Gene detection sample low-temperature storage and transfer device

Abstract

The application relates to a low-temperature storage and transportation device for a gene detection sample, and relates to the technical field of gene detection. The low-temperature storage and transportation device comprises a heat preservation box body, a box cover, a sample bearing frame, an elastic support assembly and a temperature control protection mechanism. The sample bearing frame is used for placing a test tube body, the top end of the test tube body is inserted and connected with a sterile sealing cover, the sample bearing frame is placed on the elastic support assembly, an annular temperature control chamber is formed between the sample bearing frame and the inner side wall of the heat preservation box body, and the sterile sealing cover is positioned by being pressed against the inner side of the box cover under the elastic force of the elastic support assembly. During transportation, the temperature control protection mechanism and the elastic support assembly can buffer and position multiple test tube bodies vertically, the risk that the multiple test tube bodies shake or are damaged is reduced, the cooling effect on the test tube bodies is improved through cooperation of the annular temperature control chamber and the temperature control protection mechanism, and the reliability of sample detection is improved.

Description

Technical Field

[0001] This application relates to the technical field of gene detection, and in particular to a cryogenic transport device for gene detection samples. Background Technology

[0002] In the field of gene testing, accurate and stable storage of samples is a key prerequisite for ensuring the reliability of test results.

[0003] Before transporting gene testing samples (such as nucleic acids, blood, tissues, cells, and other bioactive materials) to the laboratory for testing, they are highly susceptible to factors such as temperature fluctuations, environmental microbial contamination, mechanical vibration, and seal failure, which can lead to nucleic acid degradation, protein denaturation, and microbial growth, ultimately resulting in distorted test results and sample invalidation. Gene testing sample transport devices not only need to have conventional insulation and container functions, but also need to meet core requirements such as being dedicated to biological samples, aseptic sealing, constant low temperature, shockproof and leakproof, and maintaining biological stability. They are specialized experimental auxiliary devices in the fields of microbiology and gene testing.

[0004] Currently, the common method for transporting samples is to place them in a foam box and then place ice packs. However, samples are easily affected by vibration and bumps during transport, and may even break. Moreover, it is difficult to guarantee the low-temperature transport conditions of samples using ice packs. Therefore, providing a low-temperature and stable transport device to improve the reliability of sample testing is an urgent problem to be solved. Summary of the Invention

[0005] To improve the reliability of sample testing, this application provides a cryopreservation and transport device for gene testing samples.

[0006] This application provides a cryopreservation and transport device for gene detection samples, which adopts the following technical solution: A cryogenic transport device for gene detection samples includes an insulated box, a box lid, a sample carrier, an elastic support assembly, and a temperature control and protection mechanism. The sample carrier is used to place multiple test tubes containing gene detection samples, and the top of each test tube is fitted with a sterile, flexible, and sealing cap. The sample carrier is placed on the elastic support component and forms an annular temperature control chamber with the inner wall of the insulated box. The sterile sealing cap is pressed against the inner side of the box lid for positioning under the elastic force of the elastic support component. The temperature control protection mechanism is located on the temperature control chamber and is used to support and position the sample carrier, accurately control the temperature of the test tube body at low temperature, and maintain the stability of the sample during transportation.

[0007] By adopting the above technical solution, the gene sample liquid is added to the test tube body, and the test tube body opening is sealed with a sterile sealing cap. Multiple test tube bodies are placed on the sample carrier rack. After placement, the sample carrier rack is placed into the insulated box and positioned on the elastic support component, so that the outer wall of the sample carrier rack and the inner wall of the insulated box form an annular temperature-controlled chamber. Then, the temperature control protection mechanism is placed in the temperature-controlled chamber. The temperature control protection mechanism is used to cool down the sample carrier rack and the test tube body. Finally, the box lid is fixedly connected to the box body, so that the sterile sealing cap is pressed against the box lid for positioning.

[0008] During transportation, the temperature control and protection mechanism can position the sample carrier rack, providing horizontal cushioning for the sample carrier rack and test tubes; the elastic support components, sterile sealing cap, box lid, and insulated box can vertically cushion and position multiple test tubes, thereby providing cushioning for the sample carrier rack in multiple horizontal and vertical directions and reducing the risk of multiple test tubes shaking or even being damaged.

[0009] Meanwhile, the annular temperature control chamber and temperature protection mechanism work together to achieve circumferential temperature distribution around the test tube body, resulting in more uniform circumferential temperature and avoiding the problem of "cold bottom and hot top, large temperature difference" of traditional bottom ice packs, thus significantly improving temperature control stability. Moreover, the temperature control chamber wraps multiple test tube bodies inside, providing all-round protection when external heat penetrates, further improving the cooling effect on the test tube bodies, thereby improving the reliability of sample testing.

