Biosensor

Abstract

The present application relates to a biosensor comprising: a substrate; a first gate formed on the substrate; a second gate formed on the substrate; and a potential measuring section formed between the first gate and the second gate, measuring the potential of the first gate and the potential of the second gate, a channel section being formed between the first gate and the potential measuring section and between the potential measuring section and the second gate, so that the gate potential is kept constant, thereby having the effect of reducing measurement noise and improving sensing accuracy.

Description

Technical Field

[0001] This invention relates to biosensors, and more specifically, to biosensors that generate electrical signals by detecting substances in a living organism. Background Technology

[0002] In recent years, with the development of science and technology and increased attention to quality of life, the importance of disease diagnosis and prevention, food, and the environment in people's lives has been growing. As a result, the necessity of measuring the concentration of organic or inorganic substances in samples for specific processes in the fields of food chemistry and industrial chemistry, or for analyzing pollutants in the environmental field, has gradually increased, and much effort has been put into this endeavor.

[0003] A biosensor is a device that integrates biological substances such as enzymes, microorganisms, antibodies, receptors, and DNA probes into an electrochemical or physicochemical element (transducer). It senses signals and measures concentrations of electrode-active substances or physical changes generated by the reaction with the analyte through electrochemical, optical, thermal, or piezoelectric methods.

[0004] In general, the detection of biological substances using biosensors involves a combination of complex processes such as filtering, metering, mixing, transporting, reacting, and washing. Therefore, historically, the detection of biological substances was performed manually in laboratory units utilizing various facilities.

[0005] Therefore, with the development of biosensor technology, the simultaneous development of fluid processing technologies to automate and standardize the diagnostic process has become very important in order to achieve low-cost and high-efficiency diagnosis.

[0006] Equipment used for biological substance detection requires cleaning the flow path before measuring each biological sample. To diagnose multiple samples, large volumes of buffer solution need to be stored in tanks, posing a risk of spoilage and necessitating the periodic emptying of waste solution after diagnosis. When using such equipment, diagnostic speed and accuracy decrease when diagnosing large numbers of samples, thus limiting its effectiveness.

[0007] To address this issue, disposable biosensor cartridges with built-in flow paths for buffer and sample solutions can be used. These cartridges are used only once, eliminating the need for separate cleaning processes. Furthermore, each cartridge is provided with a disposable buffer solution, eliminating the possibility of buffer solution deterioration. Finally, the waste solution can be emptied when the cartridge is discarded.

[0008] Relatedly, a field diagnosis molecular diagnostic system is disclosed in Korean Patent Publication KR10-2022-0047600A.

[0009] The aforementioned on-site diagnostic molecular diagnostic system includes a box and diagnostic instruments. Biological samples are stored in the box and inserted into the diagnostic instruments for various sample processing steps.

[0010] The aforementioned on-site diagnostic molecular diagnostic system is configured such that the box is inserted into the diagnostic instrument while standing upright in the vertical direction.

[0011] Furthermore, a pneumatic device is provided to allow the buffer solution or sample solution to flow. When the box is inserted, pneumatic power is supplied to the inside of the box to allow the buffer solution or sample solution to flow.

[0012] However, as mentioned above, when the box is inserted in a direction perpendicular to the ground, the buffer solution or sample solution may flow due to gravity. Therefore, the flow of the buffer solution or sample solution is independent of the operation of the diagnostic instrument and cannot be precisely controlled.

[0013] In addition, the relatively large pneumatic devices need to be installed in the diagnostic instruments, which can lead to an increase in the size of the diagnostic equipment, causing inconvenience when transporting it to the site where the diagnosis is needed or when setting it up on-site.

[0014] In addition, pneumatic devices are difficult to control in fine detail, making it difficult to control the flow rate and velocity of the buffer solution or sample solution flowing inside the box. This can lead to errors in diagnostic data, resulting in decreased accuracy and limitations.

[0015] In the case of conventional biosensors, as the state of the fluid changes, the gate potential changes, resulting in errors and a decrease in sensing accuracy. Summary of the Invention

[0016] Technical issues

[0017] The present invention was proposed to improve the problems mentioned above. The object of the present invention is to provide a biosensor that can reduce measurement noise and improve sensing accuracy by keeping the gate potential constant.

[0018] In addition, the present invention aims to provide a biosensor that increases the degree of freedom of fluid movement direction, thereby improving sensing accuracy.

[0019] means of solving technical problems

[0020] To achieve the objectives described above, the biosensor of the present invention includes: a substrate; a first gate formed on the substrate; a second gate formed on the substrate; and a potential measuring portion formed between the first gate and the second gate, for measuring the potential of the first gate and the potential of the second gate, wherein a channel portion is formed between the first gate and the potential measuring portion and between the potential measuring portion and the second gate.

[0021] The channel portion may include: a first drain-source channel formed between the first gate and the potential measuring portion; a second drain-source channel formed between the potential measuring portion and the second gate; a third drain-source channel formed between the first gate and the potential measuring portion, and formed at a position spaced apart from the first drain-source channel; and a fourth drain-source channel formed between the potential measuring portion and the second gate, and formed at a position spaced apart from the second drain-source channel.

[0022] The first gate, the potential measuring unit, and the second gate may be formed on the same straight line.

[0023] It is possible that a first gap is formed between the first gate and the potential measuring unit, and a second gap is formed between the potential measuring unit and the second gate, wherein the first gap and the second gap are the same.

[0024] The biosensor may further include: a first drain electrode formed on one side of the first drain-source channel; a first source electrode formed on the other side of the first drain-source channel; a second drain electrode formed on one side of the second drain-source channel; a second source electrode formed on the other side of the second drain-source channel; a third drain electrode formed on one side of the third drain-source channel; a third source electrode formed on the other side of the third drain-source channel; a fourth drain electrode formed on one side of the fourth drain-source channel; and a fourth source electrode formed on the other side of the fourth drain-source channel.

[0025] The biosensor may further include: a first gate electrode connected to the first gate; a second gate electrode connected to the second gate; and a potential measuring electrode connected to the potential measuring unit. The first gate electrode and the second gate electrode are electrically connected to one end of the potential compensation unit, which compensates the potential of the first gate and the potential of the second gate to a preset potential. The potential measuring electrode is electrically connected to the other end of the potential compensation unit.

[0026] To achieve the objectives described above, the biosensor of the present invention may include: a substrate; a gate formed on the substrate; and a potential measuring portion formed on the substrate at a spaced interval to measure the potential of the gate, wherein a channel portion is formed between the gate and the potential measuring portion.

[0027] The channel portion may include: a first drain-source channel formed between the gate and the potential measurement portion; and a second drain-source channel formed between the gate and the potential measurement portion, and formed at a position spaced apart from the first drain-source channel.

[0028] It is possible that the gate and the potential measuring section are formed on the same straight line.

[0029] To achieve the objectives described above, the sensing system of the present invention may include: a biosensor housing having a flow path for a solution; a biosensor disposed in the biosensor housing; and a diagnostic device for analyzing signals received from the biosensor, wherein the biosensor includes: a substrate; a first gate formed on the substrate; a second gate formed on the substrate; and a potential measuring portion formed between the first gate and the second gate, for measuring the potential of the first gate and the potential of the second gate, and a channel portion formed between the first gate and the potential measuring portion and between the potential measuring portion and the second gate.

[0030] The channel portion may include: a first drain-source channel formed between the first gate and the potential measuring portion; a second drain-source channel formed between the potential measuring portion and the second gate; a third drain-source channel formed between the first gate and the potential measuring portion, and formed at a position spaced apart from the first drain-source channel; and a fourth drain-source channel formed between the potential measuring portion and the second gate, and formed at a position spaced apart from the second drain-source channel.

[0031] The first gate, the potential measuring unit, and the second gate may be formed in a straight line in the same direction as the flow direction of the solution.

[0032] It is possible that a first gap is formed between the first gate and the potential measuring unit, and a second gap is formed between the potential measuring unit and the second gate, wherein the first gap and the second gap are the same.

[0033] The diagnostic device may include a potential compensation unit that compensates for the potential of the first gate and the second gate to maintain a preset potential.

[0034] The biosensor may further include: a first gate electrode connected to the first gate; a second gate electrode connected to the second gate; and a potential measuring electrode connected to the potential measuring unit. The first gate electrode and the second gate electrode are electrically connected to one end of the potential compensation unit, and the potential measuring electrode is electrically connected to the other end of the potential compensation unit.

[0035] Invention Effects

[0036] A linear electric field is applied to the channel from both sides of the first and second gates, thus generating a stable field effect. Furthermore, this structure of the first gate, the potential measurement section, and the second gate increases the degree of freedom in the fluid movement direction, thereby improving sensing accuracy.

[0037] By positioning the first gate at the lower part of the inflow port formed on the bottom frame of the biosensor box and the second gate at the lower part of the outlet port formed on the bottom frame of the biosensor box, the potential can be kept constant, thereby reducing measurement noise and improving sensing accuracy.

[0038] The potential compensation section ensures that the potentials applied to the first and second gates of the channel remain constant, thereby reducing measurement noise and improving sensing accuracy. Attached Figure Description

[0039] Figure 1 This is a diagram illustrating a biosensor box and diagnostic device according to an embodiment of the present invention.

[0040] Figures 2 to 4 This is a diagram illustrating a biosensor box according to an embodiment of the present invention.

