Skin conditioning agents and methods for their preparation, repair skin care

Abstract

This application provides a skin conditioning agent and its preparation method, as well as a repairing skin care product. The skin conditioning agent comprises, by weight percentage: 64-74% Bifida Ferment Lysate; 15-25% Butylene Glycol; 5-14% Herbal Extracts; 0.5-1.5% Solubilizer; and the remainder is water. The herbal extracts include at least one of Gardenia jasminoides extract, Lonicera japonica extract, Chamomile extract, Gentiana scabra root extract, Forsythia suspensa extract, Cinnamomum camphora bark extract, Paeonia suffruticosa root bark extract, and Glycyrrhiza uralensis extract. This application uses Bifida Ferment Lysate as the main ingredient to repair the stratum corneum of the skin. It is compounded with multiple herbal extracts to form a multi-layered system of action that provides immediate soothing, anti-inflammatory, and deep repair, effectively improving skin sensitivity issues such as redness, burning, dryness, itching, and stinging, and strengthening the skin barrier and its own tolerance.

Description

Technical Field

[0001] This application relates to the field of skincare technology, specifically to a skin conditioning agent and its preparation method, and a repair skincare product. Background Technology

[0002] Currently, most repair skincare products on the market containing yeast and plant extracts use physical compounding methods. The large molecular active ingredients in these products are difficult for the skin to absorb, resulting in low bioavailability. Furthermore, the product systems have poor compatibility, and they are prone to separation and clouding during long-term storage. Many products also rely on chemical preservatives, which are highly irritating and unsuitable for sensitive skin. In addition, conventional skincare products offer only one function, making it difficult to simultaneously achieve long-lasting barrier repair, anti-inflammatory soothing, and moisturizing stabilization, resulting in unsatisfactory effects. Summary of the Invention

[0003] This application aims to overcome the shortcomings of existing skin care products, such as low activity utilization, poor stability, and strong irritation, and to provide a skin conditioning agent with excellent efficacy and its preparation method. Based on this, a repair skin care product can be made, which can effectively repair the skin barrier, soothe sensitivity and discomfort, and the product is gentle and stable.

[0004] To achieve the above objectives, this application provides the following technical solution:

[0005] As a first aspect, this application relates to a skin conditioning agent comprising, by weight percentage:

[0006] Bifida ferment filtrate: 64-74%;

[0007] Butylene glycol 15-25%;

[0008] Herbal extracts 5-14%;

[0009] Solubilizer 0.5-1.5%;

[0010] The rest is water;

[0011] The herbal extracts include at least one of the following: gardenia extract, honeysuckle flower extract, golden chamomile extract, gentian root extract, forsythia extract, wampee bark extract, peony root bark extract, and licorice root extract.

[0012] Further details: The content of each component of the herbal extract, by mass percentage, is as follows: Gardenia extract 1-3%, Honeysuckle flower extract 0.5-1.5%, Golden Chamomile extract 0.5-1.5%, Gentian root extract 0.5-1.5%, Forsythia extract 0.5-1.5%, Wampee bark extract 0.5-1.5%, Paeonia root bark extract 0.5-1.5%, and Glycyrrhiza inflata extract 0.3-0.7%.

[0013] Further, by weight percentage, the herbal extract also includes 0.1-0.5% frankincense extract and 0.1-0.5% myrrh extract.

[0014] Further configuration: The solubilizer comprises PEG-40 hydrogenated castor oil.

[0015] Further configuration: It also includes a preservative comprising 0.3-0.7% by weight of 1,2-hexanediol and 0.3-0.7% by weight of p-hydroxyacetophenone.

[0016] As a second aspect, this application relates to a method for preparing the above-mentioned skin conditioning agent, characterized by comprising the following steps:

[0017] S1. Mix the herbal extract, microbial culture medium and deionized water evenly, put them into a fermenter and sterilize them at high temperature to obtain a sterile fermentation substrate.

[0018] S2. Inoculate the sterilized fermentation substrate with Bifida ferment lysate, and ferment in a closed system to complete the biological fermentation reaction and obtain a fermentation mixture.

[0019] S3. Centrifuge the fermentation mixture obtained in S2 and collect the clarified Bifida ferment filtrate.

[0020] S4. Add butanediol and solubilizer to the obtained Bifida ferment filtrate in sequence, stir and mix evenly, then add preservative and stir until the system is completely homogeneous.

[0021] S5. Filter and package the compounded liquid to obtain the finished skin conditioning agent.

[0022] Further configuration: The microbial culture medium includes MRS culture medium.

[0023] Further settings: In S2, the fermentation culture temperature is 28-35℃, the fermentation time is 48-72 hours, and the pH range of the fermentation system is 4.0-6.

[0024] Further setting: The pH range of the skin conditioning agent is 4.5-5.5.

[0025] As a third aspect, this application relates to a repairing skin care product, comprising the following ingredients by weight percentage: 8-15% skin conditioning agent, 5-10% glycerin, 2.0-5.0% butylene glycol, 0.5-1.5% hexanediol, 1.0-3.0% betaine, 0.3-0.8% phenazine, 0.05-0.2% macromolecular hyaluronic acid, 0.1-0.3% carbomer 941, 0.2-0.5% cetearyl alcohol polyether-80, with the remainder being water; wherein the skin conditioning agent is the skin conditioning agent as described in any one of claims 1-5.

