Poria and Ganoderma spore powder feed for improving disease resistance in chickens and its preparation method

Abstract

This invention discloses a Poria cocos and Ganoderma lucidum spore powder feed for improving the disease resistance of chicken flocks and its preparation method, relating to the field of functional livestock and poultry feed technology. The feed includes: a basal feed and host-response triggered functional particles; the host-response triggered functional particles include a composite active core and a multi-factor coupling response layer coating the outside of the composite active core; the composite active core includes active components derived from Poria cocos and active components derived from Ganoderma lucidum spore powder; the active components derived from Poria cocos are Poria cocos polysaccharides and / or Poria cocos polysaccharide degradation fragments; the active components derived from Ganoderma lucidum spore powder are broken-cell wall Ganoderma lucidum spore powder, Ganoderma lucidum spore powder extract, and / or Ganoderma lucidum spore powder conversion products. This invention improves the accuracy of the timing of activity presentation and the specificity of the action site, promotes the effective contact, recognition, and utilization of active components in the intestinal tract, and helps maintain the stability and controllability of functional components.

Description

Technical Field

[0001] This invention relates to the field of functional livestock and poultry feed technology, and in particular to a feed made from Poria cocos and Ganoderma lucidum spores for improving the disease resistance of chicken flocks, and its preparation method. Background Technology

[0002] In recent years, functional livestock and poultry feeds have gradually shifted from simple nutritional supplementation to precise regulation of host health. Research on active substances derived from traditional Chinese medicine and edible and medicinal fungi as feed functional factors is increasing. Among them, Poria cocos polysaccharides, Ganoderma lucidum spore powder and its extracts, due to their potential for immune regulation, intestinal homeostasis maintenance, and stress-induced sustained release, have been applied to the development of products related to enhancing disease resistance in chicken flocks. Simultaneously, delivery technologies such as microencapsulation, encapsulation, and controlled-release are beginning to be introduced into feed formulation design to improve the stability and utilization of active components during processing, storage, and digestion.

[0003] While existing technologies can add and protect active ingredients from Poria cocos or Ganoderma lucidum, most still rely on static encapsulation or ordinary sustained-release modes. The release of active ingredients depends more on time, single gastrointestinal conditions, or passive disintegration. They lack selective triggering mechanisms for the pathological microenvironment of infection or inflammation in chickens, making it difficult to balance stable shielding in healthy states with targeted exposure in pathological states. This results in insufficient precision in the timing and site of action of the active interface, thereby limiting the effective utilization and disease-modulating potential of functional components in intestinal pathological scenarios. Summary of the Invention

[0004] In view of this, this application provides a feed containing Poria cocos and Ganoderma lucidum spores for improving the disease resistance of chicken flocks and a method for its preparation.

[0005] According to one aspect of this disclosure, a Poria cocos and Ganoderma lucidum spore powder feed for improving the disease resistance of chicken flocks is provided, comprising: the Poria cocos and Ganoderma lucidum spore powder feed comprising a basic feed and host response triggered functional particles, wherein the host response triggered functional particles comprise a composite active core and a multi-factor coupling response layer coated on the outside of the composite active core; The composite active core includes active components derived from Poria cocos and active components derived from Ganoderma lucidum spore powder. The active components derived from Poria cocos are Poria cocos polysaccharides and / or Poria cocos polysaccharide degradation fragments, and the active components derived from Ganoderma lucidum spore powder are broken-cell wall Ganoderma lucidum spore powder, Ganoderma lucidum spore powder extract and / or Ganoderma lucidum spore powder conversion products. The multi-factor coupling response layer maintains a shielded state against the complex active core in the healthy intestinal environment of chickens, and generates a coupling response to at least two of the factors among the increased reactive oxygen species level, changes in intestinal pH, and changes in intestinal enzyme environment in the intestinal pathological microenvironment related to infection or inflammation in chickens. By generating a coupling response through two factors, the outer layer of host-response-triggered functional particles is deshielded, and the glycosyl interface derived from Poria cocos and / or the active interface derived from Ganoderma lucidum spore powder on the surface of the composite active core are exposed.

