Plasma-activated nano-silver gel, preparation method and application thereof

Abstract

This invention discloses a plasma-activated silver nanogel, its preparation method, and its application. The preparation method includes the following steps: S1, mixing silver nanoparticles with agarose solution, heating and reacting, and then allowing it to stand until it becomes a gel; S2, immersing the gel obtained in S1 in physiological saline; S3, subjecting the gel treated in S2 to plasma treatment to obtain the plasma-activated silver nanogel. The method of this invention locks the active particles in the gel before applying it to the skin surface, avoiding direct or close-range contact between the plasma generator and the skin, thus preventing skin burns and ensuring safety. Simultaneously, it synergistically enhances the effects of silver ions and ClO₂. ‑ It has a dual antibacterial mechanism, which relieves redness, swelling, and thickened scales, accelerates the resolution of inflammation, regulates the immune response, and relieves symptoms of chronic inflammatory diseases (such as psoriasis).

Description

Technical Field

[0001] This invention relates to the field of plasma biomedical application technology, specifically to a plasma-activated silver nanogel, its preparation method, and its application. Background Technology

[0002] Plasma is the fourth state of matter besides solid, liquid, and gas. Low-temperature plasma generated under atmospheric pressure contains various reactive particles, which possess biomedical effects such as sterilization, anti-cancer properties, and wound-healing promotion. Plasma has been widely used in the medical and cosmetic fields. When plasma acts on the skin surface, it can promote the repair of the skin barrier and has a certain alleviating effect on the symptoms of skin diseases such as psoriasis, vitiligo, and atopic dermatitis.

[0003] However, if plasma is applied directly to the skin surface, although it may provide relief, the plasma has the characteristics of sharp discharge and strong reactivity, which may pose a safety risk of skin burns from electrode heating. Moreover, the depth of effect varies and the stability cannot be guaranteed.

[0004] Chinese patent application CN119868402 A discloses a plasma activation solution, its preparation method, and its application. This application uses liquid physiological saline (containing reactive nitrogen), and the active ingredient has a short half-life (<1 hour), requiring frequent spraying. The active particle component is mainly reactive nitrogen (RNS). Chinese patent application CN119015218 A discloses a gel, a plasma-locking gel, and its application. The gel, after plasma treatment, can lock in single active particles (NO2) to a certain extent. - (H2O2 / O3), and the active particles are released through temperature-sensitive pores, so the transdermal efficiency is limited by the ambient temperature. Summary of the Invention

[0005] The technical problem to be solved by this invention is how to reduce the harm caused by plasma directly acting on the skin surface and improve the shortcomings of active particles, such as weak penetration, rapid decay and insignificant effect.

[0006] The present invention solves the above-mentioned technical problems through the following technical means: A method for preparing plasma-activated silver nanogel includes the following steps: S1. Mix silver nanoflower particles with agarose solution, heat to react, and let stand until it becomes gel-like. S2. Soak the gelatinous substance obtained in S1 in physiological saline; S3. The gel-like substance treated in S2 is subjected to plasma treatment to obtain the plasma-activated nano-silver gel.

[0007] Preferably, in S1, the size of the silver nanoflower particles is 500 nm.

[0008] Preferably, in S1, the preparation method of the silver nanoflower particles includes the following steps: mixing silver nitrate aqueous solution, polyvinylpyrrolidone aqueous solution and water evenly, adding ascorbic acid aqueous solution to obtain a reaction solution, and stirring the reaction solution to obtain the silver nanoflower particles.

[0009] Preferably, the molar ratio of silver nitrate, polyvinylpyrrolidone, and ascorbic acid in the reaction solution is 0.02:1-1.5:0.2.

[0010] Preferably, in S1, the mass fraction of the agarose solution is 1%; the mass-to-volume ratio of silver nanoflower particles to agarose solution is 2-3 g:50 mL.

[0011] Preferably, the agarose solution is an aqueous agarose solution.

[0012] Preferably, in step S1, the silver nanoflower particle solution is mixed with the agarose solution; the silver nanoflower particle solution is an ethanol solution of silver nanoflower particles, and the concentration of the silver nanoflower particles is 2-3 g / mL, preferably 2.1574 g / mL.

