A sampling and detection device and method for experimental solutions containing active substances from longan leaves

Abstract

This invention discloses a sampling and detection device for experimental solutions of active substances from longan leaves, specifically relating to the technical field of sampling and detection equipment for active substances from longan leaves. It includes a spectrometer and a housing. The left end of the housing is fixedly connected to the right end of the spectrometer. A flask fixing structure is provided at the upper end of the sampling bottle. A fixing drive component is jointly provided on the upper outer surface of the flask fixing structure and the top wall of the inner surface of the housing. A sampling drive structure is jointly provided on the outer surface of the fixing drive component and the bottom wall of the isolation chamber. This invention's sampling and detection device for experimental solutions of active substances from longan leaves, through the cooperation of the sampling drive structure and the tube rack, samples the experimental solution in the sampling bottle via an infusion tube, eliminating the need for manual sampling. Simultaneously, the flask fixing structure isolates the experimental solution in the sampling bottle from the external air, reducing the influence of the external environment on the experimental solution during sampling.

Description

Technical Field

[0001] This invention relates to the technical field of sampling and detection equipment for active substances in longan leaves, and particularly to a sampling and detection device and method for experimental solutions of active substances in longan leaves. Background Technology

[0002] Longan leaves are the leaves or tender shoots of the longan tree, a plant belonging to the Sapindaceae family and the Diospyros genus. They have the effects of clearing heat and detoxifying, dispelling wind and dampness. Studies have found that longan leaves contain relatively rich flavonoids, among which the main active ingredients are quercetin, quercetin glycoside, and kaempferol. Modern medical research has found that these active ingredients have the effect of controlling α-glucosidase activity, and therefore may have the effect of lowering blood sugar. In order to further study the active substances in longan leaves, samples of longan leaf raw materials containing these active substances need to be dissolved in chemical reagents for testing.

[0003] However, existing testing equipment requires laboratory personnel to manually hold the reagent bottle of the test solution and add it to the cuvette, and then place the cuvette into the spectrometer for analysis or to manually insert the spectrometer tube into the reagent bottle for sampling. Furthermore, a pipette is required during the addition process, and the experimental solution is constantly exposed to the air during this process. The active substances in the experimental solution will be continuously affected by other substances in the air. The reaction between active substances such as quercetin, quercetin, and kaempferol in the longan leaf experimental solution and impurities in the air will affect their component ratio, ultimately affecting the accuracy of spectral analysis of the longan leaf active substance experimental solution. Summary of the Invention

[0004] The main objective of this invention is to provide a sampling and detection device and method for experimental solutions containing active substances from longan leaves, which can effectively solve the problems of existing sampling and detection devices requiring manual sampling and the experimental solutions being exposed to air during the sampling process and easily affected by other substances in the environment.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A sampling and detection device for experimental solutions of active substances from longan leaves includes a spectrometer and a housing. The left end of the housing is fixedly connected to the right end of the spectrometer. An isolation chamber is fixedly connected to the upper end of the housing. A sampling bottle is placed on the bottom wall of the inner surface of the housing. A flask fixing structure is provided on the upper end of the sampling bottle. A fixing drive component is provided on the upper part of the outer surface of the flask fixing structure and the top wall of the inner surface of the housing. A sampling drive structure is provided on the outer surface of the fixing drive component and the bottom wall of the inner cavity of the isolation chamber. A tube winding frame is fixedly connected to the bottom wall of the inner cavity of the isolation chamber. An infusion tube is wound around the inner surface of the tube winding frame and is slidably connected to the inner surfaces of the fixing drive component, the flask fixing structure, and the sampling drive structure. The end of the infusion tube away from the sampling bottle is fixedly connected to the inlet of the housing.

[0006] Preferably, the sampling drive structure includes a support platform and a motor bracket fixedly connected to the bottom wall of the isolation chamber. A drive motor is slidably connected to the inner surface of the motor bracket through a limiting groove. A tube delivery assembly is fixedly connected to the output end of the drive motor. A pressing assembly is provided on the bottom wall of the inner surface of the isolation chamber and threadedly connected to the fixed drive component.

[0007] Preferably, the tube delivery assembly includes a rubber wheel fixedly connected to the output end of the drive motor and tube delivery wheels symmetrically mounted on the upper part of the support platform via bearing brackets. The outer surfaces of both tube delivery wheels are in close contact with the outer surface of the infusion tube. A friction wheel is fixedly connected to the rear end of each of the two tube delivery wheels. A sliding block is fixedly connected to the end of the rubber wheel away from the drive motor via a rotating shaft. The inner surface of the sliding block is slidably connected to the flask fixing structure.