[0041] Figure 5 It is used for in Figure 4 A top view illustrating the state of the upper shell removed.

[0042] Figure 6 This is an exploded perspective view used to illustrate the framework of one embodiment of the present invention.

[0043] Figure 7 This is a top view used to illustrate the upper frame of one embodiment of the present invention.

[0044] Figure 8 This is a cross-sectional view of the frame according to an embodiment of the present invention.

[0045] Figure 9 This is a bottom view used to illustrate a channel formed on the upper frame in one embodiment of the present invention.

[0046] Figure 10 This is a diagram illustrating the combination of the frame, biosensor, and printed circuit board in a biosensor box according to an embodiment of the present invention.

[0047] Figure 11 This is a diagram illustrating the sensor junction in a biosensor box according to an embodiment of the present invention.

[0048] Figure 12 This is a diagram illustrating the state in which a biosensor is incorporated into a biosensor box according to an embodiment of the present invention.

[0049] Figure 13 This is a cross-sectional view illustrating the contact between the biosensor and the printed circuit board in a biosensor box according to an embodiment of the present invention.

[0050] Figure 14 and Figure 15 This is a cross-sectional view used to illustrate the configuration of the biosensor and printed circuit board in a biosensor box according to an embodiment of the present invention, as well as the configuration of the flow path flowing on the biosensor.

[0051] Figure 16 This is a diagram illustrating the process of diagnosing a sample solution placed into a biosensor cartridge using a diagnostic apparatus according to an embodiment of the present invention.

[0052] Figure 17 This is a cross-sectional view illustrating a solution flow biosensor according to an embodiment of the present invention.

[0053] Figures 18 to 20 This is a diagram illustrating the circuit configuration of a biosensor according to an embodiment of the present invention.

[0054] Figure 21 and Figure 22 This is a graph used to illustrate the noise reduction of the transfer curve in one embodiment of the present invention.

[0055] Figure 23 and Figure 24This is a diagram used to illustrate the potential compensation situation of one embodiment of the present invention. Detailed Implementation

[0056] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0057] This invention can have various modifications and embodiments, with specific embodiments illustrated in the accompanying drawings and detailed descriptions provided. This invention is not limited to specific embodiments, but includes all modifications, equivalents, and substitutions falling within the scope of the invention's concept and technology.

[0058] In describing this invention, terms such as "first" and "second" are used to describe various constituent elements, but these terms do not limit the constituent elements. These terms are only used to distinguish one constituent element from others. For example, without departing from the scope of this invention, a first constituent element may be named a second constituent element, and similarly, a second constituent element may be named a first constituent element.

[0059] The term “and / or” can include a combination of multiple related items or any one of multiple related recorded items.

[0060] When a constituent element is mentioned as being "connected" or "linked" to other constituent elements, it can be understood either as being directly connected or linked to other constituent elements, or as having other constituent elements in between. Conversely, when a constituent element is mentioned as being "directly connected" or "directly linked" to other constituent elements, it can be understood as having no other constituent elements in between.

[0061] The terminology used in this application is for illustrative purposes only and is not intended to limit the scope of the invention. Unless otherwise specified herein, singular expressions include plural meanings.

[0062] In this application, terms such as "comprising" or "having" refer to the presence of features, figures, steps, actions, constituent elements, components, or combinations thereof as described in the specification, and do not preclude the existence or possibility of one or more other features or figures, steps, actions, constituent elements, components, or combinations thereof.

[0063] Unless otherwise defined herein, all terms used herein, including technical or scientific terms, shall have the same meaning as understood by a person skilled in the art in the ordinary sense. Predefined terms used in the ordinary sense shall be understood to have the same meaning as understood in the relevant art text, and shall not be construed as having an ideal or overly formal meaning unless explicitly defined in this application.

[0064] Furthermore, the following embodiments are provided to illustrate the invention more fully to those skilled in the art. For clarity, the shapes and sizes of the elements in the accompanying drawings may be exaggerated.

[0065] Figure 1 This is a diagram illustrating a biosensor box and diagnostic device according to an embodiment of the present invention. Figures 2 to 4 This is a diagram illustrating a biosensor box according to an embodiment of the present invention. Figure 5 It is used for in Figure 4 A top view illustrating the state of the upper shell removed.

[0066] Reference Figures 1 to 5 In one embodiment of the present invention, a biosensor cartridge 1 can be integrated into a diagnostic device 2 to sense biological substances, thereby enabling the diagnosis of diseases, etc. As one embodiment, the biosensor cartridge 1 is inserted into the diagnostic device 2 in a horizontally oriented configuration. The biosensor cartridge 1 allows buffer solutions and sample solutions to flow internally, and causes the biosensor 400 to undergo an electrochemical reaction with the biological substances. The resulting electrical changes are transmitted to the diagnostic device 2 via a printed circuit board 500.

[0067] A biosensor box 1 according to one embodiment of the present invention includes a housing 100, a frame 200, a can 300, a biosensor 400, a printed circuit board 500, a channel 600, a tube 700, a valve 800, and a pump 900.

[0068] A frame 200 is housed inside the outer casing 100. A canister 300 is formed on the upper side of the frame 200, and a channel 600 can be formed inside the frame 200. A tube 700, a valve section 800, and a pump section 900 are integrated into the frame 200, and a biosensor 400 and a printed circuit board 500 are detachably integrated into it.

[0069] In this invention, with frame 200 as a reference, the direction in which the buffer solution is immersed is called the upper side, and with frame 200 as a reference, the opposite direction of the upper side is called the lower side. The direction in which the biosensor box 1 is inserted into the diagnostic device 2 can be called the front. That is, the direction in which the printed circuit board 500 is disposed relative to the housing 100 can be called the front. In addition, the opposite direction of the front can be called the rear. Furthermore, when viewing the front from the rear end of the housing 100, the direction disposed on the left can be called the left side, and the direction disposed on the right can be called the right side.

[0070] The outer shell 100 forms the shape of the biosensor box 1, and can house the frame 200, the can 300, the biosensor 400, the printed circuit board 500, the channel 600, the tube 700, the valve 800 and the pump 900 inside.

[0071] The housing 100 may include an upper housing 110 and a lower housing 120.

[0072] As one embodiment, the upper outer shell 110 is formed into a four-cornered box shape with the lower side open, and a buffer solution inlet hole 111, a sample solution inlet hole 112, a valve connection hole 113 and a pump connection hole 114 can be formed on the upper surface.

[0073] The buffer solution inlet 111 can be formed to allow the buffer solution to flow into it. The buffer solution inlet 111 can also be formed in the blister pack 111a. The blister pack 111a can be disposed on the upper vertical part of the buffer solution tank 310. The buffer solution inlet 111 can be disposed on the upper vertical side of the buffer solution tank 310, and the buffer solution inlet 111 can be formed to communicate with the internal space of the buffer solution tank 310.

[0074] The blister pack 3 can be integrated into the blister pack storage groove 111a. The blister pack storage groove 111a is formed by a downward recess on the upper surface of the upper outer shell 110, corresponding to the shape of the blister pack 3. As one embodiment, the blister pack storage groove 111a is formed in the shape of a square groove or a circular groove on the upper surface of the upper outer shell 110. With this structure, when the blister pack 3 is broken open while stored inside the blister pack storage groove 111a, the buffer solution can flow into the buffer solution tank 310 through the buffer solution inlet hole 111.

[0075] The pump unit 900 is rotatably coupled to the frame 200, and as it rotates, it applies pressure to the tube 700, thereby generating flow force on the buffer solution or sample solution flowing in the channel 600.

[0076] The pump unit 900 may include a pump knob 910 and a bearing 920.

[0077] The pump knob 910 can be disposed on the upper surface of the upper frame 220. The pump knob 910 can be rotatably attached to the pipe housing 222. The pump knob 910 can be rotatably housed in the pump housing slot 222a.

[0078] Specifically, the pump knob 910 includes a shaft 911, a support portion 912, and a drive groove 913.

[0079] At least one or more vent holes 111b and 116 may be formed on the upper surface of the upper outer casing 110. The vent holes 111b and 116 are formed to allow air inside the casing 100 to be discharged to the outside. A first vent hole 111b may be formed on the upper surface of the upper outer casing 110. The first vent hole 111b may be disposed in the vertical upper part of the buffer solution tank 310. The first vent hole 111b may be configured to communicate with the internal space of the buffer solution tank 310. When the buffer solution flows into the buffer solution tank 310, air inside the buffer solution tank 310 can be discharged to the outside through the first vent hole 111b. This prevents the internal pressure of the buffer solution tank 310 from rising and reduces the probability of air being mixed into the buffer solution.

[0080] A first air passage 111c may also be formed on the upper surface of the upper housing 110. The first air passage 111c is formed to communicate with the first vent 111b and can be formed in the shape of a groove along the upper surface of the upper housing 110. As an embodiment, the air passage 111c can be formed as a straight groove or as a shape that bends multiple times on the upper surface of the upper housing 110. This is to ensure the maximum flow path length within a limited area. When a label 130 is adhered to the upper surface of the upper housing 110, the first air passage 111c forms a space between the upper housing 110 and the label 130 and can collect the air discharged through the first vent 111b.

