Aerosol CPP test recording device

Abstract

An aerosol CPP test recording device comprises a box-type device main body, and an optical fiber signal collector (103) and a training chamber module (300) are arranged in the device main body. According to the aerosol CPP test recording device and the use method thereof, the device is designed in combination with optical fiber recording, mouse position preference recording and analysis can be carried out, and dopamine signal changes when a mouse changes the position environment can be detected.

Description

Technical Field

[0001] The invention relates to the field of testing and recording devices for animal inhalation drug administration, in particular to an aerosol CPP testing and recording device. Background Art

[0002] The conditioned place preference experiment (CPP) began in 1979, and its equipment and methods have been continuously improved and perfected. Many scholars have used this method to analyze the reinforcing effects of various psychoactive drugs, proving that the results obtained are consistent with the results of experiments such as animal self-administration. Therefore, it is believed that this method is one of the simple and reliable methods for screening drugs with abuse potential. Many scholars have also used this method to explore the neural mechanism of drug reinforcement effects and obtained beneficial results. In recent years, it has been widely used as an effective method for evaluating the potential for drug psychotropic dependence.

[0003] In the conditioned place preference experiment, a rectangular box with two or three spaces is generally used. The two spaces constitute two small boxes of equal size that are approximately cube-shaped. The colors of the two boxes are different, mostly black and white, and some are gray and white. The four walls of the two boxes are smooth, the floor surfaces are rough and smooth respectively, there is a passage between the two boxes, and the top is covered. To avoid noise interference, the experiment is carried out under soundproof conditions. The commonly used procedure is: after the test drug is given to the animal, the animal is placed in a box on one side, and the passage between the two boxes is closed, and the animal is taken out after a predetermined time. The animal is given a control agent (such as saline) at an interval of 4-8h on the same day or at the same time period on the second day, and is placed in the box on the other side. The above cycle is repeated for multiple cycles, and the animal is finally tested. The animal is gently placed in the middle of the two boxes without any drug, so that the animal can shuttle freely between the two boxes, and the time the animal stays in the two boxes within the specified time is observed and recorded. The side with a longer stay time is considered the preferred side, and the side with a shorter stay time is the aversion side. The statistical results can be compared with the results of the pre-experiment before the conditioning training, or compared with the control group. In recent years, many scholars have proposed improvements to the experimental method of conditioned place preference. Among them, Hasenohi et al. proposed that the circular open-field device can be modified to avoid the natural preference of animals for the box environment on one side when using ordinary devices, thereby reducing experimental errors. Despite this, most studies currently still use traditional rectangular shuttle boxes or devices connected to black and white boxes.

[0004] At present, the existing CPP devices are difficult to combine with fiber optic recording, which makes it inconvenient to perform fiber optic recording of dopamine signals in the animal brain, and it is also inconvenient to perform CPP training and testing. Summary of the invention

[0005] In order to overcome the existing defects, the present invention proposes an aerosol CPP test and recording device.

[0006] An aerosol CPP test and recording device comprises a box-shaped device body, in which an optical fiber signal collector and a training chamber module are arranged.

[0007] The device body includes a shell, a front door frame is located on the front of the device body, the middle of the door frame is the front door glass, the door can be opened by a handle located at the bottom of the door frame, and the industrial control all-in-one machine is arranged on one side of the shell through a display cantilever.

[0008] Among them, the optical fiber signal collector is located at the top of the device body, the optical fiber signal collector is connected to the optical fiber to collect dopamine signals, and the optical fiber suspension is located at the upper end of the device box, which is used for optical fiber suspension and recording device fixation.

[0009] Wherein, a box positioning module is arranged on the inner wall of the device body, a positioning pin is arranged on the bottom surface of the box frame in the positioning module, and the training chamber module is fixed on the box frame through the positioning pin.

[0010] The training chamber module includes a training white box, a connecting channel and a training black box part. The training black box and the training white box are assembled through the connecting channel to accommodate the animal.

[0011] Preferably, there are multiple training chamber modules.

[0012] The bottom plate is located at the bottom of the device body, the foot pad is located below the bottom plate and is used to support the device, and the power switch is located on the outside of one side of the device body.

[0013] The device is particularly suitable for rodents, but is also suitable for other experimental animals.

[0014] The aerosol CPP test recording device and its use method provided in the present application are designed in combination with optical fiber recording, which can record and analyze the position preference of mice, and can be used to detect changes in dopamine signals when mice change their position environment.

[0015] Compared with the prior art, the present invention assembles and disassembles exposure boxes of different colors, which is convenient for animal training in aerosol CPP experiments, enables subsequent position preference analysis and detection of dopamine signal changes, and further infers drug effects, improves the instrument for aerosol exposure drug administration, and facilitates the development of optical fiber recording to analyze the trend of dopamine signal changes during drug seeking. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Schematic diagram of aerosol CPP test recording device.

[0017] Figure 2This is a schematic diagram of the cabinet positioning module structure.

[0018] Figure 3 Schematic diagram of the training chamber module structure.

