-shaped member 20E. Furthermore, the end of the recess 43 in the Y-axis direction may not be continuous.

[0102] By forming ribs and recesses in this way, when microfluidic devices 100E are stacked in the Z-axis direction, one microfluidic device 100E can be extracted from the stacked microfluidic devices 100E in the Y-axis direction. Therefore, it is possible to remove one microfluidic device from the stacked microfluidic devices while protecting the main surface of the microfluidic device.

[0103] Furthermore, the end of the rib 33 in the Y-axis direction can also be inclined. By forming the rib 33 in this way, when one microflow device 100E is extracted from the stacked multiple microflow devices 100E, there is no jamming, and it can be extracted more smoothly.

[0104] Furthermore, when the microfluidic devices 100E are stacked on top of each other, gaps are created between adjacent microfluidic devices 100E. By doing so, the boundaries of each overlapping microfluidic device can be identified by a sensor, thus enabling automatic handling of individual microfluidic devices and automatic determination of the number of stacked microfluidic devices.

[0105] In the microfluidic device 100F of Example 3, ribs 35 extending along the Y-axis are arranged on the surface 20FT of the plate-shaped member 20F in the positive Z-axis direction at both ends along the X-axis direction. As shown in the figure above, when viewed from above in the positive Z-axis direction, the ribs 35 are rectangular with rounded corners.

[0106] As shown in the middle section diagram, rib 35 protrudes in the positive Z-axis direction and extends flatly in the Y-axis direction. Furthermore, as shown in the middle and lower sections, a recess 45 is formed on the surface 20FB of the plate-like member 20F in the negative Z-axis direction, at a position corresponding to rib 35. The length of rib 35 in the X-axis direction is shorter than the length of recess 45 in the X-axis direction. That is, when viewed from above in the Z-axis direction, rib 35 overlaps with recess 45. By doing so, when the plate-like members 20F are stacked in the Z-axis direction, rib 35 is embedded in recess 45.

[0107] As shown in the figure below, a recess 45 is formed on the surface 20FB of the plate-shaped member at both ends along the X-axis direction of the plate-shaped member 20F. The recess 45 is formed as a straight groove extending from one edge of the plate-shaped member 20F along the X-axis direction to the other edge. That is, the recess 45 is formed to extend linearly to the end of the substrate of the plate-shaped member 20F. Furthermore, the end of the recess 45 in the negative Y-axis direction does not penetrate through.

[0108] By forming ribs and recesses in this way, when microfluidic devices 100F are stacked in the Z-axis direction, one microfluidic device 100F can be extracted from the stacked microfluidic devices 100F in the Y-axis direction. Therefore, it is possible to remove one microfluidic device from the stacked microfluidic devices while protecting the main surface of the microfluidic device.

[0109] Furthermore, the end of the rib 35 in the Y-axis direction can also be inclined. By forming the rib 35 in this way, when one microfluidic device 100F is extracted from the stacked multiple microfluidic devices 100F, there is no jamming, and it can be extracted more smoothly.

[0110] Furthermore, when the microfluidic devices 100F are stacked on top of each other, gaps are created between adjacent microfluidic devices 100F. By doing so, the boundaries of each overlapping microfluidic device can be identified by a sensor, thus enabling automatic handling of individual microfluidic devices and automatic determination of the number of stacked microfluidic devices.

[0111] (Variation Example 5) In the housing 101 and incubator 50, the user extracts one microfluidic device from a stacked storage state for use. In Modification 5, an example of the rib and recess configuration suitable for removing the topmost microfluidic device from a state where microfluidic devices are fixed and stacked will be described.

[0112] Figure 8 This is a diagram used to illustrate the microflow device of variation example 5. Figure 8 The structure of three microflow circuit devices 100G to 100I suitable for fixed use is shown in the figure. Figure 8 Examples 1 to 3 correspond to microfluidic devices 100G to 100I, respectively. In each microfluidic device, the upper diagram shows the plate-shaped component viewed from above along the positive Z-axis. The middle diagram shows the plate-shaped component viewed from above along the negative Y-axis. The lower diagram shows the plate-shaped component viewed from above along the negative Z-axis.

[0113] In the microfluidic device 100G of Example 1, a rib 37 is disposed around the end of the plate-shaped member 20G on the surface 20DT in the positive Z-axis direction. That is, the rib 37 is disposed around one surface of the substrate of the plate-shaped member 20G. As shown in the figure above, when viewed from above in the positive Z-axis direction, the rib 37 is a rectangular frame with rounded corners.