[0081] The sample solution inlet hole 112 is configured to allow sample solution to flow into it. As one embodiment, the sample solution inlet hole 112 may be disposed on the vertical upper side of the sample solution container 320. The sample solution inlet hole 112 may be configured to communicate with the internal space of the sample solution container 320. As one embodiment, a stopper 112a is detachably attached to the sample solution inlet hole 112. With the stopper 112a attached, the sample solution inlet hole 112 can be blocked. Furthermore, when the stopper 112a is removed, the sample solution inlet hole 112 is opened, allowing sample solution to be added. After the sample solution is added, blocking the sample solution inlet hole 112 with the stopper 112a prevents foreign matter from flowing into the sample solution inlet hole 112.

[0082] The valve connection hole 113 may be formed as part of the valve portion 800, extending through the valve connection hole 113. The valve connection hole 113 may be disposed on the upper side of the valve joint portion 223. The valve connection hole 113 is formed as a circular hole, and the valve knob 820 may be disposed through the valve connection hole 113. The valve knob 820 may rotate within the valve connection hole 113.

[0083] At least a portion of the valve knob 820 is rotatably housed inside the valve housing 810.

[0084] A knob groove 823 can be formed in the valve knob 820 to allow external force to be applied. When the biosensor box 1 is combined with the diagnostic device 2, the knob groove 823 can be combined with a valve actuator (not shown) provided in the diagnostic device 2.

[0085] The pump connection hole 114 can be formed as part of the pump section 900, extending through the pump connection hole 114. The pump connection hole 114 can be disposed on the upper side of the valve connection portion 223. The pump connection hole 114 can be disposed on the upper side of the pump receiving groove 222a. The pump connection hole 114 is formed into a circular hole shape, and the pump knob 910 can be disposed through the valve connection hole 113. The pump knob 910 can rotate within the pump connection hole 114.

[0086] A support protrusion 115 for supporting the printed circuit board 500 is formed on the upper surface of the upper housing 110, protruding inward toward the housing 100. The support protrusion 115 can be disposed on the vertical upper side of the substrate joint portion 221 of the frame 200. The support protrusion 115 contacts the upper surface of the printed circuit board 500 and supports the printed circuit board 500.

[0087] A second vent 116 is also formed on the upper surface of the upper outer casing 110. The second vent 116 can be disposed on the vertical upper part of the waste solution tank 330. The second vent 116 can communicate with the internal space of the waste solution tank 330. When the buffer solution or sample solution flows into the waste solution tank 330, the air inside the waste solution tank 330 can be discharged to the outside through the second vent 116. This prevents the internal air pressure of the waste solution tank 330 from rising.

[0088] A second air passage 116a is also formed on the upper surface of the upper housing 110. The second air passage 116a is formed to communicate with the second vent 116 and is formed in the shape of a groove along the upper surface of the upper housing 110. The second air passage 116a can be formed not only as a straight groove, but also as a shape that bends multiple times on the upper surface of the upper housing 110. This is to ensure the maximum flow path length within a limited area. With this structure, when the label 130 is adhered to the upper surface of the upper housing 110, the second air passage 116a forms a space between the upper housing 110 and the label 130 to accommodate the air discharged through the second vent 116.

[0089] The lower outer shell 120 is combined with the upper outer shell 110 to form a space inside that can accommodate the frame 200, the tank 300, the biosensor 400, the printed circuit board 500, the channel 600, the tube 700, the valve section 800, and the pump section 900.

[0090] As one embodiment, the lower housing 120 is formed as a four-cornered box with an open upper side, which can be combined with the upper housing 110. On the side wall of the lower housing 120, a plurality of hooks are formed protruding toward the upper housing 110 and can be fitted into grooves (not shown) formed on the side wall of the upper housing 110 for combination.

[0091] A sensor insertion hole 121 for inserting a biosensor 400 may be formed on the lower surface of the lower housing 120. As one embodiment, the sensor insertion hole 121 is formed in a circular shape, and a pair of cap fittings 121a are formed radially inwardly on at least a portion of the inner circumferential surface. The cap fittings 121a are supported by hooks 122a of the sensor cap 122.

[0092] A sensor cover 122 may be attached to the lower surface of the lower housing 120. The sensor cover 122 may cover the sensor insertion hole 121 and may prevent the biosensor 400 attached to the frame 200 from being detached.

[0093] The sensor cover 122 may include a hook 122a, a sensor support portion 122b, and a coupling guide portion 122c. The sensor cover 122 is generally formed in a disk shape. The hook 122a and the sensor support portion 122b are protrudingly formed on the upper surface of the sensor cover 122, and a coupling guide groove 122c is recessed on the lower surface of the sensor cover 122. The diameter of the sensor cover 122 may correspond to the diameter of the sensor insertion hole 121. As an embodiment, the diameter of the sensor cover 122 may be the same as the diameter of the sensor insertion hole 121.

[0094] Hooks 122a are formed protruding upwards from the upper surface of the sensor cover 122, with their upper ends protruding radially outwards. A pair of hooks 122a are formed in opposite positions and at a predetermined angle along the circumferential direction. After the hooks 122a are inserted into the sensor insertion hole 121 where the cover joint 121a is not formed, they can be positioned on the upper side of the cover joint 121a when the sensor cover 122 is rotated. Thus, by positioning the cover joint 121a between the hooks 122a and the upper surface of the sensor cover 122, the sensor cover 122 can be prevented from detaching.

[0095] The sensor support portion 122b is formed protruding upward along the circumferential direction on the upper surface of the sensor cover 122. The sensor support portion 122b is protrudingly formed as a circular rib with the radial center of the sensor cover 122 as its origin. The sensor support portion 122b is formed to contact and support the lower surface of the biosensor 400. When the sensor cover 122 is attached to the lower housing 120 and blocks the sensor insertion hole 121, the sensor support portion 122b can contact and support the biosensor 400. This prevents the biosensor 400 from shaking, thereby preventing errors in the measurement values ​​of the biosensor 400.

[0096] Figure 6 This is an exploded perspective view used to illustrate the framework of one embodiment of the present invention. Figure 7 This is a top view used to illustrate the upper frame of one embodiment of the present invention. Figure 8 This is a cross-sectional view of the frame according to an embodiment of the present invention.

[0097] Reference Figures 6 to 8 The frame 200 is disposed inside the housing 100, and channels 600 for the flow of buffer solution and sample solution can be formed inside.

[0098] The biosensor 400 and the printed circuit board 500 can be detachably attached to the frame 200, so that the biosensor 400 can detect biological substances in the sample solution flowing in the channel 600.

[0099] The frame 200 may include a bottom frame 210, an upper frame 220, a hydrophilic adhesive layer 230, and a micro-flow path forming adhesive layer 240. As one embodiment, the hydrophilic adhesive layer 230 is stacked on the upper side of the bottom frame 210, the micro-flow path forming adhesive layer 240 is stacked on the upper side of the hydrophilic adhesive layer 230, and the upper frame 220 may be stacked on the upper side of the micro-flow path forming adhesive layer 240.

[0100] The biosensor 400 can be detachably attached to the bottom frame 210. The bottom frame 210 is attached to the biosensor 400 and can support the biosensor 400. As one embodiment, the bottom frame 210 can be formed in the shape of a generally four-cornered flat plate and can be formed with a sensor attachment 211 for attaching to the biosensor 400.

[0101] The sensor connector 211 can be detachably coupled to the biosensor 400. The sensor connector 211 is generally formed with a hole shape corresponding to the shape of the biosensor 400, and may have a frame extending transversely through the hole along its minor axis. A pair of ports 631 and 632 may be formed in the frame. As an embodiment, the sensor connector 211 is formed with a quadrangular hole shape, and its diameter in the front-rear direction and its diameter in the left-right direction may be formed to be the same as the length in the front-rear direction and the width in the left-right direction of the biosensor 400.

[0102] The biosensor 400 can be fitted into the sensor junction 211. At least a portion of the sidewall surrounding the sensor junction 211 can be greater than the thickness of the biosensor 400. Thus, the biosensor 400 can be stably supported when fitted into the sensor junction 211.

[0103] The bottom frame 210 may have holes for fixing the valve part 800 and the pump part 900, and through holes for fixing components such as screws, so that it can be combined with the valve part 800 and the pump part 900 disposed on the upper side of the upper frame 220.

[0104] A can 300 may be formed on the upper frame 220. As an example, the can 300 may be formed on the upper surface of the upper frame 220.

[0105] A channel 600 may be formed in the upper frame 220. As an example, a channel 600 may be formed on the lower surface of the upper frame 220.

[0106] The upper frame 220 can be formed of a resin material. As an example, the upper frame 220 can be formed of PMMA (Polymethyl methacrylate) resin. This allows for the formation of fine channels 600 through injection molding and enables mass production. Furthermore, the upper frame 220 and the bottom frame 210 can be easily bonded together using a tape.

[0107] A substrate bonding portion 221 may be formed on the upper surface of the upper frame 220. A printed circuit board 500 is detachably bonded to the substrate bonding portion 221. The substrate bonding portion 221 may be positioned opposite to the sensor bonding portion 211. That is, at least a portion of the substrate bonding portion 221 may be positioned above the sensor bonding portion 211. This minimizes the distance between the biosensor 400 and the printed circuit board 500, allowing information sensed by the biosensor 400 to be transmitted quickly and accurately to the printed circuit board 500.

