Rubber tree wood chip cultivation feed and artificial feeding method for a rubber material bark beetle

Abstract

The present application relates to the technical field of artificial feeding of small insects, in particular to rubberwood small insects rubber tree wood chip cultivation feed and artificial feeding method. The rubberwood small insect cultivation feed comprises yeast powder, weiss salt, casein, starch, sucrose, agar powder, streptomycin sulfate, wheat germ oil, anhydrous ethanol, rubber tree wood chip and deionized water. The rubberwood small insect feeding method comprises the following steps: obtaining rubberwood small insect female adults; inoculating rubberwood small insect female adults; and feeding rubberwood small insects in a climate box. The artificial feeding method of rubberwood small insects provided by the present application can obtain stable test insect sources, provide insect source guarantee for studying the biological and ecological characteristics of rubberwood small insects, and further lay a theoretical foundation for formulating rubberwood small insect prevention and control measures.

Description

Technical Field

[0001] This invention relates to the field of artificial breeding technology for bark beetles, and in particular to a rubber tree sawdust-based feed and artificial breeding method for the rubber tree bark beetle. Background Technology

[0002] The rubberwood beetle (Xyleborus affinis Eichhoff) belongs to the genus Xyleborus in the family Curculionidae of the order Coleoptera. It is a wood-boring pest. The rubberwood beetle lives and reproduces within the xylem of host trees, feeding on fungi it carries and cultivates within the xylem of the host tree. Because it feeds on fungi rather than the tissues of the host plant, it is not strictly specific to any particular host tree and can damage more than 200 economic crops, including rubber trees (Hevea brasiliensis), avocados (Persea americana), and cocoa (Theobroma cacao). In rubber-growing areas of Hainan and Yunnan provinces in my country, the rubberwood beetle is widely distributed and frequently causes outbreaks, posing a significant threat to the stable and secure supply of natural rubber in my country. Furthermore, the rubber bark beetle possesses a haploid-diploid and highly inbred mating system. Upon arriving at a new location, even unmated female adults can reproduce and establish a population as long as they carry worm tunnel fungi. This unique genetic pattern makes outbreaks of the rubber bark beetle extremely difficult to control.

[0003] The unique biological and ecological characteristics of the rubberwood beetle are difficult to obtain through forest observation. Indoor rearing allows for detailed observation of its reproductive, growth, and developmental biological characteristics, as well as research on its nutrient utilization methods when feeding on fungi. Furthermore, large-scale indoor rearing of the rubberwood beetle enables toxicological and chemical ecology studies, exploring precise and efficient chemical agents and methods of action for its control, and identifying chemical signaling substances used in physicochemical induction, thus laying a theoretical foundation for its control. Current research on beetle rearing primarily focuses on the bark beetle, including log rearing, phloem tissue rearing, and artificial feed rearing. However, reports on rearing methods for the wood-boring beetle are scarce. In foreign studies, artificial feeds for the rubberwood beetle use sawdust from beech and oak trees; there are currently no feed formulations using rubberwood sawdust or laboratory artificial rearing methods. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides a rubber tree bark sawdust-based feed and an artificial rearing method for the rubber tree bark beetle. This feed is easy to prepare, and the rearing method is simple and convenient, enabling continuous indoor rearing of the rubber tree bark beetle. It can also serve as a reference for the artificial rearing of other wood-boring insects.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] This invention provides a rubber tree sawdust-based feed for the rubber bark beetle, comprising the following components in parts by weight: 20 parts agar powder, 5 parts yeast powder, 0.5 parts Wiener salt, 5 parts casein, 5 parts starch, 10 parts sucrose, 0.35 parts streptomycin sulfate, 2.5 parts wheat germ oil, 5 parts anhydrous ethanol, 150 parts rubber tree sawdust, and 350 parts deionized water.

[0007] The present invention also provides a feed tube for rubber bark beetles, the preparation method of which includes the following steps:

[0008] 1) Dissolve the agar powder, yeast powder, Wiegand salt, casein, starch, sucrose, and streptomycin sulfate in the rubber tree sawdust cultivation feed described in the above technical solution in deionized water to obtain a solution;

[0009] 2) Heat the solution obtained in step 1) to a paste state, and then mix it with wheat germ oil, anhydrous ethanol and rubber wood chips in the rubber wood chip culture feed of claim 1 to obtain the rubber wood chip culture feed.