[0008] Preferably, the pressing assembly includes a rack that is slidably connected to the bottom wall of the isolation cavity, the front end of the rack being engaged with a gear column that is rotatably connected to the bottom wall of the isolation cavity, the lower end of the gear column extending through the bottom wall of the isolation cavity to the upper wall of the housing cavity, the inner surface of the gear column being threadedly connected to a fixed drive component, and the upper end of the rack being in close contact with the outer surface of the rubber wheel.

[0009] Preferably, the fixed drive component includes a threaded column that is threaded to the inner surface of the gear column, and a wedge-shaped slide is fixedly connected to the lower end of the threaded column. The lower end of the wedge-shaped slide has several notches distributed in a ring. The lower end of the threaded column and the inner cavity of the wedge-shaped slide are slidably connected to the flask fixing structure.

[0010] Preferably, the flask fixing structure includes a second compression spring fixedly connected to the lower end of the threaded column, a pressing block fixedly connected to the lower end of the second compression spring and slidably connected to the inner surface of the wedge-shaped slide, a plurality of clamping components corresponding to the notches being arranged in a ring on the outer surface of the pressing block, a guide component slidably connected to the inner surface of the threaded column being slidably connected to the inner surface of the pressing block, and an isolation component being provided on the outer surface of the guide component located at the lower part of the pressing block.

[0011] Preferably, the guiding assembly includes a guiding tube slidably connected to the inner surface of the extrusion block and a limiting rod fixedly connected to the middle of the outer surface of the guiding tube. The outer surface of the guiding tube located in the inner cavity of the extrusion block has several wedge-shaped grooves distributed in a ring. The upper part of the outer surface of the guiding tube located in the inner cavity of the threaded column is symmetrically fixedly connected to a compression spring three fixedly connected to the top wall of the inner surface of the threaded column. The upper end of the limiting rod extends through the inner cavity of the threaded column to the inner cavity of the isolation cavity and is fixedly connected to a limiting plate that is in close contact with the lower end of the sliding block.

[0012] Preferably, the clamping assembly includes a plurality of tension springs arranged in a ring and fixedly connected to the outer surface of the extrusion block. Each of the tension springs has a clamping claw fixedly connected to the end away from the extrusion block. The outer surface of the clamping claw is rotatably connected to the outer surface of the extrusion block through a bracket. The inner surface of the clamping claw is in close contact with a slide rod that is slidably connected to the inner cavity of the extrusion block. The end of the slide rod away from the clamping claw is in close contact with the inclined surface of the inner surface of the adjacent wedge groove.

[0013] Preferably, the isolation assembly includes a limiting ring fixedly connected to the lower end of the extrusion block, a rubber airbag fixedly connected to the lower end of the limiting ring, the inner surfaces of the limiting ring and the rubber airbag being slidably connected to the outer surface of the guide tube, and a fixing ring fixedly connected to the lower part of the outer surface of the guide tube.

[0014] In addition, the present invention also provides a detection method using a sampling and detection device for experimental solutions of active substances from longan leaves, the method comprising: S1. First, place the longan leaf extract in the sampling bottle, put the sampling bottle into the bottom wall of the inner cavity of the shell, start the spectrometer, and control the drive motor to rotate the rubber wheel through the spectrometer. The rotation of the rubber wheel drives the rack to move and drives the gear column to rotate. The gear column drives the threaded column to move downward through the threaded groove until the squeezing block contacts the upper end of the sampling bottle and gradually presses it.

[0015] S2. When the squeezing block contacts the upper end of the sampling bottle, the guide tube and the limiting rod rise relative to the threaded column under the action of the compression spring two. The wedge-shaped slide presses the gripper, causing its lower part to move inward and clamping the sampling bottle. At the same time, the rise of the guide tube drives the fixing ring to rise, flattening the limiting ring so that it is tightly attached to the inner wall of the sampling bottle. The sliding block drives the rubber wheel to rise and contact the friction wheel one. The rotation of the tube delivery wheel drives the infusion tube to extend along the guide tube into the sampling bottle, and the extract is drawn into the shell through the infusion tube. The experimental solution of longan leaf active matter in the sampling bottle is subjected to spectral analysis, and the spectral analysis data is read by the spectrometer.

[0016] S3. After the analysis is completed, the drive motor drives the rubber wheel to reverse, the tube delivery wheel drives the infusion tube to move upward and wrap around the tube rack. At the same time, the gear column reverses and drives the threaded column to rise. The wedge slide separates from the gripper, the tension spring resets the gripper, and simultaneously, under the action of the guide tube and the compression spring, the isolation component and the slide bar are reset, and the sampling bottle is taken out.

[0017] Compared with the prior art, the present invention has the following beneficial effects: 1. This invention controls the operation of the sampling drive structure using a spectrometer. The fixed drive component and the sampling drive structure work together to lower the flask fixing structure and fix the sampling bottle in the shell. The sampling drive structure works with the tube rack to sample the experimental solution in the sampling bottle through the infusion tube, eliminating the need for manual sampling. At the same time, the flask fixing structure isolates the experimental solution in the sampling bottle from the outside air, reducing the influence of the external environment on the experimental solution in the sampling bottle during the sampling process.

