A rapid detection of probiotic activity reagent device and equipment

Abstract

The present application relates to the field of probiotic activity detection, in particular to a reagent device for rapid detection of probiotic activity and equipment, comprising a shaking cup and a shaking device; the reagent device further comprises: a separation ring coaxially arranged in the shaking cup along the axis of the shaking cup, the diameter of the separation ring is the same as the diameter of the shaking cup, and the separation ring is made of a deformable material; a sampling device arranged at the upper part of the shaking cup; a storage cup arranged in a straight line with the shaking cup, and a plurality of storage cups are used to store reagents and probiotic samples respectively; a first linear driver for driving the sampling device to reciprocate along the arrangement direction of the storage cup; and an ultraviolet spectrophotometer arranged on one side of the storage cup and located on the moving path of the sampling device. The present application avoids the scattering and shielding interference of foam on the light path, and improves the reliability of the data.

Description

Technical Field

[0001] This invention relates to the field of probiotic activity detection, specifically to a rapid probiotic activity detection reagent device and equipment. Background Technology

[0002] Since some probiotics have specific cleaning functions, in existing technologies, some probiotics are added to cleaning agents or detergents to enhance the cleaning effect. At the same time, in order to verify the effect, it is usually necessary to test the activity of probiotics (the number of live bacteria in the cleaning agent or detergent).

[0003] Chinese Patent Publication No. CN113670845B discloses a rapid detection method for probiotic activity using a kit. The method includes the following steps: S1: Take probiotic culture into a sterile centrifuge tube, add Polygonatum sibiricum polysaccharide extract, centrifuge, and discard the supernatant; S2: Under sterile conditions, add PBS buffer to the culture from S1 and mix thoroughly, resuspend the bacterial precipitate, and vortex until a sterile block is formed; S3: Add MTT staining solution to the mixture from S2 and mix thoroughly; S4: Place the mixture from S3 in a light-protected incubator for reaction, centrifuge after reaction, and discard the supernatant; S5: Add dimethyl sulfoxide to the reaction product after centrifugation from S4, vortex until a sterile block is formed, incubate at room temperature in the dark for 8-12 minutes, centrifuge, discard the precipitate, and collect the supernatant; S6: Place the supernatant from S5 in a UV spectrophotometer, measure its absorbance at 545 nm, and calculate the viable cell count based on the absorbance value.

[0004] The above-mentioned method can extract and detect single bacterial solutions. However, when detecting liquid samples containing probiotics, such as detergents and cleaning agents (e.g., probiotic laundry detergent, spray cleaner), the shaking process during detection generates a large amount of foam. Existing detection devices lack anti-foaming design, and when sampling the shaken bacterial solution, a large amount of foam enters the sampler's pipes. This foam is difficult to eliminate. If samples containing foam are injected into the UV spectrophotometer, the foam significantly affects the detection results. Furthermore, the current operation relies heavily on manual operation by staff, resulting in a large workload. Strict adherence to experimental procedures is required, and prolonged work can lead to fatigue and inaccurate results. Summary of the Invention

[0005] To address the aforementioned issues, a rapid probiotic activity detection reagent device and equipment are provided. By integrating a first linear actuator, sampling device, oscillation device, and ultraviolet spectrophotometer into a linked structure, the entire process of probiotic activity detection—sampling, oscillation, transport, and detection—is automated. This eliminates the need for traditional manual operation, significantly reducing the workload of laboratory personnel, alleviating fatigue from prolonged repetitive operations, and fundamentally reducing human errors such as sample addition deviations and omissions. Simultaneously, relying on the foam partition design of the deformable separation ring, after oscillation and settling, the separation ring deforms, gathering the foam within the ring's inner area, creating a foam-free, pure sampling area outside the ring. The sampling device only takes samples and transports them within the outer ring, without inhaling or contaminating foam throughout the entire process, completely avoiding foam scattering and obstruction interference on the light path.

