Vacuum ice-sculpture strawberry cell liquid skin care agent, its preparation method and application in skin moisturizing repair

Abstract

This invention discloses a vacuum-extracted strawberry cell sap skincare agent, its preparation method, and its application in skin moisturizing and repair, belonging to the field of skin care technology. The vacuum-extracted strawberry cell sap skincare agent comprises the following components in parts by weight: 70-85 parts strawberry cell sap, 0.1-0.5 parts sodium polyglutamate, 0.1-1 parts trehalose, 0.05-0.3 parts ceramide, 0.1-0.3 parts propylene glycol, 2-10 parts glycerin, 0.1-0.5 parts sodium hyaluronate, 0.2-0.8 parts hydroxyethyl cellulose, and 0.1-0.5 parts xanthan gum. This invention is the first to use strawberry cell sap as the core active ingredient in a skincare product, breaking through the limitations of traditional strawberry extract applications and fully exploring the skincare potential of natural molecules, water-soluble antioxidants, and fruit acids in the cell sap. Through the scientific formulation of sodium polyglutamate, trehalose, ceramide, and other ingredients, the moisturizing, repairing, and barrier-strengthening functions of the cell sap are significantly enhanced.

Description

Technical Field

[0001] This invention belongs to the field of skin care technology, specifically relating to a vacuum-extracted strawberry cell fluid skin care agent, its preparation method, and its application in skin moisturizing and repair. Background Technology

[0002] Currently, the cosmetics industry is increasingly focusing on the development and application of functional ingredients from natural sources, especially plant extracts, which have received widespread attention due to their high safety and comprehensive efficacy. Strawberry, a fruit rich in vitamin C, polyphenols, and fruit acids, has seen extensive reports on the application of its extracts in skincare products, widely used for moisturizing, whitening, anti-oxidation, and soothing. However, the application of strawberry extracts is already quite mature, its skincare activity and efficacy mechanisms are well-studied, and there are many similar products on the market, resulting in limited room for technological innovation.

[0003] On the other hand, while freeze-drying technology for strawberries is widely used in the food industry, the large amount of cell sap generated during this process is currently mainly discharged as wastewater and has not yet been developed for high-value utilization. Cell sap, released during the low-temperature sublimation process of strawberries, is rich in water-soluble volatiles, small-molecule organic acids, and some heat-resistant antioxidants. Although this type of cell sap has a natural source and certain potential skincare functions, there is currently no publicly available literature systematically discussing its application in the cosmetics field, and there is a lack of development and validation of related products. Therefore, this resource is underutilized.

[0004] Existing technologies have not disclosed methods for functional development of strawberry cell sap as a core active ingredient in skincare compositions, nor for enhancing its comprehensive effects such as moisturizing, repairing, brightening, and anti-oxidation through synergistic formulation with complementary ingredients. Addressing this technological gap, this invention aims to develop a skincare composition with significant skincare benefits, based on strawberry cell sap and combined with synergistic ingredients. The superior performance will be verified through controlled experiments, thereby achieving the high-value utilization and innovative application of strawberry cell sap. Summary of the Invention

[0005] This invention provides a strawberry cell fluid skin care agent containing sodium polyglutamate, trehalose, and ceramide, which can work synergistically with strawberry cell fluid to enhance skin hydration, rebuild the skin barrier, reduce transepidermal water loss, and significantly improve the product's long-lasting moisturizing and barrier repair capabilities.

[0006] The strawberry cell sap skin care agent comprises the following components in parts by weight: 70-85 parts strawberry cell sap, 0.1-0.5 parts sodium polyglutamate, 0.1-1 parts trehalose, 0.05-0.3 parts ceramide, 0.1-0.3 parts propylene glycol, 2-10 parts glycerol, 0.1-0.5 parts sodium hyaluronate, 0.2-0.8 parts hydroxyethyl cellulose, and 0.1-0.5 parts xanthan gum.

[0007] In one specific embodiment, the strawberry cell sap skincare agent comprises the following components in parts by weight: 75 parts strawberry cell sap, 0.3 parts sodium polyglutamate, 0.5 parts trehalose, 0.2 parts ceramide, 0.2 parts propylene glycol, 8 parts glycerol, 0.3 parts sodium hyaluronate, 0.5 parts hydroxyethyl cellulose, and 0.3 parts xanthan gum.