[0013] Preferably, in S1, the reaction temperature is 85-95 °C and the time is 5-15 min.

[0014] Preferably, in S2, the mass concentration of the physiological saline is 0.8-1.2 wt%.

[0015] Preferably, in S3, during the plasma treatment process, the distance between the plasma source and the gel-like substance to be treated is 1-1.5 cm, the voltage is 42-50 kV, the power is 2-5 watts, and the treatment time is 2-3 min.

[0016] Preferably, in S1, after heating and reacting, the mixture is placed in a mold and left to stand until it becomes a gel.

[0017] Preferably, the preparation method of the plasma-activated silver nanogel includes the following steps: at room temperature, 0.1M silver nitrate aqueous solution and 1M polyvinylpyrrolidone aqueous solution are added to water and magnetically stirred until the mixture is fully homogeneous. Then, 1M ascorbic acid aqueous solution is injected into the stirred mixture and reacted for 10-15 min to prepare colloidal silver nanoflower particles with a size of 500 nm; the silver nanoflower particles are added to a 1% (w / w) thickener agarose solution and heated at 90°C for 10 min, and allowed to stand until it becomes gel-like to obtain a gel; the gel is immersed in physiological saline, taken out and treated in a plasma generator to generate active particles and encapsulate them in a hydrogel to obtain the plasma-activated silver nanogel.

[0018] Preferably, the active particles include a variety of active substances such as active chlorine, active oxygen, and active nitrogen.

[0019] The method of the present invention locks the active particles in the gel and then applies them to the skin surface, which can avoid direct or close-range contact between the plasma generator and the skin surface, thereby avoiding skin burns and ensuring safety.

[0020] The present invention also proposes a plasma-activated silver nanogel, which is prepared by the aforementioned method for preparing plasma-activated silver nanogel.

[0021] This invention also proposes the application of plasma-activated nano-silver gel in the preparation of patches for treating psoriasis.

[0022] Preferably, the prepared patch for treating psoriasis can reduce the levels of pro-inflammatory factors IL-17, IL-23, TNF-α, and IL-22 in the body.

[0023] Preferably, the prepared patch for treating psoriasis is prepared by moistening the target skin area with physiological saline before use.

[0024] The present invention also proposes a gel patch containing the aforementioned plasma-activated nano-silver gel.

[0025] Preferably, the gel patch is a gel patch that can relieve symptoms of chronic inflammatory diseases.

[0026] To reduce the harm caused by direct plasma action on the skin surface and avoid the drawbacks of weak penetration, rapid attenuation, and insignificant effects of active particles, this invention prepares a gel patch containing silver nanoparticles. After plasma treatment, the patch can be applied to the inflamed skin surface. Furthermore, we designed a method to soak the gel patch in physiological saline. This plasma activation treatment softens the stratum corneum, reduces barrier resistance, and mitigates the damage caused by direct plasma treatment. The three-dimensional network structure of the gel also provides a long-term retention effect on the active particles generated by the plasma. Simultaneously, it innovatively combines plasma activation with the generation of ClO2 in the physiological saline. - With nano-silver sustained-release Ag + It forms a dual-active system, which has a good effect on inhibiting skin inflammation and antibacterial and anti-inflammatory effects.

[0027] The advantages of this invention are: 1. The silver nano-flower particles introduced into the gel of this invention have multiple significant advantages, mainly reflected in antibacterial, anti-inflammatory and immunomodulatory effects.

[0028] 2. In the gel of this invention, agarose is used as a thickener to give the gel an interwoven porous structure, which is used to lock in the active particles for a long time and has a good sustained-release effect. The active particles include active chlorine, active oxygen and active nitrogen, etc.

[0029] 3. The gel component in this invention is non-toxic and non-irritating. When applied to the skin, it can effectively improve skin texture and alleviate skin diseases. It avoids the side effects of oral or topical antibiotics and hormones. Furthermore, it has good biocompatibility and can be used repeatedly for a long time without causing dependence or drug resistance in the human body.