[0006] To address the problems of existing technologies, this invention provides a rapid detection reagent device for probiotic activity, comprising a shaking cup and a shaking device;

[0007] The reagent device also includes: A separation ring is coaxially disposed in the oscillating cup along the axis of the oscillating cup. The diameter of the separation ring is the same as the diameter of the oscillating cup. The separation ring is made of a deformable material. After the separation ring is deformed under the action of external force, it can divide the oscillating cup into an inner ring area and an outer ring area. The inner ring area is where the foam generated by oscillation accumulates. A sampling device is disposed at the upper part of the vibrating cup; Multiple storage cups are provided and arranged in a straight line with the shaking cup, and the multiple storage cups are used to store reagents and probiotic samples respectively; A first linear actuator is used to drive the sampling device to reciprocate along the arrangement direction of the storage cups; An ultraviolet spectrophotometer is disposed on one side of the storage cup and located on the moving path of the sampling device.

[0008] Preferably, a lever extending into the interior of the oscillating cup is vertically disposed in the oscillating cup, and the lever is connected to the separation ring.

[0009] Preferably, the separating ring has a slot for inserting the lever.

[0010] Preferably, a limiting plug for limiting the bottom of the separating ring is inserted into the lower part of the lever.

[0011] Preferably, the sampling device includes: A follower rod, vertically positioned, is used to push the lever to move; The sampler is located on the side of the follower rod away from the oscillating cup; A second linear actuator is disposed on the upper part of the sampler and is used to drive the sampler to move vertically.

[0012] Preferably, a guide frame is horizontally fixed on the upper part of the lever, and a guide rail is provided on one side of the oscillating cup parallel to the arrangement direction of the storage cup. A guide block is provided in the guide rail that is inserted and engaged with the lower part of the guide frame.

[0013] Preferably, a spring is provided inside the guide rail along the extension direction of the guide rail, and the two ends of the spring are respectively connected to the guide block and the end of the guide rail.

[0014] Preferably, the oscillation unit includes: A support is provided around the vibrating cup to support it. The swing mechanism is mounted on the support frame; A rotary driver is used to drive the swing mechanism to operate, and the rotary driver drives the support to swing back and forth through the swing mechanism.

[0015] Preferably, a limiting mechanism for limiting the rotation of the oscillating cup is provided on the support.

[0016] The present invention also relates to a rapid detection reagent device for probiotic activity, comprising a housing, an operation screen, and a rapid detection reagent device for probiotic activity.

[0017] The advantages of this invention compared to the prior art are: 1. This invention, by integrating a first linear actuator, sampling device, oscillation device, and ultraviolet spectrophotometer into a linked structure, automates the entire process of probiotic activity detection, including sampling, oscillation, transport, and detection. This eliminates the need for traditional manual operation, significantly reducing the workload of laboratory personnel, alleviating fatigue from prolonged repetitive operations, and fundamentally reducing human errors such as sample addition deviations and omissions. Simultaneously, relying on the foam partition design of the deformable separation ring, after oscillation and settling, the separation ring deforms, gathering the foam within the ring and creating a foam-free, pure sampling area outside the ring. The sampling device only takes samples and transports them in the outer ring area, without inhaling or contaminating foam throughout the process. This completely avoids the scattering and obstruction of light by foam, ensuring accurate absorbance readings, accurate calculation of viable bacteria counts, and compatibility with clean samples containing surfactants, thus improving data reliability.

[0018] 2. Through the coordinated structural design of the lever, limit plug, guide rail, and spring, precise control, stable limiting, and automatic reset of the deformable separation ring are achieved, significantly optimizing the ease of operation and cost-effectiveness of the device. The lever, in conjunction with the slot, allows for flexible installation and removal of the separation ring. The bottom limit plug effectively prevents the separation ring from sliding or shifting during oscillation, ensuring it remains at the liquid surface for stable foam interception. Separation effectiveness is guaranteed without additional materials, reducing consumable costs. The separation ring can be either a reusable silicone type or a disposable paper type, balancing economy and hygiene. The guide rail and spring enable automatic lever reset, eliminating manual operation. The guide frame and guide block ensure smooth lever movement, preventing the separation ring from shifting, further improving foam separation reliability and extending the device's service life.