[0008] In one specific embodiment, the strawberry cell sap skincare agent comprises the following components in parts by weight: 85 parts strawberry cell sap, 0.5 parts sodium polyglutamate, 1 part trehalose, 0.3 parts ceramide, 0.3 parts propylene glycol, 10 parts glycerol, 0.4 parts sodium hyaluronate, 0.5 parts hydroxyethyl cellulose, and 0.5 parts xanthan gum.

[0009] This invention provides a method for preparing the above-mentioned strawberry cell sap skin care agent, the steps of which are as follows: Strawberry cell sap, sodium polyglutamate, glycerin, hydroxyethyl cellulose, and trehalose were mixed and stirred to obtain mixture A. After mixture A cooled, ceramide and propylene glycol were added, stirred, and kept warm to obtain mixture B. Sodium hyaluronate and xanthan gum were added to mixture B and stirred to obtain strawberry cell sap skin care agent.

[0010] In the above method for preparing strawberry cell sap skin care agent, the conditions for the first stirring are selected from: stirring at 100-300 r / min for 10-100 min at 80-100℃; the second stirring is for the purpose of uniform stirring; and the conditions for the third stirring are selected from: stirring at 100-300 r / min for 10-100 min at 20-40℃.

[0011] In the above method for preparing strawberry cell sap skin care agent, the static heat preservation conditions are selected from: static heat preservation at 30~60℃ for 10~60 minutes.

[0012] In the above method for preparing strawberry cell sap skin care agent, the cooling condition is selected from: cooling to 55~65℃.

[0013] This invention provides the application of the above-mentioned strawberry cell sap skin care agent in skin moisturizing, repair and barrier strengthening.

[0014] The beneficial effects of this invention are as follows: This invention is the first to use strawberry cell sap as the core active ingredient in skincare products, breaking through the limitations of traditional strawberry extract applications and fully exploring the skincare potential of natural molecules, water-soluble antioxidants, and fruit acids in the cell sap. Through the scientific formulation of ingredients such as sodium polyglutamate, trehalose, and ceramides, the moisturizing, repairing, and barrier-strengthening functions of the cell sap are significantly enhanced, creating a synergistic effect. Compared to existing technologies, this invention represents a significant technological advancement in raw material innovation, formula optimization, process gentleness, and efficacy verification. Controlled experiments have verified that its moisturizing, repairing, and antioxidant effects are superior to conventional strawberry extract products, offering advantages of high efficiency, safety, greenness, and low cost. This skincare agent excels in increasing skin hydration, repairing the skin barrier, promoting cell vitality, and scavenging free radicals. It is safe and gentle, suitable for various skin types, and therefore has excellent market application value and industrialization prospects in the field of skincare technology. Attached Figure Description

[0015] Figure 1 This is a picture of the actual product of the strawberry cell sap skin care product.

[0016] Figure 2 This is a performance test of strawberry cell sap.

[0017] Figure 3 This study aims to detect the microbial content and efficacy indicators in skin care products. Detailed Implementation

[0018] In this invention, a vacuum cold trap device is used to extract strawberry cell sap. The cell sap produced during the freeze-drying process of strawberries is captured and the gas is condensed by cooling. The "strawberry cell sap" is the cold trap water produced and collected during the freeze-drying process of strawberries, and the cell sap comes from Rizhao Changrong Biotechnology Co., Ltd.

[0019] Before preparation, the skin care agent of this invention requires the filtration and sterilization of each raw material. Specifically, the operation is as follows: Each raw material undergoes preliminary filtration, microfiltration (0.45μm or smaller filter membrane) to remove large particulate impurities and suspended solids; then deep sterilization / disinfection, ultraviolet UV sterilization or low-temperature pasteurization, to kill microorganisms in cell fluid and other materials; finally, precision filtration to further remove bacteria, viruses, and small molecule contaminants.

[0020] This invention addresses the problems of time-consuming extraction methods and significant nutrient loss in strawberry cell sap extraction by proposing a two-stage vacuum drying process: first, freezing to -35 to -20°C at a rate of 0.4-0.8°C / min, then heating to 20-25°C at a rate of 0.1-0.2°C / min, repeating this process three times. The resulting condensate is the strawberry cell sap.

