Glycosylated carbon dots based on genipin and their preparation method and application

Abstract

This invention discloses a genipin-based traditional Chinese medicine-derived carbon dot, its preparation method, and its application, belonging to the field of traditional Chinese medicine nanomaterials technology. It aims to solve the problems of existing anti-Parkinson's disease drugs failing to halt disease progression and the difficulty of traditional Chinese medicine active ingredients crossing the blood-brain barrier. The carbon dot is prepared by dissolving genipin and tryptophan in a mass ratio of 1:0.5-1:2 and then reacting them in a microwave hydrothermal environment at 170℃-190℃ for 1-3 hours. This carbon dot has a small particle size, good water solubility, and can efficiently cross the blood-brain barrier, exhibiting a dual-effect synergistic neuroprotective effect. It can be used to prepare drugs for the prevention and / or treatment of Parkinson's disease.

Description

Technical Field

[0001] This invention relates to the field of nanomaterials technology for traditional Chinese medicine. More specifically, this invention relates to a carbon dot derived from traditional Chinese medicine based on genipin, its preparation method, and its application. Background Technology

[0002] Parkinson's disease (PD) is a common neurodegenerative disease of the central nervous system, characterized by the progressive loss of dopaminergic neurons in the substantia nigra and striatum, and the abnormal deposition of Lewy bodies (mainly composed of aggregated α-synuclein) within cells. The pathogenesis involves multiple aspects, including oxidative stress, mitochondrial dysfunction, neuroinflammation, and impaired protein degradation pathways. Currently, widely used drugs (such as levodopa and its derivatives) are mainly used to supplement dopamine to alleviate motor symptoms, but they cannot reverse or stop the progressive degeneration of dopaminergic neurons, and long-term use can easily lead to serious side effects such as dyskinesia.

[0003] Traditional Chinese medicine (TCM) possesses unique advantages in multi-target neuroprotection, anti-oxidation, and anti-neuroinflammatory effects. However, most active ingredients in TCM suffer from drawbacks such as poor water solubility, rapid in vivo metabolism, and low bioavailability. Particularly due to the blood-brain barrier (BBB), it is extremely difficult for TCM monomers to achieve effective therapeutic concentrations within the central nervous system. Carbon dots (CDs), as a novel type of zero-dimensional carbon nanomaterial, possess characteristics such as small particle size (typically less than 10 nm), easy surface functionalization, good water solubility, and excellent biocompatibility. Recent studies have shown that certain small-particle-size nanomaterials have the potential to cross the blood-brain barrier. Therefore, combining the active ingredients of traditional Chinese medicine with carbon dot nanotechnology, and using the Chinese medicine itself as a carbon source to prepare Chinese medicine-derived carbon dots through a green synthesis method, can combine the deep tissue penetration advantage of nanomaterials with the multi-target pharmacological activity of Chinese medicine. This is expected to be an effective strategy for developing new anti-Parkinson's disease drugs. However, there are currently no literature reports on using genipin and tryptophan as precursors to prepare Chinese medicine-derived carbon dots and using them for the prevention and / or treatment of Parkinson's disease. Summary of the Invention

[0004] The purpose of this invention is to provide a traditional Chinese medicine-derived carbon dot based on genipin, its preparation method and application, in order to solve the technical problems that existing anti-Parkinson's disease drugs cannot stop the disease progression and that the active ingredients of traditional Chinese medicine are difficult to cross the blood-brain barrier.

[0005] To achieve the objectives and other advantages of this invention, a traditional Chinese medicine-derived carbon dot based on genipin is provided. This carbon dot is obtained by mixing and dissolving genipin and tryptophan and then subjecting the mixture to a microwave hydrothermal reaction. The mass ratio of genipin to tryptophan is 1:0.5-1:2, and the reaction temperature of the microwave hydrothermal reaction is 170℃-190℃, with a reaction time of 1-3 hours.

[0006] This invention also provides a method for preparing traditional Chinese medicine-derived carbon dots based on genipin, comprising the following steps: S1. Mix and dissolve genipin and tryptophan in a mass ratio of 1:0.5-1:2, and stir until homogeneous to obtain mixed solution A; S2. Mixed solution A is subjected to a microwave hydrothermal reaction at a temperature of 170℃-190℃ for 1-3 hours to obtain mixed solution B. S3. The mixed solution B is purified by separation, dialysis, and freeze-dried to obtain carbon dots derived from traditional Chinese medicine.

