Sampling device of cell suspension culture tank

Abstract

The utility model discloses a sampling device for a cell suspension culture tank. The sampling device comprises a suspension cell sap sampling mechanism, the suspended cell sap sampling mechanism comprises a lower sampling tube assembly, the lower sampling tube assembly comprises a lower long sampling tube, and the bottom of the lower long sampling tube is communicated with a sampling nozzle tube; the lower sampling tube assembly is assembled and communicated with an upper long tube; a plurality of sampling liquid storages are assembled and connected to the upper long pipe; a plurality of valves corresponding to the sampling liquid storages are assembled and connected to the upper long pipe; in the sampling process, the upper long pipe is communicated with a negative pressure device in advance, the sampling liquid storage device is sucked to be in a negative pressure state, then the valves are kept closed, and in the sampling process, sampling is carried out after the sampling liquid storage device and the corresponding valves are selected to be opened. According to the device, the defect that the sampling device is easy to pollute due to frequent sampling and air exposure is overcome, and synchronous multiple sampling or interval multiple sampling is realized.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture sampling, in particular to a cell suspension culture tank sampling device. Background Art

[0002] Cell culture is to provide material conditions for cell proliferation through biotechnology, and then culture the cells under material conditions according to biological culture technology. Cell culture is very important in the biological field. Cell culture technology can be used to culture antibody cells and immune cells, and then can be used in fields such as vaccines. The above method is a conventional application in cell culture technology, and cell culture technology also has many other applications in the biological field, such as cloning antibodies.

[0003] Cell culture is usually carried out in culture tanks. Specifically, necessary nutrients for cell proliferation are added to the culture tanks, and during the culture process, necessary growth conditions for cell growth, such as temperature and oxygen content, are provided.

[0004] During the cell culture process, it is necessary to repeatedly sample the culture fluid (including cells) at regular intervals. The current sampling method is to repeatedly insert a sampling tube into the culture tank to take samples. However, the disadvantage of this method is that when the sampling tube is frequently inserted for sampling, it is easy to carry bacteria to contaminate the culture fluid. As a result, during the culture, it is easy to completely contaminate the material system in the entire culture tank and make it useless. Utility Model Content

[0005] Based on the above background, the purpose of the utility model is to provide a sampling device for a cell suspension culture tank.

[0006] In order to achieve the above objectives, the utility model adopts the following technical solutions:

[0007] A cell suspension culture tank sampling device, comprising a suspended cell fluid sampling mechanism;

[0008] The suspended cell fluid sampling mechanism comprises a lower sampling tube assembly, wherein the lower sampling tube assembly comprises a lower long sampling tube, and the bottom of the lower long sampling tube is connected to a sampling nozzle tube;

[0009] The lower sampling tube assembly is connected to the upper long tube;

[0010] The upper long tube is equipped with a plurality of sampling liquid storage devices;

[0011] The upper long tube is equipped with a plurality of valves corresponding to the sampling liquid storage device;

[0012] During the sampling process, the upper long tube is pre-connected to a negative pressure device to draw the sampling liquid storage device into a negative pressure state. Subsequently, the valve is kept closed. During the sampling process, the sampling liquid storage device and the corresponding valve are opened to perform sampling.

[0013] Preferably, the sampling nozzle tube is in the shape of a vertical cone.

[0014] Preferably, a plurality of obliquely arranged outwardly extending stirring plates are welded on the outer side wall of the sampling nozzle tube;

[0015] During the sampling process, the liquid at the sampling position is stirred by an externally extended stirring plate to prevent solid matter from being sucked into the sampling nozzle and clogging it.

[0016] Preferably, the upper long tube is sequentially connected to a first sampling liquid storage device, a second sampling liquid storage device, a third sampling liquid storage device, a fourth sampling liquid storage device, and a fifth sampling liquid storage device.

[0017] Preferably, the upper long tube is sequentially equipped with a first valve for controlling the liquid inlet into the first sampling liquid storage;

[0018] A second valve for controlling liquid inlet into the second sampling liquid storage;

[0019] A third valve for controlling liquid inlet into the third sampling liquid storage;

[0020] a fourth valve for controlling liquid inlet into a fourth sampling liquid storage;

[0021] a fifth valve for controlling liquid inlet into a fifth sampling liquid storage;

[0022] And a sixth valve for controlling vacuum extraction.