[0026] Compared with existing technologies, the solution in this application has the following advantages:

[0027] 1. In the skin conditioning agent involved in this application, bifida ferment filtrate is used as the main ingredient. The polypeptides, amino acids and small molecule polysaccharides produced by its metabolism can repair the stratum corneum of the skin. It is combined with a variety of herbal extracts to form a multi-layered system of action that can soothe, reduce inflammation and sensitivity, and deeply repair the skin. It can effectively improve sensitive skin problems such as redness, burning, dryness, itching and stinging, and strengthen the skin barrier and its own tolerance.

[0028] 2. In the preparation method for preparing skin conditioning agents involved in this application, various herbal extracts are directly used as fermentation substrates to participate in the entire co-fermentation process of Bifida Ferment Lysate. The herbal macromolecular active substances are biodegraded, transformed and activated by microbial metabolism, and the poorly absorbed macromolecular components are transformed into small molecule active substances, effectively improving the water solubility and transdermal ability of the active ingredients.

[0029] 3. In the preparation method of the skin conditioning agent involved in this application, high-temperature sterilization can eliminate interference from miscellaneous bacteria and ensure the purity and activity of the fermentation product; the operation mode of stepwise feeding at room temperature and two-stage filtration can not only maximize the preservation of the efficacy of active substances, but also ensure that the liquid is clear and uniform.

[0030] 4. The skin repair products involved in this application use a co-fermented skin conditioning agent as the core, combined with polyols, hyaluronic acid and other excipients to form a complete skin care system, which can quickly replenish moisture, soothe redness and dryness, repair damaged skin barrier and has excellent comprehensive repair effect.

[0031] Additional aspects and advantages of this application will be set forth in part in the description which follows, and will become apparent from the description or may be learned by practice of this application. Attached Figure Description

[0032] The above and / or additional aspects and advantages of this application will become apparent and readily understood from the following description of the embodiments taken in conjunction with the accompanying drawings, wherein:

[0033] Figure 1 This is a process flow diagram of the preparation method for the skin conditioning agent used in this application. Detailed Implementation

[0034] The embodiments of this application are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this application, and should not be construed as limiting this application.

[0035] It should be understood that the various steps described in the method embodiments of the present invention may be performed in different orders and / or in parallel. Furthermore, the method embodiments may include additional steps and / or omit the steps shown. The scope of the present invention is not limited in this respect.

[0036] The term "comprising" and its variations as used herein are open-ended, meaning "including but not limited to". The term "connection" can refer to a direct connection or an indirect connection via intermediate components (elements). The term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one additional embodiment"; the term "some embodiments" means "at least some embodiments". Definitions of other terms will be given in the following description.

[0037] It should be noted that the concepts of "first" and "second" mentioned in this invention are only used to distinguish between devices, modules or units, and are not intended to limit these devices, modules or units to necessarily be different devices, modules or units, nor are they intended to limit the order or interdependence of the functions performed by these devices, modules or units.

[0038] This application discloses a skin conditioning agent, the raw materials of which, by mass percentage, include: 64-74% Bifida Ferment Lysate, 15-25% Butylene Glycol, 5-14% Herbal Extracts, 0.5-1.5% Solubilizer, 0.6-1.4% Preservative, and the remainder being deionized water. This application uses Bifida Ferment Lysate as the core active ingredient, combined with a variety of natural herbal extracts, and synergistically enhances the effects with solubilizers, preservatives and other adjuvants. The proportions of each component are scientifically reasonable, the active ingredients are highly stable, and it has moisturizing, soothing and skin barrier repair effects.

[0039] Specifically, Bifida Ferment Lysate, as the core active ingredient of this application, is rich in active substances such as peptides, amino acids, organic acids, and small-molecule polysaccharides. It can repair the damaged skin barrier, enhance the skin's ability to resist external stimuli, and soothe sensitive conditions such as redness and dryness, maintaining the skin's healthy state for a long time, and has good repairing and stabilizing effects. Butylene glycol, used as a water-soluble solvent, can improve the solubility and dispersion uniformity of various active substances in Bifida Ferment Lysate and herbal extracts, reducing the precipitation and stratification of effective ingredients, thereby ensuring the uniformity and stability of the skin conditioning agent system of this application. Furthermore, the molecule of butylene glycol contains hydrophilic groups, which can effectively moisturize the stratum corneum of the skin, improve the dryness and roughness of the skin, and enhance the basic moisturizing ability of the skin conditioning agent.