[0010] 3) Place the rubber tree sawdust culture feed described in step 2) into a flat-bottomed centrifuge tube, seal and sterilize it, add wax liquid into the flat-bottomed centrifuge tube to seal the surface of the rubber tree sawdust culture feed, and seal it with the centrifuge tube cap to obtain the feed tube.

[0011] Preferably, in step 3), the amount of rubber tree wood chips used to cultivate the feed is half the capacity of the flat-bottomed centrifuge tube;

[0012] The flat-bottomed centrifuge tube has a capacity of 50 mL, a diameter of 28.8 mm, and a height of 117.3 mm.

[0013] Use breathable sealing film to seal.

[0014] Preferably, the sterilization conditions in step 3) include sterilization for 20 minutes at a temperature of 121°C and a pressure of 0.1 MPa.

[0015] This invention also provides a method for artificially raising the rubber bark beetle, comprising the following steps:

[0016] A. Drill holes in the surface of the rubber tree wood chips used to cultivate feed in the feed tube described in the above technical solution;

[0017] B. Place the female adult rubber beetle in a feed tube and raise them until the offspring adults emerge.

[0018] Preferably, the diameter of the hole is 1~1.5mm and the depth is 5~8mm, with one hole drilled for each feed tube.

[0019] Preferably, the female adult insect is disinfected and then placed in a feed tube. The disinfection method includes: disinfecting with a 75% ethanol solution for 10 seconds, rinsing twice with sterile water for 10 seconds each time, and finally absorbing the moisture from the surface of the female adult insect with sterile filter paper.

[0020] Preferably, the rearing conditions include a temperature of 25-27°C and a relative humidity of 45-55%.

[0021] Preferably, once the rubber bark beetle has completely burrowed into the feed and excreted its frass, the frass is cleaned up. Then, the frass excreted by the rubber bark beetle in the feed tube is cleaned up every 7 days until the adult offspring emerge.

[0022] The beneficial effects of this invention are:

[0023] The present invention provides a complete and comprehensive method for preparing artificial feed for rubber bark beetles using rubber tree wood chips. The artificial rearing operation is simple, convenient, and easy to implement. It can directly feed female adult rubber bark beetles captured in the wild with artificial feed to raise offspring, quickly establish laboratory populations, and save a lot of manpower and resources required for collecting and testing rubber bark beetles. It provides a large source of insects for the biological and ecological research of rubber bark beetles and lays the foundation for further understanding and control of rubber bark beetles. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0025] Figure 1 Evaluation results of biological parameters of rubber bark beetle on the cultivation feed formulated for different types of sawdust;

[0026] Figure 2 Evaluation results of biological parameters of rubber tree bark beetle by cultivation feeds formulated with different amounts of rubber tree wood chips. Detailed Implementation

[0027] This invention provides a rubber tree bark beetle cultivation feed made from rubber tree sawdust, comprising the following components in parts by weight: 20 parts agar powder, 5 parts yeast powder, 0.5 parts Webster's salt, 5 parts casein, 5 parts starch, 10 parts sucrose, 0.35 parts streptomycin sulfate, 2.5 parts wheat germ oil, 5 parts anhydrous ethanol, 150 parts rubber tree sawdust, and 350 parts deionized water. This invention does not have specific limitations on the source of the above chemical reagents; commercially available products can be used. In this invention, the yeast powder (Oxoid), Webster's salt (MP Biomedicals), casein (Shanghai Aladdin Biochemical Technology Co., Ltd.), starch (Shanghai Aladdin Biochemical Technology Co., Ltd.), sucrose (Xilong Scientific), agar powder (BioFroxx), streptomycin sulfate (BioFroxx), wheat germ oil (Shanghai Aladdin Biochemical Technology Co., Ltd.), and anhydrous ethanol (Xilong Scientific, purity >99.7%) are used. In this invention, the method for preparing rubber tree wood chips includes the following steps: collecting freshly broken branches of rubber trees that are free from pests and diseases in the wild, sawing them into wood chips about 1 cm thick, placing them in an 80 ℃ oven to dry for 24 h, removing the phloem tissue on the outer edge of the wood chips, crushing them into wood chips using a wood shredder, and passing them through a 30-mesh stainless steel filter to obtain rubber tree wood chips.

