A pipette for liquid exchange in the cultivation of neural stem cells

Abstract

The application discloses a pipette for liquid replacement in the process of neural stem cell culture, and relates to the technical field of neural stem cell culture.The pipette comprises a pipe body and a rubber ball, the top of the pipe body is provided with a tapered port, the bottom of the pipe body is provided with a liquid inlet, the bottom of the rubber ball is fixedly connected with a tapered pipe, a filter screen is fixedly arranged in the liquid inlet, and the sidewall of the pipe body is provided with a cleaning mechanism for automatically cleaning the filter screen.The pipette for liquid replacement in the process of neural stem cell culture can make the culture solution enter the pipe body through the liquid inlet after being filtered by the filter screen.The arrangement of the filter screen can not only avoid the blockage of the pipette by cell clumps, but also avoid the waste of cell clumps, and ensure the culture effect.When the filter screen is blocked, the cell clumps at the bottom of the filter screen can be automatically scraped and cleaned, so that the blockage is avoided, and the efficiency and effect of pipetting are ensured.

Description

Technical Field

[0001] This invention relates to the field of neural stem cell culture technology, specifically to a pipette used for changing the medium during neural stem cell culture. Background Technology

[0002] In the process of neural stem cell culture, medium change is one of the core operations to maintain cell survival, proliferation, and stable biological characteristics. The pipette used for medium change needs to balance sterility, operational precision, and gentleness on the cells. It mainly consists of a tube body and a rubber bulb. The rubber bulb creates negative pressure inside the tube, allowing the culture medium to be drawn into the tube through the inlet. After the medium is drawn, the rubber bulb is removed, and the conical opening at the top of the tube body is quickly sealed with a finger. The culture medium can then be rotated. When it is necessary to drain the medium, the finger is removed.

[0003] However, when using existing pipettes for changing the medium during neural stem cell culture, cell clumps can easily clog the inlet, affecting the efficiency and effectiveness of pipetting. Furthermore, cell clumps can easily be extracted along with the culture medium, which can also affect the culture results.

[0004] Therefore, we propose a pipette for changing the medium during neural stem cell culture. Summary of the Invention

[0005] The purpose of this invention is to provide a pipette for changing the medium during neural stem cell culture, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a pipette for changing the medium during neural stem cell culture, comprising a tube body and a rubber bulb, wherein a conical opening is provided at the top of the tube body, an inlet is provided at the bottom of the tube body, and a conical tube is fixedly connected to the bottom of the rubber bulb; a filter screen is detachably connected inside the inlet, and an installation groove is provided inside the filter screen, a filter membrane is inserted into the installation groove, and a cleaning mechanism for automatically cleaning the filter screen is provided on the side wall of the tube body; The cleaning mechanism includes a fixed plate fixedly connected to the side wall of the pipe body, and a scraper is rotatably connected to the bottom of the fixed plate through a first rotating shaft. The rotation of the first rotating shaft is driven by a driving mechanism.

[0007] Preferably, the driving mechanism includes a protective cover fixedly connected to the top of the fixed plate, and a driving shaft is fixedly connected to the upper end of the first rotating shaft. The side wall of the driving shaft is provided with a sliding groove, and the sliding groove includes a plurality of V-shaped grooves connected end to end. A push pin is connected to the V-shaped groove through a lifting mechanism, and a limiting mechanism for limiting the driving shaft is provided on the side wall of the tube.

[0008] By adopting the above technical solution, the lifting mechanism drives the push pin to slide down along the sliding groove, which in turn drives the drive shaft to reciprocate.

[0009] Preferably, the lifting mechanism includes an L-shaped lifting rod inserted into the top of the protective cover, and a fixing ring is fixedly sleeved on the side wall of the tube. The top of the fixing ring is connected to a moving block through a first reset mechanism, and a connecting rod is fixedly connected to the bottom of the moving block. The lower end of the connecting rod is fixed to the top of the lifting rod, and the movement of the moving block is driven by a first pushing mechanism.