[0040] The herbal extracts in this application include at least one of the following: gardenia extract, honeysuckle flower extract, golden chamomile extract, gentian root extract, forsythia extract, wampee bark extract, peony root bark extract, and licorice root extract. Among these, gardenia extract, golden chamomile extract, and peony root bark extract primarily function to quickly soothe the skin, effectively improving redness, burning, and stinging caused by external stimuli, providing excellent immediate relief. Honeysuckle flower extract, forsythia extract, and wampee bark extract primarily inhibit superficial skin inflammation, relieving dryness and itching, thus reducing skin inflammation. Gentian root extract and licorice root extract focus on strengthening the skin barrier, enhancing the skin's own tolerance, and fundamentally improving fragile and sensitive skin, achieving long-term skin stabilization. The herbal extracts in this application work synergistically to improve skin sensitivity at multiple targets, forming a multi-layered system of action that provides immediate soothing, anti-inflammatory and anti-allergic effects, and barrier repair, with complementary and synergistic efficacy.

[0041] Furthermore, the herbal extract in this application also includes frankincense extract and myrrh extract. Frankincense extract contains active ingredients such as boswellic acid, which can repair damaged skin tissue and further soothe dryness and tightness. Myrrh extract has both soothing, anti-inflammatory, and repairing effects, which can alleviate recurring skin sensitivity issues. Combined with frankincense extract, they synergistically enhance the repair effect on damaged skin. This application, by additionally compounding frankincense extract and myrrh extract into the original compound herbal extract system, further deepens the skin repair effect and strengthens the overall soothing and stabilizing ability, comprehensively improving problems such as damaged skin barrier and fragile skin, on the basis of the original immediate redness reduction, anti-inflammatory and antipruritic, and barrier stabilizing effects.

[0042] The herbal extracts in this embodiment, by mass percentage, are: Gardenia extract 1-3%, Honeysuckle flower extract 0.5-1.5%, Golden Chamomile extract 0.5-1.5%, Gentian root extract 0.5-1.5%, Forsythia extract 0.5-1.5%, Wampee bark extract 0.5-1.5%, Paeonia root bark extract 0.5-1.5%, Glycyrrhiza inflata extract 0.3-0.7%, Frankincense extract 0.1-0.5%, and Myrrh extract 0.1-0.5%. The herbal extracts are compounded in a gradient dosage, achieving immediate soothing, multi-functional maintenance, and deep repair. The balanced proportions of each component create a synergistic effect, ensuring skincare efficacy while effectively avoiding problems such as discoloration, layering, odor, and irritation of the solution, thus balancing product safety, stability, and sensory quality.

[0043] Furthermore, this application also includes phospholipid-modified plant polyphenols. Phospholipid-modified plant polyphenols can enhance the water solubility, permeability, and system compatibility of polyphenolic active ingredients, thereby enabling the phospholipid-modified plant polyphenols to first complex with the herbal extracts. The phospholipid groups can improve the physicochemical properties of various herbal extracts, reduce the mutual influence between different plant components, and prevent problems such as layering, precipitation, and discoloration after mixing. Moreover, the complexation can form a skin-like lipid structure, making it easier for the active ingredients in the herbal extracts to penetrate the stratum corneum of the skin, improving the utilization rate of the active ingredients, and thus enhancing the penetration and absorption of the herbal extracts. Simultaneously, phospholipid-modified plant polyphenols can also protect the herbal components, delaying their oxidative inactivation, further improving the overall stability and efficacy of the formulation.

[0044] In this embodiment, the phospholipid-modified plant polyphenols are made from soybean phospholipids. Soybean phospholipids have excellent biocompatibility and can be used as a carrier to achieve efficient complexation with herbal extracts, improve the solubility and penetration of active ingredients, and help repair the skin barrier. The overall formulation is stable and has high safety.

[0045] The solubilizer used in this application is PEG-40 hydrogenated castor oil. PEG-40 hydrogenated castor oil is a nonionic surfactant with excellent emulsifying and solubilizing abilities. It can efficiently dissolve fat-soluble active ingredients in the system and avoid stratification and turbidity.

[0046] The addition of preservatives in this application can inhibit microbial growth, prevent product spoilage, and extend the shelf life. The preservatives in this embodiment, by weight percentage, include 0.3-0.7% 1,2-hexanediol and 0.3-0.7% p-hydroxyacetophenone. 1,2-Hexanediol has both preservative and moisturizing effects, inhibiting the growth of various microorganisms while enhancing the formula's moisturizing ability and optimizing skin feel. p-Hydroxyacetophenone has a broad antibacterial spectrum and also possesses antioxidant properties, protecting herbal active ingredients from oxidative damage. The combination of these two ingredients synergistically enhances the antibacterial effect, effectively preventing the skin conditioning agent of this application from spoiling.

[0047] In summary, the skin conditioning agent of this application uses a high-content Bifida ferment filtrate as the main repairing matrix, combined with butylene glycol to build a stable moisturizing base. It also features a gradient blend of various herbal extracts, supplemented with frankincense and myrrh extracts to deepen the repairing effect. Combined with a highly efficient solubilizing system of PEG-40 hydrogenated castor oil and a gentle preservative system of 1,2-hexanediol and p-hydroxyacetophenone, the overall formula is homogeneous, stable, gentle, and low-irritant. Through the scientific proportioning and synergistic layering of its components, this application forms a complete skincare chain that provides immediate soothing and redness reduction, anti-inflammatory and anti-allergic effects, deep barrier repair, and long-lasting skin stabilization and anti-allergy benefits. It effectively improves skin redness, stinging, dryness, itching, fragility, and sensitivity, while also ensuring excellent moisturizing and repairing effects, safety, and storage stability, making it suitable for long-term conditioning use on sensitive and barrier-damaged skin.