[0018] 2. This invention utilizes the interaction between the rubber wheel and the rack in the sampling drive structure to drive the fixed drive component downwards via the action of the gear column. During the downward movement of the fixed drive component, the flask fixing structure is brought into close contact with the upper end of the sampling bottle. When the flask fixing structure is in close contact with the upper end of the sampling bottle, the sliding block, in conjunction with the flask fixing structure, drives the rubber wheel upwards to come into close contact with the friction wheel, thereby causing the friction wheel to rotate. This pressure causes the infusion tube to extend downwards into the sampling bottle to sample the experimental solution of longan leaf active ingredients. This reduces manual intervention, minimizes contamination of the experimental solution during sampling, and improves the accuracy and efficiency of detection.

[0019] 3. The present invention uses the mutual cooperation of the threaded column and the gear column to drive the wedge slide to move downward. The interaction between the wedge slide and the clamping component allows the squeezing block to contact the upper end of the sampling bottle and clamp and fix the sampling bottle through the clamping component, which facilitates subsequent sampling.

[0020] 4. This invention utilizes the cooperation between the wedge-shaped slide and the guide tube. After the extrusion block is in place, the compression spring II causes the guide tube to rise relative to the threaded column. At this time, since the limiting ring and the extrusion block are relatively fixed, when the fixing ring rises with the guide tube, it will compress the rubber airbag and flatten it. At this time, both sides of the rubber airbag are in close contact with the inner wall of the sampling bottle, isolating the experimental solution inside the sampling bottle from the external air and reducing the influence of the external environment on the components in the experimental solution. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic cross-sectional view of the internal structure of the isolation cavity of the present invention; Figure 3 This is a schematic diagram of the sampling drive structure of the present invention; Figure 4 This is a schematic diagram of the structure of the tube feeding assembly and the pressing assembly of the present invention; Figure 5 This is a schematic diagram showing the connection relationship between the sampling drive structure and the flask fixing structure of the present invention; Figure 6 This is a cross-sectional schematic diagram of the flask fixing structure of the present invention; Figure 7 This is a schematic diagram showing the positional relationship between the guiding component and the clamping component of the present invention; Figure 8 This is a schematic diagram showing the positional relationship between the guiding component and the isolation component of the present invention; Figure 9 For the present invention Figure 8 Enlarged schematic diagram of a local structure at point A; Figure 10 This is a schematic diagram of another state of the present invention.

[0022] In the diagram: 1. Spectrometer; 2. Housing; 21. Isolation chamber; 3. Fixed drive component; 31. Threaded column; 32. Wedge-shaped slide; 33. Notch; 4. Flask fixing structure; 41. Squeezing block; 42. Guide assembly; 421. Guide tube; 422. Limiting rod; 423. Wedge groove; 424. Limiting plate; 425. Compression spring three; 43. Clamping assembly; 431. Tension spring; 432. Gripper; 433. Slide rod; 44. Isolation chamber Components: 441, Limiting ring; 442, Rubber airbag; 443, Fixing ring; 45, Compression spring II; 5, Sampling drive structure; 51, Support platform; 52, Tube delivery assembly; 521, Friction wheel I; 522, Tube delivery wheel; 523, Rubber wheel; 524, Sliding block; 53, Drive motor; 54, Motor bracket; 55, Pressing assembly; 551, Spur rack; 552, Gear column; 6, Tube winding frame; 61, Infusion tube; 7, Sampling bottle. Detailed Implementation

[0023] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0024] Example 1 like Figure 1 and Figure 2 As shown, a sampling and detection device for experimental solutions of active substances from longan leaves includes a spectrometer 1 and a housing 2. The spectrometer 1 is fixedly connected to the left end of the housing 2 and the right end of the housing 2. An isolation chamber 21 is fixedly connected to the upper end of the housing 2. A sampling bottle 7 is placed on the bottom wall of the inner surface of the housing 2. A flask fixing structure 4 is provided on the upper end of the sampling bottle 7. A fixing drive component 3 is provided on the upper part of the outer surface of the flask fixing structure 4 and the top wall of the inner surface of the housing 2. A sampling drive structure 5 is provided on the outer surface of the fixing drive component 3 and the bottom wall of the inner cavity of the isolation chamber 21. A tube rack 6 is fixedly connected to the bottom wall of the inner cavity of the isolation chamber 21. An infusion tube 61 is wound around the inner surface of the tube rack 6 and is slidably connected to the inner surfaces of the fixing drive component 3, the flask fixing structure 4, and the sampling drive structure 5. The end of the infusion tube 61 away from the sampling bottle 7 is fixedly connected to the inlet of the housing 2.