[0010] By adopting the above technical solution, when the filter screen becomes clogged, the first pushing mechanism pushes the moving block downward. When the moving block moves downward, the connecting rod and the lifting rod can drive the pushing pin to slide downward along the sliding groove.

[0011] Preferably, the first reset mechanism includes two symmetrically arranged sleeves fixedly connected to the top of the fixed ring, and a sleeve rod is inserted inside the sleeve. The upper end of the sleeve rod is fixed to the bottom of the moving block, and a first spring is sleeved on the side wall of each sleeve.

[0012] By adopting the above technical solution, the movement of the moving block is guided and reset.

[0013] Preferably, the first pushing mechanism includes a rectangular cover fixedly connected to the top of the fixed ring, and a pushing block is slidably connected inside the rectangular cover. The pushing block is fixed to the bottom of the moving block, and the rectangular cover is connected to the pipe body through a connecting pipe.

[0014] By adopting the above technical solution, when the filter screen becomes clogged, the air inside the rectangular cover can be sucked out through the connecting pipe, creating a negative pressure inside the rectangular cover. Under the action of the negative pressure, the pushing block can slide down along the rectangular cover, while the first spring is compressed.

[0015] Preferably, the limiting mechanism includes a plurality of limiting holes arranged in an array on the side wall of the drive shaft, the side wall of the tube is connected to a limiting rod through a second reset mechanism, and the movement of the limiting rod is driven by a second pushing mechanism.

[0016] By adopting the above technical solution, during normal use, the second pushing mechanism pushes the limiting rod into the limiting hole to limit the drive shaft and the first rotating shaft.

[0017] Preferably, the second reset mechanism includes a ring fixedly sleeved on the side wall of the limiting rod, the limiting rod extending into the tube body, and a second spring sleeved on the side wall of the limiting rod.

[0018] By adopting the above technical solution, the movement of the limit rod is guided and reset.

[0019] Preferably, the second pushing mechanism includes a rotating plate disposed above the filter screen, and the rotating plate is rotatably connected to the top of the filter screen through a rotating mechanism. Two symmetrically arranged first connecting blocks are fixedly connected to the side wall of the rotating plate, and a connecting rod is rotatably connected to the side wall of the first connecting block through a first rotating pin. Two symmetrically arranged second connecting blocks are fixedly connected to the end of the limiting rod, and the end of the connecting rod away from the first connecting block is rotatably connected to the side wall of the second connecting block through a second rotating pin.

[0020] By adopting the above technical solution, during normal use, when the culture medium enters the tube through the filter and inlet, it impacts the surface of the rotating plate. Under the action of the liquid impact force, the rotating plate can rotate clockwise along the rotating mechanism. At the same time, it drives the connecting rod to rotate and pushes the limiting rod to move and insert into the limiting hole. When the filter is blocked by cell clumps, the impact force of the liquid on the rotating plate is small, and the rotating plate will rotate counterclockwise along the second rotating shaft. The limiting rod can be withdrawn from the limiting hole under the action of the second spring. At this time, the drive shaft and the first rotating shaft are no longer limited.

[0021] Preferably, the rotating mechanism includes two symmetrically arranged support blocks fixedly connected to the top of the filter screen, and the rotating plate is rotatably connected to the side wall of the support blocks through a second rotating shaft.

[0022] By adopting the above technical solution, the rotational connection between the rotating plate and the filter screen is guaranteed.

[0023] Preferably, the bottom of the first rotating shaft and the scraper are provided with multiple mounting grooves, and ball bearings are provided in the mounting grooves.

[0024] By adopting the above technical solution, even when the scraper comes into contact with the bottom of the culture container, the ball bearing can first contact the bottom of the culture container, ensuring that the first rotating shaft and the scraper rotate normally.