[0048] This application also discloses a method for preparing the above-mentioned skin conditioning agent. Please refer to... Figure 1 Specifically, it includes the following steps:

[0049] S1. Thoroughly mix the herbal extracts, microbial culture medium, and deionized water, and put them into a fermenter for high-temperature sterilization to completely kill off bacteria and microorganisms, obtain sterile fermentation substrate, and ensure that the subsequent fermentation process is not contaminated by bacteria.

[0050] First, gardenia extract, honeysuckle flower extract, golden chamomile extract, gentian root extract, forsythia extract, wampee bark extract, peony root bark extract, licorice root extract, frankincense extract, and myrrh extract are taken according to the specified ratio. The various herbal extracts are then mixed and stirred evenly to prepare a compound herbal mixture.

[0051] The microbial culture medium used is MRS medium, which can provide sufficient carbon source, nitrogen source and trace elements for the growth, reproduction and metabolic transformation of Bifida ferment lysate, ensuring the activity of the strain and fermentation efficiency, while being suitable for the co-fermentation system environment of herbal components.

[0052] S2. After the sterilized fermentation substrate is cooled to a suitable inoculation temperature, Bifida ferment lysate is introduced into the fermenter and fermented in a sealed, constant-temperature environment. Through biological fermentation, active substances such as polypeptides, amino acids, small molecule polysaccharides, and organic acids are generated, completing the fermentation reaction and obtaining a fermentation mixture.

[0053] The fermentation temperature was controlled at 28-35℃, the fermentation time was controlled at 48-72h, and the pH of the fermentation system was maintained at 4.0-6.0 throughout the process to ensure the normal proliferation and metabolic efficiency of Bifida ferment lysate.

[0054] S3. The fermentation mixture obtained in step S2 is subjected to high-speed centrifugation to remove cell residue, unreacted substrate and impurities. The clear liquid in the upper layer is collected to obtain a high-purity, stable Bifida ferment filtrate.

[0055] This application employs a co-fermentation process combining herbal extracts and Bifida ferment lysate, differing from traditional formulations that simply physically combine herbal extracts with fermentation filtrate. In this application, all herbal extracts are used as fermentation substrates beforehand, sterilized together with MRS medium and deionized water, and participate in the entire Bifida ferment lysate fermentation process. During microbial metabolism, Bifida ferment lysate can biotransform, degrade, and activate large molecular active substances in the herbs, converting poorly absorbed macromolecular components in the herbal extracts into smaller molecular active substances, significantly improving the solubility, permeability, and bioactivity of the herbal extracts. The Bifida ferment filtrate obtained after centrifugation and filtration after fermentation is itself enriched with both Bifida ferment lysate metabolites and biotransformed herbal active components.

[0056] Under S4 and temperature conditions, butanediol and solubilizer were added sequentially to the filtrate of the obtained Bifida ferment lysate, and the mixture was stirred continuously until all components were completely dispersed and mixed evenly to improve the solubility and stability of the system. Then, preservatives were added and the mixture was stirred until the whole system was completely homogeneous and clear to achieve synergistic compatibility of the functional components.

[0057] S5. After the mixture is evenly mixed, it is precisely filtered to remove trace amounts of insoluble impurities and particles from the system, ensuring that the mixture is clear and transparent and the system is stable. Then, it is packaged using aseptic technology to finally obtain a skin conditioning agent with uniform components, stable properties, mild and safe properties, and moisturizing, soothing and barrier repair effects.

[0058] When filtering the compounded filtrate, a two-stage filtration process can be performed. First, a 100-mesh filter is used for coarse filtration to remove large particulate impurities. Then, a 0.45μm polypropylene filter element is used for fine filtration to further remove fine suspended solids and ensure that the filtrate is clear and transparent.

[0059] The preparation method of this application employs a co-fermentation process of herbs and Bifida ferment lysate. Herbal extracts are used as fermentation substrates and co-fermented with Bifida ferment lysate. Through microbial metabolism, the macromolecular components of the herbs undergo biotransformation, enhancing the solubility and transdermal absorption of active ingredients, achieving a deep synergistic effect between yeast metabolites and herbal active substances. The entire process involves high-temperature sterilization before inoculation and fermentation, effectively preventing contamination by other microorganisms and ensuring the purity and activity of the fermentation products. Subsequent step-by-step addition of ingredients at room temperature maximizes the preservation of the efficacy of various active substances. A two-stage filtration process removes impurities step by step, ensuring a clear, uniform, and stable liquid. The entire process is simple, controllable, and suitable for industrial production. The resulting skin conditioning agent possesses excellent moisturizing, soothing, anti-inflammatory, and barrier-repairing effects, with significantly improved product stability, safety, and user experience.