[0007] Preferably, in S2, the reaction temperature is 180°C and the reaction time is 2 hours.

[0008] Preferably, in S3, the separation and purification are performed by filtration through a 0.22 μm aqueous filter membrane, and the filtrate is centrifuged at 10000-12000 rpm for 10 min, and the supernatant is collected.

[0009] Preferably, in step S3, the purified solution is placed in a dialysis bag and dialyzed for 24 hours, with the dialysate being replaced every 12 hours. The dialysate is deionized water, and the molecular weight cutoff of the dialysis bag is 1000 Da.

[0010] The present invention also provides the application of genipin-based traditional Chinese medicine-derived carbon dots in the preparation of drugs for the prevention and / or treatment of Parkinson's disease.

[0011] The present invention has at least the following beneficial effects: 1. Green synthesis and high safety: This invention uses genipin and tryptophan, extracts of traditional Chinese medicine, as precursors. There is no need to add strong acids, strong bases or toxic chemical passivation reagents, which ensures the biosafety of nanomaterials from the source and has extremely low cytotoxicity.

[0012] 2. High efficiency in penetrating the blood-brain barrier: The carbon dots prepared by this invention have a uniform particle size distribution, with a size of <5 nm, and retain the structural characteristics of traditional Chinese medicine extracts on their surface, effectively solving the problem of low brain penetration rate of traditional neurodegenerative disease drugs.

[0013] 3. Dual-effect synergistic neuroprotection: This carbon dot can act as a free radical scavenger to significantly reduce ROS levels in dopaminergic neurons.

[0014] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Attached Figure Description

[0015] Figure 1 This is a diagram showing the optimized results of the carbon dot preparation method in Example 1 of the present invention; Figure 2 These are characterization diagrams of the GT-CDs prepared in this invention; Figure 3 This is a graph showing the experimental results of the reactive oxygen species scavenging ability of the GT-CDs prepared in this invention; Figure 4 This is a graph showing the results of the GT-CDs prepared in this invention in terms of their stability in scavenging reactive oxygen species and their biosafety. Figure 5 This is a figure showing the experimental results of verifying the function of GT-CDs prepared in this invention on PC12 cells in mice; Figure 6 This is a graph showing the behavioral experimental results of the therapeutic effect of the GT-CDs prepared in this invention on a Parkinson's mouse model; Figure 7 The figure shows the effect of the GT-CDs and Genipin+Trp prepared in this invention on the ABTS free radical scavenging rate. Detailed Implementation

[0016] The present invention will now be described in further detail with reference to the embodiments and accompanying drawings, so that those skilled in the art can implement it based on the description.

[0017] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0018] It should be noted that, unless otherwise specified, the experimental methods described in the following implementation plan are all conventional methods, and the reagents and materials described are all commercially available unless otherwise specified.

[0019] Example 1: Optimization of carbon dot preparation method.

[0020] The preparation steps of traditional Chinese medicine-derived carbon dots based on genipin include: S1. Weigh out genipin and tryptophan; S2. Using deionized water as a solvent, genipin and tryptophan are fully dissolved under heating conditions to obtain mixed solution A; S3. Transfer the mixed solution A obtained in S2 to a microwave hydrothermal reactor to carry out a hydrothermal reaction to obtain mixed solution B; S4. Filter the mixed solution B obtained in S3 through a 0.22 μm aqueous filter membrane and centrifuge (10000 rpm, 10 min) to obtain solution C; S5. Place the solution C obtained in S4 into a dialysis bag with a molecular weight cutoff of 1000 Da and dialyze for 24 hours, changing the dialysate every 12 hours. The dialysate is deionized water. After the process is completed, put the sample into a centrifuge tube and freeze it in an ultra-low temperature freezer at -80℃ for more than 4 hours. Then transfer it to a freeze dryer pre-cooled to -40℃ and dry it overnight to obtain the carbon dots of traditional Chinese medicine.