[0025] In summary, Advantage 1: It allows the culture medium to pass through the filter screen and enter the tube through the inlet. The filter screen not only prevents cell clumps from clogging the pipette, but also avoids cell clump waste, ensuring the culture effect. Advantage 2: When the filter screen is clogged, it can automatically scrape and clean the cell clumps at the bottom of the filter screen to prevent clogging and ensure the efficiency and effectiveness of pipetting. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram showing the location of the cleaning mechanism in this invention; Figure 3This is a partial cross-sectional view of the tube body in this invention; Figure 4 for Figure 1 Enlarged view of point A in the middle; Figure 5 for Figure 1 Enlarged view at point B in the middle; Figure 6 for Figure 2 Enlarged view at point C; Figure 7 for Figure 3 Enlarged view at point D; Figure 8 for Figure 6 Enlarged view at point E in the middle; Figure 9 for Figure 7 Enlarged view of point F in the middle.

[0027] In the diagram: 101, pipe body; 102, rubber ball; 103, conical inlet; 104, liquid inlet; 105, conical tube; 201, drive shaft; 202, V-groove; 204, push pin; 205, protective cover; 301, lifting rod; 302, fixing ring; 303, moving block; 304, connecting rod; 401, sleeve; 402, sleeve rod; 403, first spring; 501, rectangular cover; 502, push block; 503, connecting tube; 601, mounting groove. ; 602, ball bearing; 701, limiting hole; 702, limiting rod; 801, ring; 802, second spring; 901, rotating plate; 902, connecting rod; 903, first connecting block; 904, first rotating pin; 905, second connecting block; 906, second rotating pin; 1001, support block; 1002, second rotating shaft; 11, filter screen; 1201, fixing plate; 1202, first rotating shaft; 1203, scraper; 13, mounting groove; 14, filter membrane. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Please see Figures 1-9The illustration shows a pipette used for changing the medium during neural stem cell culture, including a tube body 101 and a rubber ball 102. The top of the tube body 101 is provided with a conical opening 103, and the bottom of the tube body 101 is provided with a liquid inlet 104. The bottom of the rubber ball 102 is fixedly connected to a conical tube 105. A filter screen 11 is detachably connected inside the liquid inlet 104, and an installation groove 13 is opened inside the filter screen 11. A filter membrane 14 is inserted into the installation groove 13. The filter membrane 14 can reduce the loss of cell balls. The side wall of the tube body 101 is provided with a cleaning mechanism for automatically cleaning the filter screen 11. The cleaning mechanism includes a fixed plate 1201 fixedly connected to the side wall of the pipe body 101, and a scraper 1203 is rotatably connected to the bottom of the fixed plate 1201 via a first rotating shaft 1202. The rotation of the first rotating shaft 1202 is driven by a drive mechanism.

[0030] The drive mechanism includes a protective cover 205 fixedly connected to the top of the fixed plate 1201, and a drive shaft 201 fixedly connected to the upper end of the first rotating shaft 1202. The side wall of the drive shaft 201 is provided with a sliding groove, and the sliding groove includes multiple V-shaped grooves 202 connected end to end. A push pin 204 is connected in the V-shaped groove 202 through a lifting mechanism. The side wall of the tube body 101 is provided with a limiting mechanism for limiting the drive shaft 201. The push pin 204 is driven to slide down along the sliding groove through the lifting mechanism. At this time, the drive shaft 201 can be driven to swing back and forth.

[0031] The lifting mechanism includes an L-shaped lifting rod 301 inserted into the top of the protective cover 205, and a fixing ring 302 is fixedly sleeved on the side wall of the tube body 101. The top of the fixing ring 302 is connected to a moving block 303 through a first reset mechanism, and a connecting rod 304 is fixedly connected to the bottom of the moving block 303. The lower end of the connecting rod 304 is fixed to the top of the lifting rod 301, and the movement of the moving block 303 is driven by a first pushing mechanism. When the filter screen 11 is clogged, the moving block 303 is pushed downward by the first pushing mechanism. When the moving block 303 moves downward, the pushing pin 204 can slide downward along the sliding groove through the connecting rod 304 and the lifting rod 301.

