A method for quantitatively detecting HBV preS2 protein and identifying serum subtypes
By combining liquid chromatography-tandem mass spectrometry (LC-MS/MS) with nucleic acid sequencing, accurate quantification of preS2 protein and precise identification of HBV serum subtypes were achieved. This method solves the problem of insufficient sensitivity of traditional HBsAg detection methods in occult hepatitis B infection and is suitable for high-throughput detection of large numbers of clinical samples.
CN122410046APending Publication Date: 2026-07-17SHANDONG PROVINCIAL HOSPITAL AFFILIATED TO SHANDONG FIRST MEDICAL UNIVERSITY (SHANDONG PROVINCIAL HOSPITAL)
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANDONG PROVINCIAL HOSPITAL AFFILIATED TO SHANDONG FIRST MEDICAL UNIVERSITY (SHANDONG PROVINCIAL HOSPITAL)
- Filing Date
- 2026-04-23
- Publication Date
- 2026-07-17
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Figure CN122410046A_ABST
Abstract
本发明属于生物医学检测技术领域，具体涉及一种定量检测HBV preS2蛋白并鉴定血清亚型的方法。包括：采用LC‑MS / MS采集各亚型preS2蛋白特异性离子对信号；以标准曲线结合内标校正计算蛋白含量；同时提取血清HBV DNA，对preS2区进行PCR扩增和测序，与已知亚型参考序列比对确认亚型归属。本发明检测下限达100 pg / mL，可同时区分adw、adr、adr‑m、ayw、ayr五种亚型。在HBsAg阴性的隐匿性乙型肝炎患者中，该方法的preS2蛋白检出率达60%；在功能性治愈患者中，preS2蛋白检出率达70%。本发明通过质谱定量与核酸测序双重验证，为HBV特殊人群提供了可靠检测手段。
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