Broiler feed based on complex of anti-inflammatory plant extracts and preparation method thereof

Abstract

The application discloses a kind of meat chicken feed based on anti-inflammatory plant extract compound and preparation method thereof, belong to feed processing technology field.The method will eucommia leaf powder and coarse coffee residue are mixed according to proportion to constitute fermentation substrate, prevent paste and harden using coffee residue to construct porous framework;Inoculate substrate induction and surface active pretreatment bacillus subtilis, carry out aerobic fermentation under the control of temperature and humidity linkage feedback, promote a large number of free release of bound state chlorogenic acid, and realize in situ biochemical detoxification of free caffeine.Fermentation mature material is dried and crushed to obtain fermentation raw powder, then is compounded with microencapsulated sodium butyrate of core-shell double-layer structure, and is mixed into daily ration granulation by adopting multi-stage premixing, grease liquid bridge adhesion and steam hydrothermal trigger in-situ crosslinking coating process.The application solves the problem that eucommia fermentation system is easy to collapse, realizes efficient extraction of active ingredients, preparation stress protection and accurate intestinal targeted delivery in later stage, and can significantly improve the intestinal health and production performance of meat chicken.

Description

Technical Field

[0001] This invention belongs to the field of feed processing technology, specifically to a broiler feed based on anti-inflammatory plant extracts and its preparation method. Background Technology

[0002] In modern high-density intensive broiler farming, broilers are highly susceptible to intestinal mucosal damage and necrotizing enteritis due to the combined effects of environmental stress, anti-nutritional factors in feed, and pathogenic microorganisms. With the implementation of the global feed industry's "comprehensive ban on antibiotics (prohibition of the addition of growth-promoting antibiotics)," the use of natural plant extracts (such as plant-derived polyphenols and chlorogenic acid) combined with microecological preparations to replace antibiotics for intestinal health intervention has become the mainstream research and development direction in the current feed additive field.

[0003] Eucommia ulmoides leaves are rich in eucommia polyphenols (mainly chlorogenic acid) and eucommia polysaccharides, which have good antibacterial and anti-inflammatory effects. However, the chlorogenic acid in Eucommia ulmoides leaves exists mostly in a "bound" form, encapsulated by tough plant cell walls and layers of hydrophobic "eucommia gum". Broiler chickens have relatively short digestive tracts, and their food emptying time is usually only 3-4 hours. The conventional gastrointestinal fluids and digestive enzymes of poultry are insufficient to effectively degrade the eucommia gum and cell wall barrier in a short time. If untreated Eucommia ulmoides leaves are crushed and directly mixed into the diet, the bound chlorogenic acid is difficult to release effectively and be absorbed by the intestines, resulting in a low actual bioavailability.

[0004] To improve the degree of chlorogenic acid release and utilization rate, existing technologies mainly employ two types of pretreatment processes, but both have certain limitations: Liquid phase solvent extraction: Traditional processes often use high-temperature boiling or organic solvent alcohol precipitation to purify chlorogenic acid. This type of process is not only energy-intensive, but also produces a large amount of waste residue rich in crude fiber and dietary polysaccharides after extraction, making it difficult to achieve 100% feed utilization of the entire material. At the same time, it is impossible to retain a live bacteria system that is beneficial to the intestinal microecology in the final product.

[0005] Conventional solid-state aerobic fermentation utilizes microbial fermentation to produce enzymes and break down cell walls, a cost-effective approach that balances component release with the preservation of probiotics. However, the eucommia gum and polysaccharides abundant in Eucommia leaves readily absorb water and swell, forming high-viscosity flocs. In actual production, this leads to severe gelatinization and compaction of the entire fermentation bed, significantly reducing material porosity. For aerobic bacteria such as Bacillus subtilis, which decompose cell walls, decreased permeability can cause severe oxygen deficiency in the fermentation system, resulting in slow fermentation start-up, proliferation of unwanted microorganisms, and even system collapse. Summary of the Invention

[0006] To overcome the shortcomings of existing technologies, this invention provides a broiler feed based on anti-inflammatory plant extracts and its preparation method. This invention primarily addresses the problem that Eucommia ulmoides polysaccharides and gums gelatinize and clump upon contact with water, making it difficult for existing extraction processes to efficiently extract free chlorogenic acid from Eucommia ulmoides leaves.

[0007] According to one aspect of the present invention, a method for preparing broiler feed based on anti-inflammatory plant extracts is provided, comprising the following steps: S1: Pulverize dried Eucommia ulmoides leaves to obtain Eucommia ulmoides leaf powder; pulverize coffee grounds and pass them through a 20-40 mesh sieve to obtain coarse coffee grounds; then mix Eucommia ulmoides leaf powder and coarse coffee grounds in a weight ratio of 6:4-8:2 to form a mixed fermentation substrate; S2: Add pretreated Bacillus subtilis solution to the mixed fermentation substrate, and add water to adjust the water content of the system to 45% to 55% to obtain fermented wet material; S3. Place the wet fermentation material in an aerobic environment for solid-state aerobic fermentation, control the fermentation temperature at 32℃~37℃, and the fermentation cycle at 48~72 hours to obtain fermented cooked material; S4: The fermented material is directly dried at low temperature, with the drying temperature controlled at 50℃~60℃. After the moisture content is less than 10%, it is homogenized and pulverized to obtain bio-fermented raw powder rich in free chlorogenic acid. S5: The bio-fermented raw powder and microencapsulated sodium butyrate are mixed evenly at a weight ratio of 90:10 to 95:5 to obtain a compound anti-inflammatory feed additive. Then, it is added to the broiler basal diet (a conventional bulk powdered feed used to meet the basic energy and protein requirements of broilers for daily growth, such as corn-soybean meal basal diet or wheat-soybean meal basal diet) for mixing and pelleting.

[0008] In existing technologies, when chlorogenic acid is extracted from Eucommia ulmoides leaves through microbial fermentation, the leaves are rich in Eucommia ulmoides polysaccharides and gums, which easily absorb water and swell to form dense, high-viscosity micelles. This leads to material gelatinization, oxygen deficiency and collapse of the aerobic fermentation system, and traditional liquid-phase extraction has problems such as complex processes, loss of live bacteria and polysaccharides, and waste residue pollution. Furthermore, using coffee grounds directly as feed can lead to caffeine toxicity.