[0114] As shown in the middle section diagram, rib 37 protrudes in the positive Z-axis direction and extends flatly in the X and Y-axis directions. Furthermore, as shown in the middle and lower sections, a recess 47 is formed on the surface 20GB of the plate-like member 20G in the negative Z-axis direction, corresponding to rib 37. The X-axis length of the portion of rib 37 extending in the Y-axis direction is shorter than the X-axis length of the portion of recess 47 extending in the Y-axis direction. Furthermore, the Y-axis length of the portion of rib 37 extending in the X-axis direction is shorter than the Y-axis length of the portion of recess 47 extending in the X-axis direction. That is, when viewed from above in the Z-axis direction, rib 37 overlaps with recess 47. By doing so, when the plate-like members 20G are stacked in the Z-axis direction, rib 37 is embedded in recess 47.

[0115] As shown in the figure below, the recess 47 is formed around the edge of the plate-shaped member 20G. That is, the recess 47 is formed around the surface of the substrate of the plate-shaped member 20G where the ribs 37 are not disposed.

[0116] By forming ribs and recesses in this way, the microfluidic device 100G can be fixed and stacked in the Z-axis direction, and the topmost microfluidic device 100G can be removed. Therefore, while protecting the main surface of the microfluidic device, one microfluidic device can be removed from multiple fixed and stacked microfluidic devices.

[0117] Furthermore, when the microfluidic devices 100G are stacked on top of each other, gaps are created between adjacent microfluidic devices 100G. By doing so, the boundaries of each overlapping microfluidic device can be identified by sensors, thus enabling automatic handling of individual microfluidic devices and automatic determination of the number of stacked microfluidic devices.

[0118] In the microfluidic device 100H of Example 2, four ribs 39 extending along the edge of the end of the plate-shaped member 20H are disposed on the surface 20HT of the plate-shaped member 20H in the positive Z-axis direction. As shown in the figure above, the ribs 39 are rectangular when viewed from above in the positive Z-axis direction. Furthermore, the ribs 39 can be triangular, circular, rectangular with rounded corners, or other arbitrary shapes.

[0119] As shown in the middle section diagram, rib 39 protrudes in the positive Z-axis direction and extends flatly in the Y-axis direction. Furthermore, as shown in the middle and lower sections, a recess 49 is formed on the surface 20HB of the plate-like member 20H in the negative Z-axis direction, at a position corresponding to rib 39. The length of each rib 39 in the X-axis direction is shorter than the length of each recess 49 corresponding to each rib 39 in the X-axis direction. Furthermore, the length of each rib 39 in the Y-axis direction is shorter than the length of each recess 49 corresponding to each rib 39 in the Y-axis direction. That is, when viewed from above in the Z-axis direction, the rib 39 overlaps with the recess 49. By doing so, when the plate-like members 20H are stacked in the Z-axis direction, the rib 39 is embedded in the recess 49.

[0120] As shown in the figure below, four recesses 49 are formed in a direction parallel to the edge of the end of the plate-shaped member 20H. That is, the recesses 49 are formed at a position other than the end of the substrate of the plate-shaped member 20H.

[0121] By forming ribs and recesses in this way, the microfluidic device 100H can be fixed and stacked in the Z-axis direction, and the topmost microfluidic device 100H can be removed. Therefore, while protecting the main surface of the microfluidic device, one microfluidic device can be removed from multiple fixed and stacked microfluidic devices.

[0122] Furthermore, when the microfluidic devices 100H are stacked on top of each other, gaps are created between adjacent microfluidic devices 100H. By doing so, the boundary of each overlapping microfluidic device can be identified by a sensor, thus enabling automatic handling of individual microfluidic devices and automatic determination of the number of stacked microfluidic devices.

[0123] In the microfluidic device 100I of Example 3, ribs 30 extending in the Y-axis direction are disposed at both ends of the surface 20IT of the plate-shaped member 20I in the positive Z-axis direction. That is, when the substrate of the plate-shaped member 20I is generally rectangular, the ribs 30 include at least two ribs: ribs disposed in contact with the X-axis direction edge of one surface of the plate-shaped member 20I in the positive Y-axis direction; and ribs disposed in contact with the other edges of one surface of the plate-shaped member 20I. As shown in the figure above, when viewed from the positive Z-axis direction, the ribs 30 are quadrilaterals. Furthermore, the ribs 30 can be triangular, quadrilateral, rectangular with rounded corners, or other arbitrary shapes.

