A soothing repair composition and uses thereof

Abstract

The present application provides a soothing repair composition and its application, the soothing repair composition comprises yeast Tricholoma matsutake fermentation product filtrate, dendrobium extract and individual green skin extract. The present application creatively finds that yeast Tricholoma matsutake fermentation product filtrate, dendrobium extract and individual green skin extract have synergistic effect, the three synergistically play the effect of moisturizing, soothing, inhibit inflammation pathway activation, significantly improve the skin barrier repair ability, improve the skin redness, and the skin is more lustrous and bright.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a soothing and repairing composition and its application. Background Technology

[0002] With the increasing use of cosmetics and other chemical products, environmental pollution, faster pace of life, increased stress, and the growing severity of problems such as pollen or food containing allergens, the occurrence of sensitive skin is on the rise. In addition, external stimuli such as improper use of skin care products by some people can easily cause skin allergies.

[0003] Common skin allergic reactions manifest as dryness, flaking, and redness, easily accompanied by sensations such as itching, burning, stinging, and tightness. Sensitive skin is not strictly a skin disease, but rather considered a highly sensitive skin condition. Skin in this state is easily irritated by various physical and chemical factors, causing discomfort and directly impacting quality of life. It is generally believed that sensitive skin is the result of the combined effects of internal and external factors, with a complex and diverse range of triggering factors.

[0004] Currently, the main ingredients for immediate relief of skin problems such as allergies are corticosteroids. These drugs soothe allergic reactions by suppressing the body's immune response. However, the use of corticosteroids not only suppresses the abnormal immune response that causes allergies, but also suppresses the normal immune response, reduces the resistance of skin cells, and thus induces adverse reactions such as non-allergic dermatitis, skin tumors, melanin deposition, and abnormal skin growth. Long-term use can also lead to drug dependence.

[0005] Therefore, there is an urgent need to develop a skin care product that moisturizes, soothes, and repairs the skin barrier. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the present invention aims to provide a soothing and repairing composition and its application. The present invention creatively discovers that yeast and matsutake fermentation product filtrate, dendrobium extract and citrus peel extract have a synergistic effect, and the three work together to moisturize, soothe and repair the skin barrier.

[0007] To achieve this objective, the present invention adopts the following technical solution: In a first aspect, the present invention provides a soothing and repairing composition comprising yeast and matsutake fermentation product filtrate, dendrobium extract and citrus peel extract.

[0008] The yeast-matsutake fermentation product filtrate is rich in polysaccharides, terpenes, polyphenols, and other active ingredients. It can promote cell proliferation, migration, and differentiation to form new keratinocytes, thus repairing the skin barrier. It can also regulate the sensitive switch NF-κB, inhibiting inflammatory responses by suppressing inflammatory factors such as TNF-α and IL-6, achieving a soothing effect. Dendrobium extract contains polysaccharides, flavonoids, phenols, vitamins, and amino acids. It upregulates the expression of AQP3 and FLG genes, regulates skin moisturizing-related genes, and promotes skin hydration and barrier repair. Green tangerine peel extract contains flavonoids and alkaloids, inhibiting the expression and activation of TRPV1 in keratinocytes, relieving stinging and redness in sensitive skin.

[0009] This invention creatively discovers that yeast-matsutake fermentation product filtrate, dendrobium extract, and citrus peel extract have a synergistic effect, and the three work together to moisturize, soothe, and repair the skin barrier.

[0010] Preferably, the soothing and repairing composition comprises, by weight, 1-6 parts of yeast and matsutake fermentation product filtrate, 0.1-5 parts of Dendrobium extract, and 0.1-2 parts of Citrus reticulata tangerine peel extract.

[0011] The weight fractions of the yeast-matsutake fermentation product filtrate can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, or 6 parts, etc.

[0012] The weight parts of the Dendrobium extract can be 0.1 parts, 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, or 5 parts, etc.

[0013] The weight percentage of the green tangerine peel extract can be 0.1 parts, 0.2 parts, 0.5 parts, 0.7 parts, 1 part, 1.3 parts, 1.5 parts, 1.8 parts, or 2 parts, etc.