[0108] The substrate bonding portion 221 may include bonding guide portions 221a that guide the sliding bonding of the printed circuit board 500. The bonding guide portions 221a protrude upwards from the upper surface of the upper frame 220 and are formed as a pair, then bend and extend in opposite directions. As an embodiment, the spacing between the pair of bonding guide portions 221a may be the same as the width of the printed circuit board 500. The pair of bonding guide portions 221a can stably support the printed circuit board 500 and prevent the printed circuit board 500 from wobbling in the horizontal direction. As an embodiment, the protrusion height of the pair of bonding guide portions 221a is the same as or slightly greater than the thickness of the printed circuit board 500. This prevents the printed circuit board 500 from moving in the vertical direction.

[0109] The substrate bonding portion 221 may further include a substrate support portion 221b that guides the bonding position of the printed circuit board 500 and supports the printed circuit board 500. A pair of opposing substrate support portions 221b are formed protruding upwards from the upper surface of the upper frame 220. The substrate support portions 221b may be formed corresponding to the shape of the two ends in the width direction (minor axis direction) of the printed circuit board 500. As an embodiment, when semi-circular grooves are formed at both ends in the width direction of the printed circuit board 500, the pair of substrate support portions 221b may be formed in a semi-circular protrusion towards each other. As an embodiment, the shortest distance between the pair of substrate support portions 221b may be the same as the shortest distance in the width direction of the printed circuit board 500. Thus, when the printed circuit board 500 is bonded, it is fitted with the substrate support portions 221b to guide the bonding position, preventing the printed circuit board 500 from moving along the insertion direction.

[0110] As a result, the substrate bonding portion 221 can prevent the printed circuit board 500, which is bonded by the bonding guide portion 221a and the substrate support portion 221b, from wobbling in the horizontal and vertical directions. This has the effect that the substrate bonding portion 221 stably supports the bonded printed circuit board 500, thereby preventing the printed circuit board 500 from wobbling and causing data errors.

[0111] A clamping hole 221c for receiving the contact clip 450 can be formed in the substrate bonding portion 221. A pair of clamping holes 221c can be disposed on the substrate bonding portion 221. As an embodiment, at least a portion of one of the clamping holes 221c can be disposed between a pair of bonding guide portions 221a, and at least a portion of the other clamping hole 221c can be disposed between a pair of substrate support portions 221b. This prevents the contact clip 450 housed in the clamping hole 221c from dislodging from its intended position.

[0112] A pair of clip receiving holes 221c can be formed to communicate with the sensor coupling portion 211. At least a portion of each of the pair of clip receiving holes 221c can be disposed at a position opposite to the sensor coupling portion 211. As an embodiment, the sensor coupling portion 211 is disposed between the pair of clip receiving holes 221c, and portions of both ends of the sensor coupling portion 211 in the longitudinal direction (front-back direction) can be disposed overlapping with at least a portion of each of the pair of clip receiving holes 221c. With such a structure, a step for engaging the contact clip 450 can be formed when the bottom frame 210 and the upper frame 220 are stacked. As a result, the contact clip 450 itself can directly contact the biosensor 400 and the printed circuit board 500, thereby improving the accuracy of data transmission through the contact clip 450.

[0113] A tube receiving portion 222 may be formed in the upper frame 220. The tube receiving portion 222 is formed protruding upward on the upper surface of the upper frame 220. The tube receiving portion 222 can guide the arrangement position of the tube 700 on the upper frame 220.

[0114] The pipe receiving section 222 is formed in a block shape on the upper surface of the upper frame 220, and a pump receiving groove 222a and a pipe guiding groove 222b can be formed inside it.

[0115] The pump receiving groove 222a internally houses at least a portion of the tube 700 and the pump section 900. The tube 700 is arranged circumferentially, and at least a portion of the pump section 900 is rotatably housed inside the wound tube 700. At least a portion of the pump section 900 is arranged in the pump receiving groove 222a in a state of contact with the tube 700. As an embodiment, the pump receiving groove 222a is formed into a circular groove shape, the tube 700 is wound along its inner circumferential surface, and the pump section 900 is rotatably arranged inside the tube 700. When the pump section 900 rotates, the tube 700 disposed between the side wall of the pump receiving groove 222a and the pump section 900 can be compressed.

[0116] The tube guide groove 222b is configured to communicate with the pump receiving groove 222a and can receive at least a portion of the tube 700. One side and the other side of the wound tube 700 at the pump receiving groove 222a can be received in the tube guide groove 222b respectively. One side and the other side of the tube 700 are received in the tube guide groove 222b in a crisscrossing manner. That is, the groove for receiving one side of the tube 700 and the groove for receiving the other side of the tube 700 merge into one, and the merging point 222c can communicate with the pump receiving groove 222a. Thus, when the pump section 900 rotates, at least a portion of the tube 700 can remain in contact with the pump section 900, and backflow in the solution flowing in the tube 700 can be prevented when the pump section 900 rotates.

[0117] A valve engagement portion 223 may be formed on the upper frame 220. The valve engagement portion 223 may be formed protruding upward on the upper surface of the upper frame 220. As an embodiment, the valve engagement portion 223 may be formed protruding in the form of a rib on the upper surface of the upper frame 220. The valve engagement portion 223 may be formed to surround at least a portion of the outer side of the valve portion 800. Thus, the valve engagement portion 223 may guide the engagement position of the valve portion 800.

[0118] Multiple ports may be formed in the upper frame 220. These ports provide space for buffer solution or sample solution to flow into the channel 600 or to drain from the channel 600. The upper frame 220 may include a buffer solution inflow port 315, a buffer solution port 615, a sample solution port 625, a sensing port 635, a pre-fill port 645, a waste solution port 655, a first tube connection port 660, and a second tube connection port 670. The buffer solution inflow port 315 is disposed within the buffer solution tank 310 to allow the buffer solution within the buffer solution tank 310 to flow into the channel 600.

[0119] The buffer solution port 615, sample solution port 625, sensing port 635, and pre-filling port 645 are configured to be connected via valve section 800. As one embodiment, the buffer solution port 615, sample solution port 625, and pre-filling port 645 can be arranged on concentric circles with sensing port 635 as the origin. In this case, the buffer solution port 615, sample solution port 625, and pre-filling port 645 are arranged with a predetermined angular difference relative to sensing port 635 as the origin.

[0120] The first pipe connection port 660 and the second pipe connection port 670 can be configured to connect to the pipe 700. The first pipe connection port 660 and the second pipe connection port 670 are arranged adjacent to the pump section 900. As one embodiment, the second pipe connection port 670 is arranged on one side of the upper frame 220 along the length direction with reference to the pump section 900, and the first pipe connection port 660 is arranged on the other side of the upper frame 220 along the length direction with reference to the pump section 900.

[0121] In the case of conventional biosensor boxes, channels are formed inside the frame to allow liquid to flow. However, the accuracy of the sensing decreases each time a sample solution is tested because the flow rate of the solution is not constant.

[0122] To address this issue, a hydrophilic coating is sometimes applied to the bottom frame. However, this increases production costs due to the added coating process and also has the drawback of increasing the defect rate due to uneven coating.

[0123] Therefore, in this invention, the flow rate of the solution is kept constant by means of the hydrophilic adhesive layer 230, while the rate of increase in production costs and the rate of defective products are minimized.

[0124] A hydrophilic adhesive layer 230 may be disposed between the bottom frame 210 and the upper frame 220. The hydrophilic adhesive layer 230 may also be disposed between the bottom frame 210 and the micro-flow path forming adhesive layer 240. For example, the hydrophilic adhesive layer 230 may be a hydrophilic tape or a hydrophilic film. The hydrophilic adhesive layer 230 is disposed on the upper side of the bottom frame 210 to facilitate the flow of fluid in the channel 600. Furthermore, it has the effect that the flow rate of the solution can be stably maintained by the hydrophilic adhesive layer 230. Additionally, it has the advantage that even during the manufacturing process, bonding can be achieved through a simple process of placing the hydrophilic adhesive layer 230, the micro-flow path forming adhesive layer 240, and the upper frame 220 on the upper side of the bottom frame 210 and applying pressure, without the need for additional heating steps.

[0125] The hydrophilic adhesive layer 230 can be formed in a shape corresponding to the shape of the upper frame 220. As an example, a clamping hole 231 can be formed in the hydrophilic adhesive layer 230. The clamping hole 231 of the hydrophilic adhesive layer 230 can be formed at a position opposite to the clamping hole 221c of the upper frame 220, and can be formed in the same size and shape.

[0126] The hydrophilic adhesive layer 230 can be formed into a shape corresponding to the shape of the bottom frame 210. As an example, ports can be formed in the hydrophilic adhesive layer 230. The ports of the hydrophilic adhesive layer 230 can be formed at positions opposite to the ports 631a and 632a of the bottom frame 210, and can be formed into the same size shape.

[0127] The micro-flow path forming adhesive layer 240 can be disposed between the hydrophilic adhesive layer 230 and the upper frame 220. The micro-flow path forming adhesive layer 240 can bond the hydrophilic adhesive layer 230 and the upper frame 220. The micro-flow path forming adhesive layer 240 can be formed into a shape corresponding to the shape of the upper frame 220.

[0128] The micro-flow path forming adhesive layer 240 can form channel gaps 242 communicating with the channels 600 formed on the upper frame 220. The channel gaps 242 can be formed at a position opposite to the channels 600. As an example, the width of the channel gaps 242 can be greater than the width of the channels 600. Therefore, even if there are errors in the positions of the channels 600 and the channel gaps 242, errors in the flow of the solution can be prevented.