[0010] Optionally, the sample holder has a sterile sample cavity and multiple placement holes that communicate with the sterile sample cavity and allow the test tube body to pass through and be placed. Multiple elastic support blocks corresponding to the multiple placement holes are spaced apart in the sterile sample cavity. The support blocks have arc-shaped support grooves for placing and positioning the test tube body. The surface of the support grooves is coated with a medical sterile coating.

[0011] By adopting the above technical solution, the upper part of the test tube body is limited by the placement hole, and the lower part of the test tube body is supported by the arc groove. The two-point positioning and coaxiality are good. At the same time, the contact area with the outer wall of the test tube body is greatly reduced by relying only on the arc groove and the placement hole. This reduces the risk of the sample carrier and the test tube body freezing and sticking at low temperature, ensures smooth loading and unloading of the test tube body, and improves the reliability of sample detection.

[0012] Meanwhile, the bottom of the test tube is supported by an elastic support block, and the elastic sterile sealing cap is pressed against the box lid, which further reduces the risk of damage to the test tube during transportation and the entry of impurities into the sample after damage, thereby further improving the reliability of sample detection.

[0013] Optionally, the sample liquid level inside the test tube body is located below the placement hole, and the sample support rack has multiple first through holes connecting the sterile sample chamber and the temperature control chamber. The temperature control protection mechanism includes: A separator ring is disposed on the upper surface of the sample carrier and is positioned against the sterile sealing cap under the elastic force of the elastic support component. The inner side of the separator ring forms a storage cavity for placing multiple test tubes. The separator ring and the inner wall of the insulated box cooperate to form a ring-shaped low-temperature cold storage cavity with its bottom communicating with the temperature control chamber. The elastic support component has a downwardly recessed sterile placement groove, which cooperates with the sample carrier to form a ventilation space communicating with the sterile placement groove and the temperature control chamber. The sample carrier has air vents communicating with the ventilation space and the sterile sample cavity, and multiple ventilation holes communicating with the storage cavity and the sterile sample cavity. The phase change cold storage and cooling component is filled and placed on the sterile placement tank, temperature control chamber, and low temperature cold storage chamber. It is used to support and position the sample carrier and the separator ring, and to cooperate in the temperature control and cooling treatment of the test tube body.

[0014] By adopting the above technical solution, the sample inside the test tube will not be completely filled. If the entire test tube is cooled, it will be difficult to ensure the most effective cooling of the sample under the same cooling conditions, which will reduce the cooling effect on the sample and thus have an adverse impact on the reliability of sample detection.

[0015] Air enters the sterile sample chamber through the low-temperature cold storage chamber, the temperature control chamber, and the first through hole. At the same time, some air located in the temperature control chamber also enters the sterile sample chamber through the ventilation space and the air vent. When the air passes through, it can be cooled by the phase change cold storage cooling device. The cold air directly cools the sample and reduces the space, allowing the cold air to spread quickly and come into contact with the sample, thus improving the cooling and temperature control effect of the sample. Moreover, the cold air can enter the storage chamber through the ventilation hole to continue cooling the test tube body located above the sample support rack.

[0016] Meanwhile, the phase change cooling devices in the low-temperature cold storage chamber and the temperature control chamber work together to cool the sterile sample chamber, so that the air in the sterile sample chamber is cooled first, and the sample liquid can be cooled directly first. Moreover, by cooling from the bottom and all sides, the cooling effect of the sample can be well guaranteed, and the reliability of sample detection is improved.

[0017] Meanwhile, the separator ring is positioned against the lid, which reduces the force exerted on the sterile seal by the elasticity, thereby further improving the safety of sample transport and the reliability of sample testing.

[0018] Optionally, the diameter of the ventilation hole is smaller than the diameter of the first through hole, and the number of the ventilation holes is less than the sum of the number of the first through hole and the number of the air duct.

[0019] By adopting the above technical solution, the amount of cooling air entering the sterile sample chamber is greater than the amount of air output, which can greatly extend the residence time of air in the sterile sample chamber and enable the cold air to spread in the sterile sample chamber to cool and control the temperature of all samples, thereby further improving the cooling effect and improving the reliability of sample detection.

[0020] Optionally, the lid has a recessed receiving groove, and the separator ring and the sterile sealing cap are positioned by pressing against the bottom of the receiving groove under the elastic force of the elastic support component; the top of the insulated box is provided with a ring-shaped positioning ring that is inserted into the receiving groove for positioning.

