A red fade repair plant extract composition, and a preparation method and application thereof

Abstract

The application belongs to the technical field of cosmetics, and particularly relates to a redness-reducing and repairing plant extract composition as well as a preparation method and application thereof. The preparation method of the plant extract composition comprises the following steps: (1) freeze-grinding dried leaves of Rehmannia glutinosa, rosemary, oat kernels, peppermint and radix Stephaniae tetrandrae after soaking in warm water to obtain plant raw material powder; (2) adding the obtained plant raw material powder into deionized water, then adding hydroxypropyl cyclodextrin and glycerophosphoinositol choline salt as auxiliary extractants, stirring and extracting at a temperature of 20-50 DEG C, filtering and sterilizing after concentration to obtain an extract; and (3) diluting the obtained extract with a mixed solvent of butanediol and hexanediol, then adding a preservative and uniformly mixing to obtain the redness-reducing and repairing plant extract composition. The hydroxypropyl cyclodextrin and glycerophosphoinositol choline salt are used for low-temperature auxiliary extraction of the plant raw material, which can significantly improve the soothing, redness-reducing and repairing effects of the obtained plant extract composition.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a redness-reducing and repairing plant extract composition, its preparation method, and its application. Background Technology

[0002] Plant extracts are widely used in cosmetics due to their antioxidant, soothing, repairing, moisturizing, whitening, and antibacterial properties, as well as their gentler and safer effects compared to chemically synthesized ingredients. For example, patent document CN 120189363 A discloses a soothing composition comprising ectoine, centella asiatica extract, symcalmin, purslane extract, and panthenol. This composition exhibits significant synergistic effects in moisturizing, repairing, and soothing. Patent document CN 116850115 A discloses a composition with soothing effects on sensitive skin, consisting of the following components in weight percentages: 10-12% plant extract, 2-3% skin repair factor, 10% jasmine hydrosol, 6-7% moisturizer, and the balance being water. This composition has significant soothing and moisturizing effects.

[0003] However, the efficacy of plant extracts is greatly affected by the extraction process. Currently, the main extraction method for plant extracts is solvent extraction (water, organic solvents), which has the advantages of simple process and low cost. However, it suffers from poor selectivity and low extraction efficiency of active ingredients. On the other hand, methods such as ionic liquid-assisted extraction and supercritical fluid extraction have the problems of difficult product separation, high process control difficulty, and high cost. Summary of the Invention

[0004] In view of the shortcomings and deficiencies of the existing technology, the primary objective of this invention is to provide a method for preparing a redness-reducing and repairing plant extract composition.

[0005] Another object of the present invention is to provide a redness-reducing and repairing plant extract composition prepared by the above method.

[0006] Another object of the present invention is to provide the application of the above-mentioned redness-reducing and repairing plant extract composition in cosmetics.

[0007] The objective of this invention is achieved through the following technical solution:

[0008] A method for preparing a redness-reducing and repairing plant extract composition includes the following preparation steps:

[0009] (1) The dried Centella asiatica leaves, rosemary, oat kernels, peppermint and Stephania tetrandra raw materials were soaked in warm water and then frozen and ground to obtain plant raw material powder;

[0010] (2) Add the obtained plant raw material powder to deionized water, then add hydroxypropyl cyclodextrin and glycerophosphate inositol choline salt as auxiliary extraction agents, stir and extract at 20~50℃, concentrate and filter to remove bacteria to obtain the extract;

[0011] (3) The obtained extract was diluted with a mixed solvent of butanediol and hexanediol, and then a preservative was added and mixed well to obtain a redness-reducing plant extract composition.

[0012] Further, the mass ratio of the Centella asiatica leaves, rosemary, oat kernels, peppermint and Stephania tetrandra raw materials in step (1) is as follows: Centella asiatica leaves 30-60 parts, rosemary 5-10 parts, oat kernels 2-5 parts, peppermint 2-5 parts, Stephania tetrandra 0.5-2 parts.