[0060] In addition, in other embodiments, a herbal pre-complexation step can be added between the preparation of the fermentation substrate. This involves first mixing all the herbal extracts in the specified proportions with phospholipid-based plant polyphenols carried by soybean lecithin to complete the pre-complexation treatment. Then, the complexed herbal compound is added to a fermenter, mixed with MRS medium and deionized water, and subsequently prepared according to the complete process of high-temperature sterilization, inoculation fermentation, centrifugation, compounding, filtration, and packaging. This embodiment utilizes the amphiphilic molecular structure of soybean lecithin to encapsulate the herbal active ingredients in advance. This avoids the thermal oxidation and degradation of polyphenols and flavonoids in the herbs during subsequent high-temperature sterilization, maximizing the preservation of the herbal skincare activity. Furthermore, it improves the dispersibility of hydrophobic herbal components in the aqueous fermentation system, preventing sedimentation and aggregation of herbal raw materials, allowing for more thorough contact between the Bifida ferment lysate strain and the fermentation substrate, and enhancing the biotransformation efficiency of co-fermentation. Meanwhile, the phospholipid structure can be retained in the final fermentation filtrate, which helps the active ingredients penetrate the skin's stratum corneum, further enhancing the product's repair and soothing effects. Moreover, this pre-complexation process does not inhibit the normal growth and metabolism of Bifida Ferment Lysate and has good compatibility with the overall co-fermentation process.

[0061] The skin conditioning agent in this application has a pH value controlled between 4.5 and 5.5, which matches the slightly acidic environment of human skin. This helps maintain the homeostasis of the stratum corneum, reduces external irritation, and ensures that various plant extracts and bifida ferment lysate active ingredients can exert their effects stably, further enhancing the product's soothing and repairing effects.

[0062] The technical solution of this application will be described below with reference to specific embodiments. The following embodiments are only used to explain this application and do not limit the scope of protection of this application.

[0063] Example 1

[0064] The skin conditioning agent of this embodiment comprises the following ingredients by weight percentage: 69% Bifida ferment filtrate, 20% butylene glycol, 2% gardenia extract, 1% honeysuckle flower extract, 1% golden chamomile extract, 1% gentian root extract, 1% forsythia extract, 1% wampee bark extract, 1% peony root bark extract, 0.5% licorice root extract, 0.3% frankincense extract, 0.2% myrrh extract, 1% PEG-40 hydrogenated castor oil, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, and the balance being deionized water.

[0065] The preparation method of the above-mentioned skin conditioning agent includes the following steps: all herbal extracts, MRS culture medium and deionized water are mixed evenly and put into a fermenter for high-temperature sterilization to obtain a sterile fermentation substrate; after the fermentation substrate is cooled to 30~35℃, Bifida ferment lysate is inoculated and fermented in a sealed constant temperature for 48h to obtain a fermentation mixture; the fermentation mixture is centrifuged at high speed and the clarified Bifida ferment lysate filtrate is collected; at room temperature, butylene glycol and PEG-40 hydrogenated castor oil are added to the filtrate in sequence, stirred and mixed, and then 1,2-hexanediol and p-hydroxyacetophenone are added and stirred until the system is homogeneous; finally, the liquid is coarsely filtered through a 100-mesh filter and finely filtered through a 0.45μm polypropylene filter cartridge, and aseptically packaged to obtain the finished skin conditioning agent.

[0066] Example 2

[0067] The skin conditioning agent of this embodiment comprises the following ingredients by weight percentage: 74% Bifida ferment filtrate, 15% butylene glycol, 1% gardenia extract, 0.5% honeysuckle flower extract, 0.5% golden chamomile extract, 0.5% gentian root extract, 0.5% forsythia extract, 0.5% wampee bark extract, 0.5% peony root bark extract, 0.3% licorice root extract, 0.1% frankincense extract, 0.1% myrrh extract, 1.5% PEG-40 hydrogenated castor oil, 0.7% 1,2-hexanediol, 0.7% p-hydroxyacetophenone, and the balance being deionized water.

[0068] The process flow, process parameters and operating steps of the above-mentioned skin conditioning agent preparation method are the same as those in Example 1.

[0069] Example 3

[0070] The skin conditioning agent of this embodiment comprises the following ingredients by weight percentage: 70% Bifida ferment filtrate, 20% butylene glycol, 2% gardenia extract, 1% honeysuckle flower extract, 1% golden chamomile extract, 1% gentian root extract, 1% forsythia extract, 1% wampee bark extract, 1% peony root bark extract, 0.5% licorice root extract, 0.3% frankincense extract, 0.2% myrrh extract, 1% PEG-40 hydrogenated castor oil, and the balance being deionized water.

[0071] The preparation method of the above-mentioned skin conditioning agent includes the following steps: all herbal extracts, MRS culture medium and deionized water are mixed evenly and put into a fermenter for high-temperature sterilization to obtain a sterile fermentation substrate; after the fermentation substrate is cooled to 30~35℃, Bifida ferment lysate is inoculated and fermented in a sealed constant temperature for 48h to obtain a fermentation mixture; the fermentation mixture is centrifuged at high speed and the clarified Bifida ferment lysate filtrate is collected; butylene glycol and PEG-40 hydrogenated castor oil are added to the filtrate at room temperature in sequence and stirred until the system is homogeneous; finally, the liquid is coarsely filtered through a 100-mesh filter and finely filtered through a 0.45μm polypropylene filter cartridge, and aseptically packaged to obtain the finished skin conditioning agent.