[0019] 3. The device achieves a dual improvement in operational stability and testing safety through a shaking cup limiting mechanism, a sampler self-cleaning system, and modular design. The limiting mechanism on the support prevents the shaking cup from rotating during shaking, preventing damage to the separation ring and ensuring stable operation of the shaking and foam separation steps. The sampler features a self-cleaning mechanism and a matching wastewater tank, automatically cleaning and draining wastewater after each sampling to prevent cross-contamination between different samples and reagents, ensuring pure and reliable test data. The entire device, equipped with an external operating panel, allows for preset test parameters, adapting to standardized batch sample testing. It perfectly meets the high-volume quality inspection needs of probiotic cleaning products in the daily chemical industry. The compact layout facilitates maintenance, comprehensively improving testing efficiency and meeting the requirements for high-precision and high-stability testing. Attached Figure Description

[0020] Figure 1 This is a three-dimensional schematic diagram of a rapid probiotic activity detection reagent device according to the present invention.

[0021] Figure 2 This is a partial cross-sectional three-dimensional schematic diagram of a rapid probiotic activity detection reagent device of the present invention.

[0022] Figure 3 This invention relates to a rapid detection reagent device for probiotic activity. Figure 2 A magnified view of a portion of point A in the middle.

[0023] Figure 4 This invention relates to a rapid detection reagent device for probiotic activity. Figure 2 A magnified view of a portion of point B in the middle.

[0024] Figure 5 This is a three-dimensional schematic diagram of the rapid probiotic activity detection reagent device of the present invention after the outer shell has been removed.

[0025] Figure 6 This invention relates to a rapid detection reagent device for probiotic activity. Figure 5 A magnified view of a portion of point C.

[0026] Figure 7 This is a three-dimensional schematic diagram of the device for rapid detection of probiotic activity reagents according to the present invention, after removing the storage cup and the first linear actuator.

[0027] Figure 8 This is a three-dimensional schematic diagram of the lever being removed from the separation ring in the rapid probiotic activity detection reagent device of the present invention.

[0028] Figure 9 This is a three-dimensional schematic diagram of the lever with a separation ring in the rapid probiotic activity detection reagent device of the present invention being removed from the shaking cup together.

[0029] Figure 10 This invention relates to a rapid detection reagent device for probiotic activity. Figure 9 A magnified view of a portion of point D.

[0030] Figure 11 This is a cross-sectional three-dimensional schematic diagram of the shaking cup in the rapid probiotic activity detection reagent device of the present invention.

[0031] Figure 12 This is a three-dimensional schematic diagram of the shaking cup being removed from the support in the rapid probiotic activity detection reagent device of the present invention.

[0032] The following are the labels in the diagram: 1. Shaking cup; 11. Separating ring; 111. Slot; 12. Lever; 13. Limiting plug; 14. Guide frame; 15. Guide rail; 151. Guide block; 152. Spring; 2. Shaking device; 21. Support; 22. Swinging mechanism; 221. Base; 222. Extension groove; 223. Rotating disk; 23. Rotary actuator; 24. Limiting mechanism; 241. Limiting groove; 242. Limiting block; 3. Sampling device; 31. Follower rod; 32. Sampler; 321. Inlet and outlet; 322. Liquid inlet; 33. Second linear actuator; 34. Follower frame; 4. Storage cup; 5. First linear actuator; 6. Ultraviolet spectrophotometer; 7. Housing; 71. Sewage tank; 8. Operation panel. Detailed Implementation

[0033] To further understand the features, technical means, and specific objectives and functions achieved by the present invention, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.

[0034] Reference Figures 1 to 3 A rapid detection reagent device for probiotic activity includes a shaking cup 1 and a shaking device 2; The reagent device also includes: A separation ring 11 is coaxially disposed in the oscillating cup 1 along the axis of the oscillating cup 1. The diameter of the separation ring 11 is the same as the diameter of the oscillating cup 1. The separation ring 11 is made of a deformable material. After the separation ring 11 is deformed under the action of external force, it can divide the oscillating cup 1 into an inner ring area and an outer ring area. The inner ring area is where the foam generated by oscillation accumulates. Sampling device 3 is disposed on the upper part of the vibrating cup 1; Storage cups 4 are provided in multiples and arranged in a straight line with the shaking cup 1. The multiple storage cups 4 are used to store reagents and probiotic samples respectively. The first linear actuator 5 is used to drive the sampling device 3 to reciprocate along the arrangement direction of the storage cup 4; The ultraviolet spectrophotometer 6 is disposed on one side of the storage cup 4 and located on the moving path of the sampling device 3.