[0032] The first reset mechanism includes two symmetrically arranged sleeves 401 fixedly connected to the top of the fixed ring 302, and a sleeve rod 402 is inserted into the sleeve 401. The upper end of the sleeve rod 402 is fixed to the bottom of the moving block 303, and a first spring 403 is sleeved on the side wall of each sleeve 401, which guides and resets the movement of the moving block 303.

[0033] The first pushing mechanism includes a rectangular cover 501 fixedly connected to the top of the fixed ring 302, and a pushing block 502 slidably connected inside the rectangular cover 501. The pushing block 502 is fixed to the bottom of the moving block 303, and the rectangular cover 501 is connected to the tube body 101 through the connecting pipe 503. When the filter screen 11 is blocked, the air inside the rectangular cover 501 can be sucked out through the connecting pipe 503, so that a negative pressure is generated inside the rectangular cover 501. Under the action of the negative pressure, the pushing block 502 can slide down along the rectangular cover 501. At the same time, the first spring 403 is compressed.

[0034] The limiting mechanism includes multiple arrayed limiting holes 701 formed on the side wall of the drive shaft 201. The side wall of the tube body 101 is connected to a limiting rod 702 through a second reset mechanism. The movement of the limiting rod 702 is driven by a second pushing mechanism. During normal use, the limiting rod 702 is pushed into the limiting hole 701 by the second pushing mechanism to limit the drive shaft 201 and the first rotating shaft 1202.

[0035] The second reset mechanism includes a ring 801 fixedly sleeved on the side wall of the limiting rod 702. The limiting rod 702 extends into the tube body 101, and a second spring 802 is sleeved on the side wall of the limiting rod 702, which guides and resets the movement of the limiting rod 702.

[0036] The second driving mechanism includes a rotating plate 901 disposed above the filter screen 11, and the rotating plate 901 is rotatably connected to the top of the filter screen 11 through a rotating mechanism. Two symmetrically arranged first connecting blocks 903 are fixedly connected to the side wall of the rotating plate 901, and a connecting rod 902 is rotatably connected to the side wall of the first connecting block 903 through a first rotating pin 904. Two symmetrically arranged second connecting blocks 905 are fixedly connected to the end of the limiting rod 702, and the end of the connecting rod 902 away from the first connecting block 903 is rotatably connected to the side wall of the second connecting block 905 through a second rotating pin 906. During normal use, when the culture medium enters through the filter screen 11 and the inlet 104... When the liquid enters the tube 101, it impacts the surface of the rotating plate 901. Under the action of the liquid impact force, the rotating plate 901 can rotate clockwise along the rotating mechanism. At the same time, it drives the connecting rod 902 to rotate and pushes the limiting rod 702 to move and insert into the limiting hole 701. When the filter screen 11 is blocked by cell clumps, the impact force of the liquid on the rotating plate 901 is small. The rotating plate 901 will rotate counterclockwise along the second rotating shaft 1002. The limiting rod 702 can exit from the limiting hole 701 under the action of the second spring 802. At this time, the driving shaft 201 and the first rotating shaft 1202 are no longer limited.

[0037] The rotating mechanism includes two symmetrically arranged support blocks 1001 fixedly connected to the top of the filter screen 11, and the rotating plate 901 is rotatably connected to the side wall of the support block 1001 through the second rotating shaft 1002, ensuring the rotatable connection between the rotating plate 901 and the filter screen 11.

[0038] The bottom of the first rotating shaft 1202 and the scraper 1203 are provided with multiple mounting grooves 601, and the mounting grooves 601 are provided with ball bearings 602. Even when the scraper 1203 abuts against the bottom of the culture container, the ball bearings 602 can contact the bottom of the culture container first, ensuring that the first rotating shaft 1202 and the scraper 1203 can rotate normally.