[0014] Preferably, the yeast-matsutake fermentation product filtrate is prepared by a method comprising the following steps: Matsutake mushroom powder, brewer's yeast seed liquid, and culture medium are mixed and fermented to obtain fermentation broth; the fermentation broth is centrifuged and filtered to obtain the yeast-matsutake mushroom fermentation product filtrate.

[0015] Preferably, the ratio of matsutake powder to culture medium is 1 g:(100~1000) mL.

[0016] The specific point values ​​in (100~1000) can be 100, 200, 300, 400, 500, 600, 700, 800, 900 or 1000, etc.

[0017] Preferably, the ratio of the brewer's yeast seed liquid to the culture medium is 1 g:(10~100) mL.

[0018] The specific point values ​​in (10~100) can be 10, 20, 30, 40, 50, 60, 70, 80, 90 or 100, etc.

[0019] Preferably, the OD of the brewer's yeast seed liquid 600 The value can be 2 to 5, for example, it can be 2, 2.5, 3, 3.5, 4, 4.5 or 5, etc.

[0020] Preferably, the fermentation temperature is 28~32℃, for example, 28℃, 28.5℃, 29℃, 29.5℃, 30℃, 30.5℃, 31℃, 31.5℃ or 32℃; the fermentation time is 4~36 h, for example, 4 h, 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, 15 h, 20 h, 25 h, 30 h, 35 h or 36 h.

[0021] Preferably, the centrifugation speed is 5000~15000 rpm, for example, 5000 rpm, 6000 rpm, 7000 rpm, 8000 rpm, 9000 rpm, 10000 rpm, 11000 rpm, 12000 rpm, 13000 rpm, 14000 rpm or 15000 rpm; the time is 10~40 min, for example, 10 min, 15 min, 20 min, 25 min, 30 min, 35 min or 40 min.

[0022] Preferably, the pore size of the filter membrane is 0.1~0.45 μm, for example, it can be 0.1 μm, 0.15 μm, 0.2 μm, 0.25 μm, 0.3 μm, 0.35 μm, 0.4 μm or 0.45 μm, etc.

[0023] Preferably, the Dendrobium extract is prepared by a method comprising the following steps: Dendrobium was extracted with water, and the solid and liquid were separated. The liquid was collected to obtain Dendrobium extract.

[0024] Dendrobium extracts prepared by conventional extraction methods are all applicable to this invention, and are not limited to the preparation methods described above.

[0025] Preferably, the mass ratio of Dendrobium to water is 1:(30~40).

[0026] The specific point values ​​in (30~40) can be 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 or 40, etc.

[0027] Preferably, the extraction temperature is 70~90℃, for example, 70℃, 72℃, 75℃, 77℃, 80℃, 83℃, 85℃, 88℃ or 90℃; the extraction time is 2~4 h, for example, 2 h, 2.2 h, 2.5 h, 2.7 h, 3 h, 3.3 h, 3.5 h, 3.8 h or 4 h.

[0028] Preferably, the green tangerine peel extract is prepared by a method comprising the following steps: The green tangerine peel was extracted with an acidic aqueous solution, and the solid and liquid were separated and the liquid was collected. The solid was then extracted with an alcoholic aqueous solution, and the solid and liquid were separated and the liquid was collected. The liquids collected in both cases were mixed to obtain the green tangerine peel extract.

[0029] Preferably, the acid in the aqueous solution includes any one or a combination of at least two of formic acid, acetic acid, or hydrochloric acid.

[0030] Preferably, the pH value of the acidic aqueous solution is 2 to 5, for example, it can be 2, 2.2, 2.5, 2.7, 3, 3.3, 3.5, 3.8, 4, 4.5 or 5.

[0031] Preferably, the mass ratio of the green tangerine peel to the acidic aqueous solution is 1:(10~20).

[0032] The specific point values ​​in (10~20) can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20, etc.

[0033] Preferably, the alcohol in the aqueous alcohol solution includes methanol and / or ethanol.

[0034] Preferably, the concentration of the alcohol-water solution is 50-95 vol%, for example, it can be 50 vol%, 55 vol%, 60 vol%, 65 vol%, 70 vol%, 75 vol%, 80 vol%, 85 vol%, 90 vol%, or 95 vol%.