[0009] Working Principle: The incompressible, rigid lignocellulose structure of coarse-grained coffee grounds serves as a porous physical framework, interspersed among ultrafine Eucommia ulmoides leaf powder. This forcefully expands the viscous matrix, maintaining the high porosity of the fermentation bed. Under sufficient oxygen, Bacillus subtilis proliferates and secretes enzymes, disrupting the cell walls of Eucommia ulmoides leaves along the pores to release chlorogenic acid. Simultaneously, the fermentation system achieves in-situ biochemical detoxification of the coffee grounds. During fermentation in step S3, Bacillus subtilis produces enzymes that break down cell walls, prompting the release of large amounts of free Eucommia ulmoides polyphenols and tannins. The released polyphenols spontaneously generate hydrogen bonds and hydrophobic complexes with the free caffeine on the surface of the coffee grounds in a warm and humid environment, forming in-situ large-molecule polyphenol-caffeine insoluble complexes that cannot be dissociated and absorbed in the short digestive tract of poultry. These complexes are safely excreted with feces, thus eliminating the alkaloid neurotoxic side effects of direct feeding of coffee grounds and successfully overcoming the technical toxicity defect that prevents the direct large-dose feeding of single coffee grounds. After fermentation, the microorganisms are converted into dormant Bacillus subtilis spores by direct drying at low temperature, and finally microencapsulated sodium butyrate is released at specific points.

[0010] This research breaks through the technical bias of easy gelatinization during the fermentation of Eucommia ulmoides leaves, significantly improving the conversion rate of bound chlorogenic acid to free chlorogenic acid; it also desensitizes the toxic side effects of caffeine in situ through biochemical coupling reactions, enabling direct drying and 100% feed utilization of the entire fermented material without solvent extraction; and finally constructs a three-dimensional synergistic anti-inflammatory network system that utilizes free chlorogenic acid for sterilization and anti-inflammation, live bacterial spores for oxygen consumption and antibacterial activity, Eucommia ulmoides polysaccharides as prebiotics to promote growth, and butyrate to target and repair the intestinal mucosa.

[0011] Preferably, in step S2, the pretreatment of Bacillus subtilis liquid includes: first, placing it in a liquid culture medium containing 0.5% to 1.0% Eucommia ulmoides leaf decoction for 6 to 8 hours until the logarithmic growth phase; then, mixing in tea saponin at a mass concentration of 0.1% to 0.2% into the Bacillus subtilis liquid. The pretreated Bacillus subtilis solution was inoculated into the mixed fermentation substrate using an aerosol micro-mist spray method, with an initial viable count of 1.0 × 10⁻⁶ per gram of wet fermentation material. 8 ~5.0×10 8 CFU.

[0012] Eucommia leaves are rich in natural antibacterial substances such as tannins and extremely hydrophobic eucommia gum. Direct inoculation with conventional commercial dormant bacterial strains results in an extremely long growth retardation period, and water and bacteria have difficulty penetrating the surface hydrophobic gelatinous layer, leading to slow fermentation initiation and easy occupation of ecological niches by environmental bacteria.

[0013] In this scheme, targeting the hydrophobic eucommia gum and natural antibacterial components on the surface of Eucommia ulmoides leaves, the strain was first pre-cultured with substrate induction in a culture medium containing a decoction of Eucommia ulmoides leaves. Pre-culture with an extract containing the target substrate prompted the strain to express specific enzymes that degrade specific cell walls in advance, thus accelerating the fermentation start-up process after inoculation.

[0014] During inoculation, the natural nonionic surfactant tea saponin is mixed in to reduce the surface tension of the bacterial solution. Combined with aerosol micro-atomization spraying, the capillary action of the coarse coffee grounds allows micron-sized droplets to penetrate into the hydrophobic interface. The combination of the surfactant and the physical action of aerosol micro-atomization enables the bacterial solution to effectively overcome the hydrophobic repulsion of eucommia gum. This promotes early and uniform colonization of highly active strains in the deep pores of the substrate, providing a good foundation for subsequent stable enzyme production and chlorogenic acid release.

[0015] The water-boiled extract of Eucommia ulmoides leaves can be obtained through a conventional process of boiling with water and removing residue.

[0016] Preferably, in step S3, the solid-state aerobic fermentation adopts a staged temperature and humidity feedback control method: the relative humidity of the environment is maintained at 85% to 95% within 24 hours before fermentation; when the temperature of the center of the fermentation bed rises to 36°C, pulsed cross-flow ventilation and cold mist humidification are activated to allow the airflow to diffuse along the internal pore channels formed by coarse-grained coffee grounds, so as to control the temperature difference between the center and the surface of the fermentation bed to not exceed 3°C, and maintain the fermentation bed in the enzymatic hydrolysis temperature range of 32°C to 37°C; at the same time, the relative humidity of the fermentation bed environment is maintained in the range of 75% to 85%.

[0017] If a constant high humidity of 85%–95% is maintained during fermentation, it will lead to an explosive proliferation of microorganisms in the middle and late stages of fermentation, generating intense "biological heat" (locally exceeding 50°C). Under high humidity, the heat is extremely difficult to dissipate, which can easily lead to excessively high internal temperatures in the material pile, causing the live bacteria to become inactivated and the extracted free chlorogenic acid to undergo high-temperature oxidation and browning, thus becoming ineffective.

[0018] This solution employs a temperature and humidity linkage feedback control mechanism during the solid-state fermentation stage. A bed center temperature of 36℃ is used as the trigger threshold to activate pulsed cross-flow ventilation and cold mist humidification. The ventilation airflow diffuses through the internal pores of the coffee grounds, carrying away heat and exhaust gases. The cold mist further cools the coffee grounds by absorbing heat through moisture vaporization, maintaining the temperature difference between the center and surface within 3℃ and upholding the set temperature and relative humidity ranges.

[0019] This core temperature-triggered pulsed ventilation and cold mist humidification linkage intervention establishes a dynamic balance between "water retention" and "heat dissipation" within the fermentation bed. It alleviates the problem of live bacteria inactivation or free chlorogenic acid oxidation and browning caused by excessive local bioheat accumulation in the middle and late stages of fermentation, thereby improving the stability of fermentation batch quality during industrial-scale production.

[0020] Preferably, in step S5, the microencapsulated sodium butyrate has a core-shell bilayer structure, with an outer wall material of resistant starch and an inner wall material of a lipid layer of hydrogenated palm oil and sunflower lecithin melted together at a mass ratio of 4:1 to 6:1 with a melting point of 50°C to 60°C. The leakage rate of this microencapsulated sodium butyrate is less than 10% after 2 hours in simulated avian gizzard fluid with pH ≤ 3.0 and mechanical grinding, while the release rate is greater than 90% in simulated posterior intestinal fluid rich in specific bile salts and pancreatic lipase with a pH of 6.5 to 7.5.