[0023] Preferably, the first sampling liquid storage device, the second sampling liquid storage device, the third sampling liquid storage device, the fourth sampling liquid storage device, and the fifth sampling liquid storage device all include sampling bottles;

[0024] The top of the sampling bottle is connected to a connecting threaded seat, and the bottom of the upper long tube is connected to a connecting tube;

[0025] The connecting pipe is connected to a mounting seat, and the connecting threaded seat is threadedly connected to the mounting seat;

[0026] The sampling bottle is connected to the mounting seat via a connecting thread seat.

[0027] Preferably, the connecting pipes are all equipped with control valves.

[0028] Preferably, the rear end of the upper long tube is equipped with a cleaning assembly;

[0029] The cleaning assembly includes a cleaning bottle, the cleaning bottle is connected to a cleaning liquid pipeline, the liquid inlet end of the cleaning liquid pipeline is located at the lower end of the cleaning bottle; the liquid outlet end of the cleaning liquid pipeline is connected to the upper long tube;

[0030] The cleaning component also includes a compressed air inlet pipe connected to the cleaning bottle, and the air outlet end of the compressed air inlet pipe is located at an upper position in the cleaning bottle.

[0031] Preferably, the liquid outlet end of the upper long tube is connected to a joint tube.

[0032] The utility model has the following beneficial effects:

[0033] 1. During the working process, all the control valves connected to each connecting pipe are opened. At this time, the first valve is closed, and the remaining second valve to sixth valve are opened. The negative pressure pump works to form a vacuum in the first sampling liquid storage device to the fifth sampling liquid storage device. At this time, all the above-mentioned open valves are closed. During the sampling process, the first valve corresponding to the first sampling liquid storage device (the control valve on the connecting pipe is opened synchronously) is opened, and the sample liquid is sucked into the first sampling liquid storage device under the action of the first sampling liquid storage device in a negative pressure state. Close the control valve of the connecting pipe on the first sampling liquid storage device, and gradually open the second valve to the fifth valve, and then sample and store them one by one.

[0034] 2. The above method can realize that during the sampling process, there is no need to repeatedly take the sampling device out of the culture solution and release the sample solution. This avoids the technical defect of frequent contact with air and pollution. In addition, the above device can realize separate sampling. When sampling, different parts and different liquid levels in the culture system can be sampled. This greatly improves the sampling efficiency during the cell culture process. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0036] Figure 1 It is a schematic diagram of the dispersed structure in the embodiment of the utility model;

[0037] Figure 2 This is a schematic diagram of the structure of the sampling nozzle tube in the embodiment of the utility model;

[0038] Figure 3 This is a schematic diagram of the structure of the cleaning component in the embodiment of the utility model;

[0039] Figure 4 For the utility model embodiment Figure 1 Schematic diagram of the plane structure in ;

[0040] Figure 5 It is a schematic diagram of the overall structure of an embodiment of the utility model.

[0041] The realization of the purpose, functional features and advantages of the utility model will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0042] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0043] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0044] In addition, in the present utility model, descriptions such as "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present utility model.

[0045] Example 1

[0046] like Figure 1-5 As shown, a sampling device for a cell suspension culture tank includes a suspended cell fluid sampling mechanism. The suspended cell fluid sampling mechanism can be used to simultaneously sample multiple samples during the sampling process, or to sample multiple samples separately in different time periods. In addition, during the sampling process, it is not necessary to repeatedly remove the sampling structure from the culture fluid system, expose it to the air after sampling, release the sample, and sample again, which can easily contaminate the entire cell material system.

[0047] Specifically, the above-mentioned suspended cell fluid sampling mechanism includes a lower sampling tube assembly, which includes a lower long sampling tube 1, and the bottom of the lower long sampling tube 1 is connected to a sampling nozzle tube 2. At the same time, the lower sampling tube assembly is assembled and connected to an upper long tube 3 (the lower long sampling tube 1 is connected to the upper long tube 3 in a threaded connection manner, which is convenient for disassembly and subsequent cleaning and replacement); the upper long tube 3 is assembled and connected to a plurality of sampling liquid storage devices. Specifically, the upper long tube 3 is sequentially connected to a first sampling liquid storage device 51, a second sampling liquid storage device 52, a third sampling liquid storage device 53, a fourth sampling liquid storage device 54, and a fifth sampling liquid storage device 55.