[0035] Preferably, the mass ratio of the green tangerine peel to the alcohol-water solution is 1:(5~20).

[0036] The specific point values ​​in (5~20) can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20, etc.

[0037] Preferably, the extraction temperature is 30~60℃, for example, 30℃, 35℃, 40℃, 45℃, 50℃, 55℃ or 60℃; the time is 0.5~5 h, for example, 0.5 h, 1 h, 1.5 h, 2 h, 2.5 h, 3 h, 3.5 h, 4 h, 4.5 h or 5 h.

[0038] In a second aspect, the present invention provides the use of the soothing and repairing composition as described in the first aspect in the preparation of cosmetics.

[0039] Preferably, the cosmetic includes any one of toner, lotion, cream, essence water, serum, facial cleanser, spray, or mask.

[0040] Preferably, the amount of the soothing and repairing composition added to the cosmetic is 1.2 to 13 wt%, for example, it can be 1.2 wt%, 1.5 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%, 11 wt%, 12 wt%, or 13 wt%.

[0041] Preferably, the cosmetic also includes any one or a combination of at least two of the following: skin conditioning agents, moisturizers, thickeners, solubilizers, pH adjusters, chelating agents, fragrances, preservatives, emollients, emulsifiers, or solvents.

[0042] Thirdly, the present invention provides a soothing and repairing facial mask, wherein the soothing and repairing facial mask comprises, by weight, 1.2 to 13 parts of the soothing and repairing composition as described in the first aspect, 0.15 to 1.5 parts of a skin conditioning agent, 0.02 to 15 parts of a moisturizer, 0.02 to 1 part of a thickener, 0.005 to 0.3 parts of a pH adjuster, 0.01 to 0.5 parts of a chelating agent, and 60 to 99 parts of a solvent.

[0043] The soothing and repairing composition can be in the following weight proportions: 1.2 parts, 1.5 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, or 13 parts.

[0044] The skin conditioning agent can be present in weight parts of 0.15, 0.2, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 parts, etc.

[0045] The weight parts of the humectant can be 0.02 parts, 0.05 parts, 0.1 parts, 0.5 parts, 1 part, 2 parts, 5 parts, 7 parts, 10 parts, 13 parts or 15 parts, etc.

[0046] The thickener can be present in parts by weight of 0.02, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, or 1 part, etc.

[0047] The pH adjuster can be present in weight parts of 0.005, 0.01, 0.05, 0.1, 0.15, 0.2, 0.25, or 0.3, etc.

[0048] The weight parts of the chelating agent can be 0.01 parts, 0.05 parts, 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, 0.45 parts, or 0.5 parts, etc.

[0049] The solvent may be present in the following weight percentages: 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 75, 80, 85, 90, 95, 96, 97, 98, or 99.

[0050] The facial mask provided by this invention has excellent moisturizing and soothing effects and can repair the skin barrier.

[0051] Preferably, the skin conditioning agent comprises any one or a combination of at least two of 3-O-ethyl ascorbic acid, ceramide NS, or β-glucan.

[0052] Preferably, the skin conditioning agent comprises 3-O-ethyl ascorbic acid, ceramide NS, and β-glucan.

[0053] 3-O-ethyl ascorbic acid, ceramide NS and β-glucan have a synergistic effect, and the combination of the three helps to enhance moisturizing, soothing and skin barrier repair effects.

[0054] Preferably, the mass ratio of 3-O-ethyl ascorbic acid, ceramide NS and β-glucan is (0.05~0.5):(0.0005~0.1):(0.1~1).

[0055] The specific point values ​​in (0.05~0.5) can be 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45 or 0.5, etc.

[0056] The specific point values ​​in (0.0005~0.1) can be 0.0005, 0.0006, 0.0007, 0.0008, 0.0009, 0.001, 0.01 or 0.1, etc.

[0057] The specific point values ​​in (0.1~1) can be 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 or 1, etc.

[0058] Preferably, the moisturizer comprises any one or a combination of at least two of the following: glycerin, butylene glycol, panthenol, dipropylene glycol, 1,3-propanediol, trehalose, betaine, glyceryl glucoside, or sodium hyaluronate.