[0030] 4. The plasma-activated silver nanoflower hydrogel of this invention, combined with physiological saline, can be used by moistening the target skin area with 0.8-1.2 wt% physiological saline for 1-5 minutes before use. This can gently soften the stratum corneum of the skin, reduce skin barrier resistance, and promote the absorption of silver ions (Ag) in the hydrogel. + ) and hypochlorite (ClO) generated after plasma activation - It penetrates deeply into the skin and forms a micro-hydration layer on the moistened skin surface, making the hydrogel patch adhere more tightly, delaying hydrogel dehydration, maintaining its swollen state, avoiding the loss of active particles due to air gaps, and prolonging Ag... + With ClO - The sustained-release cycle enables long-term sustained release of active substances (lasting for more than 12 hours), synergistically enhancing the effects of silver ions and ClO. - Dual antibacterial mechanism (ClO) - Exhibits strong oxidizing properties, providing instant sterilization; Ag +It provides long-lasting antibacterial effects, covering a wider spectrum of pathogens, relieves redness, swelling, and thickened scaling, accelerates inflammation resolution, regulates immune responses, and alleviates symptoms of chronic inflammatory diseases such as psoriasis.

[0031] 5. The plasma-activated silver nanoflower hydrogel patch of the present invention, combined with physiological saline, effectively locks in the active substances generated by plasma action after plasma treatment. It also contains silver nanoparticles, which can work together with other active substances under certain conditions to produce a synergistic effect. When treating skin samples of chronic immune diseases such as psoriasis, it can reduce the levels of pro-inflammatory cytokines such as IL-17, IL-23, TNF-α, and IL-22 in the body, directly targeting the core of the Th17 immune pathway, and has anti-inflammatory and immunomodulatory effects, achieving a treatment upgrade from symptom relief to immune reconstruction. Attached Figure Description

[0032] Figure 1 This is a schematic diagram illustrating the preparation and application of the gel patch of the present invention; Figure 2 This is the scanning electron microscope structure of the plasma-activated gel patch in Embodiment 1 of the present invention; Figure 3 The scanning electron microscope structure of the silver nanoflower particles in Example 1 of this invention; Figure 4 This is a schematic diagram of the process of moistening the target skin area with physiological saline in Embodiment 1 of the present invention; Figure 5 This is an image showing the effect of treating mice with plasma-activated nano-silver gel patches combined with physiological saline in Example 1 of the present invention; Figure 6 To Figure 5 Psoriasis-like lesion area and severity score (PASI score) were calculated for mice in each treatment group. Figure 7 This image shows the serum levels of IL-17, IL-22, IL-23, and TNF-α pro-inflammatory factors in mice during the application of plasma-activated nanosilver gel patches combined with physiological saline in psoriasis, as described in Example 2 of this invention. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] Unless otherwise specified, all test materials and reagents used in the following examples are commercially available.

[0035] Unless otherwise specified in the embodiments, the techniques or conditions described in the literature in this field or in accordance with the product manual may be followed.

[0036] Example 1 The preparation and application process of a plasma-activated silver nanogel patch combined with physiological saline is as follows: Figure 1 As shown, the preparation of the gel patch includes the following steps: At room temperature, 200 μL of 0.1 M silver nitrate aqueous solution and 1 mL of 1 M polyvinylpyrrolidone aqueous solution were added to 10 mL of deionized water and magnetically stirred until the mixture was fully homogeneous. Then, 200 μL of 1 M ascorbic acid aqueous solution was injected into the stirred mixture and reacted for 10 min to prepare colloidal silver nanoflower particles with a size of approximately 500 nm. The SEM image is shown below. Figure 3 As shown in the electron microscope image, the flower-like structure of silver nanoflowers can be seen. Silver nanoflowers have multiple significant advantages, mainly manifested in antibacterial, anti-inflammatory, and immunomodulatory effects. 1 mL of anhydrous ethanol solution containing 2.1574 g of silver nanoflower particles was added to 50 mL of a 1% (w / w) agarose aqueous solution as a thickener. The mixture was heated at 90 °C for 10 min and then dropped into a 3D-printed hydrogel preparation mold. The mixture was allowed to stand until it became gel-like, and then immersed in 0.9% (w / w) physiological saline.