[0028] This invention provides a feed tube for rubber bark beetles, and the preparation method of the feed tube includes the following steps:

[0029] 1) Dissolve the agar powder, yeast powder, Wiegand salt, casein, starch, sucrose, and streptomycin sulfate in the rubber tree sawdust cultivation feed described in the above technical solution in deionized water to obtain a solution;

[0030] 2) Heat the solution obtained in step 1) to a paste state, and then mix it with wheat germ oil, anhydrous ethanol and rubber wood chips in the rubber wood chip culture feed of claim 1 to obtain the rubber wood chip culture feed.

[0031] 3) Place the rubber tree sawdust culture feed described in step 2) into a flat-bottomed centrifuge tube, seal and sterilize it, add wax liquid into the flat-bottomed centrifuge tube to seal the surface of the rubber tree sawdust culture feed, and seal it with the centrifuge tube cap to obtain the feed tube.

[0032] In this invention, the amount of rubber tree sawdust used to cultivate the feed is half the capacity of the flat-bottomed centrifuge tube. Preferably, the flat-bottomed centrifuge tube has a capacity of 50 mL, a diameter of 28.8 mm, and a height of 117.3 mm. A breathable sealing film is preferably used for sealing. The sterilization conditions preferably include sterilization at 121°C and 0.1 MPa for 20 minutes.

[0033] This invention also provides a method for artificially raising the rubber bark beetle, comprising the following steps:

[0034] A. Drill holes in the surface of the rubber tree wood chips used to cultivate feed in the feed tube described in the above technical solution;

[0035] B. Place the female adult rubber beetle in a feed tube and raise them until the offspring adults emerge.

[0036] In this invention, the diameter of the hole is preferably 1-1.5 mm, the depth is preferably 5-8 mm, and one hole is preferably made in each feed tube. This invention preferably uses sterilized iron wire for punching the holes.

[0037] In this invention, the female adult insects are preferably disinfected and then placed in a feed tube. The disinfection method preferably includes: disinfecting with a 75% ethanol solution for 10 seconds, rinsing twice with sterile water for 10 seconds each time, and finally blotting the surface moisture of the female adult insects with sterile filter paper. In this invention, the preferred rearing conditions include: a temperature of 25-27°C and a relative humidity of 45-55%.

[0038] In this invention, after observing that the rubber bark beetle has completely burrowed into the feed and excreted its frass, the frass is cleaned up. Then, the frass excreted by the rubber bark beetle in the feed tube is cleaned up every 7 days until the offspring adults emerge and burrow out.

[0039] To further illustrate the present invention, the following detailed description is provided in conjunction with embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0040] Example 1

[0041] Evaluation results of biological parameters of rubber bark beetle by different types of sawdust-based breeding feeds.

[0042] The breeding feed for rubber bark beetles prepared in this embodiment uses commercially available yeast powder, Wiener salt, casein, starch, sucrose, agar powder, streptomycin sulfate, wheat germ oil, and anhydrous ethanol. Poplar and pine sawdust are also commercially available, while rubber tree sawdust is homemade.

[0043] The following four treatments are prepared according to the testing requirements.