[0124] As shown in the middle section diagram, rib 30 protrudes in the positive Z-axis direction and extends flatly in the Y-axis direction. Furthermore, as shown in the middle and lower sections, a recess 40 is formed on the surface 20IB of the plate-like member 20I in the negative Z-axis direction, at a position corresponding to rib 30. The length of rib 30 in the X-axis direction is shorter than the length of recess 40 in the X-axis direction. That is, when viewed from above in the Z-axis direction, rib 30 overlaps with recess 40. By doing so, when the plate-like members 20I are stacked in the Z-axis direction, rib 30 is embedded in recess 40.

[0125] As shown in the figure below, the recess 40 is formed on the surface 20IB of the plate-shaped member in a direction parallel to the edges at both ends of the plate-shaped member 20I in the X-axis direction. The recess 40 is formed to extend through one edge or the other edge of the plate-shaped member 20I in the X-axis direction. Furthermore, multiple recesses 40 need to be formed on both edges.

[0126] By forming ribs and recesses in this way, the microfluidic device 100I can be fixed and stacked in the Z-axis direction, and the topmost microfluidic device 100I can be removed. Therefore, while protecting the main surface of the microfluidic device, one microfluidic device can be removed from multiple fixed and stacked microfluidic devices.

[0127] Furthermore, when the microfluidic devices 100I are stacked on top of each other, gaps are created between adjacent microfluidic devices 100I. By doing so, the boundary of each overlapping microfluidic device can be identified by a sensor, thus enabling automatic handling of individual microfluidic devices and automatic determination of the number of stacked microfluidic devices.

[0128] (Variation Example 6) exist Figure 7 , Figure 8 In Example 6, the structure of the ribs and recesses when the microflow device is stacked is described. In Modification 6, the structure of the ribs and recesses when the microflow device is configured in another device is described.

[0129] Figure 9 This is a diagram illustrating the detailed structure of the housing 101, which is an example of other devices. (See reference...) Figure 9 The receiving section 101 includes a frame 4 and a container 1. The container 1 is loaded onto the frame 4. The container 1 is detachable from the frame 4. The container 1 includes a handle 2 and a body 3. The user holds the handle 2 to load the container 1 onto the frame 4 and to remove the container 1 from the frame 4.

[0130] like Figure 9 As shown, space 3X represents the internal space of the main body 3 of container 1. Guide members 3A are arranged at each corner inside the main body 3. An opening 3Y is provided on the front surface of the main body 3. Figure 9 The diagram shows the state in which the microflow device 100 is housed inside the body 3. The bottom surface 3Z shows the bottom surface of the body 3 of the container 1.

[0131] New microfluidic devices 100, ready for use, are successively placed on the bottom surface 3Z or on microfluidic devices already stacked on the bottom surface 3Z. When removing the microfluidic devices 100 housed in the housing section 101, they are sequentially extracted in the direction of arrow AR1, starting from the bottommost microfluidic device 100 in the stacked microfluidic devices 100. The conveying section 500 extracts the microfluidic devices 100 that are in contact with the bottom surface 3Z from among the multiple microfluidic devices 100 stacked inside the main body 3 via the opening 3Y.

[0132] Figure 10 It is used for explanation Figure 9 A diagram showing the structure of the bottom surface 3Z of the housing section 101. Figure 10 In the image, (a) shows the microflow device 1000A, (b) shows the bottom surface 3Z of the receiving portion 101, and (c) shows the rib 310 of the microflow device 1000A embedded in the recess 410 of the receiving portion 101.

[0133] The plate-shaped component 200A of the microfluidic device 1000A is equipped with a... Figure 7 Similar to Example 1, a rib 310 extends along the short side of the plate-shaped member (upper section (a)). At this time, a recess 410 corresponding to the rib 310 is formed on the bottom surface 3Z of the container 1 of the receiving part 101 (middle section (b)). With such a structure, as shown in the figure of the lower section (c), the rib 310 of the plate-shaped member 200A of the microfluidic device 1000A is embedded in the recess 410 on the bottom surface 3Z of the container 1 of the receiving part 101.

[0134] In addition, the recess 410 is in Figure 9 The opening extends through the 3Y direction. This allows the microflow device located at the bottom to be extracted in the direction of arrow AR1.