[0021] The optimization was carried out using single-factor experiments. The hydrothermal reaction time was 1 hour, 2 hours, and 3 hours (with a fixed reaction temperature of 170℃ and a mass ratio of 1:1). The hydrothermal reaction temperature was 170℃, 180℃, and 190℃ (with a fixed reaction time of 2 hours and a mass ratio of 1:1). The mass ratio of genipin to tryptophan was 1:2, 1:1, and 2:1. The total mass of genipin and tryptophan was 200 mg. The volume of solvent was 30 mL (with a fixed reaction time of 2 hours and a reaction temperature of 180℃). The test index was ABTS free radical scavenging rate. The specific test method was as follows: First, ABTS free radical solution was prepared by mixing 7 mM ABTS aqueous solution with 2.45 mM potassium persulfate and incubating for 12 hours. The solution was then diluted with phosphate-buffered saline (PBS) to obtain ABTS working solution. Carbon dot solutions generated under different synthesis conditions were added and mixed 1:1 with the ABTS working solution. After 20 minutes, the absorbance at 734 nm was measured using a UV-Vis spectrophotometer. The antioxidant capacity was evaluated by calculating the ABTS free radical scavenging rate. ABTS free radical cationic scavenging activity = (A0 - A) / A0 × 100%. (A0 is the absorbance of the ABTS solution without the sample; A is the absorbance after the sample reacts with ABTS). The test results are as follows: Figure 1 As shown.

[0022] Depend on Figure 1 It can be seen that, within a reaction time of 2 hours ( Figure 1 (A)), reaction temperature 180℃ ( Figure 1 (B) The mass ratio of genipin to tryptophan is 1:1. Figure 1 Under the conditions specified in (C), the reactive oxygen species scavenging ability of carbon dots was the strongest. The final synthesis conditions for genipin-based traditional Chinese medicine-derived carbon dots were determined to be: m(genipin):m(tryptophan) = 1:1, reaction time = 2 h, and reaction temperature = 180℃. Subsequent experiments were conducted using genipin-based traditional Chinese medicine-derived carbon dots GT-CDs synthesized under these reaction conditions.

[0023] Experimental Example 1: Characterization of GT-CDs.

[0024] GT-CDs were tested using transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD), respectively. The results are as follows: Figure 2 As shown. From Figure 2 (A) The TEM results show that GT-CDs are spherical, well-dispersed, and have an average particle size of less than 5 nm; from Figure 2 The AFM results in (B) show that GT-CDs are well dispersed in water, with no obvious aggregation, and the thickness is concentrated in the range of 2-4 nm; Figure 2 The XPS results in (C) show that GT-CDs contain peaks of C1S, O1S, and N1S, indicating that GT-CDs are mainly composed of carbon, nitrogen, and oxygen elements, suggesting that their surface is enriched with hydrophilic groups, including amino and carboxyl groups; from Figure 2 The XRD results of (D) show that GT-CDs are mainly composed of amorphous carbon, which is consistent with the characteristics of the (002) crystal plane of graphite.

[0025] Experimental Example 2: Reactive oxygen species scavenging ability of GT-CDs.

[0026] The reactive oxygen species (ROS) scavenging capacity of GT-CDs was characterized using ABTS scavenging assays, hydroxyl radical scavenging assays, and superoxide anion assays, respectively. The test method for ABTS free radical scavenging rate is the same as in Example 1.

[0027] Test method for hydroxyl radical scavenging rate: The scavenging ability of hydroxyl radicals (·OH) was detected by the TMB colorimetric method. 0.0769 g of 3,3',5,5'-tetramethylbenzidine (TMB) was dissolved in 2 mL of dimethyl sulfoxide to prepare the TMB working solution. Then, the following were added sequentially: 1.97 mL of GT-CDs solutions of different concentrations, 10 μL of FeSO4, 10 μL of hydrogen peroxide solution, and 10 μL of TMB working solution. After mixing, the mixture was reacted at 25°C in the dark for 10 minutes. Finally, the absorbance at 652 nm was measured using a UV-Vis spectrophotometer. The antioxidant capacity was evaluated by calculating the hydroxyl radical scavenging rate. Hydroxyl radical scavenging capacity = (A0-A) / A0 × 100%, where A and A0 are the absorbances with and without GT-CDs, respectively.