[0006] According to one aspect of this disclosure, a method for preparing Poria cocos and Ganoderma lucidum spore powder feed for improving the disease resistance of chicken flocks is provided, comprising: Step 1: extracting and enzymatically hydrolyzing Poria cocos raw material to obtain active components derived from Poria cocos; Step 2: The Ganoderma lucidum spore powder is subjected to cell wall breaking and extraction processes to obtain the active components derived from the Ganoderma lucidum spore powder; Step 3: Combine and pre-assemble the active components from Poria cocos with the active components from Ganoderma lucidum spore powder to form a composite active core; Step 4: A responsive shielding layer is formed on the outside of the composite active core, generating host-response triggered functional particles; Step 5: Mix the host response-triggered functional particles with the basic feed to obtain Poria cocos and Ganoderma lucidum spore powder feed.

[0007] The beneficial effects of this invention are as follows: By constructing a multi-factor coupled response layer outside the composite active core, the host response-triggered functional particles maintain shielding stability in the healthy intestinal environment. However, in the pathological intestinal microenvironment related to infection or inflammation, the outer layer is deshielded and the glycosyl interface derived from Poria cocos and the active interface derived from Ganoderma lucidum spore powder on the surface of the composite active core are exposed through the synergistic triggering of at least two types of signals in the reactive oxygen species level, pH, and enzyme environment. This enables the release behavior of functional particles to be correlated with the host pathological state, improves the timing accuracy and site-specificity of activity presentation, promotes the effective contact, recognition, and utilization of active components in the local intestinal environment, and helps maintain the stability and controllability of the functional components. Attached Figure Description

[0008] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0009] Figure 1 A comparison chart of the structural performance of host-response triggered functional particles.

[0010] Figure 2 This is a graph showing the relationship between the combination of composite active nuclear parameters and the overall disease resistance effect.

[0011] Figure 3 This is a graph showing the relationship between the ability to respond to pathological environments and the final efficacy of disease resistance. Detailed Implementation

[0012] To make the objectives, technical solutions, and advantages of the embodiments of this disclosure clearer, the technical solutions of the embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this disclosure. All other embodiments obtained by those skilled in the art based on the described embodiments of this disclosure without creative effort are within the scope of protection of this disclosure.

[0013] This invention aims to construct a Poria cocos and Ganoderma lucidum spore powder feed for improving the disease resistance of chicken flocks. In the embodiments of this application, the Poria cocos and Ganoderma lucidum spore powder feed includes a basic feed and host response triggered functional particles. The host response triggered functional particles include a composite active core and a multi-factor coupled response layer coating the outside of the composite active core. The composite active core includes active components derived from Poria cocos and active components derived from Ganoderma lucidum spore powder. The active components derived from Poria cocos are Poria cocos polysaccharides and / or Poria cocos polysaccharide degradation fragments, and the active components derived from Ganoderma lucidum spore powder are broken-cell wall Ganoderma lucidum spore powder, Ganoderma lucidum spore powder extract and / or Ganoderma lucidum spore powder conversion products. The multi-factor coupling response layer maintains a shielded state against the complex active core in the healthy intestinal environment of chickens, and generates a coupling response to at least two of the factors among the increased reactive oxygen species level, changes in intestinal pH, and changes in intestinal enzyme environment in the intestinal pathological microenvironment related to infection or inflammation in chickens. It should be noted that a simulation system of a healthy gut environment and a pathological microenvironment was established, and functional particles were placed in them for treatment. The presence of multi-factor coupling response was determined by the integrity of the coating layer, the release rate, or changes in morphology.

[0014] By generating a coupling response through two factors, the outer layer of host-response-triggered functional particles is deshielded, and the glycosyl interface derived from Poria cocos and / or the active interface derived from Ganoderma lucidum spore powder on the surface of the composite active core are exposed.

[0015] It should be noted that the surface composition of the particles before and after treatment should be detected or observed under a microscope, and the changes in the coating state and the enhancement of the nuclear surface characteristic signals should be used as the basis for determining deshielding and interface exposure.