[0135] Therefore, it is possible to extract one microflow device from multiple stacked microflow devices while protecting the main surface of the microflow device.

[0136] Figure 11 It is used for explanation Figure 9 A diagram showing the structure of the bottom surface 5Z of the containment section 101. Figure 11 In the image, (a) shows the microflow device 1000B, (b) shows the bottom surface 5Z of the receiving portion 101, and (c) shows the rib 330 of the microflow device 1000B embedded in the recess 430 of the receiving portion 101.

[0137] The plate-shaped component 200B of the microfluidic device 1000B is equipped with a... Figure 8 Similar to Example 3, ribs 330 exist at the four corners of the plate-shaped component (upper section (a)). At this time, a recess 430 corresponding to the ribs 330 is formed on the bottom surface 5Z of the receiving portion 101 (middle section (b)). With such a structure, as shown in the figure of the lower section (c), the ribs 330 of the plate-shaped component 200B of the microflow path device 1000B are embedded in the recess 430 on the bottom surface 5Z of the receiving portion 101.

[0138] Therefore, it is possible to remove one microflow device from multiple fixed and stacked microflow devices while protecting the main surface of the microflow device from damage to the main surfaces of other microflow devices.

[0139] Thus, when a microfluidic device is configured in other devices, the structure of the ribs of the microfluidic device and the recesses of the other devices allows one microfluidic device to be removed from multiple stacked microfluidic devices while protecting the main surface of the microfluidic device from damage to the main surface of other microfluidic devices.

[0140] [Way] Those skilled in the art will understand that the above-described exemplary embodiments are specific examples of the following approaches.

[0141] (Item 1) A microfluidic device according to one embodiment includes: a plate-shaped member; a protrusion disposed on the plate-shaped member; and a first protrusion disposed on the plate-shaped member. The plate-shaped member has a substrate having a first surface and a second surface, and a plurality of microfluidic paths are formed on one of the surfaces. The protrusion and the first protrusion are disposed on the first surface of the substrate. The distance from the first surface to the farthest end of the first protrusion is greater than the distance from the first surface to the farthest end of the protrusion.

[0142] According to the microfluidic device of item 1, ribs are arranged on the plate-shaped component of the microfluidic device. Therefore, when the microfluidic devices are stacked and stored, the main surface of the microfluidic device can be prevented from contacting other microfluidic devices, thus protecting the main surface of the microfluidic device.

[0143] (Item 2) In the microfluidic device described in Item 1, a first recess is formed on the second surface of the substrate. When the plate-shaped component is viewed from the normal direction of the first surface, the first protrusion overlaps with the first recess.

[0144] According to the microfluidic device in item 2, when viewed from above, the recess is formed to include a rib. Thus, when stacking microfluidic devices, the ribs are embedded in the recesses of other microfluidic devices, thereby enabling the microfluidic devices to be fixed or removed using the shapes of the ribs and recesses while protecting the main surface of the microfluidic device.

[0145] (Item 3) In the microflow device described in Item 2, the first recess is formed at a location other than the end of the substrate.

[0146] According to the microfluidic device of item 3, the recess is formed so as not to be in contact with the end of the substrate, and the rib is formed to be embedded in the recess. Thus, the microfluidic device can be fixed while protecting its main surface.

[0147] (Item 4) The microfluidic device described in Item 2 further includes a second protrusion disposed on a first surface of a substrate. A second recess is formed on a second surface of the substrate. The substrate is generally rectangular. The first protrusion is disposed in contact with a first edge of the first surface of the substrate. The second protrusion is disposed at a different position on the first surface of the substrate, in contact with a second, third, or fourth edge. When viewed from above, the second protrusion overlaps with the second recess.

[0148] According to the microfluidic device of item 4, two sets of ribs and recesses are formed in a manner that connects to the edge of a rectangular substrate, and the edges of each set of connections are different. Therefore, since the microfluidic device cannot be removed, it is possible to fix the microfluidic device while protecting its main surface.

[0149] (Item 5) In the microfluidic device described in Item 2, a first recess is formed around the second surface of the substrate. A first protrusion is disposed around the first surface of the substrate.

[0150] According to the microfluidic device of item 5, a recess is formed around one surface of the substrate, and a rib is formed around the other surface of the substrate. This allows the microfluidic device to be fixed while protecting its main surface.

[0151] (Item 6) In the microflow device described in Item 2, the first recess is formed to extend linearly to the end of the substrate.