[0021] By adopting the above technical solution, the receiving slot is used to accommodate the sample carrier and the separator ring, which are then positioned against it. After the lid is removed, the tops of the sample carrier and the separator ring extend to the outside of the insulated box, making it easy to put in and take out the sample carrier and the separator ring. At the same time, after the positioning ring is inserted and installed on the receiving slot, the bottom of the lid is positioned against the upper surface of the insulated box, thereby achieving a labyrinth seal for the sample carrier and the test tube body. This further improves the safety and convenience of transportation, and enhances the reliability and efficiency of sample testing.

[0022] Optionally, an annular mounting ring is provided on the inner sidewall of the receiving groove, and the separating ring is slidably mounted on the inner sidewall of the mounting ring, and cooperates with the mounting ring and the inner sidewall of the receiving groove to form a sterile drainage cavity; The mounting ring has multiple mounting holes, and each mounting hole is equipped with an air-guiding component with a sterile filter membrane; the partition ring, located above the mounting ring, has multiple second through holes connecting the storage cavity and the sterile drainage cavity. When the air intake device is activated, it drives the gas in the storage chamber to enter the sterile drainage chamber through the second through hole, and then sequentially through the low-temperature cold storage chamber and the temperature control chamber before entering the sterile sample chamber to cool and control the temperature of the sample.

[0023] By adopting the above technical solution, the air intake device is activated, allowing the gas in the storage chamber to enter the sterile drainage chamber through the second through hole. Then, the air passes sequentially through the phase change cold storage cooling device located in the low-temperature cold storage chamber and the temperature control chamber for cooling. Next, the air enters the sterile sample chamber through the second through hole to cool the sample. Then, the air enters the storage chamber through the ventilation hole, thereby realizing the air circulation within the insulation box. At the same time, since the diameter of the ventilation hole is smaller than the diameter of the first through hole, and the number is less than the sum of the number of the first through hole and the air intake hole, the residence time and uniformity of the cold air in the sterile sample chamber can be greatly extended. This allows for better and more uniform cooling of the samples in multiple test tubes, greatly improving the cooling effect on the test tubes and ensuring the reliability of sample testing.

[0024] Meanwhile, the receiving slot is used for the installation of the mounting ring and the exhaust fan. Therefore, after the sample carrier, the separating ring, and the phase change cold storage cooling component are placed, the box cover is then installed on the insulated box. This ensures that the installation ring and exhaust fan do not interfere with the placement of the sample carrier, the separating ring, and the phase change cold storage cooling component, thus facilitating the transfer process. Furthermore, the exhaust fan is located above the phase change cold storage cooling component, reducing the risk of damage to the exhaust fan caused by condensate under gravity. This simultaneously improves the reliability of sample testing and the efficiency of transfer.

[0025] Optionally, it also includes a locking assembly disposed on the insulated box body for locking the box lid, the locking assembly comprising: Two locking blocks are spaced apart on the insulation box body; The locking lever is rotatably mounted on the outer wall of the box cover, has a certain degree of elasticity, and is located between two locking blocks; The locking plate is mounted on the locking rod and presses against the locking block for positioning.

[0026] By adopting the above technical solution, rotating the locking plate closer to the insulated box causes the locking rod to rotate between the two locking blocks, allowing the locking plate to press against the locking blocks for positioning. This achieves the fixed installation of the box cover onto the insulated box. When it needs to be removed, push the locking plate away from the two locking blocks, rotate the locking plate away from the outer wall of the insulated box, and then pull the locking plate to drive the box cover away from the insulated box. This further improves the stability during sample transportation, enhances the reliability of sample testing, and increases the efficiency of transport.

[0027] Optionally, the lower surface of the sample carrier is positioned by being plugged into the elastic support assembly via a connector; the lower surface of the separator ring is positioned by being plugged into the upper surface of the sample carrier via a connector.

[0028] By adopting the above technical solution, the sample carrier is placed vertically on the elastic support assembly, and the first connector is inserted and installed on the elastic support assembly for positioning, forming an annular temperature-controlled chamber between the sample carrier and the inner wall of the insulated box. Then, the phase change cold storage cooling component is placed in the temperature-controlled chamber. Next, the separator ring is placed on the upper surface of the sample carrier, and the second connector is inserted and installed on the sample carrier for positioning, forming an annular low-temperature cold storage chamber between the separator ring and the inner wall of the insulated box. The interior of the separator ring forms a storage cavity, and then the phase change cold storage cooling component is placed in the separator cavity. This facilitates the placement of the phase change cold storage cooling component in the temperature-controlled chamber and the low-temperature cold storage chamber, making the transfer process extremely convenient and improving the sample transfer efficiency.

[0029] Optionally, the separating ring is provided with a support ring that is sleeved on the top of multiple test tube bodies; both the inner walls of the insulated box and the box cover are provided with an insulation layer.