[0059] Preferably, the thickener comprises any one or a combination of at least two of the following: carbomer, acrylate / C10-30 alkanol acrylate crosspolymer, sodium polyacrylate, ammonium acryloyldimethyl taurate / VP copolymer, polyacrylate crosspolymer-6, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, sodium polyacryloyldimethyl taurate, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, xanthan gum, sclerotium gum, or agarose.

[0060] Preferably, the pH adjuster comprises any one or a combination of at least two of citric acid, sodium citrate, lactic acid, arginine, triethanolamine, aminomethylpropanol, sodium hydroxide, or potassium hydroxide.

[0061] Preferably, the chelating agent includes any one or a combination of at least two of disodium EDTA, trisodium EDTA, or tetrasodium EDTA.

[0062] Preferably, the solvent includes any one or a combination of at least two of the following: deionized water, aloe vera leaf water, birch sap, white chrysanthemum flower water, rose water, witch hazel water, or calendula water.

[0063] Fourthly, the present invention provides a method for preparing a soothing and repairing facial mask as described in the third aspect, the method comprising: Moisturizer, thickener, pH adjuster, chelating agent and solvent are mixed, and then skin conditioning agent and soothing and repairing composition as described in the first aspect are added and stirred to obtain mask essence; the mask base cloth is soaked in mask essence to obtain the soothing and repairing mask.

[0064] Preferably, the mixing temperature is 80~85℃, for example, it can be 80℃, 80.5℃, 81℃, 81.5℃, 82℃, 82.5℃, 83℃, 83.5℃, 84℃, 84.5℃ or 85℃, etc.

[0065] Preferably, the mixing speed is 10~40 rpm, for example, it can be 10 rpm, 15 rpm, 20 rpm, 25 rpm, 30 rpm, 35 rpm or 40 rpm, etc.

[0066] Preferably, the mixing time is 20 to 30 minutes, for example, 20 minutes, 21 minutes, 22 minutes, 23 minutes, 24 minutes, 25 minutes, 26 minutes, 27 minutes, 28 minutes, 29 minutes, or 30 minutes.

[0067] Preferably, the stirring temperature is 35~40℃, for example, it can be 35℃, 35.5℃, 36℃, 36.5℃, 37℃, 37.5℃, 38℃, 38.5℃, 39℃, 39.5℃ or 40℃, etc.

[0068] Preferably, the stirring speed is 10~40 rpm, for example, it can be 10 rpm, 15 rpm, 20 rpm, 25 rpm, 30 rpm, 35 rpm or 40 rpm.

[0069] Preferably, the stirring time is 20 to 30 minutes, for example, 20 minutes, 21 minutes, 22 minutes, 23 minutes, 24 minutes, 25 minutes, 26 minutes, 27 minutes, 28 minutes, 29 minutes, or 30 minutes.

[0070] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0071] Compared with the prior art, the present invention has the following beneficial effects: This invention creatively discovers that yeast and matsutake fermentation product filtrate, dendrobium extract, and citrus peel extract have a synergistic effect. The three work together to moisturize and soothe, inhibit the activation of inflammatory pathways, significantly improve the skin barrier repair ability, reduce skin redness, and make the skin more radiant and translucent. Detailed Implementation

[0072] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0073] Where specific techniques or conditions are not specified in the examples, they shall be performed in accordance with the techniques or conditions described in the literature in this field, or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased through legitimate channels.

[0074] The sources of materials used in the following specific embodiments are as follows: brewer's yeast is brewer's yeast Saccharomyces cerevisiae CICC 1001 was purchased from the China Industrial Microbial Culture Collection Center.

[0075] Mix and dissolve 20 g / L peptone, 15 g / L yeast extract, 20 g / L glucose, 4 g / L sodium dihydrogen phosphate, 3 g / L magnesium sulfate, 20 mg / L thioamine and water, and sterilize at 121℃ for 20 min to prepare Saccharomyces cerevisiae culture medium.

[0076] Preparation Example 1 This preparation example provides a yeast-matsutake mushroom fermentation product filtrate, the preparation method of which includes: Mix 1 g of matsutake mushroom powder with 500 mL of Saccharomyces cerevisiae culture medium, then add 10 g of Saccharomyces cerevisiae seed culture (OD200). 600 The value is 3), fermented at 30℃ for 20 h to obtain fermentation broth; the fermentation broth is centrifuged at 10000 rpm for 20 min, the supernatant is taken and filtered through a 0.22 μm membrane to obtain the final product.