[0037] Before activation using plasma, the target skin area on the back of psoriatic mice was moistened with 0.9% saline solution for 5 minutes to gently soften the surface keratin. Figure 4 As shown, the gel was placed in a plasma generator in a sealed environment. The gel was activated by plasma at a distance of approximately 1 cm from the plasma source at 42 kV and 2 watts for 2 minutes. It was then applied to the target skin area. The generated active particles were injected into the gel through the plasma generator, and the active particles were held in place by a gel patch, resulting in good sustained-release and enrichment effects. The active particles included NO. 3- NO 2- H2O2, O 2- This design avoids direct or close-range contact between the plasma generator and the skin, preventing burns and ensuring safety. Furthermore, it works synergistically with the nano-silver in the hydrogel, producing a enhanced effect. It also incorporates novel active particles, ClO₂, into the hydrogel. - Reduces skin barrier resistance and promotes the growth of silver ions (Ag) in the hydrogel. +) and hypochlorite (ClO) generated after plasma activation - It penetrates deeply into the skin and forms a micro-hydration layer on the moistened skin surface, making the hydrogel patch adhere more tightly, delaying hydrogel dehydration, maintaining its swollen state, avoiding the loss of active particles due to air gaps, and prolonging Ag... + With ClO - The sustained-release period, synergistic effect of silver ions and ClO - Dual antibacterial mechanism. The treatment of psoriatic mice with plasma-activated nano-silver gel patches using physiological saline showed significant effects, effectively improving redness, swelling, and thickened scales, accelerating inflammation resolution, regulating immune responses, and alleviating symptoms of chronic inflammatory diseases such as psoriasis.

[0038] Figure 2 The scanning electron microscope (SEM) structure of the plasma-activated nanosilver gel patch in Example 1 of this invention shows that the three-dimensional network of interwoven pores in the hydrogel can effectively lock in viable substances and solve the problem of easy deactivation in liquid form.

[0039] Example 2 Preparation of a plasma-activated nano-silver gel patch combined with physiological saline and its application in psoriasis. The preparation method includes the following steps: 200 μL of 0.1 M silver nitrate aqueous solution and 1.5 mL of 1 M polyvinylpyrrolidone aqueous solution were added to 10 mL of deionized water and magnetically stirred at room temperature until the mixture was fully homogeneous. Then, 200 μL of 1 M ascorbic acid aqueous solution was injected into the stirred mixture and reacted for 15 min to prepare colloidal silver nanoflowers with a size of approximately 500 nm. 1 mL of anhydrous ethanol solution of silver nanoflower particles (containing 2.1574 g of silver nanoflower particles) was added to 50 mL of 1% agarose aqueous solution (thickener). The mixture was heated at 90 °C for 10 min and then dropped into a 3D printed hydrogel preparation mold. The mixture was allowed to stand until it became gel-like to obtain a gel, which was then immersed in 0.9% physiological saline.

[0040] The prepared nano-silver gel was removed from the soaking saline solution and then activated by plasma at a distance of about 1.5 cm from the gel at 50 kV and 5 watts for 3 minutes. The gel was then applied to the target skin area on the back of mice with psoriasis for 3 minutes. Before application, the target skin was moistened with 0.9% saline solution to gently soften the stratum corneum. The gel patch effectively locked in the active substances generated by the plasma treatment and worked together with the nano-silver to produce a synergistic effect on the skin.

[0041] Figure 5The diagram illustrates the treatment of mice with plasma-activated nano-silver gel patches combined with physiological saline. Mice were divided into 5 groups, with the following specific treatments: Control group: Healthy mice, with Vaseline applied to the target skin area on their backs; IMQ group (model group): Psoriasis mice, without other treatment; Plasma group (plasma control group): Healthy mice were treated with plasma at a distance of approximately 1.5 cm, at 50 kV and 5 watts for 3 min; IMQ+Plasma group (plasma treatment group): Psoriasis mice were treated with plasma at a distance of approximately 1.5 cm, at 50 kV and 5 watts for 3 min; Plasma-activated hydrogel group (plasma-activated hydrogel group): Mice were treated according to the method described in Example 2 of this invention. Statistical experimental results are as follows: Figure 5 As shown, the results indicated that the psoriasis model group (IMQ) mice gradually developed typical psoriasis symptoms such as erythema, thickened scales, and raised lesions during the modeling process, indicating successful model establishment. After plasma treatment (IMQ+Plasma group) and plasma-activated hydrogel patch treatment (Plasma-activated hydrogel group), the symptoms of the mice began to improve from day 5, with a decrease in the area and severity of lesions, significant reduction in erythema, thickening, and scaling, and a decrease in PASI scores, indicating that both treatments were effective. The improvement effect of the plasma-activated hydrogel patch group was particularly outstanding, superior to the plasma treatment group alone, suggesting that this patch has significant application potential in psoriasis and other chronic immune diseases.