[0021] Other materials used in this invention, unless otherwise stated, are commercially available. Other terms used in this invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art. The invention is further described in detail below with reference to specific embodiments and data. The following embodiments are merely illustrative and not intended to limit the scope of the invention in any way. Example 1

[0022] This embodiment provides a strawberry cell sap skin care agent, the formula of which is as follows: 75g strawberry cell sap, 0.3g sodium polyglutamate, 0.5g trehalose, 0.2g ceramide, 0.2g propylene glycol, 8g glycerin, 0.3g sodium hyaluronate, 0.5g hydroxyethyl cellulose, and 0.3g xanthan gum.

[0023] The preparation method of strawberry cell sap skin care agent is as follows: Strawberry cell sap, sodium polyglutamate, glycerin, hydroxyethyl cellulose, and trehalose were mixed and stirred at 200 rpm for 50 min at 90°C to obtain mixture A. When mixture A cooled to 65°C, ceramide and propylene glycol were added, and the mixture was stirred at 350 rpm until homogeneous. The mixture was then kept at 50°C for 40 min to obtain mixture B. Sodium hyaluronate and xanthan gum were added to mixture B, and the mixture was stirred at 200 rpm for 40 min at 30°C to obtain strawberry cell sap skin care agent.

[0024] The prepared strawberry cell sap skin care agent, such as Figure 1 As shown.

[0025] Samples were taken and tested for pH value, viscosity, and microbial indicators. After passing the tests, the samples were packaged to obtain the finished strawberry cell fluid skincare product. This skincare product is suitable for daily basic moisturizing and sensitive skin care. Example 2

[0026] This embodiment provides a strawberry cell sap skin care agent, the formula of which is as follows: 85g strawberry cell sap, 0.5g sodium polyglutamate, 1g trehalose, 0.3g ceramide, 0.3g propylene glycol, 10g glycerin, 0.4g sodium hyaluronate, 0.5g hydroxyethyl cellulose, and 0.5g xanthan gum.

[0027] The preparation method of strawberry cell sap skin care agent is as follows: Strawberry cell sap, sodium polyglutamate, glycerin, hydroxyethyl cellulose, and trehalose were mixed and stirred at 200 rpm for 50 min at 90°C to obtain mixture A. When mixture A cooled to 65°C, ceramide and propylene glycol were added, and the mixture was stirred at 350 rpm until homogeneous. The mixture was then kept at 50°C for 40 min to obtain mixture B. Sodium hyaluronate and xanthan gum were added to mixture B, and the mixture was stirred at 200 rpm for 40 min at 30°C to obtain strawberry cell sap skin care agent.

[0028] Samples were taken and tested for pH value, viscosity, and microbial indicators. After passing the tests, the samples were packaged to obtain the finished strawberry cell fluid skincare product. This skincare product has stronger moisturizing and barrier repair effects, making it suitable for use on dry and fragile skin requiring intensive repair.

[0029] Comparative Example 1 This comparative example provides a hydrating skincare agent based on plant extracts, with the following formula: 40g plant extracts, 12g hyaluronic acid, 20g sodium hyaluronate, 22g ceramide, 7g butylene glycol, 35g sodium lactate, 5g squalane, 1.2g grape seed oil, 2g coconut oil, 50g distilled water, 2.2g emulsifier (PEG-100 stearate), 3.2g humectant (glycerin), 3.6g thickener (xanthan gum), and 1.4g preservative (phenoxyethanol).

[0030] The above-mentioned plant extracts consist of 10 grams of purslane extract, 8 grams of aloe vera extract, 6 grams of chamomile extract, 6 grams of oat polypeptide, 6 grams of asiaticoside, and 4 grams of ginsenosides. All of the above extracts were purchased from Shaanxi Lvshengyuan Biological Products Manufacturing Co., Ltd.

[0031] The skincare formula described in this comparative example has a composition similar to common combinations in commercially available hydrating skincare products. Its ingredient selection emphasizes "hydration + barrier repair + natural plant active ingredients," aligning with the trend in the natural skincare market. Furthermore, all ingredients are widely used in commercial skincare products, making it a valuable reference standard.