[0013] This invention uses Centella asiatica leaves as the main extraction raw material, which contains rich anti-inflammatory and repairing active ingredients. Combined with the soothing active ingredients of rosemary and oat kernels, and the anti-inflammatory active ingredients of peppermint and Stephania tetrandra, the soothing, redness-reducing and repairing effects of the resulting extract composition are significantly improved.

[0014] Furthermore, the warm water soaking in step (1) is preferably done in 50-60℃ warm water for 4-8 hours.

[0015] Further, the cryogenic grinding in step (1) is preferably carried out at -50 to -80°C until the powder particle size is 50 to 500 μm.

[0016] This invention uses a method of soaking in warm water followed by freeze grinding to effectively destroy the cell membranes and cell walls of plant raw materials, thereby ensuring the extraction effect of subsequent low-temperature extraction.

[0017] Furthermore, in step (2), the mass ratio of the plant raw material powder to the deionized water is 1:6~12.

[0018] Further, in step (2), the amount of hydroxypropyl cyclodextrin added is 5-10% of the mass of the plant raw material powder, and the amount of glycerophosphate inositol choline salt added is 5-10% of the mass of the plant raw material powder.

[0019] This invention utilizes hydroxypropyl cyclodextrin and glycerophosphoinositol choline salt as auxiliary extraction agents. Glycerophosphoinositol choline salt possesses high hydrophilicity, hydrogen bonding ability, and surface activity, exhibiting excellent chemical penetration effects on frozen and crushed plant materials. Simultaneously, the glycerophosphoinositol anion and choline cation in its structure can better bind with various active ingredients in the plant materials, thereby improving the extraction effect. Furthermore, glycerophosphoinositol choline salt itself, as a skin protectant, possesses anti-inflammatory and soothing effects, synergistically enhancing the soothing, redness-reducing, and repairing effects of the plant extract composition. Hydroxypropyl cyclodextrin, on the one hand, promotes the effective dissolution of active ingredients in the plant materials, improving the extraction effect; on the other hand, it acts as a protectant for the active ingredients in the plant extract and glycerophosphoinositol choline salt, improving extraction and activity stability. Through the synergistic effect of both, the soothing, redness-reducing, and repairing effects of the resulting plant extract composition are enhanced.

[0020] Furthermore, the stirring extraction time in step (2) is 5~12h.

[0021] Furthermore, the concentration in step (2) is preferably carried out under vacuum at a temperature below 60°C to 1 / 5 to 1 / 10 of the original volume, and the filtration and sterilization uses a 0.22μm filter.

[0022] Further, in step (3), the mass ratio of butanediol to hexanediol in the mixed solvent is 100:1~10.

[0023] Furthermore, the amount of the mixed solvent used in step (3) is 0.5 to 1.5 times the mass of the extract.

[0024] Further, the preservative mentioned in step (3) is p-hydroxyacetophenone, and the mass concentration of the preservative added is 0.1~0.5%.

[0025] A redness-reducing and repairing plant extract composition was prepared by the above method.

[0026] Application of the above-mentioned redness-reducing and repairing plant extract composition in cosmetic preparation.

[0027] Compared with the prior art, the beneficial effects of the present invention are:

[0028] (1) The present invention uses hydroxypropyl cyclodextrin and glycerophosphate inositol choline salt to perform low-temperature assisted extraction of plant raw materials, which can significantly improve the soothing, redness-reducing and repairing effects of the obtained plant extract composition.

[0029] (2) The present invention adopts a process of soaking in warm water followed by freezing and grinding, which can ensure the extraction effect of plant active ingredients at low temperature (20~50℃). Attached Figure Description

[0030] Figure 1 The figures show the cell scratch repair effect of the redness-reducing plant extract compositions obtained in Example 1 and Comparative Examples 1-2 on HSF.

[0031] Figure 2 The graph shows the results of the redness-reducing plant extract compositions obtained in Example 1 and Comparative Examples 1-2 promoting the relative expression levels of FLG and LOR genes.