[0035] The above text mentions that the separation ring is made of a deformable material, which includes materials that can deform under compression. Before oscillation, the separation ring 11 is in a ring-shaped state, and the outer circumferential sidewall of the separation ring 11 is in contact with the inner wall of the oscillation cup 1. After oscillation and settling, one side of the separation ring 11 is pushed, causing the separation ring 11 to deform. The foam generated after oscillation is located in the separation ring 11. Because the foam has a large buoyancy, it will float above the liquid. When the separation ring 11 deforms, the foam is always located inside the ring of the separation ring 11. After the separation ring 11 deforms, an inner ring area and an outer ring area are formed in the oscillation cup 1. The foam is located in the inner ring area, and no foam floats in the outer ring area. The sampling device 3 takes samples in the outer ring area during sampling and transportation.

[0036] When testing liquid samples of cleaning agents and detergents containing probiotics, such as probiotic laundry detergent and spray cleaners, the shaking and vortexing operations required during testing generate a large amount of fine foam due to the violent disturbance. Existing testing devices generally lack specific anti-foaming structures, failing to effectively suppress foam generation or promptly eliminate existing foam. During automatic or manual sampling of the shaken bacterial solution, a large amount of foam flows into the sampler's pipes, occupying pipe space and adhering to the pipe walls, making it difficult to eliminate quickly. This directly leads to inaccurate sampling volume and poor liquid delivery. If samples containing a large amount of foam are directly injected into a UV spectrophotometer, the foam scatters, refracts, and blocks light, severely interfering with the accurate reading of absorbance values. This results in overly high or low test results, or extremely poor repeatability, failing to accurately reflect the actual number of live probiotics in the sample.

[0037] Furthermore, the entire testing process relies heavily on manual operation by laboratory personnel. From sampling, adding reagents, shaking, and incubating in the dark to centrifugation, reading, and calculation, the steps are cumbersome and interconnected, requiring extremely high levels of operational standardization and consistency. In batch sample testing scenarios, the workload for staff is high, involving numerous repetitive operations. Prolonged continuous work can easily lead to visual fatigue and operational errors, such as deviations in sample volume, inaccurate control of reaction time, and improper control of light-shielding conditions. These human errors further amplify the instability of the test results, reducing testing efficiency and failing to meet the actual needs of large-scale, standardized, and high-precision probiotic activity testing in daily chemical cleaning products.

[0038] To avoid the aforementioned issues, this invention optimizes the design of existing probiotic active reagent devices, automating sampling, shaking, transport, and detection. This reduces the workload, and during transport after shaking, the sampling device 3 does not inhale foam, ensuring the accuracy of the test results. The specific structure and working principle of this invention are as follows: The first linear actuator 5 is preferably a linear slide. Before detection, the reagents needed for the detection process and the sample containing probiotics are injected into different storage cups 4, and then the storage cups 4 are placed sequentially into the reagent device, with all storage cups 4 arranged in a straight line with the shaking cup 1. The device is then started, and the first linear actuator 5 drives the sampling device 3 to move. The sampling device 3 sequentially takes samples from different storage cups 4 and injects them into the shaking cup 1. It is worth noting that after each sampling, the sampling device 3 needs to perform a self-cleaning process to avoid cross-contamination of the liquids in different storage cups 4. After the sampling device 3 completes the injection, the shaking device 2 drives the shaking cup 1 to shake, and the shaking device 2 shakes the shaking cup 1 according to a preset frequency and time.

[0039] It is worth noting that during oscillation, the separating ring 11 has a ring structure, and the outer wall of the separating ring 11 is attached to the inner wall of the oscillating cup 1. After oscillation is completed and the mixture is allowed to stand, one side of the separating ring 11 attached to the inner wall of the oscillating cup 1 is pushed, causing the separating ring 11 to deform. The deformed separating ring 11 gathers the foam inside the ring and forms an inner ring area and an outer ring area in the oscillating cup 1. The foam is located in the inner ring area, and there is no foam floating above the liquid surface in the outer ring area. Subsequently, the sampling device 3 moves to the upper part of the outer ring area in the oscillating cup 1 under the drive of the first linear driver 5 and samples the liquid in the outer ring area. Because there is no foam above the liquid in the outer ring area, the sampling device 3 will not suck in foam or have foam adhering to the outside of the sampling device 3 during the sampling process. This ensures that when the sampling device 3 brings the oscillated sample to the ultraviolet spectrophotometer 6 for discharge, there will be no foam interference in the sample in the ultraviolet spectrophotometer 6, thus ensuring the accuracy of the detection results.