[0129] A clamping hole 241 can be formed in the adhesive layer 240 formed in the microflow path. The clamping hole 241 of the adhesive layer 240 formed in the microflow path is formed at a position opposite to the clamping hole 221c of the upper frame 220, and can be formed in the same size shape.

[0130] The container 300 is formed on the upper surface of the frame 200 and provides space for receiving buffer solutions and / or sample solutions. As one embodiment, the container 300 may be formed in a shape that protrudes from the upper surface of the upper frame 220 and surrounds a defined space.

[0131] Tank 300 may include buffer solution tank 310. Buffer solution flows into buffer solution tank 310, which at least temporarily holds the buffer solution, allowing the buffer solution to flow into channel 600.

[0132] The buffer solution tank 310 can be disposed on the rear side (one side in the length direction) of the frame 200. The buffer solution tank 310 can also be disposed on the opposite side of the substrate joint 221 on the frame 200.

[0133] The buffer solution tank 310 can be configured on the left side (one side in the direction of the short axis) of the frame 200. The buffer solution tank 310 can also be configured on the opposite side of the waste solution tank 330 on the frame 200.

[0134] The buffer solution tank 310 is formed protrudingly as a wall on the upper surface of the upper frame 220. As one embodiment, the buffer solution tank 310 is formed as a quadrangular wall. A buffer solution inlet port 315 may be formed in the upper frame 220, and the buffer solution inlet port 315 may be configured to communicate with the buffer solution channel 610. The buffer solution inlet port 315 may be formed within the buffer solution tank 310.

[0135] An inclined surface 311 and a guide groove are formed within the buffer solution tank 310 to guide the buffer solution flow to the buffer solution inlet port 315. As one embodiment, the inclined surface is formed by sloping downwards from the rear end of the buffer solution tank 310 towards the front. The inclined surface is also formed by sloping downwards from both sides of the buffer solution tank 310 towards the center in the left-right direction. A guide groove is formed along the front-back direction on the inner bottom surface of the buffer solution tank 310. The buffer solution inlet port 315 is formed on the guide groove.

[0136] The buffer solution tank 310 surrounds the space in which the buffer solution flows. Thus, the buffer solution can be contained in the buffer solution tank 310 and can be discharged into the channel 600 as the pump unit 900 operates.

[0137] The container 300 may include a sample solution container 320. The sample solution is flowed into the sample solution container 320, which can at least temporarily hold the sample solution and allow the sample solution to flow into the channel 600.

[0138] The sample solution container 320 can be disposed on the rear side of the frame 200. The sample solution container 320 can also be disposed on the opposite side of the substrate bonding portion 221 on the frame 200.

[0139] At least a portion of the sample solution container 320 may be disposed in the central part of the frame 200 in the left-right direction. The sample solution container 320 may be disposed between the buffer solution container 310 and the waste solution container 330.

[0140] The sample solution container 320 is formed protrudingly as a wall on the upper surface of the upper frame 220. As one embodiment, the sample solution container 320 is formed protruding as a circular wall. The upper frame 220 may be configured such that at least a portion of the lower side of the internal space of the sample solution container 320 is open. The internal space of the sample solution container 320 may be configured to communicate with the sample solution channel 620.

[0141] The sample solution container 320 surrounds the space in which the sample solution flows. Thus, the sample solution can be contained in the sample solution container 320, and can be discharged into the channel 600 by the operation of the pump unit 900.

[0142] Tank 300 may include waste solution tank 330. Waste solution may flow into waste solution tank 330 for storage.

[0143] Waste solution tank 330 can be disposed on the rear side of frame 200. Waste solution tank 330 can be disposed on the opposite side of substrate joint 221 on frame 200. Waste solution tank 330 can be disposed on the right side of frame 200.

[0144] The waste solution tank 330 is formed protrudingly as a wall on the upper surface of the upper frame 220. As one embodiment, the waste solution tank 330 is formed as a quadrangular wall. A waste solution port 655 may be formed in the upper frame 220, and the waste solution port 655 may be configured to communicate with the waste solution channel 650. The waste solution port 655 may be formed within the waste solution tank 330.

[0145] Waste solution tank 330 surrounds the space in which the waste solution flows. With the operation of pump 900, the waste solution can flow from channel 600 into waste solution tank 330 for storage.

[0146] Figure 9 This is a bottom view used to illustrate a channel formed on the upper frame of an embodiment of the present invention.

[0147] Reference Figure 9 Channel 600 is formed inside frame 200 and provides a flow path for buffer solution or sample solution to flow. Channel 600 may be formed on the lower surface of upper frame 220.

[0148] Channel 600 includes a buffer solution channel 610 connected to the buffer solution tank 310 and for supplying buffer solution flow. One side of the buffer solution channel 610 is in communication with the buffer solution inlet port 315. Another side of the buffer solution channel 610 is in communication with the internal space of the buffer solution tank 310 via the buffer solution inlet port 315. The other side of the buffer solution channel 610 is in communication with the buffer solution port 615. With the operation of the valve section 800, the other side of the buffer solution channel 610 is in communication with the sensing channel 630 via the buffer solution port 615.

[0149] The buffer solution channel 610 can guide the buffer solution flowing into the buffer solution tank 310 to the valve section 800. As one embodiment, the buffer solution channel 610 can be formed along the length direction (major axis direction) of the frame 200.

[0150] The buffer solution channel 610 can be formed in a shape that is bent at least once at a predetermined angle. As one embodiment, the buffer solution channel 610 is formed generally along the length direction (major axis direction) of the frame 200, and is formed with a portion that is bent twice.

[0151] The buffer solution inlet port 315 can serve as the inlet of the buffer solution channel 610, and the buffer solution outlet port 615 can serve as the outlet of the buffer solution channel 610.

[0152] The buffer solution channel 610 may be configured such that the width of the inlet side and the width of the outlet side are different. As an example, the buffer solution channel 610 may be configured such that the width of the outlet side is greater than the width of the inlet side. Therefore, the buffer solution can be sufficiently present on the outlet side of the buffer solution channel 610, preventing a decrease in the flow rate of the buffer solution through the valve section 800, and effectively maintaining a stable flow rate of the buffer solution.

[0153] Channel 600 includes a sample solution channel 620 connected to the sample solution container 320 and for supplying sample solution flow. One side of the sample solution channel 620 is in communication with the internal space of the sample solution container 320. The other side of the sample solution channel 620 is in communication with the sample solution port 625. With the operation of the valve section 800, the other side of the sample solution channel 620 can be connected to the sensing channel 630 through the sample solution port 625.

[0154] The sample solution channel 620 guides the sample solution flowing into the sample solution container 320 to the valve section 800. As an example, the sample solution channel 620 is formed from the sample solution container 320 along a direction intersecting the length direction (major axis direction) of the frame 200, then bent and formed along the length direction of the frame 200, and then bent and formed along a direction intersecting the length direction. The internal space of the sample solution container 320 serves as the inlet of the sample solution channel 620, and the sample solution port 625 serves as the outlet of the sample solution channel 620.

[0155] The diameter of the internal space of the sample solution container 320 may be greater than the width of the outlet side of the sample solution channel 620. The width of at least a portion of the sample solution channel 620 may vary. As an example, the sample solution channel 620 is configured such that the width of the inlet side and the width of the outlet side are the same, with a narrowing section in between. This prevents the flow rate of the sample solution flowing in the sample solution channel 620 from momentarily decreasing with the operation of the pump unit 900, thus maintaining a stable flow rate of the sample solution.

[0156] Channel 600 may include a sensing channel 630 connected to buffer solution channel 610 or sample solution channel 620 and guiding the buffer solution or sample solution to biosensor 400.

[0157] One side of the sensing channel 630 can be connected to the sensing port 635. The other side of the sensing channel 630 can be connected to the first pipe connection port 660. As the valve section 800 operates, the other side of the sensing channel 630 can be connected to the flow path formed inside the pipe 700 through the first pipe connection port 660.

[0158] The sensing channel 630 guides the buffer solution or sample solution flowing in through the valve section 800 to the biosensor 400, allowing it to pass through the biosensor 400.

[0159] The sensing channel 630 may include a first sensing channel 631 and a second sensing channel 632.

[0160] The first sensing channel 631 guides the buffer solution or sample solution that has passed through the valve section 800 to the biosensor 400. The first sensing channel 631 is formed from the sensing channel 630 along the length direction (major axis direction) of the frame 200, then bent and formed in a direction intersecting the length direction. As an embodiment, the first sensing channel 631 may be formed from the sensing port 635 along the length direction (major axis direction) of the frame 200, then bent and formed in the width direction. The other side of the first sensing channel 631 may communicate with the internal space of the sensor junction 211. The first sensing channel 631 allows the buffer solution or sample solution to flow through the inflow port 631a formed in the bottom frame 210 into the internal space of the sensor junction 211.

[0161] The buffer solution or sample solution passing through the first sensing channel 631 can flow on the upper surface of the biosensor 400.

[0162] The sensing port 635 can serve as the inlet of the first sensing channel 631, and the sensor junction 211 can serve as the outlet of the first sensing channel 631.

[0163] The width of the inlet side of the first sensing channel 631 can be greater than the width of the outlet side of the first sensing channel 631. Therefore, the buffer solution or sample solution can be sufficiently present on the inlet side of the first sensing channel 631, preventing the flow rate of the buffer solution or sample solution flowing in the first sensing channel 631 from dropping instantaneously with the operation of the pump unit 900, and thus maintaining a stable flow rate of the buffer solution or sample solution.