[0032] Figure 3 The graph shows the expression of TRPV1 in the redness-reducing plant extract compositions obtained in Example 1 and Comparative Examples 1-2. Detailed Implementation

[0033] The present invention will be further described in detail below with reference to the embodiments and accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0034] Example 1

[0035] A method for preparing a redness-reducing and repairing plant extract composition includes the following preparation steps:

[0036] (1) Add 50g of dried Centella asiatica leaves, 6g of rosemary, 4g of oat kernels, 4g of peppermint and 1g of Stephania tetrandra to 50-60℃ warm water and soak for 6h. After filtration, add the wet material to a freeze grinder and grind at -70℃ until the powder particle size is 200μm to obtain plant raw material powder.

[0037] (2) Add the obtained plant raw material powder to 500g of deionized water, then add 4g of hydroxypropyl cyclodextrin and 6g of glycerophosphate inositol choline salt and stir to dissolve. Stir and extract at room temperature (20~25℃) for 8h. After filtration, take the filtrate and vacuum concentrate it to about 65ml at 50℃. Filter it with a 0.22μm filter to remove bacteria and obtain the extract.

[0038] (3) Dilute the obtained extract with a mixture of 50 ml butanediol and 2 ml 1,2-hexanediol, and then add 0.25 wt% p-hydroxyacetophenone and mix well to obtain the redness-reducing plant extract composition.

[0039] Example 2

[0040] A method for preparing a redness-reducing and repairing plant extract composition includes the following preparation steps:

[0041] (1) Add 40g of dried Centella asiatica leaves, 8g of rosemary, 2g of oat kernels, 5g of peppermint and 0.5g of Stephania tetrandra to 50-60℃ warm water and soak for 8h. After filtration, add the wet material to a freeze grinder and grind at -80℃ until the powder particle size is 100μm to obtain plant raw material powder.

[0042] (2) Add the obtained plant raw material powder to 400g of deionized water, then add 3g of hydroxypropyl cyclodextrin and 4g of glycerophosphate inositol choline salt and stir to dissolve. Heat to 30~35℃ under water conditions and stir to extract for 10h. After filtration, take the filtrate and vacuum concentrate it to about 55ml at 50℃. Use a 0.22μm filter to filter and sterilize to obtain the extract.

[0043] (3) Dilute the obtained extract with a mixture of 30 ml butanediol and 3 ml 1,2-hexanediol, and then add 0.25 wt% p-hydroxyacetophenone and mix well to obtain the redness-reducing plant extract composition.

[0044] Example 3

[0045] A method for preparing a redness-reducing and repairing plant extract composition includes the following preparation steps:

[0046] (1) Add 30g of dried Centella asiatica leaves, 5g of rosemary, 5g of oat kernels, 2g of peppermint and 2g of Stephania tetrandra to 50-60℃ warm water and soak for 5h. After filtration, add the wet material to a freeze grinder and grind at -60℃ until the powder particle size is 300μm to obtain plant raw material powder.

[0047] (2) Add the obtained plant raw material powder to 300g of deionized water, then add 2.5g of hydroxypropyl cyclodextrin and 3g of glycerophosphate inositol choline salt and stir to dissolve. Heat to 40~45℃ under water conditions and stir to extract for 12h. After filtration, take the filtrate and vacuum concentrate it to about 45ml at 50℃. Use a 0.22μm filter to filter and sterilize to obtain the extract.

[0048] (3) Dilute the obtained extract with a mixture of 60 ml butanediol and 4 ml 1,2-hexanediol, then add 0.25 wt% p-hydroxyacetophenone and mix well to obtain the redness-reducing plant extract composition.

[0049] Example 4

[0050] A method for preparing a redness-reducing and repairing plant extract composition includes the following preparation steps:

[0051] (1) Add 60g of dried Centella asiatica leaves, 10g of rosemary, 2g of oat kernels, 3g of peppermint and 1g of Stephania tetrandra to 50-60℃ warm water and soak for 4h. After filtration, add the wet material to a freeze grinder and grind at -50℃ until the powder particle size is 400μm to obtain plant raw material powder.