[0016] In some embodiments of this application, the active components derived from Poria cocos and the active components derived from Ganoderma lucidum spore powder are pre-formed into a composite active core, and the mass ratio of the active components derived from Poria cocos to the active components derived from Ganoderma lucidum spore powder in the composite active core is 1:(0.1-5).

[0017] In some embodiments of this application, the number-average molecular weight of the active component derived from Poria cocos is 0.5 kDa to 200 kDa; The cell wall breakage rate of the Ganoderma lucidum spore powder in the active components of the Ganoderma lucidum spore powder is over 70%.

[0018] In some embodiments of this application, the multi-factor coupled response layer includes an outer response shielding layer and an inner response regulation layer; It should be noted that the inner response control layer is formed first, followed by the outer response shielding layer, and the formation of the double-layer structure is confirmed by layer observation or surface inspection.

[0019] The external response shielding layer prevents premature exposure of the active nucleus in a healthy intestinal environment, while the internal response regulation layer undergoes at least one of the following changes in the intestinal pathological microenvironment related to infection or inflammation: degradation, dissociation, swelling, cracking, or rearrangement.

[0020] It should be noted that the bilayer particles were treated in different simulated environments, and the outer layer was tested for detachment, loosening, or increased permeability after the inner layer changed.

[0021] In some embodiments of this application, the multi-factor coupled response layer is any one of the following: reactive oxygen species response coupled with enzyme response, reactive oxygen species response coupled with pH response, and enzyme response coupled with pH response.

[0022] It should be noted that corresponding sensitive primitives are introduced into the response layer, and whether a coupled response is formed is determined by comparing single-factor and two-factor processing.

[0023] In some embodiments of this application, the amount of the host-response triggered functional pellet added to the basal feed is 0.01% to 15%.

[0024] This application embodiment also provides a method for preparing Poria cocos and Ganoderma lucidum spore powder feed for improving the disease resistance of chicken flocks, which includes: Step 1: Extracting and enzymatically hydrolyzing Poria cocos raw materials to obtain active components from Poria cocos; Step 2: The Ganoderma lucidum spore powder is subjected to cell wall breaking and extraction processes to obtain the active components derived from the Ganoderma lucidum spore powder; Step 3: Combine and pre-assemble the active components from Poria cocos with the active components from Ganoderma lucidum spore powder to form a composite active core; Step 4: A responsive shielding layer is formed on the outside of the composite active core, generating host-response triggered functional particles; It should be noted that the composite active core and the shielding layer are coated in contact, and the coating sequence, time and curing conditions are controlled to obtain responsive functional particles.

[0025] Step 5: Mix the host response-triggered functional particles with the basic feed to obtain Poria cocos and Ganoderma lucidum spore powder feed.

[0026] In some embodiments of this application, the formation of the composite active core is achieved by combining active components derived from Poria cocos with active components derived from Ganoderma lucidum spore powder and then assembling them.

[0027] In some embodiments of this application, the responsive shielding layer is formed by mixing the composite active core and the shielding layer forming component and attaching them to the outer surface of the composite active core; the shielding layer forming component attached to the outer surface of the composite active core is then cured or cross-linked. It should be noted that the shielding layer components are fixed on the surface of the composite active core by adjusting the pH, temperature, or adding a crosslinking agent, and their stability and responsiveness are tested after curing.

[0028] The shielding layer forming component is one or more of polysaccharide coating materials, protein coating materials, or lipid coating materials.

[0029] In some embodiments of this application, forming a responsive shielding layer refers to first forming an inner response regulation layer outside the composite active core, and then forming an outer response shielding layer outside the inner response regulation layer.

[0030] It should be noted that the step-by-step coating was completed in the order of inner layer first and then outer layer, and the process of inner layer changing first and outer layer failing later was observed in the pathological microenvironment.

