A cell crawling sheet test device facilitating the taking out of a glass sheet

Abstract

The application discloses a cell climbing slide test device facilitating taking out slides, which comprises a multi-well plate, a well plate cover and slides, a plurality of slide pressing and magnetic suction taking-out mechanisms are fixed on the well plate cover and correspond to positions of liquid adding holes, the slide pressing and magnetic suction taking-out mechanisms can be inserted into the liquid adding holes, the slide pressing and magnetic suction taking-out mechanisms comprise connecting observation barrels, a ferrite magnet ring is arranged at the bottom of each connecting observation barrel, the ferrite magnet ring is fixed on the connecting observation barrel through a connecting rod, and a ferrite magnetic suction ring is fixedly attached to the edge of each slide. The slide pressing and magnetic suction taking-out mechanisms of the device can press and tightly adhere the slides to the bottom of the holes, so that the problem of slide floating in the cell culture process is avoided; when the well plate cover is opened after the cell culture is completed, the slide pressing and magnetic suction taking-out mechanisms can directly suction the slides from the bottom of the holes and take out the slides from the liquid adding holes, so that the taking-out is extremely convenient.

Description

Technical Field

[0001] This invention relates to the field of cell biology technology, and more specifically to a cell slide assay device that facilitates the removal of glass slides. Background Technology

[0002] Cell slide culture is a widely used experimental method in cell biology research. Its core principle is to culture cells on a specially designed glass or plastic surface, allowing the cells to adhere well and spread naturally. By completely immersing the slide in the cell culture medium, cell slide culture provides an ideal microenvironment for cell growth, offering significant advantages over traditional cell culture methods.

[0003] However, current cell slide technology has the following drawbacks: 1. The slides tend to float in liquid culture medium, resulting in a double-layer cell adhesion; 2. The slides are tightly attached to the bottom of the wells of the plate, and the area of ​​the slides is slightly smaller than the area of ​​the bottom of the wells, making it extremely inconvenient to remove the slides from the bottom of the wells; 3. The area of ​​the bottom of the wells of the plate is larger than the area of ​​the slides. When too much cell suspension is added, the cells may cover the entire bottom of the wells, which may lead to cell edge aggregation, that is, the cell density is high at the edges and the number of cells in the center is low. Summary of the Invention

[0004] To address this, the present invention provides a cell slide assay device that facilitates the removal of glass slides. This device features a magnetic slide-pressing and extraction mechanism on the well plate cover, corresponding to the liquid filling holes. This mechanism presses the glass slide firmly against the bottom of the well, preventing it from floating during cell culture. Furthermore, after cell culture is complete, when the well plate cover is opened, the magnetic slide-pressing and extraction mechanism can directly lift the glass slide from the bottom of the well and remove it from the liquid filling hole, making removal extremely convenient. The glass slide of this device has a microgroove in the center, with several micropillars within the microgroove, facilitating cell adsorption and growth in the center of the slide while inhibiting cell edge aggregation.

[0005] To achieve the above-mentioned technical effects, the present invention is implemented through the following technical solution: A cell slide assay apparatus for easy slide removal includes a multi-well plate, a well plate cover, and glass slides. The well plate cover is fastened to the top of the multi-well plate. The multi-well plate has several liquid addition holes. Several magnetic slide extraction mechanisms are fixed to the well plate cover, with each mechanism corresponding to one of the liquid addition holes and capable of being inserted into one of them. Each magnetic slide extraction mechanism includes a connecting observation tube, the bottom of which is equipped with an iron... Ferrite magnetic rings are fixed to the observation tube via connecting rods. Ferrite magnetic rings are attached to the edge of the glass slide. The glass slide is placed at the bottom of the liquid filling hole. When the orifice plate cover is fastened to the multi-hole plate, the observation tube is inserted into the liquid filling hole, and the ferrite magnetic rings press the glass slide tightly against the bottom of the liquid filling hole. When the orifice plate cover is removed from the multi-hole plate, the glass slide will be attracted to the ferrite magnetic rings along with them and removed from the liquid filling hole.

[0006] Furthermore, a microgroove is formed in the center of the glass slide, and several micropillars are disposed in the microgroove.

[0007] The beneficial effects of this invention are as follows: The device is equipped with a slide clamping and magnetic extraction mechanism on the well plate cover, corresponding to the liquid addition hole. This mechanism can clamp the slide tightly against the bottom of the well, preventing the slide from floating up during cell culture. Furthermore, after cell culture is completed, when the well plate cover is opened, the slide clamping and magnetic extraction mechanism can directly lift the slide from the bottom of the well and remove it from the liquid addition hole, making removal extremely convenient. The slide of this device has a microgroove in the center, and several micropillars are arranged in the microgroove, which facilitates cell adsorption and growth in the center of the slide, while inhibiting cell edge aggregation. The connected observation tube allows researchers to observe the cell growth morphology, and the window structure of the connecting rod facilitates the free flow of the culture medium. Attached Figure Description

[0008] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0009] Figure 1 This is a schematic diagram of the disassembled structure of a cell slide assay apparatus that facilitates the removal of glass slides; Figure 2 This is a schematic cross-sectional view of the perforated plate and the perforated plate cover when they are fastened together. Figure 3 This is a schematic diagram of the structure of the glass slide.