[0052] (2) Add the obtained plant raw material powder to 700g of deionized water, then add 5g of hydroxypropyl cyclodextrin and 7.5g of glycerophosphate inositol choline salt and stir to dissolve. Heat to 45~50℃ under water conditions and stir to extract for 5h. After filtration, take the filtrate and vacuum concentrate it to about 75ml at 50℃. Use a 0.22μm filter to filter and sterilize to obtain the extract.

[0053] (3) The obtained extract was diluted with a mixture of 75 ml butanediol and 5 ml 1,2-hexanediol, and then 0.25 wt% p-hydroxyacetophenone was added and mixed well to obtain the redness-reducing plant extract composition.

[0054] Comparative Example 1

[0055] A method for preparing a plant extract composition for reducing redness and repairing redness, compared with Example 1, is that hydroxypropyl cyclodextrin and glycerophosphate inositol choline salt were not added as auxiliary extraction agents in step (2) of the extraction, but the rest is the same.

[0056] Comparative Example 2

[0057] A method for preparing a redness-reducing and repairing plant extract composition includes the following preparation steps:

[0058] Plant raw material powder was prepared using the same method as step (1) in Example 1. The obtained plant raw material powder was added to 500g of deionized water and extracted by stirring at room temperature (20~25℃) for 8h. After filtration, the filtrate was concentrated under vacuum at 50℃ to about 65ml. The filtrate was then filtered through a 0.22μm filter to remove bacteria and obtain the extract. The obtained extract was mixed and dissolved with 4g of hydroxypropyl cyclodextrin and 6g of glycerophosphate inositol choline salt. Then, it was diluted with a mixed solvent of 50ml of butanediol and 2ml of 1,2-hexanediol. 0.25wt% of p-hydroxyacetophenone was added and mixed well to obtain the redness-reducing plant extract composition.

[0059] The performance test results of the redness-reducing and repairing plant extract compositions obtained in Example 1 and Comparative Examples 1-2 are as follows:

[0060] I. Repair Efficacy Test

[0061] The repair efficacy was evaluated using the HSF (human skin fibroblast) scratch repair assay and the skin model FLG (figrin) and LOR (lobeline) promotion assay, respectively.

[0062] (1) Principle of HSF (human skin fibroblast) scratch repair assay: Mechanical damage can lead to a decrease in the migration and repair capacity of fibroblasts, specifically manifested as a decrease in the scratch repair rate. This process is mainly caused by abnormal cell migration and matrix remodeling functions. By systematically detecting the increase in the scratch repair rate after the application of the test substance, it is possible to evaluate whether it has the effect of promoting wound healing and repair, thereby systematically assessing its repair efficacy.

[0063] 1. Reagents and Materials

[0064] 1.1 Culture medium

[0065] DMEM medium containing 10% FBS.

[0066] 1.2 Cultivation Conditions

[0067] Cultured at 37℃, 5% CO2, and saturated humidity.

[0068] 1.3 Solutions and Controls

[0069] Control group: cell culture medium;

[0070] Test samples: diluted to a concentration of 0.5 wt% using culture medium;

[0071] Reagent: CCK-8 cell proliferation assay kit.

[0072] 1.4 Cell lines

[0073] HSF (human skin fibroblasts).

[0074] 2. Test Methods

[0075] 2.1 Sample Preparation

[0076] The test substance was filtered through a 0.22 μm filter membrane and diluted to a concentration of 0.5 wt% with cell culture medium.

[0077] 2.2 Cell Preparation

[0078] HSF cells were cultured in complete culture at 37°C with 5% CO2, and the cell concentration was controlled at 1.0 × 10⁶ cells per mL. 5 ~5.0×10 6 Individual samples were used for biological assays 24–36 hours after passage.