[0044] Treatment 1: 20 g agar powder, 5 g yeast powder, 0.5 g Wiener salt, 5 g casein, 5 g starch, 10 g sucrose, 0.35 g streptomycin sulfate, 2.5 g wheat germ oil, 5 g anhydrous ethanol, 200 g poplar sawdust, 350 mL deionized water;

[0045] Treatment 2: 20 g agar powder, 5 g yeast powder, 0.5 g Wiener salt, 5 g casein, 5 g starch, 10 g sucrose, 0.35 g streptomycin sulfate, 2.5 g wheat germ oil, 5 g anhydrous ethanol, 200 g pine sawdust, and 350 mL deionized water;

[0046] Treatment 3: 20 g agar powder, 5 g yeast powder, 0.5 g Wiener salt, 5 g casein, 5 g starch, 10 g sucrose, 0.35 g streptomycin sulfate, 2.5 g wheat germ oil, 5 g anhydrous ethanol, 200 g rubber tree wood chips, and 350 mL deionized water;

[0047] Treatment 4: 20 g agar powder, 5 g yeast powder, 0.5 g Werner salt, 5 g casein, 5 g starch, 10 g sucrose, 0.35 g streptomycin sulfate, 2.5 g wheat germ oil, and 5 g anhydrous ethanol.

[0048] The rubber tree sawdust culture feed prepared in the application example is used for the artificial rearing of rubber tree bark beetles. The specific steps are as follows:

[0049] (1) Mix yeast powder, Wiener salt, casein, starch, sucrose, agar powder, streptomycin sulfate, wheat germ oil, anhydrous ethanol, sawdust, and deionized water according to the above-mentioned usage amounts, heat and stir evenly, sterilize under high temperature and high pressure, package and compact, wipe with water and seal with wax to make a breeding feed. The specific steps are as follows:

[0050] a. First, dissolve agar powder, yeast powder, Wiegand salt, casein, starch, sucrose, and streptomycin sulfate in deionized water according to their components, stir until completely dissolved, heat over low heat on an induction cooker until it becomes a paste, then add wheat germ oil and anhydrous ethanol, stir until evenly mixed, then add sawdust and stir until well mixed to form a mixture; among them, poplar sawdust in treatment 1 and pine sawdust in treatment 2 are commercially available, and are used after passing through a 30-mesh stainless steel filter; rubber tree sawdust in treatment 3 is obtained by collecting freshly broken branches of rubber trees in the wild that are free from disease and pests, sawing them into wood chips about 1 cm thick, placing them in an 80 ℃ oven to dry for 24 h, removing the bast tissue on the outer edge of the wood chips, crushing them into sawdust using a wood shredder, and passing them through a 30-mesh stainless steel filter to obtain rubber tree sawdust;

[0051] b. Dispense the above mixture into 50 mL flat-bottom centrifuge tubes (the capacity of the bottom centrifuge tube is 50 mL, the diameter is 28.8 mm, and the height is 117.3 mm). Compact 25 mL of the mixture into each centrifuge tube and seal the centrifuge tube opening with breathable sealing film.

[0052] c. Sterilize the 50 mL flat-bottomed centrifuge tubes filled with feed (hereinafter referred to as feed tubes) with steam at 121℃ and 0.1MPa for 20 min;

[0053] d. After the feed tube has cooled to room temperature, wipe the moisture off the side wall of the sterilized feed tube with sterile defatted cotton in a clean bench, and seal the feed tube with a sterile 50 mL centrifuge tube cap.

[0054] e. Heat and melt medical paraffin wax, add the wax liquid to the feed tube, shake the feed tube quickly to completely seal the surface of the feed with the wax liquid, seal the feed tube with a 50 mL centrifuge tube cap, and store it in a refrigerator at 4 ℃ for later use.

[0055] (2) Indoor artificial rearing of the rubber tree bark beetle:

[0056] In October 2025, bark beetle traps were set up at the Seventh Team of the Natural Rubber Experimental Farm of the Two Academies in Danzhou City, Hainan Province. Adult bark beetles flying in the rubber forest were collected and brought back to the laboratory of the Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou City, Hainan Province. Artificial rearing experiments of bark beetles were carried out from October to November 2025. The specific steps are as follows:

[0057] a. Collect adult female rubber tree beetles in the wild, and select those that are intact and active to bring back to the laboratory;

[0058] b. Disinfect the collected adult female rubber beetles with 75% alcohol for 10 seconds, then rinse them twice with sterile water for 10 seconds each time, and use sterile filter paper to absorb the moisture on the surface of the beetles.

[0059] c. In the clean bench, sterilize a 1.5 mm diameter iron wire with an alcohol lamp, and use the iron wire to make a hole in the prepared feed, one hole with a depth of 5 mm.