[0010] The attached diagram lists the components represented by each number as follows: 1-Perforated plate, 2-Perforated plate cover, 3-Connecting observation tube, 4-Ferrite magnet ring, 5-Connecting rod, 6-Slide, 7-Ferrite magnetic ring, 61-Microgroove, 62-Micro column. Detailed Implementation

[0011] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0012] like Figures 1-2 As shown, a cell slide assay device for easy slide removal includes a multi-well plate 1, a well plate cover 2, and glass slides 6. The well plate cover 2 is fastened to the top of the multi-well plate 1. The multi-well plate has several liquid inlets. Several glass slide clamping magnetic extraction mechanisms are fixed on the well plate cover, and the positions of the glass slide clamping magnetic extraction mechanisms correspond to the liquid inlets. The glass slide clamping magnetic extraction mechanisms can be inserted into the liquid inlets. Each glass slide clamping magnetic extraction mechanism includes a connecting observation tube 3, and the bottom of the connecting observation tube is provided with... A ferrite magnet ring 4 is provided, which is fixed to the connecting observation tube by a connecting rod 5. A ferrite magnetic ring 7 is attached to the edge of the glass slide. The glass slide is placed at the bottom of the liquid filling hole. When the orifice plate cover is fastened to the multi-hole plate, the connecting observation tube is inserted into the liquid filling hole and the ferrite magnet ring presses the glass slide tightly against the bottom of the liquid filling hole. When the orifice plate cover is removed from the multi-hole plate, the glass slide will be attracted to the ferrite magnet ring along with it and removed from the liquid filling hole.

[0013] like Figure 3 As shown, a microgroove 61 is formed in the center of the glass slide 6, and a plurality of micropillars 62 are disposed in the microgroove.

[0014] One specific application of this device is as follows: First, add a small amount of DPBS (20 μL, used to adhere the slides) to the bottom of the wells of the multi-well plate. Use tweezers to hold the slide and place it in the center of the well bottom, ensuring there are no gaps or air bubbles between the slide and the bottom of the well. Then, digest the cells with trypsin, count and resuspend the cells in complete culture medium. Select an appropriate cell density and seed them into the microgroove in the center of the slide. Then, close the well plate cover. At this time, insert the observation tube into the well. The ferrite magnetic ring at the bottom of the observation tube will press the slide tightly against the bottom of the well. Place the multi-well plate in an incubator for culture. During the culture, cell morphology can be observed through the hollow cavity of the connecting tube. After the culture is completed, open the well plate cover 2. At this time, the ferrite magnetic ring will be removed from the well along with the observation tube. The ferrite magnetic ring is pulled up from the bottom of the well by the magnetic attraction of the ferrite magnetic ring and is taken out of the well along with the slide. This removal method is extremely convenient and will not contaminate the cultured cells.

[0015] The ferrite magnet ring and ferrite magnetic ring in this embodiment have good resistance to acids, alkalis and organic solvents, and can be well applied in cell climbing experiments.

[0016] In the description of this specification, references to terms such as "an embodiment," "example," and "specific example" indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

Claims

1. A cell slide assay apparatus for easy removal of glass slides, comprising a multi-well plate, a well plate cover, and glass slides, wherein the well plate cover is fastened to the top of the multi-well plate, and the multi-well plate has a plurality of liquid addition holes, characterized in that, Several slide-pressing magnetic extraction mechanisms are fixed on the orifice plate cover. These mechanisms correspond to the positions of the liquid filling holes and can be inserted into them. Each mechanism includes a connecting observation tube with a ferrite magnet ring at its bottom. The ferrite magnet ring is fixed to the observation tube via a connecting rod. A ferrite magnetic ring is attached to the edge of the slide. The slide is placed at the bottom of the liquid filling hole. When the orifice plate cover is fastened to the multi-hole plate, the connecting observation tube is inserted into the liquid filling hole, and the ferrite magnet ring presses the slide firmly against the bottom of the hole. When the orifice plate cover is removed from the multi-hole plate, the slide, along with the ferrite magnet ring, is attracted to the ferrite magnet ring and removed from the liquid filling hole.

2. The cell slide assay apparatus for easy removal of glass slides according to claim 1, characterized in that, The glass slide has a microgroove in the center, and several micropillars are arranged in the microgroove.

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