[0079] 2.3 Efficacy Testing

[0080] Cell seeding: HSF cells were resuspended in fresh complete culture medium, diluted with cell culture medium to the seeding density (confluence reached 80% 24 h after seeding), and seeded into culture wells.

[0081] 2.4 Cell scratches

[0082] Drug administration and induction: When cells reached 80% confluence, a sterile 200µl pipette tip was used to scratch the central area of ​​the well plate. The culture medium was discarded, and the cells were washed three times with PBS. The test sample group was given culture medium containing the test substance, while the blank / solvent control group was given only cell culture medium, 2mL per well. At 0h, the scratched cell area was photographed. After drug administration, the 6-well plate was placed in a CO2 incubator for 24h ± 2h. After the cell drug administration was completed, the scratched cell area was photographed. The scratch area was calculated using ImageJ image processing software, and the cell healing rate was calculated according to the following formula:

[0083] .

[0084] 2.5 Statistical Analysis

[0085] Origin was used for plotting, and one-way ANOVA was used for comparisons between groups.

[0086] 2.6 Test Results

[0087] The cell scratch repair effects of the redness-reducing plant extract compositions obtained in Example 1 and Comparative Examples 1-2 on HSF are shown in the figures below. Figure 1 As shown in Table 1 below, the corresponding repair results are as follows.

[0088] Table 1. Results of cell scratch repair of HSF by different groups of redness-reducing plant extract compositions.

[0089]

[0090] Compared with the control group, the sample group showed *p<0.05 and **p<0.01.

[0091] From Table 1, Figure 1 It can be seen that the repair rates of the test substances in Comparative Example 1, Comparative Example 2, and Example 1 on HSF cell scratches were 3.23%, 7.35%, and 18.66%, respectively. This indicates that the low-temperature assisted extraction of plant raw materials using hydroxypropyl cyclodextrin and glycerophosphate inositol choline salt can significantly improve the repair efficacy of the obtained plant extract composition.

[0092] (2) Principle of skin model FLG (figrin) and LOR (lobeline) promotion test: Blue light (UVB) + Irradiation can induce a significant downregulation of the expression levels of genes encoding terminal differentiation markers FLG (figrin) and LOR (lobeline) in keratinocytes. This process is mainly mediated by blue light / UVB radiation. +Activated AP-1 transcription factor and p38MAPK signaling pathway inhibit FLG and LOR promoter activity and interfere with retinoic acid receptor (RAR) signaling, among other mechanisms. By detecting the mRNA or protein expression levels of FLG and LOR after treatment with the test substance, its ability to antagonize the blue light UVB-induced keratinization capsule assembly disorder can be evaluated, thereby assessing its potential to repair skin barrier function and resist photoaging.

[0093] 1. Reagents and Materials

[0094] 1.1 Model: EpiDermFT full-skin model.

[0095] 1.2 Culture medium: DMEM containing 2 mL of glutamine.

[0096] 1.3 Culture conditions: Cultured at 37℃, 5% CO2, and saturated humidity.

[0097] 1.4 Solutions and Controls

[0098] Blank group: culture medium.

[0099] Model group: Blue light (UVB) + Irradiation.

[0100] Test sample: diluted to a concentration of 2.5 wt% using culture medium.

[0101] 1.5 Reagents: RNA extraction kit, second-generation reverse transcription kit, ChamQ Universal SYBR gPCRMaster Mix (PCR amplification detection kit).

[0102] 2. Test Methods

[0103] 2.1 Sample Preparation

[0104] The test substance was filtered through a 0.22 μm filter membrane and diluted to a concentration of 2.5 wt% with cell culture medium.

[0105] 2.2 Model Preparation

[0106] The EpiDermFT full-skin model was placed in a 6-well plate, 1 ml of Assay Medium was added, and the plate was incubated at 37°C and 5% CO2 for 24 hours before recovery and use.