[0060] d. Gently transfer the bark beetle into the feed tube using a brush sterilized with 75% alcohol. Inoculate one adult female bark beetle into each feed tube and loosely seal the feed tube with a 50 mL centrifuge tube cap.

[0061] e. Place the feed tube in a climate chamber with a temperature of (26±1)℃ and a relative humidity of (50±5)% for dark rearing. After observing that the rubber beetle has completely burrowed into the feed and excreted its frass (approximately 1-2 days after inoculation of the beetle), use a brush to clean the frass from the feed tube. Then, clean the frass excreted by the rubber beetle in the feed tube every 7 days or so until the offspring adults emerge.

[0062] Based on the above-described artificial rearing methods, the biological parameters of the rubber tree bark beetle used for indoor artificial rearing were evaluated. Specific results are shown in [link to results]. Figure 1In this experiment, each treatment was replicated 10 times. One *Bartholin's sap borer* was inoculated into each feed tube, counted as one replicate. The total number of *Bartholin's sap borer* that emerged from the feed tube and the survival rate were recorded from the time the first adult offspring emerged until no more adults emerged. Five female adult offspring were randomly selected from each feed tube and weighed. Additionally, ten female adult *Bartholin's sap borers* captured in the wild were randomly selected and weighed to compare the weight difference between the indoor and wild populations.

[0063] from Figure 1 It can be seen that treatment 4 failed to produce offspring of *Bark Borer rubrum*, while treatments 1, 2, and 3 all produced adult offspring. Regarding the number of adult offspring produced per female, treatment 3 produced the highest total number of adult offspring per female, averaging 31.50, significantly higher than treatments 1 and 2. Regarding the survival rate of adult offspring, treatment 3 had an average survival rate of 92.48%, significantly higher than treatments 1 and 2. Regarding the weight of adult offspring, comparing the weights of adult offspring obtained from treatments 1, 2, and 3 with those collected from the wild, the weights of adult offspring obtained from treatment 3 and those collected from the wild were 84.90 mg / offspring and 85.80 mg / offspring, respectively. There was no significant difference between the two, but both were significantly higher than those from treatments 1 and 2. These results indicate that treatment 3 produced the best biological performance in the adult offspring of *Bark Borer rubrum*, meaning that *Bark Borer rubrum* can be reared indoors using rubber tree sawdust.

[0064] Example 2

[0065] Evaluation results of biological parameters of rubber tree bark beetle by cultivation feeds formulated with different amounts of rubber tree wood chips.

[0066] In Example 1, rubber tree sawdust was selected as the suitable cultivation sawdust for establishing an indoor population of the rubberwood beetle. To investigate the appropriate amount of rubber tree sawdust, different amounts of rubber tree sawdust were weighed and used to prepare cultivation feed, and the effects of cultivation feed prepared with different amounts of rubber tree sawdust on the biological parameters of the rubberwood beetle were determined.

[0067] The rubber tree sawdust culture feed for the rubber bark beetle prepared in this embodiment uses commercially available yeast powder, Wiener salt, casein, starch, sucrose, agar powder, streptomycin sulfate, wheat germ oil, and anhydrous ethanol. The method for obtaining the rubber tree sawdust is the same as in Example 1.

[0068] The following four treatments are prepared according to the testing requirements.

[0069] Treatment 1: 20 g agar powder, 5 g yeast powder, 0.5 g Wiener salt, 5 g casein, 5 g starch, 10 g sucrose, 0.35 g streptomycin sulfate, 2.5 g wheat germ oil, 5 g anhydrous ethanol, 100 g rubber tree wood chips, and 350 mL deionized water;

[0070] Treatment 2: 20 g agar powder, 5 g yeast powder, 0.5 g Wiener salt, 5 g casein, 5 g starch, 10 g sucrose, 0.35 g streptomycin sulfate, 2.5 g wheat germ oil, 5 g anhydrous ethanol, 150 g rubber tree wood chips, and 350 mL deionized water;