[0032] The preparation method of the above-mentioned moisturizing skin care agent includes the following steps: Plant extracts and 30g of distilled water were mixed and stirred until homogeneous to obtain mixture A. Under stirring conditions, mixture A, hyaluronic acid, sodium hyaluronate, ceramide, and squalane were mixed, heated to 65°C, and kept at this temperature for 1 hour to obtain mixture B. Butylene glycol and sodium lactate were added to mixture B and stirred until homogeneous. This mixture was then placed in a fermenter at 55°C and fermented for 45 minutes to obtain mixture C. Mixture C was introduced into an extraction tower, 20g of distilled water was added, and extraction was carried out at 50°C for 30 minutes. The extract was collected, filtered to remove impurities, and mixture D was obtained. Grape seed oil and coconut oil were mixed, heated to 90°C, and kept at this temperature for 1 hour to obtain a mixed oil. Then, mixture D was heated to 80°C and kept at that temperature for 1.2 hours. An emulsifier was added, and the mixed oil was slowly and evenly added under rapid stirring to form an emulsion mixture. The emulsion mixture was poured into a reaction vessel, a humectant was added, and the mixture was heated to 70°C and stirred for 50 minutes. A thickener was then added, and the mixture was heated to 55°C and stirred for 40 minutes to obtain mixture E. Mixture E was introduced into a high-pressure homogenizer and homogenized at 1.9 MPa, 75°C, and 1100 rpm for 1.5 hours to obtain mixture F. Mixture F was filtered to remove impurities, and finally, a preservative was added and stirred thoroughly to obtain a moisturizing skin care agent.

[0033] Comparative Example 2 This comparative example provides a serum with hydrating, moisturizing, and repairing effects, and its formula is as follows: Seaweed extract 0.7g, ginseng extract 0.2g, tremella polysaccharide 0.4g, glycerin 5g, propylene glycol 8g, trehalose 0.5g, xanthan gum 0.1g, disodium EDTA 0.02g, tromethorphan 0.05g, water 90g.

[0034] The seaweed extract mentioned above was purchased from Qingdao Mingyue Seaweed Group, the ginseng extract was purchased from Jilin Aodong, and the tremella polysaccharide was purchased from Jiangxi Gannan Tremella Company.

[0035] This formula is similar to commercially available basic "hydrating and repairing serums," with a clear structure and no innovative ingredients. All ingredients are commonly used commercial raw materials, without novel active substances or sustained-release carriers. It represents a basic "hydrating + repairing" serum, suitable for use as a control group against experimental groups with innovative added ingredients. Its efficacy is standardized, with glycerin and propylene glycol forming traditional moisturizers; tremella polysaccharide and seaweed extract enhance the skin's moisture barrier function; EDTA, tromethamine, etc., maintain formula stability and pH buffering, resulting in a high degree of standardization. It is easily comparable, and due to its lightweight formula, differences can be clearly assessed. It can also serve as a basic control in cell experiments or skin barrier repair experiments.

[0036] The preparation method of the above-mentioned serum is as follows: Heat water to 80°C, then add propylene glycol and xanthan gum, stir to dissolve, and obtain mixture A; add trehalose, glycerin and disodium EDTA to mixture A, stir to dissolve at 80°C, and obtain mixture B; stir mixture B at 80°C at a stirring speed of 15 rpm for 30 minutes; then cool to 50°C, add tromethorphan, and stir evenly; continue to cool to 45°C, add seaweed extract, ginseng extract and tremella polysaccharide, and stir evenly; after stirring, defoam, and then cool to 38°C to obtain the essence.

[0037] Comparative Example 3 This comparative example provides a skin care agent with the following formulation: 75g distilled water, 0.3g sodium polyglutamate, 0.5g trehalose, 0.2g ceramide, 0.2g propylene glycol, 8g glycerin, 0.3g sodium hyaluronate, 0.5g hydroxyethyl cellulose, and 0.3g xanthan gum.

[0038] The preparation method for skin care products is as follows: Distilled water, sodium polyglutamate, glycerin, hydroxyethyl cellulose, and trehalose were mixed and stirred at 200 rpm for 50 min at 90°C to obtain mixture A. When mixture A cooled to 65°C, ceramide and propylene glycol were added, and the mixture was stirred at 350 rpm until homogeneous. The mixture was then kept at 50°C for 40 min to obtain mixture B. Sodium hyaluronate and xanthan gum were added to mixture B, and the mixture was stirred at 200 rpm for 40 min at 30°C to obtain the skin care agent.

[0039] I. Strawberry Cell Sap Performance Test The antioxidant activity, conductivity, pH value, and free radical scavenging ability of strawberry cell sap were tested, with strawberry juice, strawberry blanching water, and distilled water as comparisons.