[0039] Working principle: During use, when changing the medium during culture, first insert the tube body 101 into the culture medium. Then, flatten the rubber ball 102 and insert the conical tube 105 into the conical opening 103. Next, gradually loosen the rubber ball 102. At this time, a negative pressure is generated inside the tube body 101. At the same time, the culture medium is filtered through the filter screen 11 and enters the tube body 101 through the inlet 104. The filter screen 11 not only prevents cell clumps from clogging the pipette but also avoids the waste of cell clumps, ensuring the culture effect.

[0040] During normal use, when the culture medium enters the tube 101 through the filter screen 11 and the inlet 104, it impacts the surface of the rotating plate 901. Under the action of the liquid impact force, the rotating plate 901 can rotate clockwise along the second rotating shaft 1002. At the same time, it drives the connecting rod 902 to rotate and pushes the limiting rod 702 to move and insert into the limiting hole 701. At this time, the second spring 802 is compressed and can limit the drive shaft 201 and the first rotating shaft 1202.

[0041] When the filter screen 11 is clogged by cell clumps, the impact force of the liquid on the rotating plate 901 is small, and the rotating plate 901 will rotate counterclockwise along the second rotating shaft 1002. The limiting rod 702 can exit from the limiting hole 701 under the action of the second spring 802. At this time, the drive shaft 201 and the first rotating shaft 1202 are no longer limited.

[0042] Because the filter screen 11 is clogged, air can be drawn out from the rectangular cover 501 through the connecting pipe 503, creating a negative pressure inside the rectangular cover 501. Under the action of the negative pressure, the push block 502 can slide downward along the rectangular cover 501. At the same time, the first spring 403 is compressed. When the push block 502 moves downward, it can drive the moving block 303 to move downward. Furthermore, the push pin 204 can be driven to slide downward along the sliding groove through the connecting rod 304 and the lifting rod 301. At this time, the drive shaft 201 can be driven to swing back and forth. And the scraper 1203 can be driven to swing back and forth through the first rotating shaft 1202. At this time, the cell clumps at the bottom of the filter screen 11 can be automatically scraped and cleaned to prevent clogging and ensure the efficiency and effect of pipetting.

[0043] After cleaning, the limiting rod 702 can be inserted into the limiting hole 701 again for limiting. At this time, the drive shaft 201 no longer rotates. After the liquid is transferred, the conical tube 105 of the rubber ball 102 is pulled out from the conical opening 103. At this time, external air can enter the tube body 101, so that the tube body 101 and the rectangular cover 501 no longer form a negative pressure. The liquid can be discharged through the liquid inlet 104, so that the rotating plate 901 can rotate and reset. The limiting rod 702 can move and reset under the action of the second spring 802 and exit from the limiting hole 701. At the same time, the moving block 303 and the pushing block 502 can move upward and reset under the action of the first spring 403. Furthermore, the pushing pin 204 is driven to move upward and reset along the V-groove 202 through the connecting rod 304 and the lifting rod 301.

[0044] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0045] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A pipette for changing the medium during neural stem cell culture, comprising a tube body (101) and a rubber bulb (102), wherein the top of the tube body (101) is provided with a conical opening (103), the bottom of the tube body (101) is provided with a liquid inlet (104), and the bottom of the rubber bulb (102) is fixedly connected to a conical tube (105), characterized in that, A filter screen (11) is detachably connected inside the liquid inlet (104), and an installation groove (13) is provided inside the filter screen (11). A filter membrane (14) is inserted into the installation groove (13), and a cleaning mechanism for automatically cleaning the filter screen (11) is provided on the side wall of the tube body (101). The cleaning mechanism includes a fixed plate (1201) fixedly connected to the side wall of the pipe body (101), and a scraper (1203) is rotatably connected to the bottom of the fixed plate (1201) through a first rotating shaft (1202). The rotation of the first rotating shaft (1202) is driven by a driving mechanism.