[0025] It should be noted that the aforementioned shell 2 is a scientific instrument capable of performing spectral analysis on the introduced liquid. It is a commonly used analytical instrument in modern chemical and medical experiments. By capturing light information, developing it with photographic film, or displaying and analyzing the numerical values ​​using computerized automatic display instruments, it can determine the elements contained in the substance. It has been widely used in modern detection fields. In this invention, its working principle is used to detect and analyze the proportion of active substances such as quercetin, quercetin, and kaempferol in the experimental solution of longan leaf active substances. Its specific internal structure and operating principle will not be elaborated in detail.

[0026] Additionally, the tube winding frame 6 is a winding frame with a built-in spring clip, which can wind up the infusion tube 61 without external force. It is mainly used to retrieve the infusion tube 61 in conjunction with the sampling drive structure 5 after the test is completed. Its specific structure is not described in detail.

[0027] In this embodiment, the sampling bottle 7 is first placed in the housing 2. The sampling drive structure 5 is controlled by the spectrometer 1. The fixed drive component 3 and the sampling drive structure 5 work together to drive the flask fixing structure 4 to descend and fix the sampling bottle 7 in the housing 2. The experimental solution in the sampling bottle 7 is sampled through the infusion tube 61 by the sampling drive structure 5 and the tube rack 6, without the need for personnel to hold the sample. At the same time, the flask fixing structure 4 is used to isolate the experimental solution in the sampling bottle 7 from the outside air, reducing the influence of the external environment on the experimental solution in the sampling bottle 7 during the sampling process.

[0028] Example 2 Based on Example 1, this embodiment utilizes the interaction between the rubber wheel 523 and the rack 551 in the sampling drive structure 5, which, through the action of the gear column 552, drives the fixed drive component 3 downward. During the downward movement of the fixed drive component 3, the flask fixing structure 4 is brought into close contact with the upper end of the sampling bottle 7. When the flask fixing structure 4 and the upper end of the sampling bottle 7 are in close contact, the interaction between the sliding block 524 and the flask fixing structure 4 drives the rubber wheel 523 upward to bring it into close contact with the friction wheel 521. This causes the friction wheel 521 to rotate, which in turn compresses the infusion tube 61, extending it downward into the sampling bottle 7 to sample the experimental solution of longan leaf active ingredients. This reduces manual intervention, minimizes contamination of the experimental solution during sampling, and improves the accuracy and efficiency of the detection.

[0029] Specifically, to drive the operation of subsequent structures, please refer to... Figure 3 , Figure 4 and Figure 5The sampling drive structure 5 includes a support platform 51 and a motor bracket 54 that are fixedly connected to the bottom wall of the inner cavity of the isolation chamber 21. A drive motor 53 is slidably connected to the inner surface of the motor bracket 54 through a limiting groove. A tube feeding assembly 52 is fixedly connected to the output end of the drive motor 53. A pressing assembly 55 that is threadedly connected to the fixed drive component 3 is provided on the bottom wall of the inner surface of the isolation chamber 21.

[0030] The main purpose of setting up the motor bracket 54 is to limit the drive motor 53, so that the drive motor 53 can slide while driving the tube feeding assembly 52.

[0031] Furthermore, in order to drive the fixed drive component 3 downward by pressing down component 55, and at the same time deliver the infusion tube 61 into the sampling bottle 7, see [reference needed]. Figure 4 The tube delivery assembly 52 includes a rubber wheel 523 fixedly connected to the output end of the drive motor 53 and tube delivery wheels 522 symmetrically mounted on the upper end of the support platform 51 via bearing brackets. The outer surfaces of the two tube delivery wheels 522 are in close contact with the outer surface of the infusion tube 61. Friction wheels 521 are fixedly connected to the rear ends of the two tube delivery wheels 522. A sliding block 524 is fixedly connected to the end of the rubber wheel 523 away from the drive motor 53 via a rotating shaft. The inner surface of the sliding block 524 is slidably connected to the flask fixing structure 4.

[0032] Furthermore, to drive the fixed drive component 3 downward, refer to... Figure 4 The pressing assembly 55 includes a rack 551 that is slidably connected to the bottom wall of the inner cavity of the isolation chamber 21. The front end of the rack 551 is engaged with a gear column 552 that is rotatably connected to the bottom wall of the inner cavity of the isolation chamber 21. The lower end of the gear column 552 extends through the bottom wall of the inner cavity of the isolation chamber 21 to the upper wall of the inner cavity of the housing 2. The inner surface of the gear column 552 is threadedly connected to the fixed drive component 3. The upper end of the rack 551 is in close contact with the outer surface of the rubber wheel 523.