[0019] Figure 4 Schematic diagram of the operation flow of the aerosol CPP test recording device. DETAILED DESCRIPTION

[0020] The aerosol CPP testing and recording device provided by the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0021] Figure 1-3 As shown, an aerosol CPP test and recording device includes a box-shaped device body, in which an optical fiber signal collector 103 and a training chamber module 300 are arranged.

[0022] The device body includes a shell 107, a front door frame 101 located in front of the device body, a front door glass 102 in the middle of the door frame, and the box door can be opened by a handle 112 located at the bottom of the door frame. The industrial control all-in-one machine 105 is arranged on one side of the shell 107 through a display cantilever 106.

[0023] The optical fiber signal collector 103 is located at the top of the device body, and the optical fiber signal collector 103 is connected to the optical fiber 111 to collect dopamine signals. The optical fiber hanger 104 is located at the upper end of the device box, and is used to hang the optical fiber 111 and fix the recording device 113.

[0024] A box positioning module 200 is disposed on the inner wall of the device body. A positioning pin 202 is disposed on the bottom surface of a box frame 201 in the positioning module 200 . The training chamber module 300 is fixed to the box frame 201 through the positioning pin 202 .

[0025] The training chamber module 300 includes a training white box 301 , a connecting channel 302 and a training black box 303 , and the training black box 303 and the training white box 301 are assembled through the connecting channel 302 .

[0026] The number of the training chamber modules 300 is multiple.

[0027] The bottom plate 109 is located at the bottom of the device body, the foot pad 110 is located below the bottom plate and is used to support the device, and the power switch 108 is located outside one side of the device body.

[0028] Specific implementation methods

[0029] like Figure 4 As shown, in actual work,

[0030] Step 1: Turn on the power switch, open the fiber optic signal collection software in the industrial computer, set it up, and start the recording device at the same time.

[0031] Step 2: Open the front door of the device, connect the optical fiber to the experimental animal, and gently place it into the connecting channel between the chambers, and close the front door of the device.

[0032] Step 3: After the scheduled time is reached, pause the recording of the optical fiber signal, open the front door of the device, take out the animal and remove the optical fiber, and gently put it back into the cage.

[0033] Step 4: Clean the inside of the device and the chamber, put the optical fiber back into the optical fiber hanger, and copy the data.

[0034] Carry out steps 2 to 4 in sequence to complete the subsequent experimental group / control group test records.

[0035] The device allows animals to move freely in the chamber, reducing the occurrence of negative emotions such as anxiety and depression in animals, ensuring the accuracy of optical fiber signal recording, and the device box is sealed and soundproof, reducing the impact of external sounds on experimental animals and improving the accuracy of experimental results;

[0036] Batch processing can test and record multiple objects at the same time, reducing the influence of other variables in the group and reducing experimental errors;

[0037] The top of the chamber and the top of the connecting channel are not blocked, which prevents the optical fiber recording line from being blocked as the mouse moves, further affecting its judgment and causing experimental errors;

[0038] The detachable separation chamber is easy to clean and convenient for the next group of experiments;

[0039] The device combines CPP test records with optical fiber signal records and is controlled by an industrial control integrated computer. The experimental steps are convenient and the space occupied by the experimental device is reduced.

[0040] The device is easy to operate, with an elegant overall design and a glass front door for observing the animal's status. It can simultaneously record fiber optic signals and position preference records and analyses for mice.

[0041] Finally, it should be noted that the above embodiments are only used to describe the technical solution of the present invention rather than to limit the technical method. The present invention can be extended to other modifications, changes, applications and embodiments in application, and therefore all such modifications, changes, applications and embodiments are considered to be within the scope of the present invention.

Claims

1. An aerosol CPP test and recording device, comprising a box-type device body, characterized in that: An optical fiber signal collector (103) and a training chamber module (300) are arranged in the main body of the device. The optical fiber signal collector (103) is located at the top of the main body of the device. The optical fiber signal collector (103) is connected to the optical fiber (111) to collect dopamine signals. The optical fiber hanger (104) is located at the upper end of the device box and is used to hang the optical fiber (111) and fix the recording device (113). The training chamber module (300) includes a training white box (301), a connecting channel (302) and a training black box (303). The training black box (303) is assembled with the training white box (301) through the connecting channel (302), wherein the tops of the training white box (301), the connecting channel (302) and the training black box (303) are not blocked.

2. The aerosol CPP test and recording device according to claim 1, characterized in that: The device body comprises a housing (107), a front door frame (101) located at the front of the device body, a front door glass (102) in the middle of the door frame, and the door can be opened by a handle (112) located at the bottom of the door frame. The industrial control all-in-one machine (105) is arranged on one side of the housing (107) through a display cantilever (106).

3. The aerosol CPP test and recording device according to claim 1, characterized in that: A box positioning module (200) is arranged on the inner wall of the device body, a positioning pin (202) is arranged on the bottom surface of the box frame (201) in the positioning module (200), and the training chamber module (300) is fixed to the box frame (201) via the positioning pin (202).

4. The aerosol CPP test and recording device according to claim 1, characterized in that: The training chamber modules (300) are multiple.

5. The aerosol CPP test and recording device according to claim 2, characterized in that: The bottom plate (109) is located at the bottom of the device body, the foot pad (110) is located below the bottom plate and is used to support the device, and the power switch (108) is located outside one side of the device body.

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