[0030] By adopting the above technical solution, the support ring is fitted on the top of multiple test tube bodies for positioning, so that the top of the test tube body is positioned through the support ring, the middle through the placement hole, and the bottom through the support groove, which can greatly improve the positioning effect of the test tube body and further improve the reliability of sample detection; the heat insulation layer can isolate the external heat, further improving the cooling effect of the sample and improving the reliability of sample detection.

[0031] Optionally, the resilient support component includes: The support plate is vertically slidably installed on the inner side wall of the insulated box; Medical-grade elastic components are installed on the lower surface of the support plate and press against the bottom wall of the insulated box for positioning.

[0032] By adopting the above technical solution, the medical-grade elastic component is used to drive the support plate to maintain an upward trend, thereby pushing the sample carrier rack to maintain an upward trend, so that the sterile sealing cap is pressed against the box lid for positioning; at the same time, the support plate can be removed to replace the medical-grade elastic component, further ensuring the buffering effect on the sample carrier rack and the test tube body.

[0033] In summary, this application includes at least one of the following beneficial technical effects: 1. During transportation, the temperature control and protection mechanism buffers the sample carrier and test tubes in the horizontal direction; the elastic support component, sterile sealing cap, box lid and insulated box can buffer and position multiple test tubes in the vertical direction, thereby buffering the sample carrier in multiple directions in the horizontal and vertical directions, reducing the risk of multiple test tubes shaking or even being damaged, and improving the reliability of sample testing.

[0034] 2. By combining a ring-shaped temperature control chamber with a temperature protection mechanism, the temperature is set around the test tube body in a more uniform circumferential direction, avoiding the problem of "cold at the bottom and hot at the top, with a large temperature difference" of traditional bottom ice packs, and significantly improving temperature control stability. Moreover, the temperature control chamber wraps multiple test tube bodies inside, so that external heat can be penetrated in all directions, further improving the cooling effect on the test tube bodies, thereby improving the reliability of sample detection.

[0035] 3. The sample is cooled by air before entering the sterile sample chamber. This method allows for direct cooling of the sample and reduces the space required, enabling the cold air to spread and contact the sample more quickly. Furthermore, the three-dimensional cooling from the bottom and all sides improves the cooling and temperature control effect of the sample.

[0036] 4. The activation of the exhaust fan accelerates airflow within the insulated chamber, further enhancing the cooling effect on the samples. Simultaneously, because the diameter of the ventilation holes is smaller than that of the first through-hole, and their number is less than the sum of the first through-hole and the exhaust fan holes, the residence time and uniformity of cold air within the sterile sample chamber are significantly extended. This allows for better and more even cooling of samples within multiple test tubes, greatly improving the cooling effect on the test tubes and ensuring the reliability of sample testing. Attached Figure Description

[0037] Figure 1 This is a three-dimensional structural diagram of a cryogenic storage and transport device; Figure 2 yes Figure 1 A cross-sectional schematic diagram of AA in the middle; Figure 3 yes Figure 1 Cross-sectional schematic diagram of BB; Figure 4 yes Figure 2 Enlarged diagram of section C; Figure 5 yes Figure 2 Enlarged schematic diagram of section D in the middle; Figure 6 yes Figure 2 Enlarged schematic diagram of section E in the middle; Figure 7 This is a partial cross-sectional view of the cryogenic storage and transport device, mainly showing the airflow path.

[0038] Figure reference numerals: 1. Insulated box body; 11. Box lid; 12. Test tube body; 13. Sterile sealing cap; 14. Temperature control chamber; 15. Positioning ring; 16. Receiving slot; 2. Sample carrier rack; 21. Sterile sample chamber; 22. Placement hole; 23. Support block; 24. Support groove; 25. Connector one; 26. Ventilation space; 27. Ventilation hole; 28. First through hole; 29. ​​Air vent; 3. Locking assembly; 31. Locking block; 3 2. Locking rod; 33. Locking plate; 4. Elastic support assembly; 41. Support plate; 42. Medical-grade elastic component; 43. Sterile placement slot; 5. Temperature control protection mechanism; 51. Separating ring; 52. Phase change cold storage cooling component; 53. Connector II; 54. Storage chamber; 55. Low-temperature cold storage chamber; 6. Mounting ring; 61. Sterile drainage chamber; 62. Second through hole; 63. Support ring; 64. Support hole; 65. Mounting hole; 66. Air intake component. Detailed Implementation

[0039] The following provides a further detailed description of this application.

[0040] This application discloses a cryopreservation and transport device for gene detection samples.