[0033] In summary, the drive motor 53 and the motor bracket 54 are slidably connected. When the sliding block 524 moves upward under the action of the flask fixing structure 4, it will drive the drive motor 53 to slide in the motor bracket 54 through the rubber wheel 523. When the sliding block 524 lifts the rubber wheel 523, the rubber wheel 523 will be in close contact with the two friction wheels 521. Under the action of friction, the friction wheels 521 will drive the tube delivery wheel 522 to rotate. The two tube delivery wheels 522 will squeeze and pull the infusion tube 61 downward until it enters the active substance solution.

[0034] Example 3 Based on Embodiment 2, this embodiment uses the mutual cooperation of the threaded column 31 and the gear column 552 to drive the wedge slide 32 to move downward. The interaction between the wedge slide 32 and the clamping assembly 43 is used to clamp and fix the sampling bottle 7 when the squeezing block 41 contacts the upper end of the sampling bottle 7. During the preparation of the experimental solution of longan leaves, certain suspended matter will be generated in the solution due to contact and reaction with air and reaction reagents. In the conventional sampling process, the sampling bottle 7 is held by hand and a pipette is used for sampling, which will cause some shaking, causing the suspended matter to enter the spectrometer 2 and affect the sampling accuracy. In this invention, 3 and 4 are used to fix the sampling bottle 7 to avoid shaking during the sampling process, thereby improving the detection accuracy of active ingredients in longan leaves.

[0035] Specifically, to drive the flask fixing structure 4 downwards, refer to... Figure 4 and Figure 5 The fixed drive component 3 includes a threaded column 31 that is threaded to the inner surface of the gear column 552. A wedge-shaped slide 32 is fixedly connected to the lower end of the threaded column 31. Several notches 33 are distributed in a ring at the lower end of the wedge slide 32. The lower end of the threaded column 31 and the inner cavity of the wedge slide 32 are slidably connected to the flask fixing structure 4.

[0036] When the gear column 552 rotates, the threaded column 31 will move downward under the limiting action of the guide component 42, and synchronously drive the wedge slide 32 to move downward until the wedge slide 32 contacts the clamping component 43. When the drive motor 53 stops running, the threaded connection has a self-locking property, which can maintain the fixation of the sampling bottle 7.

[0037] Furthermore, as the wedge-shaped slide 32 continues to move downwards, refer to... Figure 5 and Figure 6 The flask fixing structure 4 includes a compression spring 45 fixedly connected to the lower end of the threaded column 31. The lower end of the compression spring 45 is fixedly connected to a pressing block 41 that is slidably connected to the inner surface of the wedge-shaped slide 32. Several clamping components 43 corresponding to the notches 33 are arranged in a ring on the outer surface of the pressing block 41. A guide component 42 that is slidably connected to the inner surface of the threaded column 31 is slidably connected to the inner surface of the pressing block 41. An isolation component 44 is provided on the outer surface of the guide component 42 located at the lower part of the pressing block 41.

[0038] The threaded column 31 presses the extrusion block 41 downward through the compression spring 45 until the bottom of the extrusion block 41 contacts the top of the sampling bottle 7. At this time, the compression spring 45 is gradually compressed, and the inner wall of the wedge slide 32 slides downward along the outer surface of the extrusion block 41 until the wedge slide 32 contacts the clamping assembly 43 and presses the clamping assembly 43 to rotate, so that it is fastened to the mouth of the sampling bottle 7 to fix the sampling bottle 7.

[0039] Furthermore, to guide the infusion tube 61 into the sampling bottle 7 for liquid extraction, and simultaneously to allow the isolation component 44 to expand and isolate the experimental solution in the sampling bottle 7 through cooperation with the clamping component 43, see [reference needed]. Figure 6 , Figure 7 , Figure 8 and Figure 9 The guide assembly 42 includes a guide tube 421 that is slidably connected to the inner surface of the extrusion block 41 and a limiting rod 422 that is fixedly connected to the middle of the outer surface of the guide tube 421. The outer surface of the guide tube 421 located in the inner cavity of the extrusion block 41 has several wedge-shaped grooves 423 distributed in a ring. The upper part of the outer surface of the guide tube 421 located in the inner cavity of the threaded column 31 is symmetrically fixedly connected to a compression spring 425 that is fixedly connected to the top wall of the inner surface of the threaded column 31. The upper end of the limiting rod 422 extends through the inner cavity of the threaded column 31 to the inner cavity of the isolation cavity 21 and is fixedly connected to a limiting plate 424 that is in close contact with the lower end of the sliding block 524.

[0040] When the extrusion block 41 is in place, the limit rod 422 will move upward in sync with the guide tube 421. At this time, it will drive the sliding block 524 to move upward, and the rubber wheel 523 will drive the friction wheel 521 to rotate.