[0021] The technical problem to be solved: Broilers have extremely high body temperatures (41-42℃) and possess a proventriculus that secretes strong acids and a gizzard that undergoes intense physical grinding. If only fatty acids or vegetable oils are used for coating, the sodium butyrate is easily ground up and melted in the gizzard, causing premature leakage of sodium butyrate in the stomach, irritating the gastric mucosa, and completely failing to reach the middle and lower intestines where enteritis is prone to occur.

[0022] Working Principle: A double-locking wall material specifically designed for broiler physiological structure defines a dual-layer stress-resistant structure for microencapsulated sodium butyrate. The outer shell is a resistant starch layer, and the inner layer is a mixture of hydrogenated palm oil and sunflower lecithin lipids with a melting point of 50℃~60℃. In a simulated forestomach segment with pH ≤ 3.0 and accompanied by mechanical grinding, the leakage rate is less than 10%. In simulated mid-to-posterior intestinal fluid rich in specific bile salts and pancreatic lipases, the lipids dissociate, releasing butyrate and lecithin at a release rate greater than 90%. The lipid shell of this composite microcapsule only undergoes specific targeted enzymatic breakdown when it enters the ileocecal segment filled with specific pancreatic lipases and bile salts.

[0023] The resistant starch shell provides mechanical rigidity to cushion against the intense physical abrasion of the avian gizzard; the high-melting-point lipid layer provides a barrier against the strong acidity of the proventriculus. The bilayer structure effectively reduces premature leakage of sodium butyrate in the forestomach, minimizing physical irritation to the gastric mucosa and masking the butyrate odor; after entering the ileocecal segment, which is prone to enteritis, the composite microcapsules are degraded by specific enzymes, achieving targeted release of butyrate ions; simultaneously, the dissociated lecithin can serve as a lipid material, synergistically participating with butyrate ions in the repair of damaged intestinal mucosal cell membranes.

[0024] Preferably, the compound anti-inflammatory feed additive accounts for 0.1% to 0.5% of the total mass of the final broiler feed. Furthermore, when mixing the compound anti-inflammatory feed additive into the basal diet of broilers, a step-by-step premixing and liquid bridge adhesion process is adopted: first, the compound anti-inflammatory feed additive and porous aluminum silicate with a particle size of 60-80 mesh are premixed at a weight ratio of 1:5. Then, an equal-volume incremental mixing process is used to gradually mix it into the basal diet powder. Finally, 0.5% to 1.0% of vegetable oil, accounting for 0.5% to 1.0% of the total weight of the basal diet, is sprayed in as a liquid bridge binder for total mixing. The surface of the ungranulated basal diet coarse powder is mixed, and the coefficient of variation of the uniformity of the additive distribution in the final broiler feed is not greater than 5%.

[0025] Technical problem to be solved: 0.1% to 0.5% is considered a trace additive in the ton-scale giant mixers of the feed industry. Direct pouring is highly susceptible to severe stratification and segregation due to electrostatic adsorption of fine powder or differences in powder specific gravity. This leads to uneven feed intake among large flocks (some cannot eat and have no effect, while others overeat and develop osmotic diarrhea), seriously affecting the uniformity of the chickens at slaughter.

[0026] Therefore, in this invention, porous aluminosilicate salts are first used to adsorb fine powder to reduce free static electricity. Then, through an equal-volume incremental mixing process, the powder is incorporated into the ungranulated basal diet coarse powder. Finally, a small amount of vegetable oil is sprayed in as a liquid bridge, utilizing the surface tension of the oil to adhere and fix the additive powder to the surface of the original grain particles. Addressing the problem of stratification and segregation that easily occurs with extremely small amounts (0.1%–0.5%) in industrial mixers, the use of porous aluminosilicate salts to eliminate static electricity, standardized equal-volume incremental dilution, and the combination of vegetable oil liquid bridge adhesion effectively reduces the tendency for segregation caused by differences in specific gravity and particle size. The coefficient of variation for the finished product's mixing uniformity is controlled below 5%, ensuring the uniformity of the effective component dosage in the poultry flock and helping to maintain the uniformity of the flock's growth.

[0027] This has enabled the transformation from "laboratory theoretical formulation" to "large-scale industrial production." By mandating a final product variation coefficient of ≤5%, the efficient dispersion and anti-stratification of trace complexes are ensured from a physical process perspective, guaranteeing that every broiler chicken's feed contains a precisely proportioned anti-inflammatory matrix combination.

[0028] Preferably, in step S5, before adding the compound anti-inflammatory feed additive to the broiler basal diet for mixing and pelleting, 2% to 4% of sodium alginate and calcium carbonate compound micro powder by weight is premixed into the compound anti-inflammatory feed additive; in the subsequent steam conditioning process at 75°C to 85°C, the steam moisture triggers the cross-linking reaction between sodium alginate and calcium ions, forming a calcium alginate protective layer in situ on the outer surface of the compound anti-inflammatory feed additive particles, thereby reducing the thermal and mechanical damage to the live bacteria, chlorogenic acid, and microencapsulated sodium butyrate encapsulation structure in the fermented raw powder during the conditioning and pelleting process.

[0029] The technical problem to be solved: High-temperature steam conditioning at around 80°C and strong mechanical extrusion in the feed industry directly lead to the death of live bacteria, pyrolysis of chlorogenic acid, and melting and rupture of the outer shell of sodium butyrate microcapsules, thus losing the significance of targeted release into the late intestine.

[0030] The processing steps for generating a hydrogel protective layer in situ during the steam conditioning stage are specified. When sodium alginate and calcium carbonate in their dry powder state encounter high-temperature and high-humidity steam (75℃~85℃) provided by the granulation process, moisture acts as a medium to dissolve sodium alginate and promote the release of free calcium ions from calcium carbonate. The two undergo an ionic cross-linking reaction, generating a calcium alginate hydrogel film with lubricating and buffering properties in situ on the particle surface.

[0031] By utilizing the high-temperature and high-humidity steam inevitably generated during the feed conditioning process as the triggering condition for the cross-linking reaction, in-situ coating of powders can be achieved without the need for complex external liquid-phase coating equipment. The solidified network hydrogel layer has certain specific heat capacity and lubrication properties, which alleviates heat conduction and mechanical shear force during subsequent extrusion, and reduces secondary damage to heat-sensitive components (such as live bacteria, free chlorogenic acid, and sodium butyrate microcapsules).