[0040] Reference Figure 9 and Figure 10 A lever 12 extending into the interior of the oscillating cup 1 is vertically disposed in the oscillating cup 1, and the lever 12 is connected to the separation ring 11.

[0041] After the oscillating cup 1 completes oscillation, the lever 12 is used to move one side of the separation ring 11, causing the separation ring 11 to deform and form an inner ring area and an outer ring area in the oscillating cup 1, which facilitates sampling by the subsequent sampling device 3.

[0042] Reference Figure 8 The separation ring 11 has a slot 111 for inserting the lever 12.

[0043] By setting a slot 111 on the separation ring 11, it is ensured that the lever 12 can be movably connected with the separation ring 11. After completing one test, the staff can remove the lever 12 and the separation ring 11 and replace the used separation ring 11. If the separation ring 11 is made of silicone or other materials, it can be reused after being cleaned and dried, reducing the testing cost. In addition, if it is inconvenient to clean, a paper separation ring 11 can also be used, which can be discarded after use, and the usage cost is still low.

[0044] Reference Figure 10 A limiting plug 13 for limiting the bottom of the separating ring 11 is inserted into the lower part of the lever 12.

[0045] Since the lever 12 is inserted into the slot 111 of the separating ring 11, if no limiting plug 13 is provided at the bottom of the lever 12, when the lever 12 is inserted into the slot 111 of the separating ring 11, the separating ring 11 will gradually slide towards the bottom of the shaking cup 1 during the shaking process. If the vertical height of the separating ring 11 is limited, the upper part of the separating ring 11 will sink below the liquid surface in the shaking cup 1, causing the separating ring 11 to fail to separate the foam. To overcome the above situation, two methods are mainly adopted: First, increase the height of the separating ring 11 so that the bottom of the separating ring 11... Even when the part of the separating ring 11 contacts the bottom of the shaking cup 1, the upper part of the separating ring 11 remains above the liquid surface, still effectively intercepting foam. However, this increases the material consumption per separating ring 11, raising the operating cost. Secondly, a limiting plug 13 is installed at the bottom of the lever 12. This limiting plug 13 restricts the separation ring 11, ensuring that, with less material consumption, the separating ring 11 remains at the liquid surface—that is, the upper part of the separating ring 11 is above the liquid surface, and the lower part is below—ensuring stable foam separation. In long-term use, method two is more economical.

[0046] Reference Figure 5 and Figure 6 The sampling device 3 includes: The follower rod 31 is vertically arranged and used to push the lever 12 to move; The sampler 32 is located on the side of the follower rod 31 away from the oscillating cup 1; The second linear actuator 33 is disposed on the upper part of the sampler 32 and is used to drive the sampler 32 to move in the vertical direction.

[0047] The second linear actuator 33 is preferably a servo electric cylinder. The lower end of the follower rod 31 is at a lower horizontal height than the upper end of the lever 12. When the first linear actuator 5 drives the sampler 32 to move, the follower rod 31 first contacts the lever 12. After the follower rod 31 pushes the lever 12, the sampler 32 moves to the top of the oscillating cup 1. At this time, the outer ring area in the oscillating cup 1 has been formed. The sampler 32 descends and performs sampling under the drive of the second linear actuator 33. The sampler 32 is provided with an inlet and outlet port 321, and a pump body is connected to the inlet and outlet port 321. After the lower end of the sampler is inserted below the liquid surface in the shaking cup 1, the pump is activated to expel the air from the sampler 32. The sampler 32 then draws in the liquid from the shaking cup 1, thus completing the sampling. Subsequently, the second linear actuator 33 drives the sampler 32 to rise, and the first linear actuator 5 drives the sampler 32 to move above the UV spectrophotometer 6. The first linear actuator 5 then drives the sampler 32 to descend, and the pump supplies air to the sampler 32. The sample remaining in the sampler 32 is then injected into the UV spectrophotometer 6 under the action of the pump.

[0048] The sampling device 3 also includes a follower frame 34, on which the follower rod 31 and the sampler 32 are both mounted. The follower frame 34 is directly connected to the first linear driver 5, which drives the follower frame 34 to move along the arrangement direction of the storage cups 4.