[0077] Preparation Example 2 This preparation example provides a yeast-matsutake mushroom fermentation product filtrate, the preparation method of which includes: Mix 1 g of matsutake mushroom powder with 100 mL of Saccharomyces cerevisiae culture medium, then add 1 g of Saccharomyces cerevisiae seed culture (OD200). 600 With a value of 2), ferment at 32℃ for 10 h to obtain fermentation broth; centrifuge the fermentation broth at 5000 rpm for 40 min, take the supernatant, and filter it through a 0.22 μm membrane to obtain the final product.

[0078] Preparation Example 3 This preparation example provides a yeast-matsutake mushroom fermentation product filtrate, the preparation method of which includes: Mix 1 g of matsutake mushroom powder with 1000 mL of Saccharomyces cerevisiae culture medium, then add 10 g of Saccharomyces cerevisiae seed culture (OD200). 600 The fermentation broth was obtained by fermenting at 28℃ for 36 h with a value of 5. The fermentation broth was centrifuged at 15000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm membrane.

[0079] Preparation Example 4 This preparation example provides a Dendrobium extract, the preparation method of which includes: Mix 10 g of Dendrobium officinale powder with 350 g of water, extract at 80℃ for 3 h, filter, collect the filtrate, concentrate and dry to obtain the final product.

[0080] Preparation Example 5 This preparation example provides a Dendrobium extract, the preparation method of which includes: Mix 10 g of Dendrobium officinale powder with 300 g of water, extract at 90℃ for 2 h, filter, collect the filtrate, concentrate and dry to obtain the final product.

[0081] Preparation Example 6 This preparation example provides a Dendrobium extract, the preparation method of which includes: Mix 10 g of Dendrobium officinale powder with 400 g of water, extract at 70℃ for 4 h, filter, collect the filtrate, concentrate and dry to obtain the final product.

[0082] Preparation Example 7 This preparation example provides a green tangerine peel extract, the preparation method of which includes: Mix 10 g of green tangerine peel powder with 150 g of hydrochloric acid aqueous solution (pH value 3), extract at 45℃ for 3 h, filter, and collect the filtrate; add 100 g of 70 vol% ethanol aqueous solution to the remaining filter residue, extract at 50℃ for 2 h, filter, and collect the filtrate; mix the two collected filtrates, concentrate and dry to obtain the final product.

[0083] Preparation Example 8 This preparation example provides a green tangerine peel extract, the preparation method of which includes: Mix 10 g of green tangerine peel powder with 200 g of formic acid aqueous solution (pH value 2), extract at 60℃ for 1 h, filter, and collect the filtrate; add 200 g of 50 vol% methanol aqueous solution to the remaining filter residue, extract at 30℃ for 5 h, filter, and collect the filtrate; mix the two collected filtrates, concentrate and dry to obtain the final product.

[0084] Preparation Example 9 This preparation example provides a green tangerine peel extract, the preparation method of which includes: Mix 10 g of green tangerine peel powder with 100 g of acetic acid aqueous solution (pH 5), extract at 30℃ for 5 h, filter, and collect the filtrate; add 50 g of 95 vol% ethanol aqueous solution to the remaining filter residue, extract at 60℃ for 1 h, filter, and collect the filtrate; mix the two collected filtrates, concentrate and dry to obtain the final product.

[0085] Example 1 This embodiment provides a composition comprising, by weight parts: Three portions of yeast-matsutake fermentation product filtrate from Preparation Example 1, two portions of Dendrobium extract from Preparation Example 4, and one portion of tangerine peel extract from Preparation Example 7.

[0086] Example 2 This embodiment provides a composition comprising, by weight parts: 1 part of yeast-matsutake fermentation product filtrate provided in Preparation Example 2, 0.1 part of Dendrobium extract provided in Preparation Example 5, and 0.1 part of citrus tangerine peel extract provided in Preparation Example 8.