[0152] According to the microfluidic device of item 6, the recess is formed to be in contact with the edge of the substrate surface. Therefore, the microfluidic device can be extracted while protecting its main surface.

[0153] (Item 7) In the microfluidic device described in Item 6, the protrusion extends in a first direction and is inclined at the end of the protrusion in the first direction.

[0154] According to the microfluidic device of item 7, the ends of the ribs on the substrate are inclined. Thus, when a microfluidic device is removed from a stack of multiple microfluidic devices, the main surface of the microfluidic device can be protected from corner damage by the ribs of the microfluidic devices above or below.

[0155] (Item 8) The microfluidic device according to any one of items 1 to 7 further comprises a functional component disposed on a substrate. In the normal direction of the plate-shaped component, the distance from the first surface to the farthest end of the first protrusion is greater than the distance from the surface on which the functional component is disposed to the farthest end of the functional component.

[0156] According to item 8, in microfluidic devices, the height of the ribs is higher than the height of the functional components. Therefore, when a microfluidic device is stacked on a table or a similarly structured microfluidic device, it is not the functional components but the ribs that contact the support platform or the similarly structured microfluidic device. Thus, the observed area and functional components such as sensors on the main surface of the microfluidic device do not contact the support platform or the similarly structured microfluidic device, protecting the main surface of the microfluidic device.

[0157] (Item 9) The microfluidic device according to any one of items 2 to 7 further comprises a functional component disposed on a substrate. In the normal direction of the plate-shaped component, the value obtained by subtracting the distance from the second surface to the bottom of the first recess from the distance from the first surface to the farthest end of the first protrusion is greater than the distance from the surface on which the functional component is disposed to the farthest end of the functional component.

[0158] According to item 9, the value of subtracting the depth of the recess from the height of the rib is greater than the height of the functional component. Therefore, when stacking microfluidic devices with the same structure, it is not the functional component but the rib that contacts the microfluidic device above or below. Thus, the observed area on the main surface of the microfluidic device and functional components such as sensors do not contact the microfluidic device with the same structure, protecting the main surface of the microfluidic device.

[0159] (Item 10) The microflow device according to any one of items 1 to 9 further comprises a third protrusion disposed on the second surface of the substrate.

[0160] According to the microfluidic device of item 10, ribs are disposed on both one surface and the other surface of the substrate. Therefore, when the microfluidic device is stacked on a support stage or a similar microfluidic device, the ribs contact the support stage or the similar microfluidic device. Thus, the main surface and other surfaces of the microfluidic device do not contact the support stage or the similar microfluidic device, thereby protecting the main surface and other surfaces of the microfluidic device.

[0161] (Item 11) In the microfluidic device described in Item 10, a third recess is formed on the first surface of the substrate, and when the plate-shaped component is viewed from above, the third protrusion overlaps with the third recess.

[0162] According to the microfluidic device of claim 11, ribs are disposed on both one and another surface of the substrate, and a recess corresponding to the ribs on the other surface is formed on one surface. Therefore, when the microfluidic device is stacked on a support stage or a similar microfluidic device, the ribs contact the support stage or the similar microfluidic device. Thus, the main surface and other surfaces of the microfluidic device do not contact the support stage or the similar microfluidic device, protecting the main surface and other surfaces of the microfluidic device.

[0163] (Item 12) In any one of items 1 to 11, in the plate-shaped component, the surface of the substrate on which multiple microchannels are formed further has a thin film covering the multiple microchannels. The distance from the first surface to the farthest end of the first protrusion is greater than the distance from the surface of the substrate on which multiple microchannels are formed to the farthest end of the thin film.

[0164] According to the microfluidic device in item 12, the height of the rib is greater than the thickness of the film covering the microfluidic path. Therefore, when stacking microfluidic devices with the same structure, it is the rib, not the film, that contacts the microfluidic device above or below. Consequently, the main surface of the microfluidic device does not contact other microfluidic devices with the same structure, thus protecting the main surface of the microfluidic device.

[0165] (Item 13) In the microflow device described in Item 8 or 9, the functional component includes RFID (Radio Frequency Identification) that stores identification information. The identification information is used for determining the number of chips in the microflow device.

[0166] (Item 14) In any one of items 1 to 13, the protrusion is an opening for injecting a reagent into the microfluidic device.

[0167] The embodiments disclosed herein should be considered illustrative rather than restrictive in all respects. The scope of the invention is defined not by the description of the above embodiments but by the claims, and is intended to include all modifications within the meaning and scope equivalent to the claims.