[0041] Furthermore, to clamp and secure the sampling bottle 7, refer to... Figure 6 , Figure 7 and Figure 8 The clamping assembly 43 includes a plurality of tension springs 431 arranged in a ring and fixedly connected to the outer surface of the extrusion block 41. Each tension spring 431 is fixedly connected to a claw 432 at the end away from the extrusion block 41. The outer surface of the claw 432 is rotatably connected to the outer surface of the extrusion block 41 through a bracket. The inner surface of the claw 432 is in close contact with a slide rod 433 that is slidably connected to the inner cavity of the extrusion block 41. The end of the slide rod 433 away from the claw 432 is in close contact with the inclined surface of the inner surface of the adjacent wedge groove 423.

[0042] In summary, the guide tube 421 will first move downward under the action of the threaded column 31 and the compression spring 425. When the squeezing block 41 stops moving, the wedge slide 32 will press down on the gripper 432 to make its lower part rotate inward. Through the cooperation of the gripper 432, the sampling bottle 7 is fixed in the housing 2. Simultaneously, as the gripper 432 moves inward, it will press the slide rod 433 to move inward. The part of the slide rod 433 that contacts the wedge groove 423 is equipped with a pulley. Through the cooperation of the slide rod 433 and the wedge groove 423, the guide tube 421 rises relative to the squeezing block 41, thereby driving the isolation component 44 to move. When the gripper 432 is pressed by the wedge-shaped slide 32, it will rotate around the middle of the gripper 432 so that the lower part of the gripper 432 is engaged with the wall of the sampling bottle 7. At this time, the tension spring 431 is stressed and accumulates elastic potential energy. When the wedge-shaped slide 32 rises, the gripper 432 returns to its original position under the action of the tension spring 431. Simultaneously, since the gripper 432 no longer presses the slide bar 433, the guide tube 421 will move downward under the action of the rubber air bag 442 and the compression spring 425, and press the slide bar 433 outward to return to its original position through the wedge groove 423.

[0043] Example 4 Based on Example 3, this embodiment utilizes the cooperation between the wedge-shaped slide 32 and the guide tube 421. After the extrusion block 41 is in place, the compression spring 45 causes the guide tube 421 to rise relative to the threaded column 31. At this time, since the limiting ring 441 is relatively fixed to the extrusion block 41, the fixing ring 443 rises with the guide tube 421, which will compress the rubber airbag 442 and flatten it. At this time, both sides of the rubber airbag 442 are in close contact with the inner wall of the sampling bottle 7, isolating the experimental solution inside the sampling bottle 7 from the external air, reducing the influence of the external environment on the active ingredients in the experimental solution. Oxygen and moisture in the air will react with the experimental solution, thereby affecting the component content in the longan leaf active extract.

[0044] Specifically, when the squeezing block 41 presses down on the upper end of the sampling bottle 7, the experimental solution in the sampling bottle 7 is separated from the external environment. (See [reference needed]). Figure 6 , Figure 7 and Figure 8 The isolation component 44 includes a limiting ring 441 fixedly connected to the lower end of the extrusion block 41. A rubber airbag 442 is fixedly connected to the lower end of the limiting ring 441. The inner surfaces of the limiting ring 441 and the rubber airbag 442 are slidably connected to the outer surface of the guide tube 421. A fixing ring 443 is fixedly connected to the lower part of the outer surface of the guide tube 421.

[0045] When the guide tube 421 and the squeezing block 41 descend synchronously under the action of the compression spring 45, the fixing ring 443 and the limiting ring 441 will drive the rubber airbag 442 to descend. When the lower end of the squeezing block 41 is in close contact with the upper end of the sampling bottle 7, the gripper 432 squeezes the slide bar 433 inward. The slide bar 433 drives the guide tube 421 to rise relative to the squeezing block 41. At this time, since the limiting ring 441 is fixed together with the squeezing block 41, the rubber airbag 442 will be flattened under the interaction of the limiting ring 441 and the fixing ring 443 until the two sides of the rubber airbag 442 are in close contact with the inner wall of the sampling bottle 7, thus isolating the solution in the sampling bottle 7.