[0032] In another aspect of the present invention, a broiler feed based on an anti-inflammatory plant extract compound is provided, the broiler feed comprising a broiler basal diet and compound anti-inflammatory granules dispersed in the basal diet; the compound anti-inflammatory granules include: The skeletal phase formed by coarse-grained coffee grounds; the Eucommia ulmoides leaf fermentation component attached to the surface and pores of the skeletal phase; Bacillus subtilis dormant spores dispersed in the fermentation phase; and microencapsulated sodium butyrate distributed on the periphery or in the pores of the composite anti-inflammatory particles; wherein the outer surface of the composite anti-inflammatory particles is coated with a calcium alginate protective layer.

[0033] Preferably, the broiler feed comprises a broiler basal diet and a compound anti-inflammatory feed additive dispersed on its surface; the compound anti-inflammatory feed additive uses coarse-grained coffee grounds with an internal porous physical framework as a carrier, and the surface and pores of the carrier are embedded with Eucommia ulmoides leaf fermentation components, free chlorogenic acid, Bacillus subtilis Bacillus subtilis dormant spores and microencapsulated sodium butyrate, and is externally coated with a calcium alginate protective layer.

[0034] Preferably, the compound anti-inflammatory feed additive is fixedly attached to the surface of the broiler basal diet pellets in the form of compound anti-inflammatory granules. The outer surface of the compound anti-inflammatory granules is coated with a calcium alginate protective layer, and the coefficient of variation of the distribution and mixing uniformity of the compound anti-inflammatory feed additive in the finished broiler feed is not greater than 5%.

[0035] Preferably, the mass concentration of free chlorogenic acid in the finished broiler feed pellets is not less than 4 mg / kg, and the number of dormant Bacillus subtilis surviving in the pellets is not less than 2.0 × 10⁻⁶. 6 CFU / g.

[0036] The beneficial effects of this invention are as follows: 1. This invention utilizes the incompressible, rigid structure of coarse-grained coffee grounds as a physical framework to effectively expand the viscous matrix formed by the swelling of Eucommia ulmoides gum upon contact with water. This maintains the excellent high porosity and permeability of the fermentation bed, promoting the efficient proliferation and enzyme production of aerobic Bacillus subtilis. This process converts a large amount of the highly difficult-to-digest bound chlorogenic acid into a free state. Simultaneously, the polyphenols released during fermentation spontaneously complex with the free caffeine on the surface of the coffee grounds to form insoluble substances, thus eliminating the neurotoxicity of the coffee grounds in situ. This achieves safe, solvent-free extraction and utilization of the entire material for animal feed.

[0037] 2. This invention addresses the extremely hydrophobic nature of Eucommia ulmoides leaves by employing substrate extract pre-culturing and tea saponin micro-atomization inoculation, overcoming the hydrophobic repulsion force at the interface and accelerating the deep penetration and colonization of live bacteria. It also pioneers a pulse-flow ventilation and cold mist humidification linkage feedback mechanism triggered by core temperature, maintaining a dynamic balance between heat dissipation and water retention in the fermentation bed, effectively preventing the large-scale inactivation of live bacteria and the thermal oxidation browning of heat-sensitive chlorogenic acid caused by local high temperatures.

[0038] 3. This invention addresses the unique physiological structure of broilers, characterized by high body temperature, strong proventricular acid, and intense physical grinding in the gizzard. It employs a double-layer microencapsulation design: a resistant starch outer shell and a high-melting-point lipid inner shell. The outer layer resists mechanical grinding, while the inner layer blocks strong acids, effectively preventing premature leakage of sodium butyrate in the forestomach. This ensures that sodium butyrate dissociates only in the ileocecal region, rich in specific enzymes, precisely releasing butyrate ions. This, in turn, synergistically forms a three-dimensional anti-inflammatory network in the intestine with free chlorogenic acid.

[0039] 4. This invention cleverly utilizes the high-temperature and high-humidity steam environment inherent in the conventional feed pelleting process to trigger in-situ cross-linking of sodium alginate and calcium ions on the material surface, generating a calcium alginate protective film. This film provides excellent buffering against heat transfer and mechanical shear forces, greatly reducing secondary damage to live bacteria and microcapsule structures. Simultaneously, by combining porous aluminosilicate to eliminate static electricity and the incremental addition of vegetable oil liquid bridging technology, the invention successfully overcomes the challenge of stratification and segregation of trace components in giant mixers, ensuring uniform and consistent dosage of medication consumed by chickens. Detailed Implementation

[0040] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments. Example 1:

[0041] The specific steps for preparing compound anti-inflammatory broiler feed are as follows: S1: Eucommia leaves with a moisture content of less than 10% are crushed into Eucommia leaf powder. At the same time, coffee grounds are crushed and passed through a 30-mesh sieve. The sieve residue is collected to obtain coarse-grained coffee grounds. 7.0 kg of the above Eucommia leaf powder and 3.0 kg of the above coarse-grained coffee grounds (weight ratio 7:3) are accurately weighed and put into a V-type dry powder mixer. The mixture is dry-mixed at room temperature for 10 minutes to obtain 10.0 kg of loose-textured mixed fermentation substrate with uniformly distributed internal woody pores. S2: Accurately weigh 100g of dried pure Eucommia ulmoides leaf powder, add it to 1000mL of purified water, boil and reflux at 100℃ for 1 hour, filter it through a 200-mesh filter cloth while hot to remove residue, and collect the filtrate as Eucommia ulmoides leaf decoction. The above-mentioned water-boiling extract was added to conventional liquid LB medium at a volume fraction of 0.8% (v / v). Commercial feed-grade Bacillus subtilis strain was then inoculated and cultured in a constant temperature shaker at 37°C and 150 rpm for 6 hours to allow it to reach the logarithmic growth phase and express the cell wall-degrading enzyme system ahead of schedule. Add 0.15% (w / w) of natural tea saponin powder to the cultured live bacteria solution and stir magnetically at a uniform speed until completely dissolved to reduce the surface tension of the solution. Spread 10.0 kg of the mixed fermentation substrate evenly in a mixing tank. Use a high-pressure ultrasonic aerosol atomization system (adjust the nozzle to ensure a median droplet size of 20-30 μm) to evenly spray the bacterial solution into the substrate, while simultaneously adding sterile pure water until the total moisture content of the substrate system reaches an accurate 50%, as measured by a rapid moisture analyzer. Plate count analysis showed an initial viable count of 3.0 × 10⁻⁶ cells per gram of wet fermentation material. 8 CFU; S3: Transfer the wet fermentation material into a solid fermentation chamber with a porous flow plate at the bottom, and strictly control the thickness of the spread material to 30cm. Insert PT100 high-precision temperature sensors at the center (15cm depth) and the surface of the fermentation bed, and connect them to the automated temperature and humidity PLC control cabinet.

