Primer group and method for evaluating drug resistance level of murine population at high throughput

By designing universal primer combinations and next-generation sequencing technology, the problem of high-throughput drug resistance detection in various rodents has been solved, enabling rapid and economical drug resistance assessment, improving detection efficiency and accuracy, and reducing costs.

CN122303446APending Publication Date: 2026-06-30INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Patent Information

Application Number
CN202610683705.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-18
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing technologies are not suitable for rapid and economical high-throughput drug resistance testing in various rodent species, and traditional methods also present issues of animal ethics and high testing costs.

Method used

A set of universal primer combinations was designed to amplify the coding region of the Vkorc1 gene in various rodents. Combined with next-generation sequencing technology, high-throughput drug resistance assessment of various rodents was achieved. The primer combination includes three pairs of primers to amplify the three exons of the Vkorc1 gene, and next-generation sequencing technology is used for efficient assessment.

Benefits of technology

It significantly improves the species universality and throughput of rodent drug resistance detection, reduces detection costs, overcomes the limitation of Sanger sequencing in accurately analyzing heterozygotes with insertions and deletions, and enhances the ability to discover new resistance mutations.

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Abstract

The invention relates to a primer group and a method for evaluating the drug resistance level of a murine population at high throughput. The invention provides a group of universal degenerate primer combination for amplifying various mouse Vkorc1 genes. The primer combination comprises primers for amplifying a first exon, a second exon and a third exon of the Vkorc1 genes respectively. By designing a universal degenerate primer and coupling a next-generation sequencing technology, Vkorc1 gene coding regions of at least 15 kinds of cross-family rats can be amplified at the same time, the species universality and the detection flux for monitoring the drug resistance of the rats are remarkably improved, the large-scale screening cost is reduced, the defect that heterozygotes with insertion and deletion cannot be accurately analyzed through Sanger sequencing is overcome, and the method has the advantages of being high in specificity, high in specificity and high in sensitivity. The capability of discovering new resistance mutation is improved, and a standardized and low-cost technical scheme is provided for efficient evaluation of drug resistance of mice.
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