[0087] Example 3 This embodiment provides a composition comprising, by weight parts: Six portions of yeast-matsutake fermentation product filtrate from Preparation Example 3, five portions of Dendrobium extract from Preparation Example 6, and two portions of tangerine peel extract from Preparation Example 9.

[0088] Comparative Example 1 This comparative example provides a composition, and the only difference from the other examples 1 is that: no yeast matsutake fermentation product filtrate was added, and its reduced amount was proportionally allocated to the dendrobium extract and the green tangerine peel extract.

[0089] Comparative Example 2 This comparative example provides a composition that differs from the rest of Example 1 only in that: no Dendrobium extract was added, and its reduced amount was proportionally allocated to the yeast matsutake fermentation product filtrate and the green tangerine peel extract.

[0090] Comparative Example 3 This comparative example provides a composition that differs from the rest of Example 1 only in that: no green tangerine peel extract was added, and its reduced amount was proportionally allocated to the yeast matsutake fermentation product filtrate and dendrobium extract.

[0091] Application Example 1 This application example provides a face mask that comprises the following components in parts by weight: Its preparation methods include: Moisturizer, thickener, pH adjuster, chelating agent and solvent are mixed and heated to 82°C and stirred at 30 rpm for 25 min; then cooled to 37°C, skin conditioning agent and composition are added, and stirred at 30 rpm for 25 min to obtain mask essence; the mask base cloth is soaked in mask essence to obtain the final product.

[0092] Application Example 2 This application example provides a face mask that comprises the following components in parts by weight: Its preparation methods include: Moisturizer, thickener, pH adjuster, chelating agent and solvent are mixed and heated to 80℃ and stirred at 40 rpm for 20 min; cooled to 35℃, skin conditioning agent and composition are added, and stirred at 40 rpm for 20 min to obtain mask essence; the mask base cloth is soaked in mask essence to obtain the mask.

[0093] Application Example 3 This application example provides a face mask that comprises the following components in parts by weight: Its preparation methods include: Moisturizer, thickener, pH adjuster, chelating agent and solvent are mixed and heated to 85°C and stirred at 10 rpm for 30 min; then cooled to 40°C, skin conditioning agent and composition are added, and stirred at 10 rpm for 30 min to obtain mask essence; the mask base cloth is soaked in mask essence to obtain the final product.

[0094] Application Example 4 This application example provides a face mask that differs from Application Example 1 only in that: 3-O-ethyl ascorbic acid is not added, and its reduced amount is proportionally allocated to ceramide NS and β-glucan, while the other raw materials and steps remain unchanged.

[0095] Application Example 5 This application example provides a face mask that differs from Application Example 1 only in that: ceramide NS is not added, and its reduced amount is proportionally allocated to 3-O-ethyl ascorbic acid and β-glucan, while the other raw materials and steps remain unchanged.

[0096] Application Example 6 This application example provides a face mask that differs from Application Example 1 only in that: β-glucan is not added, and its reduced amount is proportionally allocated to 3-O-ethyl ascorbic acid and ceramide NS, while the other raw materials and steps remain unchanged.

[0097] Application Example 7 This application example provides a face mask that differs from Application Example 1 only in that 3-O-ethyl ascorbic acid is replaced with an equal amount of ascorbic acid, while the other raw materials and steps remain unchanged.

[0098] Compare and contrast examples 1-3 This comparative application example provides a face mask, which differs from Application Example 1 only in that the "composition provided in Example 1" is replaced with an equal amount of the "composition provided in Comparative Examples 1 to 3", while the other raw materials and steps remain unchanged.

[0099] Test Example 1 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable free radical. Its ethanol solution is deep purple and exhibits strong absorption at a wavelength of 517 nm. In the presence of a free radical scavenger, the light absorption of the DPPH ethanol solution decreases due to the pairing of unpaired electrons with the DPPH. The degree of fading of the DPPH ethanol solution is linearly related to the number of electrons it accepts, thus allowing evaluation of the test sample's ability to scavenge free radicals, i.e., its antioxidant capacity.