[0049] Reference Figure 7 A guide frame 14 is horizontally fixed on the upper part of the lever 12. A guide rail 15 is arranged on one side of the oscillating cup 1 parallel to the arrangement direction of the storage cup 4. A guide block 151 is arranged inside the guide rail 15 and is inserted into the lower part of the guide frame 14.

[0050] By setting up the guide frame 14, guide rail 15, and guide block 151, the stability of the movement direction of the lever 12 is ensured when it is pushed. When replacing the separation ring 11, first remove the guide frame 14 from the guide block 151. The lever 12 is removed along with the guide frame 14. Since the limiting plug 13 at the bottom of the lever 12 is not removed at this time, the separation ring 11 fitted on the lever 12 is removed at the same time as the lever 12. Then, remove the limiting plug 13 at the bottom of the lever 12 and remove the separation ring 11 for replacement. It is worth noting that the vibrating cup 1 needs to be manually cleaned after each use. Therefore, after removing the guide frame 14, the vibrating cup 1 needs to be removed and cleaned.

[0051] Reference Figure 7 A spring 152 is provided inside the guide rail 15 along the extension direction of the guide rail 15, and the two ends of the spring 152 are respectively connected to the guide block 151 and the end of the guide rail 15.

[0052] When the follower rod 31 in the sampling device 3 pushes the lever 12, the spring 152 in the guide rail 15 is pushed and squeezed by the guide block 151. After sampling is completed, when the first linear driver 5 drives the sampling device 3 to leave the oscillating cup 1, the spring 152 pushes the guide block 151 under the action of the reset elastic force, so that the guide block 151 drives the lever 12 to reset through the guide frame 14.

[0053] Reference Figures 7 to 9 The oscillation unit includes: The support 21 is disposed around the vibrating cup 1 and supports the vibrating cup 1; The swing mechanism 22 is mounted on the bracket 21; The rotary driver 23 is used to drive the swing mechanism 22 to operate, and the rotary driver 23 drives the bracket 21 to swing back and forth through the swing mechanism 22.

[0054] The rotary actuator 23 is preferably a servo motor. In the prior art, there are many structures for the swing mechanism 22; this section only lists one embodiment. The swing mechanism 22 includes a base 221, an extension groove 222, a rotating disk 223, and an extension column. The base 221 is disposed at the lower part of the support 21 and hinged to the support 21. The hinge point between the support 21 and the base 221 is called the hinge point. An extension groove 222 is vertically disposed below the hinge point. The rotating disk 223 is rotatably disposed on one side of the extension groove 222. The extension column is fixedly disposed on the rotating disk 223 and extends into the extension groove 222. The extension column and the extension groove 222 are in sliding engagement. The axis of the extension column is not collinear with the axis of the rotating disk 223. The output end of the rotary actuator 23 is fixedly connected to the end of the rotating disk 223. When the rotary actuator 23 drives the rotating disk 223 to rotate, the rotating disk 223 slides in the extension groove 222 via the extension column, causing the support 21 to swing on the base 221.

[0055] Reference Figures 1 to 12 A limiting mechanism 24 for limiting the rotation of the oscillating cup 1 is provided on the bracket 21.

[0056] During the shaking process of the oscillating device 2 on the oscillating cup 1, in order to prevent the oscillating cup 1 from rotating on the support 21 due to shaking, which would cause the rotating oscillating cup 1 to exert a pulling force on the separating ring 11, especially when the separating ring 11 is made of paper material, the pulling force generated by the rotating oscillating cup 1 can easily cause the separating ring 11 to break. To avoid the above situation, a limiting mechanism 24 is provided. The limiting mechanism 24 includes a limiting groove 241 provided on the support 21 and a limiting block 242 provided on the oscillating cup 1. The limiting block 242 and the limiting groove 241 are inserted and engaged.

[0057] Reference Figures 1 to 12 The present invention also relates to a rapid detection reagent device for probiotic activity, comprising a housing 7, an operation screen 8, and a rapid detection reagent device for probiotic activity.

[0058] The operation panel 8 is located on the outside of the housing 7. The operator can preset test parameters through the operation panel 8, such as the sampling amount of the sampling device 3, the oscillation time and frequency of the oscillation device 2, etc.