[0040] Determination of total phenols and total flavonoids: Weigh 2.0 g of the test solution, add a small amount of pre-cooled 1% HCl-methanol solution, mix well under ice bath conditions, and transfer to a 20 mL graduated test tube. Rinse with 1% HCl-methanol solution, transfer the rinse solution to the test tube, dilute to the mark, mix well, and extract at 4℃ in the dark for 20 min, shaking several times during extraction. Then filter and collect the filtrate for later use. Using 1% HCl-methanol solution as a blank reference for zeroing, measure the absorbance of the filtrate at wavelengths of 280 nm and 325 nm, respectively, repeating three times. Calculate the total phenol content (OD280 / g) based on the absorbance value per gram of sample at wavelength 280 nm; the absorbance value at wavelength 325 nm represents the flavonoid content (OD325 / g), which represents the total flavonoids.

[0041] Conductivity Measurement: Conductivity is used to infer ion concentration by measuring the ratio of current to electric field strength in a solution. Conductivity is related to the properties, concentration, and temperature of the electrolyte. The measuring instrument is a conductivity meter with an error not exceeding 1%. Pure water and calibration solution are required. Measurement Procedure: Insert the electrodes into the solution to be tested and measure the resistivity between the two electrodes. Calibrate the cell constant using a standard solution with known conductivity.

[0042] pH determination: The pH is determined using acid-base titration. Acid-base titration is suitable for determining the pH of dilute solutions with high concentrations of hydrogen ions or hydroxide ions. This method is based on acid-base neutralization reactions. A known concentration of acid or base solution is added dropwise to the solution to be tested until the reaction is complete, at which point the pH of the solution will change. The titration endpoint is determined by observing the color change of the indicator, and the pH value of the solution can then be calculated.

[0043] DPPH assay: Dissolve DPPH reagent in DMSO to prepare a 20 mM solution. Dilute the 20 mM DPPH reagent 100 times with 95% ethanol and measure the absorbance at 515 nm. Adjust the absorbance of the DPPH reagent to 0.94~0.97. Add 20 μL of vitamin C to a 96-well plate, add 180 μL of the prepared DPPH reagent, react at room temperature in the dark for 10 minutes, and then measure the result at 515 nm.

[0044] Reducing power determination: Prepare sample solution gradients of different concentrations (50, 100, 250, 500, 1000 ppm), add 2.5 mL of phosphate buffer and 2.5 mL of potassium ferricyanide solution sequentially, mix well, place the system in a constant temperature water bath at 50℃ for 30 min, remove and add trichloroacetic acid solution, mix well, centrifuge at 3000 rpm for 10 min, take a certain amount of supernatant into another test tube, add deionized water and ferric chloride solution, mix well, and then measure the absorbance of the solution at 700 nm using a spectrophotometer.

[0045] The test results are as follows Figure 2 As shown: Antioxidant activity can be inferred from the total phenol and flavonoid content. Compared with strawberry juice and strawberry blanching water, the total phenol and flavonoid content in strawberry cell sap is lower, but compared with distilled water, its total phenol and flavonoid content is more considerable, indicating that strawberry cell sap has a certain antioxidant effect.

[0046] Electrical conductivity is related to the presence of water-soluble ionic active substances, minerals, or electrolytes. High conductivity indicates that the product contains a relatively high amount of water-soluble ionic active substances, minerals, or electrolytes. In hydrating, repairing, and soothing skincare products, the appropriate presence of ions helps maintain the skin's osmotic pressure balance and promotes the skin's absorption of moisture and nutrients. Compared to distilled water, strawberry cell sap has a slightly higher conductivity, which is beneficial for enhancing its hydrating and repairing abilities.

[0047] The skin's pH is 5.5-6.5, while the pH of strawberry cell sap is around 5.2. Therefore, strawberry cell sap is more in line with skincare requirements and standards.

[0048] Free radical scavenging capacity (DPPH scavenging rate) and reducing capacity showed that strawberry cell sap exhibited superior antioxidant capacity compared to distilled water.

[0049] II. Stability Testing of Skin Care Products 1. High-temperature treatment The product's stability and shelf life were evaluated by simulating high-temperature environments during storage and transportation. Temperature: 45±1°C; Time: 30 days; Environment: Constant temperature incubator, stored away from light.