[0168] Explanation of reference numerals in the attached figures 1 Container 2 handles 3. Main Body 3A Guide 3X Space 3Y, 22, 26, 29 Openings 3Z bottom surface 4. Frame 5Z Bottom 18 substrate 19 films 50 Incubator Plate-shaped components: 20, 20A, 20B, 20C, 20D, 20E, 20F, 20G, 20H, 20I, 200A, 200B 23 Mainstream Roads 24 Microflow path 25 Storage Department 27 Gas permeable membrane 28 Recycling Department 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 310, 330 Ribs 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 430 recess 82 Sensors 84. Protrusions 100, 100A, 100B, 100C, 1000A, 1000B Microflow Devices 101 Containment Department 103 Injection Section 120 Control Department 122 processor 140 Microscope Camera 142 Objective lens 144. Focus Change Agency 146 Image Sensor 160 Platform 162 Camera Field of View Changing Mechanism 162X X-axis moving mechanism 162Y Y-axis moving mechanism 164 lighting fixtures 180 Reading Department 190 Abandoned Department 200 Test apparatus 500 Moving Department.

Claims

1. A microfluidic device comprising: a plate-shaped component; Protrusions disposed on the plate-like component; and The first protrusion disposed on the plate-shaped component The plate-shaped component has a substrate, the substrate having a first surface and a second surface, and multiple microflow paths formed on one of the surfaces. The protrusion and the first protrusion are disposed on the first surface of the substrate. The distance from the first surface to the farthest end of the first protrusion is greater than the distance from the first surface to the farthest end of the protrusion.

2. The microfluidic device as described in claim 1, wherein, A first recess is formed on the second surface of the substrate. When the plate-shaped component is viewed from above in the direction of the normal to the first surface, the first protrusion overlaps with the first recess.

3. The microfluidic device as described in claim 2, wherein, The first recess is formed at a location other than the end of the substrate.

4. The microfluidic device as described in claim 2, wherein, It also includes a second protrusion disposed on the first surface of the substrate. A second recess is formed on the second surface of the substrate. The substrate is roughly rectangular in shape. The first protrusion is disposed in contact with a first edge of the first surface of the substrate. The second protrusion is disposed at a position different from the first protrusion on the first surface of the substrate, and is in contact with a second side, a third side, or a fourth side. When viewed from above, the second protrusion overlaps with the second recess.

5. The microfluidic device as described in claim 2, wherein, The first recess is formed around the second surface of the substrate. The first protrusion is disposed around the first surface of the substrate.

6. The microfluidic device as described in claim 2, wherein, The first recess is formed in a straight line extending to the end of the substrate.

7. The microfluidic device as described in claim 6, wherein, The first protrusion extends in a first direction. An inclination is formed at the end of the first protrusion in the first direction.

8. The microfluidic device as claimed in claim 1, wherein, It also includes functional components disposed on the substrate. In the normal direction of the plate-shaped member, the distance from the first surface to the farthest end of the first protrusion is greater than the distance from the surface on which the functional member is provided to the farthest end of the functional member.

9. The microfluidic device as described in claim 2, wherein, It also includes functional components disposed on the substrate. In the normal direction of the plate-shaped component, the value obtained by subtracting the distance from the second surface to the bottom of the first recess from the distance from the first surface to the farthest end of the first protrusion is greater than the distance from the surface on which the functional component is provided to the farthest end of the functional component.

10. The microflow device according to any one of claims 1 to 9, wherein, It also includes a third protrusion disposed on the second surface of the substrate.

11. The microfluidic device of claim 10, wherein, A third recess is formed on the first surface of the substrate. When viewed from above, the third protrusion overlaps with the third recess.

12. The microfluidic device of claim 11, wherein, The plate-shaped component, on the surface of the substrate where the plurality of microflow paths are formed, also has a thin film covering the plurality of microflow paths. The distance from the first surface to the farthest end of the first protrusion is greater than the distance from the surface of the substrate on which the plurality of microflow paths are formed to the farthest end of the thin film.

13. The microfluidic device as claimed in claim 8 or 9, wherein, The functional component includes RFID (Radio Frequency Identification) which stores identification information. The identification information is used to determine the number of microflow devices.

14. The microfluidic device as claimed in claim 1, wherein, The protrusion is an opening for injecting reagents into the microfluidic device.

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