[0028] Superoxide anion scavenging rate was tested using an SOD assay kit. NBT working solution preparation: Met buffer and NBT solution were mixed at a ratio of 23:3. Different concentrations of GT-CDs solutions were mixed with the NBT working solution and FD solution and transferred to 2 mL tubes. The centrifuge tubes were then exposed to natural light for 10 minutes. Finally, the absorbance at 560 nm was measured using a UV-Vis spectrophotometer. Dark treatment group: The same system was used for the reaction in the dark. Superoxide anion free radicals (O... 2− Clearance (%) = (A0-A) / A0 × 100%, where A and A0 are the absorbance with and without GT-CDs, respectively.

[0029] Experimental results are as follows Figure 3 As shown. By Figure 3 (A) Figure 3 (B) indicates that GT-CDs possess excellent ABTS radical scavenging ability, exhibiting both time- and concentration-dependent characteristics. Figure 3 (C) indicates that GT-CDs possess excellent hydroxyl radical scavenging ability, and this ability is concentration-dependent. Figure 3 (D) indicates that GT-CDs have good superoxide anion scavenging ability, and this ability is concentration-dependent.

[0030] Experimental Example 3: Stability and biosafety of GT-CDs.

[0031] Stability was tested by measuring the ABTS free radical scavenging rate of GT-CDs for seven consecutive days; biosafety of GT-CDs was tested by hemolysis experiments and blood biochemical indicators.

[0032] 1. ABTS Stability: The ABTS radical scavenging rate of GT-CDs was measured for seven consecutive days, and the results are as follows: Figure 4 As shown in (A). Figure 4 (A) It can be seen that GT-CDs have good free radical scavenging ability and stability for seven consecutive days, which is beneficial for biological applications; 2. Hemolysis Assay: Blood was collected from the mouse orbital cavity, and the red blood cells were washed three times with PBS to prepare a red blood cell suspension. GT-CDs were dissolved in the red blood cell suspension to prepare GT-CDs red blood cell suspensions of different concentrations, and incubated at 37°C for 2 hours. Deionized water and PBS were used as positive and negative controls, respectively. After incubation, the cells were centrifuged, and the absorbance of the supernatant was measured at 570 nm. Hemolysis rate (%) = (sample OD - negative control OD) / (positive control OD - negative control OD) × 100%. The results are shown below. Figure 4 As shown in (B). Figure 4 (B) It can be seen that GT-CDs do not cause hemolytic reactions.

[0033] 3. Blood Biochemical Detection: MPTP-induced PD mouse model construction: All mice were purchased from Beijing Huafukang Biotechnology Co., Ltd., and were male C57BL / 6 mice, approximately 6 weeks old. After being housed under constant temperature conditions for one week, they were randomly divided into 4 groups: control group, PD group, PD + genipin treatment group, and PD + GT-CDs treatment group. MPTP (25 mg / kg) was injected intraperitoneally for 7 consecutive days, while the control group was injected with an equal volume of physiological saline. The treatment group was administered MPTP via nasal administration (1 mg / mL, single administration volume 10 μL, total administration dose 0.5 mg / kg) for 3 days before the start of MPTP injection, 3 days and 6 days after the start of MPTP injection, and 2 days after the end of MPTP injection. Blood was collected from the orbital sinus after behavioral testing, and then centrifuged at 4℃, 3000rpm for 5 minutes. The upper light yellow serum was collected, and blood biochemical indicators were detected using ALT, AST, and blood urea nitrogen (BUN) kits.

[0034] ALT assay: 20 μL of matrix solution and 5 μL of the sample to be tested were added to a 96-well plate, the plate was gently shaken to mix, and the mixture was incubated at 37°C for 30 minutes. Then, 20 μL of 2,4-dinitrophenylhydrazine solution was added, the plate was gently shaken to mix, and the mixture was incubated at 37°C for 20 minutes. Finally, 200 μL of 0.4 mol / L sodium hydroxide solution was added, the mixture was incubated at room temperature for 15 minutes, and the OD value of each well was measured using a microplate reader at a wavelength of 505 nm. The values ​​were then substituted into the standard curve to obtain the corresponding ALT activity values. AST assay: Add 20 μL of aspartate aminotransferase substrate solution and 5 μL of the sample to be tested to 96 wells, gently shake the plate to mix, and react at 37°C for 30 minutes; then add 20 μL of 2,4-dinitrophenylhydrazine solution and mix, and continue to react at 37°C for 20 minutes; finally add 200 μL of 0.4 mol / L sodium hydroxide solution and mix; incubate at room temperature for 15 minutes, and measure the OD value of each well at a wavelength of 510 nm using a microplate reader. Substitute the values ​​into the standard curve to calculate the corresponding AST activity value. BUN detection: Mix the buffer enzyme solution (0.25 mL) with the sample to be tested (0.02 mL) and incubate at 37°C for 10 minutes. Then add phenol chromogenic reagent (1 mL) and alkaline sodium hypochlorite (1 mL) and mix thoroughly. Incubate at 37°C for 10 minutes. The wavelength is 640 nm and the optical path is 1 cm. Zero the instrument with double distilled water and measure the absorbance (OD) value of each tube. Substitute the values ​​into the formula to obtain the BUN concentration.