[0042] Induction mass transfer period (0-24h): Close the chamber door, set the basic temperature inside the chamber to 32℃, turn on the ultrasonic humidifier to maintain the relative humidity inside the chamber at a constant 90%, and let it ferment statically. Interlocking heat dissipation period (24-60h): When the sensor detects that the temperature at the center of the fermentation bed has risen sharply to the threshold of 36℃ due to biological heat, the physical intervention program is automatically triggered: the bottom variable frequency fan is turned on for pulsed cross-flow ventilation (upward wind speed 0.5m / s, executing a cycle of "blowing for 5 minutes - stopping for 15 minutes"), and the top cold water micro-mist system is turned on simultaneously. Through physical heat exchange, the temperature difference between the center and the surface of the fermentation bed is forcibly suppressed to within 3℃, so that the core enzymatic hydrolysis temperature is stably maintained at 35±1℃, and the relative humidity of the environment is adjusted and maintained at 80%. Continuous fermentation continues until 60 hours are completed, and the fermented cooked material is obtained. S4: Transfer the fermented material directly into a hot air circulating oven, setting the inlet air temperature to 55℃ for low-temperature and gradual dehumidification. Bake for approximately 10 hours until the material moisture content drops below 8.0% before discharging. Then, use an ultra-fine pulverizer for homogenization and pulverize the entire material through an 80-mesh sieve to obtain bio-fermented raw powder rich in free chlorogenic acid. S5: Preparation of bilayer microcapsules containing sodium butyrate: A bottom-spray fluidized bed coating system was used. Hydrogenated palm oil and sunflower lecithin were mixed at a mass ratio of 5:1 and heated to 60℃ to melt into a liquid state as the inner acid-resistant wall material; a resistant starch aqueous solution was used as the outer wear-resistant wall material. Using powdered sodium butyrate as the core material, the lipid inner layer and starch outer layer were sequentially sprayed, dried, and cured to form a film, thus obtaining core-shell bilayer microcapsules. Core raw powder compounding: Accurately weigh 2.76 kg of bio-fermented raw powder and 0.24 kg of microencapsulated sodium butyrate (weight ratio 92:8), put them into a three-dimensional mixer and mix for 15 minutes to obtain a total of 3.0 kg of compound anti-inflammatory feed additive; Anti-segregation mixing (taking the preparation of 1000kg of finished product as an example): 3.0 kg of composite additive and 15.0 kg of porous aluminum silicate with a particle size of 60 mesh (weight ratio 1:5) were premixed for 5 minutes to obtain 18.0 kg of primary premix. Subsequently, the 18.0 kg of primary premix was mixed with an equal amount (18.0 kg) of ungranulated conventional corn-soybean meal basal diet base powder for 3 minutes to obtain 36.0 kg of secondary premix; then, the 36.0 kg of secondary premix was mixed with an equal amount (36.0 kg) of basal base powder for 3 minutes to obtain 72.0 kg of tertiary premix. Finally, the 72.0 kg of the three-stage premixed feed and the remaining 920.0 kg of basal diet base powder were fed into a ton-scale twin-shaft paddle mixer for total mixing. During the last two minutes of mixing on the main shaft, 8.0 kg (0.8% of the total weight) of liquid soybean oil was uniformly sprayed through the top nozzle as a vegetable oil bridge, ensuring the anti-inflammatory microparticles adhered firmly to the surface of the grain particles. The coefficient of variation for the mixing uniformity of the total mixed feed was determined to be 3.8%. S6: Pre-embedded crosslinking precursor: Just before the final discharge of the total mixture in step S5, evenly sprinkle 90g of sodium alginate and calcium carbonate composite micro powder (which is about 3% of the total weight of the anti-inflammatory additive 3kg) into the ton mixer and dry mix quickly for 1 minute. Steam-hydrothermal triggering: The total mixture is conveyed to the conditioner of the ring die granulator. Saturated high-temperature steam at a pressure of 0.3 MPa and a temperature of 80°C is introduced, and the material is kneaded and retained in the conditioner for 45 seconds. During this period, the high-temperature and high-humidity steam instantly dissolves sodium alginate. At the same time, the free chlorogenic acid inherent in the fermented powder and the free chlorogenic acid produced by microbial metabolism are used to construct a slightly acidic environment in situ in the steam condensate. This acidic environment promotes the rapid decomposition of calcium carbonate, releasing a large number of free calcium ions. The two cross-link and solidify in situ on the outer surface of the anti-inflammatory microparticles to form a calcium alginate hydrogel protective film. Pelletizing and Discharging: The softened hot material then enters the pressing chamber and is extruded into shape by a ring die with a diameter of 3.0 mm. After extrusion, the hot pellets are cooled to room temperature by a counter-flow cooling tower, thus obtaining finished broiler pellet feed with high survival rate of live bacteria and targeted release of drug efficacy. Example 2:

[0043] S1: Accurately weigh 6.0 kg of Eucommia ulmoides leaf powder and 4.0 kg of coarse coffee grounds, and dry mix them evenly to obtain 10.0 kg of mixed fermentation substrate.

[0044] S2: Bacillus subtilis was pre-cultured in a liquid medium containing 0.5% (v / v) of Eucommia ulmoides leaf decoction extract. Tea saponin powder was then mixed into the bacterial culture, making it 0.1% of the total mass of the inoculated culture. Inoculation was performed using aerosol micro-mist spraying, and water was added to adjust the total moisture content of the fermentation wet material system to 45%, with an initial viable count of 1.0 × 10⁻⁶ cells / day. 8 CFU / g.

[0045] S3: Maintain a relative humidity of 85% for the first 24 hours of solid-state fermentation; when the center temperature rises, trigger a linkage feedback regulation to maintain the center of the bed at the enzymatic hydrolysis temperature of 32℃ and the relative humidity of the environment at 75%, with a total fermentation cycle of 48 hours.