[0072] Example 4

[0073] The skin conditioning agent of this embodiment comprises the following ingredients by weight percentage: 69% Bifida ferment filtrate, 20% butylene glycol, 2% gardenia extract, 1% honeysuckle flower extract, 1% golden chamomile extract, 1% gentian root extract, 1% forsythia extract, 1% wampee bark extract, 1% peony root bark extract, 0.5% licorice root extract, 0.3% frankincense extract, 0.2% myrrh extract, 0.6% soybean lecithin, 1% PEG-40 hydrogenated castor oil, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, and the balance being deionized water.

[0074] The preparation method of the above-mentioned skin conditioning agent is as follows: First, the herbal extract and soybean lecithin are mixed and stirred to complete the pre-complexation. Then, the pre-complexed mixture, MRS culture medium and deionized water are mixed evenly and put into a fermenter for high-temperature sterilization to obtain a sterile fermentation substrate. After the fermentation substrate is cooled to 30~35℃, Bifida ferment lysate is inoculated and fermented in a sealed constant temperature for 48h to obtain a fermentation mixture. The fermentation mixture is centrifuged at high speed, and the clarified Bifida ferment lysate filtrate is collected. Butylene glycol and PEG-40 hydrogenated castor oil are added to the filtrate in sequence at room temperature and stirred evenly. Then, 1,2-hexanediol, p-hydroxyacetophenone and other preservatives are added and stirred until the system is homogeneous. Finally, the liquid is coarsely filtered through a 100-mesh filter and finely filtered through a 0.45μm polypropylene filter cartridge, and aseptically packaged to obtain the finished skin conditioning agent.

[0075] Example 5

[0076] The skin conditioning agent of this embodiment comprises the following ingredients by weight percentage: 69% Bifida ferment filtrate, 20% butylene glycol, 2% gardenia extract, 1% honeysuckle flower extract, 1% golden chamomile extract, 1% gentian root extract, 1% forsythia extract, 1% wampee bark extract, 1% peony root bark extract, 0.5% licorice root extract, 0.3% frankincense extract, 0.2% myrrh extract, 1% PEG-40 hydrogenated castor oil, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, and the balance being deionized water.

[0077] The preparation method of the above-mentioned skin conditioning agent includes the following steps: MRS culture medium and deionized water are mixed evenly and placed in a fermenter for high-temperature sterilization to obtain a sterile fermentation substrate; after cooling to 30-35℃, Bifida Ferment Lysate is inoculated and fermented in a sealed, constant-temperature environment for 48 hours to obtain a fermentation mixture; the fermentation mixture is centrifuged at high speed, and the clear liquid at the top is collected to obtain the Bifida Ferment Lysate fermentation filtrate. At room temperature, butylene glycol and PEG-40 hydrogenated castor oil are added sequentially to the Bifida Ferment Lysate fermentation filtrate, and stirred until completely mixed. Then, the total amount of herbal extracts is added, and stirring continues until the materials are evenly dispersed; then 1,2-hexanediol and p-hydroxyacetophenone are added, and stirring is continued until the entire system is homogeneous. The solution is then sequentially coarsely filtered through a 100-mesh filter and finely filtered through a 0.45μm polypropylene filter cartridge, and aseptically packaged to obtain the finished product.

[0078] It should be noted that during the fermentation stage, MRS medium was used to prepare the Bifida ferment filtrate, and no herbal extracts were added throughout the process.

[0079] To verify the efficacy of the skin conditioning agent of this application, animal experiments were conducted on mouse skin barrier repair and soothing anti-inflammatory effects.

[0080] In vitro soothing and anti-inflammatory validation experiment

[0081] This application selected SPF-grade healthy mice and randomly divided them into 8 groups of 10 mice each, with a balanced sex ratio. Specifically, these included Example 1, Example 2, Example 3, Example 4, Example 5, Control Group, and Blank Group. In Example 1, Example 2, Example 3, Example 4, Example 5, and Control Group, 5% sodium dodecyl sulfate (SDS) solution was applied to the hair-removed area on the back of the mice for 5 consecutive days to establish a mouse model of skin barrier damage, redness, dryness, and inflammation. The mice in the Blank Group did not receive any SDS stimulation treatment to establish the model, and their back skin remained intact and healthy throughout the process. Each day, an equal amount of physiological saline was applied to the same hair-removed area. All other breeding environment and daily management conditions were completely consistent with the other groups to eliminate the interference of irrelevant variables in the breeding environment on the experimental results.

[0082] After modeling, each group applied the corresponding test sample evenly to the damaged skin on the back of the mice every morning and evening, with a single application volume of 20 μL, for 7 consecutive days. The appearance of the mouse back skin was observed daily. On the 7th day of the experiment, the skin moisture content and transepidermal water loss (TEWL) of the mouse back skin were measured, and the content of the skin inflammatory factor IL-6 was also measured.