[0042] Figure 6 To Figure 5 Mice in each treatment group were assessed using the Psoriasis Area and Severity Index (PASI) scale. The PASI scale scores mice based on a combination of the severity of skin lesions (including erythema, infiltration, and scaling) and the area of ​​the lesions. The final score is calculated using a specific formula and is commonly used to assess the severity of psoriasis vulgaris. It is an internationally recognized scoring standard for psoriasis lesion severity. The PASI scores of each mouse group demonstrate the significant therapeutic effect of the plasma-activated nano-silver gel patch combined with physiological saline in treating psoriasis.

[0043] Psoriasis is an immune-mediated inflammatory disease. The IL-23 / Th17 pathway is a key axis in the pathogenesis of psoriasis, and IL-17 is the main effector of this pathway. Overexpression of IL-17 leads to excessive epidermal proliferation and a strong inflammatory response. TNF-α is also an important cellular inflammatory factor. The levels of various cellular inflammatory factors in the serum of mice in each group were detected by ELISA. The levels of pro-inflammatory cytokines such as IL-17, IL-23, TNF-α, and IL-22 in the serum of the samples were measured using a double-antibody sandwich assay. Microplates were coated with purified mouse IL-17, IL-23, TNF-α, and IL-22 antibodies to prepare solid-phase antibodies. IL-17, IL-23, TNF-α, and IL-22 were added sequentially to the wells coated with monoclonal antibodies, followed by binding with HRP-labeled IL-17, IL-23, TNF-α, and IL-22 antibodies to form antibody-antigen-enzyme-labeled antibody complexes. After thorough washing, the substrate TMB was added for color development. TMB is converted to blue under the catalysis of HRP enzyme, and then to yellow under acidic conditions. The intensity of the color is positively correlated with the levels of IL-17, IL-23, TNF-α, and IL-22 in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the concentrations of IL-17, IL-23, TNF-α, and IL-22, cytotoxic factors, in the serum of mice in each group were calculated. These abnormal inflammatory factors may enter the spleen through the circulatory system, causing splenic inflammation and splenomegaly.

[0044] The mice were divided into 5 groups, and Figure 5 If the groups are the same, the specific processing is as follows: Control group: Healthy mice, with petroleum jelly applied to the target skin area on their backs; IMQ group: Psoriasis mice, without any other treatment; Plasma group: Healthy mice were treated with plasma at a distance of about 1.5 cm from the mice, at 50 kV and 5 watts for 3 min. IMQ+Plasma group: Psoriasis mice were treated with plasma at a distance of about 1.5 cm from the mice, at 50 kV and 5 watts for 3 min. Plasma-activated hydrogel group: mice were treated according to the method described in Example 2.

[0045] Statistical experimental results as follows Figure 7As shown in the figure, Control represents the control group (healthy mice); IMQ represents the model group (psoriasis mice); Plasma represents healthy mice treated with plasma; IMQ+Plasma represents the psoriasis mouse group treated with plasma; and Plasma-activated hydrogel represents the psoriasis mouse group treated with plasma-activated nano-silver gel patches combined with physiological saline for 3 min in Example 2. The figure shows that, compared with the control group, the serum levels of cytoinflammatory factors in the model group of psoriasis mice were increased. After treatment with plasma-activated nano-silver gel patches combined with physiological saline, the levels of pro-inflammatory cytokines IL-23, TNF-α, IL-17, and IL-22 were significantly reduced, regulating the immune response, alleviating symptoms of chronic inflammatory diseases (such as psoriasis), and enhancing the medical application potential of plasma.