[0059] The sampler 32 is also equipped with an inlet 322 for discharging cleaning fluid into the sampler 32. A drain tank 71 is also provided in the outer casing 7, arranged in a straight line with the shaking cup 1 and the storage cup 4. After each sampling, the sampling device 3 needs to move above the drain tank 71, then the inlet 322 opens to discharge the cleaning fluid into the sampler 32. A reversing valve is provided at the inlet 322; after a measured amount of cleaning fluid is discharged, the reversing valve reverses, injecting clean water into the sampler 32 for further rinsing. The wastewater discharged during cleaning is discharged into the drain tank 71. A valve body is provided at the bottom of the drain tank 71; the valve body opens periodically to discharge the wastewater stored in the drain tank 71.

[0060] The above embodiments only illustrate one or more implementations of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of protection of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the appended claims.

Claims

1. A rapid detection reagent device for probiotic activity, comprising a shaking cup (1) and a shaking device (2); Its features are, The reagent device also includes: A separation ring (11) is coaxially disposed in the oscillating cup (1) along the axis of the oscillating cup (1). The diameter of the separation ring (11) is the same as the diameter of the oscillating cup (1). The separation ring (11) is made of a deformable material. After the separation ring (11) is deformed under the action of external force, it can divide the oscillating cup (1) into an inner ring area and an outer ring area. The inner ring area is where foam generated by oscillation accumulates. A sampling device (3) is disposed on the upper part of the vibrating cup (1); Storage cups (4) are provided in multiples and arranged in a straight line with the shaking cup (1). The multiple storage cups (4) are used to store reagents and probiotic samples respectively. The first linear actuator (5) is used to drive the sampling device (3) to reciprocate along the arrangement direction of the storage cup (4); The ultraviolet spectrophotometer (6) is set on one side of the storage cup (4) and located on the moving path of the sampling device (3).

2. The rapid detection reagent device for probiotic activity according to claim 1, characterized in that, A lever (12) extending into the interior of the oscillating cup (1) is vertically arranged in the oscillating cup (1), and the lever (12) is connected to the separation ring (11).

3. The rapid detection reagent device for probiotic activity according to claim 2, characterized in that, The separation ring (11) has a slot (111) for inserting the lever (12).

4. The rapid detection reagent device for probiotic activity according to claim 3, characterized in that, The lower part of the lever (12) is fitted with a limiting plug (13) for limiting the bottom of the separating ring (11).

5. A rapid detection reagent device for probiotic activity according to any one of claims 2-4, characterized in that, The sampling device (3) includes: Follower rod (31) is vertically arranged and used to push the lever (12) to move; The sampler (32) is located on the side of the follower rod (31) away from the oscillating cup (1); A second linear actuator (33) is disposed on the upper part of the sampler (32) for driving the sampler (32) to move in the vertical direction.

6. The rapid detection reagent device for probiotic activity according to claim 2, characterized in that, A guide frame (14) is fixedly mounted horizontally on the upper part of the lever (12). A guide rail (15) is provided on one side of the oscillating cup (1) parallel to the arrangement direction of the storage cup (4). A guide block (151) is provided inside the guide rail (15) and is inserted into the lower part of the guide frame (14).

7. The rapid detection reagent device for probiotic activity according to claim 6, characterized in that, A spring (152) is provided inside the guide rail (15) along the extension direction of the guide rail (15), and the two ends of the spring (152) are respectively connected to the guide block (151) and the end of the guide rail (15).

8. The rapid detection reagent device for probiotic activity according to claim 6, characterized in that, The oscillation unit includes: A support (21) is provided around the vibrating cup (1) and supports the vibrating cup (1); A swing mechanism (22) is mounted on a bracket (21); A rotary driver (23) is used to drive the swing mechanism (22) to operate. The rotary driver (23) drives the support (21) to swing back and forth through the swing mechanism (22).

9. The rapid detection reagent device for probiotic activity according to claim 8, characterized in that, A limiting mechanism (24) for limiting the rotation of the oscillating cup (1) is provided on the bracket (21).

10. A rapid detection reagent device for probiotic activity, characterized in that, It includes a housing (7), an operation screen (8), and a rapid detection reagent device for probiotic activity as described in any one of claims 1-9.

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