[0071] Treatment 3: 20 g agar powder, 5 g yeast powder, 0.5 g Wiener salt, 5 g casein, 5 g starch, 10 g sucrose, 0.35 g streptomycin sulfate, 2.5 g wheat germ oil, 5 g anhydrous ethanol, 200 g rubber tree wood chips, and 350 mL deionized water;

[0072] Treatment 4: 20 g agar powder, 5 g yeast powder, 0.5 g Wiener salt, 5 g casein, 5 g starch, 10 g sucrose, 0.35 g streptomycin sulfate, 2.5 g wheat germ oil, 5 g anhydrous ethanol, 250 g rubber tree wood chips, and 350 mL deionized water;

[0073] In the application examples, the preparation method of rubber tree sawdust culture feed, the acquisition of female adult rubber bark beetles, and the artificial rearing steps are the same as in Example 1.

[0074] Following the specific implementation steps in Example 1, from December 2025 to January 2026, the biological parameters of the rubber tree bark beetle were evaluated using cultivation feeds prepared with different amounts of rubber tree sawdust. Specific results are shown in [link to example]. Figure 2 In this experiment, each treatment was replicated 10 times. One *Bartholin's sap borer* was inoculated into each feed tube, counted as one replicate. The total number of *Bartholin's sap borer* that emerged from the feed tube and the survival rate were recorded from the time the first adult offspring emerged until no more adults emerged. Five female adult offspring were randomly selected from each feed tube and weighed. Additionally, ten female adult *Bartholin's sap borers* captured in the wild were randomly selected and weighed to compare the weight difference between the indoor and wild populations.

[0075] from Figure 2It can be seen that, regarding the number of progeny adults produced by a single female adult, treatment 2 produced the most, reaching 33.80 individuals, with no significant difference compared to treatment 3. However, the numbers from treatments 2 and 3 were significantly higher than those from treatments 1 and 4. Regarding the survival rate of progeny adults, treatment 2 had the highest rate at 93.88%, followed by treatment 3, but the difference between treatment 3 and treatments 2 and 4 was not significant. Treatment 1 had the lowest rate at 68.12%, significantly lower than the other three treatments. Regarding the weight of the obtained progeny female adults, treatment 2 had the highest rate at 84.60 mg / adult. The weight of progeny female adults from treatments 2 and 3 was not significantly different from that of wild-collected female adults, but the weight of progeny female adults from treatments 1 and 4 was significantly lower than that of wild-collected female adults. In summary, the progeny adults produced from *Bark Beetle* reared in treatment 2 exhibited the best biological performance and can be used for indoor rearing.

[0076] Example 3

[0077] Evaluation results of biological parameters of F1 generation of rubber tree bark beetle on rubber tree sawdust-cultured feed.

[0078] In Example 2, the appropriate amount of rubber tree sawdust in the culture feed for establishing an indoor population of *Bartholin's sapwood beetle* was further screened. To investigate the effect of the screened rubber tree sawdust culture feed components on the subculture of *Bartholin's sapwood beetle*, female adults of the F1 generation of *Bartholin's sapwood beetle* obtained from treatment 2 in Example 2 were inoculated into the culture feed, and the biological parameters of the F1 generation of *Bartholin's sapwood beetle* on the culture feed were measured.

[0079] The rubber tree sawdust culture feed for the rubber bark beetle prepared in this embodiment uses commercially available yeast powder, Wiener salt, casein, starch, sucrose, agar powder, streptomycin sulfate, wheat germ oil, and anhydrous ethanol.

[0080] Prepare one treatment as required for testing.

[0081] Treatment 1: 20 g agar powder, 5 g yeast powder, 0.5 g Wechsler salt, 5 g casein, 5 g starch, 10 g sucrose, 0.35 g streptomycin sulfate, 2.5 g wheat germ oil, 5 g anhydrous ethanol, 150 g rubber tree wood chips, and 350 mL deionized water;

[0082] In the application examples, the preparation method of rubber tree sawdust culture feed and the artificial breeding steps of rubber bark beetles are the same as in Example 1.