[0164] The second sensing channel 632 can guide the buffer solution or sample solution that has passed through the biosensor 400 to the tube 700. As an example, the second sensing channel 632 can communicate with the discharge port 632a formed in the bottom frame 210 and is formed along the left-right direction to communicate with the first tube connection port 660. The second sensing channel 632 can be aligned in a straight line with the downstream side of the first sensing channel 631. By forming the flow path through the biosensor 400 in a straight line, the flow rate and / or flow volume of the buffer solution or sample solution flowing in the biosensor 400 can be stably maintained, improving the accuracy of sensing biological substances.

[0165] Channel 600 may include a pre-filled channel 640 communicating with sample solution channel 620 for sample solution flow. One side of pre-filled channel 640 may communicate with sample solution channel 620. One side of pre-filled channel 640 may communicate with sample solution port 625. The other side of pre-filled channel 640 may communicate with pre-filled port 645.

[0166] The pre-fill channel 640 can guide the sample solution that has passed through the sample solution channel 620 to the pre-fill port 645. As one embodiment, the pre-fill channel 640 may be configured to communicate with the sample solution channel 620 and be bent multiple times to communicate with the pre-fill port 645.

[0167] At this time, the width of at least a portion of the pre-filling channel 640 may change. As an example, the width of the inlet side and the outlet side of the pre-filling channel 640 are the same, while there is a narrowing interval in between.

[0168] A valve 641 with a width greater than that of the inlet and outlet can be provided in the pre-filling channel 640. The valve 641 has the largest width in the pre-filling channel 640, and the inlet width into which the sample solution flows is the narrowest in the pre-filling channel 640. Therefore, in the event of a large amount of sample solution flowing into the pre-filling channel 640 instantaneously, it is possible to prevent the sample solution from being discharged into the pre-filling port 645.

[0169] Channel 600 may include a waste solution channel 650 that guides the buffer solution or sample solution that has passed through tube 700 to waste solution tank 330. Specifically, one side of waste solution channel 650 may be connected to a second tube connection port 670. The other side of waste solution channel 650 may be connected to a waste solution port 655. When pump unit 900 is running, the buffer solution or sample solution that has passed through tube 700 is stored in waste solution tank 330.

[0170] Figure 10 This is a diagram illustrating the integration of a biosensor and a printed circuit board into a frame in a biosensor box according to an embodiment of the present invention. Figure 11 This is a diagram illustrating the sensor junction in a biosensor box according to an embodiment of the present invention. Figure 12 This is a diagram illustrating the state of the biosensor integrated in a biosensor box according to an embodiment of the present invention. Figure 13 This is a cross-sectional view illustrating the contact between the biosensor and the printed circuit board in a biosensor box according to an embodiment of the present invention. Figure 14 and Figure 15 This is a cross-sectional view used to illustrate the configuration of the biosensor and printed circuit board in a biosensor box according to an embodiment of the present invention, as well as the configuration of the flow path flowing on the biosensor.

[0171] Reference Figures 10 to 15 The biosensor 400 can be detachably attached to the frame 200 and can sense biological substances. The biosensor 400 can be inserted through the sensor insertion hole 121 of the lower housing 120 and can be attached to the sensor attachment 211 of the bottom frame 210.

[0172] The biosensor 400 combines a biological receptor that has the function of recognizing specific biological substances with an electrical transducer to convert biological interactions and recognition responses into electrical signals to selectively sense a very small amount of biological substances to be analyzed.

[0173] As one embodiment, a sensing element may be provided on the upper surface of the biosensor 400. A biological receptor may be disposed in the sensing element. A buffer solution and a sample solution may flow in the sensing element. The sensing element is connected to a circuit and can transmit electrical signals generated by the sensing element.

[0174] The biosensor 400 may be configured with circuitry that can be electrically connected to the printed circuit board 500 via a contact clip 450.

[0175] A seal 410 may be disposed on the upper side of the biosensor 400. The seal 410 may be disposed at the sensor junction 211. A flow path forming portion 411 may be formed in the seal 410. As an embodiment, the seal 410 may be formed in a cuboid shape, and the flow path forming portion 411 may be a slit shape formed along the left-right direction.

[0176] The flow path forming section 411 can be disposed on the lower side of the frame formed in the sensor junction 211. The flow path forming section 411 is disposed on the lower side of the inflow port 631a and the discharge port 632a formed in the sensor junction 211, and can communicate with the inflow port 631a and the discharge port 632a. The buffer solution and sample solution flowing in the first sensing channel 631 flow through the inflow port 631a in the flow path inside the flow path forming section 411, and can flow into the second sensing channel 632 through the discharge port 632a.

[0177] The flow path forming portion 411 may be disposed on the upper side of the sensing portion of the biosensor 400. The seal 410 may be formed in a shape that surrounds the outer contour of the sensing portion. The seal 410 can achieve an airtight seal on the outer contour of the flow path forming portion 411, and can prevent the buffer solution and sample solution flowing inside the flow path forming portion 411 from flowing out to the outside.

[0178] The printed circuit board 500 can be detachably attached to the frame 200. At least a portion of the biosensor 400 can be inserted into the upper housing 110 and detachably attached to the substrate attachment portion 221 of the upper frame 220. The printed circuit board 500 can be supported by the attachment guide portion 221a and the substrate support portion 221b.

[0179] The printed circuit board 500 may include a board body 510, a connector 520, and a guide portion 530. As one embodiment, the board body 510 may be generally formed in a rectangular flat plate shape. Circuitry is mounted on the board body 510 for electrical connection to the biosensor 400.

[0180] A connector 520 may be provided at the front end of the substrate body 510. The connector 520 can be connected to a circuit disposed on the substrate body 510. When the biosensor box 1 is combined with the diagnostic device 2, an electrical signal can be sent to the diagnostic device 2 through the connector 520. When the biosensor box 1 is combined with the diagnostic device 2, power can be applied to the printed circuit board 500 and the biosensor 400 through the connector 520.

[0181] The guide portion 530 may be formed at both ends of the substrate body 510 in the left-right direction and may be combined with the substrate support portion 221b of the substrate bonding portion 221. As an embodiment, the guide portion 530 is formed in a curved shape at both ends of the substrate body 510 in the left-right direction. Thus, combined with the protruding shape of the substrate support portion 221b, it can guide the printed circuit board 500 to the correct bonding position, and can stably support the printed circuit board 500 when it is bonded.

[0182] A contact clip 450 may be disposed between the biosensor 400 and the printed circuit board 500. The contact clip 450 is coupled to the sensor junction 211 and can contact the printed circuit board 500 and the biosensor 400. The contact clip 450 is disposed through a clip receiving hole 221c and through a hole in the sensor junction 211, and at least a portion of it is supported by the bottom frame 210.

[0183] Multiple contact clips 450 may be provided. An even number of contact clips 450 may be provided. Multiple pairs of contact clips 450 may be arranged side by side, with each pair of contact clips 450 arranged symmetrically at opposite positions. As one embodiment, six contact clips 450 are formed, with three pairs of contact clips 45 arranged side by side, each pair of contact clips 45 being symmetrically arranged at opposite positions.

[0184] The contact clip 450 may be formed of a conductive material. As one embodiment, the contact clip 450 may be formed of a metallic material. Each contact clip 450 may include a substrate contact portion 451, a sensor contact portion 452, and a connecting portion 453.

[0185] The substrate contact portion 451 is configured via a receiving hole 221c. In this configuration, the substrate contact portion 451 can contact a terminal (not shown) disposed on the printed circuit board 500. That is, the substrate contact portion 451 can be electrically connected to a circuit mounted on the printed circuit board 500. As one embodiment, the substrate contact portion 451 can be a plate-shaped component extending in the front-back direction.

[0186] The sensor contact portion 452 is configured through a hole formed in the sensor connection portion 211 and can contact a terminal (not shown) provided on the biosensor 400. That is, the sensor contact portion 452 can be electrically connected to a circuit installed on the biosensor 400. As an embodiment, the sensor contact portion 452 can be in the form of a plate that extends downward from the connection portion 453, is then bent, and extends upward.

[0187] The total height of the contact clip 450 in the vertical direction is longer than the shortest distance between the biosensor 400 and the printed circuit board 500. As a result, when the sensor contact portion 452 contacts the biosensor 400, the sensor contact portion 452 elastically deforms and applies pressure to the biosensor 400, minimizing the contact area with the biosensor 400 while maintaining a firm contact.

[0188] The connecting portion 453 can be formed to connect the substrate contact portion 451 and the sensor contact portion 452. The connecting portion 453 can be formed by bending and extending downward from the substrate contact portion 451, and then bending and extending along the front-back direction, and bending and extending downward to connect with the sensor contact portion 452. The connecting portion 453 can contact the bottom frame 210 and be supported.

[0189] The contact clip 450 is mounted on the frame 200, with its upper side able to contact the printed circuit board 500 and its lower side able to contact the biosensor 400. Through the contact clip 450, while the buffer solution and sample solution flow between the printed circuit board 500 and the biosensor 400, the electrical signal generated by the biosensor 400 is transmitted to the printed circuit board over the shortest distance.

[0190] Figure 16 This is a diagram illustrating the process of diagnosing a sample solution placed in a biosensor cartridge using a diagnostic apparatus according to an embodiment of the present invention.