[0041] Reference Figures 1-3 A cryogenic transport device for gene testing samples includes an insulated box 1, a lid 11, a sample carrier 2, an elastic support assembly 4, and a temperature control and protection mechanism 5. The sample carrier 2 holds multiple test tubes 12 containing gene testing samples. A sterile sealing cap 13, with a certain degree of elasticity, is inserted and installed at the top of each test tube 12. The sample carrier 2 rests on the elastic support assembly 4, forming an annular temperature-controlled chamber 14 with the inner wall of the insulated box 1. The sterile sealing cap 13 is positioned against the sterile sealing surface inside the lid 11 under the elastic force of the support assembly 4. The temperature control and protection mechanism 5 is located in the temperature-controlled chamber 14 and supports and positions the sample carrier 2, while precisely controlling the temperature of the test tubes 12 to maintain sample stability during transport.

[0042] Reference Figure 1 , Figures 4-6 Both the inner walls of the insulated box body 1 and the box cover 11 are bonded with medical-grade polyurethane insulation layers, and the inner side of the insulation layer is covered with a sterile PE film to prevent the growth of microorganisms. The insulated box body 1 is rectangular or cubic in shape, and a positioning ring 15 is integrally provided on the top of the insulated box body 1 in a ring shape around the insulated box body 1. A concave receiving groove 16 is formed on the lower surface of the box cover 11, so that the box cover 11 has a shell structure. When the receiving groove 16 on the box cover 11 is brought close to the insulated box body 1 with the receiving groove 16 facing down, the positioning ring 15 is inserted into the receiving groove 16, and the box cover 11 is positioned against the top of the insulated box body 1.

[0043] The insulated box 1 is provided with a locking assembly 3 for locking the lid 11. Two sets of locking assemblies 3 are arranged at intervals on both sides of the insulated box 1, and multiple locking assemblies 3 are arranged horizontally at intervals in each set. The locking assembly 3 includes two locking blocks 31, a locking rod 32 and a locking plate 33. The two locking blocks 31 are fixedly installed at intervals on the outer wall of the insulated box 1. The locking rod 32 is rotatably installed on the outer wall of the lid 11 and has a certain elasticity, and is located between the two locking blocks 31. The locking plate 33 is integrally set on the end of the locking rod 32 away from the lid 11. The lid 11 is connected to the insulated box 1. Rotating the locking plate 33 drives the locking rod 32 to rotate, so that the locking rod 32 rotates between the two locking blocks 31, and the locking plate 33 presses against the lower surface of the two locking blocks 31 for positioning, thereby realizing the fixed connection between the lid 11 and the insulated box 1.

[0044] Reference Figures 1-7 The elastic support assembly 4 includes a support plate 41 and medical-grade elastic elements 42. The support plate 41 is vertically slidably installed on the inner side wall of the insulated box 1. The medical-grade elastic elements 42 are springs or sheet springs and are medical-grade. The medical-grade elastic elements 42 are fixedly installed on the lower surface of the support plate 41 and are arranged horizontally at intervals, and are positioned against the inner bottom wall of the insulated box 1. A downwardly recessed sterile placement groove 43 is formed on the upper surface of the support plate 41 and between the multiple medical-grade elastic elements 42. Multiple insertion grooves are opened on the upper surface of the support plate 41 and outside the sterile placement groove 43.

[0045] The sample holder 2 has a sterile sample cavity 21, and multiple placement holes 22 are spaced apart on its upper surface, which communicate with the sterile sample cavity 21 and allow the test tube body 12 to pass through and be placed. Multiple elastic support blocks 23 are spaced apart in the sterile sample cavity 21, which correspond to the multiple placement holes 22. The top of the support block 23 has an arc-shaped support groove 24 for placing and positioning the test tube body 12. The surface of the support groove 24 is coated with a medical sterile coating. Multiple insertion grooves are spaced apart on the upper surface of the sample holder 2 and outside the multiple placement holes 22.

[0046] Multiple connectors 25 are fixedly installed on the lower surface of the sample carrier 2. The sample carrier 2 is placed vertically on the support plate 41, and the multiple connectors 25 are inserted into the multiple connector slots for positioning. At the same time, a ventilation space 26 is formed between the lower surface of the sample carrier 2 and the upper surface of the support plate 41. The temperature control chamber 14 is formed by the cooperation between the outer wall of the sample carrier 2 and the inner wall of the insulation box 1, and is annular. The ventilation space 26 connects the sterile placement slot 43 and the temperature control chamber 14.

[0047] The sample carrier 2 has multiple air inlets 29 on its lower surface that communicate with the sterile sample chamber 21. The air inlets 29 connect the ventilation space 26 and the sterile sample chamber 21. The sample carrier 2 also has multiple first through holes 28 evenly distributed on its outer side wall. The multiple first through holes 28 connect the sterile sample chamber 21 and the temperature control chamber 14. The first through holes 28 and the air inlets 29 have the same diameter, and their diameter is smaller than that of the placement hole 22.