[0048] The main structure of the above-mentioned sampling liquid storage device is a glass sampling bottle. Specifically, the way it is connected to the upper long tube 3 is as follows: the top of the sampling bottle is connected to a connecting threaded seat A (the connecting threaded seat A has a through-hole structure), and the bottom of the upper long tube 3 is connected to a connecting pipe B; the connecting pipe B is connected to a mounting seat 37 (a threaded groove is provided on the mounting seat 37, and a connecting hole is provided in the groove to connect to the upper long tube 3), and the connecting threaded seat A is threadedly connected to the mounting seat 37. Specifically, through the cooperation of the threaded structure, this method facilitates the subsequent removal of the sample.

[0049] At the same time, in order to control the sample liquid to enter the corresponding sampling liquid storage, according to the existing conventional method, each connecting tube B is equipped with a control valve (not shown in the figure), and the corresponding control valve is opened, and the corresponding sampling liquid storage is connected to the long tube 3.

[0050] At the same time, the upper long tube 3 is equipped with a plurality of valves corresponding to the sampling liquid storage devices; specifically, the upper long tube 3 is sequentially equipped with a first valve 31 for controlling the liquid inlet of the first sampling liquid storage device 51; a second valve 32 for controlling the liquid inlet of the second sampling liquid storage device 52; a third valve 33 for controlling the liquid inlet of the third sampling liquid storage device 53; a fourth valve 34 for controlling the liquid inlet of the fourth sampling liquid storage device 54; and a fifth valve 35 for controlling the liquid inlet of the fifth sampling liquid storage device 55.

[0051] At the same time, the liquid outlet end of the upper long tube 3 is connected with a joint tube 4. According to the existing conventional method, the upper long tube 3 is connected with a negative pressure device (specifically, connected to the connection pipe of the negative pressure pump of the negative pressure device kettle through the joint tube 4). At the same time, in order to control the vacuum, the upper long tube 3 is equipped with a sixth valve 36 for controlling vacuuming. At the same time, according to the existing conventional method, before the compressed air enters the cleaning bottle, a 0.2 micron air filter should be installed on the compressed air inlet pipe to ensure that the compressed air entering the cleaning bottle is sterile.

[0052] During operation, the control valves connected to each connecting pipe B are opened. At this time, the first valve 31 is closed, and the remaining second valves 32 to sixth valves 36 are opened. The negative pressure pump works to form a vacuum in the first sampling liquid storage 51 to the fifth sampling liquid storage 55. At this time, all the above-mentioned open valves are closed.

[0053] During the sampling process, the first valve 31 corresponding to the first sampling liquid storage 51 (the control valve on the connecting pipe B is opened synchronously) is opened, and the sample liquid is sucked into the first sampling liquid storage 51 under the action of the first sampling liquid storage 51 in a negative pressure state.

[0054] The control valve of the connecting pipe B on the first sampling liquid storage 51 is closed, and the second valve 32 to the fifth valve 35 are gradually opened, and samples are taken and stored one by one.

[0055] The above method realizes that during the sampling process, there is no need to repeatedly take the sampling device out of the culture solution and release the sample solution. This avoids the technical defect of frequent contact with air and contamination. In addition, the above device can realize separate sampling. When sampling, different parts and different liquid levels in the culture system can be sampled. This greatly improves the sampling efficiency during the cell culture process.

[0056] Example 2

[0057] like Figure 1-5 As shown, in this embodiment, based on the structure of the embodiment 1, the shape of the sampling nozzle tube 2 is a vertical cone (that is, the sampling nozzle tube 2 includes a conical nozzle tube portion 21). At the same time, a plurality of inclined externally extending stirring plates 22 are welded on the outer side wall of the nozzle tube portion 21. The inclination direction of the externally extending stirring plates 22 corresponds to the conical structure of the sampling nozzle tube 2.

[0058] This method is implemented in the sampling process. During the sampling process, the liquid at the sampling position is stirred by the externally extended stirring plate 22 to prevent solid matter from being sucked into the sampling nozzle tube 2 and clogging it.

[0059] At the same time, in order to facilitate operation, a handle 11 is fixedly connected to the uppermost end of the lower long sampling tube 1 to facilitate pulling and shaking during the sampling process.

[0060] Example 3

[0061] like Figure 1-5 As shown, based on the structure of Example 1, after sampling, after the sample is released from the sampling liquid storage, in order to facilitate synchronous cleaning, the rear end of the upper long tube 3 is equipped with a cleaning component.