[0191] Reference Figure 1 , Figure 2 ,and Figure 16 The biosensor box 1 is in a state where the biosensor 400 and the printed circuit board 500 are combined. However, if there is a fault in the biosensor 400 and the printed circuit board 500, the user can replace them.

[0192] The user can inject the buffer solution into the biosensor cartridge 1. The user can inject the buffer solution into the buffer solution inlet 111. In this invention, a buffer blister 3 is used to inject the buffer solution into the buffer solution inlet 111.

[0193] As one embodiment, the upper part of the blister pack 3 can be formed in a dome shape, and the lower part can be formed in a planar shape. The upper part of the blister pack 3 is formed of a deformable material, and the lower part of the blister pack 3 is formed of a tearable material.

[0194] The buffer blister 3 can be stored in the blister storage slot 111a. The buffer blister 3 can not only be fixed to the blister storage slot 111a, but the buffer blister 3 can also be detachably attached to the blister storage slot 111a.

[0195] With the blister pack 3 positioned inside the blister pack storage slot 111a, the user applies downward pressure to the blister pack 3 using their fingers or the like. In this case, the upper part of the dome-shaped blister pack 3 deforms downward, the internal pressure of the blister pack 3 increases and breaks through the lower surface, and the buffer solution stored inside the blister pack 3 flows downward due to gravity and into the buffer solution inlet 111.

[0196] The buffer solution that has passed through the buffer solution inlet hole 111 can be collected in the buffer solution tank 310. It can also flow along the inclined surface 311 into the buffer solution inlet port 315. Furthermore, a portion of the buffer solution collected in the buffer solution tank 310 can flow along the buffer solution channel 610. However, when the pump unit 900 is not operating, it does not flow into the sensing channel 630.

[0197] The user can inject the sample solution into the sample solution inlet 112. The user can inject the sample solution into the sample solution inlet 112 using a sample delivery tool 4, including a dropper.

[0198] The buffer solution and the sample solution can be injected simultaneously, or the sample solution can be injected first and then the buffer solution, or the buffer solution can be injected first and then the sample solution.

[0199] The sample solution that has passed through the sample solution inlet 112 can be collected into the sample solution container 320. Furthermore, a portion of the sample solution collected in the sample solution container 320 flows along the sample solution channel 620. However, if the pump 900 is not operating, it may not flow into the sensing channel 630.

[0200] After adding the buffer solution and sample solution, the user can insert the biosensor box 1 into the diagnostic device 2.

[0201] Figure 17 This is a cross-sectional view illustrating a solution flow biosensor according to an embodiment of the present invention. Figures 18 to 20 This is a diagram illustrating the circuit configuration of a biosensor according to an embodiment of the present invention.

[0202] Reference Figures 17 to 19 A biosensor 400 according to one embodiment of the present invention may include: a substrate 401, a first gate 421, a second gate 422, a potential measurement unit 423, a first drain 431, a first source 432, a first drain-source channel 433, a second drain 441, a second source 442, a second drain-source channel 443, a third drain 461, a third source 462, a third drain-source channel 463, a fourth drain 471, a fourth source 472, a fourth drain-source channel 473, a first gate electrode 481, a second gate electrode 482, a potential measurement unit electrode 483, a first drain electrode 484, a second drain electrode 485, a third drain electrode 486, a fourth drain electrode 487, and a source electrode 488.

[0203] As one embodiment, substrate 401 may be in a single crystal state and may include silicon (Si) material. As another embodiment, substrate 401 may be a substrate that has been thinned through a thinning process.

[0204] The first gate 421 may be formed on the substrate 401. The first gate 421 may be formed at the central position of the substrate 401.

[0205] When incorporated into the biosensor box 1, the first gate 421 can be configured to be formed at the lower position of the inflow port 631a formed in the bottom frame 210 of the biosensor box 1.

[0206] The second gate 422 may be formed on the substrate 401. The second gate 422 may be formed at the central position of the substrate 401. The second gate 422 is formed on the same straight line at a position spaced apart from the first gate 421.

[0207] When combined with the biosensor box 1, the second gate 422 can be configured to be formed at the lower part of the discharge port 632a of the bottom frame 210 of the biosensor box 1.

[0208] The first gate 421 and the second gate 422 may contain polysilicon or metallic materials (e.g., tungsten (W), copper (Cu), aluminum (Al), gold (Au), palladium (Pd)).

[0209] The first gate 421 and the second gate 422 can be formed by photolithography patterning and deposition processes such as CVD.

[0210] A potential measuring unit 423 is formed between the first gate 421 and the second gate 422, and can measure the potential of the first gate 421 and the second gate 422 respectively.

[0211] Channel portions 433, 443, 463, and 473 may be formed between the first gate 421 and the potential measuring section 423, and between the potential measuring section 423 and the second gate 422.

[0212] The first gate 421, the potential measuring section 423, and the second gate 422 can be formed on the same straight line and in a symmetrical sandwich structure. Therefore, a linear electric field is applied from both sides of the first gate 421 and the second gate 422 to the channel portions 433, 443, 463, and 473, thus generating a stable field effect. Furthermore, this structure of the first gate 421, the potential measuring section 423, and the second gate 422 increases the degree of freedom in the fluid movement direction, thereby improving sensing accuracy.

[0213] A first gap 16 may be formed between the first gate 421 and the potential measuring section 423, and a second gap 17 may be formed between the potential measuring section 423 and the second gate 422. As an embodiment, the first gap 16 and the second gap 17 may be identical. This effectively reduces the deviation between the individual channels.

[0214] By placing the first gate 421 at the lower part of the inflow port 631a formed on the bottom frame 210 of the biosensor box 1, and placing the second gate 422 at the lower part of the outlet port 632a of the bottom frame 210 of the biosensor box 1, the potential can be kept constant, thereby reducing measurement noise and improving sensing accuracy.

[0215] The channel may include a first drain-source channel 433, a second drain-source channel 443, a third drain-source channel 463, and a fourth drain-source channel 473.

[0216] The first drain-source channel 433 may be formed between the first drain 431 and the first source 432, and may also be formed between the first gate 421 and the potential measurement section 423.

[0217] The second drain-source channel 443 may be formed between the second drain 441 and the first source 442, and may also be formed between the potential measurement section 423 and the second gate 422.

[0218] The third drain-source channel 463 may be formed between the third drain 461 and the third source 462, and may also be formed between the first gate 421 and the potential measurement section 423.

[0219] The fourth drain-source channel 473 may be formed between the fourth drain 471 and the fourth source 472, and may be formed between the potential measurement section 423 and the second gate 422.

[0220] As one embodiment, the first drain-source channel 433, the second drain-source channel 443, the third drain-source channel 463, and the fourth drain-source channel 473 may include graphene. As one embodiment, the first drain-source channel 433, the second drain-source channel 443, the third drain-source channel 463, and the fourth drain-source channel 473 may be formed by a patterning and graphene deposition process.

[0221] The first drain 431 may be formed on one side of the first drain-source channel 433, and the first source 432 may be formed on the other side of the first drain-source channel 433.

[0222] The second drain 441 can be formed on one side of the second drain-source channel 443, and the second source 442 can be formed on the other side of the second drain-source channel 443.

[0223] The third drain 461 can be formed on one side of the third drain-source channel 463, and the third source 462 can be formed on the other side of the third drain-source channel 463.

[0224] The fourth drain 471 may be formed on one side of the fourth drain-source channel 473, and the fourth source 472 may be formed on the other side of the fourth drain-source channel 473.

[0225] As an example, the first drain 431, the second drain 441, the third drain 461, the fourth drain 471, the first source 432, the second source 442, the third source 462, and the fourth source 472 may include polycrystalline silicon or metallic materials (e.g., tungsten (W), copper (Cu), aluminum (Al), gold (Au), palladium (Pd).

[0226] As an example, the first drain 431, the second drain 441, the third drain 461, the fourth drain 471, the first source 432, the second source 442, the third source 462, and the fourth source 472 can be formed by photolithography patterning and deposition processes such as CVD.

[0227] The first gate electrode 481 may be formed on one corner of the substrate 401 and may be electrically connected to the first gate electrode 421. When combined with the biosensor cartridge 1, the first gate electrode 481 may be electrically connected to the contact clip 450 of the biosensor cartridge 1.

[0228] The second gate electrode 482 may be formed on the other corner of the substrate 401 and may be electrically connected to the second gate electrode 422. When combined with the biosensor cartridge 1, the second gate electrode 482 may be electrically connected to the contact clip 450 of the biosensor cartridge 1.

[0229] The potential measurement electrode 483 can be formed on the substrate 401 and at a position spaced apart from the first gate electrode 481, and can be electrically connected to the potential measurement unit 423. When combined with the biosensor box 1, the potential measurement electrode 483 can be electrically connected to the contact clip 450 of the biosensor box 1.

[0230] The first drain electrode 484 may be formed on the substrate 401 and between the first gate electrode 481 and the potential measurement electrode 483, and may be electrically connected to the first drain electrode 431. When combined with the biosensor box 1, the first drain electrode 484 may be electrically connected to the contact clip 450 of the biosensor box 1.

[0231] The second drain electrode 485 can be formed on the substrate 401 and at a position spaced apart from the potential measurement electrode 483, and can be electrically connected to the second drain electrode 441. When combined with the biosensor box 1, the second drain electrode 485 can be electrically connected to the contact clip 450 of the biosensor box 1.