[0083] The experimental results are shown in the table below:

[0084] Group Skin moisture content (%) Transepidermal water loss (TEWL) (g / (m²·h)) Inflammatory factor IL-6 (pg / mL) Skin appearance Example 1 54.16 12.43 35.27 The redness has mostly subsided, with only minor peeling remaining. Example 2 Group 52.79 13.15 39.14 The redness has mostly subsided, with only minor peeling remaining. Example 3 Group 53.81 12.68 37.05 The redness has mostly subsided, with only minor peeling remaining. Example 4 58.93 9.72 27.41 The redness has mostly subsided, with only minor peeling remaining. Example 5 group 45.34 18.26 52.39 The skin has recovered well, with no redness or peeling, and is close to normal skin. control group 31.22 26.71 78.62 Noticeable redness, dryness, peeling, and slight swelling. Blank group 50.20 10.12 21.35 Smooth skin, without redness or peeling

[0085] Compared to the control group, all sample groups showed increased skin hydration, reduced transepidermal water loss, and decreased levels of inflammatory factors, demonstrating moisturizing, barrier repair, and soothing / anti-inflammatory effects. The moisturizing and repairing effects are derived from Bifida Ferment Lysate and butylene glycol in the formula, which replenish skin moisture, fill gaps in the stratum corneum, and reduce water loss. The soothing / anti-inflammatory effects come from a blend of ten herbal extracts. Honeysuckle, chamomile, and licorice root can inhibit the release of inflammatory factors; gardenia, forsythia, phellodendron, and peony bark can soothe redness and stinging; and frankincense and myrrh synergistically repair damaged skin. Simultaneously, the small-molecule peptides and amino acids produced by Bifida Ferment Lysate fermentation further nourish the skin. These multiple components work synergistically to achieve moisturizing, barrier repair, and soothing / anti-inflammatory effects.

[0086] In addition, Examples 1 to 4, which use herbal co-fermentation technology, have significantly better overall efficacy than Example 5, which uses traditional physical compounding. This proves that the participation of herbs in the co-fermentation of Bifida Ferment Lysate can effectively activate herbal active ingredients and enhance skincare efficacy.

[0087] Example 4, which adds a soybean lecithin pre-complexing process, has the best efficacy, indicating that soybean lecithin pre-complexing can protect the active ingredients of herbs, enhance the transdermal absorption capacity of active substances, and further strengthen the product's barrier repair and soothing anti-inflammatory effects.

[0088] In summary, the prepared skin conditioning agent is gentle and non-irritating, and can effectively repair damaged skin barriers and relieve skin redness, dryness, and inflammation.

[0089] Corrosion protection system effectiveness verification experiment

[0090] Common bacterial strains used in cosmetic contamination experiments included Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger. Mixed bacterial solutions were quantitatively inoculated into each group of test samples to achieve an initial bacterial concentration of 1 × 10⁻⁶. 5 The samples were sealed and incubated at a constant temperature of 25℃. The number of residual viable bacteria in the samples was tested at 7, 14 and 28 days after inoculation. The preservative qualification standard was determined according to the "Cosmetic Safety Technical Specifications": total bacterial count ≤100 CFU / g, total mold and yeast count ≤100 CFU / g.

[0091] Please refer to the table below for the experimental results:

[0092] Group 7-day viable count (CFU / g) 14-day viable count (CFU / g) 28-day viable count (CFU / g) Example 1 120 21 0 Example 2 Group 110 18 0 Example 3 Group 136 49 0 Example 4 92 15 0 Example 5 group 316 153 24 Control group (physiological saline) 9520 16430 27860

[0093] The blank control group used physiological saline as a blank control, without any preservative or antibacterial components. After inoculation with microorganisms, the number of colonies continued to increase significantly, indicating that it had no antibacterial or preservative ability and could be used as an effective control benchmark. Compared with the blank control group, all examples and comparative products could quickly kill the mixed bacteria, meet the national standard for cosmetic preservation, and effectively inhibit the growth of bacteria and mold at room temperature, ensuring the safety of the product's shelf life.

[0094] Compared to the traditional physical compounding method in Example 5, Examples 1 to 4, prepared using the herbal co-fermentation process, exhibited faster antibacterial action and higher viable bacteria removal efficiency. This is because the organic acids produced by Bifida ferment lysate fermentation can synergistically inhibit bacterial growth with the compounded preservatives, enhancing the system's preservative capacity. Example 4 showed the best antibacterial effect. The soybean lecithin pre-complexing system stabilized the liquid system, preventing the decomposition of active ingredients from affecting the stability of the preservative system and further enhancing its long-lasting preservative capacity. Example 3, without the addition of 1,2-hexanediol and p-hydroxyacetophenone compounded chemical preservatives, still met cosmetic preservative standards. This is because Bifida ferment lysate generates small-molecule organic acids such as lactic acid and acetic acid during anaerobic fermentation, lowering the system's pH and creating an acidic environment unfavorable to bacterial and fungal growth, achieving endogenous natural preservative protection. Simultaneously, the synergistic combination of Bifida ferment lysate, butylene glycol, and herbal extracts eliminates the need for external chemical preservatives to achieve satisfactory preservative effects, further improving product gentleness and reducing the risk of skin irritation.