[0035] The results are as follows Figure 4 (C) Figure 4 (D) and Figure 4 As shown in (E), blood biochemical indicators obtained from mice after intranasal administration of the drug demonstrated that, compared with the control group, the genipin and GT-CDs groups had no effect on liver / kidney function.

[0036] Experiment Example 4: Validation experiment of GT-CDs on PC12 function in mouse cells.

[0037] 1. MTT assay: PC12 cells in logarithmic growth phase were seeded in 96-well plates. After adhesion, different concentrations of GT-CDs solution were added for treatment. After 24 hours, the drug-containing medium was discarded, and 100 μL of MTT solution was added. The cells were incubated at 37°C for 4-6 hours. Subsequently, the waste liquid was discarded, and 150 μL of DMSO solution was added. After incubation at 37°C for 10 minutes, the absorbance was measured using a microplate reader, and the cell viability was calculated. The results are shown below. Figure 5 As shown in (A). Figure 5 (A) indicates that GT-CDs exhibit good biocompatibility in PC12 cells. A Parkinson's cell model was then constructed using rotenone ROT, and cell viability was measured using the same method described above. The results are as follows: Figure 5 (B) Figure 5 As shown in (C). Figure 5 (B) Figure 5 (C) indicates that PC12 cells were damaged after ROT-induced PD model, but the addition of GT-CDs (60 μg / mL) could save the cells from death, and the effect was better than that of the raw material Genipin (60 μg / mL).

[0038] 2. Calcein AM-PI Assay: PC12 cells in logarithmic growth phase were seeded in 48-well plates. After adhesion, different drugs (genipin, 60 μg / mL and GT-CDs, 60 μg / mL) were added for pretreatment for 4 hours. Then, 1 μM MROT was added, and the cells were incubated for another 24 hours. After discarding the drug-containing medium, Calcein AM-PI was added for staining in the dark for half an hour. The staining solution was washed off with PBS, and the results were photographed. Figure 5 As shown in (D). Figure 5 (D) indicates that GT-CDs can improve cell viability significantly more than the raw material genipin. Figure 5 (D) The control group received an equal volume of PBS, while the "-" group received only 1 μM ROT. 3. Reactive Oxygen Species (ROS) Detection: PC12 cells in logarithmic growth phase were seeded in 48 wells. After adhesion, different drugs (genipin (60 μg / mL) and GT-CDs (60 μg / mL)) were added for pretreatment for 4 hours. Then, 1 μM ROT was added, and the cells were incubated for another 24 hours. After discarding the drug-containing medium, DCFH-DA was added for staining in the dark for half an hour. The staining solution was washed off with PBS, and the results were photographed. Figure 5 As shown in (E). Figure 5 (E) indicates that GT-CDs have good reactive oxygen species scavenging ability and are significantly higher than that of the raw material genipin. Figure 5 In (E), the control group received an equal volume of PBS, while the "-" group received only 1 μM ROT. Experiment Example 5: Mouse Behavioral Experiment.

[0039] MPTP-induced PD mouse model construction: All mice were purchased from Beijing Huafukang Biotechnology Co., Ltd., and were male C57BL / 6 mice, approximately 6 weeks old. After being housed under constant temperature conditions for one week, they were randomly divided into 4 groups: control group, PD group, PD + genipin treatment group, and PD + GT-CDs treatment group. MPTP (25 mg / kg) was injected intraperitoneally for 7 consecutive days, while the control group was injected with an equal volume of physiological saline. The treatment group was administered the drug (nasal administration, 1 mg / mL, single administration volume 10 μL, total administration dose 0.5 mg / kg) three days before the start of MPTP injection, on the third and sixth days after the start of MPTP injection, and two days after the end of MPTP injection. Behavioral tests were conducted one week after the model was established.