[0232] The third drain electrode 486 may be formed at a corner of the substrate 401 on the side opposite to the first gate electrode 481 or the second gate electrode 482, and may be electrically connected to the third drain electrode 461. When combined with the biosensor cartridge 1, the third drain electrode 486 may be electrically connected to the contact clip 450 of the biosensor cartridge 1.

[0233] The fourth drain electrode 487 may be formed on the substrate 401 and may be formed at a position spaced apart from the second gate electrode 482, and electrically connected to the fourth drain electrode 471. When combined with the biosensor cartridge 1, the fourth drain electrode 487 may be electrically connected to the contact clip 450 of the biosensor cartridge 1.

[0234] The source electrode 488 may be formed on the substrate 401 and between the third drain electrode 486 and the fourth drain electrode 487, and may be electrically connected to the first source electrode 432, the second source electrode 442, the third source electrode 462 and the fourth source electrode 472. When combined with the biosensor box 1, the source electrode 488 may be electrically connected to the contact clip 450 of the biosensor box 1.

[0235] Reference Figure 20 As an example, the biosensor 400 may also include a potential compensation unit 27.

[0236] One end of the potential compensation unit 27 can be electrically connected to the first gate electrode 418 and the second gate electrode 482, and the other end of the potential compensation unit 27 can be electrically connected to the potential measurement unit electrode 483.

[0237] The potential compensation unit 27 may include a feedback circuit (negative feedback) including a first input terminal (+), a second input terminal (-), and an output terminal. The first input terminal (+) of the potential compensation unit 27 may be electrically connected to a power supply, the second input terminal (-) of the potential compensation unit 27 may be electrically connected to the electrode 483 of the potential measurement unit, and the output terminal of the potential compensation unit 27 may be electrically connected to the first gate electrode 418 and the second gate electrode 482.

[0238] The potential applied to the first gate 421 and the second gate 422 is fed back through the channel portion and the potential measuring portion 423 via fluid.

[0239] The potentials of the first gate 421 and the second gate 422 applied to the channel portions 433, 443, 463, and 473 by the potential compensation unit 27 can always remain constant, thereby reducing measurement noise and improving sensing accuracy.

[0240] In this invention, the potential compensation unit 27 may be included in the biosensor 400, but is not limited thereto; it may be included in the printed circuit board 500 of the biosensor box 1 or in the diagnostic device 2.

[0241] Although not illustrated separately, a biosensor 400 of one embodiment of the present invention may include a substrate, a gate, a potential measurement unit, a first drain, a first source, a first drain-source channel, a second drain, a second source, a second drain-source channel, a gate electrode, a potential measurement unit electrode, a first drain electrode, a second drain electrode, and a source electrode.

[0242] exist Figure 20 In this process, the potential measurement section can be formed on the substrate at positions spaced apart, and can measure the potential of the gate.

[0243] The channel portion may be formed between the gate and the potential measurement portion, and the channel portion may include a first drain-source channel and a second drain-source channel.

[0244] The first drain-source channel can be formed between the gate and the potential measurement section, and the second drain-source channel can be formed between the gate and the potential measurement section, and can be formed at a position separated from the first drain-source channel by a certain distance.

[0245] The gate and the potential measurement section can be formed on the same straight line.

[0246] Figure 21 and Figure 22 This is a graph used to illustrate noise reduction in the transfer curve of an embodiment of the present invention.

[0247] Figure 21This is a graph showing the transfer curve representing the relationship between drain current (ids) and gate voltage (Vgs) in conventional biosensors. Figure 22 This is a graph showing the transfer curve of the biosensor of the present invention.

[0248] Reference Figure 21 and Figure 22 Compared with previous biosensors, the present invention has the effect of significantly reducing transfer curve noise.

[0249] Figure 23 and Figure 24 This is a diagram used to illustrate potential compensation in one embodiment of the present invention.

[0250] Figure 23 This is a diagram showing the gate potential of conventional biosensors. Figure 24 This is a diagram illustrating the gate potential of the biosensor of the present invention.

[0251] Reference Figure 23 and Figure 24 Compared with conventional biosensors, according to the present invention, by means of gate potential compensation, there is no gate-to-gate voltage drop with respect to the system gate potential, and the gate potential can be kept constant at all times, thereby reducing measurement noise and improving sensing accuracy.

[0252] The present invention has been described in detail above through specific embodiments. However, this is only for the purpose of specific description of the present invention. The present invention is not limited thereto, and those skilled in the art can make modifications or improvements within the technical concept of the present invention.

[0253] Simple modifications and alterations to this invention fall within the scope of this invention, and the specific scope of protection of this invention can be clearly understood through the appended claims.

Claims

1. A biosensor, wherein, The biosensor includes: Substrate; A first gate is formed on the aforementioned substrate; A second gate electrode is formed on the aforementioned substrate; and A potential measuring unit is formed between the first gate and the second gate to measure the potential of the first gate and the potential of the second gate. A channel is formed between the first gate and the potential measuring section and between the potential measuring section and the second gate.

2. The biosensor according to claim 1, wherein, The aforementioned channel section includes: A first drain-source channel is formed between the first gate and the potential measurement section. A second drain-source channel is formed between the potential measurement section and the second gate. A third drain-source channel is formed between the first gate and the potential measurement section, and is formed at a position spaced apart from the first drain-source channel; and The fourth drain-source channel is formed between the potential measurement section and the second gate, and is formed at a position spaced apart from the second drain-source channel.

3. The biosensor according to claim 1, wherein, The first gate, the potential measuring unit, and the second gate are formed on the same straight line.

4. The biosensor according to claim 1, wherein, A first gap is formed between the first gate and the potential measuring unit. A second gap is formed between the potential measurement section and the second gate. The first interval mentioned above is the same as the second interval mentioned above.

5. The biosensor of claim 2, wherein, The biosensor also includes: The first drain electrode is formed on one side of the aforementioned first drain source channel; The first source electrode is formed on the other side of the aforementioned first drain-source channel; The second drain is formed on one side of the aforementioned second drain source channel; The second source electrode is formed on the other side of the aforementioned second drain source electrode channel; The third drain is formed on one side of the aforementioned third drain source channel; The third source electrode is formed on the other side of the aforementioned third drain source electrode channel; The fourth drain electrode is formed on one side of the aforementioned fourth drain source channel; and The fourth source electrode is formed on the other side of the aforementioned fourth drain source electrode channel.

6. The biosensor of claim 1, wherein, The biosensor also includes: A first gate electrode, which is connected to the aforementioned first gate; The second gate electrode is connected to the aforementioned second gate; and The electrode of the potential measuring unit is connected to the aforementioned potential measuring unit. The first gate electrode and the second gate electrode are electrically connected to one end of the potential compensation unit, which compensates the potential of the first gate electrode and the potential of the second gate electrode to a preset potential. The electrode of the potential measuring unit is electrically connected to the other end of the potential compensation unit.

7. A biosensor wherein, The biosensor includes: Substrate; A gate electrode, formed on the aforementioned substrate; and A potential measuring section is formed on the substrate at intervals to measure the potential of the gate. A channel is formed between the gate and the potential measurement section.

8. The biosensor according to claim 7, wherein, The aforementioned channel section includes: A first drain-source channel is formed between the gate and the potential measurement section; and The second drain-source channel is formed between the gate and the potential measurement section, and is formed at a position that is spaced apart from the first drain-source channel.

9. The biosensor according to claim 7, wherein, The aforementioned gate and the aforementioned potential measurement section are aligned on the same straight line.

10. A sensing system, wherein, The sensing system includes: A biosensor box that forms a flow path for the solution; A biosensor, configured in the aforementioned biosensor housing; and A diagnostic device that analyzes signals received from the aforementioned biosensors. The aforementioned biosensors include: Substrate; A first gate is formed on the aforementioned substrate; A second gate electrode is formed on the aforementioned substrate; and A potential measuring unit is formed between the first gate and the second gate to measure the potential of the first gate and the potential of the second gate. A channel is formed between the first gate and the potential measuring section and between the potential measuring section and the second gate.

11. The sensing system according to claim 10, wherein, The aforementioned channel section includes: A first drain-source channel is formed between the first gate and the potential measurement section. A second drain-source channel is formed between the potential measurement section and the second gate. A third drain-source channel is formed between the first gate and the potential measurement section, and is formed at a position spaced apart from the first drain-source channel; and The fourth drain-source channel is formed between the potential measurement section and the second gate, and is formed at a position spaced apart from the second drain-source channel.

12. The sensing system according to claim 10, wherein, The first gate, the potential measuring unit, and the second gate are formed in the same straight direction as the direction of solution flow.

13. The sensing system according to claim 10, wherein, A first gap is formed between the first gate and the potential measuring unit. A second gap is formed between the potential measurement section and the second gate. The first interval mentioned above is the same as the second interval mentioned above.

14. The sensing system according to claim 10, wherein, The diagnostic device mentioned above includes: The potential compensation unit compensates for the potential so that the potential of the first gate and the potential of the second gate are maintained at a preset potential.

15. The sensing system according to claim 14, wherein, The aforementioned biosensors also include: A first gate electrode, which is connected to the aforementioned first gate; The second gate electrode is connected to the aforementioned second gate; and The electrode of the potential measuring unit is connected to the aforementioned potential measuring unit. The first gate electrode and the second gate electrode are electrically connected to one end of the potential compensation unit. The electrode of the potential measuring unit is electrically connected to the other end of the potential compensation unit.

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