[0100] The compositions provided in Examples 1-3 and Comparative Examples 1-3 were prepared into 0.1 mg / mL test sample solutions by adding water. 2 mL of the test sample solution and 2 mL of 2×10⁻⁶ ppm solution were added to a 10 mL sealed test tube. -4Mix mM DPPH ethanol solution thoroughly, let stand at room temperature in the dark for 30 min, and measure the absorbance A1 at 517 nm using a UV-Vis spectrophotometer. Use the same volume of water instead of the test sample solution as a negative control and measure the absorbance A2. Use the same volume of anhydrous ethanol instead of the DPPH ethanol solution as a blank control and measure the absorbance A3. Design 3 replicates for each group, calculate the average value, and calculate the DPPH scavenging rate: DPPH scavenging rate (%) = [1 - (A1 - A3) / A2] × 100%.

[0101] As shown in Table 1, it can be seen from Examples 1-3 and Comparative Examples 1-3 that the yeast-matsutake fermentation product filtrate, Dendrobium extract and Citrus reticulata peel extract have a synergistic effect, have excellent DPPH scavenging effect, synergistic antioxidant effect, inhibit the activation of inflammatory pathways, and create a homeostatic environment free from oxidative stress for cells.

[0102] Table 1 Test Example 2 The test substances were the facial mask essences from Application Examples 1-7 and Comparative Application Examples 1-3, with deionized water as a negative control. A total of 30 subjects were included (10 males and 20 females), aged 27-58 years, with a mean age of 40.0 ± 8.9 years, meeting the subject voluntary inclusion criteria. Using qualified patch testing equipment, approximately 0.020-0.025 mL of the test substance was placed in the patch tester using a closed patch test method, and then applied to the back of the subject with hypoallergenic adhesive tape. The test substance was removed after 24 hours, and skin reactions were observed at 0.5, 24, and 48 hours after removal. The test results were recorded according to the skin reaction grading standards for the closed patch test method shown in Table 2. The results showed that all test samples exhibited a "score grade 0, negative reaction" at all test time points.

[0103] Table 2 Test Example 3 The subjects were Asian adult women aged 18-50 with sensitive skin. They were randomly assigned to two groups of 10 each to use the masks described in Examples 1-7 and the control group described in Examples 1-3. No other functional products were used during the test. The following parameters were measured using an instrument probe: skin stratum corneum moisture content (higher values ​​indicate higher skin moisture content), skin transepidermal water loss (lower values ​​indicate better skin barrier function), skin hemoglobin content (higher values ​​indicate a higher proportion of skin redness), and skin radiance (higher values ​​indicate better skin radiance). The rate of change of each indicator after 28 days of continuous product use compared to before use was calculated.

[0104] As shown in Table 3, Application Examples 1-3 and Comparative Application Examples 1-3 demonstrate that the yeast-matsutake fermentation product filtrate, Dendrobium officinale extract, and Citrus reticulata peel extract have a synergistic effect, significantly enhancing the skin barrier repair ability, improving skin redness, and increasing radiance. Application Examples 4-6 demonstrate that 3-O-ethyl ascorbic acid, ceramide NS, and β-glucan have a synergistic effect; the combination of these three ingredients helps enhance anti-inflammatory and soothing effects, and improves the skin barrier repair ability.

[0105] Table 3 This invention illustrates a soothing and repairing composition and its application through the above embodiments. However, this invention is not limited to the above embodiments, meaning that this invention does not necessarily rely on the above embodiments for implementation. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of the raw materials in the product, addition of auxiliary components, and selection of specific methods, all fall within the protection and disclosure scope of this invention.

[0106] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0107] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A soothing and repairing composition, characterized in that, The soothing and repairing composition includes yeast-matsutake fermentation product filtrate, dendrobium extract, and citrus tangerine peel extract.

2. The soothing and repairing composition according to claim 1, characterized in that, The soothing and repairing composition comprises, by weight, 1-6 parts of yeast and matsutake fermentation product filtrate, 0.1-5 parts of dendrobium extract, and 0.1-2 parts of green tangerine peel extract.