[0107] 2.3 Efficacy Testing

[0108] Drug administration and induction: The EpiDemFT full-skin model was placed in a 12-well plate, and 1 mL of Assay Medium was added. The test sample group was given culture medium containing the test substance, while the blank control group was given only sterile deionized water. Each group was divided into two replicates, with 1 mL per well. After drug administration, the plates were incubated in a CO2 incubator for 24 h.

[0109] LOR test: After drug administration, EpiDermFT whole skin model RNA was extracted according to the instructions of the RNA extraction kit, and the extracted RNA was converted into cDNA according to the instructions of the second-generation reverse transcription kit. Finally, appropriate primers were selected according to the instructions of the ChamQUniversal SYBR gPCR Master Mix (PCR amplification detection kit) to perform PCR amplification and detect gene expression level.

[0110] FLG test: After drug administration, EpiDermFT whole skin model RNA was extracted according to the instructions of the RNA extraction kit, and the extracted RNA was converted into cDNA according to the instructions of the second-generation reverse transcription kit. Finally, appropriate primers were selected according to the instructions of the ChamQUniversal SYBR gPCR Master Mix (PCR amplification detection kit) to perform PCR amplification and detect gene expression levels.

[0111] 2.4 Statistical Analysis

[0112] Origin was used for plotting, and one-way ANOVA was used for comparisons between groups.

[0113] 2.5 Test Results

[0114] The results of the redness-reducing plant extract compositions obtained in Example 1 and Comparative Examples 1-2 promoting the relative expression levels of FLG and LOR genes are shown in the figure. Figure 2 As shown in the figure, the corresponding results are shown in Table 2 below.

[0115] Table 2. Results of the effects of different groups of redness-repairing plant extract compositions on the relative expression levels of FLG and LOR genes.

[0116]

[0117] Compared with the model group: *p<0.05, **p<0.01.

[0118] From Table 2, Figure 2It can be seen that the promotion rates of LOR expression in the EpiDermFT whole-skin model by the test substances of Comparative Examples 1, 2, and 1 were 18.18%, 35.23%, and 60.71%, respectively, and the promotion rates of FLG expression were 15.35%, 22.28%, and 48.84%, respectively. This indicates that the low-temperature assisted extraction of plant raw materials using hydroxypropyl cyclodextrin and glycerophosphate inositol choline salt in this invention can significantly improve the repair efficacy of the obtained plant extract composition.

[0119] II. Soothing Efficacy Test

[0120] Inhibition assay using TRPV1 (transient receptor potential vanillic acid isoform 1): UVB + The stimulation model leads to overactivation of sensory nerve receptors in fibroblasts, specifically manifested as upregulation of transient receptor potential vanillic acid isoform 1 (TRPV1) expression and increased release of downstream inflammatory mediators, accompanied by inhibition of extracellular matrix remodeling-related protein synthesis. This process is mainly mediated by neurogenic inflammatory pathways and pain-sensitivity signaling networks. By systematically detecting changes in TRPV1 protein expression in cells after treatment with the test substance, its ability to inhibit neurogenic inflammation and reduce skin sensitivity can be evaluated, thereby systematically assessing its soothing efficacy.

[0121] 1. Reagents and Materials

[0122] 1.1 Culture medium

[0123] DMEM medium containing 10% FBS.

[0124] 1.2 Cultivation Conditions

[0125] Cultured at 37℃, 5% CO2, and saturated humidity.

[0126] 1.3 Solutions and Controls

[0127] Control group: cell culture medium;

[0128] Model group: UVB + blue light irradiation;

[0129] Test samples: Dilute to the required test concentration using culture medium;

[0130] Reagents: CCK-8 cell proliferation assay kit, TRPV1 assay kit.

[0131] 1.4 Cell lines

[0132] HSF (human skin fibroblasts).

[0133] 2. Operation process

[0134] 2.1 Sample Preparation

[0135] The test substance was filtered through a 0.22 μm filter membrane and diluted to a concentration of 0.5 wt% with cell culture medium.