[0046] This invention prepares a gel patch containing silver nanoparticles. After preparation, the gel patch is immersed in physiological saline and then treated with plasma to obtain a gel containing silver nanoparticles. Furthermore, the patch is applied to skin sample treatment and it is found to have a good inhibitory effect on skin inflammation.

[0047] To reduce the harm caused by direct plasma action on the skin surface and avoid the disadvantages of weak penetration, rapid attenuation, and insignificant effects of active particles, this invention prepares a gel patch containing silver nanoparticles. After plasma treatment, the patch can be applied to the inflamed skin surface. We also designed a method to soak the gel patch in physiological saline. This plasma activation treatment not only mitigates the damage caused by direct plasma treatment but also provides a long-term retention effect on the active particles generated by the plasma. Furthermore, the hypochlorous acid produced by the synergistic action of silver nanoparticles and plasma-activated physiological saline has excellent anti-inflammatory and antibacterial effects on the skin.

[0048] The method of this invention organically combines plasma activation technology with hydrogels and nanomaterials, which differs from previously reported methods and has better processing effects and a wider range of applications. This invention uses solid gel patches to lock ClO. - / Ag + It incorporates Ag + (Nano silver) and ClO -The invention employs a dual mechanism (hypochlorite) combined with micro-hydration layer technology (forming a moisturizing layer between the patch and the skin) to prevent the active substances from oxidizing and becoming inactive upon contact with air, extending the effective period to 72 hours. A single application can provide continuous protection for up to 12 hours, significantly improving treatment efficiency and efficacy, and further expanding its application to chronic immune diseases such as psoriasis. Furthermore, this invention involves plasma activation followed by hydrogel patch treatment, allowing even short-lived active substances to exert their effects immediately. It also innovatively combines plasma activation of saline solution to generate ClO₂. - With silver nanoflower sustained-release Ag + This forms a dual-active system with a wide range of applications. Pretreatment of the skin with physiological saline softens the stratum corneum and reduces barrier resistance, significantly increasing Ag levels. + / ClO - It has a deep penetration capability; combined with the three-dimensional network structure of the gel, it achieves long-term sustained release of active substances (lasting for more than 12 hours).

[0049] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing plasma-activated silver nanogel, characterized in that: Includes the following steps: S1. Mix silver nanoflower particles with agarose solution, heat to react, and let stand until it becomes gel-like. S2. Soak the gelatinous substance obtained in S1 in physiological saline; S3. The gel-like substance treated in S2 is subjected to plasma treatment to obtain the plasma-activated nano-silver gel.

2. The method for preparing plasma-activated silver nanogel according to claim 1, characterized in that: In S1, the size of the silver nanoflower particles is 500 nm.

3. The method for preparing plasma-activated silver nanogel according to claim 1, characterized in that: In S1, the preparation method of the silver nanoflower particles includes the following steps: mixing silver nitrate aqueous solution, polyvinylpyrrolidone aqueous solution and water evenly, adding ascorbic acid aqueous solution to obtain a reaction solution, and stirring the reaction solution to obtain the silver nanoflower particles.

4. The method for preparing plasma-activated silver nanogel according to claim 1, characterized in that: In S1, the mass fraction of the agarose solution is 1%; the mass-volume ratio of silver nanoflower particles to agarose solution is 2-3 g:50 mL.

5. The method for preparing plasma-activated silver nanogel according to claim 1, characterized in that: In S1, the reaction temperature is 85-95 °C and the time is 5-15 min.

6. The method for preparing plasma-activated silver nanogel according to claim 1, characterized in that: In S2, the mass concentration of the physiological saline is 0.8-1.2 wt%.

7. The method for preparing plasma-activated silver nanogel according to claim 1, characterized in that: In S3, during the plasma treatment process, the distance between the plasma source and the gel-like material to be treated is 1-1.5 cm, the voltage is 42-50 kV, the power is 2-5 watts, and the treatment time is 2-3 min.

8. A plasma-activated silver nanogel, characterized in that: It was prepared by the method for preparing plasma-activated silver nanogel as described in any one of claims 1-7.

9. The use of the plasma-activated nanosilver gel as described in claim 8 in the preparation of a patch for treating psoriasis.

10. A gel patch, characterized in that: It contains the plasma-activated silver nanogel as described in claim 8.

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