2. The pipette for changing the medium during neural stem cell culture according to claim 1, characterized in that: The driving mechanism includes a protective cover (205) fixedly connected to the top of the fixed plate (1201), and a driving shaft (201) is fixedly connected to the upper end of the first rotating shaft (1202). The side wall of the driving shaft (201) is provided with a sliding groove, and the sliding groove includes a plurality of V-shaped grooves (202) connected end to end. A push pin (204) is connected in the V-shaped groove (202) through a lifting mechanism, and a limiting mechanism for limiting the driving shaft (201) is provided on the side wall of the tube body (101).

3. A pipette for changing the medium during neural stem cell culture according to claim 2, characterized in that: The lifting mechanism includes an L-shaped lifting rod (301) inserted into the top of the protective cover (205), and a fixing ring (302) is fixedly sleeved on the side wall of the tube body (101). The top of the fixing ring (302) is connected to a moving block (303) through a first reset mechanism, and a connecting rod (304) is fixedly connected to the bottom of the moving block (303). The lower end of the connecting rod (304) is fixed to the top of the lifting rod (301), and the movement of the moving block (303) is pushed by a first push mechanism.

4. A pipette for changing the medium during neural stem cell culture according to claim 3, characterized in that: The first reset mechanism includes two symmetrically arranged sleeves (401) fixedly connected to the top of the fixed ring (302), and a sleeve rod (402) is inserted inside the sleeve (401). The upper end of the sleeve rod (402) is fixed to the bottom of the moving block (303), and a first spring (403) is sleeved on the side wall of each sleeve (401).

5. A pipette for changing the medium during neural stem cell culture according to claim 3, characterized in that: The first pushing mechanism includes a rectangular cover (501) fixedly connected to the top of the fixed ring (302), and a pushing block (502) is slidably connected inside the rectangular cover (501). The pushing block (502) is fixed to the bottom of the moving block (303), and the rectangular cover (501) is connected to the tube body (101) through the connecting pipe (503).

6. A pipette for changing the medium during neural stem cell culture according to claim 2, characterized in that: The limiting mechanism includes multiple arrayed limiting holes (701) on the side wall of the drive shaft (201), and the side wall of the tube body (101) is connected to a limiting rod (702) through a second reset mechanism, and the movement of the limiting rod (702) is pushed by a second push mechanism.

7. A pipette for changing the medium during neural stem cell culture according to claim 6, characterized in that: The second reset mechanism includes a ring (801) fixedly sleeved on the side wall of the limiting rod (702), the limiting rod (702) extending into the tube body (101), and a second spring (802) sleeved on the side wall of the limiting rod (702).

8. A pipette for changing the medium during neural stem cell culture according to claim 6, characterized in that: The second pushing mechanism includes a rotating plate (901) disposed above the filter screen (11), and the rotating plate (901) is rotatably connected to the top of the filter screen (11) through a rotating mechanism. Two symmetrically arranged first connecting blocks (903) are fixedly connected to the side wall of the rotating plate (901), and a connecting rod (902) is rotatably connected to the side wall of the first connecting block (903) through a first rotating pin (904). Two symmetrically arranged second connecting blocks (905) are fixedly connected to the end of the limiting rod (702), and the end of the connecting rod (902) away from the first connecting block (903) is rotatably connected to the side wall of the second connecting block (905) through a second rotating pin (906).

9. A pipette for changing the medium during neural stem cell culture according to claim 8, characterized in that: The rotating mechanism includes two symmetrically arranged support blocks (1001) fixedly connected to the top of the filter screen (11), and the rotating plate (901) is rotatably connected to the side wall of the support block (1001) through the second rotating shaft (1002).

10. A pipette for changing the medium during neural stem cell culture according to claim 1, characterized in that: The bottom of the first rotating shaft (1202) and the scraper (1203) are provided with multiple mounting grooves (601), and ball bearings (602) are provided in the mounting grooves (601).

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