[0046] Example 5 Based on Examples 1 to 4, the present invention also provides a detection method using a sampling and detection device for experimental solutions of active substances from longan leaves, the method comprising: S1. First, place the longan leaf extract in the sampling bottle 7, put the sampling bottle 7 into the bottom wall of the inner cavity of the shell 2, start the spectrometer 1, and control the drive motor 53 to run through the spectrometer 1 to drive the rubber wheel 523 to rotate. The rotation of the rubber wheel 523 drives the rack 551 to move and drives the gear column 552 to rotate. The gear column 552 drives the thread column 31 to move downward through the threaded groove until the squeezing block 41 contacts the upper end of the sampling bottle 7 and gradually presses it. S2. When the squeezing block 41 contacts the upper end of the sampling bottle 7, under the action of the compression spring 45, the guide tube 421 and the limiting rod 422 rise relative to the threaded column 31. The wedge-shaped slide 32 presses the gripper 432 so that its lower part moves inward, clamping the sampling bottle 7. At the same time, the rise of the guide tube 421 drives the fixing ring 443 to rise, flattening the limiting ring 441 so that it is tightly attached to the inner wall of the sampling bottle 7. The sliding block 524 drives the rubber wheel 523 to rise and contact the friction wheel 521. The rotation of the tube delivery wheel 522 drives the infusion tube 61 to extend along the guide tube 421 into the sampling bottle 7, and the extract is drawn into the shell 2 through the infusion tube 61. The experimental solution of longan leaf active matter in the sampling bottle 7 is subjected to spectral analysis, and the spectral analysis data is read by the spectrometer 1. S3. After the analysis is completed, refer to Figure 10 The drive motor 53 drives the rubber wheel 523 to reverse, and the tube delivery wheel 522 drives the infusion tube 61 to move upward and wrap around the tube rack 6. At the same time, the gear column 552 reverses and drives the threaded column 31 to rise. The wedge slide 32 separates from the gripper 432, and the tension spring 431 resets the gripper 432. Simultaneously, under the action of the guide tube 421 and the compression spring 425, the isolation component 44 and the slide bar 433 are reset, and the sampling bottle 7 is taken out.

[0047] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.

Claims

1. A sampling and detection device for experimental solutions of active substances from longan leaves, comprising a spectrometer (1) and a housing (2), characterized in that: The left end of the housing (2) is fixedly connected to the right end of the spectrometer (1). An isolation cavity (21) is fixedly connected to the upper end of the housing (2). A sampling bottle (7) is placed on the bottom wall of the inner surface of the housing (2). A flask fixing structure (4) is provided on the upper end of the sampling bottle (7). A fixing drive component (3) is provided on the upper part of the outer surface of the flask fixing structure (4) and the top wall of the inner surface of the housing (2). A sampling drive structure (5) is provided on the outer surface of the fixing drive component (3) and the bottom wall of the inner cavity of the isolation cavity (21). A tube rack (6) is fixedly connected to the bottom wall of the inner cavity of the isolation cavity (21). An infusion tube (61) is wound around the inner surface of the tube rack (6) and is slidably connected to the inner surfaces of the fixing drive component (3), the flask fixing structure (4), and the sampling drive structure (5). The end of the infusion tube (61) away from the sampling bottle (7) is fixedly connected to the inlet of the housing (2).

2. The sampling and detection device for experimental solutions of active substances in longan leaves according to claim 1, characterized in that: The sampling drive structure (5) includes a support platform (51) and a motor bracket (54) fixedly connected to the bottom wall of the inner cavity of the isolation chamber (21). The inner surface of the motor bracket (54) is slidably connected to a drive motor (53) through a limiting groove. The output end of the drive motor (53) is fixedly connected to a tube feeding assembly (52). The bottom wall of the inner surface of the isolation chamber (21) is provided with a pressing assembly (55) that is threadedly connected to the fixed drive component (3).

3. The sampling and detection device for experimental solutions of active substances in longan leaves according to claim 2, characterized in that: The tube delivery assembly (52) includes a rubber wheel (523) fixedly connected to the output end of the drive motor (53) and tube delivery wheels (522) symmetrically mounted on the upper end of the support platform (51) via bearing brackets. The outer surfaces of the two tube delivery wheels (522) are in close contact with the outer surface of the infusion tube (61). The rear ends of the two tube delivery wheels (522) are fixedly connected to friction wheels (521). The end of the rubber wheel (523) away from the drive motor (53) is fixedly connected to a sliding block (524) via a rotating shaft. The inner surface of the sliding block (524) is slidably connected to the flask fixing structure (4).

4. The sampling and detection device for experimental solutions of active substances in longan leaves according to claim 3, characterized in that: The pressing assembly (55) includes a rack (551) that is slidably connected to the bottom wall of the inner cavity of the isolation chamber (21). The front end of the rack (551) is engaged with a gear column (552) that is rotatably connected to the bottom wall of the inner cavity of the isolation chamber (21). The lower end of the gear column (552) extends through the bottom wall of the inner cavity of the isolation chamber (21) to the upper wall of the inner cavity of the housing (2). The inner surface of the gear column (552) is threadedly connected to the fixed drive component (3). The upper end of the rack (551) is in close contact with the outer surface of the rubber wheel (523).

5. The sampling and detection device for experimental solutions of active substances in longan leaves according to claim 4, characterized in that: The fixed drive component (3) includes a threaded column (31) that is threaded to the inner surface of the gear column (552). A wedge-shaped slide (32) is fixedly connected to the lower end of the threaded column (31). Several notches (33) are distributed in a ring at the lower end of the wedge-shaped slide (32). The lower end of the threaded column (31) and the inner cavity of the wedge-shaped slide (32) are slidably connected to the flask fixing structure (4).