[0062] Specifically, the cleaning assembly includes a cleaning bottle 6, the cleaning bottle 6 is connected to a cleaning liquid pipeline 61 (according to the existing method, a valve is installed on the cleaning liquid pipeline 61), the liquid inlet end of the cleaning liquid pipeline 61 is located at the lower end of the cleaning bottle; the liquid outlet end of the cleaning liquid pipeline 6 is connected to the upper long tube 3; the cleaning assembly also includes a compressed air inlet pipe 62 connected to the cleaning bottle ( According to the existing conventional method, a 0.2 micron air filter is installed on the compressed air inlet pipe 62 to ensure that the compressed air entering the cleaning bottle is sterile and also a measure to prevent contamination. ) The outlet end of the compressed air inlet pipe 62 is located above the cleaning bottle 6 (the cleaning bottle 6 contains sterile saline).

[0063] According to the existing conventional method, compressed air is introduced into the compressed air inlet pipe (such as connected to an air compressor for compressing air, and a high-pressure gas pump is used to increase the pressure in the cleaning bottle), and the above-mentioned second valve 32 (the first valve 31 is closed) to the fifth valve 35 are gradually opened, and the control valve on the connecting pipe B (not shown in the figure) is used to pump the cleaning liquid into the cleaning bottle.

[0064] Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by technicians in this technical field within the essential scope of the present invention should also fall within the protection scope of the present invention.

Claims

1. A sampling device for a cell suspension culture tank, characterized in that: Including a suspended cell fluid sampling mechanism; The suspended cell fluid sampling mechanism comprises a lower sampling tube assembly, wherein the lower sampling tube assembly comprises a lower long sampling tube, and the bottom of the lower long sampling tube is connected to a sampling nozzle tube; The lower sampling tube assembly is connected to the upper long tube; The upper long tube is equipped with a plurality of sampling liquid storage devices; The upper long tube is equipped with a plurality of valves corresponding to the sampling liquid storage device; During the sampling process, the upper long tube is pre-connected to a negative pressure device to draw the sampling liquid storage device into a negative pressure state. Subsequently, the valve is kept closed. During the sampling process, the sampling liquid storage device and the corresponding valve are opened to perform sampling.

2. The cell suspension culture tank sampling device according to claim 1, characterized in that: The shape of the sampling nozzle is a vertical cone.

3. The cell suspension culture tank sampling device according to claim 1, characterized in that: A plurality of obliquely arranged outwardly extending stirring plates are welded on the outer side wall of the sampling nozzle tube; During the sampling process, the liquid at the sampling position is stirred by an externally extended stirring plate to prevent solid matter from being sucked into the sampling nozzle and clogging it.

4. The cell suspension culture tank sampling device according to claim 1, characterized in that: The upper long tube is connected in sequence with a first sampling liquid storage device, a second sampling liquid storage device, a third sampling liquid storage device, a fourth sampling liquid storage device, and a fifth sampling liquid storage device.

5. The cell suspension culture tank sampling device according to claim 4, characterized in that: The upper long tube is sequentially equipped with a first valve for controlling the liquid inlet into the first sampling liquid storage; A second valve for controlling liquid inlet into the second sampling liquid storage; A third valve for controlling liquid inlet into the third sampling liquid storage; a fourth valve for controlling liquid inlet into a fourth sampling liquid storage; a fifth valve for controlling liquid inlet into a fifth sampling liquid storage; And a sixth valve for controlling vacuum extraction.

6. The cell suspension culture tank sampling device according to claim 5, characterized in that: The first sampling liquid storage device, the second sampling liquid storage device, the third sampling liquid storage device, the fourth sampling liquid storage device, and the fifth sampling liquid storage device all include sampling bottles; The top of the sampling bottle is connected to a connecting threaded seat, and the bottom of the upper long tube is connected to a connecting tube; The connecting pipe is connected to a mounting seat, and the connecting threaded seat is threadedly connected to the mounting seat; The sampling bottle is connected to the mounting seat via a connecting thread seat.

7. The cell suspension culture tank sampling device according to claim 6, characterized in that: The connecting pipes are all equipped with control valves.

8. The cell suspension culture tank sampling device according to claim 1, characterized in that: The rear end of the upper long tube is connected with a cleaning assembly; The cleaning assembly includes a cleaning bottle, the cleaning bottle is connected to a cleaning liquid pipeline, the liquid inlet end of the cleaning liquid pipeline is located at the lower end of the cleaning bottle; the liquid outlet end of the cleaning liquid pipeline is connected to the upper long tube; The cleaning component also includes a compressed air inlet pipe connected to the cleaning bottle, and the air outlet end of the compressed air inlet pipe is located at an upper position in the cleaning bottle.

9. The cell suspension culture tank sampling device according to claim 1, characterized in that: The liquid outlet end of the upper long tube is connected with a joint pipe.

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