[0046] S4: The fermented material is dried at 50°C until the moisture content is less than 10%, and then pulverized to obtain biological fermentation powder.

[0047] S5: Accurately weigh 0.90 kg of fermentation powder and 0.10 kg of microencapsulated sodium butyrate, mix them evenly, and prepare 1.0 kg of compound anti-inflammatory feed additive; premix the 1.0 kg additive with 5.0 kg of porous aluminum silicate salt to eliminate static electricity and obtain 6.0 kg of primary premix. Subsequently, it was mixed into the ungranulated base powder using an equal-volume incremental process: 6.0 kg premix + 6.0 kg base powder → 12.0 kg secondary premix; 12.0 kg + 12.0 kg base powder → 24.0 kg tertiary premix; The 24.0 kg of tertiary premix and the remaining 971.0 kg of base powder were put into the mixer, and 5.0 kg of vegetable oil (0.5% of the total weight of the finished product) was sprayed in during the last 2 minutes of mixing as a liquid bridge for mixing.

[0048] S6: Before the final mixing and discharge, 20g of sodium alginate and calcium carbonate composite micro powder (accounting for 2% of the total additive weight of 1.0kg) is mixed in. Subsequently, in-situ cross-linking coating and extrusion granulation are carried out in a 75℃ steam conditioning process to obtain the finished product. Example 3:

[0049] S1: Accurately weigh 8.0 kg of Eucommia ulmoides leaf powder and 2.0 kg of coarse coffee grounds, and dry mix them evenly to obtain 10.0 kg of mixed fermentation substrate.

[0050] S2: Bacillus subtilis was pre-cultured in a liquid medium containing 1.0% (v / v) of Eucommia ulmoides leaf decoction. Tea saponin powder was then mixed into the bacterial culture, making it 0.2% of the total mass of the inoculated culture. Inoculation was performed using aerosol micro-mist spraying, and water was added to adjust the total moisture content of the fermentation wet material system to 55%, with an initial viable count of 5.0 × 10⁻⁶ cells / day. 8 CFU / g.

[0051] S3: Maintain a relative humidity of 95% for the first 24 hours of solid-state fermentation; when the center temperature rises, trigger the linkage feedback regulation to force the center of the bed to be maintained at the enzymatic hydrolysis temperature of 37℃, and the relative humidity of the environment to be maintained at 85%, with a total fermentation cycle of 72 hours.

[0052] S4: The fermented material is dried at 60°C until the moisture content is less than 10%, and then pulverized to obtain biological fermentation powder.

[0053] S5: Accurately weigh 4.75 kg of fermentation powder and mix it with 0.25 kg of microencapsulated sodium butyrate to prepare 5.0 kg of compound anti-inflammatory feed additive; premix the 5.0 kg additive with 25.0 kg of porous aluminum silicate (weight ratio 1:5) to eliminate static electricity and obtain 30.0 kg of primary premix. Subsequently, it was mixed into the ungranulated base powder using an equal-volume incremental process: 30.0 kg premix + 30.0 kg base powder → 60.0 kg secondary premix; 60.0 kg + 60.0 kg base powder → 120.0 kg tertiary premix; The 120.0 kg of grade III premix and the remaining 870.0 kg of base powder were put into the mixer, and 10.0 kg of vegetable oil (accounting for 1.0% of the total weight of the finished product) was sprayed in during the last 2 minutes of mixing as a liquid bridge for mixing.

[0054] S6: Before the final mixing and discharge, 200g of sodium alginate and calcium carbonate composite micro powder (accounting for 4% of the total additive weight of 5.0kg) is mixed in. Subsequently, in-situ cross-linking coating and extrusion granulation are carried out in an 85℃ steam conditioning process to obtain the finished product.

[0055] Comparative Example 1: The difference from Example 1 is that no coarse coffee grounds are added in S1, and 100% pure Eucommia ulmoides leaves are used for fermentation.

[0056] Comparative Example 2: The difference from Example 1 is that Bacillus subtilis in S2 is not pre-cultured with Eucommia ulmoides extract, tea saponin is not added, and it is inoculated by direct and uniform spraying with conventional water pipes (not micro-atomization).

[0057] Comparative Example 3: The difference from Example 1 is that pulse cross-flow ventilation and cold mist humidification are not activated in S3 solid-state fermentation, and passive fermentation is carried out at a constant high humidity of 90% throughout the process.

[0058] Comparative Example 4: The difference from Example 1 is that in S5, commercially available sodium butyrate coated with a single layer of common vegetable oil is used instead of the double-shelled microcapsules of the present invention.

[0059] Comparative Example 5: The difference from Example 1 is that: before mixing S6 into the basal diet pelleting, sodium alginate and calcium carbonate compound powder are not premixed, and the pelleting is directly carried out by steam conditioning at 80°C.

[0060] Comparative Example 6: The difference from Example 1 is that the microbial fermentation process of S2-S4 is not carried out. Instead, the untreated Eucommia ulmoides leaf powder, coffee grounds powder and sodium microcapsule butyrate are directly physically mixed and then granulated.

[0061] Comparative Example 7: The difference from Example 1 is that: in step S5, the mixing method of equal incremental addition is not used, but direct pouring and mixing is used.

[0062] Test Example 1: Evaluation of Fermentation Kinetics and Physicochemical Extraction Indicators; At the end of S3 fermentation, the bed porosity and peak temperature were measured. After S4 drying into powder, the viable cell count was determined by plate counting, the mass concentration of free chlorogenic acid in the fermented powder was determined by high performance liquid chromatography (HPLC), and the removal (complexation) rate of free caffeine was calculated. The test results are shown in Table 1.

[0063] Table 1. Results of fermentation physicochemical indicators and product concentration tests: As shown in Table 1, the test data revealed that in Comparative Example 1, which used pure Eucommia ulmoides leaves for fermentation, the porosity of the bed decreased sharply to 14.2% due to the swelling of Eucommia ulmoides gum after absorbing water. Insufficient air permeability severely limited the growth of aerobic bacteria, resulting in a low absolute concentration of free chlorogenic acid (850 mg / kg). In Example 1, by introducing coarse-grained coffee grounds to provide rigid support, the porosity of the fermentation system stabilized at 68.5%, promoting an increase in the concentration of free chlorogenic acid to 5,120 mg / kg. Comparative Example 3, which did not employ a combined pulse ventilation and cold mist humidification control, reached a peak fermentation pile temperature of 56.4℃, causing a significant decrease in viable bacteria count and exacerbating the thermal oxidation loss of heat-sensitive chlorogenic acid. Furthermore, in Example 1, during cell wall disruption fermentation, the released polyphenols reacted with free caffeine, resulting in a free caffeine removal rate of 95.2%; while Comparative Example 6, which did not undergo fermentation pretreatment, failed to remove free caffeine. The above data objectively verify that the physical framework and environmental regulation mechanism of the present invention can effectively improve the extraction rate of chlorogenic acid and achieve in-situ detoxification.