3. The soothing and repairing composition according to claim 1 or 2, characterized in that, The yeast-matsutake mushroom fermentation product filtrate was prepared by a method comprising the following steps: Matsutake mushroom powder, brewer's yeast seed liquid, and culture medium were mixed and fermented to obtain fermentation broth; the fermentation broth was centrifuged and filtered to obtain the yeast-matsutake mushroom fermentation product filtrate. Preferably, the ratio of matsutake mushroom powder to culture medium is 1 g:(100~1000) mL; Preferably, the ratio of the brewer's yeast seed liquid to the culture medium is 1 g:(10~100) mL; Preferably, the OD of the brewer's yeast seed liquid 600 Values ​​range from 2 to 5; Preferably, the fermentation temperature is 28~32℃ and the time is 4~36 h; Preferably, the centrifugation speed is 5000~15000 rpm and the time is 10~40 min; Preferably, the pore size of the filter membrane is 0.1~0.45 μm.

4. The use of the soothing and repairing composition according to any one of claims 1 to 3 in the preparation of cosmetics.

5. The application according to claim 4, characterized in that, The cosmetics include any one of the following: toner, lotion, face cream, essence water, serum, facial cleanser, spray, or face mask; Preferably, the amount of the soothing and repairing composition added to the cosmetic is 1.2~13 wt%; Preferably, the cosmetic also includes any one or a combination of at least two of the following: skin conditioning agents, moisturizers, thickeners, solubilizers, pH adjusters, chelating agents, fragrances, preservatives, emollients, emulsifiers, or solvents.

6. A soothing and repairing facial mask, characterized in that, The soothing and repairing mask comprises, by weight, 1.2 to 13 parts of the soothing and repairing composition as described in any one of claims 1 to 3, 0.15 to 1.5 parts of a skin conditioning agent, 0.02 to 15 parts of a moisturizer, 0.02 to 1 part of a thickener, 0.005 to 0.3 parts of a pH adjuster, 0.01 to 0.5 parts of a chelating agent, and 60 to 99 parts of a solvent.

7. The soothing and repairing facial mask according to claim 6, characterized in that, The skin conditioning agent includes any one or a combination of at least two of 3-O-ethyl ascorbic acid, ceramide NS, or β-glucan; Preferably, the skin conditioning agent comprises 3-O-ethyl ascorbic acid, ceramide NS, and β-glucan; Preferably, the mass ratio of 3-O-ethyl ascorbic acid, ceramide NS and β-glucan is (0.05~0.5):(0.0005~0.1):(0.1~1).

8. The soothing and repairing facial mask according to claim 6 or 7, characterized in that, The moisturizer includes any one or a combination of at least two of the following: glycerin, butylene glycol, panthenol, dipropylene glycol, 1,3-propanediol, trehalose, betaine, glyceryl glucoside, or sodium hyaluronate. Preferably, the thickener comprises any one or a combination of at least two of the following: carbomer, acrylate / C10-30 alkanol acrylate crosspolymer, sodium polyacrylate, ammonium acryloyl dimethyl taurate / VP copolymer, polyacrylate crosspolymer-6, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, sodium polyacryloyl dimethyl taurate, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, xanthan gum, sclerotium gum, or agarose; Preferably, the pH adjuster comprises any one or a combination of at least two of citric acid, sodium citrate, lactic acid, arginine, triethanolamine, aminomethylpropanol, sodium hydroxide, or potassium hydroxide; Preferably, the chelating agent comprises any one or a combination of at least two of disodium EDTA, trisodium EDTA, or tetrasodium EDTA; Preferably, the solvent includes any one or a combination of at least two of the following: deionized water, aloe vera leaf water, birch sap, white chrysanthemum flower water, rose water, witch hazel water, or calendula water.

9. The method for preparing the soothing and repairing facial mask according to any one of claims 6 to 8, characterized in that, The preparation method includes: A moisturizer, thickener, pH adjuster, chelating agent and solvent are mixed, and then a skin conditioning agent and the soothing and repairing composition according to any one of claims 1 to 3 are added and stirred to obtain a mask essence; the mask base cloth is soaked in the mask essence to obtain the soothing and repairing mask.

10. The preparation method according to claim 9, characterized in that, The mixing temperature is 80~85℃; Preferably, the mixing speed is 10~40 rpm; Preferably, the mixing time is 20-30 minutes; Preferably, the stirring temperature is 35~40℃; Preferably, the stirring speed is 10~40 rpm; Preferably, the stirring time is 20-30 minutes.

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