[0136] 2.2 Cell Preparation

[0137] HSF cells were cultured in complete culture at 37°C with 5% CO2, and the cell concentration was controlled at 1.0 × 10⁶ cells per mL. 5 ~5.0×10 6 Individual samples were used for biological assays 24–36 hours after passage.

[0138] 2.3 Efficacy Testing

[0139] Cell seeding: HSF cells were resuspended in fresh complete culture medium and diluted with cell culture medium to the seeding density (80% confluence 24 hours after seeding) and seeded into 96-well plates. Once cells reached 80% confluence, the culture medium in the 96-well plates was replaced with PBS. The cell culture plates were then exposed to blue light (except for the blank / solvent control group, which was shielded with aluminum foil; all other groups were exposed to UVB). + Under blue light, at a distance of 10cm from the light source, the irradiation intensity is 60mJ / cm². 2 PBS was then discarded. Test samples were added to culture medium containing a certain concentration of the test substance, while the blank / solvent control group and negative control group were added only to cell culture medium, 100 μL per well. The 96-well plates were then incubated in a CO2 incubator for 24 h ± 2 h. After drug administration, TRPV1 expression was tested according to the TRPV1 detection kit instructions.

[0140] 2.4 Statistical Analysis

[0141] Origin was used for plotting, and one-way ANOVA was used for comparisons between groups.

[0142] 2.5 Test Results

[0143] The expression of TRPV1 by the redness-reducing plant extract compositions obtained in Example 1 and Comparative Examples 1-2 is shown in the figure below. Figure 3 As shown in the figure, the corresponding results are shown in Table 3 below.

[0144] Table 3. Results of TRPV1 expression in each group of redness-repairing plant extract compositions.

[0145]

[0146] Compared with the model group: *p<0.05, **p<0.01.

[0147] From Table 3, Figure 3It can be seen that the inhibition rates of the test substances in Comparative Example 1, Comparative Example 2, and Example 1 on TRPV1 expression in HSF cells were 40.52%, 58.93%, and 74.51%, respectively. This indicates that the low-temperature assisted extraction of plant raw materials using hydroxypropyl cyclodextrin and glycerophosphate inositol choline salt can significantly improve the soothing efficacy of the obtained plant extract composition.

[0148] Example 5

[0149] A redness-reducing and repairing serum has the following formula composition as shown in Table 4.

[0150] Table 4. Formula of Redness Reducing and Repairing Serum

[0151]

[0152] The preparation method of this redness-reducing and repairing serum is as follows:

[0153] Add ingredients 1-7, heat to 80-85℃, homogenize for 3-5 minutes until the product is free of particles, obtaining phase A; cool to about 50℃, add ingredients 8-12 of phase B and stir evenly, then cool to 45℃, add ingredients C and stir evenly to obtain the redness-reducing and repairing essence. The redness-reducing and repairing plant extract compositions of Comparative Examples 1 and 2 were prepared into redness-reducing and repairing essences using the same method described above.

[0154] The redness-reducing and repairing essence was tested for its efficacy in soothing redness on the human body. The testing method is as follows:

[0155] (1) On the first visit, explain the trial to the subjects and obtain their informed consent.

[0156] (2) The inner forearm should not come into contact with water before the visit. Before the experiment, the subjects need to clean the inner forearms of both hands uniformly. The method of cleaning the arms is to wipe them clean with a dry tissue. Three measurement areas are marked on the inner forearm of the subject's left or right hand. Each area is 3cm*3cm. The test areas are spaced at least 1cm apart. The sample area and the negative area should be randomly distributed in the marked area to ensure that the position of each area is statistically balanced.

[0157] (3) The subject’s exposed arm rested for 30 minutes in a constant temperature and humidity room.

[0158] (4) Use Antera3D to test the average reddish hue of the red area in the calibration region.

[0159] (5) Apply the inducer (0.1% histamine solution) to the sample area and negative area for 10 min.

[0160] (6) Before using the sample: use Antera3D to test the average reddish hue of the red area in each region; the subject fills out a self-assessment questionnaire.