[0031] Example 1: 0.45% host-response triggered functional particles were added to the basic feed. The composite active core of the functional particles consisted of active components derived from Poria cocos and active components derived from Ganoderma lucidum spore powder. The Poria cocos-derived active components were obtained by pulverizing, water-extracting, enzymatically hydrolyzing, concentrating, and drying Poria cocos, with a number-average molecular weight of 12.6 kDa. The Ganoderma lucidum spore powder-derived active components were obtained by extraction, concentration, and drying of Ganoderma lucidum spore powder with a cell wall breakage rate of 91.4%. The two were compounded at a mass ratio of 1:0.75 and adjusted to pH... After stirring and pre-assembling under 6.0 conditions, a composite active core is formed. Outside the composite active core, an internal response regulation layer is first formed with thioketated carboxymethyl chitosan and low-methoxyl pectin, and then an external response shielding layer is formed with zein and sodium alginate. Subsequently, the particles are cured with calcium ions and spray-dried to obtain bilayer multi-factor coupled response functional particles. The obtained samples have good shielding stability in a healthy intestinal simulated environment. In the microenvironment of infection or inflammation-related intestinal pathology, significant deshielding and active core exposure can occur. After challenge, the morbidity rate and intestinal bacterial load of chicken flocks can be significantly reduced.

[0032] Example 2: 0.62% of host-response triggered functional particles were added to the basic feed. The composite active core of the functional particles consisted of active components derived from Poria cocos with a number average molecular weight of 18.4 kDa and active components derived from Ganoderma lucidum spore powder with a cell wall breakage rate of 93.1%. The two were added to purified water at a mass ratio of 1:0.90, stirred at pH 5.8, and dispersed under high shear to form the composite active core. Subsequently, an internal response regulation layer composed of thioketalized carboxymethyl chitosan and sodium alginate was first formed outside the composite active core, followed by an external response shielding layer composed of zein and gelatin. The encapsulation was completed by enzymatic solidification, resulting in functional particles with reactive oxygen species response and pH response coupling characteristics. The obtained sample showed low premature release in the healthy simulation system and increased release in the pathological simulation system, indicating that it could more fully expose the composite active core under pathological conditions and showed good immune enhancement and anti-infection effects after chicken flock challenge.

[0033] Example 3: 0.38% of host-response-triggered functional particles were added to the basic feed. The composite active core in the functional particles was formed by mixing active components derived from Poria cocos with a number-average molecular weight of 8.9 kDa and active components derived from Ganoderma lucidum spore powder with a cell wall breakage rate of 88.6% at a mass ratio of 1:0.75. After mixing, the particles were pre-assembled at 45°C for 30 min. The outer layer adopted a double-layer coating method, with the inner response regulation layer formed by low-methoxyl pectin and sodium alginate, and the outer response shielding layer formed by zein. The particles were then solidified with calcium chloride to obtain the functional particles. This sample constructed an enzyme response and pH response coupled structure, which could maintain relatively complete coating under healthy conditions. Under infection-related intestinal pathological conditions, changes in the inner layer and increased permeability of the outer layer were observed, thereby promoting the release of the composite active core and showing a certain disease resistance promotion effect. However, the overall response intensity and the final disease resistance effect were lower than those of the more preferred dual-factor high-response implementation method.

[0034] Example 4: 0.73% of host-response-triggered functional particles were added to the basic feed. The composite active core of the functional particles consisted of an active component derived from Poria cocos with a number-average molecular weight of 24.7 kDa and an active component derived from Ganoderma lucidum spore powder with a cell wall breakage rate of 94.2%. The two were mixed at a mass ratio of 1:1.30 and stirred and homogenized at pH 5.9 to form a composite active core. Subsequently, an inner response regulation layer composed of thioketalized carboxymethyl chitosan and pectin was first coated on the outside of the composite active core, and then an outer response shielding layer composed of zein and chitosan was coated on the outside. The core was then fixed by calcium ion cross-linking to obtain a double-layer multi-factor coupled response functional particle. The obtained sample had sufficient deshielding ability and obvious core surface exposure characteristics in the pathological microenvironment, and could more effectively release the glycosyl interface derived from Poria cocos and the active interface derived from Ganoderma lucidum. Therefore, after chickens were challenged with the virus, they showed a lower morbidity and a lower bacterial load, and the overall disease resistance effect was better.