[0095] This application also relates to a repairing skin care product, which comprises the following ingredients by weight percentage: 8-15% skin conditioning agent, 5-10% glycerin, 2.0%-5.0% butylene glycol, 0.5-1.5% hexanediol, 1.0-3.0% betaine, 0.3-0.8% succinate, 0.05-0.2% macromolecular hyaluronic acid, 0.1-0.3% carbomer 941, 0.2-0.5% cetearyl alcohol polyether-80, with the balance being deionized water; wherein the skin conditioning agent is the skin conditioning agent described above.

[0096] This repair skincare product uses skin conditioning agents as its main ingredient. Bifida ferment lysate fermentation produces small-molecule peptides and amino acids to repair the stratum corneum. It is also formulated with ten herbal extracts to synergistically reduce inflammation and redness, effectively lowering the level of inflammatory factors in the skin. Glycerin, butylene glycol, betaine, and other polyol moisturizing components quickly replenish skin moisture, lock in moisture in the stratum corneum, reduce transepidermal water loss, and improve dry, flaky skin. The added high-molecular-weight hyaluronic acid forms a breathable, moisturizing protective film on the skin surface, blocking external irritants and helping to strengthen the skin's physical barrier. Carbomer 941 and cetearyl alcohol polyether-80 are thickeners and dispersants that stabilize the overall liquid system of the skincare product, preventing active ingredients from separating and stratifying, and improving the product's application feel. Hexanediol and succinate form a synergistic preservative system with the endogenous fermented organic acids of the skin conditioning agents, ensuring product stability throughout its shelf life without the need for irritating preservatives.

[0097] The above description is only a partial embodiment of this application. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of this application, and these improvements and modifications should also be considered within the scope of protection of this application.

Claims

1. A skin conditioning agent, characterized in that, Including raw materials by weight percentage: Bifida ferment filtrate: 64-74%; Butylene glycol 15-25%; Herbal extracts 5-14%; Solubilizer 0.5-1.5%; The rest is water; The herbal extracts include at least one of the following: gardenia extract, honeysuckle flower extract, golden chamomile extract, gentian root extract, forsythia extract, wampee bark extract, peony root bark extract, and licorice root extract.

2. The skin conditioning agent according to claim 1, characterized in that, The components of the herbal extract, by mass percentage, are as follows: Gardenia extract 1-3%, Honeysuckle flower extract 0.5-1.5%, Golden Chamomile extract 0.5-1.5%, Gentian root extract 0.5-1.5%, Forsythia extract 0.5-1.5%, Wampee bark extract 0.5-1.5%, Paeonia root bark extract 0.5-1.5%, and Glycyrrhiza inflata extract 0.3-0.7%.

3. The skin conditioning agent according to claim 2, characterized in that, The herbal extract also includes 0.1-0.5% frankincense extract and 0.1-0.5% myrrh extract by weight percentage.

4. The skin conditioning agent according to claim 1, characterized in that, The solubilizer includes PEG-40 hydrogenated castor oil.

5. The skin conditioning agent according to claim 1, characterized in that, It also includes preservatives, which comprise 0.3-0.7% by weight of 1,2-hexanediol and 0.3-0.7% by weight of p-hydroxyacetophenone.

6. A method for preparing the skin conditioning agent according to any one of claims 1-5, characterized in that, Includes the following steps: S1. Mix the herbal extract, microbial culture medium and deionized water evenly, put them into a fermenter and sterilize them at high temperature to obtain a sterile fermentation substrate. S2. Inoculate the sterilized fermentation substrate with Bifida ferment lysate, and ferment in a closed system to complete the biological fermentation reaction and obtain a fermentation mixture. S3. Centrifuge the fermentation mixture obtained in S2 and collect the clarified Bifida ferment filtrate. S4. Add butanediol and solubilizer to the obtained Bifida ferment filtrate in sequence, stir and mix evenly, then add preservative and stir until the system is completely homogeneous. S5. Filter and package the compounded liquid to obtain the finished skin conditioning agent.

7. The preparation method according to claim 6, characterized in that, The microbial culture medium includes MRS medium.

8. The preparation method according to claim 6, characterized in that, In S2, the fermentation temperature is 28-35℃, the fermentation time is 48-72 hours, and the pH range of the fermentation system is 4.0-6.

9. The preparation method according to claim 6, characterized in that, The pH range of the skin conditioning agent is 4.5-5.

5.

10. A repairing skincare product, characterized in that, The ingredients, by weight percentage, are: skin conditioning agent 8-15%, glycerin 5-10%, butylene glycol 2.0-5.0%, hexanediol 0.5-1.5%, betaine 1.0-3.0%, succinate 0.3-0.8%, macromolecular hyaluronic acid 0.05-0.2%, carbomer 941 0.1-0.3%, cetearyl alcohol polyether-80 0.2-0.5%, with the remainder being water; The skin conditioning agent is the skin conditioning agent according to any one of claims 1-5.

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