[0161] (7) Use the sample: Use the sample in the sample area according to the method of use according to the random table.

[0162] (8) After using the product for 5 minutes: use Antera3D to test the average reddish hue of the red area in each region.

[0163] (9) Subjects fill out a self-assessment questionnaire within 5 minutes of receiving the sample.

[0164] Table 5. Test results of the redness-reducing and repairing serums in each group on the human body's soothing redness effect.

[0165]

[0166] As shown in Table 5, the differences in the soothing redness efficacy of the essences prepared from the plant extract compositions obtained in Comparative Examples 1, 2, and 1 of Example 1 compared to the negative control area were 5.88%, 7.64%, and 11.52%, respectively. This indicates that the low-temperature assisted extraction of plant raw materials using hydroxypropyl cyclodextrin and glycerophosphate inositol choline salt can significantly improve the soothing redness efficacy of the obtained plant extract compositions in formulation applications.

[0167] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A method for preparing a redness-reducing and repairing plant extract composition, characterized in that... The preparation steps include the following: (1) The dried Centella asiatica leaves, rosemary, oat kernels, peppermint and Stephania tetrandra raw materials were soaked in warm water and then frozen and ground to obtain plant raw material powder; (2) Add the obtained plant raw material powder to deionized water, then add hydroxypropyl cyclodextrin and glycerophosphate inositol choline salt as auxiliary extraction agents, stir and extract at 20~50℃, concentrate and filter to remove bacteria to obtain the extract; (3) The obtained extract was diluted with a mixed solvent of butanediol and hexanediol, and then a preservative was added and mixed well to obtain a redness-reducing plant extract composition.

2. The method for preparing a redness-reducing and repairing plant extract composition according to claim 1, characterized in that: The mass ratio of Centella asiatica leaves, rosemary, oat kernels, peppermint and Stephania tetrandra in step (1) is as follows: Centella asiatica leaves 30-60 parts, rosemary 5-10 parts, oat kernels 2-5 parts, peppermint 2-5 parts, Stephania tetrandra 0.5-2 parts.

3. The method for preparing a redness-reducing and repairing plant extract composition according to claim 1, characterized in that: The warm water soaking mentioned in step (1) is to soak in warm water at 50-60℃ for 4-8 hours, and the freeze grinding refers to grinding at -50~-80℃ until the powder particle size is 50~500μm.

4. The method for preparing a redness-reducing and repairing plant extract composition according to claim 1, characterized in that: The mass ratio of plant raw material powder to deionized water in step (2) is 1:6~12.

5. The method for preparing a redness-reducing and repairing plant extract composition according to claim 1, characterized in that: The amount of hydroxypropyl cyclodextrin added in step (2) is 5-10% of the mass of the plant raw material powder, and the amount of glycerophosphoinositol choline salt added is 5-10% of the mass of the plant raw material powder.

6. The method for preparing a redness-reducing and repairing plant extract composition according to claim 1, characterized in that: The stirring and extraction time in step (2) is 5~12h.

7. The method for preparing a redness-reducing and repairing plant extract composition according to claim 1, characterized in that: The concentration in step (2) is carried out under vacuum at a temperature below 60°C to 1 / 5 to 1 / 10 of the original volume, and the filtration and sterilization are carried out using a 0.22μm filter.

8. The method for preparing a redness-reducing and repairing plant extract composition according to claim 1, characterized in that: In step (3), the mass ratio of butanediol to hexanediol in the mixed solvent is 100:1~10, the amount of the mixed solvent is 0.5~1.5 times the mass of the extract, the preservative is p-hydroxyacetophenone, and the mass concentration of the preservative added is 0.1~0.5%.

9. A plant extract composition for reducing redness and repairing skin redness, characterized in that: It is prepared by the method described in any one of claims 1 to 8.

10. The application of the redness-reducing and repairing plant extract composition according to claim 9 in the preparation of cosmetics.

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