[0050] The specific steps are as follows: The skincare product was dispensed into sealed containers (using light-proof / standard packaging bottles); three replicates were set up for each group. Observations were conducted on day 0 (control), day 7, day 15, and day 30.

[0051] 2. Low-temperature treatment Freeze-thaw cycle tests are used to assess the low-temperature stability of skincare products. These tests simulate the repeated freezing and thawing processes that occur during winter or cross-seasonal transportation to examine the stability of the emulsion system and active ingredients.

[0052] One freeze-thaw cycle: Freezing stage: -5 ± 2°C, 24 hours; Thawing stage: 25 ± 2°C, 24 hours; Total number of cycles: 3.

[0053] Similarly, the samples were divided into 3 sets of standard packaging containers; sealed and stored alternately in a freezer and a room temperature chamber. The samples were checked immediately after each cycle (3 cycles in total).

[0054] The experimental results are shown in Table 1: Table 1. Results of stability test of skin care products As shown in Table 1, the skin care agent of the present invention has good stability at both high and low temperatures.

[0055] III. Detection of Microbial Content and Efficacy Indicators in Skin Care Products The microbial content and efficacy indicators of Example 1, Example 2, Comparative Example 1, Comparative Example 2 and Comparative Example 3 were tested and labeled as Group A, Group B, Group C, Group D and Group E, respectively.

[0056] 1. Microbial content (CFU / g) Total bacterial count (TAMC) determination (refer to ISO 21149 / GB / T 13531.1) 10 samples were prepared -1 Up to 10 -6 Serial dilutions; take 1g of each dilution and inoculate it onto nutrient agar plates; incubate at 37℃ for 48±2 hours; record the number of plates with colonies and calculate CFU / g.

[0057] Total bacterial count = number of colonies × dilution factor / inoculum size.

[0058] Judgment criteria (China's "Cosmetic Safety Technical Specifications"): General cosmetics: ≤1000 CFU / g; Cosmetics for children, eyes, and broken skin: ≤500 CFU / g.

[0059] 2. Transepidermal water loss (TEWL) This experiment was a topical application test, using an open-cavity system (TM300) to measure transdermal water loss. The diffusion rate was calculated by measuring the water vapor concentration gradient at two points within the probe; the instrument was started and the zero point calibrated; the probe was placed perpendicularly to the skin surface (light touch, no pressure) and kept stable without movement; data was continuously read for 30-60 seconds; the average value after stabilization was recorded; measurements were taken 2-3 times at each site, and the average value was calculated. Data were measured before and after use (0h, 1h, 4h, 24h) to evaluate changes.

[0060] 3. DPPH free radical scavenging rate Dissolve DPPH reagent in DMSO to prepare a 20 mM solution. Dilute the 20 mM DPPH reagent 100 times with 95% ethanol and measure the absorbance at 515 nm. Adjust the absorbance of the DPPH reagent to 0.94 ~ 0.97. Add 20 μL of vitamin C to a 96-well plate, add 180 μL of the prepared DPPH reagent, and react for 10 minutes at room temperature in the dark. Measure the results at 515 nm.

[0061] 4. pH value The pH of the solution is determined using acid-base titration. Acid-base titration is suitable for determining the pH of dilute solutions with high concentrations of hydrogen ions or hydroxide ions. This method is based on acid-base neutralization reactions. A known concentration of acid or base solution is added dropwise to the solution to be tested until the reaction is complete, at which point the pH of the solution changes. The titration endpoint is determined by observing the color change of the indicator, and the pH value of the solution can then be calculated.

[0062] The test results are as follows Figure 3 As shown: Groups A and B had the lowest microbial content, close to 50 CFU / g and 60 CFU / g respectively, which was much lower than that of Groups C, D and E, demonstrating excellent hygiene and safety and meeting the microbial limit requirements for cosmetics (≤100 CFU / g). This indicates that the formula of the present invention has high cleanliness and strong anti-pollution ability.

[0063] The TEWL of groups A and B was significantly lower than that of groups C, D, and E, especially group A, which maintained a level close to 25 g / m³ over 24 hours. 2 The h value indicates that groups A and B have stronger skin barrier repair and water-locking abilities, effectively reducing moisture loss and maintaining healthy skin.

[0064] Groups A and B led in DPPH clearance rate, with Group A reaching nearly 35%, which was better than Groups C, D, and E. This demonstrates that Groups A and B have stronger antioxidant activity, can eliminate free radicals, and delay skin aging.

