Liquid phase process for the preparation of oligonucleotides
By using nucleoprotective base groups (NPGs) to protect building blocks in liquid-phase oligonucleotide synthesis, the concentration polarization problem in LPOS technology is solved, achieving efficient membrane separation and purification, suitable for industrial-scale oligonucleotide preparation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- EXACTMER LTD
- Filing Date
- 2024-06-14
- Publication Date
- 2026-07-17
AI Technical Summary
Existing membrane-assisted liquid-phase oligonucleotide synthesis (LPOS) technology is prone to concentration polarization at high concentrations, leading to increased viscosity and aggregation, resulting in inefficient membrane separation and fouling problems.
Using building blocks protected by nucleoprotective groups (NPGs), grown oligonucleotides are separated by membrane filtration, reducing the effects of concentration polarization. Building blocks protected by nucleoprotective groups (NPGs) reduce viscosity and aggregation during membrane filtration, and the frequency and position of the group Q are adjusted to accommodate changes in oligonucleotide length.
This technology enables efficient membrane separation at high concentrations, reduces pollution, and allows for the direct preparation of oligonucleotides with increased length on an industrial scale, thereby improving synthesis efficiency and purity.
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