[0035] Example 5: 0.58% host-response triggered functional particles were added to the basic feed. The composite active core of the functional particles consisted of an active component derived from Poria cocos with a number-average molecular weight of 15.8 kDa and an active component derived from Ganoderma lucidum spore powder with a cell wall breakage rate of 95.0%. These two components were mixed at a mass ratio of 1:1.80 and then subjected to a two-stage homogenization pre-assembly to improve the interfacial binding between the two types of active components. Subsequently, a response layer was constructed in the order of inner layer first, then outer layer. The inner response regulation layer consisted of thioketated carboxymethyl chitosan and low-methoxyl pectin, while the outer response shielding layer consisted of zein and sodium alginate. The host-response triggered functional particles were obtained through pH adjustment, cross-linking curing, and drying. The obtained sample showed good shielding protection for the composite active core in a healthy intestinal environment. In infection or inflammation-related pathological microenvironments, it exhibited the most obvious two-factor coupling response, deshielding, and active core exposure effects, thus showing the best overall disease resistance effect. It could reduce the incidence rate and cecal bacterial load after challenge and increase serum IgA levels. Figure 3 As shown, the response intensity, deshielding degree, and sufficiency of active nucleus exposure of host response-triggered functional particles in the pathological microenvironment related to infection or inflammation are well consistent with their ultimate effects of reducing morbidity, reducing bacterial load, and improving immune indicators. Among them, the more sufficiency of the pathological environment response in the implementation method, the better its overall anti-disease final effect is usually, indicating that the technical route of the present invention to enhance disease resistance through pathological state-triggered release has inherent rationality.

[0036] Example 6: 0.81% host-response triggered functional particles were added to the basic feed. The composite active core of the functional particles was formed by combining active components derived from Poria cocos with a number average molecular weight of 31.5 kDa and active components derived from Ganoderma lucidum spore powder with a cell wall breakage rate of 90.7% at a mass ratio of 1:0.70. An internal response regulation layer composed of thioketalized carboxymethyl chitosan and sodium alginate was first formed outside the composite active core, followed by an external response shielding layer composed of zein and pectin. The coating layer was fixed by thermosetting and calcium ion cross-linking. The resulting functional particles were then added to the basic feed. The obtained sample also showed a significant response and promoted the release of the active core under pathological conditions, exhibiting a relatively stable disease resistance enhancement effect. However, due to the relatively high molecular weight of the active components derived from Poria cocos and the relatively Poria cocos / Ganoderma lucidum ratio favoring Poria cocos, the interface exposure sufficiency under pathological conditions was slightly inferior to the optimal embodiment. Figure 2As shown, the number-average molecular weight of the active components derived from Poria cocos in the composite active core and the mass ratio between the active components derived from Poria cocos and the active components derived from Ganoderma lucidum spore powder have a significant impact on the sufficiency of interface exposure of host response-triggered functional particles in the pathological environment and the final comprehensive anti-disease effect. Among them, when the relevant parameter combination is within a better range, the resulting functional particles are more likely to achieve a full response and release of activity under pathological conditions, thus exhibiting a better anti-disease promotion effect.

[0037] Comparative Example 1: 0.58% of a mixed powder of active ingredients was added to the basic feed. The mixed powder was prepared by spray drying after direct mixing of the active ingredients derived from Poria cocos with a number average molecular weight of 15.8 kDa and the active ingredients derived from Ganoderma lucidum spore powder with a cell wall breakage rate of 95.0% at a mass ratio of 1:1.80. No multi-factor coupling response layer of host response triggering functional particles was set, nor was a double-layer shielding structure constructed. Although the obtained sample contained two types of active ingredients, Poria cocos and Ganoderma lucidum spore powder, due to the lack of shielding protection under healthy conditions and triggering release mechanism under pathological conditions, a high premature release occurred in the healthy simulated environment, making it difficult to achieve targeted exposure and enhanced release under pathological conditions. Therefore, its disease resistance performance after challenge was significantly weaker than that of the other examples.

