An immune system humanized mouse model targeting knockout of plk2 and a construction method and application thereof
By knocking out the PLK2 gene using the CRISPR/Cas9 system in human CD34+ hematopoietic stem cells, a humanized mouse model of the immune system with PLK2 knockout was constructed, solving the problem of low T lymphocyte reconstruction efficiency, achieving a significant increase in the proportion of human T cells, and providing a more efficient research tool.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
- Filing Date
- 2026-04-29
- Publication Date
- 2026-07-17
AI Technical Summary
In existing humanized mouse models, T lymphocyte reconstitution efficiency is low and function is incomplete, which limits their application in T cell-dependent immunity research. Existing gene modification methods have limited effectiveness or lack specificity.
By knocking out the PLK2 gene in human CD34+ hematopoietic stem cells using the CRISPR/Cas9 gene editing system, a humanized mouse model of the immune system with targeted knockout of PLK2 was constructed, increasing the proportion of human CD3-positive T cells.
It significantly improved the reconstructed proportion of human T cells in humanized mouse models, provided a research tool that is closer to the composition and function of the human immune system, overcame the problem of low T cell reconstructed efficiency, and provided a more advantageous research platform.
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Figure CN122397685A_ABST