[0048] The temperature control and protection mechanism 5 includes a partition ring 51 and a phase change cold storage cooling component 52. The partition ring 51 is installed on the upper surface of the sample carrier 2 by multiple plug-in parts 53 and is in a vertical position. It is positioned by pressing against the bottom of the receiving groove 16 under the elastic force of the elastic support component 4. The outer wall of the partition ring 51 is the same as the outer wall of the sample carrier 2. Multiple plug-in parts 53 are fixedly installed on the lower surface of the partition ring 51 and are respectively installed in multiple plug-in grooves for positioning. The inner side of the partition ring 51 forms a storage cavity 54, and the outer side cooperates with the inner wall of the insulation box 1 to form a ring-shaped low temperature cold storage cavity 55, so that multiple test tube bodies 12 are located in the storage cavity 54, and the bottom of the low temperature cold storage cavity 55 is connected to the top of the temperature control chamber 14.

[0049] The phase change cold storage cooling element 52 is an ice pack, etc. The phase change cold storage cooling element 52 is filled and placed on the sterile placement tank 43, and the phase change cold storage cooling element 52 is also filled and placed on the temperature control chamber 14, and supports and positions the outer wall of the sample carrier 2. The phase change cold storage cooling element 52 is also filled and placed in the low temperature cold storage chamber 55, and is used to support and position the separator ring 51. A gap is formed between two adjacent phase change cold storage cooling elements 52 located in the sterile placement tank 43, the low temperature cold storage chamber 55 and the temperature control chamber 14 to allow air to pass through, so that air can be cooled by passing through multiple phase change cold storage cooling elements 52.

[0050] A horizontally oriented, annular mounting ring 6 is fixedly installed on the inner wall of the receiving groove 16. The outer wall of the partition ring 51 is slidably installed on the inner wall of the mounting ring 6, and the partition ring 51, the mounting ring 6, and the inner wall of the receiving groove 16 cooperate to form a sterile drainage cavity 61. The partition ring 51 located above the mounting ring 6 has a plurality of second through holes 62 evenly opened. The plurality of second through holes 62 connect the top of the storage cavity 54 and the sterile drainage cavity 61. The diameter of the second through hole 62 is the same as the diameter of the first through hole 28.

[0051] A support ring 63 is fixedly installed on the inner wall of the separator ring 51 and above the second through hole 62. The support ring 63 has multiple support holes 64 that are evenly opened and fitted onto the multiple test tube bodies 12. After the box cover 11 is connected to the heat preservation box 1, the positioning ring 15 is inserted into the receiving groove 16, and the multiple support holes 64 are fitted onto the top of the multiple test tube bodies 12 for positioning.

[0052] The mounting ring 6 has multiple mounting holes 65 spaced apart. An air-guiding component 66 is fixedly installed on the mounting hole 65. The air-guiding component 66 is a fan. A sterile filter membrane is fixedly installed on the upper surface of the air-guiding component 66. Multiple ventilation holes 27 are evenly opened on the upper surface of the sample carrier 2. The multiple ventilation holes 27 connect the storage cavity 54 and the sterile sample cavity 21. The number of ventilation holes 27 is less than the sum of the number of the first through hole 28 and the air-guiding hole 29, and the diameter of the ventilation holes 27 is less than the diameter of the first through hole 28.

[0053] When the fan is started, the air in the storage chamber 54 enters the sterile drainage chamber 61 through the second through hole 62. The air in the sterile drainage chamber 61 is filtered by the sterile filter membrane and then enters the low-temperature cold storage chamber 55. Then the air enters the sterile sample chamber 21 through the temperature control chamber 14 and the first through hole 28 in sequence. Some of the air passing through the temperature control chamber 14 enters the ventilation space 26 and then enters the sterile sample chamber 21 through the ventilation hole 27. Moreover, the air can be cooled by the phase change cold storage cooling element 52 when passing through the low-temperature cold storage chamber, the temperature control chamber 14 and the ventilation space 26. The cooled air enters the sterile sample chamber 21 to cool and control the temperature of the specimen, so that the specimen is in a specified low temperature state, with a temperature of 2-8 degrees Celsius.

[0054] Because the diameter of the ventilation hole 27 is smaller than the diameter of the first through hole 28 and the air vent 29, and the number of ventilation holes 27 is smaller than the sum of the number of the first through hole 28 and the air vent 29, the amount of air input into the sterile sample chamber 21 is greater than the amount of air output through the multiple air vents 29. This prolongs the residence time of cold air in the sterile sample chamber 21 and also allows the air to spread within the sterile sample chamber 21, resulting in a longer cooling time for the samples and more uniform cooling of multiple samples. Then, the air enters the storage chamber 54 through the multiple ventilation holes 27 to achieve air circulation cooling, which can achieve the cooling effect of the samples more quickly, keeping the samples in a suitable temperature environment and improving the reliability of sample detection.