[0040] 1. Open field test: Prepare a 40×40×40 cm box in advance. Record the movement trajectory of the mouse using video. Analyze the data within 10 minutes, including the total distance the mouse moves, the total distance it moves in the central area, the average speed, the total dwell time, and the number of times it enters the central area. The results are as follows: Figure 6 (A) to Figure 6 As shown in (F). The results show that the PD model group mice were slow to move. After treatment, the slowness of movement was improved, the total distance increased and the speed improved, indicating that GT-CDs have a good therapeutic effect and are superior to genipin; 2. Pole Climbing Experiment: A solid ball with a diameter of approximately 2.5 cm was fixed to a 50 cm long wooden pole. A layer of gauze was wrapped around the ball to prevent slipping. A mouse was placed head-up on the top ball. The total time taken for the mouse to climb from the top to the bottom of the pole and the time taken to complete a 180° turn at the top were recorded. The results are as follows: Figure 6 (H) and Figure 6 As shown in (I). The results show that the mice's sluggishness improved after treatment, proving that GT-CDs have a good therapeutic effect and are superior to the genipin group; 3. Rotating bar test: Place the mouse on a rotating wooden bar with gradually increasing speed. The rotation speed is increased to 30 rpm within 3 minutes. Record the time it takes for the mouse to fall. The results are as follows: Figure 6 As shown in (G). The results show that the PD model group mice fell off the ground the fastest, and the time was significantly improved after treatment, proving that GT-CDs have a good therapeutic effect and are superior to the genipin group.

[0041] To further highlight the effectiveness of the GT-CDs prepared in this invention, during the experiment, the GT-CDs (concentration of 100 ug / ml) were compared with a mixture of Genipin+Trp (genipin and tryptophan were prepared at the same mass ratio and concentration as when GT-CDs were prepared) to test the ABTS radical scavenging rate of both. The results are as follows: Figure 7 As shown. By Figure 7 It can be seen that the ABTS radical scavenging rate of GT-CDs prepared by this invention is significantly higher than that of Genipin+Trp.

[0042] The number of devices and processing scale described herein are for simplification of the invention. Applications, modifications, and variations of this invention based on genipin-derived carbon dots from traditional Chinese medicine and their applications will be readily apparent to those skilled in the art.

[0043] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and illustrations shown and described herein.

Claims

1. A traditional Chinese medicine-derived carbon dot based on genipin, characterized in that, It is obtained by mixing and dissolving genipin and tryptophan and then carrying out a microwave hydrothermal reaction. The mass ratio of genipin to tryptophan is 1:0.5-1:2, the reaction temperature of the microwave hydrothermal reaction is 170℃-190℃, and the reaction time is 1-3 hours.

2. A method for preparing carbon dots derived from traditional Chinese medicine based on genipin, characterized in that, Includes the following steps: S1. Mix and dissolve genipin and tryptophan in a mass ratio of 1:0.5-1:2, and stir until homogeneous to obtain mixed solution A; S2. Mixed solution A is subjected to a microwave hydrothermal reaction at a temperature of 170℃-190℃ for 1-3 hours to obtain mixed solution B. S3. The mixed solution B is purified by separation, dialysis, and freeze-dried to obtain carbon dots derived from traditional Chinese medicine.

3. The method for preparing traditional Chinese medicine-derived carbon dots based on genipin as described in claim 2, characterized in that, In S2, the reaction temperature is 180℃ and the reaction time is 2 hours.

4. The method for preparing traditional Chinese medicine-derived carbon dots based on genipin as described in claim 2, characterized in that, In S3, separation and purification were performed by filtration through a 0.22 μm aqueous filter membrane, and the filtrate was centrifuged at 10000-12000 rpm for 10 min, and the supernatant was collected.

5. The method for preparing traditional Chinese medicine-derived carbon dots based on genipin as described in claim 2, characterized in that, In S3, the purified solution is placed in a dialysis bag and dialyzed for 24 hours. The dialysate is replaced every 12 hours. The dialysate is deionized water, and the molecular weight cutoff of the dialysis bag is 1000 Da.

6. The use of the genipin-based traditional Chinese medicine-derived carbon dots as described in claim 1 in the preparation of medicaments for the prevention and / or treatment of Parkinson's disease.

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