[0064] Test Example 2: Evaluation of formulation processing resistance and gastrointestinal targeted release; To accurately evaluate the gastrointestinal targeted release performance of microencapsulated sodium butyrate and finished feed, this invention uses the following specific in vitro simulated poultry digestive tract model for parameter determination: (1) Preparation and testing of simulated poultry gizzard fluid (including mechanical grinding conditions): The pH of the buffer solution was adjusted to 2.0 using 0.1 mol / L dilute hydrochloric acid, and pepsin (enzyme activity ≥3000 U / g) was added to prepare a 10 mg / mL liquid chromatography buffer. 2.0 g of the finished product containing microencapsulated sodium butyrate granules was accurately weighed and placed in a conical flask containing 50 mL of the simulated gizzard fluid. To simulate the intense physical grinding environment unique to avian gizzards, 10.0 g of acid-washed and purified quartz sand (particle size limited to 1.0~2.0 mm) was added to the flask. The conical flask was then placed in a 41°C water bath shaker and ground at 150 rpm for 2 hours. The supernatant was collected by centrifugation, and the concentration of free butyrate was determined by gas chromatography to calculate the leakage rate.

[0065] (2) Preparation and testing of simulated posterior intestinal fluid: Prepare 0.1 mol / L phosphate-buffered saline (PBS) and adjust the pH to 6.8. Add avian complex bile salts (mainly containing sodium taurine chenodeoxycholate) to a final concentration of 3.0 mg / mL, and add pancreatic lipase (enzyme activity ≥4000 U / g) to a final concentration of 5.0 mg / mL. Mix well and set aside. Filter and wash the digested residue from the above-mentioned gizzard fluid, and transfer it to an Erlenmeyer flask containing 50 mL of the simulated posterior intestinal fluid. Continue the reaction at a constant temperature of 41°C and 100 rpm for 4 hours with gentle shaking. Take the supernatant to determine the concentration of free butyrate and calculate the final release rate.

[0066] Table 2. Results of Granulation Tolerability and Gastrointestinal Site-Specific Release Performance Tests: As shown in Table 2, the survival rate of viable bacteria in Comparative Example 5, which did not use premixed calcium alginate precursor, decreased significantly to 28.4% after undergoing high-temperature steam conditioning at 80℃ and mechanical extrusion granulation. In contrast, Example 1 utilized the steam hydrothermal conditions inherent in the granulation process to in-situ trigger the formation of a calcium alginate hydrogel membrane, which provided good buffering protection against heat transfer and mechanical shear force, maintaining a viable bacteria survival rate of 88.5% after granulation. In the simulated digestive fluid test, Comparative Example 4, using conventional single-layer vegetable oil coating of sodium butyrate, was unable to withstand the strong physical grinding of the gizzard segment, resulting in a simulated gastric juice leakage rate as high as 58.6%. Example 1, using a double-shell microcapsule structure composed of resistant starch and high-melting-point lipids, effectively controlled the leakage rate of the simulated gizzard segment to 6.5%, and achieved a release rate of 94.2% in the simulated mid-to-late intestinal fluid, demonstrating superior foregut resistance and targeted release performance in the posterior intestinal tract. Meanwhile, through the aluminum silicate premixing and liquid bridge bonding process, the coefficient of variation of the mixing uniformity of the finished product in Example 1 was controlled at an ideal 3.8%.

[0067] Test Example 3: Verification Experiment of Anti-inflammatory and Growth-promoting Effects in Broilers; 1200 one-day-old broiler chickens were randomly divided into 4 groups. The test diets were: control group (fed only the basal diet), Example 1 group, Comparative Example 4 group (gastric leakage group), and Comparative Example 6 group (undetoxified mixed group). On day 14, mild necrotizing enteritis (NE) stress was induced in the entire flock by inoculating with Clostridium perfringens in drinking water. Production performance and intestinal mucosal morphology scores (cecal lesion score: 0 points for healthy, 4 points for severe necrosis) were recorded for 42 days. The results are shown in Table 3.

[0068] Table 3. Live feeding performance and intestinal health characteristics of broiler chickens at 42 days: Table 3 shows the data from the live feeding experiment. Comparative Example 6, which directly added untreated coffee grounds substrate, retained a high concentration of free caffeine, triggering an adverse anti-nutritional neurological response in broilers. This resulted in a significant decrease in the average daily feed intake and daily weight gain, and an increase in the feed conversion ratio to 2.09. In contrast, Example 1 showed normal feed intake and growth indicators, confirming the effectiveness of the fermentation and detoxification process in ensuring feed palatability and biosafety. In the intervention for enteritis, Comparative Example 4 experienced significant leakage of sodium butyrate in the stomach, failing to reach the cecal region adequately for mucosal repair, leading to a high cecal lesion score (2.15). In contrast, Example 1, through the combined synergistic effect of front-end free chlorogenic acid inhibition and back-end microencapsulated butyrate targeted repair, significantly reduced the cecal lesion score caused by necrotizing enteritis to 0.42, optimizing the feed conversion ratio to 1.57. The above animal experimental data objectively demonstrate that the compound anti-inflammatory preparation of this invention can effectively maintain the intestinal health of broilers and genuinely improve animal production performance.

[0069] The embodiments of the present invention have been described above. However, the present invention is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of the present invention without departing from the spirit and scope of the claims. All of these forms are within the protection scope of the present invention.