[0038] Comparative Example 2: 0.58% of coated particles were added to the basal feed. The composite active core in the particles was prepared by a mass ratio of 1:1.80 of active components derived from Poria cocos with a number average molecular weight of 15.8 kDa and active components derived from Ganoderma lucidum spore powder with a cell wall breakage rate of 95.0%. Only a single layer of sodium alginate coating was set on the outside, without forming a double-layer structure consisting of an internal response regulation layer and an external response shielding layer, and without introducing reactive oxygen response units. The preparation process was carried out by simple mixing, coating and calcium ion solidification to obtain single-layer coated particles. Although the obtained sample could partially release under certain conditions, its recognition of the pathological microenvironment was insufficient, the degree of deshielding was limited, and it was difficult to achieve efficient exposure of the composite active core. Therefore, the effect of improving the disease resistance of chicken flocks was significantly lower than that of the preferred embodiment of the present invention.

[0039] Comparative Example 3: 0.58% of physically mixed encapsulated granules were added to the basic feed. The average molecular weight of the active components derived from Poria cocos in these granules was 15.8 kDa, and the cell wall breakage rate of the Ganoderma lucidum spore powder corresponding to the active components derived from Ganoderma lucidum spore powder was 95.0%. However, during the preparation process, the two active components were not pre-formed into a composite active core. Instead, the two types of active components were mixed with the coating material simultaneously and then encapsulated into granules in one step. The composition of the coating material was consistent with that of Example 5. Although the resulting sample had an encapsulated structure in appearance, due to the lack of a stable and directionally exposed composite active core interface, even if the coating layer became somewhat loose under pathological conditions, the synergistic exposure and concentration of the active ingredients were still insufficient. Therefore, it was inferior to the embodiments of the present invention in terms of reducing morbidity, reducing bacterial load, and improving immune indicators. Figure 1 As shown, the host-response triggered functional particles constructed in this invention, by setting a composite active core and a two-layer multi-factor coupled response structure consisting of an internal response regulation layer and an external response shielding layer, can maintain good shielding stability in a healthy intestinal environment and achieve more complete deshielding and active core exposure in the pathological microenvironment related to infection or inflammation. In contrast, control schemes without a response layer, single-layer coating, or without pre-forming a composite active core are all difficult to balance the protective effect in a healthy environment with the targeted release in a pathological environment, and therefore have a weaker overall anti-disease effect.

[0040] Table 1. Comparison of in vitro response effects between the examples and comparative examples.

[0041] Table 1 shows a comparison of the release effects of each embodiment and the comparative example in healthy gut microenvironment simulated solution and pathological microenvironment simulated solution, used to evaluate the shielding stability of the samples under healthy conditions and their ability to trigger release under infection or inflammation-related conditions. As can be seen from Table 1, the release rates of each embodiment of the present invention are low in the healthy environment, while the release rates are significantly higher in the pathological environment, and the overall pathological / healthy release fold is higher than that of the comparative example. This indicates that the present invention can achieve stable shielding under healthy conditions, targeted deshielding under pathological conditions, and exposure of active nuclei, exhibiting good environmental recognition and responsive release effects.

[0042] Table 2. Comparison of disease resistance in chicken flocks between the examples and the comparative examples.

[0043] Table 2 shows a comparison of the disease resistance effects of each embodiment and the comparative example under chicken-derived pathogenic Escherichia coli challenge conditions, including morbidity, cecal bacterial load, and serum IgA level. As can be seen from Table 2, each embodiment of the present invention can reduce morbidity and bacterial load after challenge and increase serum IgA level, with overall effects superior to the comparative example. This indicates that the present invention, through the structural design of host-response triggered functional particles, improves the release efficiency and utilization effect of active components in the pathological intestinal environment, thereby more effectively enhancing the disease resistance of chicken flocks.

[0044] The above description is only a specific embodiment of this disclosure, but the protection scope of this disclosure is not limited thereto. The protection scope of this disclosure should be determined by the protection scope of the claims.