[0055] First, add the gene detection sample into the test tube body 12. Then, insert and install the elastic sterile sealing cap 13 into the test tube body 12, and position the sterile sealing cap 13 against the opening of the test tube body 12. Then, insert and install the test tube body 12 into the placement hole 22, and position the bottom of the test tube body 12 on the support groove 24, so that the sample liquid level in the test tube body 12 is below the placement hole 22.

[0056] An appropriate number of phase change cold storage cooling components 52 are filled and placed on the sterile placement slot 43. The sample carrier 2 is placed vertically on the support plate 41, and multiple connectors 25 are inserted and installed into multiple connector slots for positioning, so that an annular temperature control chamber 14 is formed between the outer wall of the sample carrier 2 and the inner wall of the insulation box 1. An appropriate number of phase change cold storage cooling components 52 are then filled and placed into the temperature control chamber 14. Multiple connectors 53 are then inserted and installed into multiple connector slots, so that the separator ring 51 is installed on the sample carrier 2. The outer wall of the separator ring 51 and the inner wall of the insulation box 1 cooperate to form a low-temperature cold storage chamber 55. An appropriate number of phase change cold storage cooling components 52 are then filled and placed into the low-temperature cold storage chamber 55.

[0057] Positioning ring 15 is inserted into receiving groove 16 for positioning, and box cover 11 is positioned against insulated box 1. Locking component 3 fixes box cover 11 to insulated box 1. Separating ring 51 and sterile sealing cap 13 are both positioned against the bottom of receiving groove 16 under the elastic force of medical-grade elastic element 42. Multiple exhaust fans 66 are activated, and air flow, together with multiple phase change cold storage cooling elements 52, cools the sample, keeping the specimen at the specified low temperature. At the same time, during transportation, medical-grade elastic element 42, support block 23 and sterile sealing cap 13 work together to buffer the test tube body 12, reducing the risk of multiple test tube bodies 12 shaking or even being damaged, thereby improving the reliability of sample detection.

[0058] The working principle of this application embodiment is as follows: First, add the gene detection sample into the test tube body 12, seal the test tube body 12 with a sterile sealing cap 13, then insert the test tube body 12 into the placement hole 22, and position the bottom of the test tube body 12 on the support groove 24, so that the sample liquid level in the test tube body 12 is below the placement hole 22.

[0059] The phase change cold storage cooling element 52 is filled and placed on the sterile placement slot 43. The sample carrier 2 is vertically placed on the support plate 41 and positioned by multiple connectors 25. The phase change cold storage cooling element 52 is then filled and placed into the temperature control chamber 14. The separator ring 51 is installed on the sample carrier 2. An appropriate number of phase change cold storage cooling elements 52 are then filled and placed into the low-temperature cold storage chamber 55. The box cover 11 is connected to the insulated box body 1. The separator ring 51 and the sterile sealing cap 13 are both pressed against the bottom of the receiving slot 16 for positioning under the action of elasticity. Multiple exhaust fans 66 are activated. The air flow, together with the multiple phase change cold storage cooling elements 52, cools the sample, keeping the specimen at the specified low temperature. At the same time, during the transportation process, the medical-grade elastic element 42, the support block 23, and the sterile sealing cap 13 work together to buffer the test tube body 12, reducing the risk of multiple test tube bodies 12 shaking or even being damaged, thereby improving the reliability of sample testing.

[0060] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A cryopreservation and transport device for gene detection samples, characterized in that: Includes an insulated box body (1), a box lid (11), a sample carrier (2), an elastic support assembly (4), and a temperature control and protection mechanism (5); The sample holder (2) is used to place multiple test tube bodies (12) containing gene detection samples. The test tube body (12) is fitted with a sterile sealing cap (13) that is plugged and elastic. The sample carrier (2) is placed on the elastic support component (4) and forms an annular temperature control chamber (14) with the inner wall of the insulated box (1). The sterile sealing cap (13) is pressed against the inner side of the box cover (11) for positioning under the elastic force of the elastic support component (4). The temperature control protection mechanism (5) is located on the temperature control chamber (14) and is used to support and position the sample carrier (2), accurately control the temperature of the test tube body (12) at low temperature, and maintain the stability of the sample during transportation.