6. The sampling and detection device for experimental solutions of active substances in longan leaves according to claim 5, characterized in that: The flask fixing structure (4) includes a compression spring two (45) fixedly connected to the lower end of the threaded column (31). The lower end of the compression spring two (45) is fixedly connected to a pressing block (41) that is slidably connected to the inner surface of the wedge-shaped slide (32). The outer surface of the pressing block (41) is provided with a plurality of clamping components (43) corresponding to the notches (33). The inner surface of the pressing block (41) is slidably connected to a guide component (42) that is slidably connected to the inner surface of the threaded column (31). The outer surface of the guide component (42) located at the lower part of the pressing block (41) is provided with an isolation component (44).

7. The sampling and detection device for experimental solutions of active substances in longan leaves according to claim 6, characterized in that: The guiding assembly (42) includes a guiding tube (421) slidably connected to the inner surface of the extrusion block (41) and a limiting rod (422) fixedly connected to the middle of the outer surface of the guiding tube (421). The outer surface of the guiding tube (421) located in the inner cavity of the extrusion block (41) is provided with a plurality of wedge-shaped grooves (423) distributed in a ring. The upper part of the outer surface of the guiding tube (421) located in the inner cavity of the threaded column (31) is symmetrically fixedly connected with a compression spring three (425) fixedly connected to the top wall of the inner surface of the threaded column (31). The upper end of the limiting rod (422) extends through the inner cavity of the threaded column (31) to the inner cavity of the isolation cavity (21) and is fixedly connected with a limiting plate (424) that is in close contact with the lower end of the sliding block (524).

8. The sampling and detection device for experimental solutions of active substances in longan leaves according to claim 7, characterized in that: The clamping assembly (43) includes a plurality of tension springs (431) arranged in a ring and fixedly connected to the outer surface of the extrusion block (41). Each of the tension springs (431) has a clamp (432) fixedly connected to one end away from the extrusion block (41). The outer surface of the clamp (432) is rotatably connected to the outer surface of the extrusion block (41) through a bracket. The inner surface of the clamp (432) is in close contact with a slide rod (433) that is slidably connected to the inner cavity of the extrusion block (41). The end of the slide rod (433) away from the clamp (432) is in close contact with the inclined surface of the inner surface of the adjacent wedge groove (423).

9. The sampling and detection device for experimental solutions of active substances in longan leaves according to claim 8, characterized in that: The isolation assembly (44) includes a limiting ring (441) fixedly connected to the lower end of the extrusion block (41), and a rubber airbag (442) fixedly connected to the lower end of the limiting ring (441). The inner surfaces of the limiting ring (441) and the rubber airbag (442) are slidably connected to the outer surface of the guide tube (421). The lower end of the rubber airbag (442) is fixedly connected to a fixing ring (443) fixedly connected to the lower part of the outer surface of the guide tube (421).

10. A detection method using the sampling and detection device for the experimental solution of active substances in longan leaves according to any one of claims 1-9, characterized in that, The method includes: S1. First, place the longan leaf extract in the sampling bottle (7), place the sampling bottle (7) into the bottom wall of the inner cavity of the shell (2), start the spectrometer (1), and control the drive motor (53) through the spectrometer (1) to drive the rubber wheel (523) to rotate. The rotation of the rubber wheel (523) drives the straight rack (551) to move and drives the gear column (552) to rotate. The gear column (552) drives the thread column (31) to move downward through the thread groove until the squeezing block (41) contacts the upper end of the sampling bottle (7) and gradually presses it. S2. When the squeezing block (41) contacts the upper end of the sampling bottle (7), under the action of the compression spring (45), the guide tube (421) and the limiting rod (422) rise relative to the threaded column (31), and the wedge-shaped slide (32) presses the gripper (432) to make its lower part move inward, clamping the sampling bottle (7). At the same time, the rise of the guide tube (421) drives the fixed ring (443) to rise, flattening the limiting ring (441) so that it is tightly attached to the sampling bottle (7). On the inner wall, the sliding block (524) drives the rubber wheel (523) to rise and contact the friction wheel (521). The rotation of the tube delivery wheel (522) drives the infusion tube (61) to extend into the sampling bottle (7) along the guide tube (421). The extract is drawn into the shell (2) through the infusion tube (61). The experimental solution of longan leaf active substance in the sampling bottle (7) is subjected to spectral analysis, and the spectral analysis data is read by the spectrometer (1). S3. After the analysis is completed, the drive motor (53) drives the rubber wheel (523) to reverse, the tube delivery wheel (522) drives the infusion tube (61) to move upward and wrap around the tube rack (6). At the same time, the gear column (552) reverses and drives the threaded column (31) to rise. The wedge slide (32) separates from the gripper (432), the tension spring (431) resets the gripper (432), and under the action of the guide tube (421) and the compression spring (425), the isolation component (44) and the slide rod (433) are reset, and the sampling bottle (7) is taken out.

Images