[0083] Following the specific implementation steps in Example 1, an artificial rearing experiment of the F1 generation of *Bark Beetle* was conducted from February to March 2026. The specific results are shown in Table 1. Each treatment was replicated 10 times. One *Bark Beetle* was inoculated into each feed tube, counted as one replicate. From the moment the first adult offspring emerged from the feed until no more adult offspring emerged, the total number of *Bark Beetles* emerging from the feed, the survival rate, and the sex ratio were recorded. Five adult offspring were randomly selected from each feed tube and weighed.

[0084] Table 1 shows that the total number of adult progeny produced by the F1 generation of *Bark Beetle* on the culture feed was 35.00±1.56, with a survival rate of 93.13±0.82%. The sex ratio (female:male) was 38.50:1, and the weight of a single female adult progeny was 84.94±1.02 mg, which was not significantly different from treatment 3 in Example 1. These results indicate that *Bark Beetle* can complete its subculture on the selected rubber tree sawdust culture feed, and the adult progeny exhibits good biological performance.

[0085] Table 1. Biological parameters of the F1 generation of *Bark Beetle of Rubber* on rubber tree sawdust culture feed.

[0086]

[0087] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A rubber tree sawdust-based feed for cultivating the rubber bark beetle, characterized in that, The components include the following parts by weight: 20 parts agar powder, 5 parts yeast powder, 0.5 parts Wiener salt, 5 parts casein, 5 parts starch, 10 parts sucrose, 0.35 parts streptomycin sulfate, 2.5 parts wheat germ oil, 5 parts anhydrous ethanol, 150 parts rubber tree wood chips, and 350 parts deionized water.

2. A feed tube for rubber bark beetles, characterized in that, The method for preparing the feed tube includes the following steps: 1) Dissolve the agar powder, yeast powder, Wiener salt, casein, starch, sucrose, and streptomycin sulfate in the rubber tree wood sawdust cultivation feed according to claim 1 in deionized water to obtain a solution; 2) Heat the solution obtained in step 1) to a paste state, and then mix it with wheat germ oil, anhydrous ethanol and rubber wood chips in the rubber wood chip culture feed of claim 1 to obtain the rubber wood chip culture feed. 3) Place the rubber tree sawdust culture feed described in step 2) into a flat-bottomed centrifuge tube, seal and sterilize it, add wax liquid into the flat-bottomed centrifuge tube to seal the surface of the rubber tree sawdust culture feed, and seal it with the centrifuge tube cap to obtain the feed tube.

3. The feed tube according to claim 2, characterized in that, Step 3) The amount of rubber tree wood chips used to cultivate the feed is half the capacity of the flat-bottomed centrifuge tube; The flat-bottomed centrifuge tube has a capacity of 50 mL, a diameter of 28.8 mm, and a height of 117.3 mm. Use breathable sealing film to seal.

4. The feed tube according to claim 2, characterized in that, Step 3) The sterilization conditions include sterilization at a temperature of 121°C and a pressure of 0.1 MPa for 20 minutes.

5. A method for artificially raising the rubber tree bark beetle, characterized in that, Includes the following steps: A. Drilling holes in the surface of the rubber tree sawdust-cultured feed tube as described in any one of claims 2 to 5; B. Place the female adult rubber beetle in a feed tube and raise them until the offspring adults emerge.

6. The artificial breeding method according to claim 5, characterized in that, The diameter of the hole is 1~1.5mm and the depth is 5~8mm, with one hole drilled for each feed tube.

7. The artificial breeding method according to claim 5, characterized in that, After disinfection, the female adult insects are placed in a feed tube. The disinfection method includes: disinfecting with a 75% ethanol solution for 10 seconds, rinsing twice with sterile water for 10 seconds each time, and finally absorbing the moisture from the surface of the female adult insects with sterile filter paper.

8. The artificial breeding method according to claim 5, characterized in that, The conditions for raising the animals include a temperature of 25-27°C and a relative humidity of 45-55%.

9. The artificial breeding method according to claim 5, characterized in that, Once the rubber bark beetle has completely burrowed into the feed and excreted its frass, clean up the frass. Then, clean the frass excreted by the rubber bark beetle in the feed tube every 7 days until the adult offspring emerge.

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