[0065] Regarding pH value, the pH values ​​of all five samples remained stable between 5.6 and 5.8, which is close to the slightly acidic pH of the skin and is suitable for sensitive skin.

[0066] IV. Skin Moisture Content Test The skin care products of Example 1, Example 2, Comparative Example 1, Comparative Example 2 and Comparative Example 3 were subjected to water retention tests.

[0067] Test subjects: A total of 100 subjects were tested, with 20 subjects recruited in each group. All subjects were female, with an average age of 35 years, and had neutral skin type, exhibiting a relatively uniform distribution. Skin moisture was measured using a moisture meter.

[0068] Test site selection: This formulation is a topical skin care product. The test area was selected as the mid-section of the inner forearm. The test area was approximately 3 cm × 3 cm, clean, dry, and free of wounds. The amount applied was 2g, spread evenly. The moisture content was measured using a moisture meter before application and 30 minutes later. The results were recorded as the average of the subjects' measurements and the standard deviation was also recorded.

[0069] The experimental results are shown in Table 2: Table 2 Results of Skin Moisture Content Test As shown in Table 2, the skin care agent of the present invention can significantly increase the moisture content in the skin and has excellent moisturizing effect.

[0070] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments. However, all simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the protection scope of the present invention.

Claims

1. A vacuum-extracted strawberry cell fluid skin care agent, characterized in that, The components comprise the following parts by mass: 70-85 parts strawberry cell sap, 0.1-0.5 parts sodium polyglutamate, 0.1-1 parts trehalose, 0.05-0.3 parts ceramide, 0.1-0.3 parts propylene glycol, 2-10 parts glycerol, 0.1-0.5 parts sodium hyaluronate, 0.2-0.8 parts hydroxyethyl cellulose, and 0.1-0.5 parts xanthan gum.

2. The strawberry cell sap skin care agent according to claim 1, characterized in that, The components comprise the following parts by mass: 75 parts strawberry cell sap, 0.3 parts sodium polyglutamate, 0.5 parts trehalose, 0.2 parts ceramide, 0.2 parts propylene glycol, 8 parts glycerol, 0.3 parts sodium hyaluronate, 0.5 parts hydroxyethyl cellulose, and 0.3 parts xanthan gum.

3. The strawberry cell sap skin care agent according to claim 1, characterized in that, The components comprise the following parts by mass: 85 parts strawberry cell sap, 0.5 parts sodium polyglutamate, 1 part trehalose, 0.3 parts ceramide, 0.3 parts propylene glycol, 10 parts glycerol, 0.4 parts sodium hyaluronate, 0.5 parts hydroxyethyl cellulose, and 0.5 parts xanthan gum.

4. The method for preparing the strawberry cell sap skin care agent according to any one of claims 1 to 3, characterized in that, The steps are as follows: Strawberry cell sap, sodium polyglutamate, glycerin, hydroxyethyl cellulose, and trehalose were mixed and stirred to obtain mixture A. After mixture A cooled, ceramide and propylene glycol were added, stirred, and kept warm to obtain mixture B. Sodium hyaluronate and xanthan gum were added to mixture B and stirred to obtain strawberry cell sap skin care agent.

5. The method for preparing the strawberry cell sap skin care agent according to claim 4, characterized in that, The conditions for the first stirring are selected from: stirring at 100-300 r / min for 10-100 min at 80-100℃; the second stirring is for the purpose of uniform mixing; the conditions for the third stirring are selected from: stirring at 100-300 r / min for 10-100 min at 20-40℃.

6. The method for preparing the strawberry cell sap skin care agent according to claim 4, characterized in that, The conditions for static heat preservation are selected from: static heat preservation at 30~60℃ for 10~60 minutes.

7. The method for preparing the strawberry cell sap skin care agent according to claim 6, characterized in that, The conditions for static heat preservation are selected from: static heat preservation at 50℃ for 40 minutes.

8. The method for preparing the strawberry cell sap skin care agent according to claim 4, characterized in that, The cooling conditions are selected from: cooling to 55~65℃.

9. The method for preparing the strawberry cell sap skin care agent according to claim 8, characterized in that, The cooling conditions are selected from: cooling to 65°C.

10. The use of the strawberry cell sap skin care agent according to any one of claims 1 to 3 in skin moisturizing, repairing or barrier strengthening.

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