Claims

1. A feed containing Poria cocos and Ganoderma lucidum spores for improving the disease resistance of chickens, characterized in that, The Poria cocos and Ganoderma lucidum spore powder feed includes a basic feed and host response triggered functional particles. The host response triggered functional particles include a composite active core and a multi-factor coupled response layer covering the outside of the composite active core. The composite active core includes active components derived from Poria cocos and active components derived from Ganoderma lucidum spore powder. The active components derived from Poria cocos are Poria cocos polysaccharides and / or Poria cocos polysaccharide degradation fragments, and the active components derived from Ganoderma lucidum spore powder are broken-cell wall Ganoderma lucidum spore powder, Ganoderma lucidum spore powder extract and / or Ganoderma lucidum spore powder conversion products. The multi-factor coupling response layer maintains a shielded state against the complex active core in the healthy intestinal environment of chickens, and generates a coupling response to at least two of the factors among the increased reactive oxygen species level, changes in intestinal pH, and changes in intestinal enzyme environment in the intestinal pathological microenvironment related to infection or inflammation in chickens. By generating a coupling response through two factors, the outer layer of host-response-triggered functional particles is deshielded, and the glycosyl interface derived from Poria cocos and / or the active interface derived from Ganoderma lucidum spore powder on the surface of the composite active core are exposed.

2. The Poria cocos and Ganoderma lucidum spore powder feed for improving disease resistance in chickens as described in claim 1, characterized in that, The active components derived from Poria cocos and the active components derived from Ganoderma lucidum spore powder are pre-formed into a composite active core, and the mass ratio of the active components derived from Poria cocos to the active components derived from Ganoderma lucidum spore powder in the composite active core is 1:(0.1-5).

3. The Poria cocos and Ganoderma lucidum spore powder feed for improving disease resistance in chickens as described in claim 2, characterized in that, The number-average molecular weight of the active components derived from Poria cocos is 0.5 kDa to 200 kDa; The cell wall breakage rate of the Ganoderma lucidum spore powder in the active components of the Ganoderma lucidum spore powder is over 70%.

4. The Poria cocos and Ganoderma lucidum spore powder feed for improving disease resistance in chickens as described in claim 1, characterized in that, The multi-factor coupled response layer includes an external response shielding layer and an internal response regulation layer; The external response shielding layer prevents premature exposure of the active nucleus in a healthy intestinal environment, while the internal response regulation layer undergoes at least one of the following changes in the intestinal pathological microenvironment related to infection or inflammation: degradation, dissociation, swelling, cracking, or rearrangement.

5. The Poria cocos and Ganoderma lucidum spore powder feed for improving the disease resistance of chicken flocks as described in claim 1 or 4, characterized in that, The multi-factor coupled response layer is any one of the following: reactive oxygen species response coupled with enzyme response, reactive oxygen species response coupled with pH response, and enzyme response coupled with pH response.

6. The Poria cocos and Ganoderma lucidum spore powder feed for improving disease resistance in chickens as described in claim 1, characterized in that, The host-response triggered functional pellets are added to the basal feed at a rate of 0.01% to 15%.

7. The method for preparing Poria cocos and Ganoderma lucidum spore powder feed for improving disease resistance in chicken flocks as described in any one of claims 1 to 6, characterized in that: Step 1: Extract and enzymatically hydrolyze the raw material of Poria cocos to obtain the active components derived from Poria cocos; Step 2: The Ganoderma lucidum spore powder is subjected to cell wall breaking and extraction processes to obtain the active components derived from the Ganoderma lucidum spore powder; Step 3: Combine and pre-assemble the active components from Poria cocos with the active components from Ganoderma lucidum spore powder to form a composite active core; Step 4: A responsive shielding layer is formed on the outside of the composite active core, generating host-response triggered functional particles; Step 5: Mix the host response-triggered functional particles with the basic feed to obtain Poria cocos and Ganoderma lucidum spore powder feed.

8. The preparation method according to claim 7, characterized in that, The formation of the composite active core is achieved by combining active components derived from Poria cocos with active components derived from Ganoderma lucidum spore powder and then assembling them.

9. The preparation method according to claim 7, characterized in that, The responsive shielding layer is formed by mixing the composite active core and the shielding layer components and attaching them to the outer surface of the composite active core; The shielding layer components attached to the outer surface of the composite active core are cured or cross-linked. The shielding layer forming component is one or more of polysaccharide coating materials, protein coating materials, or lipid coating materials.

10. The preparation method according to claim 7, characterized in that, The formation of the responsive shielding layer refers to first forming an inner response regulation layer outside the composite active core, and then forming an outer response shielding layer outside the inner response regulation layer.

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