2. The cryopreservation and transport device for gene detection samples according to claim 1, characterized in that: The sample holder (2) has a sterile sample cavity (21) and multiple placement holes (22) that communicate with the sterile sample cavity (21) and allow the test tube body (12) to pass through and be placed. Multiple elastic support blocks (23) corresponding to the multiple placement holes (22) are spaced apart in the sterile sample cavity (21). The support blocks (23) have arc-shaped support grooves (24) for placing and positioning the test tube body (12). The surface of the support grooves (24) is coated with a medical sterile coating.

3. The cryopreservation and transport device for gene detection samples according to claim 2, characterized in that: The sample liquid level inside the test tube body (12) is below the placement hole (22). The sample support frame (2) has multiple first through holes (28) connecting the sterile sample chamber (21) and the temperature control chamber (14). The temperature control protection mechanism (5) includes: A separator ring (51) is set on the upper surface of the sample carrier (2) and is positioned against the sterile sealing cap (13) under the elastic force of the elastic support component (4). The inner side of the separator ring (51) forms a storage cavity (54) for placing multiple test tube bodies (12). The separator ring (51) and the inner wall of the heat preservation box (1) cooperate to form a ring-shaped low-temperature cold storage cavity (55) with its bottom communicating with the temperature control chamber (14). The elastic support component (4) has a downwardly recessed sterile placement groove (43) and cooperates with the sample carrier (2) to form a ventilation space (26) communicating with the sterile placement groove (43) and the temperature control chamber (14). The sample carrier (2) has an air vent (29) communicating with the ventilation space (26) and the sterile sample cavity (21) and multiple ventilation holes (27) communicating with the storage cavity (54) and the sterile sample cavity (21). The phase change cold storage cooling component (52) is filled and placed on the sterile placement slot (43), the temperature control chamber (14), and the low temperature cold storage chamber (55), and is used to support and position the sample carrier (2) and the separator ring (51), and to cooperate in the temperature control and cooling treatment of the test tube body (12).

4. The cryopreservation and transport device for gene detection samples according to claim 3, characterized in that: The diameter of the ventilation hole (27) is smaller than the diameter of the first through hole (28), and the number of holes is less than the sum of the number of the first through hole (28) and the number of air vents (29).

5. The cryopreservation and transport device for gene detection samples according to claim 4, characterized in that: The lid (11) has a recessed receiving groove (16). The separator ring (51) and the sterile sealing cap (13) are positioned against the bottom of the receiving groove (16) under the elastic force of the elastic support component (4). The top of the insulated box (1) is provided with a ring-shaped positioning ring (15) that is inserted into the receiving groove (16) for positioning.

6. The cryopreservation and transport device for gene detection samples according to claim 5, characterized in that: An annular mounting ring (6) is provided on the inner wall of the receiving groove (16). The separating ring (51) is slidably mounted on the inner wall of the mounting ring (6) and cooperates with the mounting ring (6) and the inner wall of the receiving groove (16) to form a sterile drainage cavity (61). The mounting ring (6) has multiple mounting holes (65), and the mounting holes (65) are provided with air ducts (66) with sterile filter membranes; the partition ring (51) and above the mounting ring (6) have multiple second through holes (62) connecting the storage cavity (54) and the sterile drainage cavity (61). When the air intake component (66) is started, the gas in the storage chamber (54) is driven to enter the sterile drainage chamber (61) through the second through hole (62), and then sequentially through the low temperature storage chamber (55) and the temperature control chamber (14) before entering the sterile sample chamber (21) to cool and control the temperature of the sample.

7. The cryopreservation and transport device for gene detection samples according to claim 5, characterized in that: It also includes a locking assembly (3) disposed on the insulated box body (1) for locking the box lid (11), the locking assembly (3) comprising: Two locking blocks (31) are spaced apart on the insulation box (1); The locking lever (32) is rotatably mounted on the outer wall of the box cover (11) and has a certain elasticity, and is located between the two locking blocks (31); The locking plate (33) is set on the locking rod (32) and presses against the locking block (31) for positioning.

8. The cryopreservation and transport device for gene detection samples according to claim 3, characterized in that: The lower surface of the sample carrier (2) is positioned on the elastic support assembly (4) by inserting a connector (25); the lower surface of the separator ring (51) is positioned on the upper surface of the sample carrier (2) by inserting a connector (53).

9. The cryopreservation and transport device for gene detection samples according to claim 3, characterized in that: The separating ring (51) is provided with a support ring (63) that is sleeved on the top of multiple test tube bodies (12); the inner walls of both the heat preservation box (1) and the box cover (11) are provided with heat preservation layers.

10. The gene detection sample cryopreservation and transport device according to claim 1, characterized in that: The elastic support component (4) includes: The support plate (41) is vertically slidably installed on the inner wall of the insulation box (1); A medical-grade elastic element (42) is set on the lower surface of the support plate (41) and pressed against the inner bottom wall of the insulated box (1) for positioning.

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