Claims

1. A method for preparing broiler feed based on a compound of anti-inflammatory plant extracts, characterized in that: Includes the following steps: S1: Pulverize dried Eucommia ulmoides leaves to obtain Eucommia ulmoides leaf powder; pulverize coffee grounds and pass them through a 20-40 mesh sieve to obtain coarse-grained coffee grounds; then mix the Eucommia ulmoides leaf powder and the coarse-grained coffee grounds in a weight ratio of 6:4-8:2 to form a mixed fermentation substrate; S2: Add pretreated Bacillus subtilis solution to the mixed fermentation substrate, and add water to adjust the water content of the system to 45% to 55% to obtain fermented wet material; S3: Place the fermented wet material in an aerobic environment for solid-state aerobic fermentation, control the fermentation temperature at 32℃~37℃, and the fermentation cycle at 48~72 hours to obtain fermented cooked material; S4: The fermented material is directly dried at low temperature, with the drying temperature controlled at 50℃~60℃, until the moisture content is less than 10%, and then homogenized and pulverized to obtain bio-fermented raw powder rich in free chlorogenic acid. S5: The bio-fermented raw powder and microencapsulated sodium butyrate are mixed evenly at a weight ratio of 90:10 to 95:5 to obtain a compound anti-inflammatory feed additive, which is then added to the basal diet of broilers for mixing and pelleting. In step S2, the pretreatment of the Bacillus subtilis solution includes: first, incubating the solution in a liquid culture medium containing 0.5%–1.0% Eucommia ulmoides leaf decoction for 6–8 hours until the logarithmic growth phase; then, mixing tea saponin into the Bacillus subtilis solution to achieve a tea saponin concentration of 0.1%–0.2%. The pretreated Bacillus subtilis solution was introduced into the mixed fermentation substrate via aerosol micro-mist spraying, with an initial viable count of 1.0 × 10⁻⁶ bacteria per gram of wet fermentation material. 8 ~5.0×10 8 CFU; In step S3, the solid-state aerobic fermentation adopts a staged temperature and humidity feedback control method: the relative humidity of the environment is maintained at 85% to 95% for the first 24 hours of fermentation; when the temperature at the center of the fermentation bed rises to 36°C, pulsed cross-flow ventilation and cold mist humidification are activated to allow the airflow to diffuse along the internal pore channels formed by the coarse-grained coffee grounds, so as to control the temperature difference between the center and the surface of the fermentation bed to not exceed 3°C, and maintain the fermentation bed in the enzymatic hydrolysis temperature range of 32°C to 37°C; at the same time, the relative humidity of the fermentation bed environment is maintained in the range of 75% to 85%.

2. The method for preparing broiler feed based on anti-inflammatory plant extracts according to claim 1, characterized in that: In step S5, before adding the compound anti-inflammatory feed additive to the broiler basal diet for mixing and pelleting, 2% to 4% of sodium alginate and calcium carbonate composite micro powder by weight is premixed into the compound anti-inflammatory feed additive. In the subsequent pelleting process, a steam conditioning treatment at 75°C to 85°C is performed to dissolve the free chlorogenic acid contained in the fermented raw powder using steam moisture to form a slightly acidic environment, thereby triggering the cross-linking reaction between sodium alginate and calcium ions. A calcium alginate protective layer is formed in situ on the outer surface of the compound anti-inflammatory feed additive particles to reduce the thermal and mechanical damage to the live bacteria, chlorogenic acid, and microencapsulated sodium butyrate encapsulation structure in the fermented raw powder during the conditioning and pelleting process.

3. The method for preparing broiler feed based on anti-inflammatory plant extracts according to claim 2, characterized in that: The compound anti-inflammatory feed additive accounts for 0.1% to 0.5% of the total mass of the final broiler feed. Furthermore, when the compound anti-inflammatory feed additive is mixed into the basal diet of broilers, a step-by-step premixing and liquid bridge adhesion process is adopted: firstly, the compound anti-inflammatory feed additive and porous aluminum silicate with a particle size of 60-80 mesh are premixed at a weight ratio of 1:

5. Then, an equal-volume incremental mixing process is used to gradually mix it into the basal diet powder. Finally, 0.5% to 1.0% of vegetable oil by weight of the total basal diet is sprayed in as a liquid bridge binder for total mixing, so that the micro powder of the compound anti-inflammatory feed additive adheres to the surface of the ungranulated coarse powder of the basal diet, and the coefficient of variation of the uniformity of the additive distribution in the final broiler feed is not greater than 5%.

4. The method for preparing broiler feed based on anti-inflammatory plant extracts according to claim 3, characterized in that: In step S5, the microencapsulated sodium butyrate has a core-shell bilayer structure, with an outer wall material of resistant starch and an inner wall material of a lipid layer of hydrogenated palm oil and sunflower lecithin melted together at a mass ratio of 4:1 to 6:1 with a melting point of 50℃ to 60℃. The leakage rate of the microencapsulated sodium butyrate is less than 10% after 2 hours in simulated avian gizzard fluid with pH ≤ 3.0 and mechanical grinding, while the release rate is greater than 90% in simulated posterior intestinal fluid rich in specific bile salts and pancreatic lipase with a pH of 6.5 to 7.

5.

5. A broiler feed based on an anti-inflammatory plant extract compound, prepared using the method described in any one of claims 2-4; characterized in that: The broiler feed includes a basal broiler diet and a compound anti-inflammatory feed additive accounting for 0.1% to 0.5% of the total mass of the finished product; The compound anti-inflammatory feed additive includes: coarse-grained coffee grounds carrier, Eucommia ulmoides leaf fermentation components attached to the surface and pores of the carrier, Bacillus subtilis dormant spores dispersed in the fermentation components, and microencapsulated sodium butyrate; the compound anti-inflammatory feed additive is coated with a calcium alginate protective layer.

6. The broiler feed based on anti-inflammatory plant extracts as described in claim 5, characterized in that, The broiler feed includes a basal broiler diet and a compound anti-inflammatory feed additive dispersed on its surface. The compound anti-inflammatory feed additive uses coarse-grained coffee grounds with an internal porous physical framework as a carrier. On the surface and in the pores of the carrier, there are Eucommia ulmoides leaf fermentation components, free chlorogenic acid, Bacillus subtilis dormant spores, and microencapsulated sodium butyrate, and the outside is coated with the calcium alginate protective layer.

7. The broiler feed based on anti-inflammatory plant extracts according to claim 6, characterized in that: The compound anti-inflammatory feed additive is fixedly attached to the surface of the broiler basal diet pellets in the form of compound anti-inflammatory granules. The outer surface of the compound anti-inflammatory granules is coated with a calcium alginate protective layer, and the coefficient of variation of the distribution and mixing uniformity of the compound anti-inflammatory feed additive in the finished broiler feed is not greater than 5%.

8. The broiler feed based on anti-inflammatory plant extracts according to claim 7, characterized in that: The finished broiler feed pellets contain at least 4 mg / kg of free chlorogenic acid and at least 2.0 × 10⁻⁶ dormant Bacillus subtilis. 6 CFU / g.

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